<?xml version="1.0" encoding="UTF-8"?>
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<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>P54029</accession>
    <name>RS10_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps10</name>
        <name type="ordered locus">MJ0322</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98306" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98306.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64340">
        <property type="entry name" value="C64340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_048201890.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54029"/>
    <dbReference type="SMR" id="P54029"/>
    <dbReference type="STRING" id="243232.MJ_0322"/>
    <dbReference type="PRIDE" id="P54029"/>
    <dbReference type="EnsemblBacteria" id="AAB98306">
        <property type="protein sequence ID" value="AAB98306"/>
        <property type="gene ID" value="MJ_0322"/>
    </dbReference>
    <dbReference type="GeneID" id="24891501"/>
    <dbReference type="KEGG" id="mja:MJ_0322"/>
    <dbReference type="eggNOG" id="arCOG01758">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122625_0_1_2"/>
    <dbReference type="InParanoid" id="P54029"/>
    <dbReference type="OMA" id="QFEIRVH"/>
    <dbReference type="OrthoDB" id="107993at2157"/>
    <dbReference type="PhylomeDB" id="P54029"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_S10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_S10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_S10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_S10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_S10_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="PTHR11700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="SSF54999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000146646" description="30S ribosomal protein S10">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="101" mass="11673" checksum="7676C40BA48DAF07" modified="2001-12-13" version="2">MQRARIKLSSTDHKVLDEICRQIKEIAEKTGVDISGPIPLPTKVLRVVTRKSPDGEGSSTFDRWTMKIHKRLIDIDADERALRHIMKIRIPDNVQIEIQFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>P54038</accession>
    <name>RL24_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl24</name>
        <name type="ordered locus">MJ0467</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64358">
        <property type="entry name" value="C64358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869967.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54038"/>
    <dbReference type="SMR" id="P54038"/>
    <dbReference type="STRING" id="243232.MJ_0467"/>
    <dbReference type="EnsemblBacteria" id="AAB98456">
        <property type="protein sequence ID" value="AAB98456"/>
        <property type="gene ID" value="MJ_0467"/>
    </dbReference>
    <dbReference type="GeneID" id="1451329"/>
    <dbReference type="KEGG" id="mja:MJ_0467"/>
    <dbReference type="eggNOG" id="arCOG04094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093240_2_1_2"/>
    <dbReference type="InParanoid" id="P54038"/>
    <dbReference type="OMA" id="VRIMRGD"/>
    <dbReference type="OrthoDB" id="107015at2157"/>
    <dbReference type="PhylomeDB" id="P54038"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_L24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="KOW_RPL26/RPL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_L26/L24P_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="PTHR11143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130770" description="50S ribosomal protein L24">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <sequence length="120" mass="14103" checksum="0FA953D929D18176" modified="1996-10-01" version="1">MAFTKSKQPRKQRKALFNAPLHLRRKVMSAMLSKELKEKLGKNAIPVRKGDVVRIMRGDFKGLEGEVIKVDLKRYRIYVEGANNKRQDGREVPYPIHPSNVMIIKLYDKDEKRFKHIKNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-13" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>P60819</accession>
    <name>SELD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Selenide, water dikinase</fullName>
            <ecNumber evidence="1">2.7.9.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Selenium donor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Selenophosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">selD</name>
        <name type="ordered locus">MJ1591</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="J. Mol. Biol." volume="266" first="637" last="641">
            <title>Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion.</title>
            <authorList>
                <person name="Wilting R."/>
                <person name="Schorling S."/>
                <person name="Persson B.C."/>
                <person name="Boeck A."/>
            </authorList>
            <dbReference type="PubMed" id="9102456"/>
            <dbReference type="DOI" id="10.1006/jmbi.1996.0812"/>
        </citation>
        <scope>PROBABLE SELENOCYSTEINE AT SEC-19</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Synthesizes selenophosphate from selenide and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + hydrogenselenide = AMP + 2 H(+) + phosphate + selenophosphate</text>
            <dbReference type="Rhea" id="RHEA:18737"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:29317"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per monomer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the selenophosphate synthase 1 family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="P60819"/>
    <dbReference type="OMA" id="DNEPKYG"/>
    <dbReference type="PhylomeDB" id="P60819"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00625">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023061">
        <property type="entry name" value="SelD_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="PTHR10256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0711">Selenium</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000127645" description="Selenide, water dikinase">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="1">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="2">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="349" mass="38040" checksum="93B43EB552563680" modified="2008-02-26" version="2">MERGNEKIKLTELVKLHGUACKLPSTELEFLVKGIVTDDDLLDKNILVGLGDDASIIKRNGLVIAKTVDVFTPIVDDPYIQGKIAACNSTSDIYAMGLLDIVGVLAIVGIPEKLPIHVVREMLKGFQDFCRENKTTIVGGHTILNPWPLIGGAVTGVGREEEVLTKAGVKVGDVLILTKPLGTQTAMALSRIPEEFKDLISITEEERDYIINKAIEIMTTSNRYALKALRKAEERVGDKIANALTDITGFGILGHSNEMAKNSNVLIEINLLPCIKRTPELSRLFGHALLDGYGAETAGGLLISAKEEYKDNLIDELEKAKCYAFEVGRVVKKGEGKAVLSKDVKVIEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>P81227</accession>
    <name>YE7B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1479.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1479.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81227"/>
    <dbReference type="STRING" id="243232.MJ_1479.1"/>
    <dbReference type="PRIDE" id="P81227"/>
    <dbReference type="EnsemblBacteria" id="AAB99501">
        <property type="protein sequence ID" value="AAB99501"/>
        <property type="gene ID" value="MJ_1479.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1479.1"/>
    <dbReference type="eggNOG" id="arCOG05086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2243898_0_0_2"/>
    <dbReference type="OMA" id="CCILHAY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107362" description="Uncharacterized protein MJ1479.1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <sequence length="129" mass="15505" checksum="4E41E257A58A8C69" modified="1998-07-15" version="1">MSSKPLYTILTVFNPIFIYFTKRDNLHTLRFKKDRAIKISEELFPDELCERCGRCCILHAYKTEDGIKTIYCEHLDPETKLCKVYKDRFKHRCLTVMEGILAGVFPKDCPYVKNLKNYEEPWFYRHLRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>P81233</accession>
    <name>Y79B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0795.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0795.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ1249.1, MJ0210.1 and MJ0785.1.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870304.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81233"/>
    <dbReference type="STRING" id="243232.MJ_0795.1"/>
    <dbReference type="PRIDE" id="P81233"/>
    <dbReference type="EnsemblBacteria" id="AAB98801">
        <property type="protein sequence ID" value="AAB98801"/>
        <property type="gene ID" value="MJ_0795.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451676"/>
    <dbReference type="KEGG" id="mja:MJ_0795.1"/>
    <dbReference type="eggNOG" id="arCOG09674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451435_0_0_2"/>
    <dbReference type="OMA" id="YANYLMF"/>
    <dbReference type="OrthoDB" id="138391at2157"/>
    <dbReference type="PhylomeDB" id="P81233"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107047" description="Uncharacterized protein MJ0795.1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="170" mass="19689" checksum="CE4B561CD16C01AA" modified="1998-07-15" version="1">MKGINPFYFYIGMALILASIVSILLITKSILLFILLAFGSLVGITLILIYISRKILKIDKGRLKKEVKRIFGNRVYKILRLMLVLGYAGFIYFSGTFYNSAVLFFIFIVAFTISEFYKTYRIRIYEKGILIEGIAFYSWEEIEKTTNKDKNQTILKIKGIPKKIVINEII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>P81301</accession>
    <name>Y323_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0323</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0323</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81301"/>
    <dbReference type="EnsemblBacteria" id="AAB98307">
        <property type="protein sequence ID" value="AAB98307"/>
        <property type="gene ID" value="MJ_0323"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0323"/>
    <dbReference type="HOGENOM" id="CLU_2447690_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106793" description="Uncharacterized protein MJ0323">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="89" mass="9228" checksum="F22EF5FDC5C2CE6C" modified="1998-06-01" version="1">MFLALTSIAIPAAIVIPISLIANLPNCGISLTFSMTIGFVGLILTIAASPVFKNCGFSSMTCPVLGSNFFNNSMNVHATCAVCAWKTGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>P81314</accession>
    <name>Y79C_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0796.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0796.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81314"/>
    <dbReference type="SMR" id="P81314"/>
    <dbReference type="STRING" id="243232.MJ_0796.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98802">
        <property type="protein sequence ID" value="AAB98802"/>
        <property type="gene ID" value="MJ_0796.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0796.1"/>
    <dbReference type="eggNOG" id="arCOG09675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1821057_0_0_2"/>
    <dbReference type="OMA" id="VINEVVY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107048" description="Uncharacterized protein MJ0796.1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="137" mass="15905" checksum="9FE225FF3A3C698E" modified="1998-07-15" version="1">MMIDNISNFDKVRAVVVAILLYIFIILVVDGSISSLIGKYITYPSDEYHIIEFYDFIHIIGFLLSLSISTYFSSKDIIKDFAKFFTIFFGITFILGITLFLGLTFFENHIPSMRGYTTLMLFFFLLNLFKKLDKITN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q57596</accession>
    <name>T1M1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Type I restriction enzyme MjaVII methylase subunit</fullName>
            <shortName>M protein</shortName>
            <ecNumber evidence="1">2.1.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Type I methyltransferase M.MjaVII</fullName>
            <shortName evidence="3">M.MjaVII</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0132</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">The subtype gamma methyltransferase (M) subunit of a type I restriction enzyme. The M and S subunits together form a methyltransferase (MTase) that methylates A-3 on the top and bottom strands of the sequence 5'-CAAN(7)TGG-3'. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg(2+) as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the N4/N6-methyltransferase family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAB98113" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98113.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64316">
        <property type="entry name" value="D64316"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57596"/>
    <dbReference type="STRING" id="243232.MJ_0132"/>
    <dbReference type="REBASE" id="3902">
        <property type="entry name" value="M.MjaVII"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98113">
        <property type="protein sequence ID" value="AAB98113"/>
        <property type="gene ID" value="MJ_0132"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0132"/>
    <dbReference type="eggNOG" id="arCOG02632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013049_1_0_2"/>
    <dbReference type="PhylomeDB" id="Q57596"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006306">
        <property type="term" value="P:DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022749">
        <property type="entry name" value="D12N6_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12161">
        <property type="entry name" value="HsdM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106710" description="Type I restriction enzyme MjaVII methylase subunit">
        <location>
            <begin position="1"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="251"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="281"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="335"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P08957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q89Z59"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="577" mass="67315" checksum="650D178C2FE6EC2F" modified="2022-02-23" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q57602</accession>
    <name>Y138_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative glutamine amidotransferase-like protein MJ0138</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0138</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64317">
        <property type="entry name" value="B64317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869631.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57602"/>
    <dbReference type="STRING" id="243232.MJ_0138"/>
    <dbReference type="DNASU" id="1450979"/>
    <dbReference type="EnsemblBacteria" id="AAB98121">
        <property type="protein sequence ID" value="AAB98121"/>
        <property type="gene ID" value="MJ_0138"/>
    </dbReference>
    <dbReference type="GeneID" id="1450979"/>
    <dbReference type="KEGG" id="mja:MJ_0138"/>
    <dbReference type="eggNOG" id="arCOG00107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_565768_0_0_2"/>
    <dbReference type="OMA" id="FHCHTYG"/>
    <dbReference type="OrthoDB" id="18243at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
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        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PTHR31559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106714" description="Putative glutamine amidotransferase-like protein MJ0138">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00605"/>
        </source>
    </evidence>
    <sequence length="482" mass="54464" checksum="60598EEE7C120409" modified="1997-11-01" version="1">MEIGILDIKGSLPLFEDFGNLPTKIITENNYKEIKDLDALIIPGGSLIESKSLNDDLKKEIINFNGYIIGICSGFQILAKKIDIGRKSSVPIIKEGLGLLDVEFSPLVCTDRVEFEVKKSIFGEGKGEGFHCHTYGNIEVVDKETKILTVSKVKKLNYKLGAEKEIISGAFKGKVFGTMVHNFLDNEFVRDNFLKHLGVTEDEKEEIFEKNKIIKDELKKRALKYRLNPKLLKENNKKDVNKKRGIILLATSSNSGKTFLTTALSSKLNGRVFVAKIGGDVRDIVPALYLLREKMTKYNSIKIGERGWVDVSKFLDYIKKSDYDYIIVEGVMGAFTAALKNISSYQIAKKLGFPVYIVSACNISGIEGAFVEAMAYYSLLKDIGIKVEGVILNKVYDWNSFNKLKSLAEKHNIKLYGVGKIANESRGLIPEVEIDYESFCRNAFNVDLEIEIPEVEINNHIKDEEDNFLERLDNWMENINKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57610</accession>
    <name>FER3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0146</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0146</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64318">
        <property type="entry name" value="C64318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869641.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57610"/>
    <dbReference type="SMR" id="Q57610"/>
    <dbReference type="STRING" id="243232.MJ_0146"/>
    <dbReference type="EnsemblBacteria" id="AAB98129">
        <property type="protein sequence ID" value="AAB98129"/>
        <property type="gene ID" value="MJ_0146"/>
    </dbReference>
    <dbReference type="GeneID" id="1450990"/>
    <dbReference type="KEGG" id="mja:MJ_0146"/>
    <dbReference type="eggNOG" id="arCOG00959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_3_2"/>
    <dbReference type="InParanoid" id="Q57610"/>
    <dbReference type="OMA" id="CARMCPD"/>
    <dbReference type="OrthoDB" id="124457at2157"/>
    <dbReference type="PhylomeDB" id="Q57610"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159131" description="Uncharacterized ferredoxin MJ0146">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="69" mass="7756" checksum="19FBAACD8A6FEEFB" modified="1997-11-01" version="1">MKIEINENFCKGCDICIVVCPRGVFEKSKKLNKKGIYPPIPVNPEKCTKCNLCILQCPDQAISIELSQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q57621</accession>
    <name>Y157_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0157</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0157</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64319">
        <property type="entry name" value="F64319"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57621"/>
    <dbReference type="STRING" id="243232.MJ_0157"/>
    <dbReference type="EnsemblBacteria" id="AAB98150">
        <property type="protein sequence ID" value="AAB98150"/>
        <property type="gene ID" value="MJ_0157"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0157"/>
    <dbReference type="eggNOG" id="arCOG04846">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159088_1_1_2"/>
    <dbReference type="InParanoid" id="Q57621"/>
    <dbReference type="OMA" id="DNNLMYR"/>
    <dbReference type="PhylomeDB" id="Q57621"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019209">
        <property type="entry name" value="DUF2098"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017099">
        <property type="entry name" value="UCP037053"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09871">
        <property type="entry name" value="DUF2098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037053">
        <property type="entry name" value="UCP037053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106721" description="Uncharacterized protein MJ0157">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <sequence length="96" mass="10954" checksum="C81160A577AB5272" modified="1996-11-01" version="1">MGYMDEVNIKVGDYVVYINTGTKGRVVDIRKDENGDIWVVLDNNLMYRPHLLRVIDKSKITERKEDIDEVVKKLEKEELEEGKLIDLDLGDACGAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q57623</accession>
    <name>NRPR_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Global nitrogen regulator NrpR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nitrogen regulatory protein R</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">nrpR</name>
        <name evidence="6" type="ordered locus">MJ0159</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="Structure" volume="18" first="1512" last="1521">
            <title>Structural underpinnings of nitrogen regulation by the prototypical nitrogen-responsive transcriptional factor NrpR.</title>
            <authorList>
                <person name="Wisedchaisri G."/>
                <person name="Dranow D.M."/>
                <person name="Lie T.J."/>
                <person name="Bonanno J.B."/>
                <person name="Patskovsky Y."/>
                <person name="Ozyurt S.A."/>
                <person name="Sauder J.M."/>
                <person name="Almo S.C."/>
                <person name="Wasserman S.R."/>
                <person name="Burley S.K."/>
                <person name="Leigh J.A."/>
                <person name="Gonen T."/>
            </authorList>
            <dbReference type="PubMed" id="21070950"/>
            <dbReference type="DOI" id="10.1016/j.str.2010.08.014"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 306-542</scope>
        <scope>DNA-BINDING</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional repressor of nitrogen fixation and assimilation genes. Binds to two tandem operators in the glnA and nif promoters, thereby blocking transcription of the genes.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Under nitrogen limitation, binding of the intracellular nitrogen metabolite 2-oxoglutarate to NrpR decreases the binding affinity of NrpR to DNA, leading to initiation of transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Adopts different quaternary structures in its active apo state compared with its inhibited 2-oxoglutarate-bound state. In the 2-oxoglutarate-bound state, NrpR is inhibited from binding to DNA because its DNA-binding domains are too far apart to recognize the operator DNA sequence.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NrpR family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64319">
        <property type="entry name" value="H64319"/>
    </dbReference>
    <dbReference type="PDB" id="3NEK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=306-542"/>
    </dbReference>
    <dbReference type="PDBsum" id="3NEK"/>
    <dbReference type="AlphaFoldDB" id="Q57623"/>
    <dbReference type="SMR" id="Q57623"/>
    <dbReference type="DIP" id="DIP-59016N"/>
    <dbReference type="STRING" id="243232.MJ_0159"/>
    <dbReference type="DNASU" id="1451006"/>
    <dbReference type="EnsemblBacteria" id="AAB98143">
        <property type="protein sequence ID" value="AAB98143"/>
        <property type="gene ID" value="MJ_0159"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0159"/>
    <dbReference type="eggNOG" id="arCOG02710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_507744_0_0_2"/>
    <dbReference type="InParanoid" id="Q57623"/>
    <dbReference type="OMA" id="FEDYEPV"/>
    <dbReference type="PhylomeDB" id="Q57623"/>
    <dbReference type="EvolutionaryTrace" id="Q57623"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1360">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002846">
        <property type="entry name" value="NRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038982">
        <property type="entry name" value="NrpR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036984">
        <property type="entry name" value="NrpR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013668">
        <property type="entry name" value="RNase_R_HTH_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41964">
        <property type="entry name" value="PTHR41964"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08461">
        <property type="entry name" value="HTH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01995">
        <property type="entry name" value="NRD1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000106723" description="Global nitrogen regulator NrpR">
        <location>
            <begin position="1"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Winged helix-turn-helix" evidence="4">
        <location>
            <begin position="12"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="NRD 1" evidence="5">
        <location>
            <begin position="85"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="NRD 2" evidence="5">
        <location>
            <begin position="321"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="325"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="340"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="346"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="356"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="359"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="375"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="386"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="397"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="425"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="440"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="445"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="450"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="463"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="481"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="500"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="510"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="523"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="533"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="536"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LZL7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21070950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="21070950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="21070950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000312" key="6">
        <source>
            <dbReference type="EMBL" id="AAB98143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3NEK"/>
        </source>
    </evidence>
    <sequence length="542" mass="61195" checksum="5C7FC0734338AE0F" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q57624</accession>
    <name>GATB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B</fullName>
            <shortName>Asp/Glu-ADT subunit B</shortName>
            <ecNumber>6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">gatB</name>
        <name type="ordered locus">MJ0160</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GatB/GatE family. GatB subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64320">
        <property type="entry name" value="A64320"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57624"/>
    <dbReference type="SMR" id="Q57624"/>
    <dbReference type="STRING" id="243232.MJ_0160"/>
    <dbReference type="EnsemblBacteria" id="AAB98144">
        <property type="protein sequence ID" value="AAB98144"/>
        <property type="gene ID" value="MJ_0160"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0160"/>
    <dbReference type="eggNOG" id="arCOG01718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019240_0_0_2"/>
    <dbReference type="InParanoid" id="Q57624"/>
    <dbReference type="OMA" id="ARKWWMG"/>
    <dbReference type="PhylomeDB" id="Q57624"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00121">
        <property type="entry name" value="GatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004413">
        <property type="entry name" value="Apn/Gln-ADT_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00133">
        <property type="entry name" value="gatB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148871" description="Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="472" mass="53736" checksum="2C14856409CBF03A" modified="1997-11-01" version="1">MGGNMEDVKMKCGLEIHVQIDTKSKLFCNCSTNYLDAEPNTNVCPVCLGLPGAKPLPPNKKAVEVAIMVAKMLGCKIVVDEDIYFQRKHYDYPDLPSGYQRTSTPIGVDGEFMGIGIHEVHLEEDPGQYNPSFGIVDYNRSGTPLIEIVTKPDIKSPEEAREFLKQLMTLFRYLGCLRGEGTMRADVNISIEYMGVQGNRVEVKNVNSIKGVYKVLKYELIRQKNIIKRGGEVKRETRAFLESQMITKAMRSKETAEDYRYIPDPDIQPIVISEKWVKEIEEKMPETPLAKKKRFVEEYGIDEEDAKVLVSDLDMAEMFEEVVKSLGVNKENVDLAVTWIRNELRRSLQYHKVDLYESGVKAEHIVELIKLIKEGVISQKIAKEIVDLLVINRGKKMPKELVEELGLTVIRDEDALVKAVEEAIKNNPKAVEDYLNGKKEALNFLMGQVMRLTRGRADPKRVIELLKERLDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57638</accession>
    <name>PELO_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein pelota homolog</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pelA</name>
        <name type="ordered locus">MJ0174</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64321">
        <property type="entry name" value="G64321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869669.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57638"/>
    <dbReference type="SMR" id="Q57638"/>
    <dbReference type="STRING" id="243232.MJ_0174"/>
    <dbReference type="EnsemblBacteria" id="AAB98159">
        <property type="protein sequence ID" value="AAB98159"/>
        <property type="gene ID" value="MJ_0174"/>
    </dbReference>
    <dbReference type="GeneID" id="1451021"/>
    <dbReference type="KEGG" id="mja:MJ_0174"/>
    <dbReference type="eggNOG" id="arCOG01741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023334_0_0_2"/>
    <dbReference type="InParanoid" id="Q57638"/>
    <dbReference type="OMA" id="DDLWHLK"/>
    <dbReference type="OrthoDB" id="90096at2157"/>
    <dbReference type="PhylomeDB" id="Q57638"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_1_Pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="Transl-rel_pelota"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PTHR10853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="SSF159065"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000143193" description="Protein pelota homolog">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <sequence length="347" mass="39624" checksum="2F1FBCA439EB47A2" modified="1996-11-01" version="1">MKIIEEIPQKNIIKLMPENLDDLWVLYNIIEEGDKIFAVTERRVQDKGDVIRADRGAKRKMFLGIEVKNVEFDENTKRVRILGTIIHGPDDVPLGSHHTIEIKPFDELSIEKNWKKWQIERIKEAIESSKRPKVLVVVMDDEEADIFEVRDYSIKEICSIKSHTSKKLDYKINEELKKEYYHEIAKVLSEYDVDNILVAGPGFAKNSFYNFISSQYPELKNKIVVESISTTSRAGLNEVIKRGIINRIYAESRVAKETQLIEKLLEEIAKKGLAVYGIDEVKKALEYSAIDTLLVSDSLVRNHEIEKIIDTTEEMGGKVVIVSSEHDAGKQLKALGGIAGLLRFPIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q57674</accession>
    <name>ATPL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable ATPase proteolipid chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0221</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64327">
        <property type="entry name" value="F64327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869717.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57674"/>
    <dbReference type="SMR" id="Q57674"/>
    <dbReference type="STRING" id="243232.MJ_0221"/>
    <dbReference type="EnsemblBacteria" id="AAB98207">
        <property type="protein sequence ID" value="AAB98207"/>
        <property type="gene ID" value="MJ_0221"/>
    </dbReference>
    <dbReference type="GeneID" id="1451071"/>
    <dbReference type="KEGG" id="mja:MJ_0221"/>
    <dbReference type="eggNOG" id="arCOG02455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237912_0_0_2"/>
    <dbReference type="InParanoid" id="Q57674"/>
    <dbReference type="OMA" id="NFIQLGA"/>
    <dbReference type="OrthoDB" id="116293at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="SSF81333"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0446">Lipid-binding</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000071731" description="Probable ATPase proteolipid chain">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="220" mass="21332" checksum="72DA35D2448BEAE6" modified="1996-11-01" version="1">MVDPLILGAVGAGLAVGIAGLGSGIGAGITGASGAGVVAEDPNKFGTAIVFQALPQTQGLYGFLVAILILFVFKTVSPWAMFAAGLAAGLAGLSAIGQGIAASAGLGAVAEDNSIFGKAMVFSVLPETQAIYGLLIAILLLVGVFKGNAGAETVAALGAGFAVGFAGLSGIGQGITAAGAIGATARDPDAMGKGLVLAVMPETFAIFGLLIAILIMLMIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57692</accession>
    <name>Y240_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative adenylate cyclase MJ0240</fullName>
            <ecNumber>4.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ATP pyrophosphate-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Adenylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0240</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could catalyze the biosynthesis of cyclic AMP (cAMP) from ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adenylyl cyclase CyaB family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64330">
        <property type="entry name" value="A64330"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869738.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57692"/>
    <dbReference type="SMR" id="Q57692"/>
    <dbReference type="STRING" id="243232.MJ_0240"/>
    <dbReference type="EnsemblBacteria" id="AAB98225">
        <property type="protein sequence ID" value="AAB98225"/>
        <property type="gene ID" value="MJ_0240"/>
    </dbReference>
    <dbReference type="GeneID" id="1451094"/>
    <dbReference type="KEGG" id="mja:MJ_0240"/>
    <dbReference type="eggNOG" id="arCOG01723">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105244_2_0_2"/>
    <dbReference type="InParanoid" id="Q57692"/>
    <dbReference type="OMA" id="QHPCRDF"/>
    <dbReference type="OrthoDB" id="123503at2157"/>
    <dbReference type="PhylomeDB" id="Q57692"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="PTHR21028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="SSF55154"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0115">cAMP biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106757" description="Putative adenylate cyclase MJ0240">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="CYTH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="175" mass="20821" checksum="7A85A90FD46F97F9" modified="1996-11-01" version="1">MIEVEIKVKIDDKNKVVEQLKKLGFKFIKKKFQEDIYFNGIDRDFRETDEALRIRDEDGNFFVTYKGPKIDKISKTREEIEVKIEDKEKMRQIFKKLGFKEVPPIRKIREIYKKEDIEASIDDVEGLGLFLELEKSISDINEKDKVLEEMMEILKALNISKDNIIRKSYLELRGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57757</accession>
    <name>SPEB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Agmatinase</fullName>
            <ecNumber evidence="1">3.5.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">speB</name>
        <name type="ordered locus">MJ0309</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Biochemistry" volume="51" first="3067" last="3078">
            <title>A new subfamily of agmatinases present in methanogenic Archaea is Fe(II) dependent.</title>
            <authorList>
                <person name="Miller D."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="22439800"/>
            <dbReference type="DOI" id="10.1021/bi300039f"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF CYS-71; CYS-136; CYS-151 AND CYS-229</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of putrescine from agmatine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>agmatine + H2O = putrescine + urea</text>
            <dbReference type="Rhea" id="RHEA:13929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16199"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="3.5.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 2 Fe(2+) ions per subunit. Can also use Mn(2+), with lower efficiency.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by putrescine and by the substrate analog arginine. Inactive when the purified enzyme is incubated with dithiothreitol followed by excess iodoacetic acid or N-ethylmaleimide.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">2.4 mM for agmatine</KM>
            <text evidence="1">kcat is 0.032 sec(-1).</text>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amine and polyamine biosynthesis; putrescine biosynthesis via agmatine pathway; putrescine from agmatine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms multimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.3.11" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64338">
        <property type="entry name" value="F64338"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869807.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57757"/>
    <dbReference type="SMR" id="Q57757"/>
    <dbReference type="STRING" id="243232.MJ_0309"/>
    <dbReference type="EnsemblBacteria" id="AAB98295">
        <property type="protein sequence ID" value="AAB98295"/>
        <property type="gene ID" value="MJ_0309"/>
    </dbReference>
    <dbReference type="GeneID" id="1451164"/>
    <dbReference type="KEGG" id="mja:MJ_0309"/>
    <dbReference type="eggNOG" id="arCOG01700">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039478_0_2_2"/>
    <dbReference type="InParanoid" id="Q57757"/>
    <dbReference type="OMA" id="YELTTIM"/>
    <dbReference type="OrthoDB" id="52310at2157"/>
    <dbReference type="PhylomeDB" id="Q57757"/>
    <dbReference type="BRENDA" id="3.5.3.11">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00534">
        <property type="reaction ID" value="UER00287"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, using agmatinase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="PTHR11358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00116">
        <property type="entry name" value="ARGINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0661">Putrescine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000173784" description="Agmatinase">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="24% of wild-type activity in the presence of DTT." evidence="1">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="89% of wild-type activity in the presence of DTT." evidence="1">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="5% of wild-type activity in the presence of DTT." evidence="1">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="96% of wild-type activity in the presence of DTT." evidence="1">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="22439800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="22439800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="22439800"/>
        </source>
    </evidence>
    <sequence length="284" mass="32477" checksum="0F76B94B1562236E" modified="1997-11-01" version="1">MEEHFIDLSKFMMANCPYEEAEGVIFSIPYDETTSFKPGAREGGNAIRTASWGLETYSPILDRDLAELKYCDLKDLDLYGSQEEIFGTIHSVSREILKENKKIIVFGGEHSITYPIIKAVKDIYDDFIVIQFDAHCDLRDEYLGNKLSHACVMRRVYELTKNIFQFGIRSGDKEEWDLARKNNLYLKMDLMNKDDLEYIKSLDKPIYVTIDIDVLDPAYAPGTGTPEPCGFSTRELFNSLYLLEEVKDKIIGFDIVEVSPIYDIANITAITAAKIARELMLMIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q57770</accession>
    <name>EF1A_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Elongation factor 1-alpha</fullName>
            <shortName evidence="2">EF-1-alpha</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Elongation factor Tu</fullName>
            <shortName evidence="2">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">tuf</name>
        <name type="ordered locus">MJ0324</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64340">
        <property type="entry name" value="D64340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869821.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57770"/>
    <dbReference type="SMR" id="Q57770"/>
    <dbReference type="STRING" id="243232.MJ_0324"/>
    <dbReference type="EnsemblBacteria" id="AAB98308">
        <property type="protein sequence ID" value="AAB98308"/>
        <property type="gene ID" value="MJ_0324"/>
    </dbReference>
    <dbReference type="GeneID" id="1451178"/>
    <dbReference type="KEGG" id="mja:MJ_0324"/>
    <dbReference type="eggNOG" id="arCOG01561">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007265_3_5_2"/>
    <dbReference type="InParanoid" id="Q57770"/>
    <dbReference type="OMA" id="AIRDMGM"/>
    <dbReference type="OrthoDB" id="13117at2157"/>
    <dbReference type="PhylomeDB" id="Q57770"/>
    <dbReference type="SABIO-RK" id="Q57770"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004160">
        <property type="entry name" value="Transl_elong_EFTu/EF1A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03143">
        <property type="entry name" value="GTP_EFTU_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000090980" description="Elongation factor 1-alpha">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="5"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="1">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="1">
        <location>
            <begin position="70"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="1">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="1">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="1">
        <location>
            <begin position="189"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <sequence length="428" mass="47280" checksum="0E375F776B8BA82E" modified="1997-11-01" version="1">MAKQKPVLNVAFIGHVDAGKSTTVGRLLYDSGAIDPQLLEKLKREAQERGKAGFEFAYVMDNLKEERERGVTIDVAHKKFETQKYEVTIVDCPGHRDFIKNMITGASQADAAVLVVDVNDAKTGIQPQTREHMFLARTLGIKQIAVAINKMDTVNYSQEEYEKMKKMLSEQLLKVLGYNPDQIDFIPTASLKGDNVVKRSENMPWYKGPTLVEALDKFQPPEKPTNLPLRIPIQDVYSITGVGTVPVGRVETGILRPGDKVVFEPAGVSGEVKSIEMHHEQIPQAEPGDNIGFNVRGVSKKDIKRGDVCGHPDNPPTVAEEFTAQIVVLQHPTAITVGYTPVFHAHTAQVACTFIELLKKLDPRTGQVIEENPQFLKTGDAAIVKIKPTKPMVIENVREIPQLGRFAIRDMGMTIAAGMAIDVKAKNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q57781</accession>
    <name>Y335_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0335</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0335</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64341">
        <property type="entry name" value="G64341"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869833.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57781"/>
    <dbReference type="SMR" id="Q57781"/>
    <dbReference type="STRING" id="243232.MJ_0335"/>
    <dbReference type="EnsemblBacteria" id="AAB98323">
        <property type="protein sequence ID" value="AAB98323"/>
        <property type="gene ID" value="MJ_0335"/>
    </dbReference>
    <dbReference type="GeneID" id="1451191"/>
    <dbReference type="KEGG" id="mja:MJ_0335"/>
    <dbReference type="eggNOG" id="arCOG09508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1122625_0_0_2"/>
    <dbReference type="OrthoDB" id="125019at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106805" description="Uncharacterized protein MJ0335">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="247" mass="27881" checksum="9972F8BC3FA792DF" modified="1996-11-01" version="1">MQSIFLPVLNPTTIDGQEITEEWIKKYGPTLRGKPVNIDHNYYSNGNLAVGDVVDVYFNPEGNLYAHIRIFDEIYWRLVDNGIKIKGVSFEFNDDGVGEEGIMKGLALCLESDPKVDFARLVEGNYVLEVLASIKRDSMDTKTQEKTEPKKIKDMTEEEFEKFLHEKIEQILASHKKENEDKDKSDNEDDKVVEILASKMDELVAVNKSVLKQLEEIRKAQKEILASAPVPPSGSGNSGHRRANLGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q57786</accession>
    <name>Y340_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0340</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0340</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64342">
        <property type="entry name" value="D64342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869838.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57786"/>
    <dbReference type="STRING" id="243232.MJ_0340"/>
    <dbReference type="EnsemblBacteria" id="AAB98328">
        <property type="protein sequence ID" value="AAB98328"/>
        <property type="gene ID" value="MJ_0340"/>
    </dbReference>
    <dbReference type="GeneID" id="1451196"/>
    <dbReference type="KEGG" id="mja:MJ_0340"/>
    <dbReference type="eggNOG" id="arCOG09645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182899_0_0_2"/>
    <dbReference type="OMA" id="YKNGASK"/>
    <dbReference type="OrthoDB" id="112710at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106810" description="Uncharacterized protein MJ0340">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <sequence length="234" mass="25463" checksum="4A9F5966C1F03056" modified="1996-11-01" version="1">MVLSLTPPSQENILASGKLAYFGAKSQVDTFTGDEQNDTFELTKDDAVLGSEIVKVNGVLQFEGQDYTAIHENGILKKIKFTQPPANGASITVEYLYLDLPLGGASELSVKEDKDKEELTVDCSYGKIQIEKGSSITLSFKDIITVGDIKLTAYFSGEILEGNTYSKYKNGASKSANIVVLAFSKEATMAYGVEPPKRIVIIRGAMPNNLEFDWSGGSKSFDLTAQSYEIIDVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57791</accession>
    <name>Y345_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0345</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0345</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64343">
        <property type="entry name" value="A64343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869843.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57791"/>
    <dbReference type="STRING" id="243232.MJ_0345"/>
    <dbReference type="EnsemblBacteria" id="AAB98335">
        <property type="protein sequence ID" value="AAB98335"/>
        <property type="gene ID" value="MJ_0345"/>
    </dbReference>
    <dbReference type="GeneID" id="1451201"/>
    <dbReference type="KEGG" id="mja:MJ_0345"/>
    <dbReference type="eggNOG" id="arCOG09649">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876063_0_0_2"/>
    <dbReference type="OMA" id="NVICEIT"/>
    <dbReference type="OrthoDB" id="124130at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106815" description="Uncharacterized protein MJ0345">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="317" mass="35379" checksum="9234BE19C4BA5533" modified="1996-11-01" version="1">MDCVTNATTRCRKGSIVVIVMPPLISNNTFFLMNTTNDGNGGSVSYDYPLLSNQNSLITNTTNNLTSDSTNTDISNETPILSNNTPILMNTPTNPNSDSDTTTPTVVSHTVELITEEKKVISKIVYSDGTEDVKELDLTDFYNSAYNEGYSAGVNSVDFSKTGYIIENGIIKVTTTLTNGKSQEYNITLDENLVVSLYNQIMANKSSCFQIRQNETEYSFLVESRQYQPQPNLAYQPAVDGTPVYEVLGQEQKTWSFTLYVDSIPKMQFLQQLASNPLIEVYFDEIGDWKQALIQSISLSRITTGHYYADIELIILE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q57809</accession>
    <name>Y363_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized MCM-type protein MJ0363</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0363</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64345">
        <property type="entry name" value="C64345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869862.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57809"/>
    <dbReference type="SMR" id="Q57809"/>
    <dbReference type="STRING" id="243232.MJ_0363"/>
    <dbReference type="EnsemblBacteria" id="AAB98345">
        <property type="protein sequence ID" value="AAB98345"/>
        <property type="gene ID" value="MJ_0363"/>
    </dbReference>
    <dbReference type="GeneID" id="1451220"/>
    <dbReference type="KEGG" id="mja:MJ_0363"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_2"/>
    <dbReference type="InParanoid" id="Q57809"/>
    <dbReference type="OMA" id="QADWDND"/>
    <dbReference type="OrthoDB" id="3067at2157"/>
    <dbReference type="PhylomeDB" id="Q57809"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
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    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000194129" description="Uncharacterized MCM-type protein MJ0363">
        <location>
            <begin position="1"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="domain" description="MCM">
        <location>
            <begin position="352"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="397"/>
            <end position="404"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="759" mass="86167" checksum="12240104136FAA05" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q57816</accession>
    <name>FTSZ1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell division protein FtsZ 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftsZ1</name>
        <name type="ordered locus">MJ0370</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Nature" volume="391" first="203" last="206">
            <title>Crystal structure of the bacterial cell-division protein ftsZ.</title>
            <authorList>
                <person name="Loewe J."/>
                <person name="Amos L.A."/>
            </authorList>
            <dbReference type="PubMed" id="9428770"/>
            <dbReference type="DOI" id="10.1038/34472"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH GDP</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1998" name="J. Struct. Biol." volume="124" first="235" last="243">
            <title>Crystal structure determination of FtsZ from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Loewe J."/>
            </authorList>
            <dbReference type="PubMed" id="10049809"/>
            <dbReference type="DOI" id="10.1006/jsbi.1998.4041"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 23-356</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="Nat. Struct. Mol. Biol." volume="11" first="1243" last="1250">
            <title>Structural insights into FtsZ protofilament formation.</title>
            <authorList>
                <person name="Oliva M.A."/>
                <person name="Cordell S.C."/>
                <person name="Lowe J."/>
            </authorList>
            <dbReference type="PubMed" id="15558053"/>
            <dbReference type="DOI" id="10.1038/nsmb855"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH GTP</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2007" name="J. Mol. Biol." volume="373" first="1229" last="1242">
            <title>Structural insights into the conformational variability of FtsZ.</title>
            <authorList>
                <person name="Oliva M.A."/>
                <person name="Trambaiolo D."/>
                <person name="Lowe J."/>
            </authorList>
            <dbReference type="PubMed" id="17900614"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2007.08.056"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEX WITH GDP</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer (PubMed:15558053). Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsZ family.</text>
    </comment>
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        <property type="resolution" value="3.00 A"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=1-364"/>
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    <dbReference type="PDBsum" id="1FSZ"/>
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    <dbReference type="EnsemblBacteria" id="AAB98359">
        <property type="protein sequence ID" value="AAB98359"/>
        <property type="gene ID" value="MJ_0370"/>
    </dbReference>
    <dbReference type="GeneID" id="1451227"/>
    <dbReference type="KEGG" id="mja:MJ_0370"/>
    <dbReference type="eggNOG" id="arCOG02201">
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    <dbReference type="OMA" id="GNPSIGQ"/>
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        <property type="organism ID" value="3260"/>
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    <dbReference type="EvolutionaryTrace" id="Q57816"/>
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        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
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    </dbReference>
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    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
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        </location>
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            <end position="48"/>
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            <begin position="153"/>
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        <location>
            <begin position="163"/>
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    <feature type="helix" evidence="8">
        <location>
            <begin position="167"/>
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    <feature type="strand" evidence="8">
        <location>
            <begin position="185"/>
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            <begin position="192"/>
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    <feature type="helix" evidence="8">
        <location>
            <begin position="195"/>
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        </location>
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    <feature type="strand" evidence="5">
        <location>
            <begin position="199"/>
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        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="204"/>
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        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="236"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="247"/>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="258"/>
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        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="279"/>
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        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="284"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="316"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="326"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="331"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="341"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="344"/>
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        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="350"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15558053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17900614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="9428770"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1W58"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1W59"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="1W5B"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2VAP"/>
        </source>
    </evidence>
    <sequence length="364" mass="38924" checksum="3BB386A5D2FCA107" modified="1996-11-01" version="1">MKFLKNVLEEGSKLEEFNELELSPEDKELLEYLQQTKAKITVVGCGGAGNNTITRLKMEGIEGAKTVAINTDAQQLIRTKADKKILIGKKLTRGLGAGGNPKIGEEAAKESAEEIKAAIQDSDMVFITCGLGGGTGTGSAPVVAEISKKIGALTVAVVTLPFVMEGKVRMKNAMEGLERLKQHTDTLVVIPNEKLFEIVPNMPLKLAFKVADEVLINAVKGLVELITKDGLINVDFADVKAVMNNGGLAMIGIGESDSEKRAKEAVSMALNSPLLDVDIDGATGALIHVMGPEDLTLEEAREVVATVSSRLDPNATIIWGATIDENLENTVRVLLVITGVQSRIEFTDTGLKRKKLELTGIPKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57824</accession>
    <name>CSA3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR locus-related putative DNA-binding protein Csa3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">csa3</name>
        <name type="ordered locus">MJ0379</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csa3 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64347">
        <property type="entry name" value="C64347"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57824"/>
    <dbReference type="SMR" id="Q57824"/>
    <dbReference type="STRING" id="243232.MJ_0379"/>
    <dbReference type="EnsemblBacteria" id="AAB98369">
        <property type="protein sequence ID" value="AAB98369"/>
        <property type="gene ID" value="MJ_0379"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0379"/>
    <dbReference type="eggNOG" id="arCOG01446">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1248328_0_0_2"/>
    <dbReference type="InParanoid" id="Q57824"/>
    <dbReference type="OMA" id="GEHINHI"/>
    <dbReference type="PhylomeDB" id="Q57824"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010163">
        <property type="entry name" value="Csa3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01884">
        <property type="entry name" value="cas_HTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106842" description="CRISPR locus-related putative DNA-binding protein Csa3">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="208" mass="24080" checksum="163D155C47FA2623" modified="1996-11-01" version="1">MVRYMRYIATFGYHTNHIFDKNGKIIGIDDEKISNMILIYSLDVDADENTVNSIKNTKNYIESKLKEYNIPYLFVEVNPYEFNTNVKNFRKYIVPKTIINLTGGKRIVGYALFYAAVLEKENVEKVFYVSKLGDIIEFPLIPPDIKLTELEMKILNLLDKEGEMSVSNIAHKLERSLSTISEYVSQLEKKGLVKKLSKGRRKIVKKVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q57832</accession>
    <name>RPO11_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit L</fullName>
            <shortName evidence="3">mjL</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo11</name>
        <name evidence="1" type="synonym">rpoL</name>
        <name type="ordered locus">MJ0387</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Nucleic Acids Res." volume="28" first="4299" last="4305">
            <title>Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12.</title>
            <authorList>
                <person name="Werner F."/>
                <person name="Eloranta J.J."/>
                <person name="Weinzierl R.O."/>
            </authorList>
            <dbReference type="PubMed" id="11058130"/>
            <dbReference type="DOI" id="10.1093/nar/28.21.4299"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the RNA polymerase complex (By similarity). Forms an Rpo3-Rpo10-Rpo11-Rpo12 complex upon coexpression (PubMed:11058130).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-2567038">
            <id>Q57832</id>
        </interactant>
        <interactant intactId="EBI-2567023">
            <id>Q57648</id>
            <label>rpo3</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64348">
        <property type="entry name" value="C64348"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869886.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57832"/>
    <dbReference type="SMR" id="Q57832"/>
    <dbReference type="IntAct" id="Q57832">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0387"/>
    <dbReference type="EnsemblBacteria" id="AAB98373">
        <property type="protein sequence ID" value="AAB98373"/>
        <property type="gene ID" value="MJ_0387"/>
    </dbReference>
    <dbReference type="GeneID" id="1451244"/>
    <dbReference type="KEGG" id="mja:MJ_0387"/>
    <dbReference type="eggNOG" id="arCOG04111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090381_5_0_2"/>
    <dbReference type="InParanoid" id="Q57832"/>
    <dbReference type="OMA" id="SYDMKHV"/>
    <dbReference type="OrthoDB" id="129321at2157"/>
    <dbReference type="PhylomeDB" id="Q57832"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000149326" description="DNA-directed RNA polymerase subunit Rpo11">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <sequence length="99" mass="11392" checksum="0C25307DDF5EE909" modified="1997-11-01" version="1">MEIKILERKDNLVEIELINEDHSLPNLLKDILLTKEGVKMASYSIDHPLLHPETGRYISNPKITIITEEGTDPLEVLKEGLRDIIKMCDTLLDELKEKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q57837</accession>
    <name>Y392_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Zinc metalloprotease MJ0392</fullName>
            <ecNumber>3.4.24.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>MjS2P</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>S2P endopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Site-2-type intramembrane protease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0392</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="Science" volume="318" first="1608" last="1612">
            <title>Structure of a site-2 protease family intramembrane metalloprotease.</title>
            <authorList>
                <person name="Feng L."/>
                <person name="Yan H."/>
                <person name="Wu Z."/>
                <person name="Yan N."/>
                <person name="Wang Z."/>
                <person name="Jeffrey P.D."/>
                <person name="Shi Y."/>
            </authorList>
            <dbReference type="PubMed" id="18063795"/>
            <dbReference type="DOI" id="10.1126/science.1150755"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 1-224</scope>
        <scope>FUNCTION AS A PROTEASE</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>ACTIVE SITE</scope>
        <scope>ZINC-BINDING SITES</scope>
    </reference>
    <comment type="function">
        <text evidence="4">A site-2 regulated intramembrane protease (S2P) that cleaves type-2 transmembrane proteins within their membrane-spanning domains; its endogenous substrate is unknown. Regulated intramembrane proteolysis (RIP) occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. A membrane-spanning regulatory substrate protein is first cut extracytoplasmically (site-1 protease, S1P), then within the membrane itself (site-2 protease, S2P, this enzyme), while cytoplasmic proteases finish degrading the regulatory protein, liberating the effector protein. Possible signals, S1P and substrates are unknown in this organism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Inhibited by 1,10-phenanthroline.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64348">
        <property type="entry name" value="H64348"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869891.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3B4R">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.30 A"/>
        <property type="chains" value="A/B=1-224"/>
    </dbReference>
    <dbReference type="PDBsum" id="3B4R"/>
    <dbReference type="AlphaFoldDB" id="Q57837"/>
    <dbReference type="SMR" id="Q57837"/>
    <dbReference type="STRING" id="243232.MJ_0392"/>
    <dbReference type="MEROPS" id="M50.006"/>
    <dbReference type="EnsemblBacteria" id="AAB98382">
        <property type="protein sequence ID" value="AAB98382"/>
        <property type="gene ID" value="MJ_0392"/>
    </dbReference>
    <dbReference type="GeneID" id="1451249"/>
    <dbReference type="KEGG" id="mja:MJ_0392"/>
    <dbReference type="eggNOG" id="arCOG00607">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037123_1_1_2"/>
    <dbReference type="InParanoid" id="Q57837"/>
    <dbReference type="OMA" id="IELHITF"/>
    <dbReference type="OrthoDB" id="51079at2157"/>
    <dbReference type="PhylomeDB" id="Q57837"/>
    <dbReference type="BRENDA" id="3.4.24.85">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57837"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016483">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006404">
        <property type="entry name" value="UCP006404_Pept_M50_CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00142">
        <property type="entry name" value="ZINC_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000088480" description="Zinc metalloprotease MJ0392">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="2">
        <location>
            <begin position="226"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="2">
        <location>
            <begin position="281"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="4"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="19"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="91"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="142"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="148"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="191"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="18063795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="18063795"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3B4R"/>
        </source>
    </evidence>
    <sequence length="339" mass="37965" checksum="A56EB35F186A6457" modified="1996-11-01" version="1">MNYSIRLFKIMGIPIELHITFILFLVVIIGLSIMNNSIFWAVLFILLFVSVVLHELGHSYVAKKYGVKIEKILLLPIGGVAMMDKIPKEGELRIGIAGPLVSFIIGIVLLIVSQFFDININGYPLLYTLSLLNLMLGGFNLIPAFPMDGGRILRAILSKKYGYLKSTKIAANIGKSLALIMLLFGLLSMNIILILVSLFVYFGAEQESRVVEVETIFKNIKAKDIMTPNPVYVTPDMSIEEFLDFMLKHKYFGYPVVENGKLVGCIGIGNIHKKEGTVRDYMEKPVVVSEDTDIKEILRKMANTDRVFVVEGGKLKGIISKTDILRAMSILELKEELKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q57873</accession>
    <name>Y431_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0333 protein MJ0431</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0431</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0333 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64353">
        <property type="entry name" value="G64353"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57873"/>
    <dbReference type="SMR" id="Q57873"/>
    <dbReference type="EnsemblBacteria" id="AAB98432">
        <property type="protein sequence ID" value="AAB98432"/>
        <property type="gene ID" value="MJ_0431"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0431"/>
    <dbReference type="eggNOG" id="arCOG11030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197251_0_0_2"/>
    <dbReference type="OMA" id="NVKYSDT"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000218265" description="UPF0333 protein MJ0431">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="75" mass="7890" checksum="A48FB475CFD9F629" modified="1996-11-01" version="1">MGKMKILKKLLSKKGQLSMEVGVLVAAAVLVAIIAAYFYVKNAKSAVASAGNKSAAFINVTANKSQEYISNLSNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57896</accession>
    <name>MTNA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="1">M1Pi</shortName>
            <shortName evidence="1">MTR-1-P isomerase</shortName>
            <ecNumber evidence="1">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">MTNA-like protein</fullName>
            <shortName evidence="1">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0454</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64356">
        <property type="entry name" value="F64356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869953.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57896"/>
    <dbReference type="SMR" id="Q57896"/>
    <dbReference type="STRING" id="243232.MJ_0454"/>
    <dbReference type="PRIDE" id="Q57896"/>
    <dbReference type="EnsemblBacteria" id="AAB98443">
        <property type="protein sequence ID" value="AAB98443"/>
        <property type="gene ID" value="MJ_0454"/>
    </dbReference>
    <dbReference type="GeneID" id="1451315"/>
    <dbReference type="KEGG" id="mja:MJ_0454"/>
    <dbReference type="eggNOG" id="arCOG01123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_1_2_2"/>
    <dbReference type="InParanoid" id="Q57896"/>
    <dbReference type="OMA" id="RLWVDET"/>
    <dbReference type="OrthoDB" id="54988at2157"/>
    <dbReference type="PhylomeDB" id="Q57896"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019284">
        <property type="term" value="P:L-methionine salvage from S-adenosylmethionine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156086" description="Putative methylthioribose-1-phosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="233"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="329" mass="36803" checksum="3936298C574730C9" modified="1997-11-01" version="1">MTKDLRPIIWDDDKKELILIDQRKLPNKLEYFICKTYEDVAYAIKDMVVRGAPAIGVSAAYGLALAEINGDDIYKAYEVLKNTRPTAVNLFWALDRCLTAYKEGKSILDEAKKIHEEDIETCKKIGMIGEKLIEDGDTILTHCNAGALATSAYGTALSVIRFAFYNGKKIRVIADETRPRLQGAKLTAFELNYEGIPVKVITDNTAGFLMQKGEIDKIIVGADRILADGTVYNKIGTYSLAVLAKYHRIPFYVAAPLSTFDLRSSEEDVIIEERDEKEVAYIDGVRIVPEGVGCYNYAFDKTPPDLITAIITEKGIVKPNRDEILKLFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57898</accession>
    <name>Y456_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter MJ0456</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0456</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CitM (TC 2.A.11) transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64356">
        <property type="entry name" value="H64356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869955.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57898"/>
    <dbReference type="SMR" id="Q57898"/>
    <dbReference type="STRING" id="243232.MJ_0456"/>
    <dbReference type="EnsemblBacteria" id="AAB98444">
        <property type="protein sequence ID" value="AAB98444"/>
        <property type="gene ID" value="MJ_0456"/>
    </dbReference>
    <dbReference type="GeneID" id="1451317"/>
    <dbReference type="KEGG" id="mja:MJ_0456"/>
    <dbReference type="eggNOG" id="arCOG00237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063025_1_0_2"/>
    <dbReference type="InParanoid" id="Q57898"/>
    <dbReference type="OMA" id="ICSMFVT"/>
    <dbReference type="OrthoDB" id="49249at2157"/>
    <dbReference type="PhylomeDB" id="Q57898"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000106885" description="Uncharacterized transporter MJ0456">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="352" mass="40636" checksum="A18197DC93B24412" modified="1996-11-01" version="1">MKIDTFIFLMFISIGILLLLLNIINPMEVFHIVEWKTIFSLFYLMVIINIMRDTKFLDYISLKILKKSKRIFIALIFLTLFLSSLITNDVSLFVIIPLTLIIHRYTNMPFKDLEKLIIFEGVSANIGSGLTPIGNPQNLFLFHFYNIGTLEFIINMIPFEIFGILAILPFLEFKKYDTKINIDIKFKKEWIFYILSFILVLLCVFGYLNFIYILPLILAILMYKRVKVDYLFLLTFIFLFVDIEGLKRIGIINIFSIKCGNVMLMIYASLLSQIISNVPATVLLSHLYKNWLPIAYGVNIGGNGTLIASFANLITLRLSNGNVRVGRFLLIGGIIYIMHLIVLVAYAKIFWV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57913</accession>
    <name>Y489_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0489</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0489</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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    <dbReference type="PDBsum" id="5D5O"/>
    <dbReference type="PDBsum" id="5D5T"/>
    <dbReference type="AlphaFoldDB" id="Q57913"/>
    <dbReference type="SMR" id="Q57913"/>
    <dbReference type="STRING" id="243232.MJ_0489"/>
    <dbReference type="EnsemblBacteria" id="AAB98480">
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    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0489"/>
    <dbReference type="eggNOG" id="arCOG04864">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="InterPro" id="IPR009573">
        <property type="entry name" value="HcgC"/>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106895" description="Uncharacterized protein MJ0489">
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            <begin position="1"/>
            <end position="268"/>
        </location>
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            <begin position="15"/>
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    <feature type="strand" evidence="1">
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    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="5D4V"/>
        </source>
    </evidence>
    <sequence length="268" mass="30532" checksum="1A24C6D66FA955A6" modified="1996-11-01" version="1">MGCGIMKYGITEMVKTIDTKTRVVDVTNEIAKKKYQAIRDFLEGEEFKEVVIFGVYLWGNYTAQMLSKYADKVYLVDIHEFMKGFVPNNNSIKFLNLNEFKLKFIRGEVNPDLIVDLTGLGGIEPEFLAKFNPKVFIVEDPKGVFDVDIYEADNTYKRTAPFIEKAKVGVLKTYRKARVSKTSGTMTLTIDTIVDASREITSLDGVLYAIPNLRYYEGILFHENDIHKFLSEISQPAITISTLNDVLDEAEEILSNNINLIYSFVEEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57916</accession>
    <name>NADC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber>2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
            <shortName>QAPRTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">nadC</name>
        <name type="ordered locus">MJ0493</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>CO2 + diphosphate + nicotinate beta-D-ribonucleotide = 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+) + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NadC/ModD family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    </dbReference>
    <dbReference type="GeneID" id="1451355"/>
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    <dbReference type="HOGENOM" id="CLU_039622_2_0_2"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0009435">
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    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="CDD" id="cd01572">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="NadC"/>
    </dbReference>
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        <property type="entry name" value="NadC/ModD"/>
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    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="PTHR32179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="SSF51690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155952" description="Probable nicotinate-nucleotide pyrophosphorylase [carboxylating]">
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            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="128"/>
            <end position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
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        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
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        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
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        <ligand>
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        </ligand>
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        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="263"/>
            <end position="265"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="283" mass="31970" checksum="BDD118E9CE1401A8" modified="1996-11-01" version="1">MLKDYALKILKKSLEYDVGFGDITTNSIIPEGVKAKGVIKAKEKCIVCGIDFIVAFFEEYGIKCKKLFNDGEEAYGNILEFEGDARTILMLERTALNLLMHLSGIATMTNRIVKKAKSVNKNVRVACTRKTLPLLSPLQKYAVYIGGGDTHRFRLDDCVLIKDNHIAIVGVKEAIRRAKENVSFTKKIEVEVSNLKELREALEERADIIMLDNFKPEEIEEALKIIDEFERKTNFKPIIEVSGGIKEDNILEYAKYNVDVISMGALTHSVKSVDMSLDIVRYQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q57931</accession>
    <name>TYSY_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative thymidylate synthase</fullName>
            <shortName evidence="1">TS</shortName>
            <shortName evidence="1">TSase</shortName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thyA</name>
        <name type="ordered locus">MJ0511</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May catalyze the biosynthesis of dTMP using an unknown cosubstrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidylate synthase family. Archaeal-type ThyA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64363">
        <property type="entry name" value="G64363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870012.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57931"/>
    <dbReference type="SMR" id="Q57931"/>
    <dbReference type="STRING" id="243232.MJ_0511"/>
    <dbReference type="EnsemblBacteria" id="AAB98502">
        <property type="protein sequence ID" value="AAB98502"/>
        <property type="gene ID" value="MJ_0511"/>
    </dbReference>
    <dbReference type="GeneID" id="1451373"/>
    <dbReference type="KEGG" id="mja:MJ_0511"/>
    <dbReference type="eggNOG" id="arCOG03214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084975_0_0_2"/>
    <dbReference type="InParanoid" id="Q57931"/>
    <dbReference type="OMA" id="HHSVSMH"/>
    <dbReference type="OrthoDB" id="57461at2157"/>
    <dbReference type="PhylomeDB" id="Q57931"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01686">
        <property type="entry name" value="Thymidy_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014620">
        <property type="entry name" value="Thymidylate_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020940">
        <property type="entry name" value="Thymidylate_synthase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="PTHR11548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036752">
        <property type="entry name" value="TSase_MJ051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="SSF55831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03283">
        <property type="entry name" value="thy_syn_methano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00091">
        <property type="entry name" value="THYMIDYLATE_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141055" description="Putative thymidylate synthase">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01686"/>
        </source>
    </evidence>
    <sequence length="222" mass="25933" checksum="3BC5E8C38237CE92" modified="1996-11-01" version="1">MLCIKKPSVASAFNELIPKILKDGEVVETEFEERTKEIRNTIIEITNPKLKKVPEKYPLGEKAVEEYTKNLLYGSKNVFSYDYHQRLFEYPYADEKINQIDYIIEKLNQQKNSRRAVAITWNPKIDIEVSRDERGSVPCLQLVQFLIRNGKLYQTVIFRSNDALLAFVSNAIGLITLGEYIAKKVGVGYGTYTHHAISMHIYVDRDFDYIKKYFPECLKYLW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q57989</accession>
    <name>HEM3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable porphobilinogen deaminase</fullName>
            <shortName>PBG</shortName>
            <ecNumber>2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Hydroxymethylbilane synthase</fullName>
            <shortName>HMBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hemC</name>
        <name type="ordered locus">MJ0569</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>H2O + 4 porphobilinogen = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
        <text evidence="1">Binds 1 dipyrromethane group covalently.</text>
    </comment>
    <comment type="pathway">
        <text>Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The porphobilinogen subunits are added to the dipyrromethane group.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64371">
        <property type="entry name" value="A64371"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57989"/>
    <dbReference type="SMR" id="Q57989"/>
    <dbReference type="STRING" id="243232.MJ_0569"/>
    <dbReference type="EnsemblBacteria" id="AAB98563">
        <property type="protein sequence ID" value="AAB98563"/>
        <property type="gene ID" value="MJ_0569"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0569"/>
    <dbReference type="eggNOG" id="arCOG04299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_1_0_2"/>
    <dbReference type="InParanoid" id="Q57989"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="PhylomeDB" id="Q57989"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018160">
        <property type="term" value="P:peptidyl-pyrromethane cofactor linkage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PTHR11557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="SSF54782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000143024" description="Probable porphobilinogen deaminase">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="modified residue" description="S-(dipyrrolylmethanemethyl)cysteine" evidence="1">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="292" mass="32783" checksum="F8E9823E484D2C06" modified="1997-11-01" version="1">MIRIGTRGSKLALYQANKVAELLKNLGYKVEIKIIKTTGDRVLDKKLSDIGIGVFTKELDLAMLNNEIDIAVHSLKDIPTIWNENLMVGAVLERDSYHDLLIWNKDIDFNEDSKIVIGTSSMRRRAFLKFIYPNAKFELLRGNVDTRLRKLKEGLYDAIVLSEAGIIRLGVSLEDFNYKRLDILPAPAQGIIAVACKRDDEEMKSILKEINHERTYLESLCERTALNEFGGGCSVPFGALAVYDEKNELLKLKAAVVTNDELKNASGEVKCKIDEIDKAVELGKKIGLKLKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58004</accession>
    <name>Y584_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0718 protein MJ0584</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0584</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0718 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64372">
        <property type="entry name" value="H64372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58004"/>
    <dbReference type="STRING" id="243232.MJ_0584"/>
    <dbReference type="EnsemblBacteria" id="AAB98577">
        <property type="protein sequence ID" value="AAB98577"/>
        <property type="gene ID" value="MJ_0584"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0584"/>
    <dbReference type="eggNOG" id="arCOG02712">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058185_0_0_2"/>
    <dbReference type="InParanoid" id="Q58004"/>
    <dbReference type="OMA" id="TVMAFMM"/>
    <dbReference type="PhylomeDB" id="Q58004"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005524">
        <property type="entry name" value="DUF318"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03773">
        <property type="entry name" value="ArsP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106944" description="UPF0718 protein MJ0584">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="344"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="365" mass="39663" checksum="FB7C58364885C699" modified="1998-12-15" version="3">MKMDVMSFIMNIINVMINTIIDYLNVNRVLALLMAFLMAGGIASMINKNFIIKYFGSNTPKYISYTVAAVSGSLLAVCSCTILPLFASIYKRGAGIGPATTFLFSGPAINVLAIFYSAALLGWDIGFLRAVFAVVVSILIGLSMEIIFKSHEKKRALRVPKADKISDRPLYQTITFFALQFIMLLVITASPKLFPTLSMPLYDGFLLKHLLFIILGIILAVTTKIWFKDEEIKNWLRESFTLLKIVFPLLIIGVAIAGAIKAIIPPSYIATYVGGNSITANFIASFIGALMYFATLTEVPIIKALMELGMGVGPAMALLLAGPSLSIPTVLTISKVLGKTKALTYLGLVVIFSTICGYIAGIILR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58011</accession>
    <name>SDO1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Ribosome maturation protein SDO1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0592</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64373">
        <property type="entry name" value="H64373"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58011"/>
    <dbReference type="SMR" id="Q58011"/>
    <dbReference type="STRING" id="243232.MJ_0592"/>
    <dbReference type="EnsemblBacteria" id="AAB98586">
        <property type="protein sequence ID" value="AAB98586"/>
        <property type="gene ID" value="MJ_0592"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0592"/>
    <dbReference type="eggNOG" id="arCOG04187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043216_2_0_2"/>
    <dbReference type="InParanoid" id="Q58011"/>
    <dbReference type="OMA" id="AVNPQMD"/>
    <dbReference type="PhylomeDB" id="Q58011"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:mature ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018023">
        <property type="entry name" value="Ribosome_mat_SBDS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="Ribosome_mat_Sdo1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="Ribosome_mat_Sdo1/SBDS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="PTHR10927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="SSF109728"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="SSF89895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01267">
        <property type="entry name" value="UPF0023"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000123769" description="Ribosome maturation protein SDO1 homolog">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="240" mass="27411" checksum="6517AF7EDBBA2F64" modified="1996-11-01" version="1">MGRDIMVSLEEAVIARYTSHGEKFEILVDPYLAAKLKEGQNVDFDELLAIEVVFRDASKGEKAPEELLSKIFGTTDVKEIAKKIILKGQVQLTAKQREEIREQKKRQIITIISRNTINPQTDTPHPPHRIEKAMEELRINIDIYKSAEEQVPEIVKKLKKVLPIRFEKRDIAVKIPAEFASKAYNALYQFGAVKQEEWQPDGSLIVLIEIPSGIEAEFYAHLNKITKGNVQTKVVKKYSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58013</accession>
    <name>Y596_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0596</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0596</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64374">
        <property type="entry name" value="D64374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870100.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58013"/>
    <dbReference type="SMR" id="Q58013"/>
    <dbReference type="STRING" id="243232.MJ_0596"/>
    <dbReference type="EnsemblBacteria" id="AAB98596">
        <property type="protein sequence ID" value="AAB98596"/>
        <property type="gene ID" value="MJ_0596"/>
    </dbReference>
    <dbReference type="GeneID" id="1451461"/>
    <dbReference type="KEGG" id="mja:MJ_0596"/>
    <dbReference type="eggNOG" id="arCOG03415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1860681_0_0_2"/>
    <dbReference type="OMA" id="KWFVVKK"/>
    <dbReference type="OrthoDB" id="94150at2157"/>
    <dbReference type="PhylomeDB" id="Q58013"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022620">
        <property type="entry name" value="DUF2666"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10869">
        <property type="entry name" value="DUF2666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106949" description="Uncharacterized protein MJ0596">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <sequence length="141" mass="16433" checksum="4E2A7CC57ACBEF3D" modified="1996-11-01" version="1">MEDKIEFMAKHKKWFVVKKLKIDENTEDIEIARLLASIDETVLNKIPEYLPFDMNKLYEIADGIYQKKKGRITEEEIAEVLKKLKSPATTRKLNEITESKEGKEILKAILNNIILERLGIQTRVSPKVIEKYIENSQSSNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58021</accession>
    <name>Y604_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ0604</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ0604</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0604</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Putative antitoxin component of a putative type VII toxin-antitoxin (TA) system. Its cognate toxin might be MJ0605, which it might AMPylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64375">
        <property type="entry name" value="D64375"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870108.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58021"/>
    <dbReference type="SMR" id="Q58021"/>
    <dbReference type="STRING" id="243232.MJ_0604"/>
    <dbReference type="EnsemblBacteria" id="AAB98594">
        <property type="protein sequence ID" value="AAB98594"/>
        <property type="gene ID" value="MJ_0604"/>
    </dbReference>
    <dbReference type="GeneID" id="1451469"/>
    <dbReference type="KEGG" id="mja:MJ_0604"/>
    <dbReference type="eggNOG" id="arCOG01195">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_3_2_2"/>
    <dbReference type="InParanoid" id="Q58021"/>
    <dbReference type="OMA" id="INWSFHR"/>
    <dbReference type="OrthoDB" id="108083at2157"/>
    <dbReference type="PhylomeDB" id="Q58021"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106953" description="Putative protein adenylyltransferase MJ0604">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="100" mass="11667" checksum="A259493170FF0BC2" modified="1997-11-01" version="1">MNEEKAIKEFVNALKSKYRGRIKKIILFGSYARGDYTEESDIDILIVGDVDFDYVIDLCTKLLLKYGVVINAIVESEELFNKKINWSFHRNVLEEGRVLY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58052</accession>
    <name>Y635_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0635</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0635</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64379">
        <property type="entry name" value="C64379"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870140.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58052"/>
    <dbReference type="SMR" id="Q58052"/>
    <dbReference type="STRING" id="243232.MJ_0635"/>
    <dbReference type="EnsemblBacteria" id="AAB98633">
        <property type="protein sequence ID" value="AAB98633"/>
        <property type="gene ID" value="MJ_0635"/>
    </dbReference>
    <dbReference type="GeneID" id="1451501"/>
    <dbReference type="KEGG" id="mja:MJ_0635"/>
    <dbReference type="eggNOG" id="arCOG08205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982156_0_0_2"/>
    <dbReference type="OMA" id="GFKIYTK"/>
    <dbReference type="OrthoDB" id="83357at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106963" description="Uncharacterized protein MJ0635">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <sequence length="283" mass="32502" checksum="1CE52FC8457C5E20" modified="1996-11-01" version="1">MKFMRIVRILAILLALIIGTSLCGCLNDSKSEADELIKMLPVDYNGFVYVNFKNIEDSKYSSEYRSKILNALRLGNANGEKTGIYINKTKRMIFSGSGYDRFVIIIEGDYDFDKFKNHLKEIGVNPVEEYGGFKIYTKPNDDKIALTFYKDMIIAGTKQGVYDCINVINGEMDSLLKNREVMEIYDRLPSDACVYEVSGTYSPWYKTVAEGMSISFENNDRVKVVRVEKYKDEETAKEKYEELLKKKDRDKEEMEKKGITADIKLDGQYLIVTVEGPEDELKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58054</accession>
    <name>PHEA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Prephenate dehydratase</fullName>
            <shortName>PDT</shortName>
            <ecNumber>4.2.1.51</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>MjPDT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pheA</name>
        <name type="ordered locus">MJ0637</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Biochemistry" volume="45" first="14101" last="14110">
            <title>A monofunctional and thermostable prephenate dehydratase from the archaeon Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Kleeb A.C."/>
                <person name="Kast P."/>
                <person name="Hilvert D."/>
            </authorList>
            <dbReference type="PubMed" id="17115705"/>
            <dbReference type="DOI" id="10.1021/bi061274n"/>
        </citation>
        <scope>BIOPHYSICAL CHARACTERIZATION</scope>
        <scope>SUBUNIT</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>INHIBITION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>H(+) + prephenate = 3-phenylpyruvate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:21648"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:18005"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="4.2.1.51"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text>Inhibited by L-phenylalanine but not by L-tyrosine or L-tryptophan.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM>22 uM for prephenate</KM>
        </kinetics>
        <phDependence>
            <text>Optimum pH is 5-10.</text>
        </phDependence>
        <temperatureDependence>
            <text>Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-phenylalanine biosynthesis; phenylpyruvate from prephenate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="mass spectrometry" mass="31413.0" method="Electrospray" evidence="4"/>
    <dbReference type="EC" id="4.2.1.51"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64379">
        <property type="entry name" value="E64379"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870142.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58054"/>
    <dbReference type="SMR" id="Q58054"/>
    <dbReference type="STRING" id="243232.MJ_0637"/>
    <dbReference type="PRIDE" id="Q58054"/>
    <dbReference type="EnsemblBacteria" id="AAB98631">
        <property type="protein sequence ID" value="AAB98631"/>
        <property type="gene ID" value="MJ_0637"/>
    </dbReference>
    <dbReference type="GeneID" id="1451503"/>
    <dbReference type="KEGG" id="mja:MJ_0637"/>
    <dbReference type="eggNOG" id="arCOG00255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035008_0_2_2"/>
    <dbReference type="InParanoid" id="Q58054"/>
    <dbReference type="OMA" id="PLMIYRE"/>
    <dbReference type="OrthoDB" id="47176at2157"/>
    <dbReference type="PhylomeDB" id="Q58054"/>
    <dbReference type="SABIO-RK" id="Q58054"/>
    <dbReference type="UniPathway" id="UPA00121">
        <property type="reaction ID" value="UER00345"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00857">
        <property type="entry name" value="PREPHENATE_DEHYDR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119180" description="Prephenate dehydratase">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate dehydratase" evidence="2">
        <location>
            <begin position="4"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="194"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="site" description="Essential for activity" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="17115705"/>
        </source>
    </evidence>
    <sequence length="272" mass="31415" checksum="0A5FDCE74AB7C3AD" modified="1996-11-01" version="1">MNKAVIYTLPKGTYSEKATKKFLDYIDGDYKIDYCNSIYDVFERVDNNGLGVVPIENSIEGSVSLTQDLLLQFKDIKILGELALDIHHNLIGYDKNKIKTVISHPQALAQCRNYIKKHGWDVKAVESTAKAVKIVAESKDETLGAIGSKESAEHYNLKILDENIEDYKNNKTRFILIGKKVKFKYHPKNYKVSIVFELKEDKPGALYHILKEFAERNINLTRIESRPSKKRLGTYIFYIDFENNKEKLEEILKSLERHTTFINLLGKYPVFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58060</accession>
    <name>Y644_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0644</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0644</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64380">
        <property type="entry name" value="D64380"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58060"/>
    <dbReference type="SMR" id="Q58060"/>
    <dbReference type="STRING" id="243232.MJ_0644"/>
    <dbReference type="EnsemblBacteria" id="AAB98638">
        <property type="protein sequence ID" value="AAB98638"/>
        <property type="gene ID" value="MJ_0644"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0644"/>
    <dbReference type="eggNOG" id="arCOG00117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072626_3_1_2"/>
    <dbReference type="InParanoid" id="Q58060"/>
    <dbReference type="OMA" id="VNVPVVC"/>
    <dbReference type="PhylomeDB" id="Q58060"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047443">
        <property type="term" value="F:4-hydroxy-4-methyl-2-oxoglutarate aldolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="SSF89562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106969" description="Uncharacterized protein MJ0644">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <sequence length="208" mass="22709" checksum="422F40333C23A9D5" modified="1996-11-01" version="1">MKVLLKIIVGRAMNILKNFSVPNLCDAGAKPLNGIKPILENQKLVFGEAITVKISYNDWGTLIKTISFAKNKFIVAEVVGEGKYETAVWGGLASLNAKIKGVRGVVIDGCVRDVEDIKALKFPVFAKNFCPNAGKPLNLGEINVAVNCCGVIVEPGDIIVGDCNGVVVIKKESLPEIIENAKNIKEKERKIRERILRGQDLRDVLNLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58067</accession>
    <name>Y651_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protease MJ0651</fullName>
            <ecNumber>3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0651</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S49 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870156.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58067"/>
    <dbReference type="SMR" id="Q58067"/>
    <dbReference type="STRING" id="243232.MJ_0651"/>
    <dbReference type="EnsemblBacteria" id="AAB98642">
        <property type="protein sequence ID" value="AAB98642"/>
        <property type="gene ID" value="MJ_0651"/>
    </dbReference>
    <dbReference type="GeneID" id="1451517"/>
    <dbReference type="KEGG" id="mja:MJ_0651"/>
    <dbReference type="eggNOG" id="arCOG01311">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046540_0_0_2"/>
    <dbReference type="InParanoid" id="Q58067"/>
    <dbReference type="OMA" id="YVYDEIR"/>
    <dbReference type="OrthoDB" id="36138at2157"/>
    <dbReference type="PhylomeDB" id="Q58067"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <feature type="chain" id="PRO_0000171456" description="Putative protease MJ0651">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="311" mass="34665" checksum="C46AAC8451216D64" modified="1998-12-15" version="2">MKKIYIILLILFVILISLIGASILLVMSLSGENVDLFGGEKIAKVYLCNEIYFDYNQGDGIFPQQKKDARYYINLLDDLEKDDSVKGVLLVVNSPGGEVIASEKLARKVEELAKKKPVVVYVEGLDASGAYMVSAPADYIVAEKHSIVGSIGVRMDLMHYYGLMKKLGINVTTIKAGKYKDIGSPFRPMTKEEKEYLQKMINETYMDFVKWVAEHRHLSINYTLKIADGKIYSGEDAKKVGLVDEVGTEEDALKKLEQLANVSNPEIVEYGLEENKGLFGLTYYLGYGIGKGIGEVLYGMEKINGRVELLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58100</accession>
    <name>Y687_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0687</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0687</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64385">
        <property type="entry name" value="G64385"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870192.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58100"/>
    <dbReference type="SMR" id="Q58100"/>
    <dbReference type="STRING" id="243232.MJ_0687"/>
    <dbReference type="EnsemblBacteria" id="AAB98682">
        <property type="protein sequence ID" value="AAB98682"/>
        <property type="gene ID" value="MJ_0687"/>
    </dbReference>
    <dbReference type="GeneID" id="1451553"/>
    <dbReference type="KEGG" id="mja:MJ_0687"/>
    <dbReference type="eggNOG" id="arCOG02470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040882_2_0_2"/>
    <dbReference type="InParanoid" id="Q58100"/>
    <dbReference type="OMA" id="NAMATKQ"/>
    <dbReference type="OrthoDB" id="28147at2157"/>
    <dbReference type="PhylomeDB" id="Q58100"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025377">
        <property type="entry name" value="DUF4367"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029046">
        <property type="entry name" value="LolA/LolB/LppX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004564">
        <property type="entry name" value="OM_lipoprot_carrier_LolA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14285">
        <property type="entry name" value="DUF4367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03548">
        <property type="entry name" value="LolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89392">
        <property type="entry name" value="SSF89392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106990" description="Uncharacterized protein MJ0687">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <sequence length="335" mass="38437" checksum="B42CF3FA368841C3" modified="1998-12-15" version="2">MNYKYLILSLFLIVGVFFAGCTQQMNADEIAKKMQEKYEAMKSMEADVLITTNIMGQTETMQYKYAFEKPNKFYMENDDVLIVCDGKTYYMYDKKKNQYTKMEIKGELNNMFNPDYGKFIKSMLEKFNVSYLGEKTYDGRKCYVLELISKENPEEKMKMYVDEEYWQPLKIEMDGVTIEYKNVKFNVDVPDDRFKFVPPEGAKLMSSGAMTTSKNIDEVQKDVSFKILVPKYTAGLELQNAMATKQNANNEESETVILTYGENGELAIIESKDNKPLTIPENGSNLITLKNGVKALISDSGDVKMLMFEYNGIKVIIAGKLDKNELIKIANSMIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q58154</accession>
    <name>HDRC1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CoB--CoM heterodisulfide reductase iron-sulfur subunit C 1</fullName>
            <ecNumber evidence="3">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrC1</name>
        <name type="ordered locus">MJ0744</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64392">
        <property type="entry name" value="H64392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870249.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58154"/>
    <dbReference type="SMR" id="Q58154"/>
    <dbReference type="STRING" id="243232.MJ_0744"/>
    <dbReference type="EnsemblBacteria" id="AAB98738">
        <property type="protein sequence ID" value="AAB98738"/>
        <property type="gene ID" value="MJ_0744"/>
    </dbReference>
    <dbReference type="GeneID" id="1451621"/>
    <dbReference type="KEGG" id="mja:MJ_0744"/>
    <dbReference type="eggNOG" id="arCOG00964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121273_0_0_2"/>
    <dbReference type="InParanoid" id="Q58154"/>
    <dbReference type="OMA" id="DPDLWLC"/>
    <dbReference type="OrthoDB" id="102216at2157"/>
    <dbReference type="PhylomeDB" id="Q58154"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017680">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03290">
        <property type="entry name" value="CoB_CoM_SS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000150073" description="CoB--CoM heterodisulfide reductase iron-sulfur subunit C 1">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="67"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LY39"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="194" mass="22016" checksum="8871167DFD0C83AD" modified="1996-11-01" version="1">MVIYAQKDISNDFIKEIIKTGEILGEGHVSSFKACYQCGTCTGSCPSGRITAFRTRKLIRYAQFGMKSAIIDSEDLWMCTTCYECYERCPRTVKITDIIKVLRNIAAREGKMAEAHKKTALYVFKTGHAVPINDQIKKARKEIGLTEIPPTTHKYPDALEVVRGIMKDLRFCDMVGICTETMQLKPVEWKDMSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58180</accession>
    <name>Y770_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0770</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0770</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'phage' integrase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64396">
        <property type="entry name" value="B64396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870275.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58180"/>
    <dbReference type="SMR" id="Q58180"/>
    <dbReference type="STRING" id="243232.MJ_0770"/>
    <dbReference type="EnsemblBacteria" id="AAB98775">
        <property type="protein sequence ID" value="AAB98775"/>
        <property type="gene ID" value="MJ_0770"/>
    </dbReference>
    <dbReference type="GeneID" id="1451647"/>
    <dbReference type="KEGG" id="mja:MJ_0770"/>
    <dbReference type="eggNOG" id="arCOG06578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472098_0_0_2"/>
    <dbReference type="InParanoid" id="Q58180"/>
    <dbReference type="OMA" id="HYGINTH"/>
    <dbReference type="OrthoDB" id="100194at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107025" description="Uncharacterized protein MJ0770">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="1">
        <location>
            <position position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01246"/>
        </source>
    </evidence>
    <sequence length="223" mass="26733" checksum="739C8CE50FF67B5B" modified="1996-11-01" version="1">MMIWDWNLSKPSESIKKHSGTWDKGIDYKQTYKMFKEDLQKLKNKELLYEDDYKRIAYLITFLFQLRNGCRIWEAIAGMINIAINIDNLNWNERITVKVRTQKRKDWEFRELIIPKCIKKEDIEMVRDVFLDIKKEIDEKLTMDEKLKAKKKIVKRFGAWLYKNYGINTHSLRYAYVTYLGEHGIPAQVLAKITKHKNINYIETYTQSRLAKEILKNIGDLDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58211</accession>
    <name>Y801_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0801</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0801</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64400">
        <property type="entry name" value="A64400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870311.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58211"/>
    <dbReference type="STRING" id="243232.MJ_0801"/>
    <dbReference type="EnsemblBacteria" id="AAB98799">
        <property type="protein sequence ID" value="AAB98799"/>
        <property type="gene ID" value="MJ_0801"/>
    </dbReference>
    <dbReference type="GeneID" id="1451683"/>
    <dbReference type="KEGG" id="mja:MJ_0801"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q58211"/>
    <dbReference type="OMA" id="EILEFML"/>
    <dbReference type="OrthoDB" id="36621at2157"/>
    <dbReference type="PhylomeDB" id="Q58211"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184671" description="Uncharacterized ATP-binding protein MJ0801">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="379" mass="44716" checksum="1BAF2567E0C5D0B4" modified="1997-11-01" version="1">MKFFDREREINEILHILNREPDDIYFIYGPLNSGKTALIKHIIENKLSDDYKVFYINFRTYLISEKREFIEAIFTTKKDDFFEKIKDKDEVLNLITKGVRILTGIPIPEVEFDKLFEEKINDAFQYLNSLLLEVKKSGKKPILIFDELQMIKDVVLNTENQRFSAFPSLRFGNGQKYLLKELFQFLVSLTKEQHLCHVFCLSSDSLFIEYVYSTGELEGRAKYLLVDDFDKETALKFMDFLAVENNINLTNEDKELIYSYVGGKPKDIKYVVEESKFKDLREILEFMLKDAVQKLDMFLDMLNYSKPKVDVGDEVIEIKKDNVIEALKLFKDEYEVSKKHIPVPVYTYLIKRNILFLNPIEGILKPQSYLVWNAIKRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58233</accession>
    <name>Y823_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0823</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0823</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0084 and MJ0685.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64402">
        <property type="entry name" value="G64402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870334.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58233"/>
    <dbReference type="SMR" id="Q58233"/>
    <dbReference type="STRING" id="243232.MJ_0823"/>
    <dbReference type="EnsemblBacteria" id="AAB98822">
        <property type="protein sequence ID" value="AAB98822"/>
        <property type="gene ID" value="MJ_0823"/>
    </dbReference>
    <dbReference type="GeneID" id="1451706"/>
    <dbReference type="KEGG" id="mja:MJ_0823"/>
    <dbReference type="eggNOG" id="arCOG00587">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082962_0_0_2"/>
    <dbReference type="InParanoid" id="Q58233"/>
    <dbReference type="OMA" id="GCVCPAS"/>
    <dbReference type="OrthoDB" id="61135at2157"/>
    <dbReference type="PhylomeDB" id="Q58233"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107063" description="Uncharacterized ATP-binding protein MJ0823">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="257" mass="28541" checksum="73D265E67852C685" modified="1997-11-01" version="1">MKIAITGKGGVGKTFIASTLMRLFEKNGFKVIGVDCDPNPTLALAFGVEEEIVPLSKRHDIIEERTGAKPGTYGNIFKINPKVDDLIDKVGYKIGNITLLVMGTIEEGGEGCVCPASVLLRRLLRHLILKRDEVVILDMEAGIEHFGRKTIDTVDLMLIVIEPTKKSLITAKRMKKLANDLGIKNLGVIVNKVRNEDKELLKDIIKEELGLEVLGFVPYDEEVIKSEFLGKPINLDSKAAKEIEKIFNYIIKLKNTT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58254</accession>
    <name>MCRC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase I operon protein C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mcrC</name>
        <name type="ordered locus">MJ0844</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">MCR is composed of three subunits: alpha, beta, and gamma. The function of proteins C and D is not known.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64405">
        <property type="entry name" value="D64405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870358.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58254"/>
    <dbReference type="STRING" id="243232.MJ_0844"/>
    <dbReference type="DNASU" id="1451732"/>
    <dbReference type="EnsemblBacteria" id="AAB98849">
        <property type="protein sequence ID" value="AAB98849"/>
        <property type="gene ID" value="MJ_0844"/>
    </dbReference>
    <dbReference type="GeneID" id="1451732"/>
    <dbReference type="KEGG" id="mja:MJ_0844"/>
    <dbReference type="eggNOG" id="arCOG03225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099719_0_0_2"/>
    <dbReference type="InParanoid" id="Q58254"/>
    <dbReference type="OMA" id="PVCEITF"/>
    <dbReference type="OrthoDB" id="65557at2157"/>
    <dbReference type="PhylomeDB" id="Q58254"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007687">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003137">
        <property type="entry name" value="McrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03264">
        <property type="entry name" value="met_CoM_red_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147486" description="Methyl-coenzyme M reductase I operon protein C">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <sequence length="200" mass="21668" checksum="B5DA112CA902F24C" modified="1996-11-01" version="1">MPVGRREQIVDCRSVMGLGEGGGLAQRGTFAEALKNDVVVVAMSPGRRHITKPVCEITYGIREAGIQTSVLVLNAGSGIPHDAPRGALGSTFGIKPEEAEQINRHKLCVVHFGNVISHIVYKAGLLLKYVEIPTIIVCQAPVDMEDLAKYGIKTRDVMPLEPKTKGTVVDIVTGVIRGESCPQTKIDEVIRKIKLHLNLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58262</accession>
    <name>MTRF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit F</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrF</name>
        <name type="ordered locus">MJ0852</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrF family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64406">
        <property type="entry name" value="D64406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870366.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58262"/>
    <dbReference type="STRING" id="243232.MJ_0852"/>
    <dbReference type="EnsemblBacteria" id="AAB98857">
        <property type="protein sequence ID" value="AAB98857"/>
        <property type="gene ID" value="MJ_0852"/>
    </dbReference>
    <dbReference type="GeneID" id="1451740"/>
    <dbReference type="KEGG" id="mja:MJ_0852"/>
    <dbReference type="eggNOG" id="arCOG03381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204306_0_0_2"/>
    <dbReference type="OMA" id="LYIYLKM"/>
    <dbReference type="OrthoDB" id="124037at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01099">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011307">
        <property type="entry name" value="MeTrfase_F"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013347">
        <property type="entry name" value="MeTrfase_F_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09472">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006523">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02507">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147548" description="Tetrahydromethanopterin S-methyltransferase subunit F">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="68" mass="7259" checksum="ADA19238B424D169" modified="1997-11-01" version="1">MGVEVSNKPNVSSIQSYVEDLEYKVGLITRNRGLESGTESAGTKGLIIGVVSAIVLMGIPLALYFLMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58296</accession>
    <name>Y886_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative molybdopterin biosynthesis protein MJ0886</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0886</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text>Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64410">
        <property type="entry name" value="F64410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870400.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58296"/>
    <dbReference type="SMR" id="Q58296"/>
    <dbReference type="STRING" id="243232.MJ_0886"/>
    <dbReference type="PRIDE" id="Q58296"/>
    <dbReference type="EnsemblBacteria" id="AAB98890">
        <property type="protein sequence ID" value="AAB98890"/>
        <property type="gene ID" value="MJ_0886"/>
    </dbReference>
    <dbReference type="GeneID" id="1451775"/>
    <dbReference type="KEGG" id="mja:MJ_0886"/>
    <dbReference type="eggNOG" id="arCOG00217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_3_0_2"/>
    <dbReference type="InParanoid" id="Q58296"/>
    <dbReference type="OMA" id="IEAKTHS"/>
    <dbReference type="OrthoDB" id="51611at2157"/>
    <dbReference type="PhylomeDB" id="Q58296"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018315">
        <property type="term" value="P:molybdenum incorporation into molybdenum-molybdopterin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12727">
        <property type="entry name" value="PBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000171005" description="Putative molybdopterin biosynthesis protein MJ0886">
        <location>
            <begin position="1"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="620" mass="68897" checksum="9B94AAA7CA6DF0AB" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q58299</accession>
    <name>Y889_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0889</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0889</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64411">
        <property type="entry name" value="A64411"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58299"/>
    <dbReference type="STRING" id="243232.MJ_0889"/>
    <dbReference type="EnsemblBacteria" id="AAB98905">
        <property type="protein sequence ID" value="AAB98905"/>
        <property type="gene ID" value="MJ_0889"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0889"/>
    <dbReference type="eggNOG" id="arCOG08296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_587451_0_0_2"/>
    <dbReference type="OMA" id="NNRYITN"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107093" description="Uncharacterized protein MJ0889">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <sequence length="469" mass="53586" checksum="F32A0533F531B5C6" modified="1996-11-01" version="1">MVGFMIALSVSSGYLLDNNYSEYSYYILDNDEDFLVFVICDEPNTLKCGFRISEIFSRVFCNAVYNNRYITNLKNLIEVGIYEALENMDKFLKQKGLDYNDLNVSIAGGVYRNGKLMLFSLGNSPVFVVDKDYYLYCPFDLNKNYNLKDWKKFIKFSEVIENPKSVVACSNKLDGKVFKFKNENNKLIAEPFKYRIIQLINSIISNRENIDKIKEELKTDLNLKDNTPLFVASFDEKPINDELVKVGKPKLKVRGCTPILSKKKEETSPHIEPKIIKNMVLAFILILILIFGLFVMLSHNNLNGGNNSDIVNNSSYQVNTSKISIITVNNENKSTNISLNPIQTVKTVSSLNNLDLNNTITKSKNKDSLKILVKFQVGEISKDIKKLPISIMFPEQGYYYISIKSENSNLKLIDVENGEVVYKNTTLIELFEKIDGKEKTYNLIVEYNGSEIPSEKGINISIFEIKIIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58317</accession>
    <name>Y907_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0907</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0907</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64413">
        <property type="entry name" value="C64413"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58317"/>
    <dbReference type="SMR" id="Q58317"/>
    <dbReference type="STRING" id="243232.MJ_0907"/>
    <dbReference type="EnsemblBacteria" id="AAB98909">
        <property type="protein sequence ID" value="AAB98909"/>
        <property type="gene ID" value="MJ_0907"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0907"/>
    <dbReference type="eggNOG" id="arCOG00945">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_3_2_2"/>
    <dbReference type="InParanoid" id="Q58317"/>
    <dbReference type="OMA" id="CYAREFL"/>
    <dbReference type="PhylomeDB" id="Q58317"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="PTHR43273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000134466" description="Uncharacterized protein MJ0907">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="286" mass="32947" checksum="FF488030DC364F94" modified="1996-11-01" version="1">MVDGMKHLILKVTNRCNLNCIYCYANNKNNKDMDFKTAKNAIDYLLNLDNQIKIQFTGGEPLLNFNLIEKIVDYCNDNYSNCNIQYAIQTNATLINEKIAEKIKELDIKVGISIDGLEINDILRPYKNGKPSTLDTLKGMYILKSYNIPFGITTVVTNKNLPYLEEFVKYLIAFGVKSISFDLLKPKKKEHLTLMPNIEEFNKLLNKLGRYPIYIKNLQKRPKDKYCYLNSGDLLFVNEFGDIYLCPTLEGLSCLGNINDKNKIKLPKVKSKGCYAREFLIKTFKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58333</accession>
    <name>Y923_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0923</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0923</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64415">
        <property type="entry name" value="C64415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870437.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58333"/>
    <dbReference type="EnsemblBacteria" id="AAB98936">
        <property type="protein sequence ID" value="AAB98936"/>
        <property type="gene ID" value="MJ_0923"/>
    </dbReference>
    <dbReference type="GeneID" id="1451812"/>
    <dbReference type="KEGG" id="mja:MJ_0923"/>
    <dbReference type="eggNOG" id="arCOG06562">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_938808_0_0_2"/>
    <dbReference type="OMA" id="GREVFYY"/>
    <dbReference type="OrthoDB" id="57604at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107108" description="Uncharacterized protein MJ0923">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="297" mass="34083" checksum="8ECEF68FFBD70D24" modified="1996-11-01" version="1">MEIFKLLRKDRKMLFLMFIGTVSFLFIFIPFLKFQMIGSSHKINAYPSLSAVCGLLLGPIYGFFAVMLVTLIYFFLNPKAFYFGIYSLIPPTLAVISAGALSEGKWKYSAIILIVGLLLFYLTDVGRVAFYYPYLSTLALLLILIFREKISKLLFSKDWKKMIVGATILSFSSVMTDHLYGSILGIVYLHLPAEDYISVIPLFIKERLIMTVIGAFFVIFAIEISKCFLKNATKLKEKLLKSYIDKEIKINCKNMLNVDEELLKKYNVKIPSEEEQKEILKTLVEVVVFNNDKDENR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58340</accession>
    <name>CBIC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt-precorrin-8 methylmutase</fullName>
            <ecNumber>5.4.99.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cobalt-precorrin isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cbiC</name>
        <name type="synonym">cobH</name>
        <name type="ordered locus">MJ0930</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of cobalt-precorrin-8 to cobyrinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Co-precorrin-8X = cob(II)yrinate</text>
            <dbReference type="Rhea" id="RHEA:16209"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:70792"/>
            <dbReference type="EC" id="5.4.99.60"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 9/10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.60"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64416">
        <property type="entry name" value="B64416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870444.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58340"/>
    <dbReference type="SMR" id="Q58340"/>
    <dbReference type="STRING" id="243232.MJ_0930"/>
    <dbReference type="EnsemblBacteria" id="AAB98932">
        <property type="protein sequence ID" value="AAB98932"/>
        <property type="gene ID" value="MJ_0930"/>
    </dbReference>
    <dbReference type="GeneID" id="1451819"/>
    <dbReference type="KEGG" id="mja:MJ_0930"/>
    <dbReference type="eggNOG" id="arCOG02247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084703_1_1_2"/>
    <dbReference type="InParanoid" id="Q58340"/>
    <dbReference type="OMA" id="GAPIFCD"/>
    <dbReference type="OrthoDB" id="71138at2157"/>
    <dbReference type="PhylomeDB" id="Q58340"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00230"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043778">
        <property type="term" value="F:cobalt-precorrin-8 methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="SSF63965"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135928" description="Cobalt-precorrin-8 methylmutase">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="210" mass="22974" checksum="2B5773E46E3105E6" modified="1996-11-01" version="1">MGAISKDGLNIANKSREIVRNKIKEVLGDRINEFNEKEMGIIERVVHATADPEYAKLLVFKNNPIEEGIKAIKEEKPIIVDVNMIKAGIRYNKVHCFINHPDVYEVAKKEGITRAVASMRLAKDLIDDGIVVIGNSPTALFEVIRLVKEENIKPKLIVGVPVGFVQASESKEALREVNVPSISTIGPKGGTPVAVAIINGIIAYAKNEKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58387</accession>
    <name>RECJ_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Single-stranded-DNA-specific exonuclease RecJ</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">recJ</name>
        <name type="ordered locus">MJ0977</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="607" last="612">
            <title>A thermostable single-strand DNase from Methanococcus jannaschii related to the RecJ recombination and repair exonuclease from Escherichia coli.</title>
            <authorList>
                <person name="Rajman L.A."/>
                <person name="Lovett S.T."/>
            </authorList>
            <dbReference type="PubMed" id="10633092"/>
            <dbReference type="DOI" id="10.1128/JB.182.3.607-612.2000"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Single-stranded-DNA-specific exonuclease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RecJ family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64422">
        <property type="entry name" value="A64422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870491.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58387"/>
    <dbReference type="SMR" id="Q58387"/>
    <dbReference type="STRING" id="243232.MJ_0977"/>
    <dbReference type="EnsemblBacteria" id="AAB98980">
        <property type="protein sequence ID" value="AAB98980"/>
        <property type="gene ID" value="MJ_0977"/>
    </dbReference>
    <dbReference type="GeneID" id="1451875"/>
    <dbReference type="KEGG" id="mja:MJ_0977"/>
    <dbReference type="eggNOG" id="arCOG00427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042622_0_0_2"/>
    <dbReference type="InParanoid" id="Q58387"/>
    <dbReference type="OMA" id="ACGAYIP"/>
    <dbReference type="OrthoDB" id="45290at2157"/>
    <dbReference type="PhylomeDB" id="Q58387"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000097231" description="Single-stranded-DNA-specific exonuclease RecJ">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="10633092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="469" mass="53847" checksum="037110C6D53B7554" modified="1996-11-01" version="1">MENWVELKKGAKVLQNNKNNKILIVTHIDTDGLTSRAILQKLAERLNLDADFMFLKQITIETINDIPFKDYDLIIFADLGSGQLKMIKEKLDELNLSDKRDKIIILDHHQPEEIKIPKTIIHINPLTIAKSGAEICGAGVSYLFAKAINNDWIDLAKYAVLGAVGDIQNIEGKLIGLNRKILCDAIMNGDVKVKTDLQMYGRQTRPLFVSMRYWADVRTDLLNNDSKIIKYIQSINKKYDIEINPTMRLAEIPFEHKRIIGNELLIKCLNYVPNHWTPYIPKVIFGEVYEFRGEEFGSPLRDLEEFSTCINACSRYGDYETALNVLMGDRDKYYRKMLSNLRKHRNNLREALEHVKNDVEIIQKDRFQYFETDKIMSNIVGIVAGMSYSIEEVDWMKPIFAITEDENGYKVSARCPKLLCFAEDVNLAKAIKYASEKVNGSGGGHKFACGAYIPDNKREFIKYLEIALR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58396</accession>
    <name>Y989_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0989</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0989</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.kandleri MK0008.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64423">
        <property type="entry name" value="E64423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870503.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58396"/>
    <dbReference type="SMR" id="Q58396"/>
    <dbReference type="STRING" id="243232.MJ_0989"/>
    <dbReference type="EnsemblBacteria" id="AAB98991">
        <property type="protein sequence ID" value="AAB98991"/>
        <property type="gene ID" value="MJ_0989"/>
    </dbReference>
    <dbReference type="GeneID" id="8805478"/>
    <dbReference type="KEGG" id="mja:MJ_0989"/>
    <dbReference type="eggNOG" id="arCOG02068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151801_2_0_2"/>
    <dbReference type="InParanoid" id="Q58396"/>
    <dbReference type="OMA" id="VKACGPC"/>
    <dbReference type="OrthoDB" id="91267at2157"/>
    <dbReference type="PhylomeDB" id="Q58396"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107132" description="Uncharacterized protein MJ0989">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="114" mass="12577" checksum="2FF7A56356C0E3FB" modified="1996-11-01" version="1">MKFTVIITEAPYGKERAYSALRFALTALLEGIEVNIFLLENGVYVAKKEQNPSEVPNYLELLKNAIELGAVVKVCGPCCKARGLKEEDLIEGAKLATMHDLIAFVKESDNVVTF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58411</accession>
    <name>Y1005_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1005</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1005</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64425">
        <property type="entry name" value="D64425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870518.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58411"/>
    <dbReference type="SMR" id="Q58411"/>
    <dbReference type="STRING" id="243232.MJ_1005"/>
    <dbReference type="EnsemblBacteria" id="AAB99013">
        <property type="protein sequence ID" value="AAB99013"/>
        <property type="gene ID" value="MJ_1005"/>
    </dbReference>
    <dbReference type="GeneID" id="1451902"/>
    <dbReference type="KEGG" id="mja:MJ_1005"/>
    <dbReference type="eggNOG" id="arCOG05062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158394_0_0_2"/>
    <dbReference type="OMA" id="SGPFGGY"/>
    <dbReference type="OrthoDB" id="93269at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107140" description="Uncharacterized protein MJ1005">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <sequence length="131" mass="15579" checksum="3417CF17A408481E" modified="1996-11-01" version="1">MRIEDEIKNIIEKKDYDFWEFLKKAYENNIKLDIGHFILLNILIGVNDLYHKLSEKFGEEEARKILERNKIFAKNSDFISGEFLKDYIDRKSRVAVHNRIKDLKTLGFKIESKSGPFGGYKIVGYPEWFKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58421</accession>
    <name>PSTS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphate-binding protein PstS</fullName>
            <shortName>PBP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">pstS</name>
        <name type="ordered locus">MJ1015</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PstS family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64426">
        <property type="entry name" value="F64426"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58421"/>
    <dbReference type="SMR" id="Q58421"/>
    <dbReference type="STRING" id="243232.MJ_1015"/>
    <dbReference type="EnsemblBacteria" id="AAB99019">
        <property type="protein sequence ID" value="AAB99019"/>
        <property type="gene ID" value="MJ_1015"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1015"/>
    <dbReference type="eggNOG" id="arCOG00213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034528_1_2_2"/>
    <dbReference type="InParanoid" id="Q58421"/>
    <dbReference type="OMA" id="FPYPVYA"/>
    <dbReference type="PhylomeDB" id="Q58421"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000062778" description="Phosphate-binding protein PstS">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P9WGT7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="389" mass="42414" checksum="48B54C6A953ADAC9" modified="1996-11-01" version="1">MNDTTQPTKGDAVKKILALILGLCLIVPVISIAGCVGGGNSQPSNNEKPSTIIIRTTGATFPKYQIQKWIEDYQKTHPNVKIEYEGGGSGYGQEAFAKGLTDIGRTDPPVKESMWKKFLSTGDQPLQFPEIVGAVVVTYNIPEIGDKTLKLSRDVLADIFLGKIEYWDDERIKKINPEIADKLPHEKIIVVHRSDASGTTAIFTTYLSLISKEWAEKVGAGKTVNWPTDNIGRGVAGKGNPGVVAIVKSTPYTVAYTELSYAIEQKLPVAALENKNGKFVKPTDETIKAAVSAVKASIPNPTEGYKEDLKQMLDAPGDNAYPIVAFTHLLVWENKNGKHYSPEKAKAIKDFLTWVLTEGQKPEHLAPGYVGLPEDVAKIGLNAVNMIKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58426</accession>
    <name>Y1020_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1020</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1020</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64427">
        <property type="entry name" value="C64427"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58426"/>
    <dbReference type="STRING" id="243232.MJ_1020"/>
    <dbReference type="DNASU" id="1451917"/>
    <dbReference type="EnsemblBacteria" id="AAB99024">
        <property type="protein sequence ID" value="AAB99024"/>
        <property type="gene ID" value="MJ_1020"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1020"/>
    <dbReference type="eggNOG" id="arCOG04230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068640_0_0_2"/>
    <dbReference type="InParanoid" id="Q58426"/>
    <dbReference type="OMA" id="LHAILCH"/>
    <dbReference type="PhylomeDB" id="Q58426"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039967">
        <property type="entry name" value="MJ1020-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40517">
        <property type="entry name" value="PTHR40517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107144" description="Uncharacterized protein MJ1020">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="51"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <sequence length="259" mass="29218" checksum="1CACA2E63B82592B" modified="1996-11-01" version="1">MGRDMDFEELNSLQGIPKMIYDELIKNKKVNTFLKMSNIMAVGRLGYNDHGKTHAKIVANNAIKMLKILYKKGIEPSFVKDCKGSFEDSLVITLLGAYLHDIGNSVHRDIHHLHSAYLALDIVENILKKYYKEEKAYQMTTEVLHAIYSHSEGIMGLTIEAGVIAVADGTDMTKGRSRIPICKKCYDIHSVSAASIERVEIKEGDEKPIQIEVILSNEAGIFQIQEVLGEKIKWSGIKNYVSVYARVEKEKPVFEEITI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58457</accession>
    <name>Y1057_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized glycosyltransferase MJ1057</fullName>
            <ecNumber>2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1057</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64431">
        <property type="entry name" value="H64431"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1057"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99061">
        <property type="protein sequence ID" value="AAB99061"/>
        <property type="gene ID" value="MJ_1057"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1057"/>
    <dbReference type="eggNOG" id="arCOG01381">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025996_0_4_2"/>
    <dbReference type="InParanoid" id="Q58457"/>
    <dbReference type="OMA" id="ICLCYNH"/>
    <dbReference type="PhylomeDB" id="Q58457"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000059252" description="Uncharacterized glycosyltransferase MJ1057">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="290" mass="35103" checksum="3F6A1B221C420D74" modified="2000-05-30" version="2">MVGEMDKPLVSVVMATYNEPEKYLKESIESIXNQTXKDFXFIIVLDNPNNKKAEEIIKEYQQKDKRIIFIKNERNLGRGASRNKAVNIARGKYIAILDADDIALPKRLEKQFKYMENNRDIDLLFSWVYFIDENGNILKEFKPEKYKFKEIKKYFFKEHLTVHPSMMVKSKILKKLKYDEKLIRSQDYDFWIRCIANDYKFDIIEEFLLKYRIPNRDNYLSRIKKQKLYSYYTLKTHWKNKKHFCNNVYFWKVFFYSLVVYLFIVLTPTFILKILIDIKDKKTEISTKGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58459</accession>
    <name>Y1059_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized glycosyltransferase MJ1059</fullName>
            <ecNumber>2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1059</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64432">
        <property type="entry name" value="B64432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870572.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58459"/>
    <dbReference type="SMR" id="Q58459"/>
    <dbReference type="STRING" id="243232.MJ_1059"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="DNASU" id="1451956"/>
    <dbReference type="EnsemblBacteria" id="AAB99063">
        <property type="protein sequence ID" value="AAB99063"/>
        <property type="gene ID" value="MJ_1059"/>
    </dbReference>
    <dbReference type="GeneID" id="1451956"/>
    <dbReference type="KEGG" id="mja:MJ_1059"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_0_0_2"/>
    <dbReference type="InParanoid" id="Q58459"/>
    <dbReference type="OMA" id="YESKYIS"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="PhylomeDB" id="Q58459"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13477">
        <property type="entry name" value="Glyco_trans_4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000080321" description="Uncharacterized glycosyltransferase MJ1059">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="406" mass="48270" checksum="7CB71CDDB02C7F9D" modified="1996-11-01" version="1">MSNKKKQLTVMGTVWDFWSVLKMFDKLYESKYISFYEPWLKGEIDKEKIILFNEKSKNPLLWPFKILKRTYKILKIIREFKPDLVITHHDDANVSIIPVILLNKIFKISNNTKFILWVRNNPIESYKEGLYSKIIILAYKYFYKYADIIIVQTQENKKIIESHFKSLKNKTKIVPNVYEIDKLQQLSNEPLEKQYRNIFKDSFVFINIGRLTEQKGQWFLIRSFKRVTEKYPNAKLIILGDGELKNKLQELINKLNLQNNVYLLGMQKNPFKFLKHSNCFVFSSLWEGLPNTVIEALSLNLPVISTDCKTGPREILCPELNISDKIDYPYYGKYGILTKPFSREFIWQDLNEKPLIEEEKMLADLMIKMIEDEDLRKRYSNGLERAKDFDIEKIIKEWKLLIEGTI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58465</accession>
    <name>Y1065_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1065</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1065</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To B.subtilis SpsE.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64432">
        <property type="entry name" value="H64432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870578.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58465"/>
    <dbReference type="SMR" id="Q58465"/>
    <dbReference type="STRING" id="243232.MJ_1065"/>
    <dbReference type="EnsemblBacteria" id="AAB99068">
        <property type="protein sequence ID" value="AAB99068"/>
        <property type="gene ID" value="MJ_1065"/>
    </dbReference>
    <dbReference type="GeneID" id="1451962"/>
    <dbReference type="KEGG" id="mja:MJ_1065"/>
    <dbReference type="eggNOG" id="arCOG01050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040465_0_0_2"/>
    <dbReference type="InParanoid" id="Q58465"/>
    <dbReference type="OMA" id="YRHRVEF"/>
    <dbReference type="OrthoDB" id="53088at2157"/>
    <dbReference type="PhylomeDB" id="Q58465"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047444">
        <property type="term" value="F:N-acylneuraminate-9-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070085">
        <property type="term" value="P:glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006190">
        <property type="entry name" value="AFP_Neu5c_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036732">
        <property type="entry name" value="AFP_Neu5c_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013132">
        <property type="entry name" value="Neu5Ac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013974">
        <property type="entry name" value="SAF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03102">
        <property type="entry name" value="NeuB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08666">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00858">
        <property type="entry name" value="SAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51269">
        <property type="entry name" value="SSF51269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50844">
        <property type="entry name" value="AFP_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107156" description="Uncharacterized protein MJ1065">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="AFP-like" evidence="1">
        <location>
            <begin position="279"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="337" mass="37977" checksum="790CCAFF48C1111B" modified="1996-11-01" version="1">MEKIKIGDRYVGKGEPTFIIAEGGLNHNGDIDIGKELVKEAKKCGADAIKFQSYHTEDFISKKSEYYELFKSLELSEEEFYELKEYAEKIGIMFISTPLDLKYVDILNKMNVPAFKIASGDLTFYPLLEKVAKTGKPVILSTGMSDIGEIWEAVKVLENNGCRDIILLHCISSYPTPYEDVNLNAIKTLKSIFNIPVGYSDHTLGILAPVVSVALGADVIEKHFTLDKNMEGPDHALSADPEEFKEMVNNIRLVEKMLGSGEKIPMPSERDVIVEARRSIVAKRNIKKGEYLSVDNISFKRPGRGIETKYLSIILNRKIKNDKEEDDIIYWDDLLGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58469</accession>
    <name>Y1069_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized glycosyltransferase MJ1069</fullName>
            <ecNumber>2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1069</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64433">
        <property type="entry name" value="D64433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870581.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58469"/>
    <dbReference type="SMR" id="Q58469"/>
    <dbReference type="STRING" id="243232.MJ_1069"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="DNASU" id="1451965"/>
    <dbReference type="EnsemblBacteria" id="AAB99071">
        <property type="protein sequence ID" value="AAB99071"/>
        <property type="gene ID" value="MJ_1069"/>
    </dbReference>
    <dbReference type="GeneID" id="1451965"/>
    <dbReference type="KEGG" id="mja:MJ_1069"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_5_2"/>
    <dbReference type="InParanoid" id="Q58469"/>
    <dbReference type="OMA" id="MTRTRFF"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="PhylomeDB" id="Q58469"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000080322" description="Uncharacterized glycosyltransferase MJ1069">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="392" mass="45820" checksum="264254846109A4E8" modified="1996-11-01" version="1">MRKIKLIIFPGYYIPHIGGLETHVDEFTKHLSEDENYDIYIFAPNIPKYKEFEIRHNNVKVYRYPAFEIIPNYPVPNIFNIKFWRMFFNLYKIDFDIVMTRTRFFSNTLLGFIFAKLRFKKKKLIHVEHGSAFVKLESEFKNKLSYFYDKTIGKLIFKKADYVVAISKAVKNFILENFVNDKDIPIIYRGLEIEKIESIGEDKKIKEKFKNKIKLCFVGRLYKWKGVENIIKAYVDLPKDLKEKIILIVVGYGEDLERLKKLAGNYLNNGIYFTGKVDFEKAIAIVKASDIYIHSSYKGGGLSSSLLQAMCCGKAIVASPYEGADEVVIDGYNGILLKDNSPEEIKRGIIKLIENNNLRKIYGENAKNFIKENFNWKKSVKEYKKIFERLVN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58473</accession>
    <name>RIO2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>RIO-type serine/threonine-protein kinase Rio2</fullName>
            <ecNumber>2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rio2</name>
        <name type="ordered locus">MJ1073</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64433">
        <property type="entry name" value="H64433"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58473"/>
    <dbReference type="SMR" id="Q58473"/>
    <dbReference type="STRING" id="243232.MJ_1073"/>
    <dbReference type="EnsemblBacteria" id="AAB99073">
        <property type="protein sequence ID" value="AAB99073"/>
        <property type="gene ID" value="MJ_1073"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1073"/>
    <dbReference type="eggNOG" id="arCOG01181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_1_0_2"/>
    <dbReference type="InParanoid" id="Q58473"/>
    <dbReference type="OMA" id="GYTNFRE"/>
    <dbReference type="PhylomeDB" id="Q58473"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000213535" description="RIO-type serine/threonine-protein kinase Rio2">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="2">
        <location>
            <begin position="65"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="72"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="158"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="206"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="270" mass="31881" checksum="837BCE317A8A5CDF" modified="1996-11-01" version="1">MRHHEWVPLDEIVRKAKMPEKDVLYRLKRLNKFGFVVRSTYGYAVSMGGYDALAINAFVKKGILKAIGNKLGVGKEGDVYTVLLSDGREAVLKFHKHGRTCFTRGKRYRGYLADKHHISWLYVSRLTAEREFEILNELFPIVKVPEPIEWNRHAIIMGKVVGEELKRLDLSEFMSKEEIKDLFWKIIEEVKKAYEIGYIHGDLSEFNILLDENGDFVIIDWPQAVPKYHPDAEFYLKRDIWNVIRYFKKYKIDKEDEKIDVDKIFEYITK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58479</accession>
    <name>Y1079_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1079</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1079</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64434">
        <property type="entry name" value="F64434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870591.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58479"/>
    <dbReference type="STRING" id="243232.MJ_1079"/>
    <dbReference type="EnsemblBacteria" id="AAB99076">
        <property type="protein sequence ID" value="AAB99076"/>
        <property type="gene ID" value="MJ_1079"/>
    </dbReference>
    <dbReference type="GeneID" id="1451975"/>
    <dbReference type="KEGG" id="mja:MJ_1079"/>
    <dbReference type="eggNOG" id="arCOG04469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043916_1_0_2"/>
    <dbReference type="InParanoid" id="Q58479"/>
    <dbReference type="OMA" id="FVGCAML"/>
    <dbReference type="OrthoDB" id="112841at2157"/>
    <dbReference type="PhylomeDB" id="Q58479"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107160" description="Uncharacterized protein MJ1079">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="397" mass="44268" checksum="D6AC13687ED1FB54" modified="1996-11-01" version="1">MLNLLYLILGIICGTITGLFPGIHPNNIVALSFLILPYFGLDNYIPFLIGLVITHYFINFIPSAFLGVPDDETAVSALPMHKLTLNGNGYEAIVLAGFGSYLGVVFSILISLFLMSILHFDVRAFYCSIKIFIPFILIAFILYQIFTAKSVWEVLVIFLSGIFGIAVLYCSEAFNITLTAIFTGMFGIPLLINNLKTYKIKSQMMAFPDFELKFLKSSFFASVAGFFRIFLPGISGAQLNYILSKILNERDLKNFIVSQGSIILSNEVFSLLAVIFIGVGRSGVARAIQLLNANININTAIFSILISSTIAIIILLNLSKYILLFIRKVNFKFLSLFFIIFCSLVVIIGSYNTYLIYHIIVYLTAIYIGLLAVKSNTNLSNMMNVLIFPTILYFLRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58528</accession>
    <name>Y1128_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1128</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1128</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64440">
        <property type="entry name" value="G64440"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58528"/>
    <dbReference type="SMR" id="Q58528"/>
    <dbReference type="STRING" id="243232.MJ_1128"/>
    <dbReference type="DNASU" id="1452024"/>
    <dbReference type="EnsemblBacteria" id="AAB99130">
        <property type="protein sequence ID" value="AAB99130"/>
        <property type="gene ID" value="MJ_1128"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1128"/>
    <dbReference type="eggNOG" id="arCOG07790">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_910928_0_0_2"/>
    <dbReference type="OMA" id="CQAGCLK"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047464">
        <property type="term" value="F:heparosan-N-sulfate-glucuronate 5-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010598">
        <property type="entry name" value="C5-epim_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039721">
        <property type="entry name" value="C5-epimerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13174">
        <property type="entry name" value="PTHR13174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06662">
        <property type="entry name" value="C5-epim_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014008" description="Uncharacterized protein MJ1128">
        <location>
            <begin position="29"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="308" mass="36443" checksum="48FCB2EB001B5091" modified="1996-11-01" version="1" precursor="true">MILMKKFEIILFLFIAVLIFVFGYFVGASQPLYSENPVIQYFKNPKPFTVENVNMPVTYYGTICGKYIGYQITPHNVNEEARKCFYKYFKLKDKNPKEAERYLKRGLFLTEYLISQADKETAEVDEKNITFIVWRYNFEFPLYNLSKGWRGALCQAGCLKTLYLAYEATGDERYLNYANLAINAFKVPVEKGGLLKIRIYKNKSYYWFPEYASENPPYVLNGFITATLWIGDFGNKTGNADALYLYKEGLKSIKTFLPMYDAGDWSYYDALGHRCNKHYEHLHRLQMLWLYNKTGDEIYLKYYKKWRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58608</accession>
    <name>Y1211_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1211</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1211</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64451">
        <property type="entry name" value="B64451"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58608"/>
    <dbReference type="SMR" id="Q58608"/>
    <dbReference type="STRING" id="243232.MJ_1211"/>
    <dbReference type="EnsemblBacteria" id="AAB99222">
        <property type="protein sequence ID" value="AAB99222"/>
        <property type="gene ID" value="MJ_1211"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1211"/>
    <dbReference type="eggNOG" id="arCOG01182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091298_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58608"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107215" description="Uncharacterized protein MJ1211">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <sequence length="116" mass="13422" checksum="0EA661BCFC5EAA7C" modified="1996-11-01" version="1">MEFIDGEELKSAVDKLDKDRLLKVVEDILKITLKLDILGIEHKEIQGGRHFLITNKKTYIIDFDKAKEKKTTKNFTGAIALLFGEGRIAKTIRENLNIGIDEIKFIREFAKKYKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58615</accession>
    <name>T1S2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type I restriction enzyme MjaVIII specificity subunit</fullName>
            <shortName>S protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Type I specificity subunit S.MjaVIII</fullName>
            <shortName evidence="2">S.MjaVIII</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1218</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">The specificity (S) subunit of a type I restriction enzyme; this subunit dictates DNA sequence specificity. The M and S subunits together form a methyltransferase (MTase) that methylates A-2 on the top and A-3 on the bottom strand of the sequence 5'-GAYN(5)GTAA-3'. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg(2+) as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64452">
        <property type="entry name" value="A64452"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58615"/>
    <dbReference type="SMR" id="Q58615"/>
    <dbReference type="STRING" id="243232.MJ_1218"/>
    <dbReference type="REBASE" id="3904">
        <property type="entry name" value="S.MjaVIII"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99219">
        <property type="protein sequence ID" value="AAB99219"/>
        <property type="gene ID" value="MJ_1218"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1218"/>
    <dbReference type="eggNOG" id="arCOG02626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021095_0_1_2"/>
    <dbReference type="InParanoid" id="Q58615"/>
    <dbReference type="OMA" id="PEDWEVK"/>
    <dbReference type="PhylomeDB" id="Q58615"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000107220" description="Type I restriction enzyme MjaVIII specificity subunit">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P05719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="425" mass="49410" checksum="5681C338F957C49F" modified="1997-11-01" version="1">MVIIMQFYKEENFKEMHGLRVPEDWEVVRIGDFIKYIKGKKPAVMVDEELEGYYPYLSTEYLRDGIASKFVKITNKEIIVNENDILLLWDGSNAGEIFLGKKGILSSTMVKLEQKNKIMDDLYLFYSLKLKESFLKSQTKGTGIPHVDKKIFENIKIPLPPLEEQKQIAKILSDFDNLIGTINKQIEVLNKAKKGMMKKLFTKGVFEHKSFKKSEIGEIPEDWEVVKLKEVVDIQSGKYFKYSEFCENGVKCLKIDNVGFGKIFWETVSFLPEDYLNKYPQLVLKSGDIVLALNRPIIGGKIKIGILKDIDEPAILYQRVGRFIFKSEKIDKQFLFYLLMSEYFKKELSKLLIGTDQPYIRTPVLLNIKIPLPHLEEQKAMAERLKSIDNLIEIKRKEKEQIEKAKKKIMNLLLTGKIRVKNLNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>Q58625</accession>
    <name>IF5A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">eif5a</name>
        <name type="ordered locus">MJ1228</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Proc. Natl. Acad. Sci. U.S.A." volume="95" first="224" last="228">
            <title>Universally conserved translation initiation factors.</title>
            <authorList>
                <person name="Kyrpides N.C."/>
                <person name="Woese C.R."/>
            </authorList>
            <dbReference type="PubMed" id="9419357"/>
            <dbReference type="DOI" id="10.1073/pnas.95.1.224"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1998" name="Proc. Natl. Acad. Sci. U.S.A." volume="95" first="10419" last="10424">
            <title>Crystal structures of eukaryotic translation initiation factor 5A from Methanococcus jannaschii at 1.8-A resolution.</title>
            <authorList>
                <person name="Kim K.K."/>
                <person name="Hung L.W."/>
                <person name="Yokota H."/>
                <person name="Kim R."/>
                <person name="Kim S.H."/>
            </authorList>
            <dbReference type="PubMed" id="9724718"/>
            <dbReference type="DOI" id="10.1073/pnas.95.18.10419"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)</scope>
        <scope>HYPUSINE AT LYS-37</scope>
    </reference>
    <comment type="function">
        <text>Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-5A family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870740.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1EIF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="2EIF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=1-132"/>
    </dbReference>
    <dbReference type="PDBsum" id="1EIF"/>
    <dbReference type="PDBsum" id="2EIF"/>
    <dbReference type="AlphaFoldDB" id="Q58625"/>
    <dbReference type="SMR" id="Q58625"/>
    <dbReference type="STRING" id="243232.MJ_1228"/>
    <dbReference type="EnsemblBacteria" id="AAB99231">
        <property type="protein sequence ID" value="AAB99231"/>
        <property type="gene ID" value="MJ_1228"/>
    </dbReference>
    <dbReference type="GeneID" id="1452124"/>
    <dbReference type="KEGG" id="mja:MJ_1228"/>
    <dbReference type="eggNOG" id="arCOG04277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102600_3_0_2"/>
    <dbReference type="InParanoid" id="Q58625"/>
    <dbReference type="OMA" id="QIMDMET"/>
    <dbReference type="OrthoDB" id="98221at2157"/>
    <dbReference type="PhylomeDB" id="Q58625"/>
    <dbReference type="EvolutionaryTrace" id="Q58625"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019769">
        <property type="entry name" value="Trans_elong_IF5A_hypusine_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="Transl_elong_IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013185">
        <property type="entry name" value="Transl_elong_KOW-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="PTHR11673"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08207">
        <property type="entry name" value="EFP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00302">
        <property type="entry name" value="IF5A_HYPUSINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0385">Hypusine</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142493" description="Translation initiation factor 5A">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="1">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="16"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="42"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="55"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="64"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="85"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="91"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="113"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="122"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="9724718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2EIF"/>
        </source>
    </evidence>
    <sequence length="132" mass="14180" checksum="5B0F48905B0DCF69" modified="1998-12-15" version="2">MPGTKQVNVGSLKVGQYVMIDGVPCEIVDISVSKPGKHGGAKARVVGIGIFEKVKKEFVAPTSSKVEVPIIDRRKGQVLAIMGDMVQIMDLQTYETLELPIPEGIEGLEPGGEVEYIEAVGQYKITRVIGGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58631</accession>
    <name>CAS6B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endoribonuclease Cas6 2</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas6b</name>
        <name type="ordered locus">MJ1234</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein processes pre-crRNA into individual crRNA units.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64454">
        <property type="entry name" value="A64454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870746.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58631"/>
    <dbReference type="SMR" id="Q58631"/>
    <dbReference type="STRING" id="243232.MJ_1234"/>
    <dbReference type="DNASU" id="1452130"/>
    <dbReference type="EnsemblBacteria" id="AAB99238">
        <property type="protein sequence ID" value="AAB99238"/>
        <property type="gene ID" value="MJ_1234"/>
    </dbReference>
    <dbReference type="GeneID" id="1452130"/>
    <dbReference type="KEGG" id="mja:MJ_1234"/>
    <dbReference type="eggNOG" id="arCOG04342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089858_1_0_2"/>
    <dbReference type="InParanoid" id="Q58631"/>
    <dbReference type="OMA" id="NIGEMPF"/>
    <dbReference type="OrthoDB" id="59332at2157"/>
    <dbReference type="PhylomeDB" id="Q58631"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="PTHR36984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000107230" description="CRISPR-associated endoribonuclease Cas6 2">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8U1S4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="241" mass="28840" checksum="23AD8F19448CBFC6" modified="1996-11-01" version="1">MRLKLSLTPKQDFSFDKINKHTIQGFIYSLLKDTEFGEMHNQPRFKFWCFSDIFPPNDFVKGEDKYLLISSPREEFINVLYERLDNLEEVNLNNFKFEVSELKKFDLKVKNKFITGSPIVLYKDKDRGEYIKFYDDDFDLMFFVQRLQDNAVKKYKAFYNEEPVLNGFIFDRISPRVRNGRVDVYVRIAKKGREFLVVGTTWKLLEKIKIRKEERKFYKFIMDCGLGEKNSLGFGFINPIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58661</accession>
    <name>NDK_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="1">NDK</shortName>
            <shortName evidence="1">NDP kinase</shortName>
            <ecNumber evidence="1">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ndk</name>
        <name type="ordered locus">MJ1265</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Acta Crystallogr. D" volume="56" first="1485" last="1487">
            <title>Nucleoside diphosphate kinase from the hyperthermophilic archaeon Methanococcus jannaschii: overexpression, crystallization and preliminary X-ray crystallographic analysis.</title>
            <authorList>
                <person name="Min K."/>
                <person name="Song H.K."/>
                <person name="Chang C."/>
                <person name="Lee J.Y."/>
                <person name="Eom S.H."/>
                <person name="Kim K.K."/>
                <person name="Yu Y.G."/>
                <person name="Suh S.W."/>
            </authorList>
            <dbReference type="PubMed" id="11053861"/>
            <dbReference type="DOI" id="10.1107/S0907444900011240"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text>Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="1"/>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="EnsemblBacteria" id="AAB99271">
        <property type="protein sequence ID" value="AAB99271"/>
        <property type="gene ID" value="MJ_1265"/>
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    <dbReference type="GeneID" id="1452163"/>
    <dbReference type="KEGG" id="mja:MJ_1265"/>
    <dbReference type="eggNOG" id="arCOG04313">
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    <dbReference type="HOGENOM" id="CLU_060216_6_3_2"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:UTP biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="SSF54919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDP_KINASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137091" description="Nucleoside diphosphate kinase">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="140" mass="16224" checksum="B22C60A248BF21F7" modified="1997-11-01" version="1">MKERTFVALKPDAVKRKLIGKIIERFENKGFEIVAMKMIKLDREMAEKYYEEHKGKEFYERLINFMTSGRMIVMVVEGENAISVVRKMIGKTNPAEAEPGTIRGDFALTTPDNIIHASDSKESAEREIKLFFKEDEIFDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58682</accession>
    <name>Y1286_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1286</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1286</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii FlaJ.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64460">
        <property type="entry name" value="E64460"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58682"/>
    <dbReference type="STRING" id="243232.MJ_1286"/>
    <dbReference type="EnsemblBacteria" id="AAB99288">
        <property type="protein sequence ID" value="AAB99288"/>
        <property type="gene ID" value="MJ_1286"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1286"/>
    <dbReference type="eggNOG" id="arCOG01808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_484538_0_0_2"/>
    <dbReference type="InParanoid" id="Q58682"/>
    <dbReference type="OMA" id="IFVFYPE"/>
    <dbReference type="PhylomeDB" id="Q58682"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107254" description="Uncharacterized protein MJ1286">
        <location>
            <begin position="1"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="334"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="504"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="547"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="571"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="595" mass="68277" checksum="9008BF01EECAC3DC" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58690</accession>
    <name>TTDA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative L(+)-tartrate dehydratase subunit alpha</fullName>
            <shortName evidence="2">L-TTD alpha</shortName>
            <ecNumber evidence="2">4.2.1.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1294</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R,3R)-tartrate = H2O + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:15413"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:30924"/>
            <dbReference type="EC" id="4.2.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I fumarase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.32" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64461">
        <property type="entry name" value="E64461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870811.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58690"/>
    <dbReference type="SMR" id="Q58690"/>
    <dbReference type="STRING" id="243232.MJ_1294"/>
    <dbReference type="EnsemblBacteria" id="AAB99301">
        <property type="protein sequence ID" value="AAB99301"/>
        <property type="gene ID" value="MJ_1294"/>
    </dbReference>
    <dbReference type="GeneID" id="1452196"/>
    <dbReference type="KEGG" id="mja:MJ_1294"/>
    <dbReference type="eggNOG" id="arCOG04407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041245_0_0_2"/>
    <dbReference type="InParanoid" id="Q58690"/>
    <dbReference type="OMA" id="IETYPTH"/>
    <dbReference type="OrthoDB" id="59419at2157"/>
    <dbReference type="PhylomeDB" id="Q58690"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008730">
        <property type="term" value="F:L(+)-tartrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008152">
        <property type="term" value="P:metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000195661" description="Putative L(+)-tartrate dehydratase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="E9AE57"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P05847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="285" mass="31088" checksum="49BA64703BBC7ABC" modified="1996-11-01" version="1">MKISDVVVELFREAAIYLPEDVKNALEEAYKKESSEISKNTLKAIIENNKIAEETQVPLCQDTGVPIVFLKIGKNINSSEIMKIIEEIKEGVKKATEEVPLRPNVVHPLTRENFKTNVGLNSPFINIEFDESLDREIEIIAFPKGAGSENMSALKMLKPSDGIEGIKNFVLETIANAGGKPCPPIVVGIGIGGTADVALKLAKKALLRKIGERHRDKEIANLEKELLEKINSLGIGAMGLGGDITALDVFIEIAGCHTASLPVGICIQCWADRRAIKRIKLDAKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q58725</accession>
    <name>MAP2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine aminopeptidase</fullName>
            <shortName evidence="1">MAP</shortName>
            <shortName evidence="1">MetAP</shortName>
            <ecNumber evidence="1">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">map</name>
        <name type="ordered locus">MJ1329</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64465">
        <property type="entry name" value="H64465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870846.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1329"/>
    <dbReference type="MEROPS" id="M24.035"/>
    <dbReference type="EnsemblBacteria" id="AAB99338">
        <property type="protein sequence ID" value="AAB99338"/>
        <property type="gene ID" value="MJ_1329"/>
    </dbReference>
    <dbReference type="GeneID" id="1452231"/>
    <dbReference type="KEGG" id="mja:MJ_1329"/>
    <dbReference type="eggNOG" id="arCOG01001">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_0_2"/>
    <dbReference type="InParanoid" id="Q58725"/>
    <dbReference type="OMA" id="NEIAAHY"/>
    <dbReference type="OrthoDB" id="82853at2157"/>
    <dbReference type="PhylomeDB" id="Q58725"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035551">
        <property type="term" value="P:protein initiator methionine removal involved in protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148975" description="Methionine aminopeptidase">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <sequence length="294" mass="32950" checksum="AA28D37E2BF34186" modified="1997-11-01" version="1">MEIEGYEKIIEAGKIASKVREEAVKLIXPGVKLLEVAEFVENRIRELGGEPAFPCNISINEIAAHYTPKLNDNLEFKDDDVVKLDLGAHVDGYIADTAITVDLSNSYKDLVKASEDALYTVIKEINPPMNIGEMGKIIQEVIESYGYKPISNLSGHVMHRYELHTGISIPNVYERTNQYIDVGDLVAIEPFATDGFGMVKDGNLGNIYKFLAKRPIRLPQARKLLDVISKNYPYLPFAERWVLKNESERLALNSLIRASCIYGYPILKERENGIVGQAEHTILITENGVEITTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58741</accession>
    <name>Y1345_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TPR repeat-containing protein MJ1345</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1345</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Trends Biochem. Sci." volume="23" first="245" last="247">
            <title>Tetratrico-peptide-repeat proteins in the archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Kyrpides N.C."/>
                <person name="Woese C.R."/>
            </authorList>
            <dbReference type="PubMed" id="9697413"/>
            <dbReference type="DOI" id="10.1016/S0968-0004(98)01228-6"/>
        </citation>
        <scope>DISCUSSION OF SEQUENCE</scope>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64467">
        <property type="entry name" value="H64467"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58741"/>
    <dbReference type="SMR" id="Q58741"/>
    <dbReference type="STRING" id="243232.MJ_1345"/>
    <dbReference type="EnsemblBacteria" id="AAB99354">
        <property type="protein sequence ID" value="AAB99354"/>
        <property type="gene ID" value="MJ_1345"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1345"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003728_2_0_2"/>
    <dbReference type="InParanoid" id="Q58741"/>
    <dbReference type="OMA" id="ACYDRAV"/>
    <dbReference type="PhylomeDB" id="Q58741"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0802">TPR repeat</keyword>
    <feature type="chain" id="PRO_0000106460" description="TPR repeat-containing protein MJ1345">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 1">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 2">
        <location>
            <begin position="46"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 3">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 4">
        <location>
            <begin position="114"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 5">
        <location>
            <begin position="147"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 6">
        <location>
            <begin position="182"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 7">
        <location>
            <begin position="215"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 8">
        <location>
            <begin position="249"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 9">
        <location>
            <begin position="284"/>
            <end position="313"/>
        </location>
    </feature>
    <sequence length="314" mass="36518" checksum="F58D35315E854E12" modified="1996-11-01" version="1">MGEGVNMEIYNESILWDEYFDALEKRNYEKALLLIDKILEVRESPDVYVRKARILRTLGENDKALEYFDKALKLKPKYILANFLKGALLVSLGKLEEAKEVFLKLCRLEKSDLPVKYVTAFILKKLGEYDYALKIIDKILKKYPKSAIAWAEKGEILYREGKLKKSLECFDNALKINPKDCQSLLYKGEILFKLGRYGEALKCLKKVFERNNKDIRALMYIIQILIYLGRLNQALEYTKKALKLNPDDPLLYLYKGIILNKLGKYNEAIKYFDKVLEINPNIPDAWNGKAIALEKLGKINEAIECYNRALDIYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58742</accession>
    <name>QUEC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="1">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeosine biosynthesis protein QueC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">PreQ(0) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queC</name>
        <name type="ordered locus">MJ1347</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7-carboxy-7-deazaguanine + ATP + NH4(+) = 7-cyano-7-deazaguanine + ADP + H(+) + H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QueC family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99356" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99356.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64468">
        <property type="entry name" value="B64468"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496801.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58742"/>
    <dbReference type="SMR" id="Q58742"/>
    <dbReference type="STRING" id="243232.MJ_1347"/>
    <dbReference type="PRIDE" id="Q58742"/>
    <dbReference type="DNASU" id="1452250"/>
    <dbReference type="EnsemblBacteria" id="AAB99356">
        <property type="protein sequence ID" value="AAB99356"/>
        <property type="gene ID" value="MJ_1347"/>
    </dbReference>
    <dbReference type="GeneID" id="1452250"/>
    <dbReference type="KEGG" id="mja:MJ_1347"/>
    <dbReference type="eggNOG" id="arCOG00039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081854_1_0_2"/>
    <dbReference type="InParanoid" id="Q58742"/>
    <dbReference type="OMA" id="CESCMRR"/>
    <dbReference type="OrthoDB" id="90765at2157"/>
    <dbReference type="PhylomeDB" id="Q58742"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="PTHR42914"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00364">
        <property type="entry name" value="TIGR00364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000107287" description="7-cyano-7-deazaguanine synthase">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="232" mass="26029" checksum="CEB27A7BC62987D6" modified="2006-07-25" version="2">MKAITVLSGGLDSTVVTLIAKDLGYEVTAITFNYGQRAAKREINSAKKICEILGIEHIVVDLPFVKQFGKSSLITEKEIPTLKMEELDSEKAYETMKAVWVPARNVIMFGIASGFAEALDAEKIFIGINKEEGVTFPDNTIEFVEAFNKVLEYGTLNKVKIEAPLYDKTKEEIVKLGAELEKKLGVEVLKYSYSCYHDNGEDFLHCGKCESCMRRKRAFLMAGVEDKTKYIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-13" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58743</accession>
    <name>Y1348_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1348</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1348</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Trends Biochem. Sci." volume="23" first="245" last="247">
            <title>Tetratrico-peptide-repeat proteins in the archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Kyrpides N.C."/>
                <person name="Woese C.R."/>
            </authorList>
            <dbReference type="PubMed" id="9697413"/>
            <dbReference type="DOI" id="10.1016/S0968-0004(98)01228-6"/>
        </citation>
        <scope>DISCUSSION OF SEQUENCE</scope>
    </reference>
    <comment type="caution">
        <text evidence="1">PubMed:9697413 reported that this sequence contains TPR repeats. These are not detected using our methodology.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64468">
        <property type="entry name" value="C64468"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58743"/>
    <dbReference type="STRING" id="243232.MJ_1348"/>
    <dbReference type="EnsemblBacteria" id="AAB99360">
        <property type="protein sequence ID" value="AAB99360"/>
        <property type="gene ID" value="MJ_1348"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1348"/>
    <dbReference type="eggNOG" id="arCOG09679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_838405_0_0_2"/>
    <dbReference type="OMA" id="YWLKGCE"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011716">
        <property type="entry name" value="TPR-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07720">
        <property type="entry name" value="TPR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107289" description="Uncharacterized protein MJ1348">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="336" mass="40443" checksum="41BF2F28CDB036FE" modified="1996-11-01" version="1">MRCLELNWEIMNKDSSNFLYKYWLKGCEFYNKKDYDKAIEYFSLSNKIFKENVPKCDYYGLREEAYKEILNNNIEEGIKKFDWFVEGILADGDYYNLSGELSTIASIFAKIGKLDITKNFLNSGGEWIYNDVIKDANSEDILKSVLTFDFSYEERKEILNREPHLRKLFEDNIYNFNSDFFDFMMMFFIGAGNWKRFLEVYEEFKNKIKSCQISNEIVNEIIKRFDKSMSDLLAIAHLLKENYEKCLYYVMLFKEYFDLDEFNEIEKNKINLIVDIAYNLKNGNVKKEEWLNRLNEIYKEIIKRPLPNTYKDAHNDDLLNEILDYYVLKEFIEKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58760</accession>
    <name>Y1365_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1365</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1365</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64470">
        <property type="entry name" value="D64470"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870882.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58760"/>
    <dbReference type="STRING" id="243232.MJ_1365"/>
    <dbReference type="DNASU" id="1452268"/>
    <dbReference type="EnsemblBacteria" id="AAB99373">
        <property type="protein sequence ID" value="AAB99373"/>
        <property type="gene ID" value="MJ_1365"/>
    </dbReference>
    <dbReference type="GeneID" id="1452268"/>
    <dbReference type="KEGG" id="mja:MJ_1365"/>
    <dbReference type="eggNOG" id="arCOG02142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032780_1_0_2"/>
    <dbReference type="InParanoid" id="Q58760"/>
    <dbReference type="OMA" id="WMLSYVQ"/>
    <dbReference type="OrthoDB" id="52124at2157"/>
    <dbReference type="PhylomeDB" id="Q58760"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd14726">
        <property type="entry name" value="TraB_PrgY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002816">
        <property type="entry name" value="TraB/PrgY/GumN_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005230">
        <property type="entry name" value="TraB_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046345">
        <property type="entry name" value="TraB_PrgY-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21530">
        <property type="entry name" value="PTHR21530"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01963">
        <property type="entry name" value="TraB_PrgY_gumN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00261">
        <property type="entry name" value="traB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107300" description="Uncharacterized protein MJ1365">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="397" mass="44777" checksum="1128105AFD1E8036" modified="1996-11-01" version="1">MVIILRHVRVFNGVNECDIYLIGTAHVSKDSIEEVEKIISSVSPEGIAVELDDRRFFSLITNEEKKVDLKKVLKEGNFLKFFIYLILANSQKKIGESFGIKPGSEMKKAIEIASKYGLPIYLIDRDIDITLSRLMDRMTFKEKMKIFWELLNSDEEDLELDDDLLNDMVKNPEKFIKLLKEISPTIYEVLVDERDRFMAKRLFELSKNKNSLVAVVGAGHVEGIVRYLKKLENGNDIDLMELIKVKKRKKSLKKLLTYGISLTIISIFLYMICYALNNPELLKMITFQWILFTGGLSALGVLLARGKLITALVAFLSAPITTLVPLPLAAVGTIAGLVELKYREITDKDLVGIINAESIKELLNNNLFRVLLVATLSNLGASIGVFYCLGKFIGFLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58768</accession>
    <name>Y1373_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1373</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1373</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64471">
        <property type="entry name" value="D64471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870890.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58768"/>
    <dbReference type="STRING" id="243232.MJ_1373"/>
    <dbReference type="EnsemblBacteria" id="AAB99388">
        <property type="protein sequence ID" value="AAB99388"/>
        <property type="gene ID" value="MJ_1373"/>
    </dbReference>
    <dbReference type="GeneID" id="1452276"/>
    <dbReference type="KEGG" id="mja:MJ_1373"/>
    <dbReference type="eggNOG" id="arCOG05081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138329_0_0_2"/>
    <dbReference type="OMA" id="VYPYEIV"/>
    <dbReference type="OrthoDB" id="107495at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025748">
        <property type="entry name" value="PrcB_C_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14343">
        <property type="entry name" value="PrcB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107302" description="Uncharacterized protein MJ1373">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="162" mass="18533" checksum="0579724028411CE5" modified="1996-11-01" version="1">MKKKIIILFLFTAILCSITLCGCISFEKTQIGNYNIKNNSCINSNVSKNQTIQNVSDKIVNNNTNILNTLNYEIIAYGAFGEKNRGYYYYYKDNKTIIVINLEEMPTAGYKIKIINITETANKITVYYKVIPPKEFAAMVVTYPYIKLSVNGTYNVECKEVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58806</accession>
    <name>LADH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Lactaldehyde dehydrogenase</fullName>
            <ecNumber>1.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1411</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="2836" last="2844">
            <title>Identification of lactaldehyde dehydrogenase in Methanocaldococcus jannaschii and its involvement in production of lactate for F420 biosynthesis.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="16585745"/>
            <dbReference type="DOI" id="10.1128/JB.188.8.2836-2844.2006"/>
        </citation>
        <scope>FUNCTION IN F420 BIOSYNTHESIS</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in F420 biosynthesis through the oxidation of lactaldehyde to lactate. The substrate preference order is propionaldehyde &gt; DL-lactaldehyde, DL-glyceraldehyde &gt; crotonaldehyde &gt; glycolaldehyde &gt; acetaldehyde, acrolein &gt; formaldehyde. No activity was observed towards methylglyoxal or glyceraldehyde-3-phosphate. Has a preference for NAD over NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(S)-lactaldehyde + H2O + NAD(+) = (S)-lactate + 2 H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:14277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.22"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">284 uM for propionaldehyde (at pH 7.5)</KM>
            <KM evidence="2">302 uM for DL-lactaldehyde (at pH 7.5)</KM>
            <Vmax evidence="2">1.12 umol/min/mg enzyme with propionaldehyde as substrate (at pH 7.5)</Vmax>
            <Vmax evidence="2">0.6 umol/min/mg enzyme with DL-lactaldehyde as substrate (at pH 7.5)</Vmax>
            <text>As the lactaldehyde used was a mixture of D and L isomers, the kinetic parameters do not reflect the possible stereospecificity of the enzyme.</text>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 9. The activity remained constant to pH 10.0. Below pH 9, the activity gradually declined, and the enzyme was inactive at pH 6 or below.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aldehyde dehydrogenase family.</text>
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        <property type="entry name" value="Ald_DH/histidinol_DH"/>
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        <property type="entry name" value="Aldehyde_DH_dom"/>
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            <begin position="1"/>
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    <feature type="binding site" evidence="1">
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        <ligand>
            <name>NAD(+)</name>
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    <evidence type="ECO:0000250" key="1"/>
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        <source>
            <dbReference type="PubMed" id="16585745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3PQA"/>
        </source>
    </evidence>
    <sequence length="463" mass="51136" checksum="46F265686595B6A5" modified="1998-06-01" version="2">MFIDGKWINREDMDVINPYSLEVIKKIPALSREEAKEAIDTAEKYKEVMKNLPITKRYNILMNIAKQIKEKKEELAKILAIDAGKPIKQARVEVERSIGTFKLAAFYVKEHRDEVIPSDDRLIFTRREPVGIVGAITPFNFPLNLSAHKIAPAIATGNVIVHHPSSKAPLVCIELAKIIENALKKYNVPLGVYNLLTGAGEVVGDEIVVNEKVNMISFTGSSKVGELITKKAGFKKIALELGGVNPNIVLKDADLNKAVNALIKGSFIYAGQVCISVGMILVDESIADKFIEMFVNKAKVLNVGNPLDEKTDVGPLISVEHAEWVEKVVEKAIDEGGKLLLGGKRDKALFYPTILEVDRDNILCKTETFAPVIPIIRTNEEEMIDIANSTEYGLHSAIFTNDINKSLKFAENLEFGGVVINDSSLFRQDNMPFGGVKKSGLGREGVKYAMEEMSNIKTIIISK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q58820</accession>
    <name>COMC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>L-sulfolactate dehydrogenase</fullName>
            <ecNumber>1.1.1.337</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>(R)-2-hydroxyacid dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>(R)-sulfolactate dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>L-2-hydroxycarboxylate dehydrogenase (NAD(+))</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">comC</name>
        <name type="synonym">mdh</name>
        <name type="ordered locus">MJ1425</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="3688" last="3692">
            <title>Identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea.</title>
            <authorList>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10850983"/>
            <dbReference type="DOI" id="10.1128/JB.182.13.3688-3692.2000"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of sulfopyruvate to (R)-sulfolactate much more efficiently than the reverse reaction. Also catalyzes the reduction of oxaloacetate, alpha-ketoglutarate, and to a much lower extent, KHTCA, but not pyruvate. Involved in the biosynthesis of both coenzyme M (with (R)-sulfolactate) and methanopterin (with alpha-ketoglutarate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a (2S)-2-hydroxycarboxylate + NAD(+) = a 2-oxocarboxylate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:34555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
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            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.1.337"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
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        <property type="entry name" value="H64477"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="resolution" value="2.50 A"/>
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        <property type="gene ID" value="MJ_1425"/>
    </dbReference>
    <dbReference type="GeneID" id="1452329"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:L-2-hydroxycarboxylate dehydrogenase (NAD+) activity"/>
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        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
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        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
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        <property type="entry name" value="Ldh_2"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000083824" description="L-sulfolactate dehydrogenase">
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="23"/>
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        <location>
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        <location>
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        <location>
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    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="339"/>
            <end position="341"/>
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    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="10850983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2X06"/>
        </source>
    </evidence>
    <sequence length="344" mass="37358" checksum="DD6B9086923CD298" modified="1997-11-01" version="1">MILKPENEKKLIIDVLKKFGVPEEDAKITADVFVDADLKGFTSHGIGRFPQYITALKLGNINPKPDIKIVKESPATAVIDGDLGLGQVVGKKAMELAIKKAKNVGVGVVATRNANHFGIAGYYSELAMNQDMIGITITNTEPAMAPFGGKEKILGTNPIAIAFKGNKYKFSLDMATASIARGKILEALRKKIKIPEGCAVDKDGKPTTDPAKALEGCILPFGGPKGYGLALAIEMLSAIGGAEVGTKVKGTANPEERCTKGDLFIAINPEFFMGKEEFKRKVDELLDEIKNSEPAEGFEILIPGEIEERNKMKRKDGFEIDKNLYNQLKEICNELGLNIEDYIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58826</accession>
    <name>COFH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase</fullName>
            <ecNumber evidence="3 4 5">2.5.1.147</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>FO synthase subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cofH</name>
        <name type="ordered locus">MJ1431</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Arch. Microbiol." volume="180" first="455" last="464">
            <title>Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F(420) biosynthesis.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="14593448"/>
            <dbReference type="DOI" id="10.1007/s00203-003-0614-8"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2012" name="J. Am. Chem. Soc." volume="134" first="18173" last="18176">
            <title>Biosynthesis of F0, precursor of the F420 cofactor, requires a unique two radical-SAM domain enzyme and tyrosine as substrate.</title>
            <authorList>
                <person name="Decamps L."/>
                <person name="Philmus B."/>
                <person name="Benjdia A."/>
                <person name="White R."/>
                <person name="Begley T.P."/>
                <person name="Berteau O."/>
            </authorList>
            <dbReference type="PubMed" id="23072415"/>
            <dbReference type="DOI" id="10.1021/ja307762b"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2015" name="J. Am. Chem. Soc." volume="137" first="5406" last="5413">
            <title>Biosynthetic versatility and coordinated action of 5'-deoxyadenosyl radicals in deazaflavin biosynthesis.</title>
            <authorList>
                <person name="Philmus B."/>
                <person name="Decamps L."/>
                <person name="Berteau O."/>
                <person name="Begley T.P."/>
            </authorList>
            <dbReference type="PubMed" id="25781338"/>
            <dbReference type="DOI" id="10.1021/ja513287k"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4 5">Catalyzes the radical-mediated synthesis of 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D-ribitylamino)uracil and L-tyrosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4 5">
            <text>5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 2-iminoacetate + 5'-deoxyadenosine + 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:55200"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="2.5.1.147"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text>Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text>Consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. CofH family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="F64478"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58826"/>
    <dbReference type="SMR" id="Q58826"/>
    <dbReference type="STRING" id="243232.MJ_1431"/>
    <dbReference type="PRIDE" id="Q58826"/>
    <dbReference type="DNASU" id="1452335"/>
    <dbReference type="EnsemblBacteria" id="AAB99441">
        <property type="protein sequence ID" value="AAB99441"/>
        <property type="gene ID" value="MJ_1431"/>
    </dbReference>
    <dbReference type="GeneID" id="1452335"/>
    <dbReference type="KEGG" id="mja:MJ_1431"/>
    <dbReference type="eggNOG" id="arCOG00656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040406_1_1_2"/>
    <dbReference type="InParanoid" id="Q58826"/>
    <dbReference type="OMA" id="ATMMFGS"/>
    <dbReference type="OrthoDB" id="26917at2157"/>
    <dbReference type="PhylomeDB" id="Q58826"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-12179"/>
    <dbReference type="BRENDA" id="2.5.1.147">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01612">
        <property type="entry name" value="FO_synth_sub2"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019940">
        <property type="entry name" value="CofH_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00293">
        <property type="entry name" value="((2_3_4_5-tetrahydroxypentyl)a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03551">
        <property type="entry name" value="F420_cofH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00423">
        <property type="entry name" value="TIGR00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141718" description="5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="45"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="14593448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="23072415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="25781338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="359" mass="40806" checksum="10B91CA7D9E18E13" modified="1996-11-01" version="1">MDPNKFREKEISKKEALELFEDNEIIFELFKFADSLRREEVGDIVTYVVNRNINFTNICVGNCRFCAFRANENDKHAYFLDIDEIAKRAVEAKKFGCTEVCIQGGLHPKIDTYYQAEILKAVHEATKPYGDIHIHAFSPMEVYFGAENAGLDIKEALKILKENGLNSMPGTAAEILDDDIRAELCPNKIKTKEWIYIIKEAHKLGIPTTATMMYGHIEEYKHWVNHLFIIKEIQEETNGFTEFVPLSFMHKYAPIYKEGKAKAGATGIEDLKVFAVSRIIFKGLIKNIQASWVKLGKKMVQVALRCGANDVGGTLIEESISRSAGAEHGVYMSVEEIRDMIKRVGLIPKERTTLYKILE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58831</accession>
    <name>Y1436_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1436</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1436</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH1236.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64479">
        <property type="entry name" value="C64479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870954.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58831"/>
    <dbReference type="SMR" id="Q58831"/>
    <dbReference type="STRING" id="243232.MJ_1436"/>
    <dbReference type="EnsemblBacteria" id="AAB99445">
        <property type="protein sequence ID" value="AAB99445"/>
        <property type="gene ID" value="MJ_1436"/>
    </dbReference>
    <dbReference type="GeneID" id="1452340"/>
    <dbReference type="KEGG" id="mja:MJ_1436"/>
    <dbReference type="eggNOG" id="arCOG04876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_162437_0_0_2"/>
    <dbReference type="OMA" id="KPFPYRN"/>
    <dbReference type="OrthoDB" id="113300at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019597">
        <property type="entry name" value="Energy-convert_hydgase-B_suP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10622">
        <property type="entry name" value="Ehbp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107327" description="Uncharacterized protein MJ1436">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="97" mass="11265" checksum="FD1065C09E68AB4D" modified="1996-11-01" version="1">MPKMVLLPRMTMALGGYIRETTFPYEEDDEVKPFPYRNVIVGNPTDEPIKIEVPAYNEGWIERHKKLGLIVVPVNEDDDFVGLFQMVKEKVKNAKRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58905</accession>
    <name>TYW3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA wyosine derivatives biosynthesis protein Taw3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">taw3</name>
        <name type="ordered locus">MJ1510</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="Mol. Biol. Evol." volume="27" first="2062" last="2077">
            <title>Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea.</title>
            <authorList>
                <person name="de Crecy-Lagard V."/>
                <person name="Brochier-Armanet C."/>
                <person name="Urbonavicius J."/>
                <person name="Fernandez B."/>
                <person name="Phillips G."/>
                <person name="Lyons B."/>
                <person name="Noma A."/>
                <person name="Alvarez S."/>
                <person name="Droogmans L."/>
                <person name="Armengaud J."/>
                <person name="Grosjean H."/>
            </authorList>
            <dbReference type="PubMed" id="20382657"/>
            <dbReference type="DOI" id="10.1093/molbev/msq096"/>
        </citation>
        <scope>GENE NAME</scope>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TYW3 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64488">
        <property type="entry name" value="E64488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871033.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58905"/>
    <dbReference type="SMR" id="Q58905"/>
    <dbReference type="STRING" id="243232.MJ_1510"/>
    <dbReference type="EnsemblBacteria" id="AAB99529">
        <property type="protein sequence ID" value="AAB99529"/>
        <property type="gene ID" value="MJ_1510"/>
    </dbReference>
    <dbReference type="GeneID" id="1452417"/>
    <dbReference type="KEGG" id="mja:MJ_1510"/>
    <dbReference type="eggNOG" id="arCOG04156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047426_2_0_2"/>
    <dbReference type="InParanoid" id="Q58905"/>
    <dbReference type="OMA" id="GKWHHYA"/>
    <dbReference type="OrthoDB" id="118297at2157"/>
    <dbReference type="PhylomeDB" id="Q58905"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00266">
        <property type="entry name" value="TYW3_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022908">
        <property type="entry name" value="Taw3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSF111278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000157093" description="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00266"/>
        </source>
    </evidence>
    <sequence length="193" mass="22401" checksum="D8E149EC7B4A51C9" modified="1996-11-01" version="1">MGFLEDKKRTLMNLELAIREGLVDEEIIPILNKINEIDNYYTTSSCIGRVGIMEIPKDKNPKLYSRWLGKWHHYASYDELFNALKNKKEGYIVFVMNSPILHIACKDIESAKKMLELAIHSGLKASSIKSISDKRVIVEILTTYKVDTPIGEDGEIFVDNNYLKFLLDYSNSKLKRAREILMRWANRLDELKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58920</accession>
    <name>AGLB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber>2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Oligosaccharyl transferase</fullName>
            <shortName>OST</shortName>
            <shortName>OTase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">aglB</name>
        <name type="ordered locus">MJ1525</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Oligosaccharyl transferase (OST) that catalyzes the initial transfer of a defined glycan (ManNAcGlc-2,3-diNAcAGlcNAc in Methanococci) from the lipid carrier dolichol-monophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. Involved in the assembly of an N-linked disaccharide that decorates the S-layer glycoprotein and flagellins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cell surface structure biogenesis; S-layer biogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2">Despite low primary sequence conservation between eukaryotic catalytic subunits and bacterial and archaeal single subunit OSTs (ssOST), structural comparison revealed several common motifs at spatially equivalent positions, like the DXD motif 1 on the external loop 1 and the DXD motif 2 on the external loop 2 involved in binding of the metal ion cofactor and the carboxamide group of the acceptor asparagine, the conserved Glu residue of the TIXE/SVSE motif on the external loop 5 involved in catalysis, as well as the WWDYG and the DK/MI motifs in the globular domain that define the binding pocket for the +2 Ser/Thr of the acceptor sequon. In bacterial ssOSTs, an Arg residue was found to interact with a negatively charged side chain at the -2 position of the sequon. This Arg is conserved in bacterial enzymes and correlates with an extended sequon requirement (Asp-X-Asn-X-Ser/Thr) for bacterial N-glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64490">
        <property type="entry name" value="D64490"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58920"/>
    <dbReference type="STRING" id="243232.MJ_1525"/>
    <dbReference type="CAZy" id="GT66">
        <property type="family name" value="Glycosyltransferase Family 66"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99543">
        <property type="protein sequence ID" value="AAB99543"/>
        <property type="gene ID" value="MJ_1525"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1525"/>
    <dbReference type="eggNOG" id="arCOG02044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018380_0_0_2"/>
    <dbReference type="InParanoid" id="Q58920"/>
    <dbReference type="OMA" id="WDNGHIY"/>
    <dbReference type="BRENDA" id="2.4.99.18">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="UniPathway" id="UPA00977"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006486">
        <property type="term" value="P:protein glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045232">
        <property type="term" value="P:S-layer organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="PTHR13872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107392" description="Dolichyl-phosphooligosaccharide-protein glycotransferase">
        <location>
            <begin position="1"/>
            <end position="933"/>
        </location>
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    <feature type="topological domain" description="Cytoplasmic" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="6">
        <location>
            <begin position="44"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="6">
        <location>
            <begin position="168"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="6">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="6">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="6">
        <location>
            <begin position="258"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="6">
        <location>
            <begin position="285"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="6">
        <location>
            <position position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="6">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="362"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="6">
        <location>
            <begin position="383"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="6">
        <location>
            <begin position="449"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="455"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="6">
        <location>
            <begin position="476"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="480"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="6">
        <location>
            <begin position="501"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="582"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="6">
        <location>
            <begin position="603"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interacts with target acceptor peptide in protein substrate" evidence="1">
        <location>
            <begin position="629"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DXD motif 1" evidence="1">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DXD motif 2" evidence="1">
        <location>
            <begin position="200"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TIXE motif" evidence="2">
        <location>
            <begin position="410"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="WWDYG motif" evidence="3">
        <location>
            <begin position="629"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="MI motif" evidence="3">
        <location>
            <begin position="756"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="413"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>a glycophospholipid</name>
            <dbReference type="ChEBI" id="CHEBI:24397"/>
            <note>archaeal dolichyl phosphooligosaccharide</note>
        </ligand>
    </feature>
    <feature type="site" description="Interacts with target acceptor peptide in protein substrate" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalytic activity" evidence="1">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with target acceptor peptide in protein substrate" evidence="1">
        <location>
            <position position="413"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with target acceptor peptide in protein substrate" evidence="1">
        <location>
            <position position="759"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="B9KDD4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O29867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="O29918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="4">
        <source>
            <dbReference type="UniProtKB" id="Q2EMT4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5"/>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="933" mass="103964" checksum="F3B555913A77379C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58935</accession>
    <name>Y1540_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1540</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1540</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64492">
        <property type="entry name" value="C64492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871064.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58935"/>
    <dbReference type="STRING" id="243232.MJ_1540"/>
    <dbReference type="EnsemblBacteria" id="AAB99569">
        <property type="protein sequence ID" value="AAB99569"/>
        <property type="gene ID" value="MJ_1540"/>
    </dbReference>
    <dbReference type="GeneID" id="1452448"/>
    <dbReference type="KEGG" id="mja:MJ_1540"/>
    <dbReference type="eggNOG" id="arCOG08298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881070_0_0_2"/>
    <dbReference type="OMA" id="NIFQFSK"/>
    <dbReference type="OrthoDB" id="111249at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107398" description="Uncharacterized protein MJ1540">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <sequence length="136" mass="15633" checksum="0590C8C629EAFE04" modified="1996-11-01" version="1">MLKELNYLFLFQRLIKNGSLEIALTNIFSLGISRDYITINIENIKTLKEIMEAVSSKSANKKLKSFLEKIEDLKIFLSEASKIAEILVNNRKTFILKYKGKEVLIVGYDARGGIFLKNIKITDKLALIKMLNEFKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q58979</accession>
    <name>Y1584_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1584</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1584</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64497">
        <property type="entry name" value="G64497"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58979"/>
    <dbReference type="STRING" id="243232.MJ_1584"/>
    <dbReference type="EnsemblBacteria" id="AAB99608">
        <property type="protein sequence ID" value="AAB99608"/>
        <property type="gene ID" value="MJ_1584"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1584"/>
    <dbReference type="HOGENOM" id="CLU_1727254_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107427" description="Uncharacterized protein MJ1584">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <sequence length="151" mass="17270" checksum="EC712881545F28F4" modified="1996-11-01" version="1">MEIGNKVMEMKNDISKWKLIKTLYKVLKLKCWYDDENYAWSGGLSAYADGLRLLSKVGLFKIESEYCRSVTGKFKELKASAVNLNAVDEDELIETLYRVLRQSCIMNIDDNIGESFGKKVYASGLRLLSKLGLFEIESEQGDYIIGKFKEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58984</accession>
    <name>Y1589_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1589</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1589</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0871, MJ0880 and MJ1556.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64498">
        <property type="entry name" value="D64498"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58984"/>
    <dbReference type="STRING" id="243232.MJ_1589"/>
    <dbReference type="EnsemblBacteria" id="AAB99617">
        <property type="protein sequence ID" value="AAB99617"/>
        <property type="gene ID" value="MJ_1589"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1589"/>
    <dbReference type="eggNOG" id="arCOG00360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100473_0_0_2"/>
    <dbReference type="OMA" id="FGIHIIL"/>
    <dbReference type="PhylomeDB" id="Q58984"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="PTHR38139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107430" description="Uncharacterized protein MJ1589">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="241" mass="27083" checksum="45C5AFD0496723EF" modified="1996-11-01" version="1">MMMALQIFIKILPIMFFGILLANLMCHLNILYKLQKYIKNKYFPIIAVFFVSSTSGSFLLKNLLKKGEISEENLLPIYFLGMFVFGIHIILFYAIPMATSLGWYVGGIYVLIKFLVTCNYLIISVLMLKKRKYNIDIEFKSKSEGLYGAIRDTFKQYFRVLTSFVPSVLIITYLIEHGLLDIVEDFAGSLLNALNLSPTILVIVLTGLATISGAIGIASGLLDENILSPNEVLFSLFLAGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58997</accession>
    <name>DHOM_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Homoserine dehydrogenase</fullName>
            <shortName>HDH</shortName>
            <ecNumber>1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hom</name>
        <name type="ordered locus">MJ1602</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>L-homoserine + NADP(+) = H(+) + L-aspartate 4-semialdehyde + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15761"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>L-homoserine + NAD(+) = H(+) + L-aspartate 4-semialdehyde + NADH</text>
            <dbReference type="Rhea" id="RHEA:15757"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.1.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64500">
        <property type="entry name" value="A64500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871127.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58997"/>
    <dbReference type="SMR" id="Q58997"/>
    <dbReference type="STRING" id="243232.MJ_1602"/>
    <dbReference type="PRIDE" id="Q58997"/>
    <dbReference type="EnsemblBacteria" id="AAB99622">
        <property type="protein sequence ID" value="AAB99622"/>
        <property type="gene ID" value="MJ_1602"/>
    </dbReference>
    <dbReference type="GeneID" id="1452511"/>
    <dbReference type="KEGG" id="mja:MJ_1602"/>
    <dbReference type="eggNOG" id="arCOG01351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_1_2_2"/>
    <dbReference type="InParanoid" id="Q58997"/>
    <dbReference type="OMA" id="LMFYGPG"/>
    <dbReference type="OrthoDB" id="102435at2157"/>
    <dbReference type="PhylomeDB" id="Q58997"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="chain" id="PRO_0000066705" description="Homoserine dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="336" mass="36462" checksum="EDAEFA1998439B48" modified="1996-11-01" version="1">MDIIIVGFGAIGKGIAKVLYDKKDYLKKNYEEFKVVAITDSSGAAIDEDGLDLLKAIEVKEKTGKIKNYPEKGREMSSIDVIKEVDADVVVEVTPSNLETGDPAKTHILESFKNKKHVVTANKGPLALCYKELIEEAKKHGVIFRHEASVGGAMPIINLAKETLAGNEILSIRGILNGTTNYILTKMEKEGLDFETALKEAKELGIAETDPTQDIEGLDTAAKIVILANSIMGMNKTIKDVKVKGISRITPEALFLANKRGYTIKLIGQIKDGYLIVEPMLVPIDSPLNVKGTLNVAMFETDLAKEVVVVGRGAGPIETASAILSDLIHIYNSTKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q59026</accession>
    <name>Y1632_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative glycyl-radical enzyme activating enzyme MJ1632</fullName>
            <shortName evidence="3">GRE activating enzyme MJ1632</shortName>
            <ecNumber evidence="1">1.97.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1632</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:61976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:14480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15993"/>
            <dbReference type="Rhea" id="RHEA-COMP:15994"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29947"/>
            <dbReference type="ChEBI" id="CHEBI:32722"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:140311"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the organic radical-activating enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.97.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64503">
        <property type="entry name" value="F64503"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59026"/>
    <dbReference type="SMR" id="Q59026"/>
    <dbReference type="STRING" id="243232.MJ_1632"/>
    <dbReference type="EnsemblBacteria" id="AAB99653">
        <property type="protein sequence ID" value="AAB99653"/>
        <property type="gene ID" value="MJ_1632"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1632"/>
    <dbReference type="eggNOG" id="arCOG00954">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1217571_0_0_2"/>
    <dbReference type="InParanoid" id="Q59026"/>
    <dbReference type="OMA" id="LTPWDHH"/>
    <dbReference type="PhylomeDB" id="Q59026"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001989">
        <property type="entry name" value="Radical_activat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01087">
        <property type="entry name" value="RADICAL_ACTIVATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000200546" description="Putative glycyl-radical enzyme activating enzyme MJ1632">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="30"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0A9N4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="255" mass="29795" checksum="D1F2EC1BA31D39A7" modified="1996-11-01" version="1">MLNTLMLREWLRSMLRSCISKIFGDLMLISHISLSDKITLLTYGCNFKCKYCFFKPLSCKKYSVDEILNKILEVNENYKLDKILIAGGEPTLQNDLSELTKLLKDEGFYLMLSTNGYYLKDMLDKLEVDEIHIDLKAYDENKHIYLTSCSNKKVLDCISYIGKYRDEFNFKVEIDTVLIPNIVDLDEIEKIAKFLSNWDLPYRITGYVKYNNNLNAEKPDEDKILKAKEIALKYLSNVSCSLDFKRHKKSKKVII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q59028</accession>
    <name>MPTE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="1">HPPK</shortName>
            <ecNumber evidence="1 2">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="1 3">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1 3">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="1">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">mptE</name>
        <name type="ordered locus">MJ1634</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="ACS Chem. Biol." volume="7" first="1807" last="1816">
            <title>Comparative genomics guided discovery of two missing archaeal enzyme families involved in the biosynthesis of the pterin moiety of tetrahydromethanopterin and tetrahydrofolate.</title>
            <authorList>
                <person name="Crecy-Lagard V.D."/>
                <person name="Phillips G."/>
                <person name="Grochowski L.L."/>
                <person name="Yacoubi B.E."/>
                <person name="Jenney F."/>
                <person name="Adams M.W."/>
                <person name="Murzin A.G."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="22931285"/>
            <dbReference type="DOI" id="10.1021/cb300342u"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>GENE NAME</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64503">
        <property type="entry name" value="H64503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871158.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59028"/>
    <dbReference type="STRING" id="243232.MJ_1634"/>
    <dbReference type="EnsemblBacteria" id="AAB99656">
        <property type="protein sequence ID" value="AAB99656"/>
        <property type="gene ID" value="MJ_1634"/>
    </dbReference>
    <dbReference type="GeneID" id="1452543"/>
    <dbReference type="KEGG" id="mja:MJ_1634"/>
    <dbReference type="eggNOG" id="arCOG04303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093043_0_0_2"/>
    <dbReference type="InParanoid" id="Q59028"/>
    <dbReference type="OMA" id="HAHGDNM"/>
    <dbReference type="OrthoDB" id="105604at2157"/>
    <dbReference type="PhylomeDB" id="Q59028"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-17934"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="PTHR39648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MAF_flag10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="SSF63999"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107449" description="6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22931285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="22931285"/>
        </source>
    </evidence>
    <sequence length="241" mass="27581" checksum="7E7F2EBB4AC5DBAA" modified="1996-11-01" version="1">MDMKEWEIFYNKIMEDFGFDKDKDVESAVILNNILENANTIPVDKLKDIIEGREVFIFGAGPSIKKHINILKELREINYKNPIIVADGACKAFLEENIIPDIIVSDLDGDLEALFECNRKGSIIVVHAHGDNIEKIKKYVPKLKNVVGSCQIPNYKELNLRNVINFGGFTDGDRCCFLAYHFKAKKLILGGMDFGIYITKYSRPNIKEDIAIGDEIKIKKLEYAKTLINYLKDKIEIEFLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q59038</accession>
    <name>Y1644_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1644</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1644</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64505">
        <property type="entry name" value="B64505"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59038"/>
    <dbReference type="STRING" id="243232.MJ_1644"/>
    <dbReference type="DNASU" id="1452553"/>
    <dbReference type="EnsemblBacteria" id="AAB99675">
        <property type="protein sequence ID" value="AAB99675"/>
        <property type="gene ID" value="MJ_1644"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1644"/>
    <dbReference type="eggNOG" id="arCOG01644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105758_1_1_2"/>
    <dbReference type="InParanoid" id="Q59038"/>
    <dbReference type="OMA" id="LEIVAFF"/>
    <dbReference type="PhylomeDB" id="Q59038"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010397">
        <property type="entry name" value="DUF996"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06195">
        <property type="entry name" value="DUF996"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019678">
        <property type="entry name" value="UCP019678"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107456" description="Uncharacterized protein MJ1644">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="177" mass="19461" checksum="A94D57AF68E2804C" modified="1996-11-01" version="1">MIKMELKEAKYLGGIGAVLNLVSYAVGGILAIAGYVLILLALNKISKIFNDDEVFKKYLYGVVLWIIAVLIVIFAVGISFVSLSFIPLDYGLTAMSSFLVGVILFYILSVIGGYFIKKSYEKVSYYTGVDSFRICGLLYFIGTLLLIVIVGIIVIIVAQILEIVAYFSLPDDLKSEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q59056</accession>
    <name>Y1662_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ1662</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1662</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871186.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59056"/>
    <dbReference type="STRING" id="243232.MJ_1662"/>
    <dbReference type="PRIDE" id="Q59056"/>
    <dbReference type="EnsemblBacteria" id="AAB99680">
        <property type="protein sequence ID" value="AAB99680"/>
        <property type="gene ID" value="MJ_1662"/>
    </dbReference>
    <dbReference type="GeneID" id="1452571"/>
    <dbReference type="KEGG" id="mja:MJ_1662"/>
    <dbReference type="eggNOG" id="arCOG00185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="InParanoid" id="Q59056"/>
    <dbReference type="OMA" id="DPMTRLD"/>
    <dbReference type="OrthoDB" id="6733at2157"/>
    <dbReference type="PhylomeDB" id="Q59056"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019700">
        <property type="term" value="P:organic phosphonate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093227" description="Uncharacterized ABC transporter ATP-binding protein MJ1662">
        <location>
            <begin position="1"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 1" evidence="1">
        <location>
            <begin position="4"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 2" evidence="1">
        <location>
            <begin position="255"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="292"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="555" mass="63040" checksum="18BBF0BB89E037E1" modified="1998-06-01" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q60182</accession>
    <name>GLNA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glutamine synthetase</fullName>
            <shortName evidence="9">GS</shortName>
            <ecNumber evidence="8">6.3.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Glutamate--ammonia ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamine synthetase I alpha</fullName>
            <shortName evidence="9">GSI alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glnA</name>
        <name type="ordered locus">MJ1346</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Appl. Environ. Microbiol." volume="67" first="4458" last="4463">
            <title>beta-Glutamate as a substrate for glutamine synthetase.</title>
            <authorList>
                <person name="Robinson P."/>
                <person name="Neelon K."/>
                <person name="Schreier H.J."/>
                <person name="Roberts M.F."/>
            </authorList>
            <dbReference type="PubMed" id="11571143"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probably involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Beta-glutamate is a much poorer substrate than alpha-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="10">Feedback inhibited by glycine and alanine, and inhibited by low concentrations of methionine sulfoximine.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="8">23 mM for alpha-glutamine (at 37 degrees Celsius)</KM>
            <KM evidence="8">40 mM for alpha-glutamine (at 50 degrees Celsius)</KM>
            <KM evidence="8">58 mM for alpha-glutamate (at 60 degrees Celsius)</KM>
            <Vmax evidence="8">26.7 umol/min/mg enzyme toward alpha-glutamine (at 50 degrees Celsius)</Vmax>
            <Vmax evidence="8">3.5 umol/min/mg enzyme toward alpha-glutamine (at 37 degrees Celsius)</Vmax>
            <Vmax evidence="8">1.3 umol/min/mg enzyme toward alpha-glutamate (at 60 degrees Celsius)</Vmax>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomer of 12 subunits arranged in the form of two hexagons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="8"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64468">
        <property type="entry name" value="A64468"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60182"/>
    <dbReference type="SMR" id="Q60182"/>
    <dbReference type="STRING" id="243232.MJ_1346"/>
    <dbReference type="EnsemblBacteria" id="AAB99355">
        <property type="protein sequence ID" value="AAB99355"/>
        <property type="gene ID" value="MJ_1346"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1346"/>
    <dbReference type="eggNOG" id="arCOG01909">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017290_1_3_2"/>
    <dbReference type="InParanoid" id="Q60182"/>
    <dbReference type="OMA" id="TFYTHPE"/>
    <dbReference type="PhylomeDB" id="Q60182"/>
    <dbReference type="BRENDA" id="6.3.1.2">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamate-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_b-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="SSF54368"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153204" description="Glutamine synthetase">
        <location>
            <begin position="1"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="GS beta-grasp" evidence="6">
        <location>
            <begin position="19"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="7">
        <location>
            <begin position="118"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="250"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="257"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0A1P6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P12425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="P77961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="4">
        <source>
            <dbReference type="UniProtKB" id="P9WN39"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="5">
        <source>
            <dbReference type="UniProtKB" id="Q9HH09"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="8">
        <source>
            <dbReference type="PubMed" id="11571143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="9">
        <source>
            <dbReference type="PubMed" id="11571143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="10">
        <source>
            <dbReference type="PubMed" id="11571143"/>
        </source>
    </evidence>
    <sequence length="454" mass="51399" checksum="3222CBA464D18120" modified="1997-11-01" version="1">MHWGMEMNVEQAIEYVKKNNVKFIRFQFVDILGFPKNVAYPVKAGEKGIEELREIFENGVWFDGSSITGFVGIEESDMLLKPDLSTLSVLPWRPEEKSVARVICDVYKDEKTPFEGDPRSRLKAILEELKKEMNGEYFVGPEPEFFLLKRDPHNPHRWVPADDGGYFDVEPLDDAPDIRRDIVLALENLGFHVEASHHEVAPGQHEVDFKFDNALKTADSVITFKMTIKNIAKKHGLKATFMPKPFFGMNGNGMHCHQSVWFNGEPSFYDPEGPYNGLSETCLSYIAGILSHAKALVAITNPTVNSYKRLVPGYEAPVNIAWANKNRSAIIRVPAARGKATRIEFRAPDPTCNPYLAFACMLAAGLDGIKKKMTAPEPVERNIFKMSEEEKKQLGIESVPANLAAALDELECDEVLQKALGKHIYENYMEIKRAEWDDFRTAVTDWETGKYLIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q60328</accession>
    <name>Y012_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TnpB-like protein MJ0012</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0012</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64301">
        <property type="entry name" value="D64301"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60328"/>
    <dbReference type="SMR" id="Q60328"/>
    <dbReference type="InParanoid" id="Q60328"/>
    <dbReference type="PhylomeDB" id="Q60328"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0814">Transposable element</keyword>
    <keyword id="KW-0815">Transposition</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000106652" description="TnpB-like protein MJ0012">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q7DF80"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="208" mass="24347" checksum="4C824F93015561B4" modified="1997-11-01" version="1">MKKYWNRRKNRVEDFINKLTSQLSKLFPDAIFIFEDLDKFNMYDKNSNFNRNLDRTNWRKIAKKLEYKSVVLYVNPHYTSKTCPVCGSKMKSQEGQVVKCDKCGIFDRQFVRCYNIFKRGVELAKKLLGGVGVPVAGAEVDDLLSNEPRGELRLVKPNPNVEAKLPVRKSNRRFELQNPKDFVQIFDFPLMVYTVDLNGKYLKIYNCP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q60331</accession>
    <name>GATD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="1">Glu-ADT subunit D</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatD</name>
        <name type="ordered locus">MJ0020</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64302">
        <property type="entry name" value="D64302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869512.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60331"/>
    <dbReference type="SMR" id="Q60331"/>
    <dbReference type="STRING" id="243232.MJ_0020"/>
    <dbReference type="EnsemblBacteria" id="AAB97996">
        <property type="protein sequence ID" value="AAB97996"/>
        <property type="gene ID" value="MJ_0020"/>
    </dbReference>
    <dbReference type="GeneID" id="1450858"/>
    <dbReference type="KEGG" id="mja:MJ_0020"/>
    <dbReference type="eggNOG" id="arCOG01924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_2_1_2"/>
    <dbReference type="InParanoid" id="Q60331"/>
    <dbReference type="OMA" id="RKNHTSR"/>
    <dbReference type="OrthoDB" id="61613at2157"/>
    <dbReference type="PhylomeDB" id="Q60331"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="SSF141300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="SSF53774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140052" description="Glutamyl-tRNA(Gln) amidotransferase subunit D">
        <location>
            <begin position="1"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase" evidence="2">
        <location>
            <begin position="74"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01068"/>
        </source>
    </evidence>
    <sequence length="417" mass="46344" checksum="27FFF41E62F41EE3" modified="1997-11-01" version="1">MNVGDVIRVETDKGIFEGILLPSTNENIITIKMKNGYNVGILKENVKNIEIIAKGEKPKYELPPLNIEKNEKLKTISILSTGGTVASKVDYKTGAVHPSFTADDLIRAVPELLDIANIKGRAVMNILSENMKPEYWRKIAEEIKKEIEEGADGIVIAHGTDTMSYTASALSFMVKADVPIILVGAQRSSDRPSSDAALNLISAVLAAREPIKGVYVVMHGESGDTFCYLHKGVKVRKCHSSRRDAFKSINSIPVAKINPFTKEIIYLQEVEKSDNSKKVEINTNLEEKVALIKVYPGMDGEIIRFYVDKEYKGIVLEGTGLGHAPEYIFEHIKYATDKGVVVVMTTQTINGRVNMNVYSNGRELQKLGVIGCEDMPPEVALVKLMYLLGNYEPEEVKKLINKNLVGEIEYRSRFDAY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q60340</accession>
    <name>FRHG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Coenzyme F420 hydrogenase subunit gamma</fullName>
            <ecNumber>1.12.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>8-hydroxy-5-deazaflavin-reducing hydrogenase subunit gamma</fullName>
            <shortName>FRH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">frhG</name>
        <name type="ordered locus">MJ0031</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>H(+) + H2 + oxidized coenzyme F420-(gamma-L-Glu)(n) = reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:23760"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.12.98.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterocomplex of the form (alpha(1)beta(1)gamma(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64303">
        <property type="entry name" value="G64303"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60340"/>
    <dbReference type="SMR" id="Q60340"/>
    <dbReference type="STRING" id="243232.MJ_0031"/>
    <dbReference type="EnsemblBacteria" id="AAB98010">
        <property type="protein sequence ID" value="AAB98010"/>
        <property type="gene ID" value="MJ_0031"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0031"/>
    <dbReference type="eggNOG" id="arCOG02473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075477_0_0_2"/>
    <dbReference type="InParanoid" id="Q60340"/>
    <dbReference type="OMA" id="CNAENVH"/>
    <dbReference type="PhylomeDB" id="Q60340"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017681">
        <property type="entry name" value="Coenz_F420_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03294">
        <property type="entry name" value="FrhG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159232" description="Coenzyme F420 hydrogenase subunit gamma">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="169"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="198"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="230" mass="24534" checksum="6C9936D3A20ED356" modified="1997-11-01" version="1">MIKVVKVAHVQLCSCCGCLVSLADTYEKLLDVLNSIELVYCQTLADAREIPECDIALVEGSVCLDDHHSLEVAQEVRKKAKIVVALGACAATGGVTRYCKGNQLSKPVHSSFSPLTEVIKVDLAIPGCPPSPEAIVGVITAALNGDMEYLQPYAELAEKGSEACGCDVIYKVVNKSLCMGCGTCAAACPTRAIEMLDGRPNVLKELCIKCGACSVQCPRIRFPELIEKIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q60357</accession>
    <name>IF6_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation initiation factor 6</fullName>
            <shortName evidence="1">aIF-6</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eif6</name>
        <name type="ordered locus">MJ0048</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Nat. Struct. Biol." volume="7" first="1156" last="1164">
            <title>Crystal structures of ribosome anti-association factor IF6.</title>
            <authorList>
                <person name="Groft C.M."/>
                <person name="Beckmann R."/>
                <person name="Sali A."/>
                <person name="Burley S.K."/>
            </authorList>
            <dbReference type="PubMed" id="11101899"/>
            <dbReference type="DOI" id="10.1038/82017"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Binds to the 50S ribosomal subunit and prevents its association with the 30S ribosomal subunit to form the 70S initiation complex.</text>
    </comment>
    <comment type="mass spectrometry" mass="24948.0" error="6.0" method="MALDI" evidence="2"/>
    <comment type="similarity">
        <text evidence="1">Belongs to the eIF-6 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64305">
        <property type="entry name" value="H64305"/>
    </dbReference>
    <dbReference type="PDB" id="1G61">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.30 A"/>
        <property type="chains" value="A/B=1-228"/>
    </dbReference>
    <dbReference type="PDBsum" id="1G61"/>
    <dbReference type="AlphaFoldDB" id="Q60357"/>
    <dbReference type="SMR" id="Q60357"/>
    <dbReference type="STRING" id="243232.MJ_0048"/>
    <dbReference type="EnsemblBacteria" id="AAB98029">
        <property type="protein sequence ID" value="AAB98029"/>
        <property type="gene ID" value="MJ_0048"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0048"/>
    <dbReference type="eggNOG" id="arCOG04176">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071894_1_0_2"/>
    <dbReference type="InParanoid" id="Q60357"/>
    <dbReference type="OMA" id="GEDTTGP"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="IF6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="eIF_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="eIF6"/>
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        <property type="entry name" value="PTHR10784"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153746" description="Translation initiation factor 6">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
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        <location>
            <begin position="4"/>
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        <location>
            <begin position="107"/>
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    </feature>
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        <location>
            <begin position="111"/>
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        <location>
            <begin position="115"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="122"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="128"/>
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        </location>
    </feature>
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        <location>
            <begin position="137"/>
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        </location>
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        <location>
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    </feature>
    <feature type="strand" evidence="3">
        <location>
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        <location>
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        <location>
            <begin position="182"/>
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        </location>
    </feature>
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        <location>
            <begin position="190"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="200"/>
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        </location>
    </feature>
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        <location>
            <begin position="207"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11101899"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1G61"/>
        </source>
    </evidence>
    <sequence length="228" mass="24459" checksum="94DA7E11C10FCB47" modified="1997-11-01" version="1">MTMIIRKYFSGIPTIGVLALTTEEITLLPIFLDKDDVNEVSEVLETKCLQTNIGGSSLVGSLSVANKYGLLLPKIVEDEELDRIKNFLKENNLDLNVEIIKSKNTALGNLILTNDKGALISPELKDFKKDIEDSLNVEVEIGTIAELPTVGSNAVVTNKGCLTHPLVEDDELEFLKSLFKVEYIGKGTANKGTTSVGACIIANSKGAVVGGDTTGPELLIIEDALGLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q60360</accession>
    <name>Y053_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0053</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0053</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64306">
        <property type="entry name" value="E64306"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60360"/>
    <dbReference type="STRING" id="243232.MJ_0053"/>
    <dbReference type="EnsemblBacteria" id="AAB98039">
        <property type="protein sequence ID" value="AAB98039"/>
        <property type="gene ID" value="MJ_0053"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0053"/>
    <dbReference type="eggNOG" id="arCOG05019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259105_0_0_2"/>
    <dbReference type="InParanoid" id="Q60360"/>
    <dbReference type="OMA" id="CPYECQI"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030815">
        <property type="entry name" value="Arch_SelU_Cterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04569">
        <property type="entry name" value="arch_SelU_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106671" description="Uncharacterized protein MJ0053">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="17"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="227" mass="26722" checksum="A10A48D331225665" modified="1997-11-01" version="1">MGLSFNGENMIIFGLFGKTGCGKTEILNELKKHHPVIDIEEIARTRGSILGDLYHLSMRSQEEFDYLINKEIEKAKKFGYAVVEYEGRKIGGEKKLKIPELLADIKNYTYKILIDCPYECQINRLVSIYKPKNEKEKEILINKFLILKESFKKPEMIEAVDNIIELIKQDKYYEAAKLIEEKLYREHYMRNVKKIKPDLIVYNEDVKKSAKIIDEFIKKKLKEHNLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q60367</accession>
    <name>MTIP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable S-methyl-5'-thioinosine phosphorylase</fullName>
            <ecNumber evidence="1">2.4.2.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-methylthioinosine phosphorylase</fullName>
            <shortName evidence="1">MTI phosphorylase</shortName>
            <shortName evidence="1">MTIP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0060</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + S-methyl-5'-thioinosine = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:30643"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.44" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64307">
        <property type="entry name" value="D64307"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869552.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60367"/>
    <dbReference type="SMR" id="Q60367"/>
    <dbReference type="STRING" id="243232.MJ_0060"/>
    <dbReference type="EnsemblBacteria" id="AAB98042">
        <property type="protein sequence ID" value="AAB98042"/>
        <property type="gene ID" value="MJ_0060"/>
    </dbReference>
    <dbReference type="GeneID" id="1450899"/>
    <dbReference type="KEGG" id="mja:MJ_0060"/>
    <dbReference type="eggNOG" id="arCOG01327">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054456_0_2_2"/>
    <dbReference type="InParanoid" id="Q60367"/>
    <dbReference type="OMA" id="MTQCPEA"/>
    <dbReference type="OrthoDB" id="63298at2157"/>
    <dbReference type="PhylomeDB" id="Q60367"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="PTHR42679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="SSF53167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000184559" description="Probable S-methyl-5'-thioinosine phosphorylase">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="44"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="1">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <sequence length="252" mass="28664" checksum="DFD7C01615774A79" modified="1997-11-01" version="1">MIGIIGGTGIAEILKGDKEEIINTKYGKARVIIDKENEVVLLFRHGVRHNIPPHKINYRANIYALKKLGVERILAINSVGSLKEDLKPGMFFVPNDFIEFTKKREETFYDEGKVVHIDMTDPYCPELRNILKSILDKNNFSYGEGVYVCTEGPRFETKKEIAIYKNWGDVVGMTGYPEVVLARELEMCYVSLCNITNYACGISKNILTVDEVLEKIKEMENKILKVVEDFINYGFGERKCICKDALKHAVIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q60391</accession>
    <name>MCRX_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase II subunit alpha</fullName>
            <shortName>MCR II alpha</shortName>
            <ecNumber evidence="1">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme-B sulfoethylthiotransferase alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mrtA</name>
        <name type="synonym">mtrA</name>
        <name type="ordered locus">MJ0083</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="1">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-coenzyme M reductase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64310">
        <property type="entry name" value="C64310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869575.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60391"/>
    <dbReference type="SMR" id="Q60391"/>
    <dbReference type="STRING" id="243232.MJ_0083"/>
    <dbReference type="EnsemblBacteria" id="AAB98063">
        <property type="protein sequence ID" value="AAB98063"/>
        <property type="gene ID" value="MJ_0083"/>
    </dbReference>
    <dbReference type="GeneID" id="1450922"/>
    <dbReference type="KEGG" id="mja:MJ_0083"/>
    <dbReference type="eggNOG" id="arCOG04857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493170_0_0_2"/>
    <dbReference type="InParanoid" id="Q60391"/>
    <dbReference type="OMA" id="VGHQPEY"/>
    <dbReference type="OrthoDB" id="6589at2157"/>
    <dbReference type="PhylomeDB" id="Q60391"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016212">
        <property type="entry name" value="Me_CoM_Rdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009047">
        <property type="entry name" value="Me_CoM_Rdtase_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02249">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000262">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03256">
        <property type="entry name" value="met_CoM_red_alp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147453" description="Methyl-coenzyme M reductase II subunit alpha">
        <location>
            <begin position="1"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </ligand>
        <ligandPart>
            <name>Ni</name>
            <dbReference type="ChEBI" id="CHEBI:28112"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="in chain A" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <note>ligand shared between two alpha subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in chain A" evidence="1">
        <location>
            <begin position="259"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <note>ligand shared between two alpha subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in chain B" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <note>ligand shared between two alpha subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="446"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P11558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="552" mass="61241" checksum="DB66781796B987CE" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2022-05-25" version="17" xmlns="http://uniprot.org/uniprot">
    <accession>P0CW76</accession>
    <name>RELB2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative antitoxin RelB2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">relB2</name>
        <name type="ordered locus">MJ0909.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>IDENTIFICATION</scope>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is RelE2.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496658.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P0CW76"/>
    <dbReference type="SMR" id="P0CW76"/>
    <dbReference type="GeneID" id="27929976"/>
    <dbReference type="OrthoDB" id="130946at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000408468" description="Putative antitoxin RelB2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="61" mass="7093" checksum="3144BE941C838D43" modified="2011-05-03" version="1">MSIVQSYITDEKGNIKGVILDYKTFKKIEELLLDYGLLKAMEEVENEEEIDLETAKKLLEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>P54009</accession>
    <name>RL31_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L31e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl31e</name>
        <name type="ordered locus">MJ0049</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64306">
        <property type="entry name" value="A64306"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54009"/>
    <dbReference type="SMR" id="P54009"/>
    <dbReference type="STRING" id="243232.MJ_0049"/>
    <dbReference type="EnsemblBacteria" id="AAB98030">
        <property type="protein sequence ID" value="AAB98030"/>
        <property type="gene ID" value="MJ_0049"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0049"/>
    <dbReference type="eggNOG" id="arCOG04473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112570_3_2_2"/>
    <dbReference type="InParanoid" id="P54009"/>
    <dbReference type="OMA" id="MEERIYT"/>
    <dbReference type="PhylomeDB" id="P54009"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00410">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_L31e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020052">
        <property type="entry name" value="Ribosomal_L31e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_L31e_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="PTHR10956"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="SSF54575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01144">
        <property type="entry name" value="RIBOSOMAL_L31E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153794" description="50S ribosomal protein L31e">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="87" mass="10205" checksum="74DEEF8900A1BF68" modified="1996-10-01" version="1">MEVLMMEERIYTIPLRDVINKSVRTKRAPRAIKKIKQFLKRHMKAEIVKIDNELNEKIWERGIQKPPARVRVKAVKEGNVVIATLAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>P54013</accession>
    <name>RL21_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L21e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl21e</name>
        <name type="ordered locus">MJ0040</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869531.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54013"/>
    <dbReference type="SMR" id="P54013"/>
    <dbReference type="STRING" id="243232.MJ_0040"/>
    <dbReference type="EnsemblBacteria" id="AAB98021">
        <property type="protein sequence ID" value="AAB98021"/>
        <property type="gene ID" value="MJ_0040"/>
    </dbReference>
    <dbReference type="GeneID" id="1450878"/>
    <dbReference type="KEGG" id="mja:MJ_0040"/>
    <dbReference type="eggNOG" id="arCOG04129">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103610_1_1_2"/>
    <dbReference type="InParanoid" id="P54013"/>
    <dbReference type="OMA" id="KGMPHRR"/>
    <dbReference type="OrthoDB" id="107212at2157"/>
    <dbReference type="PhylomeDB" id="P54013"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_L21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_L21e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_L21e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_L21e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="PTHR20981"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000149689" description="50S ribosomal protein L21e">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="98" mass="11329" checksum="22C9DC88DF7583A3" modified="1998-07-15" version="2">MVQMSEGFRRKTRKKLSKHPRERGLYPITRALREFKEGEYVHIVIDPSVHKGMPHPRFHGRTGIVVGKQGRAFIVKVRDGGKYKQIIAYPQHLRPATA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>P54017</accession>
    <name>RL2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl2</name>
        <name type="ordered locus">MJ0179</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869674.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54017"/>
    <dbReference type="SMR" id="P54017"/>
    <dbReference type="STRING" id="243232.MJ_0179"/>
    <dbReference type="EnsemblBacteria" id="AAB98164">
        <property type="protein sequence ID" value="AAB98164"/>
        <property type="gene ID" value="MJ_0179"/>
    </dbReference>
    <dbReference type="GeneID" id="1451026"/>
    <dbReference type="KEGG" id="mja:MJ_0179"/>
    <dbReference type="eggNOG" id="arCOG04067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_0_3_2"/>
    <dbReference type="InParanoid" id="P54017"/>
    <dbReference type="OMA" id="SCIELRP"/>
    <dbReference type="OrthoDB" id="74812at2157"/>
    <dbReference type="PhylomeDB" id="P54017"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_L2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Rbsml_prot_L2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_L2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_L2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_L2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_L2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_L2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="PTHR13691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129715" description="50S ribosomal protein L2">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="201"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="225"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="242" mass="26103" checksum="3EEDFA3E2C7C0E73" modified="1998-07-15" version="2">MGKRLISQRRGRGSSVYTCPSHKRRGEAKYRRFDELEKKGKVLGKIVDILHDPGRSAPVAKVEYETGEEGLLVVPEGVKVGDIIECGVSAEIKPGNILPLGAIPEGIPVFNIETVPGDGGKLVRAGGCYAHILTHDGERTYVKLPSGHIKALHSMCRATIGVVAGGGRKEKPFVKAGKKYHAMKAKAVKWPRVRGVAMNAVDHPFGGGRHQHTGKPTTVSRKKVPPGRKVGHISARRTGVRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>P54040</accession>
    <name>RL5_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl5</name>
        <name type="ordered locus">MJ0469</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64358">
        <property type="entry name" value="E64358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869969.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54040"/>
    <dbReference type="SMR" id="P54040"/>
    <dbReference type="STRING" id="243232.MJ_0469"/>
    <dbReference type="EnsemblBacteria" id="AAB98458">
        <property type="protein sequence ID" value="AAB98458"/>
        <property type="gene ID" value="MJ_0469"/>
    </dbReference>
    <dbReference type="GeneID" id="8805614"/>
    <dbReference type="KEGG" id="mja:MJ_0469"/>
    <dbReference type="eggNOG" id="arCOG04092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061015_3_0_2"/>
    <dbReference type="InParanoid" id="P54040"/>
    <dbReference type="OMA" id="ERMYAFL"/>
    <dbReference type="OrthoDB" id="97230at2157"/>
    <dbReference type="PhylomeDB" id="P54040"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_L5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_L5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_L5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_L5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_L5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_L5_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="PTHR11994"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125056" description="50S ribosomal protein L5">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <sequence length="190" mass="22169" checksum="BD05366973C3A533" modified="1996-10-01" version="1">MSFEELWQKNPMLKPRIEKVVVNFGVGESGDRLTKGAQVIEELTGQKPIRTRAKQTNPSFGIRKKLPIGLKVTLRGKKAEEFLKNAFEAFQKEGKKLYDYSFDDYGNFSFGIHEHIDFPGQKYDPMIGIFGMDVCVTLERPGFRVKRRKRCRAKIPRRHRLTREEAIEFIEKTFGVKVERVLLEEEEETQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>P54049</accession>
    <name>RL10_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>MjaL10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10</name>
        <name evidence="1" type="synonym">rplP0</name>
        <name type="ordered locus">MJ0509</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="J. Mol. Biol." volume="399" first="214" last="220">
            <title>Structure of a two-domain N-terminal fragment of ribosomal protein L10 from Methanococcus jannaschii reveals a specific piece of the archaeal ribosomal stalk.</title>
            <authorList>
                <person name="Kravchenko O."/>
                <person name="Mitroshin I."/>
                <person name="Nikonov S."/>
                <person name="Piendl W."/>
                <person name="Garber M."/>
            </authorList>
            <dbReference type="PubMed" id="20399793"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2010.04.017"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 10-221</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870010.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3JSY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A/B=10-221"/>
    </dbReference>
    <dbReference type="PDB" id="5D6G">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.30 A"/>
        <property type="chains" value="A=10-221"/>
    </dbReference>
    <dbReference type="PDB" id="5D8H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="B=9-221"/>
    </dbReference>
    <dbReference type="PDB" id="5DAR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="B/E=10-221"/>
    </dbReference>
    <dbReference type="PDBsum" id="3JSY"/>
    <dbReference type="PDBsum" id="5D6G"/>
    <dbReference type="PDBsum" id="5D8H"/>
    <dbReference type="PDBsum" id="5DAR"/>
    <dbReference type="AlphaFoldDB" id="P54049"/>
    <dbReference type="SMR" id="P54049"/>
    <dbReference type="STRING" id="243232.MJ_0509"/>
    <dbReference type="EnsemblBacteria" id="AAB98499">
        <property type="protein sequence ID" value="AAB98499"/>
        <property type="gene ID" value="MJ_0509"/>
    </dbReference>
    <dbReference type="GeneID" id="1451371"/>
    <dbReference type="KEGG" id="mja:MJ_0509"/>
    <dbReference type="eggNOG" id="arCOG04288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_0_0_2"/>
    <dbReference type="InParanoid" id="P54049"/>
    <dbReference type="OMA" id="MAHVAEW"/>
    <dbReference type="OrthoDB" id="73593at2157"/>
    <dbReference type="PhylomeDB" id="P54049"/>
    <dbReference type="EvolutionaryTrace" id="P54049"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_L10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="50S_L10_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_L10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_L10P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="RL10_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="RL10P_insert"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="SSF160369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000154793" description="50S ribosomal protein L10">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="309"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="78"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="140"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="151"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="164"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="169"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="175"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="194"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="203"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="207"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3JSY"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="5D6G"/>
        </source>
    </evidence>
    <sequence length="338" mass="36751" checksum="63A6AFD357E3052D" modified="1998-07-15" version="2">METKVKAHVAPWKIEEVKTLKGLIKSKPVVAIVDMMDVPAPQLQEIRDKIRDKVKLRMSRNTLIIRALKEAAEELNNPKLAELANYVERGAAILVTDMNPFKLYKLLEENKSPAPVRGGQIAPCDIKVEKGSTGMPPGPFLGELKSVGIPAAIEKGKIAIKEDKVVVKKGEVVSPKLAAVLDRLGIKPIKVGLNILAVYEDGIIYTPDVLKVDEEKLLADIQAAYQNAFNLAFNTAYPAKEVLPFLIQKAFINARALSVETAFVTKETAGDILAKAQAQALALASKLPDEALDEDIKAKLSSVEVSAAPAAEEEKEEEKKEEEKKEEDTGAAGLALLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>P81307</accession>
    <name>Y34A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0347.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0347.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869846.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81307"/>
    <dbReference type="STRING" id="243232.MJ_0347.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98344">
        <property type="protein sequence ID" value="AAB98344"/>
        <property type="gene ID" value="MJ_0347.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451204"/>
    <dbReference type="KEGG" id="mja:MJ_0347.1"/>
    <dbReference type="eggNOG" id="arCOG09650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465090_0_0_2"/>
    <dbReference type="OMA" id="NYNTRFY"/>
    <dbReference type="OrthoDB" id="117993at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106818" description="Uncharacterized protein MJ0347.1">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <sequence length="183" mass="21756" checksum="769FCF680E86C895" modified="1998-07-15" version="1">MKTIVIDKSMSVDIEYSINAIDQCNITSLKDLQIQPFDQCIILWNDKIIFRGYCIDARLSQSFGQQQYEYTINSPLWILSQQKTTAKTTFLQIFLKECCNLCNLELDYQLDSNPLIYIEESSYFDALKKCILYTKNYNTRFYVDYEYNSLIFTDKISGQHPKEEKVVGYEFEWDTEIINQVRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>P81334</accession>
    <name>VHUD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>F420-non-reducing hydrogenase vhu iron-sulfur subunit D</fullName>
            <ecNumber>1.12.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vhuD</name>
        <name type="ordered locus">MJ1190.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="J. Mol. Biol." volume="266" first="637" last="641">
            <title>Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion.</title>
            <authorList>
                <person name="Wilting R."/>
                <person name="Schorling S."/>
                <person name="Persson B.C."/>
                <person name="Boeck A."/>
            </authorList>
            <dbReference type="PubMed" id="9102456"/>
            <dbReference type="DOI" id="10.1006/jmbi.1996.0812"/>
        </citation>
        <scope>PROBABLE SELENOCYSTEINE AT SEC-13 AND SEC-64</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The F420-non-reducing hydrogenase vhu is composed of four subunits; VhuA, VhuD, VhuG and VhuU.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MvhD/VhuD family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous termination">
            <sequence resource="EMBL-CDS" id="AAB99196" version="1"/>
        </conflict>
        <text>Truncated C-terminus.</text>
    </comment>
    <dbReference type="EC" id="1.12.99.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99196.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1190.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99196">
        <property type="protein sequence ID" value="AAB99196"/>
        <property type="gene ID" value="MJ_1190.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1190.1"/>
    <dbReference type="eggNOG" id="arCOG02475">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3210745_0_0_2"/>
    <dbReference type="InParanoid" id="P81334"/>
    <dbReference type="OMA" id="XGYGAAD"/>
    <dbReference type="PhylomeDB" id="P81334"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000218278" description="F420-non-reducing hydrogenase vhu iron-sulfur subunit D">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="2">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="134" mass="14710" checksum="45AB45DDF8A7C8C0" modified="2008-02-26" version="3">MDPVIIAFCCYQUGYGAADLAGTSRMQYPATVRIVRLPCTGKFDITYALRAFQKGADAVMVVGUKKGECAYETGNLKAEERVRFAKQLLDELGIGGDRIDMFFMSAAEADKFVSAVNEMTARVEKLGPNPLKAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57591</accession>
    <name>Y127_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative RNase MJ0127</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative toxin MJ0127</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0127</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable toxic component of a putative type VII toxin-antitoxin (TA) system, probably an RNase. Probably neutralized by cognate antitoxin MJ0128. Neutralization may be due to AMPylation by MJ0128.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer, probably forms a complex with cognate antitoxin MJ0128.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Modified by cognate antitoxin MJ0128; probably at least 2 successive AMPylation events occur on Tyr-83.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64315">
        <property type="entry name" value="G64315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869620.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57591"/>
    <dbReference type="SMR" id="Q57591"/>
    <dbReference type="STRING" id="243232.MJ_0127"/>
    <dbReference type="EnsemblBacteria" id="AAB98107">
        <property type="protein sequence ID" value="AAB98107"/>
        <property type="gene ID" value="MJ_0127"/>
    </dbReference>
    <dbReference type="GeneID" id="1450969"/>
    <dbReference type="KEGG" id="mja:MJ_0127"/>
    <dbReference type="eggNOG" id="arCOG05024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142825_3_3_2"/>
    <dbReference type="InParanoid" id="Q57591"/>
    <dbReference type="OMA" id="RHAYHAT"/>
    <dbReference type="OrthoDB" id="112390at2157"/>
    <dbReference type="PhylomeDB" id="Q57591"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000158260" description="Putative RNase MJ0127">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RX(4)HXY motif" evidence="1">
        <location>
            <begin position="76"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-di-AMP-tyrosine" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJR1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="121" mass="14358" checksum="FA3526010E173FEA" modified="1997-11-01" version="1">MPKKDVRAFLYDILENMKDIIDFTNDMTFDEFLKDKKTQKAVIRSLEVIGEAVKNLPEDFINKYPQVPWKGMARLRDKLIHHYFGINYEIIWDIVINKVPNDIKEIEEIIKDIEGEDENSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57605</accession>
    <name>PC2DH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative precorrin-2 dehydrogenase</fullName>
            <ecNumber>1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0140</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the archaeal biosynthesis of heme. Catalyzes the oxiation of precorrin-2 into sirohydroclorin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64317">
        <property type="entry name" value="E64317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869635.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57605"/>
    <dbReference type="SMR" id="Q57605"/>
    <dbReference type="STRING" id="243232.MJ_0140"/>
    <dbReference type="EnsemblBacteria" id="AAB98123">
        <property type="protein sequence ID" value="AAB98123"/>
        <property type="gene ID" value="MJ_0140"/>
    </dbReference>
    <dbReference type="GeneID" id="1450984"/>
    <dbReference type="KEGG" id="mja:MJ_0140"/>
    <dbReference type="eggNOG" id="arCOG01044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_8_2_2"/>
    <dbReference type="InParanoid" id="Q57605"/>
    <dbReference type="OMA" id="THVVVPY"/>
    <dbReference type="OrthoDB" id="94240at2157"/>
    <dbReference type="PhylomeDB" id="Q57605"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="PTHR35330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106716" description="Putative precorrin-2 dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="206" mass="23989" checksum="50C36E48EE4FBC44" modified="1997-11-01" version="1">MLPILLSFEGKKVAVFGCGSVGKRRAKKILKSGGIVDIYSKEFDEEIKKLKESNKNLNLIEIDINQLSDEELKNIIMKYDFIVTAINDEINKRIVKLAKELNKFVNSSTKTEGVNFIIPAYTEVDEVIFSIYTKGKSPLIAKHIRIFVENYLKSTDINMIAYIREFLKETIPKQKDREKILKKIFENEKFREELKKLIEKWENGNH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q57616</accession>
    <name>ACDB1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Acetyl-CoA decarbonylase/synthase complex subunit beta 1</fullName>
            <shortName>ACDS complex subunit beta 1</shortName>
            <ecNumber evidence="3">2.3.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ACDS complex acyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cdhC1</name>
        <name type="ordered locus">MJ0152</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon complex generates CO from CO(2), while the beta subunit (this protein) combines the CO with CoA and a methyl group to form acetyl-CoA. The methyl group, which is incorporated into acetyl-CoA, is transferred to the beta subunit by a corrinoid iron-sulfur protein (the gamma-delta complex).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>acetyl-CoA + Co(I)-[corrinoid Fe-S protein] + H(+) = CO + CoA + methyl-Co(III)-[corrinoid Fe-S protein]</text>
            <dbReference type="Rhea" id="RHEA:45212"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.3.1.169"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="1">Binds 1 [Ni-Fe-S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CdhC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.169" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64319">
        <property type="entry name" value="A64319"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57616"/>
    <dbReference type="SMR" id="Q57616"/>
    <dbReference type="STRING" id="243232.MJ_0152"/>
    <dbReference type="PRIDE" id="Q57616"/>
    <dbReference type="EnsemblBacteria" id="AAB98134">
        <property type="protein sequence ID" value="AAB98134"/>
        <property type="gene ID" value="MJ_0152"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0152"/>
    <dbReference type="eggNOG" id="arCOG02428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_378422_0_0_2"/>
    <dbReference type="InParanoid" id="Q57616"/>
    <dbReference type="OMA" id="GPNQPIP"/>
    <dbReference type="PhylomeDB" id="Q57616"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043884">
        <property type="term" value="F:CO-methylating acetyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01138">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045822">
        <property type="entry name" value="ACS_CODH_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004461">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038571">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023432">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041350">
        <property type="entry name" value="CODH_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42281">
        <property type="entry name" value="PTHR42281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19436">
        <property type="entry name" value="ACS_CODH_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03598">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18537">
        <property type="entry name" value="CODH_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00316">
        <property type="entry name" value="cdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155101" description="Acetyl-CoA decarbonylase/synthase complex subunit beta 1">
        <location>
            <begin position="1"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="569"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="571"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="748" mass="82957" checksum="3BD5E42A4ABA3E8E" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57701</accession>
    <name>Y253_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative hydrogenase maturation protease MJ0253</fullName>
            <ecNumber>3.4.23.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0253</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64331">
        <property type="entry name" value="F64331"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869751.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57701"/>
    <dbReference type="SMR" id="Q57701"/>
    <dbReference type="STRING" id="243232.MJ_0253"/>
    <dbReference type="EnsemblBacteria" id="AAB98240">
        <property type="protein sequence ID" value="AAB98240"/>
        <property type="gene ID" value="MJ_0253"/>
    </dbReference>
    <dbReference type="GeneID" id="1451107"/>
    <dbReference type="KEGG" id="mja:MJ_0253"/>
    <dbReference type="eggNOG" id="arCOG04429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099037_0_2_2"/>
    <dbReference type="InParanoid" id="Q57701"/>
    <dbReference type="OMA" id="GCGNLIF"/>
    <dbReference type="OrthoDB" id="122958at2157"/>
    <dbReference type="PhylomeDB" id="Q57701"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036211">
        <property type="term" value="P:protein modification process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06064">
        <property type="entry name" value="H2MP_F420-Reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0064">Aspartyl protease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000201948" description="Putative hydrogenase maturation protease MJ0253">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="154" mass="16846" checksum="DE03F82A1C91B365" modified="1996-11-01" version="1">MKKKDILIVGCGNLLFGDDGFGCEVVSKLEKMNLPDNVEVIDAGASGAYYLMTLVDEDIKKIIVVDAIDFDLEPGTIKKIDVDELPNIKKYSFDAHNVPLAPFLKDLHNKGIEVVVIGCQGKEFTMPDIKPGLSEEVAKAVDKAIEIILGEIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q57756</accession>
    <name>Y308_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0308</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0308</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64338">
        <property type="entry name" value="E64338"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869806.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57756"/>
    <dbReference type="STRING" id="243232.MJ_0308"/>
    <dbReference type="EnsemblBacteria" id="AAB98294">
        <property type="protein sequence ID" value="AAB98294"/>
        <property type="gene ID" value="MJ_0308"/>
    </dbReference>
    <dbReference type="GeneID" id="1451163"/>
    <dbReference type="KEGG" id="mja:MJ_0308"/>
    <dbReference type="eggNOG" id="arCOG02289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074324_1_0_2"/>
    <dbReference type="InParanoid" id="Q57756"/>
    <dbReference type="OMA" id="RHCEMIQ"/>
    <dbReference type="OrthoDB" id="60124at2157"/>
    <dbReference type="PhylomeDB" id="Q57756"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="PTHR37954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106785" description="Uncharacterized protein MJ0308">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <sequence length="233" mass="26244" checksum="D575639D0E34C079" modified="1996-11-01" version="1">MDVNEIRENAKKLMELMMLDKPFVAVKLAKSKEEIPEGYETLDEEKRHCEMIQMARLERKKLYATVDKHLCKGGAYAMGVFRNPPEPLATGKLYVKLGNFKDEEAAKKTVDAIPKVEEEIYATVYAPLDETDFIPDSIVFIGEPLYALRLVQAILYHKGGRFQADFSGIQSLCADAVAAVYTRKAPNMTLGCNGSRKYAGIKPEEVVVAFPPEKLKDIVEAIEHFRQVWTCGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q57759</accession>
    <name>Y311_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0311</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0311</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64338">
        <property type="entry name" value="H64338"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869809.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57759"/>
    <dbReference type="SMR" id="Q57759"/>
    <dbReference type="STRING" id="243232.MJ_0311"/>
    <dbReference type="EnsemblBacteria" id="AAB98299">
        <property type="protein sequence ID" value="AAB98299"/>
        <property type="gene ID" value="MJ_0311"/>
    </dbReference>
    <dbReference type="GeneID" id="1451166"/>
    <dbReference type="KEGG" id="mja:MJ_0311"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165281_0_0_2"/>
    <dbReference type="OMA" id="YNENTEC"/>
    <dbReference type="OrthoDB" id="137931at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106787" description="Uncharacterized protein MJ0311">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <sequence length="110" mass="12552" checksum="F086C6258FBF8B7B" modified="1996-11-01" version="1">MIQKEILEELKDLDYIHGVLLIKNDGLVEYSSLSEDSNMESLGARLSIILNSISEVIKDIYNEKTECVFIKVKDDGIILIPKDNEILTILFKANNDILHKIIPIIQEIIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q57761</accession>
    <name>SPEE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="1">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Spermidine synthase</fullName>
            <shortName evidence="1">SPDS</shortName>
            <shortName evidence="1">SPDSY</shortName>
            <ecNumber evidence="1">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">speE</name>
        <name type="ordered locus">MJ0313</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>putrescine + S-adenosyl 3-(methylsulfanyl)propylamine = H(+) + S-methyl-5'-thioadenosine + spermidine</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64339">
        <property type="entry name" value="B64339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869811.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57761"/>
    <dbReference type="SMR" id="Q57761"/>
    <dbReference type="STRING" id="243232.MJ_0313"/>
    <dbReference type="EnsemblBacteria" id="AAB98300">
        <property type="protein sequence ID" value="AAB98300"/>
        <property type="gene ID" value="MJ_0313"/>
    </dbReference>
    <dbReference type="GeneID" id="1451168"/>
    <dbReference type="KEGG" id="mja:MJ_0313"/>
    <dbReference type="eggNOG" id="arCOG00050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048199_0_0_2"/>
    <dbReference type="InParanoid" id="Q57761"/>
    <dbReference type="OMA" id="LWPGQSF"/>
    <dbReference type="OrthoDB" id="77175at2157"/>
    <dbReference type="PhylomeDB" id="Q57761"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="PTHR11558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156526" description="Polyamine aminopropyltransferase">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="PABS" evidence="1">
        <location>
            <begin position="10"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="145"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="163"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <sequence length="293" mass="33899" checksum="88E4A8C919E5452A" modified="1996-11-01" version="1">MNQNNDFKCHIWFTEYHNNNVALSVRVKDILYREKSGFQEIEIIDTYDFGKALILDNTFQTTERDEFIYHELISHIPLFTHPNPRNVLVIGGGDGGTVREVVKHKSVETVDFVELDEKVIEACKKYMPKLSCEIDNEKVNLIITDGIKYVAETEKKYDVIIVDCPDPVGPAKGLFEKEFYKNVFKCLNDDGIMVQQSESPLYNLDLIQNICRYLKDAGFKIIMPYTYPMPTYPSGFWSFTLASKKYNPLEVDEARIKEALKDMETKYYDEEVHKGIFLAAPKFLKDAVKKALE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57762</accession>
    <name>Y314_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0314</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0314</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64339">
        <property type="entry name" value="C64339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869812.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57762"/>
    <dbReference type="SMR" id="Q57762"/>
    <dbReference type="EnsemblBacteria" id="AAB98310">
        <property type="protein sequence ID" value="AAB98310"/>
        <property type="gene ID" value="MJ_0314"/>
    </dbReference>
    <dbReference type="GeneID" id="1451169"/>
    <dbReference type="KEGG" id="mja:MJ_0314"/>
    <dbReference type="eggNOG" id="arCOG03156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056064_0_0_2"/>
    <dbReference type="OrthoDB" id="4105at2182"/>
    <dbReference type="PhylomeDB" id="Q57762"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106789" description="Uncharacterized protein MJ0314">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="1">
        <location>
            <begin position="107"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <sequence length="263" mass="30805" checksum="A7520A3BBE0CC5CD" modified="1996-11-01" version="1">MELYKKGYSARTIAKILGCSKSTVCYRLYKLGITPRRGLDLNPQEIIKLYQNGYTTTEIAKIMKCSHETIRRILRNNNIDIRKSSESLIIKNTKKINLNPSESLAYILGVLNGDGSVNKQESNYVIELKVTDKDFIEEFKRNLENIGFKYINEYVRKFENKKDQYVVRVRSKGFYYWYKSLNVDYYMNVIGNNEKLMISWLKGFYDSEGSVVINKKGNYVYKYVSIANTNRNLIDVCCSFLEKLGIEYSVYCEKNNRYKSGYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57788</accession>
    <name>Y342_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0342</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0342</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64342">
        <property type="entry name" value="F64342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869840.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57788"/>
    <dbReference type="SMR" id="Q57788"/>
    <dbReference type="STRING" id="243232.MJ_0342"/>
    <dbReference type="EnsemblBacteria" id="AAB98332">
        <property type="protein sequence ID" value="AAB98332"/>
        <property type="gene ID" value="MJ_0342"/>
    </dbReference>
    <dbReference type="GeneID" id="1451198"/>
    <dbReference type="KEGG" id="mja:MJ_0342"/>
    <dbReference type="eggNOG" id="arCOG09647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2010098_0_0_2"/>
    <dbReference type="OMA" id="LIMEVIH"/>
    <dbReference type="OrthoDB" id="136681at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106812" description="Uncharacterized protein MJ0342">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <sequence length="123" mass="14008" checksum="E2463D19323D5919" modified="1996-11-01" version="1">MRWLTPFGMLFISGTYYGLIFFGLIMEVIHNALISLVLAFFVVFAWDLVLSLIYGLRFVKEGDYIALDWDGQFPDCYGLFASTCLSAVIWTYTDSLLLGLIVPVIIVFLGKQLMRGLYEKIKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q57805</accession>
    <name>Y359_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0359</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0359</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64344">
        <property type="entry name" value="G64344"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57805"/>
    <dbReference type="STRING" id="243232.MJ_0359"/>
    <dbReference type="PRIDE" id="Q57805"/>
    <dbReference type="DNASU" id="1451216"/>
    <dbReference type="EnsemblBacteria" id="AAB98351">
        <property type="protein sequence ID" value="AAB98351"/>
        <property type="gene ID" value="MJ_0359"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0359"/>
    <dbReference type="HOGENOM" id="CLU_1324811_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106830" description="Uncharacterized protein MJ0359">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <sequence length="214" mass="25993" checksum="9A88675453535E8B" modified="1996-11-01" version="1">MMSYVRMTLYHGTDRKSAEKIMESKEILPSQGDNHWLGDGIYFYEEEFHAFKWIWYKEKNRNRLLKNFAIIKAEVICEESRIFDLTKIEHKLLFDMMYKLINTTKLRLDKLRGDMCAEGVVINYMFKNKELGYNKRFDIVRALFPIPVKKYQKIENREKNKKYKERTHRLTFMPEIQVCVKNPSVIKKLEWYDLEKTIDKFLIYTEIYKEDLGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q57818</accession>
    <name>SPT5_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spt5</name>
        <name type="ordered locus">MJ0372</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Proteins" volume="76" first="787" last="793">
            <title>Crystal structure of NusG N-terminal (NGN) domain from Methanocaldococcus jannaschii and its interaction with rpoE''.</title>
            <authorList>
                <person name="Zhou H."/>
                <person name="Liu Q."/>
                <person name="Gao Y."/>
                <person name="Teng M."/>
                <person name="Niu L."/>
            </authorList>
            <dbReference type="PubMed" id="19475703"/>
            <dbReference type="DOI" id="10.1002/prot.22465"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.04 ANGSTROMS) OF 1-83</scope>
        <scope>DOMAIN</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="Nucleic Acids Res." volume="38" first="4040" last="4051">
            <title>Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif.</title>
            <authorList>
                <person name="Hirtreiter A."/>
                <person name="Damsma G.E."/>
                <person name="Cheung A.C."/>
                <person name="Klose D."/>
                <person name="Grohmann D."/>
                <person name="Vojnic E."/>
                <person name="Martin A.C."/>
                <person name="Cramer P."/>
                <person name="Werner F."/>
            </authorList>
            <dbReference type="PubMed" id="20197319"/>
            <dbReference type="DOI" id="10.1093/nar/gkq135"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 1-82</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <scope>MUTAGENESIS OF ALA-4; TYR-42 AND LEU-44</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP). Forms a homodimer in solution.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15739382">
            <id>Q57818</id>
        </interactant>
        <interactant intactId="EBI-15739363">
            <id>Q57839</id>
            <label>spt4</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="domain">
        <text evidence="2 3">Composed of only a NusG N-terminal (NGN) domain and a KOW domain, similar to bacterial NusG. The NGN domain is the effector domain of the complex that mediates the interaction with RNAP and is essential for elongation activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64346">
        <property type="entry name" value="D64346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869871.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3EWG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.04 A"/>
        <property type="chains" value="A=1-83"/>
    </dbReference>
    <dbReference type="PDB" id="3LPE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/C/E/G=1-82"/>
    </dbReference>
    <dbReference type="PDB" id="4ZN1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A=1-147"/>
    </dbReference>
    <dbReference type="PDB" id="4ZN3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-147"/>
    </dbReference>
    <dbReference type="PDBsum" id="3EWG"/>
    <dbReference type="PDBsum" id="3LPE"/>
    <dbReference type="PDBsum" id="4ZN1"/>
    <dbReference type="PDBsum" id="4ZN3"/>
    <dbReference type="AlphaFoldDB" id="Q57818"/>
    <dbReference type="SMR" id="Q57818"/>
    <dbReference type="DIP" id="DIP-46333N"/>
    <dbReference type="IntAct" id="Q57818">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0372"/>
    <dbReference type="EnsemblBacteria" id="AAB98361">
        <property type="protein sequence ID" value="AAB98361"/>
        <property type="gene ID" value="MJ_0372"/>
    </dbReference>
    <dbReference type="GeneID" id="1451229"/>
    <dbReference type="KEGG" id="mja:MJ_0372"/>
    <dbReference type="eggNOG" id="arCOG01920">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113589_0_0_2"/>
    <dbReference type="InParanoid" id="Q57818"/>
    <dbReference type="OMA" id="FSEIEHF"/>
    <dbReference type="OrthoDB" id="91936at2157"/>
    <dbReference type="PhylomeDB" id="Q57818"/>
    <dbReference type="EvolutionaryTrace" id="Q57818"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription, elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_L24/26_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="PTHR11125"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000113978" description="Transcription elongation factor Spt5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="1">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abrogates binding to RNAP. Decreases elongation activity." evidence="3">
        <original>A</original>
        <variation>R</variation>
        <location>
            <position position="4"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abrogates binding to RNAP. Decreases elongation activity." evidence="3">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Can still bind RNAP, but with a decreased affinity. Does not affect elongation activity." evidence="3">
        <original>L</original>
        <variation>A</variation>
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abrogates binding to RNAP. Decreases elongation activity." evidence="3">
        <original>L</original>
        <variation>R</variation>
        <location>
            <position position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="2"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="30"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="50"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="63"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="94"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="114"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="118"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="139"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19475703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="20197319"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3LPE"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4ZN3"/>
        </source>
    </evidence>
    <sequence length="147" mass="16136" checksum="A8D917571BF5F215" modified="1996-11-01" version="1">MIFAVRTMVGQEKNIAGLMASRAEKEQLDVYSILASESLKGYVLVEAETKGDVEELIKGMPRVRGIVPGTIAIEEIEPLLTPKKIIENIEKGDVVEIIAGPFKGERAKVIRVDKHKEEVTLELENAAVPIPITLPVEGVKIVSKHKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q57823</accession>
    <name>CAS1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CRISPR-associated endonuclease Cas1</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cas1</name>
        <name type="ordered locus">MJ0378</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer, forms a heterotetramer with a Cas2 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated endonuclease Cas1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64347">
        <property type="entry name" value="B64347"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869877.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57823"/>
    <dbReference type="SMR" id="Q57823"/>
    <dbReference type="STRING" id="243232.MJ_0378"/>
    <dbReference type="EnsemblBacteria" id="AAB98367">
        <property type="protein sequence ID" value="AAB98367"/>
        <property type="gene ID" value="MJ_0378"/>
    </dbReference>
    <dbReference type="GeneID" id="1451235"/>
    <dbReference type="KEGG" id="mja:MJ_0378"/>
    <dbReference type="eggNOG" id="arCOG01452">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052779_2_0_2"/>
    <dbReference type="InParanoid" id="Q57823"/>
    <dbReference type="OMA" id="AYKLIKH"/>
    <dbReference type="OrthoDB" id="70037at2157"/>
    <dbReference type="PhylomeDB" id="Q57823"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004520">
        <property type="term" value="F:endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09722">
        <property type="entry name" value="Cas1_I-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01470">
        <property type="entry name" value="Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002729">
        <property type="entry name" value="CRISPR-assoc_Cas1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042206">
        <property type="entry name" value="CRISPR-assoc_Cas1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019858">
        <property type="entry name" value="CRISPR-assoc_Cas1_HMARI/TNEAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042211">
        <property type="entry name" value="CRISPR-assoc_Cas1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43219">
        <property type="entry name" value="PTHR43219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01867">
        <property type="entry name" value="Cas_Cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00287">
        <property type="entry name" value="cas1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03641">
        <property type="entry name" value="cas1_HMARI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106841" description="CRISPR-associated endonuclease Cas1">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01470"/>
        </source>
    </evidence>
    <sequence length="322" mass="38147" checksum="6E1833962A77EA3D" modified="1996-11-01" version="1">MRKKSLTLLSDGYLFRKENTIYFENARGKKPLAIEGIYDIYIYGKVSISSQALHYLAQKGIALHFFNHYGYYDGSFYPRESLHSGYLVVNQVEHYLDKDKRLELAKLFIIGGIKNMEWNLLKFKNKTKFSSYIEELNNCNKITEVMNVEGRVRTEYYRLWDETLPDDFKIVKRTRRPPKNEMNALISFLNSRLYPAIITELYNTQLTPTVSYLHEPHERRFSLALDLSEIFKPMIADRLANRLVKQGIIQKKHFRDDLNGVLLNKEGMKVVLEHFNKEMDKTVNHKKLKKNVSKRRLIRLEAYKLVKHLVGQKRYEPLVAWF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q57838</accession>
    <name>DPCKG_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="1">2.7.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="1">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0395</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3'-dephospho-CoA + GTP = CoA + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64349">
        <property type="entry name" value="C64349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869894.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57838"/>
    <dbReference type="STRING" id="243232.MJ_0395"/>
    <dbReference type="EnsemblBacteria" id="AAB98385">
        <property type="protein sequence ID" value="AAB98385"/>
        <property type="gene ID" value="MJ_0395"/>
    </dbReference>
    <dbReference type="GeneID" id="1451252"/>
    <dbReference type="KEGG" id="mja:MJ_0395"/>
    <dbReference type="eggNOG" id="arCOG04076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120795_1_0_2"/>
    <dbReference type="InParanoid" id="Q57838"/>
    <dbReference type="OMA" id="AIYDHKT"/>
    <dbReference type="OrthoDB" id="116732at2157"/>
    <dbReference type="PhylomeDB" id="Q57838"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="PTHR40732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000137608" description="GTP-dependent dephospho-CoA kinase">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <sequence length="158" mass="17968" checksum="56C5423CB778FC8B" modified="1996-11-01" version="1">MLVLPEELREKLKKPFGKVYKTLPDIDGDIVTVGDIVTKTAIENNIIPKLSIFDLKTRRNIPVKINHVFKKVIKVKNPPGCISDEAIESIKYLSTINDRNIALLVDGEEDLLALIVIKYFPIGTYVLYGQPDEGIVVLKINKKLKQEIEEILKQFKKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q57843</accession>
    <name>ADHS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase</fullName>
            <shortName evidence="1">ADH synthase</shortName>
            <shortName evidence="1">ADHS</shortName>
            <shortName evidence="1">ADTH synthase</shortName>
            <ecNumber evidence="1">2.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Transaldolase-like ADHS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroA'</name>
        <name type="ordered locus">MJ0400</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Biochemistry" volume="43" first="7618" last="7627">
            <title>L-aspartate semialdehyde and a 6-deoxy-5-ketohexose 1-phosphate are the precursors to the aromatic amino acids in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="15182204"/>
            <dbReference type="DOI" id="10.1021/bi0495127"/>
        </citation>
        <scope>FUNCTION AS AN ADH SYNTHASE</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="FEMS Microbiol. Lett." volume="281" first="36" last="41">
            <title>MJ0400 from Methanocaldococcus jannaschii exhibits fructose-1,6-bisphosphate aldolase activity.</title>
            <authorList>
                <person name="Samland A.K."/>
                <person name="Wang M."/>
                <person name="Sprenger G.A."/>
            </authorList>
            <dbReference type="PubMed" id="18318840"/>
            <dbReference type="DOI" id="10.1111/j.1574-6968.2008.01079.x"/>
        </citation>
        <scope>FUNCTION AS A FBP ALDOLASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Biochemistry" volume="46" first="10562" last="10571">
            <title>Structure of 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase, a catalyst in the archaeal pathway for the biosynthesis of aromatic amino acids.</title>
            <authorList>
                <person name="Morar M."/>
                <person name="White R.H."/>
                <person name="Ealick S.E."/>
            </authorList>
            <dbReference type="PubMed" id="17713928"/>
            <dbReference type="DOI" id="10.1021/bi700934v"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>SUBUNIT</scope>
        <scope>ACTIVE SITE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 4">Catalyzes a transaldol reaction between 6-deoxy-5-ketofructose 1-phosphate (DKFP) and L-aspartate semialdehyde (ASA) with an elimination of hydroxypyruvaldehyde phosphate to yield 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate (ADH). Plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids. Can also catalyze the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate (GAP) and dihydroxyacetone phosphate (DHAP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + L-aspartate 4-semialdehyde = 2,3-dioxopropyl phosphate + 2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate</text>
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            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="ChEBI" id="CHEBI:58860"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="2.2.1.10"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Inhibited by NaBH4 in the presence of FBP.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="4">430 uM for fructose-1,6-bisphosphate (at pH 7 and 50 degrees Celsius)</KM>
            <Vmax evidence="4">33.0 nmol/min/mg enzyme for the FBP aldolase activity (at pH 7 and 50 degrees Celsius)</Vmax>
        </kinetics>
        <temperatureDependence>
            <text evidence="4">Optimum temperature is not reached at 100 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family. ADHS subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="resolution" value="2.60 A"/>
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        <property type="resolution" value="2.80 A"/>
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    <dbReference type="STRING" id="243232.MJ_0400"/>
    <dbReference type="EnsemblBacteria" id="AAB98389">
        <property type="protein sequence ID" value="AAB98389"/>
        <property type="gene ID" value="MJ_0400"/>
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    <dbReference type="GeneID" id="1451260"/>
    <dbReference type="KEGG" id="mja:MJ_0400"/>
    <dbReference type="eggNOG" id="arCOG04044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_2_0_2"/>
    <dbReference type="InParanoid" id="Q57843"/>
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    <dbReference type="OrthoDB" id="57391at2157"/>
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        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q57843"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0004332">
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    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd00958">
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    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
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    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
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    <dbReference type="Pfam" id="PF01791">
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    </dbReference>
    <dbReference type="SMART" id="SM01133">
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    <dbReference type="TIGRFAMs" id="TIGR01949">
        <property type="entry name" value="AroFGH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0808">Transferase</keyword>
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    <feature type="active site" description="Proton acceptor" evidence="5">
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    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
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            <position position="153"/>
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    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="5">
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    </feature>
    <feature type="binding site" evidence="5">
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            <begin position="33"/>
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        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
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        </ligand>
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        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
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    <evidence type="ECO:0000255" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000269" key="2">
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    <evidence type="ECO:0007829" key="6">
        <source>
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        </source>
    </evidence>
    <sequence length="273" mass="29675" checksum="06DDA2E51E8275E6" modified="1996-11-01" version="1">MELFKDIKNLGKLVRLERIFNRESEKTVIVPMDHGVSNGPIKGLIDIRKTVNDVAEGGANAVLLHKGIVRHGHRGYGKDVGLIIHLSGGTAISPNPLKKVIVTTVEEAIRMGADAVSIHVNVGSDEDWEAYRDLGMIAETCEYWGMPLIAMMYPRGKHIQNERDPELVAHAARLGAELGADIVKTSYTGDIDSFRDVVKGCPAPVVVAGGPKTNTDEEFLQMIKDAMEAGAAGVAVGRNIFQHDDVVGITRAVCKIVHENADVEEALKEIRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q57868</accession>
    <name>Y425_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0425</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0425</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64353">
        <property type="entry name" value="A64353"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57868"/>
    <dbReference type="STRING" id="243232.MJ_0425"/>
    <dbReference type="EnsemblBacteria" id="AAB98412">
        <property type="protein sequence ID" value="AAB98412"/>
        <property type="gene ID" value="MJ_0425"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0425"/>
    <dbReference type="eggNOG" id="arCOG03171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142661_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q57868"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106870" description="Uncharacterized protein MJ0425">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <sequence length="140" mass="16447" checksum="280D4027E1D06ED7" modified="1997-11-01" version="1">MVQVVYPNMADLEIGNVKEVYNKILASLNEYYGKMFENLVFEMLKLKIIDFGQKSVAKWWHKGEEIDVLAYNNNKMIAFEVKWKDLSFKEAKGILRDLERKLDKVDFDGEKECYIIAKSIEGKEKLKALDLMDLEKLIIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57907</accession>
    <name>DPH2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="2">2.5.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Diphthamide biosynthesis protein Dph2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">dph2</name>
        <name type="ordered locus">MJ0483</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + H(+) + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text>Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Unlike the enzymes in the radical SAM superfamily, Dph2 does not form the canonical 5'-deoxyadenosyl radical. Instead, it breaks the C(gamma)-S bond of SAM and generates a 3-amino-3-carboxypropyl radical intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64360">
        <property type="entry name" value="C64360"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57907"/>
    <dbReference type="SMR" id="Q57907"/>
    <dbReference type="STRING" id="243232.MJ_0483"/>
    <dbReference type="PRIDE" id="Q57907"/>
    <dbReference type="EnsemblBacteria" id="AAB98474">
        <property type="protein sequence ID" value="AAB98474"/>
        <property type="gene ID" value="MJ_0483"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0483"/>
    <dbReference type="eggNOG" id="arCOG04112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037146_0_0_2"/>
    <dbReference type="InParanoid" id="Q57907"/>
    <dbReference type="OMA" id="PGQVLGC"/>
    <dbReference type="PhylomeDB" id="Q57907"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="PTHR10762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106891" description="2-(3-amino-3-carboxypropyl)histidine synthase">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O58832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="340" mass="40014" checksum="9BBFA6B67179F392" modified="1996-11-01" version="1">MLFKTIFYQKLQKKVTTLFNLETERVIREIENLNKNNPKVIFQAPEGLKLKVEKEIEKIKQYFKQKNINIEIYLWGNTCFGACDLIDNHVKNLNVDLIIHYGHEKLSYANPEIKTLFIPAYHIFNKDEEEKILNDIKNFIEKHKSGGKKVAIATTIQYKKLLKDFNPSIILGCRGEVKEGDVILFVGTGRFHPLMIAYKYQKEVFIYNPLSKCFDKISEEEINKFIKKRILAISKLLLNKPKKVGVVLSTKKGQCRKRVFDEIIKLLEENDVNYLPILVDNISPDILFYDVDCYIIVACPRIVLDDYILYKKPIYTPEEFKLFLKNSFKYKFDEIKEDDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q57950</accession>
    <name>Y530_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ZPR1-like protein MJ0530</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0530</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64366">
        <property type="entry name" value="B64366"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870034.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57950"/>
    <dbReference type="SMR" id="Q57950"/>
    <dbReference type="STRING" id="243232.MJ_0530"/>
    <dbReference type="EnsemblBacteria" id="AAB98521">
        <property type="protein sequence ID" value="AAB98521"/>
        <property type="gene ID" value="MJ_0530"/>
    </dbReference>
    <dbReference type="GeneID" id="1451395"/>
    <dbReference type="KEGG" id="mja:MJ_0530"/>
    <dbReference type="eggNOG" id="arCOG04265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107446_0_0_2"/>
    <dbReference type="InParanoid" id="Q57950"/>
    <dbReference type="OMA" id="HSAIIGD"/>
    <dbReference type="OrthoDB" id="109705at2157"/>
    <dbReference type="PhylomeDB" id="Q57950"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="Znf_ZPR1-type_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="PTHR10876"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03367">
        <property type="entry name" value="zf-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000119041" description="Uncharacterized ZPR1-like protein MJ0530">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type">
        <location>
            <begin position="9"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="198" mass="22591" checksum="230B073CCA8C3C1A" modified="1996-11-01" version="1">MENVQRLDCPVCGGKGTFVITSHQIDIPYFGPVLETTMICEKCNFRRSDVFPLEVREPKKYILKIESERDLNKRVVRSSSAYIQIPELGVEIKPGPLAEGFVSNVEGVLNRVDNILQTLIRWAETEEQKKKAEELRERIKKLKEGKEEATLILIDPLGHSAIIGEGVEEEILSEEEVEKLKEGIVIMDLDKDKEKEKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57957</accession>
    <name>KORB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2-oxoglutarate synthase subunit KorB</fullName>
            <ecNumber>1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>2-ketoglutarate oxidoreductase beta chain</fullName>
            <shortName>KOR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>2-oxoglutarate-ferredoxin oxidoreductase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">korB</name>
        <name type="ordered locus">MJ0537</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2-oxoglutarate + CoA + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text>Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64367">
        <property type="entry name" value="A64367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870041.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57957"/>
    <dbReference type="SMR" id="Q57957"/>
    <dbReference type="STRING" id="243232.MJ_0537"/>
    <dbReference type="DNASU" id="1451402"/>
    <dbReference type="EnsemblBacteria" id="AAB98531">
        <property type="protein sequence ID" value="AAB98531"/>
        <property type="gene ID" value="MJ_0537"/>
    </dbReference>
    <dbReference type="GeneID" id="1451402"/>
    <dbReference type="KEGG" id="mja:MJ_0537"/>
    <dbReference type="eggNOG" id="arCOG01599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048564_2_0_2"/>
    <dbReference type="InParanoid" id="Q57957"/>
    <dbReference type="OMA" id="SAWIASP"/>
    <dbReference type="OrthoDB" id="43666at2157"/>
    <dbReference type="PhylomeDB" id="Q57957"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000099942" description="2-oxoglutarate synthase subunit KorB">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <sequence length="270" mass="29895" checksum="4F7331CF37216554" modified="1996-11-01" version="1">MHPALKYMRQDRLPHIFCSGCGNGIVMNCFLKAIEELNIKPEDYIAVSGIGCSSRVPGYLYCDSLHTTHGRPIAFATGIKIARPDKHVVVFTGDGDLAAIGGNHFIHGCRRNIDLTVICINNNIYGMTGGQVSPTTPYGKKATTAPYGSIENTMDLCKMAIAAGATYVARWTTAHPIQLVRSIKKGIQKKGFAFIEVVSQCPTYYGRFNISRKPADMIKFLKENSIHLNKAKDMSEEELNGKIVVGEFLDIEKPEFVEELHKLIEKLKSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q57972</accession>
    <name>CBIJ_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt-precorrin-6A reductase</fullName>
            <ecNumber>1.3.1.106</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cbiJ</name>
        <name type="ordered locus">MJ0552</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of the macrocycle of cobalt-precorrin-6A to cobalt-precorrin-6B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Co-precorrin-6B + NAD(+) = Co-precorrin-6A + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:15625"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="1.3.1.106"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 7/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the precorrin-6x reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.106"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64368">
        <property type="entry name" value="H64368"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57972"/>
    <dbReference type="SMR" id="Q57972"/>
    <dbReference type="STRING" id="243232.MJ_0552"/>
    <dbReference type="EnsemblBacteria" id="AAB98544">
        <property type="protein sequence ID" value="AAB98544"/>
        <property type="gene ID" value="MJ_0552"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0552"/>
    <dbReference type="eggNOG" id="arCOG04852">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068627_0_0_2"/>
    <dbReference type="InParanoid" id="Q57972"/>
    <dbReference type="OMA" id="THPFAIN"/>
    <dbReference type="PhylomeDB" id="Q57972"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00228"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016994">
        <property type="term" value="F:precorrin-6A reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003723">
        <property type="entry name" value="Precorrin-6x_reduct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36925">
        <property type="entry name" value="PTHR36925"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02571">
        <property type="entry name" value="CbiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00715">
        <property type="entry name" value="precor6x_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51014">
        <property type="entry name" value="COBK_CBIJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135917" description="Cobalt-precorrin-6A reductase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00356"/>
        </source>
    </evidence>
    <sequence length="249" mass="28177" checksum="7271551E24AB05FE" modified="1996-11-01" version="1">MNLLLMGGTKDSVEIGKKLRDLGDLFILYTSTTDYGGKLGEEFANKVITKPLDKNELKEVIKKYNIDILVDATHPFAINASKNAIEVCKELNIKYVRFERKEEKINHPNIIYVKDFEEAARLAKKANKVFHMAGIKNLKMVVDIVGKDKVIARVLPISVSEALKILPQKQIVAMYGTFSKELNKYLIRDYNCDVIITKDSGESGGFKEKVYGALEAEAKVIVVERPKIDYPVCFDDIDELIKYIANLKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q57993</accession>
    <name>Y573_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0573</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0573</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OsmC/Ohr family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64371">
        <property type="entry name" value="E64371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870077.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57993"/>
    <dbReference type="SMR" id="Q57993"/>
    <dbReference type="STRING" id="243232.MJ_0573"/>
    <dbReference type="EnsemblBacteria" id="AAB98573">
        <property type="protein sequence ID" value="AAB98573"/>
        <property type="gene ID" value="MJ_0573"/>
    </dbReference>
    <dbReference type="GeneID" id="1451438"/>
    <dbReference type="KEGG" id="mja:MJ_0573"/>
    <dbReference type="eggNOG" id="arCOG03686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431650_0_0_2"/>
    <dbReference type="InParanoid" id="Q57993"/>
    <dbReference type="OMA" id="EMAPKMM"/>
    <dbReference type="OrthoDB" id="91920at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="SSF82784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000172734" description="Uncharacterized protein MJ0573">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="189" mass="21198" checksum="47173D93ADBEDF69" modified="1996-11-01" version="1">MNPEMIMEMMNSDIAKEMAPKMMPKMMPLALEKFLQYIPENERKEFIARIVDIIVSKDENKEVSAEYLDMFEALLNVKGLKIHSRGGKGAIKEKISPMDLFLAGLCGCVCIAVGNTLKANNIDAEIKVDGKVEKSFEEGKIKKVIINIYVKVDGDIDKEKLKKLVLEGSKKCLISNSISCEIEKNVILE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58062</accession>
    <name>Y646_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0305 protein MJ0646</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0646</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0305 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64380">
        <property type="entry name" value="F64380"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870151.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58062"/>
    <dbReference type="STRING" id="243232.MJ_0646"/>
    <dbReference type="EnsemblBacteria" id="AAB98640">
        <property type="protein sequence ID" value="AAB98640"/>
        <property type="gene ID" value="MJ_0646"/>
    </dbReference>
    <dbReference type="GeneID" id="1451512"/>
    <dbReference type="KEGG" id="mja:MJ_0646"/>
    <dbReference type="eggNOG" id="arCOG03215">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089549_1_0_2"/>
    <dbReference type="OMA" id="YYCPVKN"/>
    <dbReference type="OrthoDB" id="104709at2157"/>
    <dbReference type="PhylomeDB" id="Q58062"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00763">
        <property type="entry name" value="UPF0305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019215">
        <property type="entry name" value="DUF2115"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09888">
        <property type="entry name" value="DUF2115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004959">
        <property type="entry name" value="UCP004959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141703" description="UPF0305 protein MJ0646">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00763"/>
        </source>
    </evidence>
    <sequence length="164" mass="19895" checksum="8BF99216F5B7A702" modified="1996-11-01" version="1">MKARELFEKLKKELNNFSIYDIMMIRSFIEKDAKYLPPQYKNHYVEAMMKYLIETFNEIRKKSVDEIQDEEIDEEKLNEMLNRIERFRKYYTPDEERFINLSKILCPYLAFIAKKPLHPEYLTFPGNVKIIKKGNNYYCPVKNKQLNEYSLCEFCVCKSIDELK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q58070</accession>
    <name>PYRDB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit</fullName>
            <shortName>DHOD B</shortName>
            <shortName>DHODase B</shortName>
            <shortName>DHOdehase B</shortName>
            <ecNumber>1.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Dihydroorotate oxidase B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Orotate reductase (NADH)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pyrD</name>
        <name type="ordered locus">MJ0654</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dihydroorotate to orotate with NAD(+) as electron acceptor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(S)-dihydroorotate + NAD(+) = H(+) + NADH + orotate</text>
            <dbReference type="Rhea" id="RHEA:13513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.3.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.14"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64381">
        <property type="entry name" value="F64381"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58070"/>
    <dbReference type="SMR" id="Q58070"/>
    <dbReference type="STRING" id="243232.MJ_0654"/>
    <dbReference type="EnsemblBacteria" id="AAB98649">
        <property type="protein sequence ID" value="AAB98649"/>
        <property type="gene ID" value="MJ_0654"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0654"/>
    <dbReference type="eggNOG" id="arCOG00603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042042_0_1_2"/>
    <dbReference type="InParanoid" id="Q58070"/>
    <dbReference type="OMA" id="SCPHAKG"/>
    <dbReference type="PhylomeDB" id="Q58070"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004589">
        <property type="term" value="F:orotate reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148409" description="Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit">
        <location>
            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="195"/>
            <end position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="268"/>
            <end position="269"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="306" mass="32940" checksum="E3532389BA9FC7C3" modified="1996-11-01" version="1">MGECMLKTNICGIEFKNPVFLASGIMGETGSALKRIAKGGAGAVTTKSIGLNPNPGHKNPTIVEVYGGFLNAMGLPNPGVDEYLEEIEKVRDELNRMDVRIIGSIYGKDEEEFAEVAKKMERYVDIIELNISCPHAKGYGATIGQNPDLSYDVCKAVKKAVKIPVFAKLTPNVTDIIEIAQAVVDAGVDGLVAINTVRGMAIDIRAKKPILANKFGGLSGKAIKSIGIKVVWDLYENFDVPIIGVGGIMSGEDAIEYMMAGASAVQIGSGVYYRGYDIFKKVCDEIISFLKEENLTLEEIVGMAHE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58098</accession>
    <name>Y685_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0685</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0685</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0084 and MJ0823.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64385">
        <property type="entry name" value="E64385"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870190.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58098"/>
    <dbReference type="SMR" id="Q58098"/>
    <dbReference type="STRING" id="243232.MJ_0685"/>
    <dbReference type="EnsemblBacteria" id="AAB98680">
        <property type="protein sequence ID" value="AAB98680"/>
        <property type="gene ID" value="MJ_0685"/>
    </dbReference>
    <dbReference type="GeneID" id="1451551"/>
    <dbReference type="KEGG" id="mja:MJ_0685"/>
    <dbReference type="eggNOG" id="arCOG00587">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082962_0_0_2"/>
    <dbReference type="InParanoid" id="Q58098"/>
    <dbReference type="OMA" id="GCYCAVN"/>
    <dbReference type="OrthoDB" id="72034at2157"/>
    <dbReference type="PhylomeDB" id="Q58098"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106988" description="Uncharacterized ATP-binding protein MJ0685">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="253" mass="28585" checksum="A48824E3DC0A8E5D" modified="1996-11-01" version="1">MIIAVSGKGGVGKTAFTTLLIKALSKKTNSILVVDADPDSNLPETLGVEVEKTVGDIREELKKLVERDEIPAGMTKLDYLRSKIFEILVETKYYDLLVMGRPEGSGCYCSVNNWLRQIIDNLAKDYEFVVIDTEAGLEHLSRRTTQNVDVMIVITDASKRGLGTAKRIKKLANELEVKFKDIYVVANKVKPEYEELIDNYAKELGLNLIGKLPYNKEIAEYDLKGIPLWNLPENNEVYKKVEEIAEKIINKKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58102</accession>
    <name>Y690_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0690</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0690</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64386">
        <property type="entry name" value="B64386"/>
    </dbReference>
    <dbReference type="PDB" id="3K32">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-200"/>
    </dbReference>
    <dbReference type="PDBsum" id="3K32"/>
    <dbReference type="AlphaFoldDB" id="Q58102"/>
    <dbReference type="SMR" id="Q58102"/>
    <dbReference type="STRING" id="243232.MJ_0690"/>
    <dbReference type="DNASU" id="1451556"/>
    <dbReference type="EnsemblBacteria" id="AAB98685">
        <property type="protein sequence ID" value="AAB98685"/>
        <property type="gene ID" value="MJ_0690"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0690"/>
    <dbReference type="eggNOG" id="arCOG00037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381418_0_0_2"/>
    <dbReference type="InParanoid" id="Q58102"/>
    <dbReference type="OMA" id="FYDVTLV"/>
    <dbReference type="PhylomeDB" id="Q58102"/>
    <dbReference type="EvolutionaryTrace" id="Q58102"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106991" description="Uncharacterized protein MJ0690">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="4"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="28"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="38"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="56"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
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            <end position="95"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="100"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="151"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="158"/>
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        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="190"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="3K32"/>
        </source>
    </evidence>
    <sequence length="200" mass="22905" checksum="14257CFBF3F59080" modified="1996-11-01" version="1">MKLMDVHVLFSGGKDSSLSAVILKKLGYNPHLITINFGVIPSYKLAEETAKILGFKHKVITLDRKIVEKAADMIIEHKYPGPAIQYVHKTVLEILADEYSILADGTRRDDRVPKLSYSEIQSLEMRKNIQYITPLMGFGYKTLRHLASEFFILEEIKSGTKLSSDYEAEIRHILKERGESPEKYFPEHKQTRVVGLKKEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58147</accession>
    <name>Y737_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0737</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0737</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64392">
        <property type="entry name" value="A64392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870242.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58147"/>
    <dbReference type="SMR" id="Q58147"/>
    <dbReference type="STRING" id="243232.MJ_0737"/>
    <dbReference type="EnsemblBacteria" id="AAB98732">
        <property type="protein sequence ID" value="AAB98732"/>
        <property type="gene ID" value="MJ_0737"/>
    </dbReference>
    <dbReference type="GeneID" id="8512769"/>
    <dbReference type="KEGG" id="mja:MJ_0737"/>
    <dbReference type="eggNOG" id="arCOG05047">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202417_0_0_2"/>
    <dbReference type="OMA" id="DVTCYTP"/>
    <dbReference type="OrthoDB" id="118471at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135074" description="Uncharacterized protein MJ0737">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00241"/>
        </source>
    </evidence>
    <sequence length="61" mass="7051" checksum="EB62A73AF9D15587" modified="1996-11-01" version="1">MPWWKCSNCGYVFEAEKPPERCPNCGEKCTFYDVSCYTPECGFKGYDPKLVARTPNQESKM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58155</accession>
    <name>Y745_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0745</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0745</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the M.jannaschii MJ0150/MJ0739/MJ0745/MJ1460/MJ1642 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64393">
        <property type="entry name" value="A64393"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58155"/>
    <dbReference type="SMR" id="Q58155"/>
    <dbReference type="STRING" id="243232.MJ_0745"/>
    <dbReference type="EnsemblBacteria" id="AAB98745">
        <property type="protein sequence ID" value="AAB98745"/>
        <property type="gene ID" value="MJ_0745"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0745"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1551833_0_0_2"/>
    <dbReference type="InParanoid" id="Q58155"/>
    <dbReference type="OMA" id="ICYFDAG"/>
    <dbReference type="PhylomeDB" id="Q58155"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107012" description="Uncharacterized protein MJ0745">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="181" mass="21150" checksum="9C25AA8EECF08DCE" modified="1996-11-01" version="1">MKNMRVELKTKDMKEFYKIFSESEFIITDDSKLINETVNYLKKKYKNSTTKKKLIPLDLFKAIVLIMMKKIKELDSEITLYDIGYEFGKHLNPKRYSDLKKFFKENNLGTLKVDSRKPLVLKVENCSFCEDLSFEEPICYFDAGLIAGAYECILKKPVVVDEIKCMARGDDACYFKVEVVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58176</accession>
    <name>Y766_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0766</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0766</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0582.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64395">
        <property type="entry name" value="F64395"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58176"/>
    <dbReference type="STRING" id="243232.MJ_0766"/>
    <dbReference type="EnsemblBacteria" id="AAB98773">
        <property type="protein sequence ID" value="AAB98773"/>
        <property type="gene ID" value="MJ_0766"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0766"/>
    <dbReference type="eggNOG" id="arCOG02639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147304_5_0_2"/>
    <dbReference type="OMA" id="SEPACRY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014925">
        <property type="entry name" value="CGGC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08821">
        <property type="entry name" value="CGGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01078">
        <property type="entry name" value="CGGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107023" description="Uncharacterized protein MJ0766">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="66" mass="7235" checksum="A206FD6CB000CCCB" modified="1996-11-01" version="1">MSVITCGGCPGRLGLNQIKQLIGKNGAEVVHFATCMTAFKPKCRYAEKMKEEIEKMGAKVVMSSHF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58190</accession>
    <name>Y780_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0780</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0780</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64397">
        <property type="entry name" value="D64397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870285.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58190"/>
    <dbReference type="SMR" id="Q58190"/>
    <dbReference type="STRING" id="243232.MJ_0780"/>
    <dbReference type="EnsemblBacteria" id="AAB98779">
        <property type="protein sequence ID" value="AAB98779"/>
        <property type="gene ID" value="MJ_0780"/>
    </dbReference>
    <dbReference type="GeneID" id="1451657"/>
    <dbReference type="KEGG" id="mja:MJ_0780"/>
    <dbReference type="eggNOG" id="arCOG01812">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_827975_0_0_2"/>
    <dbReference type="InParanoid" id="Q58190"/>
    <dbReference type="OMA" id="DYQYAGI"/>
    <dbReference type="OrthoDB" id="74259at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107031" description="Uncharacterized protein MJ0780">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="335" mass="38139" checksum="C6028EDBA1455C08" modified="1996-11-01" version="1">MKGIFEKLKRRIDILLYKLGIRPLSIETLKELKESRKEREVLEFYDVYMEPEEFVDIEKYEFILYEGDIVGKTAESLSKIFKGNLFPSRNELRYMGVKDEVAYFKKVVIYMIITFLALLFMGLLDNNLLQGFVNGLIGAGIILVLSLFYPKIRLILFKGEIKLQILFTLIYMISILRAGASLPEVLESISKSREYGVVAFEAKSIIRDVNIGGYNLVEALERAKMRTRIPILKKLYDQMIVGYNKGNLPLLLGKLYEDIVRESMVKLDSSKFMIQNLGNLAFGVGLILPFTGMILSTMIGNQGFSGILSTINLLLLKIGPLLTLIFGIFVKLKIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58276</accession>
    <name>Y866_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HIT-like protein MJ0866</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0866</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64408">
        <property type="entry name" value="B64408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870381.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58276"/>
    <dbReference type="SMR" id="Q58276"/>
    <dbReference type="STRING" id="243232.MJ_0866"/>
    <dbReference type="EnsemblBacteria" id="AAB98871">
        <property type="protein sequence ID" value="AAB98871"/>
        <property type="gene ID" value="MJ_0866"/>
    </dbReference>
    <dbReference type="GeneID" id="1451755"/>
    <dbReference type="KEGG" id="mja:MJ_0866"/>
    <dbReference type="eggNOG" id="arCOG00419">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_3_2_2"/>
    <dbReference type="InParanoid" id="Q58276"/>
    <dbReference type="OMA" id="SDCLFCK"/>
    <dbReference type="OrthoDB" id="93754at2157"/>
    <dbReference type="PhylomeDB" id="Q58276"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23089">
        <property type="entry name" value="PTHR23089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="SSF54197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000109834" description="Uncharacterized HIT-like protein MJ0866">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="HIT" evidence="1">
        <location>
            <begin position="3"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <sequence length="129" mass="14749" checksum="DEC95012CEF4339C" modified="1998-12-15" version="2">MCIFCKIINGEIPAKVVYEDEHVLAFLDINPRNKGHTLVVPKKHYERFDEMPDDELCNFIKGVKKTVEVLKKLGFDGYNIVNNNGRVAGQEVNHVHFHIIPRYEGDGEVVKFGEVKNVDLDEVLKEIKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58297</accession>
    <name>COFC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phospho-L-lactate guanylyltransferase</fullName>
            <shortName>LP guanylyltransferase</shortName>
            <ecNumber evidence="1 2">2.7.7.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cofC</name>
        <name type="ordered locus">MJ0887</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Biochemistry" volume="47" first="3033" last="3037">
            <title>Identification and characterization of the 2-phospho-L-lactate guanylyltransferase involved in coenzyme F420 biosynthesis.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="18260642"/>
            <dbReference type="DOI" id="10.1021/bi702475t"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>ROLE IN F420 BIOSYNTHESIS</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2019" name="ACS Chem. Biol." volume="14" first="2088" last="2094">
            <title>Metabolic pathway rerouting in Paraburkholderia rhizoxinica evolved long-overlooked derivatives of coenzyme F420.</title>
            <authorList>
                <person name="Braga D."/>
                <person name="Last D."/>
                <person name="Hasan M."/>
                <person name="Guo H."/>
                <person name="Leichnitz D."/>
                <person name="Uzum Z."/>
                <person name="Richter I."/>
                <person name="Schalk F."/>
                <person name="Beemelmanns C."/>
                <person name="Hertweck C."/>
                <person name="Lackner G."/>
            </authorList>
            <dbReference type="PubMed" id="31469543"/>
            <dbReference type="DOI" id="10.1021/acschembio.9b00605"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Guanylyltransferase that catalyzes the activation of (2S)-2-phospholactate (2-PL) as (2S)-lactyl-2-diphospho-5'-guanosine, via the condensation of 2-PL with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor (PubMed:18260642, PubMed:31469543). Is able to utilize other purine nucleotides including ATP, dGTP and ITP as cosubstrates in place of GTP but with a lower activity. Does not display lactate kinase activity (PubMed:18260642).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>(2S)-2-phospholactate + GTP + H(+) = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:63424"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59906"/>
            <dbReference type="EC" id="2.7.7.68"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">36 uM for (2S)-2-phospholactate</KM>
            <KM evidence="1">56 uM for GTP</KM>
            <Vmax evidence="1">3.0 umol/min/mg enzyme</Vmax>
        </kinetics>
        <temperatureDependence>
            <text evidence="1">Retains all activity when heated up to 90 degrees Celsius for 10 minutes and retained 38% activity after 10 minutes at 100 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CofC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.68" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64410">
        <property type="entry name" value="G64410"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58297"/>
    <dbReference type="SMR" id="Q58297"/>
    <dbReference type="STRING" id="243232.MJ_0887"/>
    <dbReference type="EnsemblBacteria" id="AAB98891">
        <property type="protein sequence ID" value="AAB98891"/>
        <property type="gene ID" value="MJ_0887"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0887"/>
    <dbReference type="eggNOG" id="arCOG04472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076569_2_0_2"/>
    <dbReference type="InParanoid" id="Q58297"/>
    <dbReference type="OMA" id="FRVDYHG"/>
    <dbReference type="PhylomeDB" id="Q58297"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14615"/>
    <dbReference type="BRENDA" id="2.7.7.105">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.7.7.68">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58297"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043814">
        <property type="term" value="F:phospholactate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02114">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002835">
        <property type="entry name" value="CofC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392">
        <property type="entry name" value="PTHR40392"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01983">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03552">
        <property type="entry name" value="F420_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107091" description="2-phospho-L-lactate guanylyltransferase">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="18260642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="31469543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="18260642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="224" mass="25683" checksum="0565EDD51F313278" modified="1996-11-01" version="1">MNCGIKMKVIIPVSPINSLKTRLSEFLSGEERKNLLLNMLKDIIKALDGLDIVIVSRDEEILDFAKNELKAETIKEKYKGLNNAIKQAFEEIEDKEVIIIPADIPLIKKKHIEDILKLSKNYDLIIAPSRGGGTNLLYLKSKDLIEIKYEGFSFLKHLEEAKKRNLRYYIYDSFLISVDINTPEDLGEIFIHGNDTYTKNYLKSLGIDVEPKHSSAGRFVVKRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58298</accession>
    <name>Y888_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable metallo-hydrolase MJ0888</fullName>
            <ecNumber>3.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0888</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64410">
        <property type="entry name" value="H64410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870402.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58298"/>
    <dbReference type="SMR" id="Q58298"/>
    <dbReference type="STRING" id="243232.MJ_0888"/>
    <dbReference type="EnsemblBacteria" id="AAB98892">
        <property type="protein sequence ID" value="AAB98892"/>
        <property type="gene ID" value="MJ_0888"/>
    </dbReference>
    <dbReference type="GeneID" id="1451777"/>
    <dbReference type="KEGG" id="mja:MJ_0888"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_5_4_2"/>
    <dbReference type="InParanoid" id="Q58298"/>
    <dbReference type="OMA" id="GAWGTNC"/>
    <dbReference type="OrthoDB" id="103066at2157"/>
    <dbReference type="PhylomeDB" id="Q58298"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000107092" description="Probable metallo-hydrolase MJ0888">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="206" mass="23229" checksum="AC2C59FF1E80840E" modified="1996-11-01" version="1">MILKLNGYGYSSNSYLIIGKKNILIDPGTSGTFNILMEELERNGIKDIDLIINTHCHFDHTSADYLIEEYFNCPTIIEDKEVKHLKNGDEVTVSSLFGAKLNPPKEIIPLSEIEEELKSYGLEIIRTPGHTYGSISIIYENSLITGDTIFAYGVGRWDLPTGDVIQLRNSINLLERIANERNIDKLYPGHGEIGDRMAFSYAKLFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58318</accession>
    <name>Y908_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0908</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0908</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunit H family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64413">
        <property type="entry name" value="D64413"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58318"/>
    <dbReference type="SMR" id="Q58318"/>
    <dbReference type="STRING" id="243232.MJ_0908"/>
    <dbReference type="PRIDE" id="Q58318"/>
    <dbReference type="EnsemblBacteria" id="AAB98910">
        <property type="protein sequence ID" value="AAB98910"/>
        <property type="gene ID" value="MJ_0908"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0908"/>
    <dbReference type="eggNOG" id="arCOG03022">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002017_1_0_2"/>
    <dbReference type="InParanoid" id="Q58318"/>
    <dbReference type="OMA" id="WETGQAM"/>
    <dbReference type="PhylomeDB" id="Q58318"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051116">
        <property type="term" value="F:cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016851">
        <property type="term" value="F:magnesium chelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011771">
        <property type="entry name" value="BchH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011953">
        <property type="entry name" value="Cobalto_CobN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02025">
        <property type="entry name" value="BchH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02257">
        <property type="entry name" value="cobalto_cobN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000219887" description="Uncharacterized protein MJ0908">
        <location>
            <begin position="1"/>
            <end position="1232"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="1232" mass="142644" checksum="89722AEF2AE22158" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58342</accession>
    <name>HJA2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable archaeal histone 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0932</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or heterodimer with another histone. Dimers then assemble into higher oligomers, with the DNA wrapped around the protein core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64416">
        <property type="entry name" value="D64416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870446.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58342"/>
    <dbReference type="SMR" id="Q58342"/>
    <dbReference type="STRING" id="243232.MJ_0932"/>
    <dbReference type="EnsemblBacteria" id="AAB98934">
        <property type="protein sequence ID" value="AAB98934"/>
        <property type="gene ID" value="MJ_0932"/>
    </dbReference>
    <dbReference type="GeneID" id="8805033"/>
    <dbReference type="KEGG" id="mja:MJ_0932"/>
    <dbReference type="eggNOG" id="arCOG02144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="InParanoid" id="Q58342"/>
    <dbReference type="OMA" id="MANDLPI"/>
    <dbReference type="PhylomeDB" id="Q58342"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154988" description="Probable archaeal histone 2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P19267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="67" mass="7384" checksum="05DE8B391D9C927C" modified="1997-11-01" version="1">MAELPVAPFERILKKAGAERVSRAAAEYLAEAVEEIALEIAKEAVELAKHAKRKTVKVEDIKLALKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q58367</accession>
    <name>Y957_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0957</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0957</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64419">
        <property type="entry name" value="E64419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870471.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58367"/>
    <dbReference type="STRING" id="243232.MJ_0957"/>
    <dbReference type="EnsemblBacteria" id="AAB98970">
        <property type="protein sequence ID" value="AAB98970"/>
        <property type="gene ID" value="MJ_0957"/>
    </dbReference>
    <dbReference type="GeneID" id="1451855"/>
    <dbReference type="KEGG" id="mja:MJ_0957"/>
    <dbReference type="eggNOG" id="arCOG05060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_803208_0_0_2"/>
    <dbReference type="InParanoid" id="Q58367"/>
    <dbReference type="OMA" id="YWKGWID"/>
    <dbReference type="OrthoDB" id="33147at2157"/>
    <dbReference type="PhylomeDB" id="Q58367"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107121" description="Uncharacterized protein MJ0957">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <sequence length="350" mass="42001" checksum="41680EA9C26AF630" modified="1996-11-01" version="1">MGDDIYIPPKPKPKPKPKPEYKYALWYFKYTNTYEKRFNGTVGDLLNRGFNYAIALEKDEGSGTPESGNLREDGEKDGKEFGEFINSELSGIKYIAQIPYYKRGMLEKLKNASKDKKQMEYYINHIYLVKRTLEYWKGWIDGVIESCDSNLVGFYWNFESPGQVSWGFITDWEIAQLSTYIKQKSNELNRKLEFIWIPYINDIENPDNNDIKRLSKYFDYVFVQPHYYIAWKYWCLWNYEKNVSEDIREYWKYQINRYNGYLTQGITKLIEVLNWIKEIPNGYIEMEVDNKIDEYKYHDLINKACDYIKAREFLTGRDIWQIRAYYFDTNIENVDKVRNGAYGIKGCKNW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58390</accession>
    <name>Y981_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0981</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0981</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64422">
        <property type="entry name" value="E64422"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58390"/>
    <dbReference type="STRING" id="243232.MJ_0981"/>
    <dbReference type="EnsemblBacteria" id="AAB98984">
        <property type="protein sequence ID" value="AAB98984"/>
        <property type="gene ID" value="MJ_0981"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0981"/>
    <dbReference type="eggNOG" id="arCOG02680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110112_1_0_2"/>
    <dbReference type="InParanoid" id="Q58390"/>
    <dbReference type="OMA" id="KHERSFK"/>
    <dbReference type="PhylomeDB" id="Q58390"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012041">
        <property type="entry name" value="Znf_CPxCG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42195">
        <property type="entry name" value="PTHR42195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19769">
        <property type="entry name" value="CPxCG_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015877">
        <property type="entry name" value="UCP015877"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107129" description="Uncharacterized protein MJ0981">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <sequence length="204" mass="23595" checksum="6AB7CA2A26421FB6" modified="1996-11-01" version="1">MEMTEKIYLKCENCGFEEQEVLKKKIYNKSAYYLVRCPNCGSVREIVDKVKLSQAKLIISRYDISESKVINIPEDETYKVGDTIEIDGEKIEITKIETPESVKSALGEDIKVIWGKSLSIPKKLGISINDRSKTYGIYIYVPNDFEFEVEKVYRINDGFFRLKKIKTEKGTAKKAKAKDIKRLYGDVTRPVRNYVDLSEFYKGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58397</accession>
    <name>Y990_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative sulfur carrier protein MJ0990</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0990</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfur carrier protein TusA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64423">
        <property type="entry name" value="F64423"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58397"/>
    <dbReference type="SMR" id="Q58397"/>
    <dbReference type="STRING" id="243232.MJ_0990"/>
    <dbReference type="EnsemblBacteria" id="AAB98992">
        <property type="protein sequence ID" value="AAB98992"/>
        <property type="gene ID" value="MJ_0990"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0990"/>
    <dbReference type="eggNOG" id="arCOG02062">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165255_0_1_2"/>
    <dbReference type="InParanoid" id="Q58397"/>
    <dbReference type="OMA" id="VVGDYKP"/>
    <dbReference type="PhylomeDB" id="Q58397"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159080" description="Putative sulfur carrier protein MJ0990">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0A890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="75" mass="8465" checksum="CF7009364C388539" modified="1996-11-01" version="1">MVIAMKKLDVTGDICPVPVLKTKKALEELNEGEELEVVGDYKPALENIKRFAENNGYTVVLAEETESRFRIVIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q58398</accession>
    <name>Y991_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0991</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0991</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64423">
        <property type="entry name" value="G64423"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58398"/>
    <dbReference type="SMR" id="Q58398"/>
    <dbReference type="STRING" id="243232.MJ_0991"/>
    <dbReference type="EnsemblBacteria" id="AAB98996">
        <property type="protein sequence ID" value="AAB98996"/>
        <property type="gene ID" value="MJ_0991"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0991"/>
    <dbReference type="eggNOG" id="arCOG09630">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150439_0_0_2"/>
    <dbReference type="OMA" id="DYDGIDD"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107134" description="Uncharacterized protein MJ0991">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <sequence length="144" mass="17271" checksum="00F1CFCD0007A09E" modified="1996-11-01" version="1">MVIIMPTKTITLKVPSNISKKKIEEAIKKLELEEKYKKTENFKLFVKDEDLKMKIYKIAEFVEDYLKKKYSDEEFEIVLDYDGIDDKVVVEIVFKKKLDKRELKDIKVIIRKLKEIIFDAWRKVDEKYPDMRGFLIVTSDLEVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58405</accession>
    <name>THS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Thermosome subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Chaperonin subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ths</name>
        <name type="ordered locus">MJ0999</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms an oligomeric complex of eight-membered rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64424">
        <property type="entry name" value="F64424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870512.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58405"/>
    <dbReference type="SMR" id="Q58405"/>
    <dbReference type="STRING" id="243232.MJ_0999"/>
    <dbReference type="EnsemblBacteria" id="AAB99002">
        <property type="protein sequence ID" value="AAB99002"/>
        <property type="gene ID" value="MJ_0999"/>
    </dbReference>
    <dbReference type="GeneID" id="1451896"/>
    <dbReference type="KEGG" id="mja:MJ_0999"/>
    <dbReference type="eggNOG" id="arCOG01257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_2"/>
    <dbReference type="InParanoid" id="Q58405"/>
    <dbReference type="OMA" id="HPAANMI"/>
    <dbReference type="OrthoDB" id="11742at2157"/>
    <dbReference type="PhylomeDB" id="Q58405"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="PTHR11353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="SSF48592"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="SSF52029"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="SSF54849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000128390" description="Thermosome subunit">
        <location>
            <begin position="1"/>
            <end position="542"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="542" mass="58772" checksum="D505F08531D4668D" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q58418</accession>
    <name>PSTB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphate import ATP-binding protein PstB</fullName>
            <ecNumber evidence="1">7.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ABC phosphate transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphate-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pstB</name>
        <name type="ordered locus">MJ1012</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex PstSACB involved in phosphate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:24440"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (PstB), two transmembrane proteins (PstC and PstA) and a solute-binding protein (PstS).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Phosphate importer (TC 3.A.1.7) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64426">
        <property type="entry name" value="C64426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870525.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58418"/>
    <dbReference type="SMR" id="Q58418"/>
    <dbReference type="STRING" id="243232.MJ_1012"/>
    <dbReference type="EnsemblBacteria" id="AAB99016">
        <property type="protein sequence ID" value="AAB99016"/>
        <property type="gene ID" value="MJ_1012"/>
    </dbReference>
    <dbReference type="GeneID" id="1451909"/>
    <dbReference type="KEGG" id="mja:MJ_1012"/>
    <dbReference type="eggNOG" id="arCOG00231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q58418"/>
    <dbReference type="OMA" id="TIDICRV"/>
    <dbReference type="OrthoDB" id="78752at2157"/>
    <dbReference type="PhylomeDB" id="Q58418"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015415">
        <property type="term" value="F:ATPase-coupled phosphate ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="Phosp_transpt1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="PTHR43423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51238">
        <property type="entry name" value="PSTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092946" description="Phosphate import ATP-binding protein PstB">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="6"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01702"/>
        </source>
    </evidence>
    <sequence length="252" mass="28688" checksum="CA6F160C5BFEE65B" modified="1996-11-01" version="1">MTKVKMETKNLNLWYGEKQALFDINLPIYENKITALIGPSGCGKSTFLRCLNRLNDLIPNVRIEGEVLLDGKNIYDKDVDVYELRKRVGMVFQKPNPFAMSIYDNVAFGPRIHGIKDKKELDKIVEWALKKAALWDEVKDELHKNALSLSGGQQQRLCIARAIAVKPEVLLMDEPTSALDPISTLKIEELMVELAKDYTIVVVTHNMQQASRVSDYTAFFLMGKLIEFGETEQIFLNPQKKETDDYISGRFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58427</accession>
    <name>Y1021_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1021</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1021</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64427">
        <property type="entry name" value="D64427"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870534.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58427"/>
    <dbReference type="SMR" id="Q58427"/>
    <dbReference type="STRING" id="243232.MJ_1021"/>
    <dbReference type="EnsemblBacteria" id="AAB99025">
        <property type="protein sequence ID" value="AAB99025"/>
        <property type="gene ID" value="MJ_1021"/>
    </dbReference>
    <dbReference type="GeneID" id="1451918"/>
    <dbReference type="KEGG" id="mja:MJ_1021"/>
    <dbReference type="eggNOG" id="arCOG05064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631690_0_0_2"/>
    <dbReference type="OMA" id="MYNEICI"/>
    <dbReference type="OrthoDB" id="88501at2157"/>
    <dbReference type="PhylomeDB" id="Q58427"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107145" description="Uncharacterized protein MJ1021">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <sequence length="163" mass="19561" checksum="3D92438275C4330E" modified="1996-11-01" version="1">MEIERVAELILLKDKNFKEKERLRDLLREYIKTKDEISYLENILEDFENLDVNLKHLKRDADIIKSILPRLSKFTNIPVFMKIVKMLEAVEKIDTEDLESVRWNINKEIEELNDKLKTLENELRVIIINEALSKIGTSNLEEFSKYLENLRYEEKNQKEEAYN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58458</accession>
    <name>Y1058_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1058</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1058</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NodU/CmcH family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64432">
        <property type="entry name" value="A64432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870571.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58458"/>
    <dbReference type="SMR" id="Q58458"/>
    <dbReference type="STRING" id="243232.MJ_1058"/>
    <dbReference type="EnsemblBacteria" id="AAB99062">
        <property type="protein sequence ID" value="AAB99062"/>
        <property type="gene ID" value="MJ_1058"/>
    </dbReference>
    <dbReference type="GeneID" id="1451955"/>
    <dbReference type="KEGG" id="mja:MJ_1058"/>
    <dbReference type="eggNOG" id="arCOG01188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014411_2_0_2"/>
    <dbReference type="InParanoid" id="Q58458"/>
    <dbReference type="OMA" id="YDASKVM"/>
    <dbReference type="OrthoDB" id="34157at2157"/>
    <dbReference type="PhylomeDB" id="Q58458"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031730">
        <property type="entry name" value="Carbam_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038152">
        <property type="entry name" value="Carbam_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003696">
        <property type="entry name" value="Carbtransf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16861">
        <property type="entry name" value="Carbam_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02543">
        <property type="entry name" value="Carbam_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000207858" description="Uncharacterized protein MJ1058">
        <location>
            <begin position="1"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="609" mass="71733" checksum="C91899CF01CB84EA" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58491</accession>
    <name>CBIM_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative cobalt transport protein CbiM</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Energy-coupling factor transporter probable substrate-capture protein CbiM</fullName>
            <shortName evidence="1">ECF transporter S component CbiM</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiM</name>
        <name type="ordered locus">MJ1091</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiM family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64436">
        <property type="entry name" value="B64436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870603.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58491"/>
    <dbReference type="SMR" id="Q58491"/>
    <dbReference type="STRING" id="243232.MJ_1091"/>
    <dbReference type="EnsemblBacteria" id="AAB99092">
        <property type="protein sequence ID" value="AAB99092"/>
        <property type="gene ID" value="MJ_1091"/>
    </dbReference>
    <dbReference type="GeneID" id="1451987"/>
    <dbReference type="KEGG" id="mja:MJ_1091"/>
    <dbReference type="eggNOG" id="arCOG02248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052508_3_0_2"/>
    <dbReference type="InParanoid" id="Q58491"/>
    <dbReference type="OMA" id="ANVFSMG"/>
    <dbReference type="OrthoDB" id="94360at2157"/>
    <dbReference type="PhylomeDB" id="Q58491"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01462">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018024">
        <property type="entry name" value="CbiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43627">
        <property type="entry name" value="PTHR43627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00123">
        <property type="entry name" value="cbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0171">Cobalt transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000089374" description="Putative cobalt transport protein CbiM">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01462"/>
        </source>
    </evidence>
    <sequence length="233" mass="24914" checksum="FDC194B58AA2CF9B" modified="1996-11-01" version="1">MHIMEGYLPPMWCAVWWVLSGIVIAYGIVKLKKLLEESPEMKPLVAISGAYMFILSSLKMPSVTGSCSHPCGNGLGAVLFGVPITAVLAAIVLLFQALFLAHGGLTTLGANDFSMGIVGPAAAVIVYRLCMKAGLSSTVGIFFAALFGDWLTYVTTAVQLALAFPIPSFTAAFTKFIVIYAYTQVPLAIAEGILTVIIWDYIKKLRPDLLLKLGVVPEEELKPYLTPSPAGGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58501</accession>
    <name>GLMU_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Bifunctional protein GlmU</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName>UDP-N-acetylglucosamine pyrophosphorylase</fullName>
                <ecNumber>2.7.7.23</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>N-acetylglucosamine-1-phosphate uridyltransferase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>Glucosamine-1-phosphate N-acetyltransferase</fullName>
                <ecNumber>2.3.1.157</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name type="primary">glmU</name>
        <name type="ordered locus">MJ1101</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Biochemistry" volume="45" first="12366" last="12379">
            <title>Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="White R.H."/>
                <person name="Xu H."/>
            </authorList>
            <dbReference type="PubMed" id="17014089"/>
            <dbReference type="DOI" id="10.1021/bi061018a"/>
        </citation>
        <scope>FUNCTION AS AN URIDYLYLTRANSFERASE</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Bacteriol." volume="190" first="2987" last="2996">
            <title>Acetamido sugar biosynthesis in the Euryarchaea.</title>
            <authorList>
                <person name="Namboori S.C."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="18263721"/>
            <dbReference type="DOI" id="10.1128/JB.01970-07"/>
        </citation>
        <scope>FUNCTION AS A PYROPHOSPHORYLASE AND ACETYLTRANSFERASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>900</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetyl-glucosamine (UDP-GlcNAc). Responsible for the acetylation of GlcN-1-P to GlcNAc-1-P, and for the uridyl transfer from UTP to GlcNAc-1-P, to produce UDP-GlcNAc and pyrophosphate. Also catalyzes the reverse reaction, i.e. the cleavage of UDP-GlcNAc with pyrophosphate to form UTP and GlcNAc-1-P. To a lesser extent, is also able to use dUTP or dTTP as the nucleotide substrate, but not CTP, ATP or GTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="3">Optimum pH is 7.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 50-60 degrees Celsius. Activity is 22% lower at 60 degrees Celsius and 60% lower at 100 degrees. Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text>Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.23"/>
    <dbReference type="EC" id="2.3.1.157"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64437">
        <property type="entry name" value="D64437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870613.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58501"/>
    <dbReference type="SMR" id="Q58501"/>
    <dbReference type="STRING" id="243232.MJ_1101"/>
    <dbReference type="EnsemblBacteria" id="AAB99104">
        <property type="protein sequence ID" value="AAB99104"/>
        <property type="gene ID" value="MJ_1101"/>
    </dbReference>
    <dbReference type="GeneID" id="1451998"/>
    <dbReference type="KEGG" id="mja:MJ_1101"/>
    <dbReference type="eggNOG" id="arCOG00666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_0_1_2"/>
    <dbReference type="InParanoid" id="Q58501"/>
    <dbReference type="OMA" id="TAIVEHK"/>
    <dbReference type="OrthoDB" id="61185at2157"/>
    <dbReference type="PhylomeDB" id="Q58501"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00533"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000068761" description="Bifunctional protein GlmU">
        <location>
            <begin position="1"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Pyrophosphorylase">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Linker">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-acetyltransferase">
        <location>
            <begin position="223"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="6"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>N-acetyl-alpha-D-glucosamine 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>N-acetyl-alpha-D-glucosamine 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>N-acetyl-alpha-D-glucosamine 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>N-acetyl-alpha-D-glucosamine 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="382"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17014089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18263721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="408" mass="46056" checksum="03280F372CCD299A" modified="1996-11-01" version="1">MDAIILCAGKGERLRPLTENRPKPMIPIAGKPILQHIIEKVEDLVDNIYLIVKYKKEKIVDYFKNHPKIKFLEQGEIDGTGQAVLTAKDYVDDEFLVINGDIIFEDDLEEFLKYKYAVAVKEVKNPENFGVVVLDDENNIIELQEKPENPKSNLINAGIYKFDKKIFELIEKTKISERGERELTDAIKHLIKEEKVKGIKLNGYWNDVGRPWDILEANKYLLDKINTDIKGKIEENVVIKGEVIIEEGAIVKANSVIEGPAIIKKGAVVGPLAYIRPYTVLMENTFVGNSSEVKASIIMKNTKIPHLSYVGDSIIGENCNFGCNTITANLRFDDKPVKVNIKSKRVESVRKLGVIMGDNVKTGIQVSFMPGVKVGSNCWIGASCLIDRDIESNTFVYKRDELIFRKLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58521</accession>
    <name>VAPC3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Ribonuclease VapC3</fullName>
            <shortName>RNase VapC3</shortName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative toxin VapC3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">vapC3</name>
        <name type="ordered locus">MJ1121</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB3.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64439">
        <property type="entry name" value="H64439"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58521"/>
    <dbReference type="SMR" id="Q58521"/>
    <dbReference type="STRING" id="243232.MJ_1121"/>
    <dbReference type="EnsemblBacteria" id="AAB99123">
        <property type="protein sequence ID" value="AAB99123"/>
        <property type="gene ID" value="MJ_1121"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1121"/>
    <dbReference type="eggNOG" id="arCOG02219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118482_3_5_2"/>
    <dbReference type="InParanoid" id="Q58521"/>
    <dbReference type="OMA" id="NEMIMID"/>
    <dbReference type="PhylomeDB" id="Q58521"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107176" description="Ribonuclease VapC3">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="94" mass="10719" checksum="BBA60946D299EFB4" modified="1996-11-01" version="1">MDAVIDTSVIIEIFRGNKDTLYQICDYNCKITSITVFELYCGNLKENEMIMIDSLPKLNFDDKSSKIAGNIFKKLKKEGKIPSVKDLLIASIFY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58556</accession>
    <name>Y1156_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Cell division cycle protein 48 homolog MJ1156</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1156</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64444">
        <property type="entry name" value="C64444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870669.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58556"/>
    <dbReference type="SMR" id="Q58556"/>
    <dbReference type="STRING" id="243232.MJ_1156"/>
    <dbReference type="PRIDE" id="Q58556"/>
    <dbReference type="EnsemblBacteria" id="AAB99153">
        <property type="protein sequence ID" value="AAB99153"/>
        <property type="gene ID" value="MJ_1156"/>
    </dbReference>
    <dbReference type="GeneID" id="1452054"/>
    <dbReference type="KEGG" id="mja:MJ_1156"/>
    <dbReference type="eggNOG" id="arCOG01308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_2_2"/>
    <dbReference type="InParanoid" id="Q58556"/>
    <dbReference type="OMA" id="HACHDIK"/>
    <dbReference type="OrthoDB" id="2808at2157"/>
    <dbReference type="PhylomeDB" id="Q58556"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Vps4_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="SSF54585"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000084591" description="Cell division cycle protein 48 homolog MJ1156">
        <location>
            <begin position="1"/>
            <end position="903"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="220"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="493"/>
            <end position="500"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="903" mass="100403" checksum="99F2ABEE2D544DC2" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58579</accession>
    <name>RIBL_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FMN adenylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Flavin adenine dinucleotide synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ribL</name>
        <name type="ordered locus">MJ1179</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="Biochemistry" volume="49" first="8748" last="8755">
            <title>Archaeal RibL: a new FAD synthetase that is air sensitive.</title>
            <authorList>
                <person name="Mashhadi Z."/>
                <person name="Xu H."/>
                <person name="Grochowski L.L."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="20822113"/>
            <dbReference type="DOI" id="10.1021/bi100817q"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>GENE NAME</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF CYS-126 AND CYS-143</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transfer of the AMP portion of ATP to flavin mononucleotide (FMN) to produce flavin adenine dinucleotide (FAD) coenzyme. To a lesser extent, is also able to utilize other nucleotides such as CTP and GTP as substrates, producing the modified coenzymes, flavin cytosine dinucleotide (FCD) and flavin guanine dinucleotide (FGD), respectively. Does not catalyze the reverse reaction to produce FMN and ATP from FAD and PPi. Does not function as a glycerol-3-phosphate cytidylyltransferase, as previously annotated in the complete genome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + FMN + H(+) = diphosphate + FAD</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="2">Divalent metal cations. The best activity is observed with Co(2+), where the activity is 4 and 2.5 times greater than that with Mg(2+) and Mn(2+), respectively.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Is inhibited by the product PPi.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">25 uM for ATP</KM>
            <KM evidence="2">63 uM for FMN</KM>
            <KM evidence="2">480 uM for CTP</KM>
            <Vmax evidence="2">14.0 nmol/min/mg enzyme with ATP and FMN as substrates</Vmax>
            <Vmax evidence="2">10.0 nmol/min/mg enzyme with CTP and FMN as substrates</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Alkylation of both Cys-126 and Cys-143 results in complete loss of enzymatic activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal FAD synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64447">
        <property type="entry name" value="B64447"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870692.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58579"/>
    <dbReference type="SMR" id="Q58579"/>
    <dbReference type="STRING" id="243232.MJ_1179"/>
    <dbReference type="EnsemblBacteria" id="AAB99182">
        <property type="protein sequence ID" value="AAB99182"/>
        <property type="gene ID" value="MJ_1179"/>
    </dbReference>
    <dbReference type="GeneID" id="1452077"/>
    <dbReference type="KEGG" id="mja:MJ_1179"/>
    <dbReference type="eggNOG" id="arCOG01222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034585_2_1_2"/>
    <dbReference type="InParanoid" id="Q58579"/>
    <dbReference type="OMA" id="FAKKHAD"/>
    <dbReference type="OrthoDB" id="79845at2157"/>
    <dbReference type="PhylomeDB" id="Q58579"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-16487"/>
    <dbReference type="BRENDA" id="2.7.7.2">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58579"/>
    <dbReference type="UniPathway" id="UPA00277">
        <property type="reaction ID" value="UER00407"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002135">
        <property type="term" value="F:CTP binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046444">
        <property type="term" value="P:FMN metabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02115">
        <property type="entry name" value="FAD_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024902">
        <property type="entry name" value="FAD_synth_RibL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107202" description="FAD synthase">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="2-fold increase in activity with Mg(2+) but loss of activity with Co(2+) as cofactor." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="126"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Nearly no change in activity with Mg(2+) but loss of activity with Co(2+) as cofactor." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="20822113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="149" mass="17288" checksum="55FD11671ED94594" modified="1996-11-01" version="1">MKKRVVTAGTFDILHPGHYEILKFAKSLGDELIVIVARDETVKKLKGRKPIIPEEQRREMVEALKPVDKAILGSLKNKLEPILELKPDIIVLGPDQTTFDEETLKKELAKYNLYPEIVRFRGYKKCPFHSSFDIVKEIIRRFCNKEIKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58587</accession>
    <name>Y1188_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1188</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64448">
        <property type="entry name" value="B64448"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58587"/>
    <dbReference type="SMR" id="Q58587"/>
    <dbReference type="STRING" id="243232.MJ_1188"/>
    <dbReference type="EnsemblBacteria" id="AAB99192">
        <property type="protein sequence ID" value="AAB99192"/>
        <property type="gene ID" value="MJ_1188"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1188"/>
    <dbReference type="eggNOG" id="arCOG01897">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_7_0_2"/>
    <dbReference type="InParanoid" id="Q58587"/>
    <dbReference type="OMA" id="SLFFWEY"/>
    <dbReference type="PhylomeDB" id="Q58587"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107209" description="Uncharacterized protein MJ1188">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <sequence length="270" mass="32467" checksum="74DBB2C154B943A7" modified="1996-11-01" version="1">MMKIGVSTLFFWEYPMVEIFDIFRDIGIKCMEFFPENPDFWDNRFDLDYIADLRKEFLKFDVALHNPHIELNPSSLNPYVREAVIKETLWSIELAKFYRCKLITIHPGKRPTNRSPTDEEYEAFFKYLDRTLEVAINKNITICVENMPERINRIGWSPEEMEWILKRYDELLYMTLDFAHAKEYMEEFLESVIDYIKHTHISGVVNRKDHFPLRKSEIDFSPYIKALLDYGYNGMFNLELDDRRLEKNPVTKEEKIEEVIKDIEFLESII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58589</accession>
    <name>Y1189_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1189</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1189</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0233.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64448">
        <property type="entry name" value="D64448"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870702.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58589"/>
    <dbReference type="EnsemblBacteria" id="AAB99198">
        <property type="protein sequence ID" value="AAB99198"/>
        <property type="gene ID" value="MJ_1189"/>
    </dbReference>
    <dbReference type="GeneID" id="1452087"/>
    <dbReference type="KEGG" id="mja:MJ_1189"/>
    <dbReference type="eggNOG" id="arCOG02880">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083802_0_0_2"/>
    <dbReference type="OMA" id="FELPEWE"/>
    <dbReference type="OrthoDB" id="119187at2157"/>
    <dbReference type="PhylomeDB" id="Q58589"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107210" description="Uncharacterized protein MJ1189">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="283" mass="31673" checksum="90D0EA63A28BE128" modified="1996-11-01" version="1">MKKLEYYLKDAFYYVLSDVKKGIVGGLLSSTSGAIGAIFGIILSILLIHNINPNDVVGLDNNILLTSLIVASFGFLIALIIGFILDGYYVRVMKTTVENYDVLPDWDDIAELLKRGFLYWIGNIILSIIFMIVPILFIIFGVFLIFLPLVGIVFIGIGFLLLFVSTIALLIYEGLAEVNYSVKGFSGFFEFKEIFRMINLNYIILLIIVGVIVIVINFVVQLPFILLKIFAISPARYSTFSSSETIVDVISAVISAFVGFYTAVFAKRAIALYYKDRVEELKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58596</accession>
    <name>Y1196_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative amino-acid transporter MJ1196</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1196</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid-polyamine-organocation (APC) superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64449">
        <property type="entry name" value="C64449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870708.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58596"/>
    <dbReference type="SMR" id="Q58596"/>
    <dbReference type="STRING" id="243232.MJ_1196"/>
    <dbReference type="EnsemblBacteria" id="AAB99200">
        <property type="protein sequence ID" value="AAB99200"/>
        <property type="gene ID" value="MJ_1196"/>
    </dbReference>
    <dbReference type="GeneID" id="1452091"/>
    <dbReference type="KEGG" id="mja:MJ_1196"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_743174_0_0_2"/>
    <dbReference type="InParanoid" id="Q58596"/>
    <dbReference type="OMA" id="FAYDGWL"/>
    <dbReference type="OrthoDB" id="15038at2157"/>
    <dbReference type="PhylomeDB" id="Q58596"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015655">
        <property type="term" value="F:alanine:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001463">
        <property type="entry name" value="Na/Ala_symport"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00175">
        <property type="entry name" value="NAALASMPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0029">Amino-acid transport</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000054254" description="Putative amino-acid transporter MJ1196">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="346"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="366" mass="40470" checksum="450E9B5DC8EF4C80" modified="1996-11-01" version="1">MGHLTLKDAVFLTITSIVGGGIFVLSPLTYLLFGKSIIWGWALLIFVSLIMASPFAYASTKISESGGVYKFVMKILGREIGVFSAYILWLSGVFALSGVVSFFEIVFNTKFNVSYVGLCLIVILTALILGGLRIVGNFVRIFGILTITIILYIVFSNGIKIDSIGEFNLKNAILTIYFGLWTATGWEGITMPLSAFKNQKAIAYGLLVGTFIIGVLYLLFSLTIVSLNVKTNNLDEILKILIGDNLFLLAGMLLIISSCAFSVLFTLSYMPYGMGKDRIFPKAFIKLRKEIPYYGVILNTLLVIILLIFDAKTLVDMSMFSTLIAYFLLYLAVFKESSGKIKAISLISMLITGLLILFRVYNFIIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58599</accession>
    <name>T2M7_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative type II restriction enzyme MjaORF1200P</fullName>
            <shortName evidence="1">R.MjaORF1200P</shortName>
            <ecNumber>3.1.21.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1199</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A putative type II restriction enzyme, its methylase would be M.MjaORF1200P (AC Q58600).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.21.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">To A.pernix APE2001.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64449">
        <property type="entry name" value="F64449"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58599"/>
    <dbReference type="SMR" id="Q58599"/>
    <dbReference type="STRING" id="243232.MJ_1199"/>
    <dbReference type="REBASE" id="4871">
        <property type="entry name" value="MjaORF1200P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99204">
        <property type="protein sequence ID" value="AAB99204"/>
        <property type="gene ID" value="MJ_1199"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1199"/>
    <dbReference type="eggNOG" id="arCOG01019">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140136_0_0_2"/>
    <dbReference type="InParanoid" id="Q58599"/>
    <dbReference type="OMA" id="LFKLAFK"/>
    <dbReference type="PhylomeDB" id="Q58599"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018665">
        <property type="entry name" value="DUF2122_RecB-nuclease-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09895">
        <property type="entry name" value="DUF2122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000107212" description="Putative type II restriction enzyme MjaORF1200P">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="146" mass="16296" checksum="9934CB449198D85C" modified="1996-11-01" version="1">MRKMFICLHNTYSAKQVEEFGRIAYGFDINTIVVTKATASAAQSGIPTLHKMAYKLGKNVLFFEELDDAIEVLRPEKVFLIGNKSICDEKVDFNEVGENDLVVFCGASTGFTKLELEKGLGRYIVENEIGALGNLAIFLYEMSKKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58602</accession>
    <name>Y1205_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0058 protein MJ1205</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1205</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0058 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64450">
        <property type="entry name" value="D64450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870717.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58602"/>
    <dbReference type="SMR" id="Q58602"/>
    <dbReference type="STRING" id="243232.MJ_1205"/>
    <dbReference type="EnsemblBacteria" id="AAB99209">
        <property type="protein sequence ID" value="AAB99209"/>
        <property type="gene ID" value="MJ_1205"/>
    </dbReference>
    <dbReference type="GeneID" id="1452100"/>
    <dbReference type="KEGG" id="mja:MJ_1205"/>
    <dbReference type="eggNOG" id="arCOG02254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167318_1_0_2"/>
    <dbReference type="InParanoid" id="Q58602"/>
    <dbReference type="OMA" id="HRSKAEH"/>
    <dbReference type="OrthoDB" id="121332at2157"/>
    <dbReference type="PhylomeDB" id="Q58602"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="PTHR42203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="SSF140371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135710" description="UPF0058 protein MJ1205">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="103" mass="12378" checksum="A7EEE4737597499E" modified="1996-11-01" version="1">MHKDELIQLHQLLIYLRKYIEKKYNCDNNEFKEYDELNIYPHHIHRTKAEHIYTIFLLSSIIAKILSDNGKIPRSVSNLLRVSGEKIKKEIQRKRCKIKNTNT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58603</accession>
    <name>DNAG_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase DnaG</fullName>
            <ecNumber evidence="1">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dnaG</name>
        <name type="ordered locus">MJ1206</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a ternary complex with MCM helicase and DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64450">
        <property type="entry name" value="E64450"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58603"/>
    <dbReference type="SMR" id="Q58603"/>
    <dbReference type="STRING" id="243232.MJ_1206"/>
    <dbReference type="EnsemblBacteria" id="AAB99210">
        <property type="protein sequence ID" value="AAB99210"/>
        <property type="gene ID" value="MJ_1206"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1206"/>
    <dbReference type="eggNOG" id="arCOG04281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034626_0_0_2"/>
    <dbReference type="InParanoid" id="Q58603"/>
    <dbReference type="OMA" id="KYMIVAQ"/>
    <dbReference type="PhylomeDB" id="Q58603"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000144125" description="DNA primase DnaG">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="171"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <sequence length="424" mass="47388" checksum="C55BC7A05B0EB2C3" modified="1996-11-01" version="1">MIIMDLGTTKYIIYAELIADGYVEKHDVIGAIFGQTEGLLGDELDLRELQKTGRVGRIDVELTNINGKSIAKITVPSSLDRIETSILAATLETIDRVGPCVATVKVIDIEDIRKKKREYIVERAKEILKQLMSNIDVNTIIEEVKESVRMGEIIEYGPERLPAGPAVDSSDDIIVVEGRADVLNLLRCGIKNVIAVEGTSVPKTIIELSKKKIVTVFTDGDRGGELILKELLQVCDVDFVARAPPGKEVEELSKKEIMKCLRSKIPAEHILAQILKDKQKIDEKVCKDEIRNMGIQTIPEIKPEISITSNDDVEVSSVECNPSNNEELPPKYNKYRKFYEKLIELEDSKVLIINGDKEEIVSIEELINNTDNYKSIDAIIINGTVTQKLIDILYEKTNLIFCKDAKIIKKPVNLTLITFGDLNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58612</accession>
    <name>Y1215_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ1215</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ1215</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1215</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable antitoxin component of a putative type VII toxin-antitoxin (TA) system. Neutralizes cognate toxic MJ1216 by di-AMPylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Probably forms a complex with cognate toxin MJ1216.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64451">
        <property type="entry name" value="F64451"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58612"/>
    <dbReference type="SMR" id="Q58612"/>
    <dbReference type="STRING" id="243232.MJ_1215"/>
    <dbReference type="EnsemblBacteria" id="AAB99216">
        <property type="protein sequence ID" value="AAB99216"/>
        <property type="gene ID" value="MJ_1215"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1215"/>
    <dbReference type="eggNOG" id="arCOG01206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_10_3_2"/>
    <dbReference type="InParanoid" id="Q58612"/>
    <dbReference type="OMA" id="AHELENY"/>
    <dbReference type="PhylomeDB" id="Q58612"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107218" description="Putative protein adenylyltransferase MJ1215">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="86" mass="10281" checksum="CD9296D0B135FD14" modified="1997-11-01" version="1">MRGRMKTLSEIKEILRKHKKELKENYKVKSIAIFGSYARGEQKETSDIDIMVEFYETPDYLKFFELEDYLENILNIKVDLITKNSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58644</accession>
    <name>PHI_METJA</name>
    <protein>
        <recommendedName>
            <fullName>3-hexulose-6-phosphate isomerase</fullName>
            <ecNumber>5.3.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>6-phospho-3-hexuloisomerase</fullName>
            <shortName>PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">phi</name>
        <name type="ordered locus">MJ1247</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Bacteriol." volume="187" first="7382" last="7389">
            <title>Ribose-5-phosphate biosynthesis in Methanocaldococcus jannaschii occurs in the absence of a pentose-phosphate pathway.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="16237021"/>
            <dbReference type="DOI" id="10.1128/JB.187.21.7382-7389.2005"/>
        </citation>
        <scope>PATHWAY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="Structure" volume="10" first="195" last="204">
            <title>Crystal structure of MJ1247 protein from M. jannaschii at 2.0 A resolution infers a molecular function of 3-hexulose-6-phosphate isomerase.</title>
            <authorList>
                <person name="Martinez-Cruz L.A."/>
                <person name="Dreyer M.K."/>
                <person name="Boisvert D.C."/>
                <person name="Yokota H."/>
                <person name="Martinez-Chantar M.L."/>
                <person name="Kim R."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="11839305"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(02)00701-3"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH CITRIC ACID</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the isomerization between 3-hexulose 6-phosphate and fructose 6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>D-arabino-hex-3-ulose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:25900"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58542"/>
            <dbReference type="EC" id="5.3.1.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.27"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64455">
        <property type="entry name" value="F64455"/>
    </dbReference>
    <dbReference type="PDB" id="1JEO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-180"/>
    </dbReference>
    <dbReference type="PDBsum" id="1JEO"/>
    <dbReference type="AlphaFoldDB" id="Q58644"/>
    <dbReference type="SMR" id="Q58644"/>
    <dbReference type="STRING" id="243232.MJ_1247"/>
    <dbReference type="EnsemblBacteria" id="AAB99251">
        <property type="protein sequence ID" value="AAB99251"/>
        <property type="gene ID" value="MJ_1247"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1247"/>
    <dbReference type="eggNOG" id="arCOG00068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094236_1_1_2"/>
    <dbReference type="InParanoid" id="Q58644"/>
    <dbReference type="OMA" id="KTIQPMG"/>
    <dbReference type="PhylomeDB" id="Q58644"/>
    <dbReference type="BRENDA" id="5.3.1.27">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00293"/>
    <dbReference type="EvolutionaryTrace" id="Q58644"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043800">
        <property type="term" value="F:hexulose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="PTHR43443"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03127">
        <property type="entry name" value="RuMP_HxlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136568" description="3-hexulose-6-phosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="33"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="90"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="5"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="16"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="19"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="41"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="69"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="85"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="96"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="127"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="169"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11839305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16237021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1JEO"/>
        </source>
    </evidence>
    <sequence length="180" mass="20443" checksum="7C3D607BCBD4AA0A" modified="1996-11-01" version="1">MSKLEELDIVSNNILILKKFYTNDEWKNKLDSLIDRIIKAKKIFIFGVGRSGYIGRCFAMRLMHLGFKSYFVGETTTPSYEKDDLLILISGSGRTESVLTVAKKAKNINNNIIAIVCECGNVVEFADLTIPLEVKKSKYLPMGTTFEETALIFLDLVIAEIMKRLNLDESEIIKRHCNLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58665</accession>
    <name>LIVH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable branched-chain amino acid transport permease protein LivH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">livH</name>
        <name type="ordered locus">MJ1269</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64458">
        <property type="entry name" value="D64458"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58665"/>
    <dbReference type="STRING" id="243232.MJ_1269"/>
    <dbReference type="EnsemblBacteria" id="AAB99275">
        <property type="protein sequence ID" value="AAB99275"/>
        <property type="gene ID" value="MJ_1269"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1269"/>
    <dbReference type="eggNOG" id="arCOG01269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039929_1_0_2"/>
    <dbReference type="InParanoid" id="Q58665"/>
    <dbReference type="OMA" id="NMGWFLI"/>
    <dbReference type="PhylomeDB" id="Q58665"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0029">Amino-acid transport</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060064" description="Probable branched-chain amino acid transport permease protein LivH">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="315" mass="33655" checksum="9AA352698413760A" modified="1996-11-01" version="1">MVKLLLKDLGEKMILEGAIIYSNLLVLLALGLTLTYITTNVPNFAQGSYAIVGSYVALTLLKLFGICPYLSLPVLFVVGAIVGLITYLALKPLIKRNASVEILMIATLAIDLILLGVIGAYSEILSQIVGSTQAKFVFANLDFSLFGFKGILFVSTFVVILLLIGLYLLLYKTKFGIALRASMENPSLAQTMGIDVEKTRLFSWILSGALAGVAGGLLPFMQEIVPATGDLIIISIFAASIVGGLRHISGALIGGYIIGISESLITYYLASAFGTGFLVYGKVISLIIMIATLLIAPYGITGVDWKKLKRLLSTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58685</accession>
    <name>Y1289_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1289</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1289</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64460">
        <property type="entry name" value="H64460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870805.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58685"/>
    <dbReference type="STRING" id="243232.MJ_1289"/>
    <dbReference type="EnsemblBacteria" id="AAB99295">
        <property type="protein sequence ID" value="AAB99295"/>
        <property type="gene ID" value="MJ_1289"/>
    </dbReference>
    <dbReference type="GeneID" id="1452190"/>
    <dbReference type="KEGG" id="mja:MJ_1289"/>
    <dbReference type="eggNOG" id="arCOG08272">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1607184_0_0_2"/>
    <dbReference type="OMA" id="SIIAYEW"/>
    <dbReference type="OrthoDB" id="110581at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107256" description="Uncharacterized protein MJ1289">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="165" mass="19092" checksum="8D4833D9C8DDB10D" modified="1996-11-01" version="1">MNTYLSTLLVLTTIFALSIIAYEWGINIIDTTLNQVSKEKEKNRIEIIKNLINDVIYSGVDSERTFDETKITLKENNVKISNGTKYVSFNITTIEGIDYDVYLEKGTLYIFIYNISAQCPNVYYIKYTNLSIYEFGGNITINYSDNLRHFYVNNSKVYVYSLLMG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58716</accession>
    <name>VAPC4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease VapC4</fullName>
            <shortName evidence="1">RNase VapC4</shortName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Putative toxin VapC4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">vapC4</name>
        <name type="ordered locus">MJ1320</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB4.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64464">
        <property type="entry name" value="G64464"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870837.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58716"/>
    <dbReference type="SMR" id="Q58716"/>
    <dbReference type="STRING" id="243232.MJ_1320"/>
    <dbReference type="EnsemblBacteria" id="AAB99330">
        <property type="protein sequence ID" value="AAB99330"/>
        <property type="gene ID" value="MJ_1320"/>
    </dbReference>
    <dbReference type="GeneID" id="1452222"/>
    <dbReference type="KEGG" id="mja:MJ_1320"/>
    <dbReference type="eggNOG" id="arCOG04312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107892_1_0_2"/>
    <dbReference type="InParanoid" id="Q58716"/>
    <dbReference type="OMA" id="DEMIINY"/>
    <dbReference type="OrthoDB" id="118267at2157"/>
    <dbReference type="PhylomeDB" id="Q58716"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107274" description="Ribonuclease VapC4">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="4"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <sequence length="131" mass="15452" checksum="BD4589FBD5A56693" modified="1996-11-01" version="1">MYKIVPDTNFLIYVFKHKINFDYEIERALNTKFEIVILSPIKEELERLLKSRDLKGKEKLAVNLALAKIKNYKLVDYTANYADEAILNYAKENENVIVATNDKELKEKLMENNIPVMVVRQKKYFEIFGMV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58720</accession>
    <name>MOBB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative molybdopterin-guanine dinucleotide biosynthesis adapter protein</fullName>
            <shortName>MGD biosynthesis adapter protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Molybdenum cofactor biosynthesis adapter protein</fullName>
            <shortName>Moco biosynthesis adapter protein</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mobB</name>
        <name type="ordered locus">MJ1324</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">GTP-binding protein that is not required for the biosynthesis of Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor, and not necessary for the formation of active molybdoenzymes using this form of molybdenum cofactor. May act as an adapter protein to achieve the efficient biosynthesis and utilization of MGD. Displays a weak intrinsic GTPase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MobB family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64465">
        <property type="entry name" value="C64465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870841.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58720"/>
    <dbReference type="SMR" id="Q58720"/>
    <dbReference type="STRING" id="243232.MJ_1324"/>
    <dbReference type="PRIDE" id="Q58720"/>
    <dbReference type="EnsemblBacteria" id="AAB99333">
        <property type="protein sequence ID" value="AAB99333"/>
        <property type="gene ID" value="MJ_1324"/>
    </dbReference>
    <dbReference type="GeneID" id="1452226"/>
    <dbReference type="KEGG" id="mja:MJ_1324"/>
    <dbReference type="eggNOG" id="arCOG00532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068199_0_1_2"/>
    <dbReference type="InParanoid" id="Q58720"/>
    <dbReference type="OMA" id="HCGYNCK"/>
    <dbReference type="OrthoDB" id="62565at2157"/>
    <dbReference type="PhylomeDB" id="Q58720"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107276" description="Putative molybdopterin-guanine dinucleotide biosynthesis adapter protein">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S" evidence="3">
        <location>
            <begin position="134"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="42"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="234" mass="26080" checksum="F79BB9B1642A5E1B" modified="1996-11-01" version="1">MRVIGVIGYKDSGKTTLIEEILKHSDKKIAVIKHTKEDVEVDKKGTDTYRLSNAGAKITVLATDSKTVFFTDRMDLENILSVLSDYNIDFVIIEGFKEALKRLNIPKIVMLKDKDGSDLIDDHTAMIIEDYNYNIDDVLKVIYEKAVVPTMNLNCGHCGYNCRTFVKAVVKGEARWDDCVLAKGIKIIVDGKIIPAVPFVSKIVGSTIKAMIETLKGVDNPKTIKVEIDVSKLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58723</accession>
    <name>T2M4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type II restriction enzyme MjaIV</fullName>
            <shortName>R.MjaIV</shortName>
            <ecNumber>3.1.21.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Endonuclease MjaIV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Type-2 restriction enzyme MjaIV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaIVR</name>
        <name type="ordered locus">MJ1327</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="patent" date="1999-03-11" number="WO9911821">
            <title>Method for screening restriction endonucleases.</title>
            <authorList>
                <person name="Noren C.J."/>
                <person name="Roberts R.J."/>
                <person name="Patti J."/>
                <person name="Byrd D.R."/>
                <person name="Morgan R.D."/>
            </authorList>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GTNNAC-3'; the cleavage site is unknown.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.21.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.21.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64465">
        <property type="entry name" value="F64465"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58723"/>
    <dbReference type="STRING" id="243232.MJ_1327"/>
    <dbReference type="REBASE" id="3894">
        <property type="entry name" value="MjaIV"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99343">
        <property type="protein sequence ID" value="AAB99343"/>
        <property type="gene ID" value="MJ_1327"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1327"/>
    <dbReference type="HOGENOM" id="CLU_1131612_0_0_2"/>
    <dbReference type="BRENDA" id="3.1.21.4">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="PRO" id="PR:Q58723"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000077335" description="Type II restriction enzyme MjaIV">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source ref="2"/>
    </evidence>
    <sequence length="245" mass="28779" checksum="4597C2B8EF1FA973" modified="1996-11-01" version="1">MVVKLVNNELKILSGKLRDIFREIYNKIKNDENINDRNLDDKVLSLLKDYTISEENLKIKFSEPKDEIYSYEGRRTPYDLLCYGIINGKNFLIFINNKFGDLKSNTRNDVTTYNNLLRLYLGIKRQRLTSEITINGELVYNRISGNEIVSYGIFVVDKYRRGYKFFLLEEIKDDFYVNPRNNMFQIRYSPNLGDPIDYFAFVKKLIDAILESLEKSLNSIKTEILVLNSIKIQLINIKEGKHGED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-04" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58756</accession>
    <name>Y1361_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1361</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1361</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64469">
        <property type="entry name" value="H64469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870878.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58756"/>
    <dbReference type="STRING" id="243232.MJ_1361"/>
    <dbReference type="EnsemblBacteria" id="AAB99369">
        <property type="protein sequence ID" value="AAB99369"/>
        <property type="gene ID" value="MJ_1361"/>
    </dbReference>
    <dbReference type="GeneID" id="1452263"/>
    <dbReference type="KEGG" id="mja:MJ_1361"/>
    <dbReference type="eggNOG" id="arCOG02715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_965090_0_0_2"/>
    <dbReference type="OMA" id="WSKYLWG"/>
    <dbReference type="OrthoDB" id="73785at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012030">
        <property type="entry name" value="UCP006563"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006563">
        <property type="entry name" value="UCP006563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107298" description="Uncharacterized protein MJ1361">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="292" mass="33193" checksum="4F4DC241760F1A36" modified="1996-11-01" version="1">MRAYPIKTRYIKRGENFIPIVVEAIKNSGIKLEDGDFVVLSEKMVSTAEGNFIDESKFKPGVLAYLCYYWSKYLWGYVLGKLLKVKEDKIKNLRRMPKEETLKHKQTIIEIVGLRYALKPYAEGGVDLTNVPGTYACPLPKNPKKWAEELYKEIKKELGVDVVVMVADTDATYRVLNFYFTALPYAIDGIISGIGVFGFILGRLADVLKIGGFAGCTPLAIAGNEVYKKYSIGELTRIAFICDRVHKTIKNINEVLEKYNTYVITEEILEKLEHTPVVVVKIKEEYKPESQR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58771</accession>
    <name>RLME_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal RNA large subunit methyltransferase E</fullName>
            <ecNumber evidence="1">2.1.1.166</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">23S rRNA Um2552 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">rRNA (uridine-2'-O-)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rlmE</name>
        <name evidence="1" type="synonym">rrmJ</name>
        <name type="ordered locus">MJ1376</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O-methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42720"/>
            <dbReference type="Rhea" id="RHEA-COMP:10202"/>
            <dbReference type="Rhea" id="RHEA-COMP:10203"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74478"/>
            <dbReference type="EC" id="2.1.1.166"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.166" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64471">
        <property type="entry name" value="G64471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870893.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58771"/>
    <dbReference type="SMR" id="Q58771"/>
    <dbReference type="STRING" id="243232.MJ_1376"/>
    <dbReference type="PRIDE" id="Q58771"/>
    <dbReference type="EnsemblBacteria" id="AAB99383">
        <property type="protein sequence ID" value="AAB99383"/>
        <property type="gene ID" value="MJ_1376"/>
    </dbReference>
    <dbReference type="GeneID" id="1452279"/>
    <dbReference type="KEGG" id="mja:MJ_1376"/>
    <dbReference type="eggNOG" id="arCOG00079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009422_4_4_2"/>
    <dbReference type="InParanoid" id="Q58771"/>
    <dbReference type="OMA" id="HRQTDHL"/>
    <dbReference type="OrthoDB" id="105478at2157"/>
    <dbReference type="PhylomeDB" id="Q58771"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008173">
        <property type="term" value="F:RNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008650">
        <property type="term" value="F:rRNA (uridine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01547">
        <property type="entry name" value="RNA_methyltr_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015507">
        <property type="entry name" value="rRNA-MeTfrase_E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004512">
        <property type="entry name" value="rRNA_MeTrfase_gammaproteobac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005461">
        <property type="entry name" value="23S_rRNA_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00438">
        <property type="entry name" value="rrmJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155567" description="Ribosomal RNA large subunit methyltransferase E">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01547"/>
        </source>
    </evidence>
    <sequence length="245" mass="28559" checksum="64C0FB31726FFD97" modified="1996-11-01" version="1">MGRKDKRWVLQRKRDFYYKLAKKLKYRSRASFKLMQLNEKFNVIKPGKIVLDLGCAPGGWMQVAREIVGDKGFVIGIDLQPVKPFEYDNVVAIKGDFTLEENLNKIRELIPNDEKKVDVVISDASPNISGYWDIDHARSIDLVTTALQIATEMLKERGNFVAKVFYGDMIDDYVNLVKKYFEKVYITKPQASRKESAEVYVIAKRYTGKKWEEEDKIKRVKKVENEDNELLAKKIKEIRKLKSKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58797</accession>
    <name>Y1402_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1402</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1402</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64475">
        <property type="entry name" value="A64475"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870919.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58797"/>
    <dbReference type="EnsemblBacteria" id="AAB99412">
        <property type="protein sequence ID" value="AAB99412"/>
        <property type="gene ID" value="MJ_1402"/>
    </dbReference>
    <dbReference type="GeneID" id="1452305"/>
    <dbReference type="KEGG" id="mja:MJ_1402"/>
    <dbReference type="eggNOG" id="arCOG05987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775263_0_0_2"/>
    <dbReference type="OMA" id="AYLLCTN"/>
    <dbReference type="OrthoDB" id="46160at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014015" description="Uncharacterized protein MJ1402">
        <location>
            <begin position="29"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="346" mass="38064" checksum="46034EA32D0F69DF" modified="1996-11-01" version="1" precursor="true">MFEWMKNKKAISPILALLIVLGVTIVVGAVFYAWGSGLFNNSQQSTQSALEGTTSTITYAAGAIGVGVPKEIDVEGDLDLTYPTPDYKLSHLTTTDYGSYDERLIVPVPLTLENYYDSTLTNVKIESDGATEVAGLTLKKITLNYNGQNYDAYLLCTNDGTPFKGILNRTGIYPDATWTGDDGNNYTSVYYILAPNSVTGVAAVDGSKDLSVTTAKKWPYSQNDVQSMRLYAGGFNNMWYACAVNGSYSSWTNTLTATKFIGWNTAQAFYKYKTPIDAKFYTSEWDVGTLHKGEKVSKEIFFFFGSSMGFQEEPSGETTVKIPVKVVSDQGVYKQVDVNIVLKDRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58808</accession>
    <name>Y1413_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0145 protein MJ1413</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1413</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0145 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64476">
        <property type="entry name" value="D64476"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58808"/>
    <dbReference type="SMR" id="Q58808"/>
    <dbReference type="STRING" id="243232.MJ_1413"/>
    <dbReference type="EnsemblBacteria" id="AAB99420">
        <property type="protein sequence ID" value="AAB99420"/>
        <property type="gene ID" value="MJ_1413"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1413"/>
    <dbReference type="eggNOG" id="arCOG02287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117144_3_2_2"/>
    <dbReference type="InParanoid" id="Q58808"/>
    <dbReference type="OMA" id="MSADIKM"/>
    <dbReference type="PhylomeDB" id="Q58808"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00338">
        <property type="entry name" value="UPF0145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002765">
        <property type="entry name" value="UPF0145"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035439">
        <property type="entry name" value="UPF0145_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34068">
        <property type="entry name" value="PTHR34068"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01906">
        <property type="entry name" value="YbjQ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117782">
        <property type="entry name" value="SSF117782"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138495" description="UPF0145 protein MJ1413">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="103" mass="11233" checksum="77029876B2B94E17" modified="1996-11-01" version="1">MGGMITTTTPYIEGKKIIKYLGFVHGVASVYVTVKYYEDVKDAYERALRESEDTALIRMVDNAKKLGANAIIGINSNYAMVGEKGDMIMVGIYGTAVVVEEDG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58836</accession>
    <name>Y1441_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1441</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1441</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunit H family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64479">
        <property type="entry name" value="H64479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870959.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58836"/>
    <dbReference type="SMR" id="Q58836"/>
    <dbReference type="STRING" id="243232.MJ_1441"/>
    <dbReference type="EnsemblBacteria" id="AAB99452">
        <property type="protein sequence ID" value="AAB99452"/>
        <property type="gene ID" value="MJ_1441"/>
    </dbReference>
    <dbReference type="GeneID" id="1452345"/>
    <dbReference type="KEGG" id="mja:MJ_1441"/>
    <dbReference type="eggNOG" id="arCOG03022">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002017_1_0_2"/>
    <dbReference type="InParanoid" id="Q58836"/>
    <dbReference type="OMA" id="YQFQGGL"/>
    <dbReference type="OrthoDB" id="344at2157"/>
    <dbReference type="PhylomeDB" id="Q58836"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="PTHR44119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000219888" description="Uncharacterized protein MJ1441">
        <location>
            <begin position="1"/>
            <end position="1226"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="1226" mass="141328" checksum="8F7DDF4E36162AB9" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58837</accession>
    <name>Y1442_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1442</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1442</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64480">
        <property type="entry name" value="A64480"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58837"/>
    <dbReference type="STRING" id="243232.MJ_1442"/>
    <dbReference type="EnsemblBacteria" id="AAB99459">
        <property type="protein sequence ID" value="AAB99459"/>
        <property type="gene ID" value="MJ_1442"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1442"/>
    <dbReference type="HOGENOM" id="CLU_1352121_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107335" description="Uncharacterized protein MJ1442">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="202" mass="22658" checksum="B9158361B5E6D9FC" modified="1996-11-01" version="1">MTSIRIFIKFYGGTMRKFLIFLIFLSVLGCGITISGCIGGKNVEEIQNMQEQVVQQQQNENQEEYQNEDEGVDYNSIRDVQPIGTAKEADEKIRPILNEVFGEVKLMEYVSTGKQNEGESIVLTYVPKRKITTNDFEKLNEAIKKSGYFESSGGIAGGGQSGEGMVLWYVSKDNKSAIQIILYPDTNEIVVGYYKGKIYSSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58844</accession>
    <name>T2M2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II restriction enzyme MjaII</fullName>
            <shortName>R.MjaII</shortName>
            <ecNumber>3.1.21.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Endonuclease MjaII</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Type-2 restriction enzyme MjaII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaIIR</name>
        <name type="ordered locus">MJ1449</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="patent" date="1999-03-11" number="WO9911821">
            <title>Method for screening restriction endonucleases.</title>
            <authorList>
                <person name="Noren C.J."/>
                <person name="Roberts R.J."/>
                <person name="Patti J."/>
                <person name="Byrd D.R."/>
                <person name="Morgan R.D."/>
            </authorList>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GGNCC-3'; the cleavage site is unknown.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.21.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TdeIII type II restriction endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64480">
        <property type="entry name" value="H64480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870969.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58844"/>
    <dbReference type="STRING" id="243232.MJ_1449"/>
    <dbReference type="REBASE" id="1222">
        <property type="entry name" value="MjaII"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99461">
        <property type="protein sequence ID" value="AAB99461"/>
        <property type="gene ID" value="MJ_1449"/>
    </dbReference>
    <dbReference type="GeneID" id="1452353"/>
    <dbReference type="KEGG" id="mja:MJ_1449"/>
    <dbReference type="eggNOG" id="arCOG07628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_756000_0_0_2"/>
    <dbReference type="InParanoid" id="Q58844"/>
    <dbReference type="OMA" id="PNAGEMR"/>
    <dbReference type="OrthoDB" id="58569at2157"/>
    <dbReference type="PRO" id="PR:Q58844"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019045">
        <property type="entry name" value="Restrct_endonuc_II_TdeIII"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09520">
        <property type="entry name" value="RE_TdeIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000077333" description="Type II restriction enzyme MjaII">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="370" mass="43911" checksum="8B4CF6208F914B33" modified="1996-11-01" version="1">MPLSKNVIEKISIETIRVLKSRFDTISDEDIKIRNMPFHMAFLRAFYGKIGINDDTEALKFLTLSQWFHGLSTTLGQSYFENIAHILSNGEKRTFKNYKIKRSVRDKISEIINDLKSGERLPNVEKENKELREATSKNSEYVNGLEFTADVYFEDKDKVVMIELKTVRPNAGEMRGEKQKILYGKAYMMETKPNKKVYYFIGFPYDPTENPENPCGYDKDRFMSSLIEFSKYFDKREVLIAEELWSFLSGEENTMKKILDIINSIAKPDFKEKFDFINTFPFINQDRLYTKDAIDEQKFKKYMDILQEWRLYSEIECAKAVKELSLLKLSSRDRRTFERLINNSMFSNNNKYNENRKMKILELYNKYMQK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58847</accession>
    <name>Y1452_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1452</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1452</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH738.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64481">
        <property type="entry name" value="C64481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870972.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58847"/>
    <dbReference type="SMR" id="Q58847"/>
    <dbReference type="STRING" id="243232.MJ_1452"/>
    <dbReference type="EnsemblBacteria" id="AAB99463">
        <property type="protein sequence ID" value="AAB99463"/>
        <property type="gene ID" value="MJ_1452"/>
    </dbReference>
    <dbReference type="GeneID" id="1452356"/>
    <dbReference type="KEGG" id="mja:MJ_1452"/>
    <dbReference type="eggNOG" id="arCOG04843">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1067987_0_0_2"/>
    <dbReference type="InParanoid" id="Q58847"/>
    <dbReference type="OMA" id="VPQWHYS"/>
    <dbReference type="OrthoDB" id="77659at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018216">
        <property type="term" value="P:peptidyl-arginine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021172">
        <property type="entry name" value="UCP006607_RNA_methylase-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PTHR11006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006607">
        <property type="entry name" value="RNAmts_UCP006607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107340" description="Uncharacterized protein MJ1452">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="259" mass="29428" checksum="F1EC01380F09376E" modified="1996-11-01" version="1">MKLRLKVPQWHYSLLTDYERLAIFKNAIERVVDEDDVVFDLGTGSGILAMIAAKKAKKVYAIELDPFTYDYAKENIKVNGFNNIEIIEGDASTYNFKEKADVVIAELLDTALIIEPQVKVMNSIIERGFLKEDVKIIPAKAISTIQLVEAKMSHIYYDEDIKSEEVSEEVIYEEVDFHKTNPIEVSYNIELELEKSCENLGIKLRTYTILDDKHVAGQTSMLNPPLVIPLNKKVDKGRVKINLSYRRGGDLESIKVNLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58863</accession>
    <name>Y1468_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1468</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1468</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64483">
        <property type="entry name" value="C64483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870988.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58863"/>
    <dbReference type="SMR" id="Q58863"/>
    <dbReference type="STRING" id="243232.MJ_1468"/>
    <dbReference type="EnsemblBacteria" id="AAB99478">
        <property type="protein sequence ID" value="AAB99478"/>
        <property type="gene ID" value="MJ_1468"/>
    </dbReference>
    <dbReference type="GeneID" id="1452372"/>
    <dbReference type="KEGG" id="mja:MJ_1468"/>
    <dbReference type="eggNOG" id="arCOG02508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_294353_0_0_2"/>
    <dbReference type="InParanoid" id="Q58863"/>
    <dbReference type="OMA" id="WCGYLNN"/>
    <dbReference type="OrthoDB" id="2301at2157"/>
    <dbReference type="PhylomeDB" id="Q58863"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026495">
        <property type="entry name" value="PIP_CTERM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="SSF49299"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04173">
        <property type="entry name" value="PIP_CTERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107354" description="Uncharacterized protein MJ1468">
        <location>
            <begin position="1"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="PKD 1" evidence="2">
        <location>
            <begin position="213"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="PKD 2" evidence="2">
        <location>
            <begin position="436"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="PKD 3" evidence="2">
        <location>
            <begin position="724"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="PKD 4" evidence="2">
        <location>
            <begin position="822"/>
            <end position="886"/>
        </location>
    </feature>
    <feature type="domain" description="PKD 5" evidence="2">
        <location>
            <begin position="925"/>
            <end position="962"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1009" mass="115120" checksum="13E9B4933EAB7972" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58864</accession>
    <name>Y1469_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0333 protein MJ1469</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1469</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0333 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64483">
        <property type="entry name" value="D64483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870989.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58864"/>
    <dbReference type="SMR" id="Q58864"/>
    <dbReference type="STRING" id="243232.MJ_1469"/>
    <dbReference type="EnsemblBacteria" id="AAB99484">
        <property type="protein sequence ID" value="AAB99484"/>
        <property type="gene ID" value="MJ_1469"/>
    </dbReference>
    <dbReference type="GeneID" id="1452373"/>
    <dbReference type="KEGG" id="mja:MJ_1469"/>
    <dbReference type="eggNOG" id="arCOG09683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197251_1_0_2"/>
    <dbReference type="OMA" id="SIVGFYY"/>
    <dbReference type="OrthoDB" id="131326at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026451">
        <property type="entry name" value="ClassIII_w_PIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04205">
        <property type="entry name" value="classIII_w_PIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000218269" description="UPF0333 protein MJ1469">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="75" mass="8050" checksum="8F4FDE15923B9ED7" modified="1996-11-01" version="1">MKPKKIISNKAQISLELALLLGALVVAASIVGFYYLKSVTRGTSTAESISKNITLAAKNKALDNIYKVKRALNGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58889</accession>
    <name>PRS2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative 26S proteasome regulatory subunit homolog MJ1494</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1494</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871017.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58889"/>
    <dbReference type="SMR" id="Q58889"/>
    <dbReference type="STRING" id="243232.MJ_1494"/>
    <dbReference type="PRIDE" id="Q58889"/>
    <dbReference type="EnsemblBacteria" id="AAB99505">
        <property type="protein sequence ID" value="AAB99505"/>
        <property type="gene ID" value="MJ_1494"/>
    </dbReference>
    <dbReference type="GeneID" id="1452401"/>
    <dbReference type="KEGG" id="mja:MJ_1494"/>
    <dbReference type="eggNOG" id="arCOG04163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_3_2"/>
    <dbReference type="InParanoid" id="Q58889"/>
    <dbReference type="OMA" id="FGEWAPK"/>
    <dbReference type="OrthoDB" id="41252at2157"/>
    <dbReference type="PhylomeDB" id="Q58889"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000084754" description="Putative 26S proteasome regulatory subunit homolog MJ1494">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="161"/>
            <end position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="371" mass="42167" checksum="01903F231167E85E" modified="1998-12-15" version="2">MSKIGFNPIKIKSFSKIKTYDDTLPSLKYVVLEPAGFPIRVSSENVKVSTDDPILFNIYARDQWIGEIVKEGDYLFDNSILPDYAFKVISTYPKEGGMITSETVFKLQTPKKVLRTQFKKAKFSEIIGQEEAKKKCRIIMKYLENPKLFGEWAPKNVLFYGPPGTGKTLMARALATETNSSFILVKAPELIGEHVGDASKMIRELYQRASESAPCIVFIDELDAIGLSREYQSLRGDVSEVVNALLTELDGIKENEGVVTIAATNNPAMLDPAIRSRFEEEIEFKLPNDEERLKIMELYAKKMPLPVKANLKEFVEKTKGFSGRDIKEKFLKPALHRAILEDRDYVSKEDLEWALKKILGNRREAPQHLYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58895</accession>
    <name>T2M5_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II restriction enzyme MjaV</fullName>
            <shortName>R.MjaV</shortName>
            <ecNumber>3.1.21.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Endonuclease MjaV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Type-2 restriction enzyme MjaV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaVR</name>
        <name type="ordered locus">MJ1500</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="patent" date="1999-03-11" number="WO9911821">
            <title>Method for screening restriction endonucleases.</title>
            <authorList>
                <person name="Noren C.J."/>
                <person name="Roberts R.J."/>
                <person name="Patti J."/>
                <person name="Byrd D.R."/>
                <person name="Morgan R.D."/>
            </authorList>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GTAC-3'; the cleavage site is unknown.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.21.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.21.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64487">
        <property type="entry name" value="C64487"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871023.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58895"/>
    <dbReference type="STRING" id="243232.MJ_1500"/>
    <dbReference type="REBASE" id="3898">
        <property type="entry name" value="MjaV"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99521">
        <property type="protein sequence ID" value="AAB99521"/>
        <property type="gene ID" value="MJ_1500"/>
    </dbReference>
    <dbReference type="GeneID" id="1452407"/>
    <dbReference type="KEGG" id="mja:MJ_1500"/>
    <dbReference type="eggNOG" id="arCOG08288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104917_0_0_2"/>
    <dbReference type="OMA" id="HRTFYAS"/>
    <dbReference type="OrthoDB" id="66837at2157"/>
    <dbReference type="BRENDA" id="3.1.21.4">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="PRO" id="PR:Q58895"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000077336" description="Type II restriction enzyme MjaV">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <sequence length="230" mass="26764" checksum="9566FE2061C144D5" modified="1998-06-01" version="2">MDDKSYYEEIESILRQILQPIEKISFSTFIRVVSGYKIIPIDLSKKEDKELINDLAKACNEVIEEIKKTGGVKTKEGKTPKRVNEVGNHIEHYVKDVLNKYGYAITPKTKKGKQKSTGYPDIEFWYKGKKERDGRVVYIEIKTFNEKNINSSHRTFYASPSKDEEGVKIRYDAPHLCLSFKIEKLGRDYYATGFKIIDLSKLKGGIKREFNASNRELYKKDLIIYEKDLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58930</accession>
    <name>Y1535_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1535</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1535</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64491">
        <property type="entry name" value="F64491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871059.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58930"/>
    <dbReference type="SMR" id="Q58930"/>
    <dbReference type="EnsemblBacteria" id="AAB99558">
        <property type="protein sequence ID" value="AAB99558"/>
        <property type="gene ID" value="MJ_1535"/>
    </dbReference>
    <dbReference type="GeneID" id="1452443"/>
    <dbReference type="KEGG" id="mja:MJ_1535"/>
    <dbReference type="eggNOG" id="arCOG05088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982151_0_0_2"/>
    <dbReference type="OMA" id="YIGTFYA"/>
    <dbReference type="OrthoDB" id="71167at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107394" description="Uncharacterized protein MJ1535">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="299" mass="34958" checksum="C201F18230F66769" modified="1996-11-01" version="1">MKRLFFIFVMLIVLLCGCTSNKTTNVNNEININKIETNKTLENESLIQKTEKNQLAKIKEIKYMYIVKEGNKTKIQFALAYENGSLARVSNGKVVLKIFDDSGLLFNKTYYINELPLKAGYYYEIELPKIKGFYKNAKFVLTFKNDNVNLSKTTYGTIERYSEEEMKEIFEKEYHENSIKTNIEEFRDVVGIKFTVKEYGYYKIYNNQTDRIEKVFRVDFVAKNLNPDTYEFAPIGVCLISGDKKYWKIGGLDEIEIGINQEIAGYWIFNKPDSIENLRLDFKMGNIVYDIPLTQNNLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q58931</accession>
    <name>TRXB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative thioredoxin reductase</fullName>
            <shortName>TRXR</shortName>
            <ecNumber>1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1536</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="2">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The active site is a redox-active disulfide bond.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64491">
        <property type="entry name" value="G64491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871060.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58931"/>
    <dbReference type="SMR" id="Q58931"/>
    <dbReference type="STRING" id="243232.MJ_1536"/>
    <dbReference type="EnsemblBacteria" id="AAB99556">
        <property type="protein sequence ID" value="AAB99556"/>
        <property type="gene ID" value="MJ_1536"/>
    </dbReference>
    <dbReference type="GeneID" id="1452444"/>
    <dbReference type="KEGG" id="mja:MJ_1536"/>
    <dbReference type="eggNOG" id="arCOG01296">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_3_2"/>
    <dbReference type="InParanoid" id="Q58931"/>
    <dbReference type="OMA" id="VDNFPGY"/>
    <dbReference type="OrthoDB" id="41707at2157"/>
    <dbReference type="PhylomeDB" id="Q58931"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0676">Redox-active center</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000166759" description="Putative thioredoxin reductase">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="272"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="2">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P0A9P4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="301" mass="33035" checksum="B8319F2B33559AD8" modified="1996-11-01" version="1">MIHDTIIIGAGPGGLTAGIYAMRGKLNALCIEKENAGGRIAEAGIVENYPGFEEIRGYELAEKFKNHAEKFKLPIIYDEVIKIETKERPFKVITKNSEYLTKTIVIATGTKPKKLGLNEDKFIGRGISYCTMCDAFFYLNKEVIVIGRDTPAIMSAINLKDIAKKVIVITDKSELKAAESIMLDKLKEANNVEIIYNAKPLEIVGEERAEGVKISVNGKEEIIKADGIFISLGHVPNTEFLKDSGIELDKKGFIKTDENCRTNIDGIYAVGDVRGGVMQVAKAVGDGCVAMANIIKYLQKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58943</accession>
    <name>Y1548_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative RNase MJ1548</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative toxin MJ1548</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1548</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable toxic component of a putative type VII toxin-antitoxin (TA) system, probably an RNase. Probably neutralized by cognate antitoxin MJ1547. Neutralization may be due to AMPylation by antitoxin MJ1547.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer, probably forms a complex with cognate antitoxin MJ1547.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Modified by cognate antitoxin MJ1547; probably at least 2 successive AMPylation events occur on Tyr-72.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAB99566" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99566.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64493">
        <property type="entry name" value="C64493"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58943"/>
    <dbReference type="SMR" id="Q58943"/>
    <dbReference type="STRING" id="243232.MJ_1548"/>
    <dbReference type="EnsemblBacteria" id="AAB99566">
        <property type="protein sequence ID" value="AAB99566"/>
        <property type="gene ID" value="MJ_1548"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1548"/>
    <dbReference type="eggNOG" id="arCOG02109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152343_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58943"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037038">
        <property type="entry name" value="HepT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000158265" description="Putative RNase MJ1548">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RX(4)HXY motif" evidence="1">
        <location>
            <begin position="65"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-di-AMP-tyrosine" evidence="1">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJR1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="87" mass="10328" checksum="685075019C11F980" modified="2021-04-07" version="2">MRLQKGLYYISLQVCVDITMDVVAMLVKDIGLNVEDDYTNIKKLLKHDVITKDEATLLKQYNRLRNAIVHKYDKIKLRSCKRRFKKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q58958</accession>
    <name>Y1563_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative HTH-type transcriptional regulator MJ1563</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>DNA-binding protein MJ1563</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1563</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Proteins" volume="50" first="170" last="173">
            <title>X-ray structure of an M.jannaschii DNA-binding protein: implications for antibiotic resistance in S. aureus.</title>
            <authorList>
                <person name="Ray S.S."/>
                <person name="Bonanno J.B."/>
                <person name="Chen H."/>
                <person name="de Lencastre H."/>
                <person name="Wu S."/>
                <person name="Tomasz A."/>
                <person name="Burley S.K."/>
            </authorList>
            <dbReference type="PubMed" id="12471609"/>
            <dbReference type="DOI" id="10.1002/prot.10272"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS)</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the GbsR family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64495">
        <property type="entry name" value="B64495"/>
    </dbReference>
    <dbReference type="PDB" id="1KU9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-152"/>
    </dbReference>
    <dbReference type="PDBsum" id="1KU9"/>
    <dbReference type="AlphaFoldDB" id="Q58958"/>
    <dbReference type="SMR" id="Q58958"/>
    <dbReference type="STRING" id="243232.MJ_1563"/>
    <dbReference type="EnsemblBacteria" id="AAB99584">
        <property type="protein sequence ID" value="AAB99584"/>
        <property type="gene ID" value="MJ_1563"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1563"/>
    <dbReference type="eggNOG" id="arCOG02795">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107445_0_0_2"/>
    <dbReference type="InParanoid" id="Q58958"/>
    <dbReference type="OMA" id="KVWIKGE"/>
    <dbReference type="PhylomeDB" id="Q58958"/>
    <dbReference type="EvolutionaryTrace" id="Q58958"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026282">
        <property type="entry name" value="MJ1563"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006707">
        <property type="entry name" value="MJ1563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000160637" description="Putative HTH-type transcriptional regulator MJ1563">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="44"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="3"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="26"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="44"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="70"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="89"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="135"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1KU9"/>
        </source>
    </evidence>
    <sequence length="152" mass="17609" checksum="ABD121B907F4271A" modified="1996-11-01" version="1">MIIMEEAKKLIIELFSELAKIHGLNKSVGAVYAILYLSDKPLTISDIMEELKISKGNVSMSLKKLEELGFVRKVWIKGERKNYYEAVDGFSSIKDIAKRKHDLIAKTYEDLKKLEEKCNEEEKEFIKQKIKGIERMKKISEKILEALNDLDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q58967</accession>
    <name>Y1572_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ABC transporter ATP-binding protein MJ1572</fullName>
            <ecNumber>7.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1572</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EC" id="7.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64496">
        <property type="entry name" value="C64496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871096.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58967"/>
    <dbReference type="SMR" id="Q58967"/>
    <dbReference type="STRING" id="243232.MJ_1572"/>
    <dbReference type="EnsemblBacteria" id="AAB99589">
        <property type="protein sequence ID" value="AAB99589"/>
        <property type="gene ID" value="MJ_1572"/>
    </dbReference>
    <dbReference type="GeneID" id="1452480"/>
    <dbReference type="KEGG" id="mja:MJ_1572"/>
    <dbReference type="eggNOG" id="arCOG00202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q58967"/>
    <dbReference type="OMA" id="CSQIGMV"/>
    <dbReference type="OrthoDB" id="92866at2157"/>
    <dbReference type="PhylomeDB" id="Q58967"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092142" description="Putative ABC transporter ATP-binding protein MJ1572">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="2">
        <location>
            <begin position="5"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="278" mass="31726" checksum="E87C62B70AB114D2" modified="1996-11-01" version="1">MKEIYRLVDVSYKYPNGSIALDNVNLNIYKNEVVAILGPNGAGKTTLLKILDGLVFPDKGEVYFEGKKLTDEILRDKELMKEFRRKVGFVFQNPDVMLFNPTVWDEVAFSPLHLYSKEKAIEVTDKTLKDMKIYHLKDRHPYNLSGGEKKKVSISCILSVEPEVILMDEPTSALDPKSRAEIMNLIKSFKECGKTVVLVTHDLNLACLADRCYVLNKKVIFEGKVKDLFSLNLDELNLDVPEISKLFIKLKNMGYNINEIPVTLDEAVNIISKLFQKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q58992</accession>
    <name>GLYA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Serine hydroxymethyltransferase</fullName>
            <shortName evidence="1">SHMT</shortName>
            <shortName evidence="1">Serine methylase</shortName>
            <ecNumber evidence="1 2">2.1.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>L-allo-threonine aldolase</fullName>
            <ecNumber>4.1.2.49</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyA</name>
        <name type="ordered locus">MJ1597</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="41789" last="41797">
            <title>Catalytic and thermodynamic properties of tetrahydromethanopterin-dependent serine hydroxymethyltransferase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Angelaccio S."/>
                <person name="Chiaraluce R."/>
                <person name="Consalvi V."/>
                <person name="Buchenau B."/>
                <person name="Giangiacomo L."/>
                <person name="Bossa F."/>
                <person name="Contestabile R."/>
            </authorList>
            <dbReference type="PubMed" id="12902326"/>
            <dbReference type="DOI" id="10.1074/jbc.M306747200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>H4MPT-DEPENDENT SERINE HYDROXYMETHYLTRANSFERASE ACTIVITY</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ALDOLASE ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible interconversion of serine and glycine with tetrahydromethanopterin (H4MPT) serving as the one-carbon carrier. The use of tetrahydrofolate (THF or H4PteGlu) as the pteridine substrate is 450-fold less efficient than that of H4MPT. Also exhibits a pteridine-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism. Thus, is able to catalyze the cleavage of L-allo-threonine and L-threo-beta-phenylserine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>5,10-methylenetetrahydromethanopterin + glycine + H2O = 5,6,7,8-tetrahydromethanopterin + L-serine</text>
            <dbReference type="Rhea" id="RHEA:47104"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>L-allo-threonine = acetaldehyde + glycine</text>
            <dbReference type="Rhea" id="RHEA:26209"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58585"/>
            <dbReference type="EC" id="4.1.2.49"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.8 mM for L-serine (at 37 degrees Celsius)</KM>
            <KM evidence="2">0.1 mM for tetrahydromethanopterin (at 37 degrees Celsius)</KM>
            <KM evidence="2">1.3 mM for L-allo-threonine (at 60 degrees Celsius)</KM>
            <KM evidence="2">95 mM for DL-threo-beta-phenylserine (at 60 degrees Celsius)</KM>
            <text>kcat is 212 min(-1) for the L-serine hydroxymethyltransferase reaction, and 687 min(-1) for the L-allo-threonine cleavage, at 37 and 60 degrees Celsius, respectively. The presence of oxygen does not seem to affect significantly the kinetic parameters of the reactions.</text>
        </kinetics>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 70-80 degrees Celsius for the retro-aldol cleavage of L-allo-threonine.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SHMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.-" evidence="1 2"/>
    <dbReference type="EC" id="4.1.2.49"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64499">
        <property type="entry name" value="D64499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871121.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4BHD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.83 A"/>
        <property type="chains" value="A/B=3-429"/>
    </dbReference>
    <dbReference type="PDB" id="4UQV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-429"/>
    </dbReference>
    <dbReference type="PDBsum" id="4BHD"/>
    <dbReference type="PDBsum" id="4UQV"/>
    <dbReference type="AlphaFoldDB" id="Q58992"/>
    <dbReference type="SMR" id="Q58992"/>
    <dbReference type="STRING" id="243232.MJ_1597"/>
    <dbReference type="EnsemblBacteria" id="AAB99615">
        <property type="protein sequence ID" value="AAB99615"/>
        <property type="gene ID" value="MJ_1597"/>
    </dbReference>
    <dbReference type="GeneID" id="1452505"/>
    <dbReference type="KEGG" id="mja:MJ_1597"/>
    <dbReference type="eggNOG" id="arCOG00070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022477_2_1_2"/>
    <dbReference type="InParanoid" id="Q58992"/>
    <dbReference type="OMA" id="SHPAGLI"/>
    <dbReference type="OrthoDB" id="22518at2157"/>
    <dbReference type="PhylomeDB" id="Q58992"/>
    <dbReference type="BRENDA" id="2.1.2.1">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00288">
        <property type="reaction ID" value="UER01023"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004372">
        <property type="term" value="F:glycine hydroxymethyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008732">
        <property type="term" value="F:L-allo-threonine aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070905">
        <property type="term" value="F:serine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046655">
        <property type="term" value="P:folic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019264">
        <property type="term" value="P:glycine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046653">
        <property type="term" value="P:tetrahydrofolate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00378">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00051">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001085">
        <property type="entry name" value="Ser_HO-MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019798">
        <property type="entry name" value="Ser_HO-MeTrfase_PLP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039429">
        <property type="entry name" value="SHMT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11680">
        <property type="entry name" value="PTHR11680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00464">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000412">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00096">
        <property type="entry name" value="SHMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113713" description="Serine hydroxymethyltransferase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>(6S)-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
        </ligand>
    </feature>
    <feature type="site" description="Plays an important role in substrate specificity" evidence="1">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="3"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
    </feature>
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<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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<entry dataset="Swiss-Prot" created="2001-08-29" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q59037</accession>
    <name>SMC_METJA</name>
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            <fullName evidence="1">Chromosome partition protein Smc</fullName>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Required for chromosome condensation and partitioning.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains large globular domains required for ATP hydrolysis at each terminus and a third globular domain forming a flexible SMC hinge near the middle of the molecule. These domains are separated by coiled-coil structures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64505">
        <property type="entry name" value="A64505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871167.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59037"/>
    <dbReference type="SMR" id="Q59037"/>
    <dbReference type="STRING" id="243232.MJ_1643"/>
    <dbReference type="EnsemblBacteria" id="AAB99663">
        <property type="protein sequence ID" value="AAB99663"/>
        <property type="gene ID" value="MJ_1643"/>
    </dbReference>
    <dbReference type="GeneID" id="1452552"/>
    <dbReference type="KEGG" id="mja:MJ_1643"/>
    <dbReference type="eggNOG" id="arCOG00371">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001042_2_2_2"/>
    <dbReference type="InParanoid" id="Q59037"/>
    <dbReference type="OMA" id="HNKIAME"/>
    <dbReference type="OrthoDB" id="1023at2157"/>
    <dbReference type="PhylomeDB" id="Q59037"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030261">
        <property type="term" value="P:chromosome condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007062">
        <property type="term" value="P:sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01894">
        <property type="entry name" value="Smc_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003395">
        <property type="entry name" value="RecF/RecN/SMC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024704">
        <property type="entry name" value="SMC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010935">
        <property type="entry name" value="SMC_hinge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036277">
        <property type="entry name" value="SMC_hinge_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011890">
        <property type="entry name" value="SMC_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06470">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02463">
        <property type="entry name" value="SMC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005719">
        <property type="entry name" value="SMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00968">
        <property type="entry name" value="SMC_hinge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75553">
        <property type="entry name" value="SSF75553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02169">
        <property type="entry name" value="SMC_prok_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02168">
        <property type="entry name" value="SMC_prok_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119031" description="Chromosome partition protein Smc">
        <location>
            <begin position="1"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="domain" description="SMC hinge">
        <location>
            <begin position="523"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="166"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="676"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01894"/>
        </source>
    </evidence>
    <sequence length="1169" mass="136635" checksum="B63CCE34E4C03F36" modified="1998-06-01" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q59041</accession>
    <name>HMVA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>DNA-binding protein HmvA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hmvA</name>
        <name type="ordered locus">MJ1647</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Extremophiles" volume="4" first="43" last="51">
            <title>MJ1647, an open reading frame in the genome of the hyperthermophile Methanococcus jannaschii, encodes a very thermostable archaeal histone with a C-terminal extension.</title>
            <authorList>
                <person name="Li W.-T."/>
                <person name="Sandman K."/>
                <person name="Pereira S.L."/>
                <person name="Reeve J.N."/>
            </authorList>
            <dbReference type="PubMed" id="10741836"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer (PubMed:10741836). Dimers then assemble into higher oligomers, with the DNA wrapped around the protein core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64505">
        <property type="entry name" value="E64505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871171.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59041"/>
    <dbReference type="SMR" id="Q59041"/>
    <dbReference type="STRING" id="243232.MJ_1647"/>
    <dbReference type="EnsemblBacteria" id="AAB99668">
        <property type="protein sequence ID" value="AAB99668"/>
        <property type="gene ID" value="MJ_1647"/>
    </dbReference>
    <dbReference type="GeneID" id="1452556"/>
    <dbReference type="KEGG" id="mja:MJ_1647"/>
    <dbReference type="eggNOG" id="arCOG02145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340228_0_0_2"/>
    <dbReference type="OMA" id="MKENTDM"/>
    <dbReference type="OrthoDB" id="110775at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155005" description="DNA-binding protein HmvA">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="52"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P19267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10741836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="96" mass="11201" checksum="D64F2C5841855C42" modified="1996-11-01" version="1">MLPKATVKRIMKQHTDFNISAEAVDELCNMLEEIIKITTEVAEQNARKEGRKTIKARDIKQCDDERLKRKIMELSERTDKMPILIKEMLNVITSEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2022-08-03" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q59046</accession>
    <name>TOP1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA topoisomerase 1</fullName>
            <ecNumber evidence="1">5.6.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA topoisomerase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Omega-protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Relaxing enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Swivelase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Untwisting enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">topA</name>
        <name type="ordered locus">MJ1652</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.</text>
            <dbReference type="EC" id="5.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type IA topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64506">
        <property type="entry name" value="B64506"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59046"/>
    <dbReference type="SMR" id="Q59046"/>
    <dbReference type="STRING" id="243232.MJ_1652"/>
    <dbReference type="EnsemblBacteria" id="AAB99673">
        <property type="protein sequence ID" value="AAB99673"/>
        <property type="gene ID" value="MJ_1652"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1652"/>
    <dbReference type="eggNOG" id="arCOG01527">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002929_5_2_2"/>
    <dbReference type="InParanoid" id="Q59046"/>
    <dbReference type="OMA" id="PECKYTR"/>
    <dbReference type="PhylomeDB" id="Q59046"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003917">
        <property type="term" value="F:DNA topoisomerase type I (single strand cut, ATP-independent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03362">
        <property type="entry name" value="TOPRIM_TopoIA_TopoIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00952">
        <property type="entry name" value="Topoisom_1_prok"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000380">
        <property type="entry name" value="Topo_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023406">
        <property type="entry name" value="Topo_IA_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013825">
        <property type="entry name" value="Topo_IA_cen_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005739">
        <property type="entry name" value="TopoI_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028612">
        <property type="entry name" value="Topoisom_1_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034144">
        <property type="entry name" value="TOPRIM_TopoIII"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11390">
        <property type="entry name" value="PTHR11390"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="SSF56712"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01057">
        <property type="entry name" value="topA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00396">
        <property type="entry name" value="TOPOISOMERASE_I_PROK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000145178" description="DNA topoisomerase 1">
        <location>
            <begin position="1"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="6"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type 1">
        <location>
            <begin position="600"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type 2">
        <location>
            <begin position="680"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type 3">
        <location>
            <begin position="721"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="196"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="1">
        <location>
            <position position="315"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00952"/>
        </source>
    </evidence>
    <sequence length="761" mass="87833" checksum="2F9C95753E202D82" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q59064</accession>
    <name>CSM2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system Cms protein Csm2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR type III A-associated protein Csm2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">csm2</name>
        <name type="ordered locus">MJ1670</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities.</text>
    </comment>
    <comment type="function">
        <text evidence="1">This subunit may be involved in monitoring complementarity of crRNA and target RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the Csm effector complex that includes Cas10, Csm2, Csm3, Csm4 and Csm5.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Encoded in a type III-A CRISPR locus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm2 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64508">
        <property type="entry name" value="D64508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871194.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59064"/>
    <dbReference type="SMR" id="Q59064"/>
    <dbReference type="STRING" id="243232.MJ_1670"/>
    <dbReference type="EnsemblBacteria" id="AAB99693">
        <property type="protein sequence ID" value="AAB99693"/>
        <property type="gene ID" value="MJ_1670"/>
    </dbReference>
    <dbReference type="GeneID" id="1452579"/>
    <dbReference type="KEGG" id="mja:MJ_1670"/>
    <dbReference type="eggNOG" id="arCOG06487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131491_2_0_2"/>
    <dbReference type="InParanoid" id="Q59064"/>
    <dbReference type="OMA" id="KPKLAYN"/>
    <dbReference type="OrthoDB" id="127804at2157"/>
    <dbReference type="PhylomeDB" id="Q59064"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09647">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010149">
        <property type="entry name" value="CRISPR-assoc_prot_Csm2_III-A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03750">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01870">
        <property type="entry name" value="cas_TM1810_Csm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000107468" description="CRISPR system Cms protein Csm2">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0A7HIX1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="133" mass="15670" checksum="EF803498557B6898" modified="1996-11-01" version="1">MSKIGKCILNPNEINLILNINSQNANEIIDIAENLAKEFEQIPATKMRDFYDYVLRIDEKNENWYKELVLLKPKLAYNYGKETNRRKKEALEKLAGTFSEIIDKIDNDLNKFKNFKTFFEALVAYHKIYAKSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q59068</accession>
    <name>Y1674_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated protein MJ1674</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1674</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Encoded in a type III-A CRISPR locus.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64508">
        <property type="entry name" value="H64508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871198.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59068"/>
    <dbReference type="SMR" id="Q59068"/>
    <dbReference type="STRING" id="243232.MJ_1674"/>
    <dbReference type="EnsemblBacteria" id="AAB99696">
        <property type="protein sequence ID" value="AAB99696"/>
        <property type="gene ID" value="MJ_1674"/>
    </dbReference>
    <dbReference type="GeneID" id="1452583"/>
    <dbReference type="KEGG" id="mja:MJ_1674"/>
    <dbReference type="eggNOG" id="arCOG07641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025124_2_0_2"/>
    <dbReference type="InParanoid" id="Q59068"/>
    <dbReference type="OMA" id="IFEISEW"/>
    <dbReference type="OrthoDB" id="20895at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013383">
        <property type="entry name" value="CRISPR-assoc_prot_DxTHG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011742">
        <property type="entry name" value="CRISPR-assoc_prot_TM1812"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Cas_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02221">
        <property type="entry name" value="cas_TM1812"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02549">
        <property type="entry name" value="CRISPR_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107472" description="CRISPR-associated protein MJ1674">
        <location>
            <begin position="1"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="634" mass="74782" checksum="E91877838C81AB7F" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q59076</accession>
    <name>HTPX_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protease HtpX homolog</fullName>
            <ecNumber evidence="1">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">htpX</name>
        <name type="ordered locus">MJ1682</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M48B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64509">
        <property type="entry name" value="H64509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871206.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59076"/>
    <dbReference type="SMR" id="Q59076"/>
    <dbReference type="STRING" id="243232.MJ_1682"/>
    <dbReference type="EnsemblBacteria" id="AAB99703">
        <property type="protein sequence ID" value="AAB99703"/>
        <property type="gene ID" value="MJ_1682"/>
    </dbReference>
    <dbReference type="GeneID" id="1452591"/>
    <dbReference type="KEGG" id="mja:MJ_1682"/>
    <dbReference type="eggNOG" id="arCOG01331">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042266_3_0_2"/>
    <dbReference type="InParanoid" id="Q59076"/>
    <dbReference type="OMA" id="AVCCTEG"/>
    <dbReference type="OrthoDB" id="62484at2157"/>
    <dbReference type="PhylomeDB" id="Q59076"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138917" description="Protease HtpX homolog">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <sequence length="284" mass="31972" checksum="86B51795599F560A" modified="1996-11-01" version="1">MINQIKTYLLMALLVGLIYAICMMLHIHPLIAIILALIPNVIAYYMSDKLVLMSYNARILEEHEMPWLHQMVERVARKAGLPKPKVAIVPTETPNAFATGRNPENAVVAVTEGILKLLSPEELEGVIGHEISHIKHRDILISTIVATLAGAIVMIAEWMLYWGGIFFVSEEEESNPLELIGTILLLILAPIAATIIQFAISRQREFYADEEGAKLTHPLWLANALAKLERGVELYPLERGNPATAHMFIINPFRKDFIAKLFSTHPPTEERIERLLEMCKRIGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q60175</accession>
    <name>SECY_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">secY</name>
        <name type="ordered locus">MJ0478</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Nature" volume="427" first="36" last="44">
            <title>X-ray structure of a protein-conducting channel.</title>
            <authorList>
                <person name="Van den Berg B."/>
                <person name="Clemons W.M. Jr."/>
                <person name="Collinson I."/>
                <person name="Modis Y."/>
                <person name="Hartmann E."/>
                <person name="Harrison S.C."/>
                <person name="Rapoport T.A."/>
            </authorList>
            <dbReference type="PubMed" id="14661030"/>
            <dbReference type="DOI" id="10.1038/nature02218"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 1-423 IN COMPLEX WITH SECE AND SECG</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2007" name="Mol. Cell" volume="26" first="511" last="521">
            <title>The plug domain of the SecY protein stabilizes the closed state of the translocation channel and maintains a membrane seal.</title>
            <authorList>
                <person name="Li W."/>
                <person name="Schulman S."/>
                <person name="Boyd D."/>
                <person name="Erlandson K."/>
                <person name="Beckwith J."/>
                <person name="Rapoport T.A."/>
            </authorList>
            <dbReference type="PubMed" id="17531810"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2007.05.002"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF PLUG DELETION MUTANTS</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Mol. Cell" volume="28" first="1083" last="1092">
            <title>Ribosome binding of a single copy of the SecY complex: implications for protein translocation.</title>
            <authorList>
                <person name="Menetret J.F."/>
                <person name="Schaletzky J."/>
                <person name="Clemons W.M. Jr."/>
                <person name="Osborne A.R."/>
                <person name="Skanland S.S."/>
                <person name="Denison C."/>
                <person name="Gygi S.P."/>
                <person name="Kirkpatrick D.S."/>
                <person name="Park E."/>
                <person name="Ludtke S.J."/>
                <person name="Rapoport T.A."/>
                <person name="Akey C.W."/>
            </authorList>
            <dbReference type="PubMed" id="18158904"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2007.10.034"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (9.6 ANGSTROMS) OF 2-433 DOCKED ONTO E.COLI RIBOSOMES</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2008" name="Structure" volume="16" first="1126" last="1137">
            <title>Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome.</title>
            <authorList>
                <person name="Menetret J.F."/>
                <person name="Hegde R.S."/>
                <person name="Aguiar M."/>
                <person name="Gygi S.P."/>
                <person name="Park E."/>
                <person name="Rapoport T.A."/>
                <person name="Akey C.W."/>
            </authorList>
            <dbReference type="PubMed" id="18611385"/>
            <dbReference type="DOI" id="10.1016/j.str.2008.05.003"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (8.7 ANGSTROMS) OF 2-433 DOCKED ONTO DOG RIBOSOMES</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2009" name="Structure" volume="17" first="1453" last="1464">
            <title>Regulation of the protein-conducting channel by a bound ribosome.</title>
            <authorList>
                <person name="Gumbart J."/>
                <person name="Trabuco L.G."/>
                <person name="Schreiner E."/>
                <person name="Villa E."/>
                <person name="Schulten K."/>
            </authorList>
            <dbReference type="PubMed" id="19913480"/>
            <dbReference type="DOI" id="10.1016/j.str.2009.09.010"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY OF 2-433 DOCKED ONTO E.COLI RIBOSOMES</scope>
    </reference>
    <comment type="function">
        <text>The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cell membrane</location>
            <topology>Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869979.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1RH5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="A=1-436"/>
    </dbReference>
    <dbReference type="PDB" id="1RHZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="A=1-436"/>
    </dbReference>
    <dbReference type="PDB" id="2YXQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
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</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q60257</accession>
    <name>Y3536_METJA</name>
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        <recommendedName>
            <fullName>UPF0310 protein MJECL36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL36</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
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        <name>large ECE</name>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
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        <text evidence="1">Belongs to the UPF0310 family.</text>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000059636" description="UPF0310 protein MJECL36">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="21"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="26"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="29"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="40"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="105"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="129"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="135"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2P5D"/>
        </source>
    </evidence>
    <sequence length="144" mass="17183" checksum="11B4604B5D323070" modified="2004-05-10" version="2">MAYWLCITNEDNWKVIKEKKIWGVAERYKNTINKVKVGDKLIIYEIQRSGKDYKPPYIRGVYEVVSEVYKDSSKIFKPTPRNPNEKFPYRVKLKEIKVFEPPINFKELIPKLKFITNKKRWSGHLMGKAMREIPEEDYKLIVGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q60265</accession>
    <name>Y3531_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative antitoxin MJECL31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL31</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possibly the antitoxin component of a type II toxin-antitoxin (TA) system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0165 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAC37074" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37074.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64513">
        <property type="entry name" value="F64513"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496999.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60265"/>
    <dbReference type="SMR" id="Q60265"/>
    <dbReference type="PRIDE" id="Q60265"/>
    <dbReference type="EnsemblBacteria" id="AAC37074">
        <property type="protein sequence ID" value="AAC37074"/>
        <property type="gene ID" value="MJ_ECL31"/>
    </dbReference>
    <dbReference type="GeneID" id="1450787"/>
    <dbReference type="KEGG" id="mja:MJ_ECL31"/>
    <dbReference type="eggNOG" id="arCOG07152">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200885_0_1_2"/>
    <dbReference type="InParanoid" id="Q60265"/>
    <dbReference type="OMA" id="CKDIDYK"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008203">
        <property type="entry name" value="AF2212-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024069">
        <property type="entry name" value="AF2212-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01954">
        <property type="entry name" value="DUF104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000156859" description="Putative antitoxin MJECL31">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="65" mass="7737" checksum="DFE63A96F3FCF801" modified="2001-06-20" version="2">MSEIIEVIYEDGVLKPLKPLKIKGKKRLKIKIVNDDVEEFLKSMIIKKCKDIDYKKLKEAYYESF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q60315</accession>
    <name>Y004_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0004</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0004</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64300">
        <property type="entry name" value="D64300"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869497.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60315"/>
    <dbReference type="SMR" id="Q60315"/>
    <dbReference type="STRING" id="243232.MJ_0004"/>
    <dbReference type="EnsemblBacteria" id="AAB97985">
        <property type="protein sequence ID" value="AAB97985"/>
        <property type="gene ID" value="MJ_0004"/>
    </dbReference>
    <dbReference type="GeneID" id="1450843"/>
    <dbReference type="KEGG" id="mja:MJ_0004"/>
    <dbReference type="eggNOG" id="arCOG02678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066597_0_0_2"/>
    <dbReference type="InParanoid" id="Q60315"/>
    <dbReference type="OMA" id="GKGANYF"/>
    <dbReference type="OrthoDB" id="72761at2157"/>
    <dbReference type="PhylomeDB" id="Q60315"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106647" description="Uncharacterized protein MJ0004">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="243" mass="26897" checksum="288C4F4190575E53" modified="1997-11-01" version="1">MILGIDVGSTTTKMVLMEDSKIIWYKIEDIGVVIEEDILLKMVKEIEQKYPIDKIVATGYGRHKVSFADKIVPEVIALGKGANYFFNEADGVIDIGGQDTKVLKIDKNGKVVDFILSDKCAAGTGKFLEKALDILKIDKNEINKYKSDNIAKISSMCAVFAESEIISLLSKKVPKEGILMGVYESIINRVIPMTNRLKIQNIVFSGGVAKNKVLVEMFEKKLNKKLLIPKEPQIVCCVGAILV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q60318</accession>
    <name>Y007_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative dehydratase subunit MJ0007</fullName>
            <ecNumber>4.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0007</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FldB/FldC dehydratase alpha/beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="4.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64300">
        <property type="entry name" value="G64300"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60318"/>
    <dbReference type="SMR" id="Q60318"/>
    <dbReference type="STRING" id="243232.MJ_0007"/>
    <dbReference type="EnsemblBacteria" id="AAB97988">
        <property type="protein sequence ID" value="AAB97988"/>
        <property type="gene ID" value="MJ_0007"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0007"/>
    <dbReference type="eggNOG" id="arCOG04464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053697_1_1_2"/>
    <dbReference type="InParanoid" id="Q60318"/>
    <dbReference type="OMA" id="CPYMYFS"/>
    <dbReference type="PhylomeDB" id="Q60318"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010327">
        <property type="entry name" value="FldB/FldC_alpha/beta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06050">
        <property type="entry name" value="HGD-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106648" description="Putative dehydratase subunit MJ0007">
        <location>
            <begin position="1"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="373" mass="42984" checksum="BA370B62EB70202D" modified="1997-11-01" version="1">MMKLKAIEKLMQKFASRKEQLYKQKEEGRKVFGMFCAYVPIEIILAANAIPVGLCGGKNDTIPIAEEDLPRNLCPLIKSSYGFKKAKTCPYFEASDIVIGETTCEGKKKMFELMERLVPMHIMHLPHMKDEDSLKIWIKEVEKLKELVEKETGNKITEEKLKETVDKVNKVRELFYKLYELRKNKPAPIKGLDVLKLFQFAYLLDIDDTIGILEDLIEELEERVKKGEGYEGKRILITGCPMVAGNNKIVEIIEEVGGVVVGEESCTGTRFFENFVEGYSVEDIAKRYFKIPCACRFKNDERVENIKRLVKELDVDGVVYYTLQYCHTFNIEGAKVEEALKEEGIPIIRIETDYSESDREQLKTRLEAFIEMI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q60342</accession>
    <name>CBID_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiD</name>
        <name type="ordered locus">MJ0022</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64302">
        <property type="entry name" value="F64302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869514.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60342"/>
    <dbReference type="SMR" id="Q60342"/>
    <dbReference type="STRING" id="243232.MJ_0022"/>
    <dbReference type="EnsemblBacteria" id="AAB98004">
        <property type="protein sequence ID" value="AAB98004"/>
        <property type="gene ID" value="MJ_0022"/>
    </dbReference>
    <dbReference type="GeneID" id="1450860"/>
    <dbReference type="KEGG" id="mja:MJ_0022"/>
    <dbReference type="eggNOG" id="arCOG04383">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041273_1_0_2"/>
    <dbReference type="InParanoid" id="Q60342"/>
    <dbReference type="OMA" id="YHGKLIK"/>
    <dbReference type="OrthoDB" id="57676at2157"/>
    <dbReference type="PhylomeDB" id="Q60342"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046140">
        <property type="term" value="P:corrin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="PTHR35863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="SSF111342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141692" description="Cobalt-precorrin-5B C(1)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <sequence length="362" mass="39951" checksum="6AA2947C871906B7" modified="1997-11-01" version="1">MIYDFRKKSKFGYTTGSCAAAGAYSALYYLKFGKKLSYVEIENLNGDKLIIPIEKIEKCGNKAKAVVIKDAGGDIDITNGIEIITEVELKKGKKDVIIKGGEGVGIVTKNGLQVKKGEPAINPKPREMIRNNLLKLLNDDEVVEVTISIPKGKELAKKTLNPKLGIVGGLSILGTTGIVRPMSNEAYMNSLAPQIDVALANGYKRLIFVPGNIGTKYAKQLLNANDDEIIEVSNFWGFMLDKAKEKGVEEILIFGHAGKIIKLAGGIYNTHSKVADCRNEILAAYSSLFIDDKEAIKKILYSNTTEEVIKILEEKGVLNDVFNFIAKRVVERLSERWKGIKFSCIIINMKGEVLGSYIWNKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q60343</accession>
    <name>TRM4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>tRNA (cytosine(48)-C(5))-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>aTrm4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA (cytosine-5-)-methyltransferase Trm4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">trm4</name>
        <name type="ordered locus">MJ0026</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="J. Mol. Biol." volume="401" first="323" last="333">
            <title>Crystal structure of Methanocaldococcus jannaschii Trm4 complexed with sinefungin.</title>
            <authorList>
                <person name="Kuratani M."/>
                <person name="Hirano M."/>
                <person name="Goto-Ito S."/>
                <person name="Itoh Y."/>
                <person name="Hikida Y."/>
                <person name="Nishimoto M."/>
                <person name="Sekine S."/>
                <person name="Bessho Y."/>
                <person name="Ito T."/>
                <person name="Grosjean H."/>
                <person name="Yokoyama S."/>
            </authorList>
            <dbReference type="PubMed" id="20600111"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2010.06.046"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.27 ANGSTROMS) IN COMPLEX WITH SINEFUNGIN</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes AdoMet-dependent formation of m5C in tRNA. Cytidine residue at either position 40 or position 48 is likely to be methylated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>cytidine(48) in tRNA precursor + S-adenosyl-L-methionine = 5-methylcytidine(48) in tRNA precursor + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:54136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13806"/>
            <dbReference type="Rhea" id="RHEA-COMP:13807"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>cytidine(40) in tRNA precursor + S-adenosyl-L-methionine = 5-methylcytidine(40) in tRNA precursor + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42944"/>
            <dbReference type="Rhea" id="RHEA-COMP:10292"/>
            <dbReference type="Rhea" id="RHEA-COMP:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74483"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64303">
        <property type="entry name" value="B64303"/>
    </dbReference>
    <dbReference type="PDB" id="3A4T">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=1-274"/>
    </dbReference>
    <dbReference type="PDB" id="3AJD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.27 A"/>
        <property type="chains" value="A=1-274"/>
    </dbReference>
    <dbReference type="PDBsum" id="3A4T"/>
    <dbReference type="PDBsum" id="3AJD"/>
    <dbReference type="AlphaFoldDB" id="Q60343"/>
    <dbReference type="SMR" id="Q60343"/>
    <dbReference type="STRING" id="243232.MJ_0026"/>
    <dbReference type="PRIDE" id="Q60343"/>
    <dbReference type="EnsemblBacteria" id="AAB98007">
        <property type="protein sequence ID" value="AAB98007"/>
        <property type="gene ID" value="MJ_0026"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0026"/>
    <dbReference type="eggNOG" id="arCOG00973">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005316_7_0_2"/>
    <dbReference type="InParanoid" id="Q60343"/>
    <dbReference type="OMA" id="EPEENEF"/>
    <dbReference type="PhylomeDB" id="Q60343"/>
    <dbReference type="EvolutionaryTrace" id="Q60343"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="Fmu/NOL1/Nop2p_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB/NOP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="PTHR22807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000211821" description="tRNA (cytosine(48)-C(5))-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
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    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="212"/>
        </location>
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    <feature type="binding site">
        <location>
            <begin position="91"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="11"/>
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        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="17"/>
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        </location>
    </feature>
    <feature type="helix" evidence="6">
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    <feature type="strand" evidence="6">
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    <feature type="strand" evidence="6">
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    <feature type="strand" evidence="6">
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    <feature type="helix" evidence="6">
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    <feature type="helix" evidence="6">
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    <feature type="strand" evidence="6">
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        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="157"/>
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    <feature type="helix" evidence="5">
        <location>
            <begin position="167"/>
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    <feature type="helix" evidence="6">
        <location>
            <begin position="180"/>
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    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="190"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="216"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="231"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="248"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="257"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="266"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="20600111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="20600111"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3A4T"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3AJD"/>
        </source>
    </evidence>
    <sequence length="274" mass="31490" checksum="F7396827523369CF" modified="1997-11-01" version="1">MMIVYKGEKMQFIRVNTLKINPEVLKKRLENKGVVLEKTFLDYAFEVKKSPFSIGSTPEYLFGYYMPQSISSMIPPIVLNPREDDFILDMCAAPGGKTTHLAQLMKNKGTIVAVEISKTRTKALKSNINRMGVLNTIIINADMRKYKDYLLKNEIFFDKILLDAPCSGNIIKDKNRNVSEEDIKYCSLRQKELIDIGIDLLKKDGELVYSTCSMEVEENEEVIKYILQKRNDVELIIIKANEFKGINIKEGYIKGTLRVFPPNEPFFIAKLRKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q60390</accession>
    <name>MCRY_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase II subunit beta</fullName>
            <shortName>MCR II beta</shortName>
            <ecNumber evidence="1">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme-B sulfoethylthiotransferase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mrtB</name>
        <name type="ordered locus">MJ0081</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="1">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-coenzyme M reductase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869573.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60390"/>
    <dbReference type="SMR" id="Q60390"/>
    <dbReference type="STRING" id="243232.MJ_0081"/>
    <dbReference type="EnsemblBacteria" id="AAB98061">
        <property type="protein sequence ID" value="AAB98061"/>
        <property type="gene ID" value="MJ_0081"/>
    </dbReference>
    <dbReference type="GeneID" id="1450920"/>
    <dbReference type="KEGG" id="mja:MJ_0081"/>
    <dbReference type="eggNOG" id="arCOG04860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617682_0_0_2"/>
    <dbReference type="InParanoid" id="Q60390"/>
    <dbReference type="OMA" id="WALWNAY"/>
    <dbReference type="OrthoDB" id="25508at2157"/>
    <dbReference type="PhylomeDB" id="Q60390"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003179">
        <property type="entry name" value="Me_CoM_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022679">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022680">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02241">
        <property type="entry name" value="MCR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02783">
        <property type="entry name" value="MCR_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000263">
        <property type="entry name" value="Meth_CoM_rd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03257">
        <property type="entry name" value="met_CoM_red_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147466" description="Methyl-coenzyme M reductase II subunit beta">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P11560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="447" mass="47770" checksum="B9CAE1066BE977BC" modified="1998-12-15" version="2">MLHYEDRIDLYDERGKLLEENVPLEAISPLKNPTIEKIVNDIKRSVAINLAGIENALKTGAVGGKACFCPGRELDLPIVENAEIIAEKIKRMVQIEEDDDTVVKLINGGKQLLLQLPSKRLRVAADYTVSALIGGGATVQAIVDAFDVDMFDAPVVKTAVMGRYPQTVDFHGANIATLLGPPVLLEGLGYGLRNIMANHIVAVTRKKTLNAVALASILEQTAMFETGDALGAFERLHLLGLAFQGLNANNLTYELVKENGKDGTVGTVVASVVERALEDGVIRPLKTMPSGFTVYEPVDWALWNAYAASGLVAAVIVNVGAARAAQGVASTVLYYNDILEYETGLPSVDFGRAEGTAVGFSFFSHSIYGGGGPGTFHGNHVVTRHSKGFAIPCAAAAMCLDAGTQMFSVERTSALVGTVYSAIDHLREPLKYVAEGAVEVKEKEKVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P54016</accession>
    <name>RL23_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl23</name>
        <name type="ordered locus">MJ0178</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64322">
        <property type="entry name" value="C64322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869673.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54016"/>
    <dbReference type="SMR" id="P54016"/>
    <dbReference type="STRING" id="243232.MJ_0178"/>
    <dbReference type="EnsemblBacteria" id="AAB98163">
        <property type="protein sequence ID" value="AAB98163"/>
        <property type="gene ID" value="MJ_0178"/>
    </dbReference>
    <dbReference type="GeneID" id="1451025"/>
    <dbReference type="KEGG" id="mja:MJ_0178"/>
    <dbReference type="eggNOG" id="arCOG04072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037562_4_2_2"/>
    <dbReference type="InParanoid" id="P54016"/>
    <dbReference type="OMA" id="MDPYKVI"/>
    <dbReference type="OrthoDB" id="113971at2157"/>
    <dbReference type="PhylomeDB" id="P54016"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_L23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_L23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_L23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_L23/L25_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_L25/23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="PTHR11620"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129437" description="50S ribosomal protein L23">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <sequence length="86" mass="9856" checksum="DE53C1A1A361697B" modified="1996-10-01" version="1">MDAFDVIKAPVVTEKTVRMIEEENKLVFYVDRRATKQDIKRAMKELFDVEVEKVNTLITPKGEKKAYVKLKEGYDASKIAASLGIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>P54019</accession>
    <name>RS13_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps13</name>
        <name type="ordered locus">MJ0189</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869684.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54019"/>
    <dbReference type="SMR" id="P54019"/>
    <dbReference type="STRING" id="243232.MJ_0189"/>
    <dbReference type="EnsemblBacteria" id="AAB98169">
        <property type="protein sequence ID" value="AAB98169"/>
        <property type="gene ID" value="MJ_0189"/>
    </dbReference>
    <dbReference type="GeneID" id="1451037"/>
    <dbReference type="KEGG" id="mja:MJ_0189"/>
    <dbReference type="eggNOG" id="arCOG01722">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103849_0_0_2"/>
    <dbReference type="InParanoid" id="P54019"/>
    <dbReference type="OMA" id="IRAYRGI"/>
    <dbReference type="OrthoDB" id="84219at2157"/>
    <dbReference type="PhylomeDB" id="P54019"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_S13_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="30s_Rbsml_prot_S13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_S13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_S13_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_S13_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000132181" description="30S ribosomal protein S13">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="150" mass="17380" checksum="DD4014968FD075CB" modified="1998-07-15" version="2">MQNSEFKYLIRVSRTDLDGNKKLIMALQDIYGVGEAMARAIVRVAKLDPNKLAGYLTEEEVKKIEEVLADPAKFGIPSWMFNRRKDYVTGEDKHVIESDLMIIKQEDINRLKRIRCYRGIRHELGLPCRGQRTKSTFRRGPTVGVSRRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>P54041</accession>
    <name>RS8_METJA</name>
    <protein>
        <recommendedName>
            <fullName>30S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rps8</name>
        <name type="ordered locus">MJ0470</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="J. Mol. Biol." volume="311" first="311" last="324">
            <title>Detailed analysis of RNA-protein interactions within the ribosomal protein S8-rRNA complex from the archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Tishchenko S."/>
                <person name="Nikulin A."/>
                <person name="Fomenkova N."/>
                <person name="Nevskaya N."/>
                <person name="Nikonov O."/>
                <person name="Dumas P."/>
                <person name="Moine H."/>
                <person name="Ehresmann B."/>
                <person name="Ehresmann C."/>
                <person name="Piendl W."/>
                <person name="Lamzin V."/>
                <person name="Garber M.B."/>
                <person name="Nikonov S."/>
            </authorList>
            <dbReference type="PubMed" id="11478863"/>
            <dbReference type="DOI" id="10.1006/jmbi.2001.4877"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text>Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64358">
        <property type="entry name" value="F64358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869971.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1I6U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B=1-130"/>
    </dbReference>
    <dbReference type="PDBsum" id="1I6U"/>
    <dbReference type="AlphaFoldDB" id="P54041"/>
    <dbReference type="SMR" id="P54041"/>
    <dbReference type="STRING" id="243232.MJ_0470"/>
    <dbReference type="EnsemblBacteria" id="AAB98459">
        <property type="protein sequence ID" value="AAB98459"/>
        <property type="gene ID" value="MJ_0470"/>
    </dbReference>
    <dbReference type="GeneID" id="8805616"/>
    <dbReference type="KEGG" id="mja:MJ_0470"/>
    <dbReference type="eggNOG" id="arCOG04091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098428_1_1_2"/>
    <dbReference type="InParanoid" id="P54041"/>
    <dbReference type="OMA" id="LPAKNFG"/>
    <dbReference type="OrthoDB" id="91154at2157"/>
    <dbReference type="PhylomeDB" id="P54041"/>
    <dbReference type="EvolutionaryTrace" id="P54041"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_S8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_S8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_S8_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="PTHR11758"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
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        <property type="match status" value="1"/>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
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                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
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    <proteinExistence type="predicted"/>
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        </location>
    </feature>
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</entry>
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    <accession>Q57566</accession>
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            <fullName evidence="1">Flavin prenyltransferase UbiX</fullName>
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        <name evidence="1" type="primary">ubiX</name>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
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                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
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                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dimethylallyl phosphate + FMNH2 = phosphate + prenyl-FMNH2</text>
            <dbReference type="Rhea" id="RHEA:37743"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:87467"/>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
            <dbReference type="EC" id="2.5.1.129"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiX/PAD1 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.129" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64312">
        <property type="entry name" value="F64312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869594.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6M8V">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.22 A"/>
        <property type="chains" value="A=1-184"/>
    </dbReference>
    <dbReference type="PDBsum" id="6M8V"/>
    <dbReference type="AlphaFoldDB" id="Q57566"/>
    <dbReference type="SMR" id="Q57566"/>
    <dbReference type="STRING" id="243232.MJ_0102"/>
    <dbReference type="EnsemblBacteria" id="AAB98082">
        <property type="protein sequence ID" value="AAB98082"/>
        <property type="gene ID" value="MJ_0102"/>
    </dbReference>
    <dbReference type="GeneID" id="1450941"/>
    <dbReference type="KEGG" id="mja:MJ_0102"/>
    <dbReference type="eggNOG" id="arCOG01703">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074522_0_0_2"/>
    <dbReference type="InParanoid" id="Q57566"/>
    <dbReference type="OMA" id="GATHIQD"/>
    <dbReference type="OrthoDB" id="112962at2157"/>
    <dbReference type="PhylomeDB" id="Q57566"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106141">
        <property type="term" value="F:flavin prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01984">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43374">
        <property type="entry name" value="PTHR43374"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0637">Prenyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134978" description="Flavin prenyltransferase UbiX">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>dimethylallyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:88052"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="2"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="23"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="27"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="56"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="77"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="86"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="100"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="114"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="126"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01984"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="6M8V"/>
        </source>
    </evidence>
    <sequence length="184" mass="20643" checksum="F3EDBFE5B48A82C5" modified="1997-11-01" version="1">MKIIVCITGASGVIYAKRLLEVLKDRAEVNLIISNSAKKIIKEELDIDWKEIKKLATDYYENDDFFSPLASGSNKFDAVVVVPCSMKTLSAIANGYSANLIVRVCDIALKERRKLIIMPREMPFNSIHLENMLKLSNLGAIVMPPIPAFYNKPKNVNDIINFVVGRVLDILGIDNSLFKRWGTV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q57568</accession>
    <name>Y104_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-dependent helicase MJ0104</fullName>
            <ecNumber>3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0104</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64312">
        <property type="entry name" value="H64312"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57568"/>
    <dbReference type="SMR" id="Q57568"/>
    <dbReference type="STRING" id="243232.MJ_0104"/>
    <dbReference type="EnsemblBacteria" id="AAB98084">
        <property type="protein sequence ID" value="AAB98084"/>
        <property type="gene ID" value="MJ_0104"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0104"/>
    <dbReference type="eggNOG" id="arCOG00792">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001666_8_2_2"/>
    <dbReference type="InParanoid" id="Q57568"/>
    <dbReference type="OMA" id="YANDITF"/>
    <dbReference type="PhylomeDB" id="Q57568"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="PTHR10887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00376">
        <property type="entry name" value="TIGR00376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000080730" description="Uncharacterized ATP-dependent helicase MJ0104">
        <location>
            <begin position="1"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="207"/>
            <end position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="663" mass="77573" checksum="590C64A66AB4DE4F" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q57582</accession>
    <name>MCRW_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase II operon protein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mrtD</name>
        <name type="synonym">mtrD</name>
        <name type="ordered locus">MJ0118</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">MCR is composed of three subunits: alpha, beta, and gamma. The function of protein D is not known.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64314">
        <property type="entry name" value="F64314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57582"/>
    <dbReference type="STRING" id="243232.MJ_0118"/>
    <dbReference type="EnsemblBacteria" id="AAB98099">
        <property type="protein sequence ID" value="AAB98099"/>
        <property type="gene ID" value="MJ_0118"/>
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    <dbReference type="KEGG" id="mja:MJ_0118"/>
    <dbReference type="eggNOG" id="arCOG04859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118415_0_0_2"/>
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    <dbReference type="OMA" id="NFPYGYD"/>
    <dbReference type="PhylomeDB" id="Q57582"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003901">
        <property type="entry name" value="Me_CoM_Rdtase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02505">
        <property type="entry name" value="MCR_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005636">
        <property type="entry name" value="McrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03260">
        <property type="entry name" value="met_CoM_red_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147495" description="Methyl-coenzyme M reductase II operon protein D">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <sequence length="167" mass="19007" checksum="87B67CFC6B19AF1F" modified="1997-11-01" version="1">MKNEVKFMSEVIKVVDVKIFPHRYLKAETTEKVLNEIYDLDGIVRVIVHGQPLPKTVPFGPARGLPVNHQDRKVIKVKGEEMELRVKVGEIIITVEGEKLEKIMDGIEEICKRNFPFGYDIYVGAFTKIKPTVTDYMKYGDISSIDPRLIGMVDASARLKDSVKMIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57600</accession>
    <name>PURP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="1">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purP</name>
        <name type="ordered locus">MJ0136</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Biol. Chem." volume="280" first="10881" last="10887">
            <title>A Methanocaldococcus jannaschii archaeal signature gene encodes for a 5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase: a new enzyme in purine biosynthesis.</title>
            <authorList>
                <person name="Ownby K."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="15623504"/>
            <dbReference type="DOI" id="10.1074/jbc.M413937200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="Biochemistry" volume="47" first="205" last="217">
            <title>Crystal structure and function of 5-formaminoimidazole-4-carboxamide ribonucleotide synthetase from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Zhang Y."/>
                <person name="White R.H."/>
                <person name="Ealick S.E."/>
            </authorList>
            <dbReference type="PubMed" id="18069798"/>
            <dbReference type="DOI" id="10.1021/bi701406g"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEXES WITH ATP AND SUBSTRATE AICAR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + ATP + formate = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by ADP.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 6.25.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer. Dimer of trimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphohexose mutase family.</text>
    </comment>
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    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H64316"/>
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    <dbReference type="RefSeq" id="WP_010869629.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="PDB" id="2R7K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-361"/>
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    <dbReference type="PDB" id="2R7L">
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        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-361"/>
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        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-361"/>
    </dbReference>
    <dbReference type="PDB" id="2R7N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=1-361"/>
    </dbReference>
    <dbReference type="PDBsum" id="2R7K"/>
    <dbReference type="PDBsum" id="2R7L"/>
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    <dbReference type="EnsemblBacteria" id="AAB98117">
        <property type="protein sequence ID" value="AAB98117"/>
        <property type="gene ID" value="MJ_0136"/>
    </dbReference>
    <dbReference type="GeneID" id="1450977"/>
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        <property type="organism ID" value="3260"/>
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="292"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="306"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="317"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="329"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="339"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="356"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15623504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18069798"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2R7K"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2R7N"/>
        </source>
    </evidence>
    <sequence length="361" mass="40824" checksum="0B9928DDD41D4DB6" modified="1997-11-01" version="1">MISKDEILEIFDKYNKDEITIATLGSHTSLHILKGAKLEGFSTVCITMKGRDVPYKRFKVADKFIYVDNFSDIKNEEIQEKLRELNSIVVPHGSFIAYCGLDNVENSFLVPMFGNRRILRWESERSLEGKLLREAGLRVPKKYESPEDIDGTVIVKFPGARGGRGYFIASSTEEFYKKAEDLKKRGILTDEDIANAHIEEYVVGTNFCIHYFYSPLKDEVELLGMDKRYESNIDGLVRIPAKDQLEMNINPSYVITGNIPVVIRESLLPQVFEMGDKLVAKAKELVPPGMIGPFCLQSLCNENLELVVFEMSARVDGGTNSFMNGGPYSFLYNGEPLSMGQRIAREIKMALQLDMIDKIIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q57608</accession>
    <name>Y144_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0144</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0144</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64318">
        <property type="entry name" value="A64318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869639.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57608"/>
    <dbReference type="SMR" id="Q57608"/>
    <dbReference type="STRING" id="243232.MJ_0144"/>
    <dbReference type="EnsemblBacteria" id="AAB98127">
        <property type="protein sequence ID" value="AAB98127"/>
        <property type="gene ID" value="MJ_0144"/>
    </dbReference>
    <dbReference type="GeneID" id="1450988"/>
    <dbReference type="KEGG" id="mja:MJ_0144"/>
    <dbReference type="eggNOG" id="arCOG00605">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1118194_0_0_2"/>
    <dbReference type="InParanoid" id="Q57608"/>
    <dbReference type="OMA" id="ELNCHCR"/>
    <dbReference type="OrthoDB" id="72918at2157"/>
    <dbReference type="PhylomeDB" id="Q57608"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02911">
        <property type="entry name" value="arch_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035587">
        <property type="entry name" value="DUS-like_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037347">
        <property type="entry name" value="MJ0144_FMN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005270">
        <property type="entry name" value="tRNA_dU_NifR3-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01207">
        <property type="entry name" value="Dus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00736">
        <property type="entry name" value="nifR3_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106718" description="Uncharacterized protein MJ0144">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <sequence length="258" mass="29754" checksum="F83AF4A8D7BF0836" modified="1997-11-01" version="1">MNKKIEELNKLDKKVVLAPMAGITDGDFCRKFKDLFAIVTIGGYNLDSATYKASRDIEKRGRKEFSINLEEFNSYIIEQIKKARESNALVSVNVRFVDIDEAYDKLLTIAKHADIIELNCHCRQPEITSLGIGQELMKNKNLLKEFLTKMKELNKPIFLKIRLNCIPLKELIDNLNYVRDYFDGLHVDCFYPGKPYADMDSLKILAEEFNDKIIIGNNSIDSIEKAKEMLKYSDFVSVARTILKGNVEWIKELNKENI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57611</accession>
    <name>Y147_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0147</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0147</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64318">
        <property type="entry name" value="D64318"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57611"/>
    <dbReference type="SMR" id="Q57611"/>
    <dbReference type="STRING" id="243232.MJ_0147"/>
    <dbReference type="DNASU" id="1450991"/>
    <dbReference type="EnsemblBacteria" id="AAB98139">
        <property type="protein sequence ID" value="AAB98139"/>
        <property type="gene ID" value="MJ_0147"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0147"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q57611"/>
    <dbReference type="OMA" id="FLEIMEY"/>
    <dbReference type="PhylomeDB" id="Q57611"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184667" description="Uncharacterized ATP-binding protein MJ0147">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="371" mass="43859" checksum="088E8FC7D414F8BE" modified="1997-11-01" version="1">MVIQMKFFNREKEIHEILSILEGEPNIIYFIYGPLNSGKTALIKHIIENKLSDDYKVFYINFRTYLISEKREFIEAIFTTKKDDFFEKIKDKSEVLNLITKGAKILTGIPIPEVEFDKLFEEKINDAFQYLNSILLEVKKSGKQPVLILDELQMIKDVVLNWQKYLLKELFQFLVSLTKEQHLCHVFCLSSDSLFIEYVYSAGELEGRAKYLLVDDFDKETALKFMDFLAKEILNKKLSDEDKELIYNYVGGKPVYIYSVIDEMRYRKLEDILNLMLKEETQKLKYFLKELDYIKPKVELKDEIIEIKKDDIINALKLFKENYEVSDDDIPEPVYIYLVKKNILFLNPIEGILKPQSFLIWNAIKKLLNGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q57644</accession>
    <name>Y185_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0185</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0185</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64323">
        <property type="entry name" value="B64323"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57644"/>
    <dbReference type="SMR" id="Q57644"/>
    <dbReference type="STRING" id="243232.MJ_0185"/>
    <dbReference type="EnsemblBacteria" id="AAB98182">
        <property type="protein sequence ID" value="AAB98182"/>
        <property type="gene ID" value="MJ_0185"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0185"/>
    <dbReference type="HOGENOM" id="CLU_3130849_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106734" description="Uncharacterized protein MJ0185">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="49" mass="5332" checksum="D1594F2AAD47CEF4" modified="1996-11-01" version="1">MCYGIIVMILFSLYKGLAPNSAIVGISIMIIIAIGIYLIIEYAIKKIIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q57659</accession>
    <name>Y206_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0206</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0206</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64325">
        <property type="entry name" value="G64325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869701.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57659"/>
    <dbReference type="SMR" id="Q57659"/>
    <dbReference type="STRING" id="243232.MJ_0206"/>
    <dbReference type="EnsemblBacteria" id="AAB98190">
        <property type="protein sequence ID" value="AAB98190"/>
        <property type="gene ID" value="MJ_0206"/>
    </dbReference>
    <dbReference type="GeneID" id="1451055"/>
    <dbReference type="KEGG" id="mja:MJ_0206"/>
    <dbReference type="eggNOG" id="arCOG04171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135994_0_0_2"/>
    <dbReference type="InParanoid" id="Q57659"/>
    <dbReference type="OMA" id="AEHASTF"/>
    <dbReference type="OrthoDB" id="112766at2157"/>
    <dbReference type="PhylomeDB" id="Q57659"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007171">
        <property type="entry name" value="DUF371"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023131">
        <property type="entry name" value="Mth639-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40696">
        <property type="entry name" value="PTHR40696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04027">
        <property type="entry name" value="DUF371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106740" description="Uncharacterized protein MJ0206">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <sequence length="135" mass="15376" checksum="BBF1243507DE8A01" modified="1996-11-01" version="1">MEFIIKAKGHKNVSATHKTTLEITKEDYLTPTGHCIIGIDADKSMTDFSEEFKEKLRNAKKIIVEIEVEGIKDTIIGEGHKDLILNHPTDMVIRKSNYICPRTLMINANKSAKDINREIVKKLKEGKELIFKIIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q57670</accession>
    <name>VATA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>V-type ATP synthase alpha chain</fullName>
            <ecNumber>7.1.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>V-ATPase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">atpA</name>
        <name type="ordered locus">MJ0217</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98200" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="7.1.2.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98200.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64327">
        <property type="entry name" value="B64327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496424.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57670"/>
    <dbReference type="SMR" id="Q57670"/>
    <dbReference type="STRING" id="243232.MJ_0217"/>
    <dbReference type="EnsemblBacteria" id="AAB98200">
        <property type="protein sequence ID" value="AAB98200"/>
        <property type="gene ID" value="MJ_0217"/>
    </dbReference>
    <dbReference type="GeneID" id="1451067"/>
    <dbReference type="KEGG" id="mja:MJ_0217"/>
    <dbReference type="eggNOG" id="arCOG00868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008162_3_1_2"/>
    <dbReference type="InParanoid" id="Q57670"/>
    <dbReference type="OMA" id="RIVKTFW"/>
    <dbReference type="OrthoDB" id="6736at2157"/>
    <dbReference type="PhylomeDB" id="Q57670"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="PTHR43607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="SSF50615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144599" description="V-type ATP synthase alpha chain">
        <location>
            <begin position="1"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="234"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="587" mass="65557" checksum="26BDD64E6D7FEEC5" modified="2001-01-24" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57672</accession>
    <name>VATC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>V-type ATP synthase subunit C</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>V-ATPase subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">atpC</name>
        <name type="ordered locus">MJ0219</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98202" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98202.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64327">
        <property type="entry name" value="D64327"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57672"/>
    <dbReference type="SMR" id="Q57672"/>
    <dbReference type="STRING" id="243232.MJ_0219"/>
    <dbReference type="EnsemblBacteria" id="AAB98202">
        <property type="protein sequence ID" value="AAB98202"/>
        <property type="gene ID" value="MJ_0219"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0219"/>
    <dbReference type="eggNOG" id="arCOG02459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059311_0_0_2"/>
    <dbReference type="InParanoid" id="Q57672"/>
    <dbReference type="OMA" id="WKYTPYS"/>
    <dbReference type="PhylomeDB" id="Q57672"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00314">
        <property type="entry name" value="ATP_synth_C_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_su_c/d_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014272">
        <property type="entry name" value="ATPase_V0-cplx_c_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_su_c/d_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_suC/d"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="SSF103486"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02923">
        <property type="entry name" value="AhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000119366" description="V-type ATP synthase subunit C">
        <location>
            <begin position="1"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="381" mass="42777" checksum="D0D603481686D362" modified="2001-01-24" version="2">MTYFDNPLTLLIVVATIIIVLIVIVWITKMVIDLAPYAYVNARIRSKEAKLFDDAKLNELIESGSLEELVGLLEDTDYGQYVIEVMNELKDPVAVEKALDMYLADLYGLIYRISPDSAKKVLKVFAKKFDIKNIKTLIRAKFVGLSAEETYALLIPLGNIPVEKLKELAEVKTVEEVVRGLDGTEYFKILQEELSNYDQTSNIIGFELALDKYYLESLRKTIMTEGKEEDIFREFVGTIIDVENLKVILKGKADGLSAEELSKYVTLTGYELADWKLKDLMSAGGIEGVLSGLEGTSYAEVLAEAMEEYEKTKSIYAFEKALDKFVLEKGKKLSTRKPFGVGPIIGLIVSKELEVKNLKAIIKGKIENLKPEEIRSLLISL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q57681</accession>
    <name>SYG_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glycyl-tRNA synthetase</fullName>
            <shortName evidence="1">GlyRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">glyS</name>
        <name type="ordered locus">MJ0228</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glycine to tRNA(Gly).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)</text>
            <dbReference type="Rhea" id="RHEA:16013"/>
            <dbReference type="Rhea" id="RHEA-COMP:9664"/>
            <dbReference type="Rhea" id="RHEA-COMP:9683"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.14"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.14" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64328">
        <property type="entry name" value="E64328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869726.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57681"/>
    <dbReference type="SMR" id="Q57681"/>
    <dbReference type="STRING" id="243232.MJ_0228"/>
    <dbReference type="PRIDE" id="Q57681"/>
    <dbReference type="EnsemblBacteria" id="AAB98213">
        <property type="protein sequence ID" value="AAB98213"/>
        <property type="gene ID" value="MJ_0228"/>
    </dbReference>
    <dbReference type="GeneID" id="1451081"/>
    <dbReference type="KEGG" id="mja:MJ_0228"/>
    <dbReference type="eggNOG" id="arCOG00405">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015515_1_0_2"/>
    <dbReference type="InParanoid" id="Q57681"/>
    <dbReference type="OMA" id="EPSYGID"/>
    <dbReference type="OrthoDB" id="32623at2157"/>
    <dbReference type="PhylomeDB" id="Q57681"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00774">
        <property type="entry name" value="GlyRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00253_A">
        <property type="entry name" value="Gly_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027031">
        <property type="entry name" value="Gly-tRNA_synthase/POLG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022960">
        <property type="entry name" value="Gly_tRNA_ligase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033731">
        <property type="entry name" value="GlyRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002315">
        <property type="entry name" value="tRNA-synt_gly"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10745">
        <property type="entry name" value="PTHR10745"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01043">
        <property type="entry name" value="TRNASYNTHGLY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00389">
        <property type="entry name" value="glyS_dimeric"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000072990" description="Glycine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="206"/>
            <end position="211"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="211"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="328"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="447"/>
            <end position="451"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="451"/>
            <end position="454"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00253"/>
        </source>
    </evidence>
    <sequence length="577" mass="67121" checksum="598F8D2D9A57C61F" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q57689</accession>
    <name>SYR_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Arginine--tRNA ligase</fullName>
            <ecNumber>6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Arginyl-tRNA synthetase</fullName>
            <shortName>ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">argS</name>
        <name type="ordered locus">MJ0237</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg)</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64329">
        <property type="entry name" value="F64329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869735.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57689"/>
    <dbReference type="SMR" id="Q57689"/>
    <dbReference type="STRING" id="243232.MJ_0237"/>
    <dbReference type="EnsemblBacteria" id="AAB98223">
        <property type="protein sequence ID" value="AAB98223"/>
        <property type="gene ID" value="MJ_0237"/>
    </dbReference>
    <dbReference type="GeneID" id="1451091"/>
    <dbReference type="KEGG" id="mja:MJ_0237"/>
    <dbReference type="eggNOG" id="arCOG00487">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_1_2"/>
    <dbReference type="InParanoid" id="Q57689"/>
    <dbReference type="OMA" id="NKPLHLG"/>
    <dbReference type="OrthoDB" id="7046at2157"/>
    <dbReference type="PhylomeDB" id="Q57689"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
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    <keyword id="KW-0436">Ligase</keyword>
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        <location>
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    <evidence type="ECO:0000250" key="1"/>
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    <sequence length="566" mass="64979" checksum="E02A48181481D655" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q57695</accession>
    <name>DAPA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="1">HTPA synthase</shortName>
            <ecNumber evidence="1">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapA</name>
        <name type="ordered locus">MJ0244</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Acta Crystallogr. F Struct. Biol. Commun." volume="65" first="1222" last="1226">
            <title>Structure of dihydrodipicolinate synthase from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Padmanabhan B."/>
                <person name="Strange R.W."/>
                <person name="Antonyuk S.V."/>
                <person name="Ellis M.J."/>
                <person name="Hasnain S.S."/>
                <person name="Iino H."/>
                <person name="Agari Y."/>
                <person name="Bessho Y."/>
                <person name="Yokoyama S."/>
            </authorList>
            <dbReference type="PubMed" id="20054116"/>
            <dbReference type="DOI" id="10.1107/S174430910904651X"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="resolution" value="2.20 A"/>
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        <property type="reaction ID" value="UER00017"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
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        <property type="entry name" value="Aldolase_TIM"/>
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        <property type="entry name" value="DapA-like"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000103197" description="4-hydroxy-tetrahydrodipicolinate synthase">
        <location>
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    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
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        <ligand>
            <name>pyruvate</name>
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        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
            <position position="43"/>
        </location>
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    <feature type="site" description="Part of a proton relay during catalysis" evidence="1">
        <location>
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        </location>
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        <location>
            <begin position="4"/>
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    <sequence length="289" mass="31580" checksum="34CD8619C4A53CCA" modified="1996-11-01" version="1">MFKGVYPAIITPFKNKEVDFDGLEENINFLIENGVSGIVAVGTTGESPTLSHEEHKKVIEKVVDVVNGRVQVIAGAGSNCTEEAIELSVFAEDVGADAVLSITPYYNKPTQEGLRKHFGKVAESINLPIVLYNVPSRTAVNLEPKTVKLLAEEYSNISAVKEANPNLSQVSELIHDAKITVLSGNDELTLPIIALGGKGVISVVANIVPKEFVEMVNYALEGDFEKAREIHYKLFPLMKAMFIETNPIPVKTALNMMGRPAGELRLPLCEMSEEHKKILENVLKDLGLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q57709</accession>
    <name>NCPP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable inosine/xanthosine triphosphatase</fullName>
            <shortName evidence="1">ITPase/XTPase</shortName>
            <ecNumber evidence="1">3.6.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-standard purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate phosphatase</fullName>
            <shortName evidence="1">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0261</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + XTP = H(+) + phosphate + XDP</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + ITP = H(+) + IDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the YjjX NTPase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98247" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98247.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64332">
        <property type="entry name" value="F64332"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57709"/>
    <dbReference type="SMR" id="Q57709"/>
    <dbReference type="STRING" id="243232.MJ_0261"/>
    <dbReference type="EnsemblBacteria" id="AAB98247">
        <property type="protein sequence ID" value="AAB98247"/>
        <property type="gene ID" value="MJ_0261"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0261"/>
    <dbReference type="eggNOG" id="arCOG01221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087417_0_1_2"/>
    <dbReference type="InParanoid" id="Q57709"/>
    <dbReference type="OMA" id="ADYWVGI"/>
    <dbReference type="PhylomeDB" id="Q57709"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="SSF52972"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00258">
        <property type="entry name" value="TIGR00258"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156359" description="Probable inosine/xanthosine triphosphatase">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="179" mass="19374" checksum="DFAF307795C3633F" modified="2003-06-06" version="4">MARHKLRIVAVGSTNPVKIEAVKEGFEKVLGAVEVIGVDVISGVSSHPIGLEETYLGALNRAKNAFEKVQCTYAVGIEAGLIKVGEHYIDIHICVVFDGVNETVGLSQGFEYPKIVAEKVLEGIEGGKIAEEISGIKDIGKNIGLIGYLTDNNITRKDLCRESVIMALIPRMIKNAHLY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="145" xmlns="http://uniprot.org/uniprot">
    <accession>Q57710</accession>
    <name>IF2P_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable translation initiation factor IF-2</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Mja infB intein</fullName>
            </recommendedName>
            <alternativeName>
                <fullName>Mja IF2 intein</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name type="primary">infB</name>
        <name type="ordered locus">MJ0262</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The intein interrupts the GTP-binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64332">
        <property type="entry name" value="G64332"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869759.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57710"/>
    <dbReference type="SMR" id="Q57710"/>
    <dbReference type="STRING" id="243232.MJ_0262"/>
    <dbReference type="EnsemblBacteria" id="AAB98248">
        <property type="protein sequence ID" value="AAB98248"/>
        <property type="gene ID" value="MJ_0262"/>
    </dbReference>
    <dbReference type="GeneID" id="1451116"/>
    <dbReference type="KEGG" id="mja:MJ_0262"/>
    <dbReference type="eggNOG" id="arCOG01560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002656_3_4_2"/>
    <dbReference type="InParanoid" id="Q57710"/>
    <dbReference type="OMA" id="DHGKCLL"/>
    <dbReference type="OrthoDB" id="604at2157"/>
    <dbReference type="PhylomeDB" id="Q57710"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10050">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00100_A">
        <property type="entry name" value="IF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029459">
        <property type="entry name" value="EFTU-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004544">
        <property type="entry name" value="TF_aIF-2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015760">
        <property type="entry name" value="TIF_IF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023115">
        <property type="entry name" value="TIF_IF2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036925">
        <property type="entry name" value="TIF_IF2_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039518">
        <property type="entry name" value="WhiA_LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43381">
        <property type="entry name" value="PTHR43381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GTP_EFTU_D4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14527">
        <property type="entry name" value="LAGLIDADG_WhiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00491">
        <property type="entry name" value="aIF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000014484" description="Probable translation initiation factor IF-2, 1st part" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014485" description="Mja infB intein" evidence="2">
        <location>
            <begin position="31"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014486" description="Probable translation initiation factor IF-2, 2nd part" evidence="2">
        <location>
            <begin position="577"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease">
        <location>
            <begin position="237"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="561"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="634"/>
            <end position="638"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="688"/>
            <end position="691"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1155" mass="132299" checksum="6653FA355E7EE0D3" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57718</accession>
    <name>Y270_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0270</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0270</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64333">
        <property type="entry name" value="G64333"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57718"/>
    <dbReference type="SMR" id="Q57718"/>
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    <dbReference type="EnsemblBacteria" id="AAB98259">
        <property type="protein sequence ID" value="AAB98259"/>
        <property type="gene ID" value="MJ_0270"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0270"/>
    <dbReference type="HOGENOM" id="CLU_2613655_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106763" description="Uncharacterized protein MJ0270">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="78" mass="8962" checksum="85CB456F9F271F08" modified="1996-11-01" version="1">MKNKREKMRPKSSTILILLMSVLILLLSIDILANHIIIKVDGYYYDGLGQKLAMKDVIPINASFNKIKSQLEKSMKFH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q57722</accession>
    <name>Y274_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0274</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0274</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64334">
        <property type="entry name" value="C64334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57722"/>
    <dbReference type="STRING" id="243232.MJ_0274"/>
    <dbReference type="EnsemblBacteria" id="AAB98258">
        <property type="protein sequence ID" value="AAB98258"/>
        <property type="gene ID" value="MJ_0274"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0274"/>
    <dbReference type="eggNOG" id="arCOG01359">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_533842_0_0_2"/>
    <dbReference type="InParanoid" id="Q57722"/>
    <dbReference type="OMA" id="HTPGKYL"/>
    <dbReference type="PhylomeDB" id="Q57722"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000106766" description="Uncharacterized protein MJ0274">
        <location>
            <begin position="1"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="203"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="470"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="543" mass="62048" checksum="8D2D2966EC84D4DA" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q57735</accession>
    <name>Y287_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0287</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0287</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64335">
        <property type="entry name" value="H64335"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869785.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57735"/>
    <dbReference type="SMR" id="Q57735"/>
    <dbReference type="STRING" id="243232.MJ_0287"/>
    <dbReference type="EnsemblBacteria" id="AAB98274">
        <property type="protein sequence ID" value="AAB98274"/>
        <property type="gene ID" value="MJ_0287"/>
    </dbReference>
    <dbReference type="GeneID" id="1451142"/>
    <dbReference type="KEGG" id="mja:MJ_0287"/>
    <dbReference type="eggNOG" id="arCOG01055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159725_2_1_2"/>
    <dbReference type="InParanoid" id="Q57735"/>
    <dbReference type="OMA" id="RIMYAAN"/>
    <dbReference type="OrthoDB" id="119868at2157"/>
    <dbReference type="PhylomeDB" id="Q57735"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106774" description="Uncharacterized protein MJ0287">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <sequence length="97" mass="11764" checksum="87120EAF43973F6B" modified="1996-11-01" version="1">MRIPPPLSNNKNRNRRYKRSQFEVIFEILHIIKEGEQIKTRIMYAANLDWRNFSKYIDFLISNGFIKKNKEKFELTELGKKLYSSLYELFEIMNSKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57738</accession>
    <name>Y290_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0290</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0290</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64336">
        <property type="entry name" value="C64336"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869788.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57738"/>
    <dbReference type="SMR" id="Q57738"/>
    <dbReference type="STRING" id="243232.MJ_0290"/>
    <dbReference type="EnsemblBacteria" id="AAB98283">
        <property type="protein sequence ID" value="AAB98283"/>
        <property type="gene ID" value="MJ_0290"/>
    </dbReference>
    <dbReference type="GeneID" id="1451145"/>
    <dbReference type="KEGG" id="mja:MJ_0290"/>
    <dbReference type="eggNOG" id="arCOG01055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313130_0_0_2"/>
    <dbReference type="OMA" id="KTFRNYI"/>
    <dbReference type="OrthoDB" id="119868at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038723">
        <property type="entry name" value="ArnR1-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14947">
        <property type="entry name" value="HTH_45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106777" description="Uncharacterized protein MJ0290">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <sequence length="211" mass="24620" checksum="FB64861E06896025" modified="1996-11-01" version="1">MKCISKQGEIKELIKNGKINDVLQLIEEDTLLLEEIYGFLKSDDIQLKITCLAILGNLYLKGKVQITQLIKHLEEVLLENDKDAILNALLILKEIPEVYQEDLLKRIILKYIGKDIKDCEDDKDKSTLPSVKRDKIMIIFEILKAVKNKELKKTKIMYAANLDWKTFRNYIGYLLDNEFIRKTDGVYTLTPKGELLLEKIEEVFRLIYPDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57745</accession>
    <name>Y297_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0297</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0297</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64337">
        <property type="entry name" value="B64337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869795.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57745"/>
    <dbReference type="EnsemblBacteria" id="AAB98285">
        <property type="protein sequence ID" value="AAB98285"/>
        <property type="gene ID" value="MJ_0297"/>
    </dbReference>
    <dbReference type="GeneID" id="1451152"/>
    <dbReference type="KEGG" id="mja:MJ_0297"/>
    <dbReference type="eggNOG" id="arCOG09664">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105213_0_0_2"/>
    <dbReference type="OMA" id="RIVNYME"/>
    <dbReference type="OrthoDB" id="88787at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106780" description="Uncharacterized protein MJ0297">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <sequence length="251" mass="29568" checksum="931564A2C27ADDDC" modified="1996-11-01" version="1">MMKYRQKDDKMNFENENALFKKALEEKEKGNYDDAIYYLDWASLIAFAKGNLQKIKEIEKILSELVEKTDYLSLYASFFIKITNSILKKEKLPNNIIDEFFEAIEGIEEKDKEFKFVVMALKRIVNYMEPMNQKVPEWIYEWIEDKEEMIKEVEKFNPEKDKVLIQSKDFKKGFVTGTFIGGELDKSKMKIVERAKMMFGIIEVDGAVIEIPLMAMNFTGGIFRAKGVKNEEHLNKIIKTIEDLMIDSYFY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q57800</accession>
    <name>Y354_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0354</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0354</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64344">
        <property type="entry name" value="B64344"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57800"/>
    <dbReference type="SMR" id="Q57800"/>
    <dbReference type="STRING" id="243232.MJ_0354"/>
    <dbReference type="EnsemblBacteria" id="AAB98343">
        <property type="protein sequence ID" value="AAB98343"/>
        <property type="gene ID" value="MJ_0354"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0354"/>
    <dbReference type="eggNOG" id="arCOG09666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2968474_0_0_2"/>
    <dbReference type="OMA" id="ERAWSDY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106825" description="Uncharacterized protein MJ0354">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <sequence length="58" mass="6781" checksum="354AC901F74A6500" modified="1996-11-01" version="1">MQINKAIELLERAWSDYNNGDTVGAILKLEEAEDLIRKLRVRLCSEIRREGYDAIFIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q57801</accession>
    <name>Y355_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0355</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0355</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64344">
        <property type="entry name" value="C64344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869854.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57801"/>
    <dbReference type="SMR" id="Q57801"/>
    <dbReference type="STRING" id="243232.MJ_0355"/>
    <dbReference type="EnsemblBacteria" id="AAB98347">
        <property type="protein sequence ID" value="AAB98347"/>
        <property type="gene ID" value="MJ_0355"/>
    </dbReference>
    <dbReference type="GeneID" id="1451212"/>
    <dbReference type="KEGG" id="mja:MJ_0355"/>
    <dbReference type="eggNOG" id="arCOG09656">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177887_0_0_2"/>
    <dbReference type="OrthoDB" id="137476at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106826" description="Uncharacterized protein MJ0355">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <sequence length="109" mass="12942" checksum="8EFACB812E200839" modified="1996-11-01" version="1">MLRFKKTPDEVKEMIKLAIKKGYRQIKISSVNPNEFLLRFDGRMWIMGFEFLSDKEIKYSCFENYSFKCILETDAKKVREFLEEIYSKSKIVNGRVKEIPTDVFQVVVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q57804</accession>
    <name>Y358_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0358</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0358</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64344">
        <property type="entry name" value="F64344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869857.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57804"/>
    <dbReference type="SMR" id="Q57804"/>
    <dbReference type="STRING" id="243232.MJ_0358"/>
    <dbReference type="EnsemblBacteria" id="AAB98350">
        <property type="protein sequence ID" value="AAB98350"/>
        <property type="gene ID" value="MJ_0358"/>
    </dbReference>
    <dbReference type="GeneID" id="1451215"/>
    <dbReference type="KEGG" id="mja:MJ_0358"/>
    <dbReference type="HOGENOM" id="CLU_1954678_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106829" description="Uncharacterized protein MJ0358">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <sequence length="128" mass="15084" checksum="CEE12E04BBC2FA59" modified="1996-11-01" version="1">MTFDLLKELKRIYKHFENIDLEQFERDLIECGFGKIKPGPLATDEVLTEEDIAKYREIIMKSQTCNVNTHNQIVIKINLKEINADNKMYCKFRKGYKFKEDIKVGEESSYIITLQQDSLHICNNYEVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q57813</accession>
    <name>Y367_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable integrase/recombinase protein MJ0367</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0367</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the 'phage' integrase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64345">
        <property type="entry name" value="G64345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869866.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57813"/>
    <dbReference type="SMR" id="Q57813"/>
    <dbReference type="STRING" id="243232.MJ_0367"/>
    <dbReference type="DNASU" id="1451224"/>
    <dbReference type="EnsemblBacteria" id="AAB98346">
        <property type="protein sequence ID" value="AAB98346"/>
        <property type="gene ID" value="MJ_0367"/>
    </dbReference>
    <dbReference type="GeneID" id="1451224"/>
    <dbReference type="KEGG" id="mja:MJ_0367"/>
    <dbReference type="eggNOG" id="arCOG01241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027562_9_5_2"/>
    <dbReference type="InParanoid" id="Q57813"/>
    <dbReference type="OMA" id="HSFASHM"/>
    <dbReference type="OrthoDB" id="57897at2157"/>
    <dbReference type="PhylomeDB" id="Q57813"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075713">
        <property type="term" value="P:establishment of integrated proviral latency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046718">
        <property type="term" value="P:viral entry into host cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044826">
        <property type="term" value="P:viral genome integration into host DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044068">
        <property type="entry name" value="CB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002104">
        <property type="entry name" value="Integrase_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004107">
        <property type="entry name" value="Integrase_SAM-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13495">
        <property type="entry name" value="Phage_int_SAM_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00589">
        <property type="entry name" value="Phage_integrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="SSF56349"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51900">
        <property type="entry name" value="CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51898">
        <property type="entry name" value="TYR_RECOMBINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0229">DNA integration</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1179">Viral genome integration</keyword>
    <keyword id="KW-1160">Virus entry into host cell</keyword>
    <feature type="chain" id="PRO_0000197583" description="Probable integrase/recombinase protein MJ0367">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="Core-binding (CB)" evidence="2">
        <location>
            <begin position="22"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Tyr recombinase" evidence="1">
        <location>
            <begin position="136"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="301"/>
        </location>
    </feature>
    <feature type="active site" description="O-(3'-phospho-DNA)-tyrosine intermediate" evidence="1">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="330" mass="39424" checksum="F397DBF4B9A54531" modified="1996-11-01" version="1">MREKELKHIENLLLLKVKKEKIEETDKIKEYLKRFEDERRFDGIKESTIKSDLDRLRVFLDYCINYLGKNPEELKTSDFVKFFNYLDTVRKVSKSSQRKYFLLLKVFYRVLRMYNVIQEFVEESKDRKRFARIEIQHYDAVDAEMLNMILKKIIESGSRTRIRDALIIRLLWDTGCRVSEVLNLKYKDCDLDNGIFKIRNTKTHEERTVVCSSDTLELLRNYVQFNVRQGSDDYLFQNSQGGRVRKEWISEVFRKAVNELKEEGKIPKNRRIVIHSIRHGRAVDLLNKGVPIDIVKEYLGHKSMNTTLIYAHSKERAESLEFIKKLLRIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q57814</accession>
    <name>Y368_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0368</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0368</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64345">
        <property type="entry name" value="H64345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869867.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57814"/>
    <dbReference type="SMR" id="Q57814"/>
    <dbReference type="STRING" id="243232.MJ_0368"/>
    <dbReference type="EnsemblBacteria" id="AAB98368">
        <property type="protein sequence ID" value="AAB98368"/>
        <property type="gene ID" value="MJ_0368"/>
    </dbReference>
    <dbReference type="GeneID" id="1451225"/>
    <dbReference type="KEGG" id="mja:MJ_0368"/>
    <dbReference type="eggNOG" id="arCOG12717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271090_0_0_2"/>
    <dbReference type="OMA" id="WEKINKM"/>
    <dbReference type="OrthoDB" id="138183at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106836" description="Uncharacterized protein MJ0368">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <sequence length="106" mass="12705" checksum="4EA3F47CB582668A" modified="1996-11-01" version="1">MGGDMTFDEFESLKNYPPKESIKEEKRVFNPKNIEVGNNFIKIEDIKIIFSKDIEKGEEEYLLMDVILSSLEGIYNLIEDEVYKKEIKKIWEKINEIRNYYNPEFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="152" xmlns="http://uniprot.org/uniprot">
    <accession>Q57815</accession>
    <name>TOP6A_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="2 3">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">top6A</name>
        <name type="ordered locus">MJ0369</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="EMBO J." volume="18" first="6177" last="6188">
            <title>Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11.</title>
            <authorList>
                <person name="Nichols M.D."/>
                <person name="DeAngelis K."/>
                <person name="Keck J.L."/>
                <person name="Berger J.M."/>
            </authorList>
            <dbReference type="PubMed" id="10545127"/>
            <dbReference type="DOI" id="10.1093/emboj/18.21.6177"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-69</scope>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 69-369 IN COMPLEX WITH MAGNESIUM IONS</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF GLU-197</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Mg(2+) is directly coordinated by Glu-197 and Asp-249 as well as indirectly coordinated through 2 water molecules by Asp-251 (PubMed:10545127).</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Homodimer (PubMed:10545127). Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TOP6A family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="2 3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64346">
        <property type="entry name" value="A64346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869868.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1D3Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=69-369"/>
    </dbReference>
    <dbReference type="PDBsum" id="1D3Y"/>
    <dbReference type="AlphaFoldDB" id="Q57815"/>
    <dbReference type="SMR" id="Q57815"/>
    <dbReference type="STRING" id="243232.MJ_0369"/>
    <dbReference type="EnsemblBacteria" id="AAB98358">
        <property type="protein sequence ID" value="AAB98358"/>
        <property type="gene ID" value="MJ_0369"/>
    </dbReference>
    <dbReference type="GeneID" id="1451226"/>
    <dbReference type="KEGG" id="mja:MJ_0369"/>
    <dbReference type="eggNOG" id="arCOG04143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037229_1_0_2"/>
    <dbReference type="InParanoid" id="Q57815"/>
    <dbReference type="OMA" id="NWGEARF"/>
    <dbReference type="OrthoDB" id="22842at2157"/>
    <dbReference type="PhylomeDB" id="Q57815"/>
    <dbReference type="EvolutionaryTrace" id="Q57815"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="PTHR10848"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="SSF56726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <feature type="chain" id="PRO_0000145449" description="Type 2 DNA topoisomerase 6 subunit A">
        <location>
            <begin position="1"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Reduces affinity for DNA." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="98"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="147"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="153"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="161"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="173"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="191"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="199"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="210"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="216"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="244"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="252"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="258"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="282"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="288"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="317"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="337"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="341"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="351"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="356"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="364"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9M4A2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10545127"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1D3Y"/>
        </source>
    </evidence>
    <sequence length="369" mass="41829" checksum="7F75282B3E547834" modified="1996-11-01" version="1">MVKLPAISKKPREIAKQKIIELAKKMYEDLMKGKRPKITMPIRSLSNAMFDKEKGSFTLVGKEKARTLTVNQAKIFAQTTKMLEFAKQLLETDDFSTLREAYYVSKNWGEARFDDQQASNNVIEDLEAALGVLREHLGFIPEEDGSSVVGPLKIIEETPEGELVVDCTKLGTGAYNIPNDVTKLNLETDADFILAIETSGMFARLNAERFWDKHNCILVSLKGVPARATRRFIKRLHEEHDLPVLVFTDGDPYGYLNIYRTLKVGSGKAIHLADKLSIPAARLIGVTPQDIIDYDLPTHPLKEQDIKRIKDGLKNDDFVRSFPEWQKALKQMLDMGVRAEQQSLAKYGLKYVVNTYLPEKIKDESTWLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q57856</accession>
    <name>Y413_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ABC transporter permease protein MJ0413</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0413</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Probably part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64351">
        <property type="entry name" value="E64351"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57856"/>
    <dbReference type="SMR" id="Q57856"/>
    <dbReference type="STRING" id="243232.MJ_0413"/>
    <dbReference type="EnsemblBacteria" id="AAB98402">
        <property type="protein sequence ID" value="AAB98402"/>
        <property type="gene ID" value="MJ_0413"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0413"/>
    <dbReference type="eggNOG" id="arCOG00169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046113_1_4_2"/>
    <dbReference type="InParanoid" id="Q57856"/>
    <dbReference type="OMA" id="LIEFYRP"/>
    <dbReference type="PhylomeDB" id="Q57856"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060305" description="Putative ABC transporter permease protein MJ0413">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="1">
        <location>
            <begin position="71"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="267" mass="29043" checksum="2A6B9AAEC07A4111" modified="1996-11-01" version="1">MMNHKKGISVKINTKELVLKISLPALAVVIWELLAIYINNPVILPRVEAVINVLIHPFQGILGTGSLIDNTIISIKRVISGFLLASAVAIPLGILMGYYRTVNSLCDTLIELLRPIPPLAWVPLSLAWFGLGEMSMIFIIFIGAFFPILINTISGVKGVPTPLIEAALTLGAKGRDILIKVVIPASSPSILTGLRVGAGIAWMCVVAAEMLPSSNAGLGYLIMYAYSLSRMDVVIACMIIIGLIGLVLDRGLRYIEDKYFVWRKMMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q57866</accession>
    <name>Y423_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0423</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0423</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64352">
        <property type="entry name" value="G64352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869922.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57866"/>
    <dbReference type="STRING" id="243232.MJ_0423"/>
    <dbReference type="EnsemblBacteria" id="AAB98411">
        <property type="protein sequence ID" value="AAB98411"/>
        <property type="gene ID" value="MJ_0423"/>
    </dbReference>
    <dbReference type="GeneID" id="63606500"/>
    <dbReference type="KEGG" id="mja:MJ_0423"/>
    <dbReference type="eggNOG" id="arCOG03166">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_210066_0_0_2"/>
    <dbReference type="OrthoDB" id="21452at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106868" description="Uncharacterized ATP-binding protein MJ0423">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="59" mass="7209" checksum="8DFBC8C9CBD610EA" modified="1996-11-01" version="1">MYGIVMFVNRKEELEFLERKWNENKANLIILYGRRRVGKTMLIKKFLENKKIKRASIFC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q57897</accession>
    <name>Y455_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0455</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0455</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64356">
        <property type="entry name" value="G64356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869954.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57897"/>
    <dbReference type="STRING" id="243232.MJ_0455"/>
    <dbReference type="EnsemblBacteria" id="AAB98448">
        <property type="protein sequence ID" value="AAB98448"/>
        <property type="gene ID" value="MJ_0455"/>
    </dbReference>
    <dbReference type="GeneID" id="1451316"/>
    <dbReference type="KEGG" id="mja:MJ_0455"/>
    <dbReference type="eggNOG" id="arCOG05003">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091350_1_0_2"/>
    <dbReference type="InParanoid" id="Q57897"/>
    <dbReference type="OMA" id="GLCIGHD"/>
    <dbReference type="OrthoDB" id="78172at2157"/>
    <dbReference type="PhylomeDB" id="Q57897"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014997">
        <property type="entry name" value="DUF1847"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08901">
        <property type="entry name" value="DUF1847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106884" description="Uncharacterized protein MJ0455">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <sequence length="193" mass="22113" checksum="655279BEE3396404" modified="1996-11-01" version="1">MKCSQCNKKLCYTGKDCKKDITQKIIEEYKKEENLKIAEVSAYIEATYYMKKTRLEEIIEFCKLMEYKKIGIAFCIGLENEAKILAKILSKHFEVYSVCCKVCGIDKDVFKFKKINKGEKEAMCNPIGQAEILNEIGTDLNIIVGLCIGHDILFQKYSKAPTTTFIVKDRVLSHNTAGAIYTKYYLKKLLEGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57905</accession>
    <name>Y482_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0482</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0482</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64360">
        <property type="entry name" value="A64360"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57905"/>
    <dbReference type="SMR" id="Q57905"/>
    <dbReference type="STRING" id="243232.MJ_0482"/>
    <dbReference type="DNASU" id="1451343"/>
    <dbReference type="EnsemblBacteria" id="AAB98473">
        <property type="protein sequence ID" value="AAB98473"/>
        <property type="gene ID" value="MJ_0482"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0482"/>
    <dbReference type="eggNOG" id="arCOG02408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100862_0_0_2"/>
    <dbReference type="InParanoid" id="Q57905"/>
    <dbReference type="OMA" id="TIAYVTR"/>
    <dbReference type="PhylomeDB" id="Q57905"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002790">
        <property type="entry name" value="CHP00288"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN_MARF1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00288">
        <property type="entry name" value="TIGR00288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106890" description="Uncharacterized protein MJ0482">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <sequence length="240" mass="27868" checksum="3192B56BDBE60D48" modified="1996-11-01" version="1">MKSPHKVIYCYLIILYKLSSCHIYYKYIPFRLTVLVNYFLLLNVGEIMWKKLESLTSKIYEKARKRKGEHRIALLIDGPNMLRKEFNIDLDKIREVLSEFGDIVIGRVYLNQYASDKLIEAVINQGFEPKISAGDVDVEMAVDATELVFNPNIDTIAYVTRDADFLPAIRKAKERGKKVIVIGAEPGFSTALQNIADYVIKIGEEFQLDREKLEKKKKNKFLKVEEKQKDKEETENREEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57908</accession>
    <name>COBQ_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cobQ</name>
        <name type="ordered locus">MJ0484</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98475" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98475.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64360">
        <property type="entry name" value="D64360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496916.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57908"/>
    <dbReference type="STRING" id="243232.MJ_0484"/>
    <dbReference type="EnsemblBacteria" id="AAB98475">
        <property type="protein sequence ID" value="AAB98475"/>
        <property type="gene ID" value="MJ_0484"/>
    </dbReference>
    <dbReference type="GeneID" id="1451346"/>
    <dbReference type="KEGG" id="mja:MJ_0484"/>
    <dbReference type="eggNOG" id="arCOG00105">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019250_2_2_2"/>
    <dbReference type="InParanoid" id="Q57908"/>
    <dbReference type="OMA" id="EIHHGVA"/>
    <dbReference type="OrthoDB" id="34382at2157"/>
    <dbReference type="PhylomeDB" id="Q57908"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="PTHR21343:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141348" description="Probable cobyric acid synthase">
        <location>
            <begin position="1"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="domain" description="GATase cobBQ-type">
        <location>
            <begin position="252"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="492" mass="55419" checksum="0B8E8803B12E5F44" modified="2000-05-30" version="2">MAEFIMVVGTSSNSGKTTITAGLCRILANKGYKVAPFKSQNMSLNSRVAKEDGEIAIAQYTQSLACRVEPSVHFNPILLKPKGNFISQVIVHGRPYKDMNYNEYRKNKDFFLKKIKESLEILDREYDYVIMEGAGSCCEINLLKDDIANLRIAELVNAKAILVADIDRGGVFASIYGTIKLLPENWRKLIKGIIINKFRGNVEVLKEGIEKIEELTGIPVLGIVPYDENLVLPEEDSQVLQSMRSFGNAKSGVEINVVRFSKISNFTDLDPLRYDAFIKFIDFDDDITGDILIFPGTRSSTKEAYYLKQHNFDEKVLEFLKDGGIVIGICGGYQVLGKELIDKEKKESDVGDIEGLKIFDAKTYFGNDKVVKNSCGFLEIDNKTFNVKGYEIHEGFTYSKEKPLIKIERGFGNCGNGFDGSIKKFGDGLAIGTYFHGIFENYEFRNYIINLIRKRKGLDEIYGDSYKDSIEKSLNYFAEVVERSVNLKPLGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57917</accession>
    <name>RNP2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 2</fullName>
            <shortName evidence="1">RNase P component 2</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pop5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp2</name>
        <name type="ordered locus">MJ0494</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Nucleic Acids Res." volume="36" first="4172" last="4180">
            <title>Studies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysis.</title>
            <authorList>
                <person name="Pulukkunat D.K."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="18558617"/>
            <dbReference type="DOI" id="10.1093/nar/gkn360"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP3</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="411" first="368" last="383">
            <title>Cooperative RNP assembly: complementary rescue of structural defects by protein and RNA subunits of archaeal RNase P.</title>
            <authorList>
                <person name="Chen W.Y."/>
                <person name="Xu Y."/>
                <person name="Cho I.M."/>
                <person name="Oruganti S.V."/>
                <person name="Foster M.P."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="21683084"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2011.05.012"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP3</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2012" name="Nucleic Acids Res." volume="40" first="4666" last="4680">
            <title>Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors.</title>
            <authorList>
                <person name="Chen W.Y."/>
                <person name="Singh D."/>
                <person name="Lai L.B."/>
                <person name="Stiffler M.A."/>
                <person name="Lai H.D."/>
                <person name="Foster M.P."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="22298511"/>
            <dbReference type="DOI" id="10.1093/nar/gks013"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP3</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 4">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4">Consists of a catalytic RNA component and at least 4-5 protein subunits. Forms a subcomplex with Rnp3 which stimulates the catalytic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64361">
        <property type="entry name" value="F64361"/>
    </dbReference>
    <dbReference type="PDB" id="6K0A">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.60 A"/>
        <property type="chains" value="A/B=1-134"/>
    </dbReference>
    <dbReference type="PDB" id="6K0B">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.30 A"/>
        <property type="chains" value="A/B=1-134"/>
    </dbReference>
    <dbReference type="PDBsum" id="6K0A"/>
    <dbReference type="PDBsum" id="6K0B"/>
    <dbReference type="AlphaFoldDB" id="Q57917"/>
    <dbReference type="SMR" id="Q57917"/>
    <dbReference type="STRING" id="243232.MJ_0494"/>
    <dbReference type="EnsemblBacteria" id="AAB98484">
        <property type="protein sequence ID" value="AAB98484"/>
        <property type="gene ID" value="MJ_0494"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0494"/>
    <dbReference type="eggNOG" id="arCOG01365">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137733_1_0_2"/>
    <dbReference type="InParanoid" id="Q57917"/>
    <dbReference type="OMA" id="NPWLIDY"/>
    <dbReference type="PhylomeDB" id="Q57917"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00755">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016434">
        <property type="entry name" value="Rnp2_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004952">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="SSF160350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000140020" description="Ribonuclease P protein component 2">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18558617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="21683084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22298511"/>
        </source>
    </evidence>
    <sequence length="134" mass="15941" checksum="5BD64AB14CF0D9B7" modified="1996-11-01" version="1">MIEMLKTLPPTLREKKRYIAFKILYDEELKEGEVVNLIRKAVLEYYGSWGTSKANPWLVYYDFPYGILRCQRDNVDYVKASLILIREFKEKPVNIICLGVSGTIRKAKIKFLGIKKPKRWFVIRRERLKAKKQK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57918</accession>
    <name>SELB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Selenocysteine-specific elongation factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>SelB translation factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">selB</name>
        <name type="ordered locus">MJ0495</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Mol. Biol." volume="299" first="351" last="358">
            <title>Identification and characterisation of the selenocysteine-specific translation factor SelB from the archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Rother M."/>
                <person name="Wilting R."/>
                <person name="Commans S."/>
                <person name="Boeck A."/>
            </authorList>
            <dbReference type="PubMed" id="10860743"/>
            <dbReference type="DOI" id="10.1006/jmbi.2000.3756"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. SelB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64361">
        <property type="entry name" value="G64361"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57918"/>
    <dbReference type="SMR" id="Q57918"/>
    <dbReference type="STRING" id="243232.MJ_0495"/>
    <dbReference type="EnsemblBacteria" id="AAB98485">
        <property type="protein sequence ID" value="AAB98485"/>
        <property type="gene ID" value="MJ_0495"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0495"/>
    <dbReference type="eggNOG" id="arCOG01564">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019148_1_0_2"/>
    <dbReference type="InParanoid" id="Q57918"/>
    <dbReference type="OMA" id="FGHIDHG"/>
    <dbReference type="PhylomeDB" id="Q57918"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="L35A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004535">
        <property type="entry name" value="Transl_elong_SelB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="SSF50465"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00475">
        <property type="entry name" value="selB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000091477" description="Selenocysteine-specific elongation factor">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G" evidence="2">
        <location>
            <begin position="7"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="G1" evidence="2">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="G2" evidence="2">
        <location>
            <begin position="48"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="G3" evidence="2">
        <location>
            <begin position="69"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="G4" evidence="2">
        <location>
            <begin position="123"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="G5" evidence="2">
        <location>
            <begin position="159"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="123"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10860743"/>
        </source>
    </evidence>
    <sequence length="469" mass="51849" checksum="F3C1863CAAF04A92" modified="1996-11-01" version="1">MVISMEMKNVNVGLFGHIDHGKTQLAKQLTEIASTSALDKPKESQKRGITIDLGFSSFTLDRYRITLVDAPGHSELIRTAIGAGNIIDAALLVVDAKEGPKTQTGEHLLVLDLLNIPTIVVINKIDIANDEEIKRTEMFMKQILNSTINLKNSKIIKISAKTGEGIGELKKELKNLLDSLDIKRDINSYLKMPIDHAFKIKGVGTVVTGTIHKGKVEVGDNLRILPINHEVKVKSIQCFKQDVSIAYAGDRVGMALMGVEPESLFRGCILTSEDTKLKVVDKFIAKVKILELFKYNLAPKMKVHINIGLLTVPATIIPYKIEKINDKEEPIILEEIKGGDSCYCIFKLEERVVVDEGDKILIMRLDLPPTTLRICGFGEVIDFGEVEVKKIVVKEGKVVKKKDKIYIEGLASSKTAGEKLIGGKVYIPDKNIWGVIKGTFGTKGALIAEFDEEVNGGEKVVLKRVRKWG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q57944</accession>
    <name>Y524_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0524</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0524</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64365">
        <property type="entry name" value="D64365"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57944"/>
    <dbReference type="SMR" id="Q57944"/>
    <dbReference type="STRING" id="243232.MJ_0524"/>
    <dbReference type="EnsemblBacteria" id="AAB98516">
        <property type="protein sequence ID" value="AAB98516"/>
        <property type="gene ID" value="MJ_0524"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0524"/>
    <dbReference type="eggNOG" id="arCOG04831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1639963_0_0_2"/>
    <dbReference type="OMA" id="YTRGMDT"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011313">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09879">
        <property type="entry name" value="DUF2106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019373">
        <property type="entry name" value="EhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106913" description="Uncharacterized protein MJ0524">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="158" mass="18293" checksum="4381CA8F0625E931" modified="1996-11-01" version="1">MRWVIMKKLGKIWNYLSKPEIVPRIFSVFLALVFIFGLLMPHYLNPNQLYPKPIPHSQTLKTPLAPYDRGGIPLKEPAELKAQYPQYEPNLGKITAYLTPIAEWIKDKTYYFGTTIVSTPGGILDEILYYTRGMDTVLESSILLISFIIFSWLFFNKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57971</accession>
    <name>Y551_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0551</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0551</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64368">
        <property type="entry name" value="G64368"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57971"/>
    <dbReference type="SMR" id="Q57971"/>
    <dbReference type="STRING" id="243232.MJ_0551"/>
    <dbReference type="DNASU" id="1451416"/>
    <dbReference type="EnsemblBacteria" id="AAB98543">
        <property type="protein sequence ID" value="AAB98543"/>
        <property type="gene ID" value="MJ_0551"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0551"/>
    <dbReference type="eggNOG" id="arCOG02059">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072599_0_1_2"/>
    <dbReference type="InParanoid" id="Q57971"/>
    <dbReference type="OMA" id="KVRMHDF"/>
    <dbReference type="PhylomeDB" id="Q57971"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009337">
        <property type="term" value="C:sulfite reductase complex (NADPH)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050311">
        <property type="term" value="F:sulfite reductase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016002">
        <property type="term" value="F:sulfite reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045169">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11493">
        <property type="entry name" value="PTHR11493"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000199969" description="Uncharacterized protein MJ0551">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="275" mass="31188" checksum="D4916B68EEA5882E" modified="1996-11-01" version="1">MVKRMHCNINLKYGVIMKKDCYTLRISLKPGFINAEQLKAIAYVIENFGDNKAHITTRQGIEFKISPEHLEEVEKILNNVGLNLGSTGNRVRQVVSCIGLECYNAIGDSVSLARRIHEEFEGVWVPRKVKINVSGCPNSCTFHRFCDIGICYRYKITINKEICTNCGKCKDFCDLNAIDWERKIIKDNCTGEGKCTGLCNAFKAERVISIFVGGKGGRIYKEGKHLIDLKNEDDVLFVIDELISLYAKFGKGRMADFVENYGIENLRNNIKELIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-10" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57977</accession>
    <name>NEP1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>16S rRNA Psi914 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">nep1</name>
        <name type="ordered locus">MJ0557</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Biomol. NMR. Assign." volume="3" first="251" last="254">
            <title>Backbone resonance assignments of the 48 kDa dimeric putative 18S rRNA-methyltransferase Nep1 from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Wurm J.P."/>
                <person name="Duchardt E."/>
                <person name="Meyer B."/>
                <person name="Leal B.Z."/>
                <person name="Kotter P."/>
                <person name="Entian K.D."/>
                <person name="Wohnert J."/>
            </authorList>
            <dbReference type="PubMed" id="19779849"/>
            <dbReference type="DOI" id="10.1007/s12104-009-9187-z"/>
        </citation>
        <scope>NMR STUDIES</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="Nucleic Acids Res." volume="38" first="2387" last="2398">
            <title>The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase.</title>
            <authorList>
                <person name="Wurm J.P."/>
                <person name="Meyer B."/>
                <person name="Bahr U."/>
                <person name="Held M."/>
                <person name="Frolow O."/>
                <person name="Kotter P."/>
                <person name="Engels J.W."/>
                <person name="Heckel A."/>
                <person name="Karas M."/>
                <person name="Entian K.D."/>
                <person name="Wohnert J."/>
            </authorList>
            <dbReference type="PubMed" id="20047967"/>
            <dbReference type="DOI" id="10.1093/nar/gkp1189"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>RNA-BINDING</scope>
        <scope>RNA-BINDING SITES</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2008" name="Nucleic Acids Res." volume="36" first="1542" last="1554">
            <title>The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site.</title>
            <authorList>
                <person name="Taylor A.B."/>
                <person name="Meyer B."/>
                <person name="Leal B.Z."/>
                <person name="Kotter P."/>
                <person name="Schirf V."/>
                <person name="Demeler B."/>
                <person name="Hart P.J."/>
                <person name="Entian K.D."/>
                <person name="Wohnert J."/>
            </authorList>
            <dbReference type="PubMed" id="18208838"/>
            <dbReference type="DOI" id="10.1093/nar/gkm1172"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF APOPROTEIN AND COMPLEXES WITH S-ADENOSYL-L-HOMOCYSTEINE AND SINEFUNGIN INHIBITOR</scope>
        <scope>SUBUNIT</scope>
        <scope>RNA-BINDING SITES</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of pseudouridine at position 914 (Psi914) in 16S rRNA. Is not able to methylate uridine at this position.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>pseudouridine(914) in M. jannaschii 16S rRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(914) in M. jannaschii 16S rRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:46716"/>
            <dbReference type="Rhea" id="RHEA-COMP:11640"/>
            <dbReference type="Rhea" id="RHEA-COMP:11641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64369">
        <property type="entry name" value="E64369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870061.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3BBD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="A/B=1-205"/>
    </dbReference>
    <dbReference type="PDB" id="3BBE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-205"/>
    </dbReference>
    <dbReference type="PDB" id="3BBH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.25 A"/>
        <property type="chains" value="A/B=1-205"/>
    </dbReference>
    <dbReference type="PDBsum" id="3BBD"/>
    <dbReference type="PDBsum" id="3BBE"/>
    <dbReference type="PDBsum" id="3BBH"/>
    <dbReference type="AlphaFoldDB" id="Q57977"/>
    <dbReference type="BMRB" id="Q57977"/>
    <dbReference type="SMR" id="Q57977"/>
    <dbReference type="STRING" id="243232.MJ_0557"/>
    <dbReference type="PRIDE" id="Q57977"/>
    <dbReference type="EnsemblBacteria" id="AAB98551">
        <property type="protein sequence ID" value="AAB98551"/>
        <property type="gene ID" value="MJ_0557"/>
    </dbReference>
    <dbReference type="GeneID" id="1451422"/>
    <dbReference type="KEGG" id="mja:MJ_0557"/>
    <dbReference type="eggNOG" id="arCOG04122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055846_1_3_2"/>
    <dbReference type="InParanoid" id="Q57977"/>
    <dbReference type="OMA" id="VYVHTRN"/>
    <dbReference type="OrthoDB" id="72065at2157"/>
    <dbReference type="PhylomeDB" id="Q57977"/>
    <dbReference type="BRENDA" id="2.1.1.257">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.1.1.260">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57977"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="SGD"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="PTHR12636"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000158616" description="Ribosomal RNA small subunit methyltransferase Nep1">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="141"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="185"/>
            <end position="190"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA">
        <location>
            <position position="54"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-54">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="3"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="17"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="36"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="40"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="69"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="76"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="85"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="100"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="117"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="126"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="137"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="150"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="157"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="164"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="192"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="18208838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="20047967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3BBD"/>
        </source>
    </evidence>
    <sequence length="205" mass="24082" checksum="8D3E8BFDCD502831" modified="1996-11-01" version="1">MTYNIILAKSALELIPEEIKNKIRKSRVYKYDILDSNYHYKAMEKLKDKEMRGRPDIIHISLLNILDSPINHEKKLNIYIHTYDDKVLKINPETRLPRNYFRFLGVMEKVLKGERNHLIKMEEKTLEDLLNEINAKKIAIMTKTGKLTHPKLLKEYDTFIIGGFPYGKLKINKEKVFGDIKEISIYNKGLMAWTVCGIICYSLSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57988</accession>
    <name>Y568_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0568</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0568</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the DtxR/MntR family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64370">
        <property type="entry name" value="H64370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870072.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57988"/>
    <dbReference type="SMR" id="Q57988"/>
    <dbReference type="STRING" id="243232.MJ_0568"/>
    <dbReference type="EnsemblBacteria" id="AAB98562">
        <property type="protein sequence ID" value="AAB98562"/>
        <property type="gene ID" value="MJ_0568"/>
    </dbReference>
    <dbReference type="GeneID" id="1451433"/>
    <dbReference type="KEGG" id="mja:MJ_0568"/>
    <dbReference type="eggNOG" id="arCOG02100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069532_4_1_2"/>
    <dbReference type="InParanoid" id="Q57988"/>
    <dbReference type="OMA" id="SWDAIDR"/>
    <dbReference type="OrthoDB" id="110491at2157"/>
    <dbReference type="PhylomeDB" id="Q57988"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000201124" description="Uncharacterized HTH-type transcriptional regulator MJ0568">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="HTH dtxR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="125" mass="14588" checksum="AC4703013A3F7577" modified="1996-11-01" version="1">MSQSIEDYLERIYLFIKENNRPIKTTELAKLLNIKPSAVTNMAKKLHRLGYVNYEPYIGITLTEKGIEEAKKILDKHKTIKIFLVEFLGLDEKTASEEACKLEHALSDEILERLKIFMEKFKDKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57992</accession>
    <name>Y572_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0572</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0572</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64371">
        <property type="entry name" value="D64371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870076.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57992"/>
    <dbReference type="SMR" id="Q57992"/>
    <dbReference type="STRING" id="243232.MJ_0572"/>
    <dbReference type="EnsemblBacteria" id="AAB98572">
        <property type="protein sequence ID" value="AAB98572"/>
        <property type="gene ID" value="MJ_0572"/>
    </dbReference>
    <dbReference type="GeneID" id="1451437"/>
    <dbReference type="KEGG" id="mja:MJ_0572"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2490514_0_0_2"/>
    <dbReference type="InParanoid" id="Q57992"/>
    <dbReference type="OMA" id="EARECFF"/>
    <dbReference type="OrthoDB" id="125646at2157"/>
    <dbReference type="PhylomeDB" id="Q57992"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0802">TPR repeat</keyword>
    <feature type="chain" id="PRO_0000106936" description="Uncharacterized protein MJ0572">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 1">
        <location>
            <begin position="8"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 2">
        <location>
            <begin position="42"/>
            <end position="75"/>
        </location>
    </feature>
    <sequence length="86" mass="10258" checksum="4B1EF4C549045020" modified="1996-11-01" version="1">MDENIKKAEYYYKKGVEVGNKGDVEKALEYFNKAIELNPFYRDAWFNKALALRILGRYEEARECFFRGLAVEKHLTHKKYNSDDEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q57997</accession>
    <name>Y577_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Universal stress protein MJ0577</fullName>
            <shortName>USP MJ0577</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0577</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Proc. Natl. Acad. Sci. U.S.A." volume="95" first="15189" last="15193">
            <title>Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics.</title>
            <authorList>
                <person name="Zarembinski T.I."/>
                <person name="Hung L.-W."/>
                <person name="Mueller-Dieckmann H.-J."/>
                <person name="Kim K.-K."/>
                <person name="Yokota H."/>
                <person name="Kim R."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="9860944"/>
            <dbReference type="DOI" id="10.1073/pnas.95.26.15189"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) IN COMPLEX WITH ATP</scope>
    </reference>
    <comment type="cofactor">
        <cofactor>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text>Binds 1 Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64372">
        <property type="entry name" value="A64372"/>
    </dbReference>
    <dbReference type="PDB" id="1MJH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B=1-162"/>
    </dbReference>
    <dbReference type="PDBsum" id="1MJH"/>
    <dbReference type="AlphaFoldDB" id="Q57997"/>
    <dbReference type="SMR" id="Q57997"/>
    <dbReference type="STRING" id="243232.MJ_0577"/>
    <dbReference type="EnsemblBacteria" id="AAB98568">
        <property type="protein sequence ID" value="AAB98568"/>
        <property type="gene ID" value="MJ_0577"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0577"/>
    <dbReference type="eggNOG" id="arCOG00449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_2_2"/>
    <dbReference type="InParanoid" id="Q57997"/>
    <dbReference type="OMA" id="ILCPTDY"/>
    <dbReference type="PhylomeDB" id="Q57997"/>
    <dbReference type="EvolutionaryTrace" id="Q57997"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147446" description="Universal stress protein MJ0577">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 3">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 3">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 3">
        <location>
            <begin position="127"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 3">
        <location>
            <begin position="141"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="7"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="35"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="67"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="98"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="122"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="141"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="154"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="9860944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007744" key="3">
        <source>
            <dbReference type="PDB" id="1MJH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1MJH"/>
        </source>
    </evidence>
    <sequence length="162" mass="18339" checksum="0A1060E6A2E6E1F4" modified="1996-11-01" version="1">MSVMYKKILYPTDFSETAEIALKHVKAFKTLKAEEVILLHVIDEREIKKRDIFSLLLGVAGLNKSVEEFENELKNKLTEEAKNKMENIKKELEDVGFKVKDIIVVGIPHEEIVKIAEDEGVDIIIMGSHGKTNLKEILLGSVTENVIKKSNKPVLVVKRKNS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58014</accession>
    <name>Y597_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0597</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0597</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64374">
        <property type="entry name" value="E64374"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58014"/>
    <dbReference type="STRING" id="243232.MJ_0597"/>
    <dbReference type="EnsemblBacteria" id="AAB98589">
        <property type="protein sequence ID" value="AAB98589"/>
        <property type="gene ID" value="MJ_0597"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0597"/>
    <dbReference type="eggNOG" id="arCOG00659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_833177_0_0_2"/>
    <dbReference type="OMA" id="CKFCYMS"/>
    <dbReference type="PhylomeDB" id="Q58014"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000106950" description="Uncharacterized protein MJ0597">
        <location>
            <begin position="1"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="27"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="357" mass="40900" checksum="BC16E3B3FFA774F4" modified="1996-11-01" version="1">MTIGREKMKVEEILENARKAFKLTTKHFGNTVTFERALFLGWYCNLKQPCKFCYMATQKNKIKDPRKARRRLESVLAEAILMKRIGWKLEFISGGYGYTPKEINDIAEMVAYVQKCRQYLNVGVIDLDNINLDVIEGVVGAVETVSKDRDWICPGKPLDKIKDNLLKAKELGLKTGITIILGLGEKEEDIEKLLNLIEELDLNRITFYSLNPQKGTIYENKPSVTTIEYMNWVSSVRLNFPKIKIITGVWVDKIPMISPLIMSGSNVITKFPLFSVFGTKEAHWIEKEILATGRELLGTFTDIDILAGKKVLEKTPYIEEEINISSENIKRVEELRENINERIESYVSKVLRKIKAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58030</accession>
    <name>END3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease III</fullName>
            <ecNumber evidence="1">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nth</name>
        <name type="ordered locus">MJ0613</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Nth/MutY family.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870118.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58030"/>
    <dbReference type="SMR" id="Q58030"/>
    <dbReference type="STRING" id="243232.MJ_0613"/>
    <dbReference type="EnsemblBacteria" id="AAB98606">
        <property type="protein sequence ID" value="AAB98606"/>
        <property type="gene ID" value="MJ_0613"/>
    </dbReference>
    <dbReference type="GeneID" id="1451479"/>
    <dbReference type="KEGG" id="mja:MJ_0613"/>
    <dbReference type="eggNOG" id="arCOG00459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_805637_0_0_2"/>
    <dbReference type="InParanoid" id="Q58030"/>
    <dbReference type="OMA" id="YYFYVGS"/>
    <dbReference type="OrthoDB" id="124734at2157"/>
    <dbReference type="PhylomeDB" id="Q58030"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
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        <property type="term" value="F:metal ion binding"/>
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    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:base-excision repair, AP site formation"/>
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    <dbReference type="GO" id="GO:0006296">
        <property type="term" value="P:nucleotide-excision repair, DNA incision, 5'-to lesion"/>
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    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
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        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50164">
        <property type="entry name" value="GIY_YIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102243" description="Endonuclease III">
        <location>
            <begin position="1"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="HhH" evidence="1">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG">
        <location>
            <begin position="229"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <sequence length="344" mass="40568" checksum="BC3BEFE1DE778B85" modified="1998-12-15" version="2">MELIEILLKKLNKNAVVTEIAKDKDPFKVLISTIISARTKDEVTEEVSKKLFKEIKDVDDLLNIDEEKLADLIYPAGFYKNKAKNLKKLAKILKENYNGKVPDSLEELLKLPGVGRKTANLVITLAFNKDGICVDTHVHRICNRWEIVDTETPEETEFELRKKLPKKYWKVINNLLVVFGREICSSKSKCDKCFKEIKEKCPYYEKIKHFENILKKFNFRKVSKNKIPNEKGTYILKIRLKEGKKIKFGKTERFFKKGYYFYIGSAFGNSMNLKNRIERHLKDDKKMHWHIDYLLKYGKIEEIYITNERVECEVANEFIKKFDFVENFGCSDCKCKSHLFYLKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58058</accession>
    <name>PGK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoglycerate kinase</fullName>
            <ecNumber>2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">pgk</name>
        <name type="ordered locus">MJ0641</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64380">
        <property type="entry name" value="A64380"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58058"/>
    <dbReference type="SMR" id="Q58058"/>
    <dbReference type="STRING" id="243232.MJ_0641"/>
    <dbReference type="EnsemblBacteria" id="AAB98636">
        <property type="protein sequence ID" value="AAB98636"/>
        <property type="gene ID" value="MJ_0641"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0641"/>
    <dbReference type="eggNOG" id="arCOG00496">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025427_0_2_2"/>
    <dbReference type="InParanoid" id="Q58058"/>
    <dbReference type="OMA" id="DMIFDIG"/>
    <dbReference type="PhylomeDB" id="Q58058"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015911">
        <property type="entry name" value="Phosphoglycerate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PTHR11406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="SSF53748"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00111">
        <property type="entry name" value="PGLYCERATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000146058" description="Phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="62"/>
            <end position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="366"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="417" mass="46133" checksum="C87589CCCE5835E0" modified="1996-11-01" version="1">MIMFLTLDDFNFEDKRVVLRVDINCPIDPNTGEILDDKRIREIKSTITELINKGAKVVILAHQSRPGKKDFTTLKNHAKVLSDVIGKEVEYIDEVIGSTAREAIINMKCGDVILLENVRFYSEEVLSDWKKWENITPKKQAETNLIKRLAPLFDYFVNDAFAAAHRAQPSLVGFSYYMPMIAGRLMEREVGVLSKVLENPEKPCVYVLGGAKADDSIRVMKNVLENGTADKVLTSGIVANIFLVAMGYDLGVNMDIIENLGLKSQIEIAKELLNKFEDKIVVPVDVALNINEERVEADLNKDEKVEHLINDIGEKTIELYSEIINEAKTIVANGPAGVFEKEAFAKGTEELLKAIANSKGFSVIGGGHLSAAAELFGIADKIDHVSTGGGATLDFLAGEKLPVIEMLKESYKKYKGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q58130</accession>
    <name>LEU3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>3-isopropylmalate/3-methylmalate dehydrogenase</fullName>
            <ecNumber>1.1.1.85</ecNumber>
            <ecNumber>1.1.1.n5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>3-isopropylmalate dehydrogenase</fullName>
            <shortName>3-IPM-DH</shortName>
            <shortName>IMDH</shortName>
            <shortName>IPMDH</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Beta-IPM dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>D-malate dehydrogenase [decarboxylating]</fullName>
            <ecNumber>1.1.1.83</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuB</name>
        <name type="ordered locus">MJ0720</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="5013" last="5016">
            <title>Identification of enzymes homologous to isocitrate dehydrogenase that are involved in coenzyme B and leucine biosynthesis in methanoarchaea.</title>
            <authorList>
                <person name="Howell D.M."/>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10940051"/>
            <dbReference type="DOI" id="10.1128/JB.182.17.5013-5016.2000"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2007" name="J. Bacteriol." volume="189" first="4391" last="4400">
            <title>Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Drevland R.M."/>
                <person name="Waheed A."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="17449626"/>
            <dbReference type="DOI" id="10.1128/JB.00166-07"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate, which decarboxylates to 4-methyl-2-oxopentanoate (2-oxoisocaproate). Also catalyzes the oxidative decarboxylation of 3-methylmalate to 2-oxobutyrate, and that of D-malate to pyruvate. Cannot use NADP(+) instead of NAD(+). Cannot catalyze the oxidation of L-malate, L-tartrate, D-tartrate, DL-isocitrate, or DL-lactate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32271"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.85"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R,3S)-3-methylmalate + NAD(+) = 2-oxobutanoate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:32715"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16763"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58511"/>
            <dbReference type="EC" id="1.1.1.n5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-malate + NAD(+) = CO2 + NADH + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:18365"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.83"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) or Mn(2+) ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">24 uM for 3-isopropylmalate</KM>
            <KM evidence="2">410 uM for D-malate</KM>
        </kinetics>
        <temperatureDependence>
            <text>Loses 50% of its activity after heating at 80 degrees Celsius for 10 min.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85"/>
    <dbReference type="EC" id="1.1.1.n5"/>
    <dbReference type="EC" id="1.1.1.83"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870225.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58130"/>
    <dbReference type="SMR" id="Q58130"/>
    <dbReference type="STRING" id="243232.MJ_0720"/>
    <dbReference type="EnsemblBacteria" id="AAB98716">
        <property type="protein sequence ID" value="AAB98716"/>
        <property type="gene ID" value="MJ_0720"/>
    </dbReference>
    <dbReference type="GeneID" id="1451597"/>
    <dbReference type="KEGG" id="mja:MJ_0720"/>
    <dbReference type="eggNOG" id="arCOG01163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="InParanoid" id="Q58130"/>
    <dbReference type="OMA" id="TCAHKAN"/>
    <dbReference type="OrthoDB" id="33452at2157"/>
    <dbReference type="PhylomeDB" id="Q58130"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-13649"/>
    <dbReference type="SABIO-RK" id="Q58130"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00069"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00072"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046553">
        <property type="term" value="F:D-malate dehydrogenase (decarboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000083809" description="3-isopropylmalate/3-methylmalate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="260"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="1">
        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="site" description="Important for catalysis" evidence="1">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17449626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="333" mass="36330" checksum="0618F119A4E682F0" modified="1998-12-15" version="2">MHKICVIEGDGIGKEVVPATIQVLEATGLPFEFVYAEAGDEVYKRTGKALPEETIETALDCDAVLFGAAGETAADVIVKLRHILDTYANIRPVKAYKGVKCLRPDIDYVIVRENTEGLYKGIEAEIDEGITIATRVITEKACERIFRFAFNLARERKKMGKEGKVTCAHKANVLKLTDGLFKKIFYKVAEEYDDIKAEDYYIDAMNMYIITKPQVFDVVVTSNLFGDILSDGAAGTVGGLGLAPSANIGDEHGLFEPVHGSAPDIAGKKIANPTATILSAVLMLRYLGEYEAADKVEKALEEVLALGLTTPDLGGNLNTFEMAEEVAKRVREE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58142</accession>
    <name>Y732_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0732</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0732</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64391">
        <property type="entry name" value="D64391"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58142"/>
    <dbReference type="SMR" id="Q58142"/>
    <dbReference type="STRING" id="243232.MJ_0732"/>
    <dbReference type="EnsemblBacteria" id="AAB98728">
        <property type="protein sequence ID" value="AAB98728"/>
        <property type="gene ID" value="MJ_0732"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0732"/>
    <dbReference type="eggNOG" id="arCOG00509">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017490_0_0_2"/>
    <dbReference type="InParanoid" id="Q58142"/>
    <dbReference type="OMA" id="HVKNNIH"/>
    <dbReference type="PhylomeDB" id="Q58142"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107007" description="Uncharacterized protein MJ0732">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="250"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00088"/>
        </source>
    </evidence>
    <sequence length="393" mass="45555" checksum="CF01B59793BBA20F" modified="1996-11-01" version="1">MILMVLEIKNGIYWVGVIDWEIRDFHGYGTPYGSTYNSYLIKDKKNVLIDTAKDYMFNELIYGISKFIDPKDLDYIIVNHVEKDHSGCVDKLVEISNATIITNEKGKEHLSLYYDTKDWDFIIVDTGDEINIGDRTLKFIRTPMLHWPDNMLTYCKEEKILFSNDAFGQHIASSERFDYEIGEGIFEHAKDYFANILMPYKMLIPDAIKAVKNLDIELICPSHGVIWKEYINEIIEKYNEWAMNKTKNKAVIVYDTMYNSTKKMAHAIAEGLMEKGVEVKIYRVCETSLSRIMTEILDAKYVLVGSPTVNRNLYPEVGKFLAYMDCIRPLDKIGVAFGSYGWMECATEKIKEIFKNLGFKIVDDECLTVRFAPKEEHLKKCYEFGKRLADIGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58178</accession>
    <name>COFE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Coenzyme F420:L-glutamate ligase</fullName>
            <ecNumber>6.3.2.31</ecNumber>
            <ecNumber>6.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme F420-0:L-glutamate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Coenzyme F420-1:gamma-L-glutamate ligase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>F420:glutamyl ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cofE</name>
        <name type="ordered locus">MJ0768</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Biochemistry" volume="42" first="9771" last="9778">
            <title>CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii.</title>
            <authorList>
                <person name="Li H."/>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="12911320"/>
            <dbReference type="DOI" id="10.1021/bi034779b"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>REACTION MECHANISM</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the GTP-dependent successive addition of two L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form coenzyme F420-0-glutamyl-glutamate (F420-2), with a gamma-linkage between the two glutamates. Cannot use F420-2 as substrate to add more glutamates. Exhibits maximum activity with GTP, compared with UTP (66%) and dGTP (25%); with ATP, only F420-1 is observed as the product; CTP and TTP support no activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GTP + L-glutamate + oxidized coenzyme F420-0 = GDP + H(+) + oxidized coenzyme F420-1 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GTP + L-glutamate + oxidized coenzyme F420-1 = GDP + H(+) + oxidized coenzyme F420-2 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57922"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:59920"/>
            <dbReference type="EC" id="6.3.2.34"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Binds 2 divalent metal cations per subunit. The ions could be magnesium and/or manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="2">Monovalent cation. The ion could be potassium.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">1 uM for F420-0</KM>
            <KM evidence="2">0.21 uM for F420-1</KM>
            <Vmax evidence="2">2.4 nmol/min/mg enzyme with F420-0 as substrate</Vmax>
            <Vmax evidence="2">0.96 nmol/min/mg enzyme with F420-1 as substrate</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 8.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 60 degrees Celsius. Thermostable. Retains 70% of its activity after heating at 80 degrees Celsius for 15 minutes.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CofE family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.31"/>
    <dbReference type="EC" id="6.3.2.34"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64395">
        <property type="entry name" value="H64395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870273.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58178"/>
    <dbReference type="SMR" id="Q58178"/>
    <dbReference type="STRING" id="243232.MJ_0768"/>
    <dbReference type="EnsemblBacteria" id="AAB98763">
        <property type="protein sequence ID" value="AAB98763"/>
        <property type="gene ID" value="MJ_0768"/>
    </dbReference>
    <dbReference type="GeneID" id="1451645"/>
    <dbReference type="KEGG" id="mja:MJ_0768"/>
    <dbReference type="eggNOG" id="arCOG02714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051152_1_1_2"/>
    <dbReference type="InParanoid" id="Q58178"/>
    <dbReference type="OMA" id="KHGFVCA"/>
    <dbReference type="OrthoDB" id="73785at2157"/>
    <dbReference type="PhylomeDB" id="Q58178"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-12187"/>
    <dbReference type="BRENDA" id="6.3.2.31">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="6.3.2.34">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58178"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052619">
        <property type="term" value="F:coenzyme F420-1:gamma-L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01258">
        <property type="entry name" value="F420_ligase_CofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008225">
        <property type="entry name" value="F420-0_g-glutamyl_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023659">
        <property type="entry name" value="F420_ligase_CofE_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01916">
        <property type="entry name" value="F420_cofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145789" description="Coenzyme F420:L-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="211"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12911320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="249" mass="27148" checksum="A6B66FF4B52C15A2" modified="1996-11-01" version="1">MIKEKRKVEVIGLELPIFKGGEQINLSELIAQYPIEDGDIIVIAETLISKLEGGVIDRDKIIPSKEAIELAKKTGKDPKVVQVILDEAKEIVKVGKNFIITETKHGFVCANSGVDESNIYKGIKILPKNPDESAEKIRKEIEKLTGKRVGVIISDSVGRPFRKGAVGIAIGVSGILALWDRKGEKDLFGRELKTTEVAIADELASMANVVMGEADEGIPVVIIRGANVPFGNGKGRDLIRPKEEDVFRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58184</accession>
    <name>Y774_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0774</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0774</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64396">
        <property type="entry name" value="F64396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870279.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58184"/>
    <dbReference type="SMR" id="Q58184"/>
    <dbReference type="STRING" id="243232.MJ_0774"/>
    <dbReference type="EnsemblBacteria" id="AAB98778">
        <property type="protein sequence ID" value="AAB98778"/>
        <property type="gene ID" value="MJ_0774"/>
    </dbReference>
    <dbReference type="GeneID" id="1451651"/>
    <dbReference type="KEGG" id="mja:MJ_0774"/>
    <dbReference type="eggNOG" id="arCOG00472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_670165_0_0_2"/>
    <dbReference type="InParanoid" id="Q58184"/>
    <dbReference type="OMA" id="LHYEFKL"/>
    <dbReference type="OrthoDB" id="15005at2157"/>
    <dbReference type="PhylomeDB" id="Q58184"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF46785"/>
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        <property type="entry name" value="SSF52540"/>
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        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000160633" description="Uncharacterized HTH-type transcriptional regulator MJ0774">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="305"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00340"/>
        </source>
    </evidence>
    <sequence length="409" mass="46329" checksum="34AF708F87D7BE43" modified="1996-11-01" version="1">MDPIEFIQKSASSIASTMHKLKLKYNPFSEKPIRGNTKFFVGRVSELREIGEILGSALHGSVANAAIVGTKGIGKSSMLNIIYYATKKQGHWVVEMTASQVTPRQFLIQLLYNILTENIITMSGTIKSDYMEHSSRILDMYRKLNNYGDKVPIHYPRERIERDLEYLINEVKSPDKLCIILIDEADQFAKKSCLSLLQFFHSFLYEEGILTFMAGSPTLMDDLTKISPPIKDRIPKIINMPPLSKDESYDLIRRRLEDAHIDGAEEFEPFTEEAIHKIVEECDGIPRKIIMTCSESISIAIRKGLTKIDEKVVKEAMHSLGISVGHQILNHLTPAQSEIVRAMAELGGSATVTQLAEYLKKSPSTIGTHLSDIYEMGYIYKEREGNNVYYKLSKELRDVLIGEDDELEM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q58194</accession>
    <name>HMD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>5,10-methenyltetrahydromethanopterin hydrogenase</fullName>
            <ecNumber>1.12.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>H(2)-dependent methylene-H(4)MPT dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>N(5),N(10)-methenyltetrahydromethanopterin hydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hmd</name>
        <name type="ordered locus">MJ0784</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="1.12.98.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (hydrogen route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMD family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64397">
        <property type="entry name" value="H64397"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A=1-358"/>
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        <property type="resolution" value="1.95 A"/>
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        <property type="resolution" value="1.75 A"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
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    <dbReference type="EnsemblBacteria" id="AAB98781">
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        <property type="gene ID" value="MJ_0784"/>
    </dbReference>
    <dbReference type="GeneID" id="1451662"/>
    <dbReference type="KEGG" id="mja:MJ_0784"/>
    <dbReference type="eggNOG" id="arCOG03196">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_772960_0_0_2"/>
    <dbReference type="OMA" id="HACTIPT"/>
    <dbReference type="OrthoDB" id="31540at2157"/>
    <dbReference type="PhylomeDB" id="Q58194"/>
    <dbReference type="BRENDA" id="1.12.98.2">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00696"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58194"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047068">
        <property type="term" value="F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01090">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
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    <dbReference type="InterPro" id="IPR010062">
        <property type="entry name" value="HMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004889">
        <property type="entry name" value="HMD_C"/>
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    <dbReference type="InterPro" id="IPR038182">
        <property type="entry name" value="HMD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024190">
        <property type="entry name" value="METHMP_Hmd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03201">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500165">
        <property type="entry name" value="HMDI"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01723">
        <property type="entry name" value="hmd_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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    <feature type="helix" evidence="3">
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    <feature type="helix" evidence="3">
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    <feature type="helix" evidence="3">
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    <feature type="helix" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="311"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="318"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="324"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2B0J"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3DAF"/>
        </source>
    </evidence>
    <sequence length="358" mass="38689" checksum="9D8E789759BCF43A" modified="1996-11-01" version="1">MKIAILGAGCYRTHAAAGITNFMRACEVAKEVGKPEIALTHSSITYGAELLHLVPDVKEVIVSDPCFAEEPGLVVIDEFDPKEVMEAHLSGNPESIMPKIREVVKAKAKELPKPPKACIHLVHPEDVGLKVTSDDREAVEGADIVITWLPKGNKQPDIIKKFADAIPEGAIVTHACTIPTTKFAKIFKDLGREDLNITSYHPGCVPEMKGQVYIAEGYASEEAVNKLYEIGKIARGKAFKMPANLIGPVCDMCSAVTATVYAGLLAYRDAVTKILGAPADFAQMMADEALTQIHNLMKEKGIANMEEALDPAALLGTADSMCFGPLAEILPTALKVLEKHKVVEEEGKTKCEIMSQKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q58234</accession>
    <name>MOAA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP 3',8-cyclase</fullName>
            <ecNumber evidence="1">4.1.99.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaA</name>
        <name type="ordered locus">MJ0824</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:49576"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.1.99.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. MoaA family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.22" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64402">
        <property type="entry name" value="H64402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870335.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58234"/>
    <dbReference type="SMR" id="Q58234"/>
    <dbReference type="STRING" id="243232.MJ_0824"/>
    <dbReference type="DNASU" id="1451707"/>
    <dbReference type="EnsemblBacteria" id="AAB98823">
        <property type="protein sequence ID" value="AAB98823"/>
        <property type="gene ID" value="MJ_0824"/>
    </dbReference>
    <dbReference type="GeneID" id="1451707"/>
    <dbReference type="KEGG" id="mja:MJ_0824"/>
    <dbReference type="eggNOG" id="arCOG00930">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009273_0_1_2"/>
    <dbReference type="InParanoid" id="Q58234"/>
    <dbReference type="OMA" id="IEFMPIG"/>
    <dbReference type="OrthoDB" id="18533at2157"/>
    <dbReference type="PhylomeDB" id="Q58234"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019008">
        <property type="term" value="C:molybdopterin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01225_A">
        <property type="entry name" value="MoaA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="Mob_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000153017" description="Probable GTP 3',8-cyclase">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="4"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="248"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-substrate</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="298" mass="34652" checksum="EA92B9A0A75BFD55" modified="1996-11-01" version="1">MKDKFGREIRSLRISITNKCNLQCFYCHREGHDSNNDRYMTPEEIGIIAKTSTKFGVKKIKISGGEPLLRKDVCEIIENIKDERIKDISLTTNGILLENLAEKLKDAGLNRVNVSLDTLNPELYKKITKFGDVERVINGIKKAIDVSLTPLKVNFLAMSINIKDLPDIMEFCRDIGAILQIIEFIPLKEELKGYYYNISPIENEIKEKADKVITRNFMQNRKKYIVDGLEIEFVRPMDNSEFCMHCTRIRLTYDGYLKPCLLRDDNLVDVLTPLRKGENLEPYFIECINRREPYFKIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58257</accession>
    <name>MTRE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit E</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrE</name>
        <name type="ordered locus">MJ0847</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrE family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64405">
        <property type="entry name" value="G64405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870361.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58257"/>
    <dbReference type="STRING" id="243232.MJ_0847"/>
    <dbReference type="EnsemblBacteria" id="AAB98852">
        <property type="protein sequence ID" value="AAB98852"/>
        <property type="gene ID" value="MJ_0847"/>
    </dbReference>
    <dbReference type="GeneID" id="1451735"/>
    <dbReference type="KEGG" id="mja:MJ_0847"/>
    <dbReference type="eggNOG" id="arCOG04870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_958513_0_0_2"/>
    <dbReference type="InParanoid" id="Q58257"/>
    <dbReference type="OMA" id="QMGNIHR"/>
    <dbReference type="OrthoDB" id="50292at2157"/>
    <dbReference type="PhylomeDB" id="Q58257"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01098">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005780">
        <property type="entry name" value="MeTrfase_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04206">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016509">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01113">
        <property type="entry name" value="mtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147539" description="Tetrahydromethanopterin S-methyltransferase subunit E">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="303" mass="31635" checksum="46BA1BBD80FECCE2" modified="1997-11-01" version="1">MDATLIALGALALSGALATVAGCAEDLESDVGSQSNPNSQVQLAPQMGNIHRYFNKAISGEPVSYGLYVAVAGTVAYAIMQMGLNPILALILGAGVAAFVHGAYAISAYLGRIVGQSKNFGQPVYWDVVMSHLGPIVGHGFIAVFCMVLMAYLANTILGNPFPLPLIALIFGITVGAIGSSTGDVHYGAEREYQKYPFGGGVPVANHGDIDIKAEYGLRNGMDSSYFCSRLGGVLTGLCFGLIVFLDGWRGVLGDILKGGQGGSVITASIISIVIGLIIVAILAIINRKVEVFARNKYGPYTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58292</accession>
    <name>Y882_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable S-adenosylmethionine-dependent methyltransferase MJ0882</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0882</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Struct. Funct. Genomics" volume="2" first="121" last="127">
            <title>Structure-based experimental confirmation of biochemical function to a methyltransferase, MJ0882, from hyperthermophile Methanococcus jannaschii.</title>
            <authorList>
                <person name="Huang L."/>
                <person name="Hung L."/>
                <person name="Odell M."/>
                <person name="Yokota H."/>
                <person name="Kim R."/>
                <person name="Kim S.H."/>
            </authorList>
            <dbReference type="PubMed" id="12836702"/>
            <dbReference type="DOI" id="10.1023/A:1021279113558"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 4-197</scope>
        <scope>S-ADENOSYLMETHIONINE BINDING</scope>
    </reference>
    <comment type="function">
        <text>Probable methyltransferase that uses S-adenosylmethionine as the methyl donor. Binds neither NAD nor NADP in vitro.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64410">
        <property type="entry name" value="B64410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870396.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1DUS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=4-197"/>
    </dbReference>
    <dbReference type="PDBsum" id="1DUS"/>
    <dbReference type="AlphaFoldDB" id="Q58292"/>
    <dbReference type="SMR" id="Q58292"/>
    <dbReference type="STRING" id="243232.MJ_0882"/>
    <dbReference type="EnsemblBacteria" id="AAB98886">
        <property type="protein sequence ID" value="AAB98886"/>
        <property type="gene ID" value="MJ_0882"/>
    </dbReference>
    <dbReference type="GeneID" id="1451771"/>
    <dbReference type="KEGG" id="mja:MJ_0882"/>
    <dbReference type="eggNOG" id="arCOG00110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_7_2_2"/>
    <dbReference type="InParanoid" id="Q58292"/>
    <dbReference type="OMA" id="HMIDVNE"/>
    <dbReference type="OrthoDB" id="72076at2157"/>
    <dbReference type="PhylomeDB" id="Q58292"/>
    <dbReference type="EvolutionaryTrace" id="Q58292"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071704">
        <property type="term" value="P:organic substance metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000157176" description="Probable S-adenosylmethionine-dependent methyltransferase MJ0882">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="35"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="42"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="58"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="68"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="74"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="77"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="107"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="114"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="123"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="136"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="150"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="164"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="181"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="189"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1DUS"/>
        </source>
    </evidence>
    <sequence length="197" mass="22244" checksum="F4E108E500ABEB28" modified="1996-11-01" version="1">MHYFSEKPTTKSDVKIVEDILRGKKLKFKTDSGVFSYGKVDKGTKILVENVVVDKDDDILDLGCGYGVIGIALADEVKSTTMADINRRAIKLAKENIKLNNLDNYDIRVVHSDLYENVKDRKYNKIITNPPIRAGKEVLHRIIEEGKELLKDNGEIWVVIQTKQGAKSLAKYMKDVFGNVETVTIKGGYRVLKSKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58326</accession>
    <name>Y916_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0916</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0916</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64414">
        <property type="entry name" value="D64414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870430.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58326"/>
    <dbReference type="SMR" id="Q58326"/>
    <dbReference type="STRING" id="243232.MJ_0916"/>
    <dbReference type="EnsemblBacteria" id="AAB98921">
        <property type="protein sequence ID" value="AAB98921"/>
        <property type="gene ID" value="MJ_0916"/>
    </dbReference>
    <dbReference type="GeneID" id="1451805"/>
    <dbReference type="KEGG" id="mja:MJ_0916"/>
    <dbReference type="eggNOG" id="arCOG02155">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170070_0_0_2"/>
    <dbReference type="InParanoid" id="Q58326"/>
    <dbReference type="OMA" id="YTTTAIY"/>
    <dbReference type="OrthoDB" id="113119at2157"/>
    <dbReference type="PhylomeDB" id="Q58326"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="SSF50346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107103" description="Uncharacterized protein MJ0916">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <sequence length="89" mass="9673" checksum="ADA40C45D1D858F9" modified="1998-12-15" version="2">MAIRVSDILDKPIYTTTAIYVGKVYDVMLDLNKGVISGLIVSDIQNGCLKDYVTDPSKKVVLPFNLITAIGNIILVKPPADSGYGFLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58345</accession>
    <name>Y935_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0935</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0935</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64416">
        <property type="entry name" value="G64416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870449.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58345"/>
    <dbReference type="STRING" id="243232.MJ_0935"/>
    <dbReference type="EnsemblBacteria" id="AAB98940">
        <property type="protein sequence ID" value="AAB98940"/>
        <property type="gene ID" value="MJ_0935"/>
    </dbReference>
    <dbReference type="GeneID" id="1451831"/>
    <dbReference type="KEGG" id="mja:MJ_0935"/>
    <dbReference type="eggNOG" id="arCOG03229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_102155_0_0_2"/>
    <dbReference type="InParanoid" id="Q58345"/>
    <dbReference type="OMA" id="FVANHYK"/>
    <dbReference type="OrthoDB" id="73889at2157"/>
    <dbReference type="PhylomeDB" id="Q58345"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007368">
        <property type="entry name" value="DUF434"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041652">
        <property type="entry name" value="DUF5616"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04256">
        <property type="entry name" value="DUF434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18481">
        <property type="entry name" value="DUF5616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107112" description="Uncharacterized protein MJ0935">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <sequence length="208" mass="24162" checksum="B2F3D0027B0C1B6D" modified="1996-11-01" version="1">MSIEKAKEDFKYLINRGYKKDVALNFVANHYKLSKEDRLKIIRTTHSDKEIKLTKRKLKKLKDFKGKTIYIDGFNVLISLEALIKGNKIVLCDDNIYRDFENVYGKYKINEYSDKAISLLLEILKHYNIKAVIYLDAQVSKSGILAKKIREKMKAFSIEGEVFCVKNCDYELKNKEVVATSDSVIIKSENVKYVVDLIAEAIEYLKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58354</accession>
    <name>Y944_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0944</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0944</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0361.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64417">
        <property type="entry name" value="H64417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870458.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58354"/>
    <dbReference type="SMR" id="Q58354"/>
    <dbReference type="EnsemblBacteria" id="AAB98954">
        <property type="protein sequence ID" value="AAB98954"/>
        <property type="gene ID" value="MJ_0944"/>
    </dbReference>
    <dbReference type="GeneID" id="1451841"/>
    <dbReference type="KEGG" id="mja:MJ_0944"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153628_2_0_2"/>
    <dbReference type="InParanoid" id="Q58354"/>
    <dbReference type="OMA" id="HNIINAK"/>
    <dbReference type="OrthoDB" id="130706at2157"/>
    <dbReference type="PhylomeDB" id="Q58354"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005149">
        <property type="entry name" value="Tscrpt_reg_PadR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03551">
        <property type="entry name" value="PadR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107115" description="Uncharacterized protein MJ0944">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="120" mass="13862" checksum="3219CC06451A7B49" modified="1996-11-01" version="1">MLIGILSKKYVKEILELLNEKGELHFSQIHKEIPTHMSSLNRTLNELVKLGLISKRKEDNKQALPKTYYKLTPLGKKALLLYEVEKIIENSKNNQNIIIQIINGKNHNIINAKIVNIHNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q58386</accession>
    <name>Y976_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0976</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0976</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64421">
        <property type="entry name" value="H64421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870490.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58386"/>
    <dbReference type="SMR" id="Q58386"/>
    <dbReference type="STRING" id="243232.MJ_0976"/>
    <dbReference type="EnsemblBacteria" id="AAB98982">
        <property type="protein sequence ID" value="AAB98982"/>
        <property type="gene ID" value="MJ_0976"/>
    </dbReference>
    <dbReference type="GeneID" id="1451874"/>
    <dbReference type="KEGG" id="mja:MJ_0976"/>
    <dbReference type="HOGENOM" id="CLU_755685_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107126" description="Uncharacterized protein MJ0976">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="366" mass="42587" checksum="59302DEF70EEE594" modified="1996-11-01" version="1">MSEDSNPKNDLKLKMYETFRSHIERAENSFWTYMLFYLQFLVGINGAVGILCRYLSNNLQNFPTEIIIAFLALVNILLSLIGIIVIIERGKWFFRNMILTVNLERYILKEEHIKIIPKRYSKFDNFNKVIDTTGRIFISIFIGIYMISVIALGYLANSCKTIILFGTGFFIIVVIIYFLDLLDWIYRRIDSNEYRKVIGVVLFISFIPPLMNYFIYDIINLICSYIPESFIVAMIVILIFVIIDVYYNAKKHIENTIIMLSLERTVNDIDDIIKILKNIRLDDTKKEELKKKLRKIKKLIENVIKLLGGTSENGTQNNNLEEAKSKITEACRKISGNNIFEAINELNEAKYIINNKINELTQSDSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58395</accession>
    <name>Y988_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0988</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0988</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64423">
        <property type="entry name" value="D64423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870502.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58395"/>
    <dbReference type="SMR" id="Q58395"/>
    <dbReference type="STRING" id="243232.MJ_0988"/>
    <dbReference type="EnsemblBacteria" id="AAB98990">
        <property type="protein sequence ID" value="AAB98990"/>
        <property type="gene ID" value="MJ_0988"/>
    </dbReference>
    <dbReference type="GeneID" id="1451886"/>
    <dbReference type="KEGG" id="mja:MJ_0988"/>
    <dbReference type="eggNOG" id="arCOG01565">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070736_0_0_2"/>
    <dbReference type="InParanoid" id="Q58395"/>
    <dbReference type="OMA" id="CHHNADP"/>
    <dbReference type="OrthoDB" id="47681at2157"/>
    <dbReference type="PhylomeDB" id="Q58395"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107131" description="Uncharacterized protein MJ0988">
        <location>
            <begin position="1"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="329" mass="37316" checksum="7AE87D2F92E535E4" modified="1996-11-01" version="1">MELLEYLKRDEVLFLCHHNADPDAVGSCVALKYLASQLNPNGKFRISADSVSKLSRNILNEIGERVDIEIYPKLPETVFIVDTASINQLKVNFDELKEREVILIDHHKKTDLADICKYYIIKEDYPSTSEIIAEIFKELNIFPPKNVRIALLCGIVYDTKHLKLANSKTFELISYLIKDISFQKILYLLSQESDVSKRTAHLKACSRMEIREFDKLRIALSHVSSHEASCAKTIVSIGADVAFVVAVRKKEKEIRVSARCRKHVSKYVHLGNLMEKIGKELGGSGGGHSEAGGLNAPYDKSKSKEKVIKEVLNLCYKRFVEEYKKAKQN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58430</accession>
    <name>Y1024_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1024</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1024</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To B.subtilis YhaP.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64427">
        <property type="entry name" value="G64427"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58430"/>
    <dbReference type="STRING" id="243232.MJ_1024"/>
    <dbReference type="EnsemblBacteria" id="AAB99028">
        <property type="protein sequence ID" value="AAB99028"/>
        <property type="gene ID" value="MJ_1024"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1024"/>
    <dbReference type="eggNOG" id="arCOG01462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046841_0_1_2"/>
    <dbReference type="InParanoid" id="Q58430"/>
    <dbReference type="OMA" id="FGYLIMM"/>
    <dbReference type="PhylomeDB" id="Q58430"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107147" description="Uncharacterized protein MJ1024">
        <location>
            <begin position="1"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="403" mass="45294" checksum="6242E4EF26F7191C" modified="1996-11-01" version="1">MNSGDIMKLNIKKILTIGKREVLSNIKRKQFLIATIIGPLIIIALAIIGSFMMFDIKEIKVGYVDEFGLGIPNKVVENNFGKNTTIYFIKYENIEKGKEDVLNKSIDALIVIPKDYLDSGKIILYSTTKSPNPIITDTLNKFLLKKLLKGKVDNKTYNRVINPMNLEIYSVSKKGFEKETFLSQLLPIGFVFLLYMAISSLSGIIVSSIIEEKQNRIMELLLCYSSAENLMFGKILGISAVGLLQIGIWVLFALPIIITYAVKVSLYLAIFALIYFVLGYLFYSSLLCGLSSLFSHPKDASQLISPIIIIQIIPIMFMNTIMVNPNHYMAKILSYVPFTAPYAVVLRASVTQLPLIEIVLSTAIMIVSIVISFILSIKLFKIGVLLYEENLTLKRVIKIIFKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58466</accession>
    <name>Y1066_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1066</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1066</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64433">
        <property type="entry name" value="A64433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870579.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58466"/>
    <dbReference type="SMR" id="Q58466"/>
    <dbReference type="STRING" id="243232.MJ_1066"/>
    <dbReference type="EnsemblBacteria" id="AAB99069">
        <property type="protein sequence ID" value="AAB99069"/>
        <property type="gene ID" value="MJ_1066"/>
    </dbReference>
    <dbReference type="GeneID" id="1451963"/>
    <dbReference type="KEGG" id="mja:MJ_1066"/>
    <dbReference type="eggNOG" id="arCOG00118">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033332_7_2_2"/>
    <dbReference type="InParanoid" id="Q58466"/>
    <dbReference type="OMA" id="PGDAVFC"/>
    <dbReference type="OrthoDB" id="37018at2157"/>
    <dbReference type="PhylomeDB" id="Q58466"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020026">
        <property type="entry name" value="PseC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="PTHR30244"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03588">
        <property type="entry name" value="PseC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000110032" description="Uncharacterized protein MJ1066">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="386" mass="44501" checksum="FE8F43C8D50CCB80" modified="1996-11-01" version="1">MIRESFLPPFRPCIGEEEINEVIDTLKSDWITMGPKTLKFEELFRNYIGSKFAISLNSCTAGLHLSLVALNIKDKDEVITTPYTFAATGNVIVHQRAKPVFVDIDKETYNINVEEIENAITERTKAIIPVHYAGHPCEMDEILKIARDYDLYVIEDAAHALGAEYKGKKIGTIGDTTSFSFYATKNITTGEGGMVTTDNEEIAEKIKILRLHGISRDAWKRYSSEGSWYYEIIECGYKYNMTDIQASIGIHQLKKAEIMRKRREEIAKIYNEEFENLEGLITPTIKKHVKHAWHLYPLLINIDRLKINRTKFIEELKKQNIGTSVHFIPLHLHPFYRKTFGYKKGDFPNAEWVYEREISLPIYPKMTDDDVIDVVNAVKKIVSENR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58477</accession>
    <name>SYS2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Type-2 serine--tRNA ligase</fullName>
            <ecNumber>6.1.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Seryl-tRNA synthetase</fullName>
            <shortName>SerRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Seryl-tRNA(Ser/Sec) synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">serS</name>
        <name type="ordered locus">MJ1077</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Eur. J. Biochem." volume="271" first="694" last="702">
            <title>The unusual methanogenic seryl-tRNA synthetase recognizes tRNASer species from all three kingdoms of life.</title>
            <authorList>
                <person name="Bilokapic S."/>
                <person name="Korencic D."/>
                <person name="Soell D."/>
                <person name="Weygand-Durasevic I."/>
            </authorList>
            <dbReference type="PubMed" id="14764085"/>
            <dbReference type="DOI" id="10.1111/j.1432-1033.2003.03971.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>TEMPERATURE DEPENDENCE</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-serine + tRNA(Ser) = AMP + diphosphate + H(+) + L-seryl-tRNA(Ser)</text>
            <dbReference type="Rhea" id="RHEA:12292"/>
            <dbReference type="Rhea" id="RHEA-COMP:9669"/>
            <dbReference type="Rhea" id="RHEA-COMP:9703"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-serine + tRNA(Sec) = AMP + diphosphate + H(+) + L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:42580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:10128"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 Zn(2+) ion per subunit. This ion is coordinated with 2 cysteines, 1 glutamate and a water molecule that dissociates from the zinc ion to allow the coordination of the amino group of the serine substrate, which is essential for catalysis.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="2">Thermostable. Fully active at 80 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of two distinct domains, a catalytic core and a N-terminal extension that is presumably involved in tRNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Type-2 seryl-tRNA synthetase subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64434">
        <property type="entry name" value="D64434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870589.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58477"/>
    <dbReference type="SMR" id="Q58477"/>
    <dbReference type="STRING" id="243232.MJ_1077"/>
    <dbReference type="PRIDE" id="Q58477"/>
    <dbReference type="EnsemblBacteria" id="AAB99075">
        <property type="protein sequence ID" value="AAB99075"/>
        <property type="gene ID" value="MJ_1077"/>
    </dbReference>
    <dbReference type="GeneID" id="1451973"/>
    <dbReference type="KEGG" id="mja:MJ_1077"/>
    <dbReference type="eggNOG" id="arCOG00403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_542524_0_0_2"/>
    <dbReference type="InParanoid" id="Q58477"/>
    <dbReference type="OMA" id="YYVCPPK"/>
    <dbReference type="OrthoDB" id="9543at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00895"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006434">
        <property type="term" value="P:seryl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01278">
        <property type="entry name" value="Ser_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004503">
        <property type="entry name" value="Ser-tRNA-ligase_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041293">
        <property type="entry name" value="SerS_tRNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18490">
        <property type="entry name" value="tRNA_bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00415">
        <property type="entry name" value="serS_MJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000122174" description="Type-2 serine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="347"/>
            <end position="349"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="358"/>
            <end position="359"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="364"/>
            <end position="366"/>
        </location>
        <ligand>
            <name>L-serine</name>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="366"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="480"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="14764085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="521" mass="60632" checksum="E8D40DB16915B4FE" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q58485</accession>
    <name>Y1085_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1085</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1085</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64435">
        <property type="entry name" value="D64435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870597.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58485"/>
    <dbReference type="STRING" id="243232.MJ_1085"/>
    <dbReference type="PRIDE" id="Q58485"/>
    <dbReference type="DNASU" id="1451981"/>
    <dbReference type="EnsemblBacteria" id="AAB99095">
        <property type="protein sequence ID" value="AAB99095"/>
        <property type="gene ID" value="MJ_1085"/>
    </dbReference>
    <dbReference type="GeneID" id="1451981"/>
    <dbReference type="KEGG" id="mja:MJ_1085"/>
    <dbReference type="eggNOG" id="arCOG06454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_537068_0_0_2"/>
    <dbReference type="OMA" id="DNGVIFY"/>
    <dbReference type="OrthoDB" id="21364at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107163" description="Uncharacterized protein MJ1085">
        <location>
            <begin position="1"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="RNase NYN" evidence="1">
        <location>
            <begin position="358"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="509" mass="59471" checksum="8ACF7C4B045D6049" modified="1996-11-01" version="1">MDNMGKDKNILKIRANLIKYIDVDEIEELPKVKVKNVEEFLEAHRVLGKHVICRYKDNLEDIFFFVDNGVIFYIPSDGFKTLEDLIEAKSLGLSAEEYYEYLEFGDINEYKKYKSSGFKSIEEYKKAKDLGFIGGLEELVKEGIAQRLDDKYIIEYLYYGILENREFSNDAELYYFAIEKGFSDFDELKNALKAGFGDANEYKDALNRGFKDAYEYNDALSKGFRDADEYKIAKAIGVNSKKELEEYMELKRICENFGLETFEEGYLLKVLMDLKIDEEITLKELYNKLKEKERLMKIKKDVLNKLANLSSPSWYSTRFTTVDDLEEYLVDSEIVSYLGEYIKEEKIFRRIYPPKPSRRIVIIDAISVLNNMHNLSPNSIENLIKKIKNAGFKNIITVMDTVTYYKIKGKDICRFLANECNIKVSKSKDEAYKLIIEYIKNFGALVISNASFKDYIIKDSKLFYKDIKEYIIPFIVENGEINLDIELLRKLYTEVVTKRIEKIKSNVVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58503</accession>
    <name>RELE3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Toxin RelE3</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>MjRelE</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Putative endoribonuclease RelE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">relE3</name>
        <name type="synonym">relE</name>
        <name type="ordered locus">MJ1103</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="J. Mol. Biol." volume="393" first="898" last="908">
            <title>Crystal structure of the antitoxin-toxin protein complex RelB-RelE from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Francuski D."/>
                <person name="Saenger W."/>
            </authorList>
            <dbReference type="PubMed" id="19712680"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2009.08.048"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) IN COMPLEX WITH RELB</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF ARG-62</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. Has RNase activity (By similarity). Is very toxic upon expression in E.coli. Its toxic activity is probably neutralized by the cognate antitoxin RelB3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms heterodimers with RelB3 and possibly a heterotetramer RelE3-RelB3(2)-RelE3 from 2 heterodimers. The heterotetramer is probably not very stable in solution.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RelE toxin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64437">
        <property type="entry name" value="F64437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870615.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3BPQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="B/D=1-88"/>
    </dbReference>
    <dbReference type="PDBsum" id="3BPQ"/>
    <dbReference type="AlphaFoldDB" id="Q58503"/>
    <dbReference type="SMR" id="Q58503"/>
    <dbReference type="STRING" id="243232.MJ_1103"/>
    <dbReference type="EnsemblBacteria" id="AAB99106">
        <property type="protein sequence ID" value="AAB99106"/>
        <property type="gene ID" value="MJ_1103"/>
    </dbReference>
    <dbReference type="GeneID" id="1452000"/>
    <dbReference type="KEGG" id="mja:MJ_1103"/>
    <dbReference type="eggNOG" id="arCOG01665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155761_5_1_2"/>
    <dbReference type="OMA" id="MKVLFAK"/>
    <dbReference type="OrthoDB" id="125851at2157"/>
    <dbReference type="EvolutionaryTrace" id="Q58503"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107168" description="Toxin RelE3">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Inactivates toxin." evidence="2">
        <original>R</original>
        <variation>K</variation>
        <variation>S</variation>
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="4"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="7"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="50"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="61"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="71"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="80"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19712680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3BPQ"/>
        </source>
    </evidence>
    <sequence length="88" mass="10562" checksum="DD58E41BCBE59F5D" modified="1996-11-01" version="1">MKVLFAKTFVKDLKHVPGHIRKRIKLIIEECQNSNSLNDLKLDIKKIKGYHNYYRIRVGNYRIGIEVNGDTIIFRRVLHRKSIYDYFP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58540</accession>
    <name>COMB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2-phosphosulfolactate phosphatase</fullName>
            <ecNumber>3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">comB</name>
        <name type="ordered locus">MJ1140</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Eur. J. Biochem." volume="268" first="5176" last="5188">
            <title>Identification of coenzyme M biosynthetic 2-phosphosulfolactate phosphatase. A member of a new class of Mg2+-dependent acid phosphatases.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="11589710"/>
            <dbReference type="DOI" id="10.1046/j.0014-2956.2001.02451.x"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Hydrolyzes both enantiomers of 2-phosphosulfolactate. Able to hydrolyze both enantiomers of 2-hydroxycarboxylic acids with pseudosymmetric centers of inversion. Specifically hydrolyzes (S)-phospholactate and (S)-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text>Inhibited by vanadate.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 5.5.</text>
        </phDependence>
        <temperatureDependence>
            <text>Optimum temperature is 75 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text>Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ComB family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99140" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.3.71"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99140.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64442">
        <property type="entry name" value="C64442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496734.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58540"/>
    <dbReference type="SMR" id="Q58540"/>
    <dbReference type="STRING" id="243232.MJ_1140"/>
    <dbReference type="DNASU" id="1452036"/>
    <dbReference type="EnsemblBacteria" id="AAB99140">
        <property type="protein sequence ID" value="AAB99140"/>
        <property type="gene ID" value="MJ_1140"/>
    </dbReference>
    <dbReference type="GeneID" id="1452036"/>
    <dbReference type="KEGG" id="mja:MJ_1140"/>
    <dbReference type="eggNOG" id="arCOG04871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070028_0_1_2"/>
    <dbReference type="InParanoid" id="Q58540"/>
    <dbReference type="OMA" id="RLFMSTT"/>
    <dbReference type="OrthoDB" id="84054at2157"/>
    <dbReference type="PhylomeDB" id="Q58540"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-2263"/>
    <dbReference type="UniPathway" id="UPA00355">
        <property type="reaction ID" value="UER00470"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00490">
        <property type="entry name" value="ComB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027639">
        <property type="entry name" value="ComB_archaeal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="PTHR37311"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="SSF142823"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00298">
        <property type="entry name" value="TIGR00298"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000081459" description="2-phosphosulfolactate phosphatase">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="224" mass="25114" checksum="B981C5B5635404E9" modified="2001-12-05" version="2">MITLCNRFTEYKCGNVAIVVDVLRASTTITTLLSFIDEVYITTSTSKKENAIYIGERKGRKIEGFDFGNSPTEILANKDIIKERYENGEKVILTTTNGTRVLKSLDAEHIFIGAIVNAKYVAKAVEDFEDVSLVPCHRENNFAIDDFIGCGVIAKYLNGEFDEFIKAALELTKHDWMSLILNSSSAENLKNLGYEKDVTFAILENSIDAVGIYKKDKSKVVRFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58581</accession>
    <name>Y1181_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1181</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1181</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64447">
        <property type="entry name" value="D64447"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58581"/>
    <dbReference type="STRING" id="243232.MJ_1181"/>
    <dbReference type="EnsemblBacteria" id="AAB99189">
        <property type="protein sequence ID" value="AAB99189"/>
        <property type="gene ID" value="MJ_1181"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1181"/>
    <dbReference type="eggNOG" id="arCOG01803">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369536_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58581"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107204" description="Uncharacterized protein MJ1181">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <sequence length="199" mass="22093" checksum="699D1EBEDE274AB2" modified="1996-11-01" version="1">MIEDALKAEGITYTEDPNNKSAMVLLVNCKGQGTMPQMLAQKQLDAVIAWEPMPEIIKNKGIGKVIAHSEDLPSATGGTWANHPCCCLAASENALKNKREAVITFAKLLKDATDEINKNRDLAVKASVRWLGTNENVEKDSIEHIKFDYRLEPVIPKVIKFVEAMKLQGLINGKLKDASSEEVKDIIFDLQTYNEIINN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58601</accession>
    <name>HIS1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>ATP phosphoribosyltransferase</fullName>
            <shortName>ATP-PRT</shortName>
            <shortName>ATP-PRTase</shortName>
            <ecNumber>2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisG</name>
        <name type="ordered locus">MJ1204</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64450">
        <property type="entry name" value="C64450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870716.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58601"/>
    <dbReference type="SMR" id="Q58601"/>
    <dbReference type="STRING" id="243232.MJ_1204"/>
    <dbReference type="PRIDE" id="Q58601"/>
    <dbReference type="EnsemblBacteria" id="AAB99208">
        <property type="protein sequence ID" value="AAB99208"/>
        <property type="gene ID" value="MJ_1204"/>
    </dbReference>
    <dbReference type="GeneID" id="1452099"/>
    <dbReference type="KEGG" id="mja:MJ_1204"/>
    <dbReference type="eggNOG" id="arCOG02208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038115_1_1_2"/>
    <dbReference type="InParanoid" id="Q58601"/>
    <dbReference type="OMA" id="YVMMDYD"/>
    <dbReference type="OrthoDB" id="56745at2157"/>
    <dbReference type="PhylomeDB" id="Q58601"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP_PRibTrfase_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="PTHR21403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000151882" description="ATP phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="288" mass="31860" checksum="121E6F9D44A61858" modified="1996-11-01" version="1">MIMFALPNKGRISEPVMKVLEKAGLKITVKGRSLFANTVDDNIKVMFARARDIPEFVADGVADIGVTGYDLVLERNVEDKVDFLLDFGFGFAKLVLAAPESSNINSIDDIKEGMRVATEFPNLTKKYFEKLNKKVEIIELSGATEIAPFIGIADLISDLTSTGTTLRLNRLKVIDEIVSSTTRLIANKNSLKDKEKREKINQIVIAIKSVLFAETKRLIMMNAPKDKVEEIRKLIPGMAGPTVSKVLSDDNMVAIHAVVNEDEIFTLVPKLHALGARDILVVPIERIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q58626</accession>
    <name>PYCA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Pyruvate carboxylase subunit A</fullName>
            <ecNumber>6.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Pyruvic carboxylase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pycA</name>
        <name type="ordered locus">MJ1229</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Arch. Microbiol." volume="174" first="406" last="414">
            <title>A stable archaeal pyruvate carboxylase from the hyperthermophile Methanococcus jannaschii.</title>
            <authorList>
                <person name="Mukhopadhyay B."/>
                <person name="Patel V.J."/>
                <person name="Wolfe R.S."/>
            </authorList>
            <dbReference type="PubMed" id="11195096"/>
            <dbReference type="DOI" id="10.1007/s002030000225"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-12</scope>
        <scope>FUNCTION</scope>
        <scope>MASS SPECTROMETRY</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + hydrogencarbonate + pyruvate = ADP + H(+) + oxaloacetate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text>Inhibited by magnesium, when its concentration exceeded the ATP one, and by high concentration of ATP and alpha-ketoglutarate.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 8.5.</text>
        </phDependence>
        <temperatureDependence>
            <text>Optimum temperature is 80-90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text>Heterooctamer of four A and four B subunits.</text>
    </comment>
    <comment type="mass spectrometry" mass="55500.0" method="MALDI" evidence="4"/>
    <dbReference type="EC" id="6.4.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64453">
        <property type="entry name" value="D64453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870741.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58626"/>
    <dbReference type="SMR" id="Q58626"/>
    <dbReference type="STRING" id="243232.MJ_1229"/>
    <dbReference type="PRIDE" id="Q58626"/>
    <dbReference type="EnsemblBacteria" id="AAB99232">
        <property type="protein sequence ID" value="AAB99232"/>
        <property type="gene ID" value="MJ_1229"/>
    </dbReference>
    <dbReference type="GeneID" id="1452125"/>
    <dbReference type="KEGG" id="mja:MJ_1229"/>
    <dbReference type="eggNOG" id="arCOG01590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_3_2_2"/>
    <dbReference type="InParanoid" id="Q58626"/>
    <dbReference type="OMA" id="FVEICSH"/>
    <dbReference type="OrthoDB" id="36803at2157"/>
    <dbReference type="PhylomeDB" id="Q58626"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146828" description="Pyruvate carboxylase subunit A">
        <location>
            <begin position="1"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin carboxylation">
        <location>
            <begin position="1"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="3">
        <location>
            <begin position="120"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="11195096"/>
        </source>
    </evidence>
    <sequence length="501" mass="55403" checksum="04D2E401892F872F" modified="1997-11-01" version="1">MFNKVLIANRGEIAIRIIRACWELGIKTVAVYSEADKRSLHATLADEAYCIGPAPAAKSYLNIDAILNVAEKAKVDAIHPGYGFLAENAEFARAVKKAGFEFIGPNPDAIEAMGSKINAKKIMKKAGVPLIPGSEGAIEDIDEAIEIAEAIGFPVVVKASAGGGGMGMSVAYSKEELKEVIESARNIAKSAFGDPTVFIEKYLENPRHIEIQLLGDKHGNIIHLGDRECSIQRRHQKLIEEAPSPIMTEELRERMGEAAIKAGKAINYDSAGTVEFLYENGNFYFLEMNTRIQVEHTVTEQVTGIDLVKAMIKIAAGEELTLKQEDVKIRGHAIECRINAEDPLNDFVPCPGKIKLYRSPGGPGVRIDSGVYGGAEIPPYYDSMIAKLITYGNSREEAIARMKRALREYVIIGVKTNIPFHRAVLEEENFLKGNISTHYVEQNMHKLREKMVKYALESRDLYSVVSEKVFEKNKKIAAAVGGLTMYISQIMKENEVNNKEW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58627</accession>
    <name>Y1230_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1230</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1230</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64453">
        <property type="entry name" value="E64453"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58627"/>
    <dbReference type="STRING" id="243232.MJ_1230"/>
    <dbReference type="EnsemblBacteria" id="AAB99236">
        <property type="protein sequence ID" value="AAB99236"/>
        <property type="gene ID" value="MJ_1230"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1230"/>
    <dbReference type="eggNOG" id="arCOG05073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188846_1_0_2"/>
    <dbReference type="InParanoid" id="Q58627"/>
    <dbReference type="OMA" id="SCTNCGY"/>
    <dbReference type="PhylomeDB" id="Q58627"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018652">
        <property type="entry name" value="DUF2082_NA-bd_Znr"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09855">
        <property type="entry name" value="zinc_ribbon_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107227" description="Uncharacterized protein MJ1230">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <sequence length="76" mass="8903" checksum="2C53E9EABEF0247C" modified="1996-11-01" version="1">MVIFMEKRWKCPKCGNTEFFEKEVAMTGTGLSKIFDIQHNEYIVITCKKCGYSEFYDKSIVKSKDNLMNILDIFFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="140" xmlns="http://uniprot.org/uniprot">
    <accession>Q58659</accession>
    <name>SYM_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="1">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metG</name>
        <name type="ordered locus">MJ1263</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64457">
        <property type="entry name" value="F64457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870776.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58659"/>
    <dbReference type="SMR" id="Q58659"/>
    <dbReference type="STRING" id="243232.MJ_1263"/>
    <dbReference type="PRIDE" id="Q58659"/>
    <dbReference type="EnsemblBacteria" id="AAB99269">
        <property type="protein sequence ID" value="AAB99269"/>
        <property type="gene ID" value="MJ_1263"/>
    </dbReference>
    <dbReference type="GeneID" id="1452161"/>
    <dbReference type="KEGG" id="mja:MJ_1263"/>
    <dbReference type="eggNOG" id="arCOG00810">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_1_2_2"/>
    <dbReference type="InParanoid" id="Q58659"/>
    <dbReference type="OMA" id="SDMHGTP"/>
    <dbReference type="OrthoDB" id="6590at2157"/>
    <dbReference type="PhylomeDB" id="Q58659"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="PTHR45765"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="SSF57770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000139187" description="Methionine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA-binding" evidence="1">
        <location>
            <begin position="550"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="320"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <sequence length="651" mass="75559" checksum="C5C58DF5AA591E07" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58668</accession>
    <name>QUED_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative 6-carboxy-5,6,7,8-tetrahydropterin synthase</fullName>
            <shortName>CPH4 synthase</shortName>
            <ecNumber>4.1.2.50</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Archaeosine biosynthesis protein QueD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">queD</name>
        <name type="ordered locus">MJ1272</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin triphosphate (H2NTP) to 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) and acetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>7,8-dihydroneopterin 3'-triphosphate + H2O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + 2 H(+) + triphosphate</text>
            <dbReference type="Rhea" id="RHEA:27966"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18036"/>
            <dbReference type="ChEBI" id="CHEBI:58462"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="EC" id="4.1.2.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The active site is at the interface between 2 subunits. The proton acceptor Cys is on one subunit, and the charge relay system is on the other subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTPS family. QueD subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.50"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64458">
        <property type="entry name" value="G64458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870785.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58668"/>
    <dbReference type="SMR" id="Q58668"/>
    <dbReference type="STRING" id="243232.MJ_1272"/>
    <dbReference type="EnsemblBacteria" id="AAB99278">
        <property type="protein sequence ID" value="AAB99278"/>
        <property type="gene ID" value="MJ_1272"/>
    </dbReference>
    <dbReference type="GeneID" id="1452170"/>
    <dbReference type="KEGG" id="mja:MJ_1272"/>
    <dbReference type="eggNOG" id="arCOG02172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111016_3_0_2"/>
    <dbReference type="InParanoid" id="Q58668"/>
    <dbReference type="OMA" id="IGQGACY"/>
    <dbReference type="OrthoDB" id="104644at2157"/>
    <dbReference type="PhylomeDB" id="Q58668"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070497">
        <property type="term" value="F:6-carboxy-5,6,7,8-tetrahydropterin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PTHR12589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000057931" description="Putative 6-carboxy-5,6,7,8-tetrahydropterin synthase">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="163" mass="18816" checksum="3CDF37E98EF9C8CC" modified="1996-11-01" version="1">MMLELNGLHAGLRFSSAHIVFGHPTCGVIHGHSYYVDVKLYGERAGDFKFVCDFKIIKKIVKEICDELDHKLILPKNHEHVYYELRDKTLYFKYENKEYSIPVEDVILLPIPSTTAEDLAIYFANEIADRLKNLGFSNINWIEVSINEGIGQGACYRKYLEVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58693</accession>
    <name>BIOW_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">6-carboxyhexanoate--CoA ligase</fullName>
            <ecNumber evidence="1">6.2.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pimeloyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioW</name>
        <name type="ordered locus">MJ1297</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transformation of pimelate into pimeloyl-CoA with concomitant hydrolysis of ATP to AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CoA + heptanedioate = 6-carboxyhexanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14781"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:36165"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57360"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate metabolism; pimeloyl-CoA biosynthesis; pimeloyl-CoA from pimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BioW family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.14" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64461">
        <property type="entry name" value="H64461"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58693"/>
    <dbReference type="SMR" id="Q58693"/>
    <dbReference type="STRING" id="243232.MJ_1297"/>
    <dbReference type="DNASU" id="1452199"/>
    <dbReference type="EnsemblBacteria" id="AAB99304">
        <property type="protein sequence ID" value="AAB99304"/>
        <property type="gene ID" value="MJ_1297"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1297"/>
    <dbReference type="eggNOG" id="arCOG05075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076858_0_0_2"/>
    <dbReference type="InParanoid" id="Q58693"/>
    <dbReference type="OMA" id="LCWSDDP"/>
    <dbReference type="PhylomeDB" id="Q58693"/>
    <dbReference type="UniPathway" id="UPA00999">
        <property type="reaction ID" value="UER00351"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042410">
        <property type="term" value="F:6-carboxyhexanoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00668">
        <property type="entry name" value="BioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005499">
        <property type="entry name" value="BioW"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03744">
        <property type="entry name" value="BioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01204">
        <property type="entry name" value="bioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000191025" description="6-carboxyhexanoate--CoA ligase">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00668"/>
        </source>
    </evidence>
    <sequence length="237" mass="26884" checksum="A6B385A9524CA29D" modified="1996-11-01" version="1">MVIVMYSIKMRASKNGKHISGAERIVNKDEIEETVKELVRRALTHENGTPDFINIKIEEIKEEITYINHLPIKTIHCKDKEEARETAKKILRNEGIPDSVIDYAYEIIDKGGMRGAAILNLKGERLEPDKERGVRVKNIDTTKELKEKILKENLGTERTVDAIAIASKVIHLGVIAELCTSDNKSYTTGYVATKKGYFRITNLKKEGESGGRVFFVKDDVDIEDLINKLENKPFIIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58729</accession>
    <name>Y1333_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1333</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1333</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64466">
        <property type="entry name" value="D64466"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870850.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58729"/>
    <dbReference type="SMR" id="Q58729"/>
    <dbReference type="STRING" id="243232.MJ_1333"/>
    <dbReference type="EnsemblBacteria" id="AAB99345">
        <property type="protein sequence ID" value="AAB99345"/>
        <property type="gene ID" value="MJ_1333"/>
    </dbReference>
    <dbReference type="GeneID" id="1452235"/>
    <dbReference type="KEGG" id="mja:MJ_1333"/>
    <dbReference type="eggNOG" id="arCOG05077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519835_0_0_2"/>
    <dbReference type="OMA" id="MNRERKY"/>
    <dbReference type="OrthoDB" id="116298at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107278" description="Uncharacterized protein MJ1333">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="5"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="84" mass="9871" checksum="7C9F1E5079BBCF88" modified="1996-11-01" version="1">MPSPKIQEIINELDNLMNRERKYIELVATVEYLLNLIEPSKREKFKEALYDAETVEDVYELIKAIKLQLGMQGARRYLLTLEGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58744</accession>
    <name>Y1349_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1349</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1349</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FrhB family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64468">
        <property type="entry name" value="D64468"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58744"/>
    <dbReference type="SMR" id="Q58744"/>
    <dbReference type="STRING" id="243232.MJ_1349"/>
    <dbReference type="EnsemblBacteria" id="AAB99357">
        <property type="protein sequence ID" value="AAB99357"/>
        <property type="gene ID" value="MJ_1349"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1349"/>
    <dbReference type="eggNOG" id="arCOG02650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="InParanoid" id="Q58744"/>
    <dbReference type="OMA" id="AGSRYNW"/>
    <dbReference type="PhylomeDB" id="Q58744"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159228" description="Uncharacterized protein MJ1349">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="360" mass="40099" checksum="4F0046D70AA3482F" modified="1996-11-01" version="1">MRLMKSYKNLKEEVWDTNRCSGCGACVAVCPVNNLYFREESPVKFECDECSCIIVPADIVEHPISAEFCKTVVYDVPCGACYDACPRIKKSAIPKPKGLGNILKAVRAKASIEIKNAQNGGVVTAILANAFDEGLIDGAIVMMDDKWTLEPESYLALSKEDVLKSAGSKYLWKGPILKALKTAVMEKKLKKLAVVGTPCVINAIYQILSSDNDLLKPFREAIRLKIALFCFETYDYSKMIKKLNEDGIEPWEVKKMDIESGKLKITLINGNTVEYKLKDVESAMRNGCKVCGDFTGLTSDISVGNVGTEKGYSTVLIRNKWGEGFFKRAVYNGYITYDENVDLEAVEKLVELKKKRVKKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q58783</accession>
    <name>SIHH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">S-inosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="3">SIHH</shortName>
            <ecNumber evidence="1 2">3.13.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1388</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2015" name="J. Bacteriol." volume="197" first="2284" last="2291">
            <title>S-Inosyl-L-Homocysteine Hydrolase, a Novel Enzyme Involved in S-Adenosyl-L-Methionine Recycling.</title>
            <authorList>
                <person name="Miller D."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="25917907"/>
            <dbReference type="DOI" id="10.1128/JB.00080-15"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of S-inosyl-L-homocysteine (SIH) to L-homocysteine (Hcy) and inosine. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. Can also catalyze the reverse reaction in vitro, i.e. the synthesis of SIH from Hcy and inosine. Is specific for SIH and inosine as it is unable to either hydrolyze SAH or synthesize SAH from adenosine and Hcy.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>H2O + S-inosyl-L-homocysteine = inosine + L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:59828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1 4">
            <dbReference type="Rhea" id="RHEA:59829"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="2">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.64 mM for inosine (at pH 7.0 and 70 degrees Celsius)</KM>
            <KM evidence="2">0.005 mM for L-homocysteine (at pH 7.0 and 70 degrees Celsius)</KM>
            <KM evidence="2">0.22 mM for S-inosyl-L-homocysteine (at pH 7.0 and 70 degrees Celsius)</KM>
            <text evidence="2">kcat is 0.83 sec(-1) for the synthesis of SIH from inosine and L-homocysteine. kcat is 0.42 sec(-1) for the hydrolysis of SIH (at pH 7.0 and 70 degrees Celsius).</text>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 7.0-7.5 and pH 9.6 for the reaction in the direction of SIH synthesis. Shows no activity at either pH 6.5 or pH 11.5 in the synthetic direction. The activity of SIHH in the hydrolysis direction shows essentially the same activity over the pH range 6.5-11.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is about 70 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Exists both as a homotetramer and a homodimer, in a 4:1 ratio.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <dbReference type="EC" id="3.13.1.9" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64473">
        <property type="entry name" value="C64473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870905.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58783"/>
    <dbReference type="SMR" id="Q58783"/>
    <dbReference type="STRING" id="243232.MJ_1388"/>
    <dbReference type="EnsemblBacteria" id="AAB99397">
        <property type="protein sequence ID" value="AAB99397"/>
        <property type="gene ID" value="MJ_1388"/>
    </dbReference>
    <dbReference type="GeneID" id="1452291"/>
    <dbReference type="KEGG" id="mja:MJ_1388"/>
    <dbReference type="eggNOG" id="arCOG04137">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025194_2_1_2"/>
    <dbReference type="InParanoid" id="Q58783"/>
    <dbReference type="OMA" id="NKYGCRE"/>
    <dbReference type="OrthoDB" id="17629at2157"/>
    <dbReference type="PhylomeDB" id="Q58783"/>
    <dbReference type="BRENDA" id="3.13.1.9">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.3.1.B1">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="PTHR23420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000117004" description="S-inosyl-L-homocysteine hydrolase">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="212"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="291"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="25917907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="25917907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="25917907"/>
        </source>
    </evidence>
    <sequence length="415" mass="46607" checksum="16B1456E74D180F5" modified="1996-11-01" version="1">MYEVRDINLWKEGERKIQWAKQHMPVLNLIRERFKEEKPFKGITIGMALHLEAKTAVLAETLMEGGAEIAITGCNPLSTQDDVAAACAKKGMHVYAWRGETVEEYYENLNKVLDHKPDIVIDDGCDLIFLLHTKRTELLDNIMGGCEETTTGIIRLKAMEKEGALKFPVMDVNDAYTKHLFDNRYGTGQSALDGILRATNLLIAGKTVVVAGYGWCGRGVAMRAKGLGAEVVVTEVNPIRALEARMDGFRVMKMEKAAEIGDIFITTTGCKDVIRKEHILKMRNGAILANAGHFDNEINKKHLEELAKSIKEVRNCVTEYDLGNKKIYLLGEGRLVNLACADGHPCEVMDMSFANQALAAEYILKNHEKLEPRVYNIPYEQDLMIASLKLKAMGIEIDELTKEQKKYLEDWREGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58788</accession>
    <name>Y1393_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1393</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1393</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ1394 and A.fulgidus AF2028.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64473">
        <property type="entry name" value="H64473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870910.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58788"/>
    <dbReference type="STRING" id="243232.MJ_1393"/>
    <dbReference type="EnsemblBacteria" id="AAB99403">
        <property type="protein sequence ID" value="AAB99403"/>
        <property type="gene ID" value="MJ_1393"/>
    </dbReference>
    <dbReference type="GeneID" id="1452296"/>
    <dbReference type="KEGG" id="mja:MJ_1393"/>
    <dbReference type="eggNOG" id="arCOG06147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_445940_0_0_2"/>
    <dbReference type="InParanoid" id="Q58788"/>
    <dbReference type="OMA" id="VVWANCT"/>
    <dbReference type="OrthoDB" id="16428at2157"/>
    <dbReference type="PhylomeDB" id="Q58788"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107307" description="Uncharacterized protein MJ1393">
        <location>
            <begin position="1"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="608" mass="66768" checksum="010FAF1C29F8C73C" modified="1998-07-15" version="3"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58789</accession>
    <name>Y1394_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1394</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1394</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ1393 and A.fulgidus AF2028.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64474">
        <property type="entry name" value="A64474"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58789"/>
    <dbReference type="SMR" id="Q58789"/>
    <dbReference type="STRING" id="243232.MJ_1394"/>
    <dbReference type="EnsemblBacteria" id="AAB99404">
        <property type="protein sequence ID" value="AAB99404"/>
        <property type="gene ID" value="MJ_1394"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1394"/>
    <dbReference type="eggNOG" id="arCOG06147">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_303592_0_0_2"/>
    <dbReference type="InParanoid" id="Q58789"/>
    <dbReference type="OMA" id="DENFNGY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107308" description="Uncharacterized protein MJ1394">
        <location>
            <begin position="1"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="958"/>
            <end position="978"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="987" mass="112360" checksum="D1E628FFB28CA86D" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58856</accession>
    <name>Y1461_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1461</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1461</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1356.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64482">
        <property type="entry name" value="D64482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870981.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58856"/>
    <dbReference type="STRING" id="243232.MJ_1461"/>
    <dbReference type="PRIDE" id="Q58856"/>
    <dbReference type="EnsemblBacteria" id="AAB99475">
        <property type="protein sequence ID" value="AAB99475"/>
        <property type="gene ID" value="MJ_1461"/>
    </dbReference>
    <dbReference type="GeneID" id="1452365"/>
    <dbReference type="KEGG" id="mja:MJ_1461"/>
    <dbReference type="eggNOG" id="arCOG09681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369557_0_0_2"/>
    <dbReference type="OMA" id="YMEFEDR"/>
    <dbReference type="OrthoDB" id="100826at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107350" description="Uncharacterized protein MJ1461">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="199" mass="23821" checksum="B2E07FCE421FF069" modified="1996-11-01" version="1">MEQFDFDSIFNNAVGNMKYFIKKVKKYEEIKKHEDILKKDLLNAVNVFIERFRNNPCICKNRNNHSSCTTNACGEIENRMKNWVEKLFEYSDDEEKLNEFFKIIAKDAMKFVELDFEPLYILCGLEEIRETAEEKLKEELPTEEYLKVMEEFDDLIERMSLVATAVYMEFEDRVFERMGINKNLKYNIIKLGLKKMNIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58859</accession>
    <name>Y1464_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized GTP-binding protein MJ1464</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1464</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class YlqF/YawG GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64482">
        <property type="entry name" value="G64482"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58859"/>
    <dbReference type="SMR" id="Q58859"/>
    <dbReference type="STRING" id="243232.MJ_1464"/>
    <dbReference type="EnsemblBacteria" id="AAB99472">
        <property type="protein sequence ID" value="AAB99472"/>
        <property type="gene ID" value="MJ_1464"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1464"/>
    <dbReference type="eggNOG" id="arCOG00350">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011106_1_2_2"/>
    <dbReference type="InParanoid" id="Q58859"/>
    <dbReference type="OMA" id="EWQTGRF"/>
    <dbReference type="PhylomeDB" id="Q58859"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000205447" description="Uncharacterized GTP-binding protein MJ1464">
        <location>
            <begin position="1"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="CP-type G" evidence="2">
        <location>
            <begin position="14"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01058"/>
        </source>
    </evidence>
    <sequence length="373" mass="42635" checksum="469F4A1FF9111640" modified="1996-11-01" version="1">MMVRVMRYKKVPVKKIVNKIIDECDVILLVLDARDPEMTRNRELEKKIKAKGKKLIYVLNKADLVPKDILEKWKEVFGENTVFVSAKRRLGTKILREMIKQSLKEMGKKEGKVGIVGYPNVGKSSIINALTGKRKALTGSVAGLTKGEQWVRLTKNIKLMDTPGVLEMRDEDDLVISGALRLEKVENPIPPALKILSRINNFDNSIIKEYFGVDYEEVDEELLKKIGNKRSYLTKGGEVDLVRTAKTIIKEYQDGKLNYYKVDLKKYGQDRERDISFITKYLKDFPFIEDAKMIVTHLKDFDGLYKKIKKPVLGSEEIDGNIVVVSFGEKTKDACRKKVENLCRERNIEVLSKFGDKIGANNIYVAVGKRVKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58868</accession>
    <name>METE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine synthase</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Homocysteine methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Methylcobalamin:homocysteine methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">metE</name>
        <name type="ordered locus">MJ1473</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a methyl group to L-homocysteine resulting in methionine formation. Can use methylcobalamin and methylcobinamide as methyl donors, but methylcobalamin is not considered to be the physiological substrate.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the archaeal MetE family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99479" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99479.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64483">
        <property type="entry name" value="H64483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496838.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58868"/>
    <dbReference type="SMR" id="Q58868"/>
    <dbReference type="STRING" id="243232.MJ_1473"/>
    <dbReference type="EnsemblBacteria" id="AAB99479">
        <property type="protein sequence ID" value="AAB99479"/>
        <property type="gene ID" value="MJ_1473"/>
    </dbReference>
    <dbReference type="GeneID" id="1452378"/>
    <dbReference type="KEGG" id="mja:MJ_1473"/>
    <dbReference type="eggNOG" id="arCOG01876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040013_3_2_2"/>
    <dbReference type="InParanoid" id="Q58868"/>
    <dbReference type="OMA" id="DPDCGMR"/>
    <dbReference type="OrthoDB" id="21172at2157"/>
    <dbReference type="PhylomeDB" id="Q58868"/>
    <dbReference type="UniPathway" id="UPA00051"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00288">
        <property type="entry name" value="MetE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022921">
        <property type="entry name" value="MetE_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="SSF51726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098684" description="Methionine synthase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="308" mass="34724" checksum="6032FD5C97E2565D" modified="2001-01-11" version="2">MITTVVGSYPVVKKEETFLDKVKKVFGLYDEYKYAIERAVKDQVKAGVNIISDGQVRGDMVEIFTNNMYGFDGKRVVGRVEFIKPITLKDILYAKSIAKKLNPNVEIKGIITGPCTIASSVRVESCYSDNRDENLIYDIAKALRKEVEALKKHVPIIQIDEPILSTGMYDFDVARKAIDIIVDGLNIKFAMHVCGNVYNIIDELNKFNVDILDHEFASNKKNLVILESMEKKVGFGCVNTKVKKVESVEEIKSLIEEGIEILKNNEKLNKNLSDNILIDPDCGMRLLPIDVAFNKLKNMVEATKLIKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58870</accession>
    <name>Y1475_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1475</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1475</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64484">
        <property type="entry name" value="B64484"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870996.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58870"/>
    <dbReference type="SMR" id="Q58870"/>
    <dbReference type="STRING" id="243232.MJ_1475"/>
    <dbReference type="EnsemblBacteria" id="AAB99488">
        <property type="protein sequence ID" value="AAB99488"/>
        <property type="gene ID" value="MJ_1475"/>
    </dbReference>
    <dbReference type="GeneID" id="1452380"/>
    <dbReference type="KEGG" id="mja:MJ_1475"/>
    <dbReference type="eggNOG" id="arCOG03351">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976537_0_0_2"/>
    <dbReference type="InParanoid" id="Q58870"/>
    <dbReference type="OMA" id="EYHYYKL"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107358" description="Uncharacterized protein MJ1475">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <sequence length="125" mass="14634" checksum="AFB8CC9AFA1718DE" modified="1996-11-01" version="1">MKKLIAKTKEELIEKIKECENEEEIYINLELDRDVAIALLESCEPKKIYLPKSKYKRSSKKIIKALEGVDVEVIPIKAKTGRPTNVDKIIKKYLDKKPKEIAEITGINIKTVEYHYYKLKKKEKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58899</accession>
    <name>WECB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UDP-N-acetylglucosamine 2-epimerase</fullName>
            <ecNumber>5.1.3.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>UDP-GlcNAc-2-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">wecB</name>
        <name type="ordered locus">MJ1504</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible epimerization at C-2 of UDP-N-acetylglucosamine (UDP-GlcNAc) to produce UDP-N-acetylmannosamine (UDP-ManNAc), the activated donor of ManNAc residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine</text>
            <dbReference type="Rhea" id="RHEA:17213"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:68623"/>
            <dbReference type="EC" id="5.1.3.14"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UDP-N-acetylglucosamine 2-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.14"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64487">
        <property type="entry name" value="G64487"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871027.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4NEQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.85 A"/>
        <property type="chains" value="A=1-366"/>
    </dbReference>
    <dbReference type="PDB" id="4NES">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.42 A"/>
        <property type="chains" value="A=1-366"/>
    </dbReference>
    <dbReference type="PDBsum" id="4NEQ"/>
    <dbReference type="PDBsum" id="4NES"/>
    <dbReference type="AlphaFoldDB" id="Q58899"/>
    <dbReference type="SMR" id="Q58899"/>
    <dbReference type="STRING" id="243232.MJ_1504"/>
    <dbReference type="EnsemblBacteria" id="AAB99517">
        <property type="protein sequence ID" value="AAB99517"/>
        <property type="gene ID" value="MJ_1504"/>
    </dbReference>
    <dbReference type="GeneID" id="1452411"/>
    <dbReference type="KEGG" id="mja:MJ_1504"/>
    <dbReference type="eggNOG" id="arCOG01392">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041674_0_1_2"/>
    <dbReference type="InParanoid" id="Q58899"/>
    <dbReference type="OMA" id="RYNTERP"/>
    <dbReference type="OrthoDB" id="18594at2157"/>
    <dbReference type="PhylomeDB" id="Q58899"/>
    <dbReference type="BRENDA" id="5.1.3.14">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008761">
        <property type="term" value="F:UDP-N-acetylglucosamine 2-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="PTHR43174"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00236">
        <property type="entry name" value="wecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000208535" description="UDP-N-acetylglucosamine 2-epimerase">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="3"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="10"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="30"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="67"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="88"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="112"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="128"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="141"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="148"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="161"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="170"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="209"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="235"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="242"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="254"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="263"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="282"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="289"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="302"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="313"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="319"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="353"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4NES"/>
        </source>
    </evidence>
    <sequence length="366" mass="41726" checksum="E40DA8E87B835416" modified="1996-11-01" version="1">MKLSIILGTRPEIIKLSPIIRALEKTNIDWHIIHTNQHYSENMDKIFFEELNLPNPKYNLNIGSGTHGEQTGKMLIEIEKVLLKEKPDVVVVQGDTNTVLAGALVASKLKIDVAHVEAGLRSFDRNMPEEINRVLTDHISSYLFAPTEIAKNNLLREGIEENKIFVVGNTIVDATLQNLKIAEKNENVRAFFNSVVIDDDYFLLTLHRAENVDNKERLKNIVEGIFEIIEIYDKAIIFSIHPRTKKRLKEFNLFDKLKSNKKIKIIEPVGYLEFLMLEKNAELILTDSGGVQEEACILKVPCITLRDNTERPETVEVGANILVGDNKEKLIKAVEIMLNKKRNWKNPFGNGKSGERIVRILTYGKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58908</accession>
    <name>Y1513_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1513</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1513</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64488">
        <property type="entry name" value="H64488"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58908"/>
    <dbReference type="SMR" id="Q58908"/>
    <dbReference type="STRING" id="243232.MJ_1513"/>
    <dbReference type="PRIDE" id="Q58908"/>
    <dbReference type="EnsemblBacteria" id="AAB99535">
        <property type="protein sequence ID" value="AAB99535"/>
        <property type="gene ID" value="MJ_1513"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1513"/>
    <dbReference type="eggNOG" id="arCOG01449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_907966_0_0_2"/>
    <dbReference type="OMA" id="RRAEKVY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046260">
        <property type="entry name" value="DUF6293"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19810">
        <property type="entry name" value="DUF6293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107385" description="Uncharacterized protein MJ1513">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <sequence length="335" mass="38469" checksum="40BFB2D49842FF4C" modified="1996-11-01" version="1">MSKMTNDLEKIRGGVHIAVQGYEVDRITEVPIMRRAEKVYLICKPGNNDSKRGKAFKNVIIKKFEEKRVNYEIVEADLFDLDDIVKKMKLIIAHERKEFGDVKFYINVSSGSTIGCIAGITCAMILNKENSRIIPYYVMPEKSLDGLSEKEKEELKKEYESKYNCPYLPRSFGVRGVKLIYPFEVTLPREELLIFLKFIGRAGNRGLTIKELSILTKEEFLNVDLNDNESIKELIKAVERKESVSNSSVKKMKNLVRELKEVVGSDVDDLKKIITWRKKCRSSVSSTGQSDLVWVNKNVVEKLLELELIEKPEKIGKSKYIRISEKGKMLLNYVG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58929</accession>
    <name>MER_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5,10-methylenetetrahydromethanopterin reductase</fullName>
            <ecNumber evidence="1">1.5.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Methylene-H(4)MPT reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mer</name>
        <name type="ordered locus">MJ1534</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:21144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the mer family.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871058.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58929"/>
    <dbReference type="SMR" id="Q58929"/>
    <dbReference type="STRING" id="243232.MJ_1534"/>
    <dbReference type="PRIDE" id="Q58929"/>
    <dbReference type="EnsemblBacteria" id="AAB99555">
        <property type="protein sequence ID" value="AAB99555"/>
        <property type="gene ID" value="MJ_1534"/>
    </dbReference>
    <dbReference type="GeneID" id="1452442"/>
    <dbReference type="KEGG" id="mja:MJ_1534"/>
    <dbReference type="eggNOG" id="arCOG02410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027853_5_3_2"/>
    <dbReference type="InParanoid" id="Q58929"/>
    <dbReference type="OMA" id="VCWAAEA"/>
    <dbReference type="OrthoDB" id="46136at2157"/>
    <dbReference type="PhylomeDB" id="Q58929"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00697"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018537">
        <property type="term" value="F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01091">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019946">
        <property type="entry name" value="MeH4methanopterin_reductase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="SSF51679"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03555">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000084807" description="5,10-methylenetetrahydromethanopterin reductase">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01091"/>
        </source>
    </evidence>
    <sequence length="331" mass="35353" checksum="367B5DFD1EB6EBBD" modified="1998-06-01" version="2">MKFGIEFVPNEPIQKLCYYVKLAEDNGFEYCWITDHYNNRNVYMALTAIAMNTNKIKLGPGVTNPYVRSPAITASAIATLDELSGGRAVLGIGPGDKATFDALGIEWVKPVTTLKESIEVIRKLLAGERVSYEGKVVKIAGAALAVKPIQKAVPVYMGAQGPKMLETAGMIADGVLINASNPKDFEAAIPLIKKGAEAAGRSMDEIDVAAYACMSVDKNADKAKQAAVPVVAFIAAGSPPVVLERHGIDMEKVEAIRNALKSGNFPEAFKNVDDTMLEAFSIYGTPEDVVEKCKKLAEMGVTQIVAGSPIGPNKETAIKLIGKKVIPALKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58941</accession>
    <name>Y1546_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0219 protein MJ1546</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1546</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiolase-like superfamily. UPF0219 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64493">
        <property type="entry name" value="A64493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871070.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58941"/>
    <dbReference type="SMR" id="Q58941"/>
    <dbReference type="STRING" id="243232.MJ_1546"/>
    <dbReference type="EnsemblBacteria" id="AAB99564">
        <property type="protein sequence ID" value="AAB99564"/>
        <property type="gene ID" value="MJ_1546"/>
    </dbReference>
    <dbReference type="GeneID" id="1452454"/>
    <dbReference type="KEGG" id="mja:MJ_1546"/>
    <dbReference type="eggNOG" id="arCOG01767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039592_7_0_2"/>
    <dbReference type="InParanoid" id="Q58941"/>
    <dbReference type="OMA" id="YAKHKGK"/>
    <dbReference type="OrthoDB" id="12601at2157"/>
    <dbReference type="PhylomeDB" id="Q58941"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="UPF0219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="FabH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013528">
        <property type="entry name" value="HMG_CoA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01154">
        <property type="entry name" value="HMG_CoA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000057614" description="UPF0219 protein MJ1546">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <sequence length="345" mass="37549" checksum="8FDA1A9379064F0C" modified="2000-10-01" version="3">MAGIVGYGAYIPKYRIKVEEIARVWNKDPESIKKGLLVYEKAVPSLDEDTATIAVEAARNALKRAEIDPKDIGAVYVGSESHPYAVKPTATIVAEAIDATPDLTAADLEFACKAGTAGIQMCMGLVESGLIKYGLAIGADTAQGAPGDALEYTAAAGGAAYIIGKSNVIAEFNGTYSYTTDTPDFWRREGKPYPRHGGRFTGEPAYFRHVINAAKGLMEKMGTKPEDYDYCVFHQPNGKFYIRVAKILGFKEEQYKIGLLTPYIGNTYSGAVPLGLSNVLDNCEGGERILAVSYGSGAGSDAFDITVTDRINKVKDKAPKTAYYLERKEYIDYAIYAKFRKKIKM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58972</accession>
    <name>Y1577_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1577</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1577</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64496">
        <property type="entry name" value="H64496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871102.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58972"/>
    <dbReference type="STRING" id="243232.MJ_1577"/>
    <dbReference type="PRIDE" id="Q58972"/>
    <dbReference type="EnsemblBacteria" id="AAB99606">
        <property type="protein sequence ID" value="AAB99606"/>
        <property type="gene ID" value="MJ_1577"/>
    </dbReference>
    <dbReference type="GeneID" id="1452486"/>
    <dbReference type="KEGG" id="mja:MJ_1577"/>
    <dbReference type="eggNOG" id="arCOG03432">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_448801_0_0_2"/>
    <dbReference type="InParanoid" id="Q58972"/>
    <dbReference type="OMA" id="MWGEWLV"/>
    <dbReference type="OrthoDB" id="54190at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018702">
        <property type="entry name" value="DUF2207_membrane"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09972">
        <property type="entry name" value="DUF2207"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107423" description="Uncharacterized protein MJ1577">
        <location>
            <begin position="1"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="435"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="598" mass="68959" checksum="8B2AF84D60FEA45E" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58973</accession>
    <name>CBIF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt-precorrin-4 C(11)-methyltransferase</fullName>
            <ecNumber>2.1.1.271</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cobalt-precorrin-3 methylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cbiF</name>
        <name type="ordered locus">MJ1578</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Co-precorrin-4 + S-adenosyl-L-methionine = Co-precorrin-5A + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26277"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60061"/>
            <dbReference type="ChEBI" id="CHEBI:60062"/>
            <dbReference type="EC" id="2.1.1.271"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 4/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.271"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64497">
        <property type="entry name" value="A64497"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871103.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58973"/>
    <dbReference type="SMR" id="Q58973"/>
    <dbReference type="STRING" id="243232.MJ_1578"/>
    <dbReference type="EnsemblBacteria" id="AAB99599">
        <property type="protein sequence ID" value="AAB99599"/>
        <property type="gene ID" value="MJ_1578"/>
    </dbReference>
    <dbReference type="GeneID" id="1452487"/>
    <dbReference type="KEGG" id="mja:MJ_1578"/>
    <dbReference type="eggNOG" id="arCOG00645">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_1_2"/>
    <dbReference type="InParanoid" id="Q58973"/>
    <dbReference type="OMA" id="WSAMGEQ"/>
    <dbReference type="OrthoDB" id="67633at2157"/>
    <dbReference type="PhylomeDB" id="Q58973"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00226"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150399" description="Cobalt-precorrin-4 C(11)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="259" mass="29004" checksum="C5DB9E3C78143521" modified="1996-11-01" version="1">MDNNRKVIIVGAGPGDPELITIKGKKAIEEADVIIYAGSLVNEKLLEYNKKNAEIYNSANMNLEEIIDVMVKAVNQGKKVVRLHTGDPSIYGAIKEQIDELSKYGIDVEIIPGVSSLFAATASLKVELTLPEVSQTVIITRPEGRTPMPEKEKLRDLAKHQSTMAIFLGVSMIDKVVKELIEGGYREETPVAVVYHASWDDEKIVRGTLKDIAEKVKKEGIKKTALIIVGEVLNPKYYAYSKLYDKNFEHEYRKSHKKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58974</accession>
    <name>Y1579_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1579</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1579</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64497">
        <property type="entry name" value="B64497"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58974"/>
    <dbReference type="SMR" id="Q58974"/>
    <dbReference type="STRING" id="243232.MJ_1579"/>
    <dbReference type="EnsemblBacteria" id="AAB99607">
        <property type="protein sequence ID" value="AAB99607"/>
        <property type="gene ID" value="MJ_1579"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1579"/>
    <dbReference type="eggNOG" id="arCOG05091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_203607_0_0_2"/>
    <dbReference type="OMA" id="ETAPYVQ"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107424" description="Uncharacterized protein MJ1579">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <sequence length="70" mass="8483" checksum="A485930C4F232461" modified="1996-11-01" version="1">MNQRKEIELLMFDVLPYMANMEFIKELLESVNSLEELEQKVRELLEKETDITKKTDLKILLEKIEERKNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58978</accession>
    <name>Y1583_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable S-adenosyl-L-methionine-binding protein MJ1583</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1583</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the tRNA methyltransferase O family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64497">
        <property type="entry name" value="F64497"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871108.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58978"/>
    <dbReference type="SMR" id="Q58978"/>
    <dbReference type="STRING" id="243232.MJ_1583"/>
    <dbReference type="EnsemblBacteria" id="AAB99603">
        <property type="protein sequence ID" value="AAB99603"/>
        <property type="gene ID" value="MJ_1583"/>
    </dbReference>
    <dbReference type="GeneID" id="1452492"/>
    <dbReference type="KEGG" id="mja:MJ_1583"/>
    <dbReference type="eggNOG" id="arCOG00761">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013458_2_2_2"/>
    <dbReference type="InParanoid" id="Q58978"/>
    <dbReference type="OMA" id="QIQYFAR"/>
    <dbReference type="OrthoDB" id="117039at2157"/>
    <dbReference type="PhylomeDB" id="Q58978"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd09281">
        <property type="entry name" value="UPF0066"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023370">
        <property type="entry name" value="TrmO-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023368">
        <property type="entry name" value="UPF0066_cons_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040372">
        <property type="entry name" value="YaeB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036413">
        <property type="entry name" value="YaeB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036414">
        <property type="entry name" value="YaeB_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12818">
        <property type="entry name" value="PTHR12818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01980">
        <property type="entry name" value="TrmO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118196">
        <property type="entry name" value="SSF118196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00104">
        <property type="entry name" value="tRNA_TsaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01318">
        <property type="entry name" value="TSAA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51668">
        <property type="entry name" value="TSAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000155623" description="Probable S-adenosyl-L-methionine-binding protein MJ1583">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="TsaA-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="45"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6NDF6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="126" mass="14800" checksum="D707F0E57BBB2CF0" modified="1996-11-01" version="1">MYYLKPIGVVEQNENYTVLNIFDEFVEGLDGLKEGDYIIVLVWFHKNDSEEKRKILKVHPRGDINNPLKGVFATRSPYRPNPIGKYTVKIHKIYRNKIFIDKIDAYNETPIIDIKIFSEKLDCPKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q59013</accession>
    <name>Y1618_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1618</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1618</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64502">
        <property type="entry name" value="A64502"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59013"/>
    <dbReference type="SMR" id="Q59013"/>
    <dbReference type="STRING" id="243232.MJ_1618"/>
    <dbReference type="EnsemblBacteria" id="AAB99639">
        <property type="protein sequence ID" value="AAB99639"/>
        <property type="gene ID" value="MJ_1618"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1618"/>
    <dbReference type="eggNOG" id="arCOG03003">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129810_1_1_2"/>
    <dbReference type="InParanoid" id="Q59013"/>
    <dbReference type="OMA" id="PLKILCC"/>
    <dbReference type="PhylomeDB" id="Q59013"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107440" description="Uncharacterized protein MJ1618">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <sequence length="125" mass="14376" checksum="5B8402B3BDAC2404" modified="1996-11-01" version="1">MITMKIIKTEYDKIKPYITKDGSIIRELLHPNIYKGVKQSLAEAIVPVGSKTLLHKHYTSEEIYYILEGRGLMTLDNEKFEVKKGDTIYIPPKTPHKIENIGNVPLKILCCSYPPYSHEDTEILE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q59025</accession>
    <name>Y1631_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1631</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1631</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64503">
        <property type="entry name" value="E64503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871155.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59025"/>
    <dbReference type="SMR" id="Q59025"/>
    <dbReference type="STRING" id="243232.MJ_1631"/>
    <dbReference type="CAZy" id="GT35">
        <property type="family name" value="Glycosyltransferase Family 35"/>
    </dbReference>
    <dbReference type="DNASU" id="1452540"/>
    <dbReference type="EnsemblBacteria" id="AAB99652">
        <property type="protein sequence ID" value="AAB99652"/>
        <property type="gene ID" value="MJ_1631"/>
    </dbReference>
    <dbReference type="GeneID" id="1452540"/>
    <dbReference type="KEGG" id="mja:MJ_1631"/>
    <dbReference type="eggNOG" id="arCOG01421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015112_1_0_2"/>
    <dbReference type="InParanoid" id="Q59025"/>
    <dbReference type="OMA" id="LYRHGYF"/>
    <dbReference type="OrthoDB" id="19755at2157"/>
    <dbReference type="PhylomeDB" id="Q59025"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011834">
        <property type="entry name" value="Agluc_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02094">
        <property type="entry name" value="more_P_ylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000188563" description="Uncharacterized protein MJ1631">
        <location>
            <begin position="1"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="519" mass="60470" checksum="D2C9D64C7C0D2093" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q59032</accession>
    <name>Y1638_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1638</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1638</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64504">
        <property type="entry name" value="D64504"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59032"/>
    <dbReference type="SMR" id="Q59032"/>
    <dbReference type="STRING" id="243232.MJ_1638"/>
    <dbReference type="EnsemblBacteria" id="AAB99659">
        <property type="protein sequence ID" value="AAB99659"/>
        <property type="gene ID" value="MJ_1638"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1638"/>
    <dbReference type="eggNOG" id="arCOG00045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077587_0_0_2"/>
    <dbReference type="InParanoid" id="Q59032"/>
    <dbReference type="OMA" id="YHPCGRC"/>
    <dbReference type="PhylomeDB" id="Q59032"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012096">
        <property type="entry name" value="ATPase_PP-loop_MJ1638"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006601">
        <property type="entry name" value="ATPase_UCP006601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107452" description="Uncharacterized protein MJ1638">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <sequence length="260" mass="30016" checksum="8F74C0A746676051" modified="1996-11-01" version="1">MNFEKIIEEKVNQKLKELRLKNSLEILEKLDINTELKEALKSTLLKRLNGEKEFYKISIDKKPKAVVAFSGGVDSSTSAIIAKQIFDVKAVSCYSKYIMTDEMRENAKNIAKKIGISLEFVSIDLEEVYKGVVNGKFHPCGRCHKVIENAVIDYAKKIDAEFVIFGDLLAFGYLALYREDEIFRFNLPSFFALTKDEEREILKNNGIELKMSYGCPLLKIYHKHNKGYKFTIQRILREVRGRVVNEEEGFKNIVEILNQQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q59033</accession>
    <name>Y1639_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1639</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1639</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64504">
        <property type="entry name" value="E64504"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59033"/>
    <dbReference type="EnsemblBacteria" id="AAB99665">
        <property type="protein sequence ID" value="AAB99665"/>
        <property type="gene ID" value="MJ_1639"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1639"/>
    <dbReference type="HOGENOM" id="CLU_1700289_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107453" description="Uncharacterized protein MJ1639">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <sequence length="154" mass="17584" checksum="594DBEA256F4EDA7" modified="1996-11-01" version="1">MYFVIKGDSIMYFVLRLGGDLSLYIPAKYRPFFEPLDKNSPYNIKVNLENSSGDIVVDNVLDDKKIDERNSEITKLGGLINELRRKKENGDNINIIEIRLKLKDDSIITHNLNTPNIEDYDDNKLVINSGTLVINGKTTIDLNNIEEITLKLDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q59073</accession>
    <name>Y1679_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1679</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1679</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64509">
        <property type="entry name" value="E64509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871203.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59073"/>
    <dbReference type="SMR" id="Q59073"/>
    <dbReference type="STRING" id="243232.MJ_1679"/>
    <dbReference type="PRIDE" id="Q59073"/>
    <dbReference type="EnsemblBacteria" id="AAB99704">
        <property type="protein sequence ID" value="AAB99704"/>
        <property type="gene ID" value="MJ_1679"/>
    </dbReference>
    <dbReference type="GeneID" id="1452588"/>
    <dbReference type="KEGG" id="mja:MJ_1679"/>
    <dbReference type="eggNOG" id="arCOG01011">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2461944_0_0_2"/>
    <dbReference type="InParanoid" id="Q59073"/>
    <dbReference type="OMA" id="GQIMMEK"/>
    <dbReference type="OrthoDB" id="106015at2157"/>
    <dbReference type="PhylomeDB" id="Q59073"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041088">
        <property type="entry name" value="RHH_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016748">
        <property type="entry name" value="Tscrpt_reg_CopG_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17723">
        <property type="entry name" value="RHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019108">
        <property type="entry name" value="Txn_reg_CopG_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107479" description="Uncharacterized protein MJ1679">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <sequence length="97" mass="11880" checksum="64C59303B9101FE0" modified="1996-11-01" version="1">MIEEEITLKIPKSIVNQIDELIKEGYFSSRDEFVRYAVRESLTEIAISKKMSREECKKIWEEYKIRKKDIKIDEKEIEELLDEVDKEWKKWKKLKLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q60259</accession>
    <name>Y3522_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL22</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64512">
        <property type="entry name" value="E64512"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890069.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60259"/>
    <dbReference type="SMR" id="Q60259"/>
    <dbReference type="STRING" id="243232.MJ_ECL22"/>
    <dbReference type="EnsemblBacteria" id="AAC37093">
        <property type="protein sequence ID" value="AAC37093"/>
        <property type="gene ID" value="MJ_ECL22"/>
    </dbReference>
    <dbReference type="GeneID" id="1450806"/>
    <dbReference type="KEGG" id="mja:MJ_ECL22"/>
    <dbReference type="HOGENOM" id="CLU_2597744_0_0_2"/>
    <dbReference type="OrthoDB" id="26059at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01011">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107510" description="Uncharacterized protein MJECL22">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <sequence length="79" mass="9201" checksum="3C7D0F6F65B7AE2D" modified="1997-11-01" version="1">MLELRSLSIRGGIAVLECGRRCISAINLKTGDKIWEFKTEWDIESISIKDNRVMLKCNGRRHIYIDLKTGRKIRELIIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>Q60261</accession>
    <name>Y3506_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL06</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL06</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64510">
        <property type="entry name" value="F64510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890053.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60261"/>
    <dbReference type="SMR" id="Q60261"/>
    <dbReference type="EnsemblBacteria" id="AAC37079">
        <property type="protein sequence ID" value="AAC37079"/>
        <property type="gene ID" value="MJ_ECL06"/>
    </dbReference>
    <dbReference type="GeneID" id="1450792"/>
    <dbReference type="KEGG" id="mja:MJ_ECL06"/>
    <dbReference type="eggNOG" id="arCOG10970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2091314_0_0_2"/>
    <dbReference type="OMA" id="MCNIALA"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107498" description="Uncharacterized protein MJECL06">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <sequence length="116" mass="13827" checksum="A71627F7FFA2ED0F" modified="1997-11-01" version="1">MSVELRTLFRLIAVLEHSEEFKKVLFACERHFESGYCKCGPMEMCNIALAEAMKEDPTLVLRKWRRVFTYLEEVGIIKTRKLEAPANRPRRYIKLSENWMEALRTAIDKEYEKLIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q60285</accession>
    <name>Y3526_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJECL26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL26</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64513">
        <property type="entry name" value="A64513"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60285"/>
    <dbReference type="DNASU" id="1450810"/>
    <dbReference type="EnsemblBacteria" id="AAC37097">
        <property type="protein sequence ID" value="AAC37097"/>
        <property type="gene ID" value="MJ_ECL26"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_ECL26"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q60285"/>
    <dbReference type="OMA" id="EWHAIRE"/>
    <dbReference type="PhylomeDB" id="Q60285"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184680" description="Uncharacterized ATP-binding protein MJECL26">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="343" mass="40442" checksum="EC7181030B46D227" modified="1997-11-01" version="1">MGEIMKFYDREKELNYLKTYCQLEPNSILFVYGPKSSGKSTVMRRVIKELENSNIVFFYYNLRKYATPTRDEFLRVFFEKSDKKYLLNKLELNLGVCKFGIEENFDFNNLCLNDVFAKINESINAVVEEGKKPVLIIDELQKLKNIYFNGGKSLLNELFNLFVSLTKMEHLCHVICLTSDTLFIDEIYRNSTLKNASEYYLIDWLRKGTIRNILKEEGFSEEEINYALDYLSLPYEISQLINNKKLGLSVEQTIKQWINVEKDGLKYLIDTTDLDEEGLYNVLSKFKDKIKISYDKEVKKEEMKYLKFLIENEILFYDVINGIIKPTSIIEWHAIKEIINAMQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q60323</accession>
    <name>Y017_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0017</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0017</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64302">
        <property type="entry name" value="A64302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869509.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60323"/>
    <dbReference type="STRING" id="243232.MJ_0017"/>
    <dbReference type="EnsemblBacteria" id="AAB97994">
        <property type="protein sequence ID" value="AAB97994"/>
        <property type="gene ID" value="MJ_0017"/>
    </dbReference>
    <dbReference type="GeneID" id="1450855"/>
    <dbReference type="KEGG" id="mja:MJ_0017"/>
    <dbReference type="eggNOG" id="arCOG02134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079548_0_0_2"/>
    <dbReference type="InParanoid" id="Q60323"/>
    <dbReference type="OMA" id="DGYGHQT"/>
    <dbReference type="OrthoDB" id="101978at2157"/>
    <dbReference type="PhylomeDB" id="Q60323"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032874">
        <property type="entry name" value="DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13610">
        <property type="entry name" value="DDE_Tnp_IS240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106657" description="Uncharacterized protein MJ0017">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <sequence length="214" mass="25506" checksum="0F135E2C748F933D" modified="1997-11-01" version="1">MRLTIEVIKEKIVERKLFKRNRKSIEVKILAGLLYYLGLSLRKVSLFLSQFEDISHESVRIYYHKIKEVLNEPERKERNLIAIDEIKLKVGDKYIYAWSAIDVETKECLGVYISKTRNYLDTILFVKSILKFCSNKPKILVDGGKWYPWALRKLGLEFEKVKFGLRNCVESFFSVLKRRTKAFFNRFPNNSKFDTVISWIKSFMMFYNWVKSLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q60330</accession>
    <name>Y015_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0015</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0015</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64301">
        <property type="entry name" value="G64301"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60330"/>
    <dbReference type="SMR" id="Q60330"/>
    <dbReference type="STRING" id="243232.MJ_0015"/>
    <dbReference type="EnsemblBacteria" id="AAB97993">
        <property type="protein sequence ID" value="AAB97993"/>
        <property type="gene ID" value="MJ_0015"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0015"/>
    <dbReference type="eggNOG" id="arCOG07384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2327284_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q60330"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004446">
        <property type="entry name" value="Heptose_bisP_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891">
        <property type="entry name" value="PTHR42891"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106655" description="Uncharacterized protein MJ0015">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <sequence length="98" mass="11733" checksum="428760FF9D57D475" modified="1997-11-01" version="1">MMRLNWQIRQVQLLLERLGLQQLVWMDCLVMMLNKAIFLDRDGVINKRLIGDYVKKIEEFELLPNVREALIEFKKMGYLLIVVTNQQGSKQFLIFYNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>P54012</accession>
    <name>RS15_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps15</name>
        <name type="ordered locus">MJ0036</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869527.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54012"/>
    <dbReference type="SMR" id="P54012"/>
    <dbReference type="STRING" id="243232.MJ_0036"/>
    <dbReference type="EnsemblBacteria" id="AAB98017">
        <property type="protein sequence ID" value="AAB98017"/>
        <property type="gene ID" value="MJ_0036"/>
    </dbReference>
    <dbReference type="GeneID" id="1450874"/>
    <dbReference type="KEGG" id="mja:MJ_0036"/>
    <dbReference type="eggNOG" id="arCOG04185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090139_2_0_2"/>
    <dbReference type="InParanoid" id="P54012"/>
    <dbReference type="OMA" id="EEHPKDM"/>
    <dbReference type="OrthoDB" id="98179at2157"/>
    <dbReference type="PhylomeDB" id="P54012"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_S15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_S13/S15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_S15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_S15P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="S15_NS1_RNA-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="PTHR11885"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="SSF47060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000115606" description="30S ribosomal protein S15">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="153" mass="17845" checksum="7C1DAEA164CDEA39" modified="1998-07-15" version="2">MARMHARKRGRSGSKRPVRKEVPEWVQYTPEQVEQLVVELAKKGYQSAQIGLILRDTYGIPDVKLITGKKISKIMKEHGLYPKVPEDLLNLMRRAVNLRKHLEQHPKDLHSKRGLQLIESKIRRLVKYYKSKGVLPADWRYTPETARLLVEQA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>P54033</accession>
    <name>RL22_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl22</name>
        <name type="ordered locus">MJ0460</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64357">
        <property type="entry name" value="D64357"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54033"/>
    <dbReference type="SMR" id="P54033"/>
    <dbReference type="STRING" id="243232.MJ_0460"/>
    <dbReference type="EnsemblBacteria" id="AAB98449">
        <property type="protein sequence ID" value="AAB98449"/>
        <property type="gene ID" value="MJ_0460"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0460"/>
    <dbReference type="eggNOG" id="arCOG04098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083987_0_2_2"/>
    <dbReference type="InParanoid" id="P54033"/>
    <dbReference type="OMA" id="ANAEYKG"/>
    <dbReference type="PhylomeDB" id="P54033"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_L22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_L22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_L22/L17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_L22/L17_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_L22/L17_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="PTHR11593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="SSF54843"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125275" description="50S ribosomal protein L22">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <sequence length="156" mass="18039" checksum="58745761B5472C4E" modified="1996-10-01" version="1">MIMMGKLKYKIQVNPEKTARAMGRNIPISRKHAREICKSINGMKLDEAIKFLEDVIAMRRPVLFRRHCKKVGHRKGKLGWPAGRYPVKAAKAILKILQHAKANAEYKGLNTEKLRIKHISTNKGITIKRYMPRAFGRATPKFQETVHIQVILEEYH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>P54056</accession>
    <name>RL39_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L39e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl39e</name>
        <name type="ordered locus">MJ0689</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98684" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98684.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64386">
        <property type="entry name" value="A64386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496580.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54056"/>
    <dbReference type="STRING" id="243232.MJ_0689"/>
    <dbReference type="EnsemblBacteria" id="AAB98684">
        <property type="protein sequence ID" value="AAB98684"/>
        <property type="gene ID" value="MJ_0689"/>
    </dbReference>
    <dbReference type="GeneID" id="1451555"/>
    <dbReference type="KEGG" id="mja:MJ_0689"/>
    <dbReference type="eggNOG" id="arCOG04177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181948_4_0_2"/>
    <dbReference type="InParanoid" id="P54056"/>
    <dbReference type="OMA" id="KMRYWRR"/>
    <dbReference type="OrthoDB" id="131584at2157"/>
    <dbReference type="PhylomeDB" id="P54056"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_L39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020083">
        <property type="entry name" value="Ribosomal_L39_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_L39e_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="SSF48662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00051">
        <property type="entry name" value="RIBOSOMAL_L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000127050" description="50S ribosomal protein L39e">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="51" mass="6144" checksum="CEF905E1570B3B30" modified="2003-03-28" version="2">MGSNKPLGKKVRLAKALKQNRRVPLFVIVKTRGRVRFHPKMRYWRRKKLKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P54064</accession>
    <name>RL24E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl24e</name>
        <name type="ordered locus">MJ1201</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64449">
        <property type="entry name" value="H64449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870713.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54064"/>
    <dbReference type="SMR" id="P54064"/>
    <dbReference type="STRING" id="243232.MJ_1201"/>
    <dbReference type="EnsemblBacteria" id="AAB99205">
        <property type="protein sequence ID" value="AAB99205"/>
        <property type="gene ID" value="MJ_1201"/>
    </dbReference>
    <dbReference type="GeneID" id="8804674"/>
    <dbReference type="KEGG" id="mja:MJ_1201"/>
    <dbReference type="eggNOG" id="arCOG01950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190191_0_0_2"/>
    <dbReference type="InParanoid" id="P54064"/>
    <dbReference type="OMA" id="GTGKMYV"/>
    <dbReference type="OrthoDB" id="125162at2157"/>
    <dbReference type="PhylomeDB" id="P54064"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023438">
        <property type="entry name" value="Ribosomal_L24e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_L24e-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_L24e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="PTHR10792"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000136916" description="50S ribosomal protein L24e">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <sequence length="70" mass="8249" checksum="3EB6DE18F26E6FCF" modified="1996-10-01" version="1">MPEWRTCSFCGYEIEPGKGKMVVEKDGTVLYFCSSKCEKSYRMGRNPRKLKWTKVYQDMKAELKKAQESQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>P58416</accession>
    <accession>Q57704</accession>
    <name>COME_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfopyruvate decarboxylase subunit beta</fullName>
            <ecNumber evidence="1">4.1.1.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">comE</name>
        <name type="ordered locus">MJ0256</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="4862" last="4867">
            <title>Identification of the gene encoding sulfopyruvate decarboxylase, an enzyme involved in biosynthesis of coenzyme M.</title>
            <authorList>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10940029"/>
            <dbReference type="DOI" id="10.1128/JB.182.17.4862-4867.2000"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>COFACTOR</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the coenzyme M (2-mercaptoethanesulfonic acid). Catalyzes the decarboxylation of sulfopyruvate to sulfoacetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-sulfopyruvate + H(+) = CO2 + sulfoacetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:20948"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57940"/>
            <dbReference type="ChEBI" id="CHEBI:58246"/>
            <dbReference type="EC" id="4.1.1.79"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="1">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by oxygen when heated in air at 80 degrees Celsius. The enzyme is reactivated by addition of dithionite.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">0.64 mM for 3-sulfopyruvate</KM>
            <Vmax evidence="1">52.0 umol/min/mg enzyme</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterododecamer composed of 6 subunits alpha and 6 subunits beta.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence corresponding to this entry was originally entered in Swiss-Prot as AC Q57704 in November 1997 and was deleted in July 1999 because TIGR removed the CDS for that ORF. We have recreated it because of the evidence (PubMed:10940029) that it really exists.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.79" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64332">
        <property type="entry name" value="A64332"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496438.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58416"/>
    <dbReference type="SMR" id="P58416"/>
    <dbReference type="GeneID" id="27929949"/>
    <dbReference type="InParanoid" id="P58416"/>
    <dbReference type="OMA" id="GSVLMNM"/>
    <dbReference type="OrthoDB" id="84988at2157"/>
    <dbReference type="PhylomeDB" id="P58416"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-2266"/>
    <dbReference type="SABIO-RK" id="P58416"/>
    <dbReference type="UniPathway" id="UPA00355">
        <property type="reaction ID" value="UER00472"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd03372">
        <property type="entry name" value="TPP_ComE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022494">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03846">
        <property type="entry name" value="sulfopy_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="chain" id="PRO_0000090839" description="Sulfopyruvate decarboxylase subunit beta">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="10940029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="10940029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="10940029"/>
        </source>
    </evidence>
    <sequence length="188" mass="20980" checksum="52A6C91E2D43B97D" modified="2001-12-05" version="1">MYPKRIDIIKKIVENVGEKEIIVSNIGIPSKELYYVKDRERNFYMLGSMGLASSIGLGLALNCEDKVIVIDGDGSILMNLGSLSTIGYMNPKNYILVIIDNSAYGSTGNQKTHTGKNTNLEEIAKGCGLDTITTESLEEFEKEFKNALNEEKCKVIIAKTIPYNEKCSNIEIPPVVLKYRFMEAIKRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>P81248</accession>
    <name>YF7B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1572.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1572.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871097.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81248"/>
    <dbReference type="SMR" id="P81248"/>
    <dbReference type="STRING" id="243232.MJ_1572.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99596">
        <property type="protein sequence ID" value="AAB99596"/>
        <property type="gene ID" value="MJ_1572.1"/>
    </dbReference>
    <dbReference type="GeneID" id="63606508"/>
    <dbReference type="KEGG" id="mja:MJ_1572.1"/>
    <dbReference type="eggNOG" id="arCOG00555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140207_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018973">
        <property type="entry name" value="MZB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09369">
        <property type="entry name" value="DUF1998"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107420" description="Uncharacterized protein MJ1572.1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <sequence length="112" mass="12917" checksum="9F6AA26CD7BCE636" modified="1998-06-01" version="1">MHAIEHNIIKITPIFTYIDSREIGGYSYERFNRNLFKDKAVIFIYDGNEGGFGLAEILYENAEKLLNKSLEHLKNCNCADGCPLCIYSTKCGTFNEFLDKWQAIRILEKLLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>P81291</accession>
    <name>LEUC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Isopropylmalate/citramalate isomerase large subunit</fullName>
            <ecNumber evidence="1">4.2.1.33</ecNumber>
            <ecNumber>4.2.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>(R)-2-methylmalate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>(R)-citramalate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>3-isopropylmalate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Alpha-isopropylmalate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Citraconate hydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopropylmalate isomerase</fullName>
            <shortName evidence="1">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Maleate hydratase</fullName>
            <shortName>Malease</shortName>
            <ecNumber>4.2.1.31</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuC</name>
        <name type="ordered locus">MJ0499</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="J. Bacteriol." volume="189" first="4391" last="4400">
            <title>Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Drevland R.M."/>
                <person name="Waheed A."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="17449626"/>
            <dbReference type="DOI" id="10.1128/JB.00166-07"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate, a step involved in leucine biosynthesis. Catalyzes the isomerization between 2-methylmalate and 3-methylmalate, via the formation of 2-methylmaleate (citraconate), a step involved in isoleucine biosynthesis. Also displays malease activity, i.e. catalyzes the hydration of maleate to form (R)-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(3R)-citramalate = 2-methylmaleate + H2O</text>
            <dbReference type="Rhea" id="RHEA:22332"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30719"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="EC" id="4.2.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-methylmaleate + H2O = (2R,3S)-3-methylmalate</text>
            <dbReference type="Rhea" id="RHEA:42424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30719"/>
            <dbReference type="ChEBI" id="CHEBI:58511"/>
            <dbReference type="EC" id="4.2.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-malate = H2O + maleate</text>
            <dbReference type="Rhea" id="RHEA:23692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:30780"/>
            <dbReference type="EC" id="4.2.1.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1 2">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">80 uM for 2-methylmaleate</KM>
            <KM evidence="2">810 uM for (R)-2-methylmalate</KM>
            <KM evidence="2">1900 uM for racemic 2-isopropylmalate</KM>
            <KM evidence="2">39 uM for racemic 3-isopropylmalate</KM>
            <KM evidence="2">400 uM for maleate</KM>
            <Vmax evidence="2">15.0 umol/min/mg enzyme for 2-methylmaleate hydration reaction</Vmax>
            <Vmax evidence="2">1.5 umol/min/mg enzyme for (R)-2-methylmalate dehydration reaction</Vmax>
            <Vmax evidence="2">4.2 umol/min/mg enzyme for 2-isopropylmalate dehydration reaction</Vmax>
            <Vmax evidence="2">1.8 umol/min/mg enzyme for 3-isopropylmalate dehydration reaction</Vmax>
            <Vmax evidence="2">34.0 umol/min/mg enzyme for maleate hydration reaction</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 7.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 70 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of 2 LeuC and 2 LeuD. Cannot form a complex with HacB.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="1"/>
    <dbReference type="EC" id="4.2.1.35"/>
    <dbReference type="EC" id="4.2.1.31"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64362">
        <property type="entry name" value="C64362"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870000.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4KP1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=1-424"/>
    </dbReference>
    <dbReference type="PDB" id="4NQY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B=1-424"/>
    </dbReference>
    <dbReference type="PDBsum" id="4KP1"/>
    <dbReference type="PDBsum" id="4NQY"/>
    <dbReference type="AlphaFoldDB" id="P81291"/>
    <dbReference type="SMR" id="P81291"/>
    <dbReference type="STRING" id="243232.MJ_0499"/>
    <dbReference type="DNASU" id="1451361"/>
    <dbReference type="EnsemblBacteria" id="AAB98487">
        <property type="protein sequence ID" value="AAB98487"/>
        <property type="gene ID" value="MJ_0499"/>
    </dbReference>
    <dbReference type="GeneID" id="1451361"/>
    <dbReference type="KEGG" id="mja:MJ_0499"/>
    <dbReference type="eggNOG" id="arCOG01698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="InParanoid" id="P81291"/>
    <dbReference type="OMA" id="CNMSIEM"/>
    <dbReference type="OrthoDB" id="15714at2157"/>
    <dbReference type="PhylomeDB" id="P81291"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-13647"/>
    <dbReference type="BRENDA" id="4.2.1.33">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="4.2.1.36">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="P81291"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00067"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047508">
        <property type="term" value="F:(R)-2-methylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050075">
        <property type="term" value="F:maleate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000076869" description="Isopropylmalate/citramalate isomerase large subunit">
        <location>
            <begin position="1"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="5"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="24"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="30"/>
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        </location>
    </feature>
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        <location>
            <begin position="38"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="59"/>
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        </location>
    </feature>
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        <location>
            <begin position="73"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="93"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="102"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="117"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
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        </location>
    </feature>
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    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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    <feature type="helix" evidence="3">
        <location>
            <begin position="365"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="381"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="393"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="396"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="400"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="406"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="421"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17449626"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4KP1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4NQY"/>
        </source>
    </evidence>
    <sequence length="424" mass="46107" checksum="B4D106AE165C524C" modified="1998-07-15" version="1">MGMTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNPEKIVILFDHQVPADSIKAAENHILMRKFVKEQGIKYFYDIREGVCHQVLPEKGHVAPGEVVVGADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETIYFNITGDLQPYVTSKDVILSIIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKEAMKKHGTERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAREVAGKPIDQVFIGSCTNGRLEDLRMAIKIIEKHGGIADDVRVVVTPASREEYLKALKEGIIEKFLKYGCVVTNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAEIYLASPITAAACAVKGELVDPRDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>P81311</accession>
    <name>Y70A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0703.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0703.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81311"/>
    <dbReference type="SMR" id="P81311"/>
    <dbReference type="STRING" id="243232.MJ_0703.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98706">
        <property type="protein sequence ID" value="AAB98706"/>
        <property type="gene ID" value="MJ_0703.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0703.1"/>
    <dbReference type="HOGENOM" id="CLU_2271063_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106996" description="Uncharacterized protein MJ0703.1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="102" mass="11685" checksum="1A1A282B05A7CBA0" modified="1998-07-15" version="1">MVGNMNIRDKIKSIKNWINFIKPIITIVGIVISAVAFTISILWGMLFLILFLILITFSKTIRKILSKKERSYQGLILSIIGSIIIISIIVYSHCYIEFKLLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57563</accession>
    <name>ASSTI_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L-aspartate semialdehyde sulfurtransferase iron-sulfur subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0099</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for O-acetylhomoserine sulfhydrylase (OAHS)-independent homocysteine (Hcy) biosynthesis. Together with MJ0100, catalyzes the condensation of sulfide with aspartate semialdehyde to generate homocysteine. May be involved in the reduction of the disulfide formed in MJ0100.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 2 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">May form a complex with MJ0100.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64312">
        <property type="entry name" value="C64312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869591.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57563"/>
    <dbReference type="SMR" id="Q57563"/>
    <dbReference type="STRING" id="243232.MJ_0099"/>
    <dbReference type="EnsemblBacteria" id="AAB98079">
        <property type="protein sequence ID" value="AAB98079"/>
        <property type="gene ID" value="MJ_0099"/>
    </dbReference>
    <dbReference type="GeneID" id="1450938"/>
    <dbReference type="KEGG" id="mja:MJ_0099"/>
    <dbReference type="eggNOG" id="arCOG02460">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152999_0_0_2"/>
    <dbReference type="InParanoid" id="Q57563"/>
    <dbReference type="OMA" id="TGVCPTE"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="PhylomeDB" id="Q57563"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018449">
        <property type="entry name" value="NIL_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09383">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00930">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159130" description="L-aspartate semialdehyde sulfurtransferase iron-sulfur subunit">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8TPT3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="131" mass="15105" checksum="BDE45A4CD055B78E" modified="1997-11-01" version="1">MRKRVYYWTDSEHINKPVISDTILNTGVKINILKAKVEPQEAFLILELFGSKETIEKALNYLSKFGEVEEISKVIKRDLEKCVHCGCCITQCPINVIYMDEDYNVVFKEEDCVGCKNCLKACPFKAIEIFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q57584</accession>
    <name>Y120_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0120</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0120</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64314">
        <property type="entry name" value="H64314"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869613.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57584"/>
    <dbReference type="SMR" id="Q57584"/>
    <dbReference type="STRING" id="243232.MJ_0120"/>
    <dbReference type="EnsemblBacteria" id="AAB98101">
        <property type="protein sequence ID" value="AAB98101"/>
        <property type="gene ID" value="MJ_0120"/>
    </dbReference>
    <dbReference type="GeneID" id="1450963"/>
    <dbReference type="KEGG" id="mja:MJ_0120"/>
    <dbReference type="eggNOG" id="arCOG01231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1193419_0_0_2"/>
    <dbReference type="InParanoid" id="Q57584"/>
    <dbReference type="OMA" id="DMCPDHF"/>
    <dbReference type="OrthoDB" id="56639at2157"/>
    <dbReference type="PhylomeDB" id="Q57584"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="PTHR30134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106702" description="Uncharacterized protein MJ0120">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <sequence length="233" mass="25933" checksum="0FE4552D596E0937" modified="1997-11-01" version="1">MKVAIVAGTPGAGKTSVLIHTIRTLINEGYKPVVVKIDCLYTDDDVRYKKLGIPVLVGLSKDMCPDHFAIYNFEEMVDWAKDKGDILLIETAGLCHRCAPYTKNSLGICVIDATSGPNTPRKVGPFLTSADIVVITKGDIISQAEREVFRERVLEMNPNCRIYEVNGLTGQGCVEIAKEIIESKDIKDLENEELRHNAPLCICTLCVGETRVSKKYHRGILRRIDGFMEYEGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57609</accession>
    <name>GCH3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>GTP cyclohydrolase III</fullName>
            <ecNumber>3.5.4.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>MjGC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">gch3</name>
        <name type="ordered locus">MJ0145</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Biochemistry" volume="41" first="15074" last="15084">
            <title>A member of a new class of GTP cyclohydrolases produces formylaminopyrimidine nucleotide monophosphates.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="12475257"/>
            <dbReference type="DOI" id="10.1021/bi0268798"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>MUTAGENESIS OF HIS-136</scope>
        <scope>MASS SPECTROMETRY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Catalyzes the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone ribonucleotide monophosphate and inorganic phosphate from GTP. Also has an independent pyrophosphate phosphohydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:22468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="EC" id="3.5.4.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text>Homotrimer.</text>
    </comment>
    <comment type="mass spectrometry" mass="30359.0" error="114.0" method="MALDI" evidence="1"/>
    <comment type="miscellaneous">
        <text>This enzyme couples pyrophosphate hydrolysis to the guanine ring-opening reaction. GTP cyclohydrolase and pyrophosphate phosphoydrolase activities probably occur at independent sites.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal-type GTP cyclohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.29"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64318">
        <property type="entry name" value="B64318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869640.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2QV6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D=1-268"/>
    </dbReference>
    <dbReference type="PDBsum" id="2QV6"/>
    <dbReference type="AlphaFoldDB" id="Q57609"/>
    <dbReference type="SMR" id="Q57609"/>
    <dbReference type="STRING" id="243232.MJ_0145"/>
    <dbReference type="EnsemblBacteria" id="AAB98128">
        <property type="protein sequence ID" value="AAB98128"/>
        <property type="gene ID" value="MJ_0145"/>
    </dbReference>
    <dbReference type="GeneID" id="1450989"/>
    <dbReference type="KEGG" id="mja:MJ_0145"/>
    <dbReference type="eggNOG" id="arCOG04202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080076_0_0_2"/>
    <dbReference type="InParanoid" id="Q57609"/>
    <dbReference type="OMA" id="VQIAHID"/>
    <dbReference type="OrthoDB" id="56576at2157"/>
    <dbReference type="PhylomeDB" id="Q57609"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14598"/>
    <dbReference type="BRENDA" id="3.5.4.29">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57609"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043740">
        <property type="term" value="F:GTP cyclohydrolase IIa activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00608">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007839">
        <property type="entry name" value="GTP_CycHdrlase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202">
        <property type="entry name" value="PTHR42202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05165">
        <property type="entry name" value="GCH_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009265">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145754" description="GTP cyclohydrolase III">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decrease in GTP cyclohydrolase and pyrophosphate phosphohydrolase activities." evidence="1">
        <original>H</original>
        <variation>Q</variation>
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="12"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="18"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="24"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="54"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="94"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="120"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="132"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="178"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="184"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="241"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="12475257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2QV6"/>
        </source>
    </evidence>
    <sequence length="268" mass="30286" checksum="627BBD523868B05B" modified="1998-12-15" version="2">MIQITVIQIDNYGPWTVTPNPRRESDLQALQSRLYADLNLMFGAHKGLVFYTRFDNLIAITNGIDLITHKRIQESIRNRYPFTVSMVIASAETPYEAQKLATETLQEYGSAQDENRKEVLDVANELVVDGYVQIAHIDINNITGTLTDIVSAYDTYLNVNKVKLALMEELLKYNALLFFIGGDNFMAPSNGMSEEDFLDIFNRINKKYKIELKAGIGIGRTAEDASNLADIGLEKIRGKLVDKNVCTLKQDDFLESKMGMGKIYHPQF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q57632</accession>
    <name>HJA1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable archaeal histone 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0168</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or heterodimer with another histone. Dimers then assemble into higher oligomers, with the DNA wrapped around the protein core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64321">
        <property type="entry name" value="A64321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869663.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57632"/>
    <dbReference type="SMR" id="Q57632"/>
    <dbReference type="STRING" id="243232.MJ_0168"/>
    <dbReference type="EnsemblBacteria" id="AAB98153">
        <property type="protein sequence ID" value="AAB98153"/>
        <property type="gene ID" value="MJ_0168"/>
    </dbReference>
    <dbReference type="GeneID" id="8803940"/>
    <dbReference type="KEGG" id="mja:MJ_0168"/>
    <dbReference type="eggNOG" id="arCOG02144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="InParanoid" id="Q57632"/>
    <dbReference type="OMA" id="CKHAGRK"/>
    <dbReference type="PhylomeDB" id="Q57632"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154987" description="Probable archaeal histone 1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P19267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="67" mass="7384" checksum="103E8B391D9C927C" modified="1997-11-01" version="1">MAELPVAPFERILKKAGAERVSRAAAEYLAEAVEEIALEIAKEAVELAKHAKRKTVKVEDIKLALKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57684</accession>
    <name>Y231_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0231</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0231</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64328">
        <property type="entry name" value="H64328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869729.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57684"/>
    <dbReference type="SMR" id="Q57684"/>
    <dbReference type="STRING" id="243232.MJ_0231"/>
    <dbReference type="EnsemblBacteria" id="AAB98219">
        <property type="protein sequence ID" value="AAB98219"/>
        <property type="gene ID" value="MJ_0231"/>
    </dbReference>
    <dbReference type="GeneID" id="1451084"/>
    <dbReference type="KEGG" id="mja:MJ_0231"/>
    <dbReference type="eggNOG" id="arCOG00322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_4_2_2"/>
    <dbReference type="InParanoid" id="Q57684"/>
    <dbReference type="OMA" id="LYRKSTF"/>
    <dbReference type="OrthoDB" id="49762at2157"/>
    <dbReference type="PhylomeDB" id="Q57684"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106752" description="Uncharacterized protein MJ0231">
        <location>
            <begin position="1"/>
            <end position="416"/>
        </location>
    </feature>
    <sequence length="416" mass="46661" checksum="48C4CC8AA0DAEEBA" modified="1996-11-01" version="1">MDLEKLIKIGEKEGFETEVLFVKSYEVSVDLDGKSVDSFQTGISYGIGVRVIKDGKVGFAYANKFDENIVYKAMKNLVEDKYTEFAHPQKYKEPKGMFYKEILDLDEEKLLEDLITMRDIALDNNAIVLSGGVSKEVGYARLINSNGVDVEEQDTYFSAAISIMYDGETSYECRTRHNIFDVEEISYRALDLAKKSANGKAISYKGNIVLSPRALYDLLSYTLMPAFSAENVQRDRSVLKGKIGEQIFGENITIIDDGTLDYALYSSKCDGEGTATQKTVLVENGVLKNYLYDIKRANREGKTSTGNASRGYRSLPYVSPTNFIIKETKNSLDDFDEYVYINGVIGSHTSNPITGDFAVEIQNSYYYKNGKIIPIKRGMFGGNIFEMFKEAIPLNDVEQRGKLISPSVVFKGEIIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57691</accession>
    <name>Y239_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0239</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0239</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64329">
        <property type="entry name" value="H64329"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57691"/>
    <dbReference type="STRING" id="243232.MJ_0239"/>
    <dbReference type="EnsemblBacteria" id="AAB98228">
        <property type="protein sequence ID" value="AAB98228"/>
        <property type="gene ID" value="MJ_0239"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0239"/>
    <dbReference type="eggNOG" id="arCOG05026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1590906_0_0_2"/>
    <dbReference type="OMA" id="FITARIN"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106756" description="Uncharacterized protein MJ0239">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <sequence length="164" mass="19548" checksum="4E62BF73D6E64389" modified="1996-11-01" version="1">MKNMDVYEILYQFCLEYEVLLDDEKIPLWKLKKEDLDKVDLDLPWTSIRDLAIYLYELKKKQQNSKELIKCDIVEILVGIALLKPEEGSNYMGLVTEDMCLTYLSELITARINCIARYYYMMKKPQNTNIFDEIILKFPQKKDIRASNINDLRELVGKIRNYFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57699</accession>
    <name>FER5_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0251</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0251</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64331">
        <property type="entry name" value="D64331"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869749.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57699"/>
    <dbReference type="STRING" id="243232.MJ_0251"/>
    <dbReference type="EnsemblBacteria" id="AAB98238">
        <property type="protein sequence ID" value="AAB98238"/>
        <property type="gene ID" value="MJ_0251"/>
    </dbReference>
    <dbReference type="GeneID" id="1451105"/>
    <dbReference type="KEGG" id="mja:MJ_0251"/>
    <dbReference type="eggNOG" id="arCOG02461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1745537_0_0_2"/>
    <dbReference type="InParanoid" id="Q57699"/>
    <dbReference type="OMA" id="VVVKYPY"/>
    <dbReference type="OrthoDB" id="96045at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <keyword id="KW-0408">Iron</keyword>
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    <sequence length="147" mass="17654" checksum="B01B3945B736D072" modified="1996-11-01" version="1">MENLEKKIELLKKIREFLILNLEIKKLMQELNVDSDIYEAYEKVTKIVREPNIKLYRQYYDAIKEMFYEEYGKKRKDISWYPKIDYNRCKNCEKCISFCPRGVYDAENGKVVVKYPYSCIVNCNACSIMCCENNAIIFPDEKIPRRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q57727</accession>
    <name>DGGGP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="1">DGGGP synthase</shortName>
            <shortName evidence="1">DGGGPS</shortName>
            <ecNumber evidence="1">2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0279</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB98267.1"/>
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        <property type="resolution" value="2.30 A"/>
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        <property type="resolution" value="2.90 A"/>
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        <property type="resolution" value="3.32 A"/>
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        <property type="entry name" value="UbiA_prenyltransferase"/>
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        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106770" description="Digeranylgeranylglyceryl phosphate synthase">
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            <begin position="1"/>
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        </location>
    </feature>
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    </feature>
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    </feature>
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    <feature type="helix" evidence="2">
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    <evidence type="ECO:0000255" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="6M31"/>
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    </evidence>
    <sequence length="283" mass="31754" checksum="9C98641137661413" modified="1996-11-01" version="1">MGVFMEKLKTYLELIRVKNCITASIGGIIGYLISSNFEIDILKSLLVFFVVFFVCAYGNVINDIFDIEIDRINKPSRPLPSGKIKLNEAKKFSAILLILGLVLSLFINIYALIIAVINALFLYLYAKKYKKYKPIGNFIIGYLTGSVFLFGGVAGKNVMPVVILFLCSLLSIWGREIVKDFEDMEGDKKEGVISLPIKYGKKSLYFATFLVVLAVILSPLPYILKIFGIWYLILIAICDILFIYAMALLLKEPNKETASKVSKFLKIIMNIVLLAFIVGAIKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57728</accession>
    <name>NAC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nac</name>
        <name type="ordered locus">MJ0280</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAC-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64335">
        <property type="entry name" value="A64335"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869778.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57728"/>
    <dbReference type="SMR" id="Q57728"/>
    <dbReference type="STRING" id="243232.MJ_0280"/>
    <dbReference type="EnsemblBacteria" id="AAB98268">
        <property type="protein sequence ID" value="AAB98268"/>
        <property type="gene ID" value="MJ_0280"/>
    </dbReference>
    <dbReference type="GeneID" id="1451135"/>
    <dbReference type="KEGG" id="mja:MJ_0280"/>
    <dbReference type="eggNOG" id="arCOG04061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146475_1_0_2"/>
    <dbReference type="InParanoid" id="Q57728"/>
    <dbReference type="OMA" id="PRKMKQM"/>
    <dbReference type="OrthoDB" id="105165at2157"/>
    <dbReference type="PhylomeDB" id="Q57728"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="HYPK_UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="SSF46934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00264">
        <property type="entry name" value="TIGR00264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000135601" description="Nascent polypeptide-associated complex protein">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="NAC-A/B" evidence="1">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <sequence length="128" mass="14958" checksum="CE6B571273455E8D" modified="1996-11-01" version="1">MFPGKVNPRMLKKMQKMMKDFGMETEDLDVRKVIFVFDDEEWVFEEPKVQVMDILGVKTYSITGKPKKIKKEKVEEEEEVKVEITEEDVELVAKQCNVSKEEARKALEECNGDIAEAILKLEEEKEEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57742</accession>
    <name>HELX_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-dependent helicase MJ0294</fullName>
            <ecNumber>3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0294</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64336">
        <property type="entry name" value="G64336"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869792.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57742"/>
    <dbReference type="SMR" id="Q57742"/>
    <dbReference type="STRING" id="243232.MJ_0294"/>
    <dbReference type="PRIDE" id="Q57742"/>
    <dbReference type="EnsemblBacteria" id="AAB98279">
        <property type="protein sequence ID" value="AAB98279"/>
        <property type="gene ID" value="MJ_0294"/>
    </dbReference>
    <dbReference type="GeneID" id="1451149"/>
    <dbReference type="KEGG" id="mja:MJ_0294"/>
    <dbReference type="eggNOG" id="arCOG00557">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002025_0_0_2"/>
    <dbReference type="InParanoid" id="Q57742"/>
    <dbReference type="OMA" id="VVDEWHE"/>
    <dbReference type="OrthoDB" id="995at2157"/>
    <dbReference type="PhylomeDB" id="Q57742"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013701">
        <property type="entry name" value="DEAD/DEAH_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017170">
        <property type="entry name" value="Lhr-like_ATP-dep_RNA_helic_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045628">
        <property type="entry name" value="Lhr_WH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08494">
        <property type="entry name" value="DEAD_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19306">
        <property type="entry name" value="Lhr_WH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037307">
        <property type="entry name" value="Lhr-like_helic_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102196" description="Uncharacterized ATP-dependent helicase MJ0294">
        <location>
            <begin position="1"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="43"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="266"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEVH box">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="56"/>
            <end position="63"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="841" mass="97268" checksum="4C28AA8AA9459505" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q57763</accession>
    <name>SPEH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName>AdoMetDC</shortName>
            <shortName>SAMDC</shortName>
            <ecNumber>4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">speH</name>
        <name type="ordered locus">MJ0315</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="6667" last="6672">
            <title>S-adenosylmethionine decarboxylase from the archaeon Methanococcus jannaschii: identification of a novel family of pyruvoyl enzymes.</title>
            <authorList>
                <person name="Kim A.D."/>
                <person name="Graham D.E."/>
                <person name="Seeholzer S.H."/>
                <person name="Markham G.D."/>
            </authorList>
            <dbReference type="PubMed" id="11073910"/>
            <dbReference type="DOI" id="10.1128/JB.182.23.6667-6672.2000"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>SELF-PROCESSING</scope>
        <scope>CLEAVAGE SITE</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>PYRUVATE FORMATION AT SER-64</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="265" last="273">
            <title>Catalytic properties of the archaeal S-adenosylmethionine decarboxylase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Lu Z.J."/>
                <person name="Markham G.D."/>
            </authorList>
            <dbReference type="PubMed" id="14573607"/>
            <dbReference type="DOI" id="10.1074/jbc.M308793200"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SCHIFF BASE FORMATION</scope>
        <scope>CATALYTIC MECHANISM</scope>
    </reference>
    <comment type="function">
        <text>Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + S-adenosyl-L-methionine = CO2 + S-adenosyl 3-(methylsulfanyl)propylamine</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="2">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Competitively inhibited by methylglyoxal bis-guanylhydrazone. Inactivated by treatment with the imine reductant NaCNBH(3) only in the presence of substrate.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">95 uM for S-adenosyl-L-methionine (at pH 7.5 and 22 degrees Celsius)</KM>
            <Vmax evidence="3">37.0 nmol/min/mg enzyme (at pH 7.5 and 22 degrees Celsius)</Vmax>
            <text>The turnover number increases 30-fold when temperature is increased from 22 to 70 degrees Celsius, whereas the Km increases 3-fold.</text>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 6-8.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is superior to 90 degrees Celsius. Thermostable. Retains full activity after 3 hours at 70 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="2">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="mass spectrometry" mass="6793.1" method="MALDI" evidence="2">
        <molecule>S-adenosylmethionine decarboxylase beta chain</molecule>
    </comment>
    <comment type="mass spectrometry" mass="6990.2" method="MALDI" evidence="2">
        <molecule>S-adenosylmethionine decarboxylase alpha chain</molecule>
        <text>Pyruvoyl group-containing alpha subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98301" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.1.1.50"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98301.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64339">
        <property type="entry name" value="D64339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496461.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57763"/>
    <dbReference type="SMR" id="Q57763"/>
    <dbReference type="STRING" id="243232.MJ_0315"/>
    <dbReference type="EnsemblBacteria" id="AAB98301">
        <property type="protein sequence ID" value="AAB98301"/>
        <property type="gene ID" value="MJ_0315"/>
    </dbReference>
    <dbReference type="GeneID" id="1451170"/>
    <dbReference type="KEGG" id="mja:MJ_0315"/>
    <dbReference type="eggNOG" id="arCOG00279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125470_2_3_2"/>
    <dbReference type="InParanoid" id="Q57763"/>
    <dbReference type="OMA" id="VYTCGEH"/>
    <dbReference type="OrthoDB" id="106151at2157"/>
    <dbReference type="PhylomeDB" id="Q57763"/>
    <dbReference type="SABIO-RK" id="Q57763"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006557">
        <property type="term" value="P:S-adenosylmethioninamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042286">
        <property type="entry name" value="AdoMetDC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042284">
        <property type="entry name" value="AdoMetDC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="PTHR33866"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="SSF56276"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000030133" description="S-adenosylmethionine decarboxylase beta chain">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030134" description="S-adenosylmethionine decarboxylase alpha chain">
        <location>
            <begin position="64"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="1">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="4">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis">
        <location>
            <begin position="63"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="2">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11073910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="14573607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="124" mass="13792" checksum="BC1152CDE080F113" modified="2001-08-14" version="2" precursor="true">MLKYLGKHLILELWGCDPKALDDIEGIEKMLVDSVKACGATLICVRTHKFSPQGATGVAVLAESHIAIHTYPEYGYAALDVFTCGEHTDPYKALEVIREFLKPKSIQIIDLKRGLMENGTFELK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57772</accession>
    <name>Y326_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative permease MJ0326</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0326</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64340">
        <property type="entry name" value="F64340"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57772"/>
    <dbReference type="STRING" id="243232.MJ_0326"/>
    <dbReference type="EnsemblBacteria" id="AAB98314">
        <property type="protein sequence ID" value="AAB98314"/>
        <property type="gene ID" value="MJ_0326"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0326"/>
    <dbReference type="eggNOG" id="arCOG02807">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024508_0_1_2"/>
    <dbReference type="InParanoid" id="Q57772"/>
    <dbReference type="OMA" id="RKGSYFF"/>
    <dbReference type="PhylomeDB" id="Q57772"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015853">
        <property type="term" value="P:adenine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015854">
        <property type="term" value="P:guanine transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="PTHR43337"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000106795" description="Putative permease MJ0326">
        <location>
            <begin position="1"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="345"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="416"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="436" mass="46723" checksum="409B4075F6D70891" modified="1996-11-01" version="1">MIMKFVEKYFEFEKYGTNLKVETLAGITTFMTMAYIIFVNPQILSTAGMDFGAVMVATCIASAIATLVMGLYARYPFALAPGMGLNAYFTYGVCLGMGIDWRVALGAVFISGVLFIILTLTKIRTWIFNVIPNAIKYGTAVGIGLFIAFIGLKSAGIIVSSKATLVTLGNLMEPSTLLALFGIFLTSILVSRNVIGAILIGIIVTSLIGMILGISPFPEGIFSMPPSIAPTFLQLDIMGALNLGLLTIVLAFFFVDMFDTLGTLSALASQAGYLDKDGKLPRVEKALMADATGTVVGSLLGTSTVTTYIESASGIALGGRTGFVSVVVAMLFLLSLFFYPVVKAIPPYATAAALVIVGALMMRSVKYIDFDDYTEAIPAFITLLTIPLTFSIATGLALGFITYPILKVFTGRWKEVHWLVYVLAVIFALRFVYLSG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q57793</accession>
    <name>Y347_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0347</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0347</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64343">
        <property type="entry name" value="C64343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869845.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57793"/>
    <dbReference type="SMR" id="Q57793"/>
    <dbReference type="STRING" id="243232.MJ_0347"/>
    <dbReference type="EnsemblBacteria" id="AAB98331">
        <property type="protein sequence ID" value="AAB98331"/>
        <property type="gene ID" value="MJ_0347"/>
    </dbReference>
    <dbReference type="GeneID" id="1451203"/>
    <dbReference type="KEGG" id="mja:MJ_0347"/>
    <dbReference type="HOGENOM" id="CLU_1727253_0_0_2"/>
    <dbReference type="InParanoid" id="Q57793"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106817" description="Uncharacterized protein MJ0347">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <sequence length="151" mass="16627" checksum="B620EC8566457E37" modified="1996-11-01" version="1">MGFDPVTFAKIKKISIDSNADGYIDYANQAGNADKLDGKDASDFLSIDSFKDLKEDNGYIKFPTGLIMQWGKVYVNNTTSYQEIPVNFTIEFPNKCLNVFASIYEGSGVDNDYIKCITRIANITTKGCVVCITDISGASGDVGFFWFAIGY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q57810</accession>
    <name>Y364_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0364</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0364</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64345">
        <property type="entry name" value="D64345"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57810"/>
    <dbReference type="STRING" id="243232.MJ_0364"/>
    <dbReference type="EnsemblBacteria" id="AAB98355">
        <property type="protein sequence ID" value="AAB98355"/>
        <property type="gene ID" value="MJ_0364"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0364"/>
    <dbReference type="HOGENOM" id="CLU_2115496_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106833" description="Uncharacterized protein MJ0364">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <sequence length="114" mass="13315" checksum="87C1148084DF542F" modified="1996-11-01" version="1">MGSMIEINFSHDYFKLREQRFTTIRSKHYLKNKGLRVGDVVKIKHPSGEFPAKIVGVEVKRICDIPLEILKKDAEFEGFNIKTHQDFVDLLNSFIPYKSNKLTTEKAIIYLERV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q57812</accession>
    <name>Y366_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0366</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0366</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64345">
        <property type="entry name" value="F64345"/>
    </dbReference>
    <dbReference type="PDB" id="2EFV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-92"/>
    </dbReference>
    <dbReference type="PDBsum" id="2EFV"/>
    <dbReference type="AlphaFoldDB" id="Q57812"/>
    <dbReference type="BMRB" id="Q57812"/>
    <dbReference type="SMR" id="Q57812"/>
    <dbReference type="STRING" id="243232.MJ_0366"/>
    <dbReference type="EnsemblBacteria" id="AAB98357">
        <property type="protein sequence ID" value="AAB98357"/>
        <property type="gene ID" value="MJ_0366"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0366"/>
    <dbReference type="eggNOG" id="arCOG05030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188099_0_0_2"/>
    <dbReference type="OMA" id="LNCELVK"/>
    <dbReference type="PhylomeDB" id="Q57812"/>
    <dbReference type="EvolutionaryTrace" id="Q57812"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024254">
        <property type="entry name" value="DUF2540"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10802">
        <property type="entry name" value="DUF2540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106835" description="Uncharacterized protein MJ0366">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="41"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="54"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="75"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="2EFV"/>
        </source>
    </evidence>
    <sequence length="92" mass="10854" checksum="CCB9B3E16BAF403A" modified="1996-11-01" version="1">MPLVGFMKEKKRATFYLYKNIDGRKLRYLLHKLENVENVDIDTLRRAIEAEKKYKRSITLTEEEEVIIQRLGKSANLLLNCELVKLDEGERA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57819</accession>
    <name>Y374_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0374</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0374</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64346">
        <property type="entry name" value="F64346"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57819"/>
    <dbReference type="SMR" id="Q57819"/>
    <dbReference type="STRING" id="243232.MJ_0374"/>
    <dbReference type="EnsemblBacteria" id="AAB98363">
        <property type="protein sequence ID" value="AAB98363"/>
        <property type="gene ID" value="MJ_0374"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0374"/>
    <dbReference type="eggNOG" id="arCOG03056">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03118">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777588_0_0_2"/>
    <dbReference type="InParanoid" id="Q57819"/>
    <dbReference type="OMA" id="PYKVIAW"/>
    <dbReference type="PhylomeDB" id="Q57819"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106837" description="Uncharacterized protein MJ0374">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <sequence length="330" mass="37018" checksum="BA84B0170694C097" modified="1997-11-01" version="1">MISGGYMDLYAMAEYLVNNYGYIGIFIISFTEAFIQPIPPDVFIIGASFFGLNPIISAIVATIGTTLGGLFGYFLGDKLGHPIFIKLFGEKYLHKGEEFFNKYGVYGVVIAGFSPLPYKVIAWLSGIFEMHKLLFTVGTIIGRLPRFLAVAYFGDVLGNINRLSDINIYLFYLINSHYNYIFDAIMPIISKTAYPLIAITSLIIFIKNRKFGMKLIFALFLAFMIAFSLKYLVNEPRPYLVLDNVHLLCNEGNEPSFPSGHTTLAFTLATSLLFYSKKLGILFLSWAIIVAYSRVYVGVHYPLDVLAGMIIGIFCGCLTRIDIYKLIDNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57827</accession>
    <name>CAS5_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated protein Cas5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas5</name>
        <name type="ordered locus">MJ0382</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas5 family. Subtype I-A/Apern subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64347">
        <property type="entry name" value="F64347"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57827"/>
    <dbReference type="STRING" id="243232.MJ_0382"/>
    <dbReference type="EnsemblBacteria" id="AAB98378">
        <property type="protein sequence ID" value="AAB98378"/>
        <property type="gene ID" value="MJ_0382"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0382"/>
    <dbReference type="eggNOG" id="arCOG02672">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1056094_0_0_2"/>
    <dbReference type="OMA" id="MRREYTF"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021124">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013422">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010153">
        <property type="entry name" value="CRISPR-assoc_prot_Cas5a-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09704">
        <property type="entry name" value="Cas_Cas5d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01874">
        <property type="entry name" value="cas_cas5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02593">
        <property type="entry name" value="CRISPR_cas5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106845" description="CRISPR-associated protein Cas5">
        <location>
            <begin position="1"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="245" mass="28255" checksum="6169A4FFC51AE8BA" modified="1996-11-01" version="1">MIMEALVALLRFPFYSFGKQSYQVRQSLLLPSPSALKGALAKGIILLGGKKGKSLDEIAKKTVEELENKLIYVGAKPYKSSIIKTPILLKRLRNLEDQKNPEKSDAMRREYVFAREILAIYVFRRKLSEEEKDLMLKAVYLIDQLGDTECVGNVIKTEWVEVKEEKAPLKTYVKFKKVSKMSINGGIIENMLETPNFGNKVKEEPYLLPLIEKRYRRSSYYVESDRIFDGNYKIIAFDEVIGLWI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q57863</accession>
    <name>Y420_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0420</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0420</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64352">
        <property type="entry name" value="D64352"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57863"/>
    <dbReference type="STRING" id="243232.MJ_0420"/>
    <dbReference type="EnsemblBacteria" id="AAB98408">
        <property type="protein sequence ID" value="AAB98408"/>
        <property type="gene ID" value="MJ_0420"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0420"/>
    <dbReference type="eggNOG" id="arCOG03207">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_711001_0_0_2"/>
    <dbReference type="OMA" id="CEIGINY"/>
    <dbReference type="PhylomeDB" id="Q57863"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002760">
        <property type="entry name" value="O_anti_polymase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01901">
        <property type="entry name" value="O_anti_polymase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106866" description="Uncharacterized protein MJ0420">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="380" mass="43660" checksum="77E6B309C63B41B1" modified="1996-11-01" version="1">MHICNIIEKIKKIDLFHPVSIVIIGHLMIFILAFPYLDDIGLYSLFKILGVLSIFIVGFFLPFIFPMQIKFNRHILYLSFLLLSFLSIFGAFKITHSLFLSIAYLLFILIIAELFVKFYKKKIFVDILFSIGIIAFLLIVLIYGAIPLFNYEVRMTINSEPLRLISMGALIYAGIENKVYFIIAFMILVLLGYKAGVLMLFIAYIIYRYRNILFKYMVLLAFALLIFLGIMGKIILLSSNQNWDLNPIELLCYRAYFDLYVLSKIVESNILTLGKITLTPNGEHFIGELLFKYPHNITTTLFGTIYLDFGIFGGLFAMLLGVISKYIYEGDKKLYAIYASLLLAYCEIGINYGFLVVLSLLLYINAKLVFAKLLKLINEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57874</accession>
    <name>Y432_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0432</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0432</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64353">
        <property type="entry name" value="H64353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869931.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57874"/>
    <dbReference type="SMR" id="Q57874"/>
    <dbReference type="STRING" id="243232.MJ_0432"/>
    <dbReference type="EnsemblBacteria" id="AAB98421">
        <property type="protein sequence ID" value="AAB98421"/>
        <property type="gene ID" value="MJ_0432"/>
    </dbReference>
    <dbReference type="GeneID" id="1451292"/>
    <dbReference type="KEGG" id="mja:MJ_0432"/>
    <dbReference type="eggNOG" id="arCOG00732">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142189_1_1_2"/>
    <dbReference type="InParanoid" id="Q57874"/>
    <dbReference type="OMA" id="RTAFEGH"/>
    <dbReference type="OrthoDB" id="121646at2157"/>
    <dbReference type="PhylomeDB" id="Q57874"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027395">
        <property type="entry name" value="WH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13601">
        <property type="entry name" value="HTH_34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106873" description="Uncharacterized protein MJ0432">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <sequence length="92" mass="10768" checksum="FA5EDD9849A0DFB2" modified="1996-11-01" version="1">MKIFNSVVRVKILALLYGLEYCEFNYLKEKLNLTDGNLEHHLKKLEECGFVETKKSVIKGRVKTIIKITNKGRVAFKNYIYEILQLSKNIEC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57875</accession>
    <name>Y433_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0433</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0433</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64354">
        <property type="entry name" value="A64354"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869932.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57875"/>
    <dbReference type="SMR" id="Q57875"/>
    <dbReference type="STRING" id="243232.MJ_0433"/>
    <dbReference type="EnsemblBacteria" id="AAB98433">
        <property type="protein sequence ID" value="AAB98433"/>
        <property type="gene ID" value="MJ_0433"/>
    </dbReference>
    <dbReference type="GeneID" id="1451293"/>
    <dbReference type="KEGG" id="mja:MJ_0433"/>
    <dbReference type="eggNOG" id="arCOG09667">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1458216_0_0_2"/>
    <dbReference type="OMA" id="CYFDKIF"/>
    <dbReference type="OrthoDB" id="103360at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106874" description="Uncharacterized protein MJ0433">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <sequence length="175" mass="20494" checksum="54DFCBA805D0EDAE" modified="1996-11-01" version="1">MDAKEILELVEESYKSEDGDYKNKVYFISYFLSSLIFVLIHISIKYWNFNILFIVSLLLIIGSILIVRQQKLYKKTRCYFDKIFEKIVKYGMIAVVLSSVITLYTYPRISGVAIAGIFGFLLVIDGILFKSKKRKFLGLLMMFSSIPMFIFHEYQFLIFAFVQFLVALCFLICKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q57915</accession>
    <name>Y492_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0492</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0492</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64361">
        <property type="entry name" value="D64361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869993.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57915"/>
    <dbReference type="STRING" id="243232.MJ_0492"/>
    <dbReference type="EnsemblBacteria" id="AAB98488">
        <property type="protein sequence ID" value="AAB98488"/>
        <property type="gene ID" value="MJ_0492"/>
    </dbReference>
    <dbReference type="GeneID" id="1451354"/>
    <dbReference type="KEGG" id="mja:MJ_0492"/>
    <dbReference type="eggNOG" id="arCOG08284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353214_0_0_2"/>
    <dbReference type="OMA" id="IMKSENT"/>
    <dbReference type="OrthoDB" id="128645at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106897" description="Uncharacterized protein MJ0492">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="95" mass="10695" checksum="7A49FD4F888A365C" modified="1996-11-01" version="1">MNFVIIIAILLLGISLILAFTVLNKSKSKTTMAYKRAQEEKIDTEIKMLKNLKNNVCSGASDEIIDNILNSENNILKEALKNNLDDADVCKKLRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57948</accession>
    <name>EHAA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable [NiFe]-hydrogenase-type-3 Eha complex membrane subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ehaA</name>
        <name type="ordered locus">MJ0528</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the integral membrane subunits of multisubunit membrane-bound [NiFe]-hydrogenase eha. Eha is predicted to form large electron transfer complex and might catalyze energy-driven reduction of low-potential redox carriers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Putative multisubunit membrane-bound [NiFe]-hydrogenase eha is composed of at least 20 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EhaA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64365">
        <property type="entry name" value="H64365"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57948"/>
    <dbReference type="STRING" id="243232.MJ_0528"/>
    <dbReference type="EnsemblBacteria" id="AAB98519">
        <property type="protein sequence ID" value="AAB98519"/>
        <property type="gene ID" value="MJ_0528"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0528"/>
    <dbReference type="eggNOG" id="arCOG05035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174516_1_0_2"/>
    <dbReference type="OMA" id="MKLSFDM"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011306">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17367">
        <property type="entry name" value="NiFe_hyd_3_EhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005019">
        <property type="entry name" value="EhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000138112" description="Probable [NiFe]-hydrogenase-type-3 Eha complex membrane subunit A">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="98" mass="10936" checksum="B024D42F4239FE96" modified="1996-11-01" version="1">MVYNNSLLVRVMDIIILYLIALITSVIVALVLKLPIIPKEKPIRFSFETSIIFPTPILALGIEAIFRNLFGDYISLAFFAGLFGALLSKYADKLFGEP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q57949</accession>
    <name>Y529_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0529</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0529</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64366">
        <property type="entry name" value="A64366"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57949"/>
    <dbReference type="SMR" id="Q57949"/>
    <dbReference type="STRING" id="243232.MJ_0529"/>
    <dbReference type="EnsemblBacteria" id="AAB98520">
        <property type="protein sequence ID" value="AAB98520"/>
        <property type="gene ID" value="MJ_0529"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0529"/>
    <dbReference type="eggNOG" id="arCOG05036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1297489_0_0_2"/>
    <dbReference type="OMA" id="PIYSIKK"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000149790" description="Uncharacterized HTH-type transcriptional regulator MJ0529">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="39"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00257"/>
        </source>
    </evidence>
    <sequence length="232" mass="27091" checksum="46A162ADFDE60EF1" modified="1996-11-01" version="1">MAEYVFLIYLVDIKYQFRENMYKKLEIIERAILLNPQYIQAFREKLKITQSKLAKESGISQSHLSMLEKGKRPATKLIATAVTLGLLKCFSSNNVENPIIELLDALSLLKFEDTFAEFVSEIIEKGDKGYLRLIENYPVLIISKENLLNEMRSRLEVMDIERIELSRGRIKVIGKHLDNKYVEIFLDCSDIRRLEKKFMKKTGKKVIIQVFPKDEVPPIYSINKDCVIIHCW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57973</accession>
    <name>Y553_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Acylphosphatase-like protein MJ0553</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0553</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64369">
        <property type="entry name" value="A64369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870057.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57973"/>
    <dbReference type="SMR" id="Q57973"/>
    <dbReference type="STRING" id="243232.MJ_0553"/>
    <dbReference type="EnsemblBacteria" id="AAB98549">
        <property type="protein sequence ID" value="AAB98549"/>
        <property type="gene ID" value="MJ_0553"/>
    </dbReference>
    <dbReference type="GeneID" id="1451418"/>
    <dbReference type="KEGG" id="mja:MJ_0553"/>
    <dbReference type="eggNOG" id="arCOG01674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656874_0_0_2"/>
    <dbReference type="InParanoid" id="Q57973"/>
    <dbReference type="OMA" id="VYNHKDG"/>
    <dbReference type="OrthoDB" id="109619at2157"/>
    <dbReference type="PhylomeDB" id="Q57973"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="PTHR47268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158562" description="Acylphosphatase-like protein MJ0553">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <sequence length="153" mass="17880" checksum="3AC47E49C620686B" modified="1996-11-01" version="1">MITTYELIIYGRVQHVGFRDRIEHIGRGLGISGVVYNHKDGTVRILANFDDEEIKELFKKSIKALEKKDKLIKIEKIEEKELNAYIEFPEGISRLSSDDILELNKKLDEGVKYIKLIFSELEEHKKILLDIKDTQIKTIKVLNEIKELLEKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58027</accession>
    <name>SPCS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>O-phosphoseryl-tRNA(Sec) selenium transferase</fullName>
            <ecNumber evidence="2">2.9.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Selenocysteine synthase</fullName>
            <shortName>Sec synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Selenocysteinyl-tRNA(Sec) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Sep-tRNA:Sec-tRNA synthase</fullName>
            <shortName>SepSecS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">spcS</name>
        <name type="ordered locus">MJ0610</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Proc. Natl. Acad. Sci. U.S.A." volume="103" first="18923" last="18927">
            <title>RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea.</title>
            <authorList>
                <person name="Yuan J."/>
                <person name="Palioura S."/>
                <person name="Salazar J.C."/>
                <person name="Su D."/>
                <person name="O'Donoghue P."/>
                <person name="Hohn M.J."/>
                <person name="Cardoso A.M."/>
                <person name="Whitman W.B."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="17142313"/>
            <dbReference type="DOI" id="10.1073/pnas.0609703104"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>PYRIDOXAL PHOSPHATE AT LYS-277</scope>
        <scope>MUTAGENESIS OF LYS-277</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + O-phospho-L-seryl-tRNA(Sec) + selenophosphate = L-selenocysteinyl-tRNA(Sec) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:25041"/>
            <dbReference type="Rhea" id="RHEA-COMP:9743"/>
            <dbReference type="Rhea" id="RHEA-COMP:9947"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:78573"/>
            <dbReference type="EC" id="2.9.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SepSecS family.</text>
    </comment>
    <dbReference type="EC" id="2.9.1.2" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64376">
        <property type="entry name" value="B64376"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870114.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58027"/>
    <dbReference type="SMR" id="Q58027"/>
    <dbReference type="STRING" id="243232.MJ_0610"/>
    <dbReference type="EnsemblBacteria" id="AAB98603">
        <property type="protein sequence ID" value="AAB98603"/>
        <property type="gene ID" value="MJ_0610"/>
    </dbReference>
    <dbReference type="GeneID" id="1451475"/>
    <dbReference type="KEGG" id="mja:MJ_0610"/>
    <dbReference type="eggNOG" id="arCOG00119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022508_0_0_2"/>
    <dbReference type="InParanoid" id="Q58027"/>
    <dbReference type="OMA" id="HIINGAY"/>
    <dbReference type="OrthoDB" id="16805at2157"/>
    <dbReference type="BRENDA" id="2.9.1.2">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00898"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098621">
        <property type="term" value="F:phosphoseryl-selenocysteinyl-tRNA selenium transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019872">
        <property type="entry name" value="Sec-tRNA_Se_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12944">
        <property type="entry name" value="PTHR12944"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017689">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03531">
        <property type="entry name" value="selenium_SpcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0711">Selenium</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000219881" description="O-phosphoseryl-tRNA(Sec) selenium transferase">
        <location>
            <begin position="1"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Tetramerization" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphate loop (P-loop)" evidence="3">
        <location>
            <begin position="92"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylate" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2 4">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="4">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q6LZM9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q6P6M7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="17142313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="434" mass="48684" checksum="09AFBC5819613EF5" modified="1996-11-01" version="1">MGLNITGLIPKHMENRGKLTLKENLKIIENILEQRKAPENGIDEEHIKLLLRLLSFMDTDKDPNVVQIGEREARVYTKLQRDGVFDFCHGVGRSGNLIDPQPKAPGASVMYKLTNKLLESFLKALGLKVNAIATPVATGMSLALCLSAARKKYNSNVVIYPYAAHKSPIKATSFIGMRMRLVETVLDGDIVKVEVSDIEDAIRKEINENNNPVVLSTLTFFPPRKSDDIKEIAKICQDYDIPHIINGAYAIQNFYYIEKLKKALKYRIDAVVSSSDKNLFTPIGGGIIYTKDESFLKEISLTYPGRASANPIVNILISLLAIGTKDYLNLMKEQKECKKLLNELLEDLAKKKGEKVLNVENPISSCITTKKDPLDVAGKLYNLRVTGPRGVRRNDKFGTCYLKEYPYDYIVVNSAIGVKKEDIYKVIEKLDEVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58059</accession>
    <name>Y642_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0642</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0642</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64380">
        <property type="entry name" value="B64380"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870147.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58059"/>
    <dbReference type="SMR" id="Q58059"/>
    <dbReference type="STRING" id="243232.MJ_0642"/>
    <dbReference type="EnsemblBacteria" id="AAB98644">
        <property type="protein sequence ID" value="AAB98644"/>
        <property type="gene ID" value="MJ_0642"/>
    </dbReference>
    <dbReference type="GeneID" id="1451508"/>
    <dbReference type="KEGG" id="mja:MJ_0642"/>
    <dbReference type="eggNOG" id="arCOG03417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025776_0_0_2"/>
    <dbReference type="InParanoid" id="Q58059"/>
    <dbReference type="OMA" id="LIDWNIF"/>
    <dbReference type="OrthoDB" id="12936at2157"/>
    <dbReference type="PhylomeDB" id="Q58059"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106968" description="Uncharacterized protein MJ0642">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="45"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00344"/>
        </source>
    </evidence>
    <sequence length="333" mass="40006" checksum="1EF47CDE74593BDF" modified="1996-11-01" version="1">MITMALNFYKYYSRQIKYALHFNILNNSIIDAENKYINFQKPLKNVIVSDSMFIDTAYLLKIKKMIKSIKGVFWADFVLKKYYPLYSDYLKGKISNVGTDGKINYGEILNINPDMIFLIDWNIFNPLSKWLDKNNIPYTKTGNYKEPKFLGKMEWIKFYASFYNKYKKAEKVFNKIIEEKRRILKSLNSRSIKYKPVVAFFGYHKNQPYIYGKSHYIPNWIREIKGNYLFENVEGTNYHYIDRKIFNSRAKYADVCILDTMGEDIDIKELLKNNPHFLKFRAYKNKRFYITTKDYLKFETLKCSEVMEEYVKIIHPKIYQNGDNDLKYFIKVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58088</accession>
    <name>Y675_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0675</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0675</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64384">
        <property type="entry name" value="C64384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870180.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58088"/>
    <dbReference type="SMR" id="Q58088"/>
    <dbReference type="STRING" id="243232.MJ_0675"/>
    <dbReference type="EnsemblBacteria" id="AAB98669">
        <property type="protein sequence ID" value="AAB98669"/>
        <property type="gene ID" value="MJ_0675"/>
    </dbReference>
    <dbReference type="GeneID" id="1451541"/>
    <dbReference type="KEGG" id="mja:MJ_0675"/>
    <dbReference type="eggNOG" id="arCOG00913">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042160_0_0_2"/>
    <dbReference type="OMA" id="ECGGKKF"/>
    <dbReference type="OrthoDB" id="24160at2157"/>
    <dbReference type="PhylomeDB" id="Q58088"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014435">
        <property type="entry name" value="BpsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002723">
        <property type="entry name" value="BpsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01861">
        <property type="entry name" value="DUF43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005895">
        <property type="entry name" value="UCP005895_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106985" description="Uncharacterized protein MJ0675">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <sequence length="391" mass="44553" checksum="08DCBF6899EC9A6B" modified="1998-12-15" version="2">MKIIGKIGKGKVEVNEKTKFSILLNNVAKKADIAEGKRAVEDIIRVIYRHQPISTKKIAQKTRLPLPIVAKVRTILEREKILKRTERGAELTDLGKEFAENFLKLKYKKSLTCKTCNGRGIVLDEFFEDILNKVRVWAKRRPLVDTTIDQSFATPETSTYRAALMYERGDLEGKRILFVGDDDLTSLPTALTNMAEEIAVVDIDERILKLIEKFSQKEGVKIKTIKHDLRNPLPQDLKERFDVISTDPPYTVDGLKLFLSRGIEALGKEGIAYLSYSHKPIDEWLSIQKAITNMGFVISELIPNFNYYEGSEIIANTTFIARLVGKNLKINIGDTEKIYTGLVKPVIRYYKCLKCGKIHKVGEEVKKVEDLVCECGGKKFKMIKREKLKNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58156</accession>
    <name>Y746_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0746</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0746</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64393">
        <property type="entry name" value="B64393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870251.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58156"/>
    <dbReference type="SMR" id="Q58156"/>
    <dbReference type="STRING" id="243232.MJ_0746"/>
    <dbReference type="EnsemblBacteria" id="AAB98739">
        <property type="protein sequence ID" value="AAB98739"/>
        <property type="gene ID" value="MJ_0746"/>
    </dbReference>
    <dbReference type="GeneID" id="1451623"/>
    <dbReference type="KEGG" id="mja:MJ_0746"/>
    <dbReference type="eggNOG" id="arCOG01097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113705_0_0_2"/>
    <dbReference type="InParanoid" id="Q58156"/>
    <dbReference type="OMA" id="NFEGECK"/>
    <dbReference type="OrthoDB" id="104203at2157"/>
    <dbReference type="PhylomeDB" id="Q58156"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01046">
        <property type="entry name" value="Rubrerythrin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045236">
        <property type="entry name" value="Rubrerythrin-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135071" description="Uncharacterized protein MJ0746">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="Ferritin-like diiron" evidence="1">
        <location>
            <begin position="13"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00085"/>
        </source>
    </evidence>
    <sequence length="141" mass="15905" checksum="043E256886F34C07" modified="1996-11-01" version="1">MELDLINEHKIGVTKGTELEKEVQANFEGECKEVGLYLAMARQAQREGLPEVAEVLIRIAMEEAQHAAHFAEMNGLISENLKENIEMMLKGECMANKEKKAAATKAKELGIDPAHDFFDESSRDEARHAKMLKGILDRYFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58157</accession>
    <name>HEMTB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bacteriohemerythrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0747</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Oxygen-binding protein. May be involved in a storage mechanism or for delivery to oxygen-requiring enzymes. The oxygen-binding site contains two iron atoms.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the hemerythrin family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98740" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98740.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64393">
        <property type="entry name" value="C64393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496610.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58157"/>
    <dbReference type="SMR" id="Q58157"/>
    <dbReference type="STRING" id="243232.MJ_0747"/>
    <dbReference type="EnsemblBacteria" id="AAB98740">
        <property type="protein sequence ID" value="AAB98740"/>
        <property type="gene ID" value="MJ_0747"/>
    </dbReference>
    <dbReference type="GeneID" id="1451624"/>
    <dbReference type="KEGG" id="mja:MJ_0747"/>
    <dbReference type="eggNOG" id="arCOG06577">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086902_2_2_2"/>
    <dbReference type="InParanoid" id="Q58157"/>
    <dbReference type="OMA" id="AKHFKHE"/>
    <dbReference type="PhylomeDB" id="Q58157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12107">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00556">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023504">
        <property type="entry name" value="Bacteriohemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016131">
        <property type="entry name" value="Haemerythrin_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035938">
        <property type="entry name" value="Hemerythrin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012827">
        <property type="entry name" value="Hemerythrin_metal-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47188">
        <property type="entry name" value="SSF47188"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02481">
        <property type="entry name" value="hemeryth_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00550">
        <property type="entry name" value="HEMERYTHRINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0561">Oxygen transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000191844" description="Bacteriohemerythrin">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="147" mass="17478" checksum="A7B85A8542C17670" modified="2002-05-27" version="2">MKKEIIKWSKDFETGIKAFDDEHKILVKTLNDIYNLLNEGKRDEAKELLKRRVVNYAAKHFKHEEEVMEKYGYPDLERHRKTHEIFVKTVIEKLLPKIEEGSENDFRSALSFLVGWLTMHIAKPDKKYGEWFKEKGIVIEDEAVKID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58172</accession>
    <name>Y762_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter MJ0762</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0762</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tellurite-resistance/dicarboxylate transporter (TDT) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64395">
        <property type="entry name" value="B64395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870267.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58172"/>
    <dbReference type="SMR" id="Q58172"/>
    <dbReference type="STRING" id="243232.MJ_0762"/>
    <dbReference type="TCDB" id="2.A.16.4.4">
        <property type="family name" value="the telurite-resistance/dicarboxylate transporter (tdt) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98753">
        <property type="protein sequence ID" value="AAB98753"/>
        <property type="gene ID" value="MJ_0762"/>
    </dbReference>
    <dbReference type="GeneID" id="1451639"/>
    <dbReference type="KEGG" id="mja:MJ_0762"/>
    <dbReference type="eggNOG" id="arCOG04355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030057_6_4_2"/>
    <dbReference type="InParanoid" id="Q58172"/>
    <dbReference type="OMA" id="APFYGCL"/>
    <dbReference type="OrthoDB" id="47545at2157"/>
    <dbReference type="PhylomeDB" id="Q58172"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000319">
        <property type="term" value="F:sulfite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000316">
        <property type="term" value="P:sulfite transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011552">
        <property type="entry name" value="TehA/Mae1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00816">
        <property type="entry name" value="tdt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000107020" description="Uncharacterized transporter MJ0762">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="276"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="304"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="342" mass="39535" checksum="08EFEC3E2C4955D8" modified="1996-11-01" version="1">MQSYIKNFESSWFAAVMGTGVLAVTSLFYSEYLPILKDISFLLFYFNILLFFVFLMLWILRWVKYPKNMIAELKHPVLSSFSPTVAVAMLVLGIDFILIKNNLFLGKIFWVFGAIGMFLFSLIVPFYMFKSESIKLDHVNPGWYIPPVGLIVIPIAGSLIMPHLTGVWHELTVLINYFGWGAGFFLYLALLAVVIYRFILHHPLPSAMAPTVWINLGPIGAGIVALINMVNNSPFITIKEPFYIFSFIFWGFGLWWSLMAIIMTLYYVKKLKLPYAMSWWAFIFPLGVYIASTHLVYKIFGFEIVDYIGFGLYWLLFFFWIVTLIKTINKVYSGELFKDKIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58199</accession>
    <name>Y789_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0789</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0789</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64398">
        <property type="entry name" value="E64398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870296.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58199"/>
    <dbReference type="STRING" id="243232.MJ_0789"/>
    <dbReference type="EnsemblBacteria" id="AAB98788">
        <property type="protein sequence ID" value="AAB98788"/>
        <property type="gene ID" value="MJ_0789"/>
    </dbReference>
    <dbReference type="GeneID" id="1451668"/>
    <dbReference type="KEGG" id="mja:MJ_0789"/>
    <dbReference type="eggNOG" id="arCOG05051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161126_0_0_2"/>
    <dbReference type="OMA" id="FNIQATS"/>
    <dbReference type="OrthoDB" id="123237at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019270">
        <property type="entry name" value="DUF2283"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10049">
        <property type="entry name" value="DUF2283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107040" description="Uncharacterized protein MJ0789">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <sequence length="116" mass="13203" checksum="E00F5FD7D6EEB771" modified="1996-11-01" version="1">MKVKIDYDYENDNLLVYKEGAKSKKTLDLDDILIDFDENGDVVGIEILNASKLFNVDKYDLLKNLIKFEAVGKITKDLITLNIKLYLLRRKKEIIKESVVKGLNTIGLKEGEVVIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58248</accession>
    <name>Y838_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0838</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0838</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0575.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64404">
        <property type="entry name" value="F64404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870352.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58248"/>
    <dbReference type="SMR" id="Q58248"/>
    <dbReference type="EnsemblBacteria" id="AAB98843">
        <property type="protein sequence ID" value="AAB98843"/>
        <property type="gene ID" value="MJ_0838"/>
    </dbReference>
    <dbReference type="GeneID" id="1451726"/>
    <dbReference type="KEGG" id="mja:MJ_0838"/>
    <dbReference type="eggNOG" id="arCOG02469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110109_0_0_2"/>
    <dbReference type="InParanoid" id="Q58248"/>
    <dbReference type="OMA" id="IQHYPCR"/>
    <dbReference type="OrthoDB" id="56837at2157"/>
    <dbReference type="PhylomeDB" id="Q58248"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107075" description="Uncharacterized protein MJ0838">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="223" mass="25837" checksum="1F592F76C84EC37F" modified="1996-11-01" version="1">MKVAILTDGVYGDRAYNTIKSKFPCDFITVKYYGDFDEITISENTIEKLKDYDLFITYTLNPDLTYELVRKIKELNNKAFVLVGAWKGEGFKKQIESFGNAFCPYLMCDIDEDELKDYKDYLDNYPHLKEFLKYFGKPKVKLYIKNNKIEKIDVLREAPCGSTSETLKEFVGREFNDKTLIDIGLRVQHFCRAGKIRLFVEKEGKKTKAGKILVSGIQVIQIP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58250</accession>
    <name>MFNF_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">(4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase</fullName>
            <ecNumber evidence="1">2.5.1.131</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">4-[[4-(2-aminoethyl)phenoxy]-methyl]-2-furanmethanamine-glutamate synthase</fullName>
            <shortName evidence="3">APMF-Glu synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">mfnF</name>
        <name type="ordered locus">MJ0840</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2015" name="J. Bacteriol." volume="197" first="2850" last="2858">
            <title>Identification of the final two genes functioning in methanofuran biosynthesis in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Wang Y."/>
                <person name="Xu H."/>
                <person name="Jones M.K."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="26100040"/>
            <dbReference type="DOI" id="10.1128/JB.00401-15"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation between 5-(aminomethyl)-3-furanmethanol diphosphate (F1-PP) and gamma-glutamyltyramine to produce APMF-Glu.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[5-(aminomethyl)furan-3-yl]methyl diphosphate + gamma-L-glutamyltyramine = (4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:47840"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83425"/>
            <dbReference type="ChEBI" id="CHEBI:88054"/>
            <dbReference type="ChEBI" id="CHEBI:88055"/>
            <dbReference type="EC" id="2.5.1.131"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MfnF family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.131" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64404">
        <property type="entry name" value="H64404"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58250"/>
    <dbReference type="SMR" id="Q58250"/>
    <dbReference type="STRING" id="243232.MJ_0840"/>
    <dbReference type="EnsemblBacteria" id="AAB98845">
        <property type="protein sequence ID" value="AAB98845"/>
        <property type="gene ID" value="MJ_0840"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0840"/>
    <dbReference type="eggNOG" id="arCOG04369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060932_0_0_2"/>
    <dbReference type="InParanoid" id="Q58250"/>
    <dbReference type="OMA" id="TDCFADR"/>
    <dbReference type="PhylomeDB" id="Q58250"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-19568"/>
    <dbReference type="BRENDA" id="2.5.1.131">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002756">
        <property type="entry name" value="MfnF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03123">
        <property type="entry name" value="one_C_unchar_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107076" description="(4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="26100040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="26100040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="26100040"/>
        </source>
    </evidence>
    <sequence length="330" mass="37377" checksum="5A3EAA6D2125D829" modified="1996-11-01" version="1">MKIMILGIDIGGANTKITEIEGDNYKIHHIYFPMWKKKDELEDLLKNYNDNVDYVALVMTAELADCYKTKKEGVEDIIDKVEKAFNCPVYVFDVNGNFLTSEEAKKNYLDVSASNWNATAKFVAEFIKDSCILVDMGSTTTDIIPIKDKEVLAEKTDLDRLMNNQLVYVGTLRTPVSFLANKIEFRGKLTNLSSEYFAITADISLILNKITEEDYTCDTPDGAGKDFESCLTRLVRVLCADREMVKDDELIDFANKLYNKLLELIRENVDTIAKRYNLNDVVITGLGEEILKDALDEYNIISIKETYGKDVSLATPSFAVAKLLQKQLDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58277</accession>
    <name>AMTAB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable threonylcarbamoyladenosine tRNA methylthiotransferase</fullName>
            <ecNumber>2.8.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>tRNA-t(6)A37 methylthiotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0867</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur carrier]-SH + AH2 + N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64408">
        <property type="entry name" value="C64408"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58277"/>
    <dbReference type="SMR" id="Q58277"/>
    <dbReference type="STRING" id="243232.MJ_0867"/>
    <dbReference type="EnsemblBacteria" id="AAB98872">
        <property type="protein sequence ID" value="AAB98872"/>
        <property type="gene ID" value="MJ_0867"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0867"/>
    <dbReference type="eggNOG" id="arCOG01358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018697_4_2_2"/>
    <dbReference type="InParanoid" id="Q58277"/>
    <dbReference type="OMA" id="CEHFHIP"/>
    <dbReference type="PhylomeDB" id="Q58277"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:N6-threonylcarbomyladenosine methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061712">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035600">
        <property type="term" value="P:tRNA methylthiolation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00089">
        <property type="entry name" value="TIGR00089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000141760" description="Probable threonylcarbamoyladenosine tRNA methylthiotransferase">
        <location>
            <begin position="1"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="MTTase N-terminal" evidence="3">
        <location>
            <begin position="12"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="4">
        <location>
            <begin position="141"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="2">
        <location>
            <begin position="373"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q5VV42"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="427" mass="49090" checksum="CF9834E3B849F0FF" modified="1996-11-01" version="1">MWLYYLQVVMDMRVYVEGYGCVLNTADTEIIKNSLKKHGFEVVNNLEEADIAIINTCVVRLETENRMIYRINELKNLGKEVVVAGCLPKALKNKVKGFLHIYPREAHKAGEILKNYVEKHYRMPYIEEDINKTLYKKLDYLKPSLITPLPICEGCIGNCSYCIVKIARGGLISYPREKIVNKAKELINKGAKCLLITAQDTACYGFDIGDNLANLLNELTQIKGEFIMRVGMMHAKNAELILDELIEVYQNEKVGKFLHLPLQSGDDEILKRMKRGYTVDEFKDIVNEFRRKIKNLCFTTDIIVGFPGETEEQFQNTLEVLRELKPDYIHGAKYSQRKGTEAAKMKQIDTKIRKRRSEILDKLRRELSYLNNKKYIGKAMKVLVLDEGKGYTDNFKVVKFEGGEVGEFRKVKITDAKTFGLKGELIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58355</accession>
    <name>Y945_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0945</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0945</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64418">
        <property type="entry name" value="A64418"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58355"/>
    <dbReference type="SMR" id="Q58355"/>
    <dbReference type="STRING" id="243232.MJ_0945"/>
    <dbReference type="EnsemblBacteria" id="AAB98955">
        <property type="protein sequence ID" value="AAB98955"/>
        <property type="gene ID" value="MJ_0945"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0945"/>
    <dbReference type="eggNOG" id="arCOG08285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313146_0_0_2"/>
    <dbReference type="InParanoid" id="Q58355"/>
    <dbReference type="OMA" id="GTDFYRY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010496">
        <property type="entry name" value="3-keto-disaccharide_hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06439">
        <property type="entry name" value="DUF1080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107116" description="Uncharacterized protein MJ0945">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="224" mass="25416" checksum="1E9FF7E32C025DA9" modified="1996-11-01" version="1">MLLRQYINVPRLGEIMNLKELTVILIIPIVYLGVCGCFEIVPKSFYDNFSSYNVGDKAPFGEWKVKEGGFKIEAILSEDKKTLNKVAVPINNGIIYIDKNYTDFKFIVDIKRLEESDSPKIYFRLINNANAGYYIDIEGFDRGYVLYKFNGTKVEKLAESYDAAPAGTDFYRYEVVAKDNKIIFLAGGQKYIEYTDNNTPILKGGIGIGGGRAYYDNVRVEPIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58364</accession>
    <name>Y954_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0954</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0954</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mj S-layer protein family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64419">
        <property type="entry name" value="B64419"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58364"/>
    <dbReference type="SMR" id="Q58364"/>
    <dbReference type="EnsemblBacteria" id="AAB98959">
        <property type="protein sequence ID" value="AAB98959"/>
        <property type="gene ID" value="MJ_0954"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0954"/>
    <dbReference type="eggNOG" id="arCOG03420">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_640322_0_0_2"/>
    <dbReference type="OMA" id="CVLALNQ"/>
    <dbReference type="PhylomeDB" id="Q58364"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000032622" description="Uncharacterized protein MJ0954">
        <location>
            <begin position="30"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="440" mass="50745" checksum="22988D6DD2534A84" modified="1996-11-01" version="1" precursor="true">MLRLQMMEGLIVKRTLLLILLLVISVSYALPIEPIIYVNKSTVDYQNAKILMDNFYSSREININGDNVTIVINDIMYIPSIDELEIKNGDKNLIIKFDRDGNKVKYKDIECIEYLNLKKGEEISLFNKSYIVEDITSNYVILKEKDGKEVLTNESFEYDGYKVVVKLVSSDLNTIIVDIYKNEKVLDSPKLTKGKIYYMKGGTLGLMYENCTRIGKGYRFTFRVYSTIKIEEGEDYPLDKEFKVKEISTDKIKLEYKNIDSLGNEIYLFNYTIIPEKCYKDYVLFKVIKRKEKTVDVKDVAYIGDGIYAVKVNNTVHVFYKGKELKNHEKIYLGSVDVYSSNPLNVNKDIILIGGPKVNKIVKELEDKGLLKVNISTNYPGNNRGIILKIKNPYNDNNIYILAGSDRWGTKAAILVFLTKYNDEDTLMVEWDKGEIKIIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58374</accession>
    <name>Y964_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0964</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0964</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HyuA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64420">
        <property type="entry name" value="D64420"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58374"/>
    <dbReference type="SMR" id="Q58374"/>
    <dbReference type="STRING" id="243232.MJ_0964"/>
    <dbReference type="EnsemblBacteria" id="AAB98966">
        <property type="protein sequence ID" value="AAB98966"/>
        <property type="gene ID" value="MJ_0964"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0964"/>
    <dbReference type="eggNOG" id="arCOG01511">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002157_1_2_2"/>
    <dbReference type="InParanoid" id="Q58374"/>
    <dbReference type="OMA" id="HAMTVSV"/>
    <dbReference type="PhylomeDB" id="Q58374"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017168">
        <property type="term" value="F:5-oxoprolinase (ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008040">
        <property type="entry name" value="Hydant_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05378">
        <property type="entry name" value="Hydant_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000208586" description="Uncharacterized protein MJ0964">
        <location>
            <begin position="1"/>
            <end position="680"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="680" mass="75843" checksum="D69AEA8C4F4555CD" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58391</accession>
    <name>T2M1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II restriction enzyme MjaI</fullName>
            <shortName>R.MjaI</shortName>
            <ecNumber>3.1.21.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Endonuclease MjaI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Type-2 restriction enzyme MjaI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaIR</name>
        <name type="ordered locus">MJ0984</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="patent" date="1999-03-11" number="WO9911821">
            <title>Method for screening restriction endonucleases.</title>
            <authorList>
                <person name="Noren C.J."/>
                <person name="Roberts R.J."/>
                <person name="Patti J."/>
                <person name="Byrd D.R."/>
                <person name="Morgan R.D."/>
            </authorList>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-CTAG-3'; the cleavage site is unknown.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.21.4"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.21.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64422">
        <property type="entry name" value="H64422"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58391"/>
    <dbReference type="REBASE" id="1221">
        <property type="entry name" value="MjaI"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98987">
        <property type="protein sequence ID" value="AAB98987"/>
        <property type="gene ID" value="MJ_0984"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0984"/>
    <dbReference type="eggNOG" id="arCOG11279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120987_0_0_2"/>
    <dbReference type="InParanoid" id="Q58391"/>
    <dbReference type="OMA" id="WEEYYET"/>
    <dbReference type="PRO" id="PR:Q58391"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019068">
        <property type="entry name" value="Restrct_endonuc_II_MjaI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09568">
        <property type="entry name" value="RE_MjaI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000077332" description="Type II restriction enzyme MjaI">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <sequence length="222" mass="25962" checksum="9A254A208AB8AC02" modified="1996-11-01" version="1">MVKLMKKLEDKKGIIEITFEEEREILELPSKPELPKYASQLINLANIFSQGTRPKVVGQMSELIKEFRKTGGRTFEDWKKWYLQKYPNAIDEATEKIWNMLNNFKETLEQLERDDVRKWVEDLVLIKTYEGLMLQDAILKKVAEELGGNYRPSTIEEESKGIDGVIIIDDKEIPVSIKSKTYVNQEKHLSEELKGHLIIYEKKKNKIIVDYSDLLDLVENTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58416</accession>
    <name>Y1010_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ1010</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1010</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64426">
        <property type="entry name" value="A64426"/>
    </dbReference>
    <dbReference type="PDB" id="3BJO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A=278-377"/>
    </dbReference>
    <dbReference type="PDBsum" id="3BJO"/>
    <dbReference type="AlphaFoldDB" id="Q58416"/>
    <dbReference type="SMR" id="Q58416"/>
    <dbReference type="STRING" id="243232.MJ_1010"/>
    <dbReference type="EnsemblBacteria" id="AAB99015">
        <property type="protein sequence ID" value="AAB99015"/>
        <property type="gene ID" value="MJ_1010"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1010"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q58416"/>
    <dbReference type="OMA" id="VWNAIKI"/>
    <dbReference type="PhylomeDB" id="Q58416"/>
    <dbReference type="EvolutionaryTrace" id="Q58416"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184673" description="Uncharacterized ATP-binding protein MJ1010">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="299"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="306"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="312"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="318"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="327"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="332"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="336"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="341"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="352"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="357"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="361"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="367"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3BJO"/>
        </source>
    </evidence>
    <sequence length="377" mass="44463" checksum="50AC996D843166A5" modified="1997-11-01" version="1">MINMKFFNRVEEIKEILSILEEEPNLIYFIYGPINSGKTALINEIINNRLDKNKYVVFYIDLREIFISKYDEFEAKLRATKTFGFRLIIEVLFEEYEDDKKPIEIIRSLIKDAPSLCGIPTPKNTLEEILKKKTTKNVFKYITNILMDIKREGKQPIIIIDELQKIGDMKINGFLIYELFNYFVSLTKHKHLCHVFCLSSDSLFIERVYNEAMLKERVDYILVDDFDKETALKFIDFLSEEILNKKLSDEDKELIYSYVGGKPILIINVIGKLKHKNLKDVLNILLMDEISKLKDFLSNLDYIKPKVNIEEEIIEIRKEDIINALKLFKGKYEIEVDKIPKAVYVYLVKKNILFLYPQRGTLKPQSFLVWNAIKRVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58417</accession>
    <name>Y1011_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1011</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1011</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64426">
        <property type="entry name" value="B64426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870524.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58417"/>
    <dbReference type="SMR" id="Q58417"/>
    <dbReference type="STRING" id="243232.MJ_1011"/>
    <dbReference type="DNASU" id="1451908"/>
    <dbReference type="EnsemblBacteria" id="AAB99012">
        <property type="protein sequence ID" value="AAB99012"/>
        <property type="gene ID" value="MJ_1011"/>
    </dbReference>
    <dbReference type="GeneID" id="1451908"/>
    <dbReference type="KEGG" id="mja:MJ_1011"/>
    <dbReference type="eggNOG" id="arCOG00232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1933262_0_0_2"/>
    <dbReference type="OrthoDB" id="135155at2157"/>
    <dbReference type="PhylomeDB" id="Q58417"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107141" description="Uncharacterized protein MJ1011">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <sequence length="130" mass="15278" checksum="377FCAAB4F027635" modified="1996-11-01" version="1">MPKKFDDIVNEMDRKIELLGEEIIKNLNLSVEGYCTNKKDICNLVIYKNNNIIKNLESLEMYSVKALCLYRPVSKDLRKLLTIIKLCSMLEKIEECAVKISFVLLNSKFNFDRNDKYIKRMASLTEEIIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58455</accession>
    <name>Y1055_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1055</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1055</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64431">
        <property type="entry name" value="F64431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870568.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58455"/>
    <dbReference type="SMR" id="Q58455"/>
    <dbReference type="STRING" id="243232.MJ_1055"/>
    <dbReference type="EnsemblBacteria" id="AAB99057">
        <property type="protein sequence ID" value="AAB99057"/>
        <property type="gene ID" value="MJ_1055"/>
    </dbReference>
    <dbReference type="GeneID" id="1451952"/>
    <dbReference type="KEGG" id="mja:MJ_1055"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="InParanoid" id="Q58455"/>
    <dbReference type="OMA" id="GVRTSWG"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="PhylomeDB" id="Q58455"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000183264" description="Uncharacterized protein MJ1055">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="326" mass="38369" checksum="4A45A975ECC0FB78" modified="1996-11-01" version="1">MKYKNILVTGSAGFIGFHLSKYLMDNYEDLKVIGIDNLNNYYNPVLKEKRNEILKNYENYTFIKLDFSDWDDLVENLKDKEIDLIVHLGAQAGVRYSLQNPWAYIKSNEMGTLNIFEFARRFDIEKVVYASSSSVYGGNRKIPFSEDDRVDKPISLYASTKRSNELMAHVYHHLYGIKMIGLRFFTVYGEYGRPDMAYFKFAKNILLGKEIEVYNYGNMERDFTYISDVVDGILRAIKKDFDYEIFNLGNSKPVKLMYFIELIEKYLNKKAKKKFLPMQDGDVLRTYADLSKSEKLLGYKPKVTIEEGLKRFCNWFLENKDWLLRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58462</accession>
    <name>Y1062_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1062</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1062</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64432">
        <property type="entry name" value="E64432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870575.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3EO4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.19 A"/>
        <property type="chains" value="A/B/C/D=324-484"/>
    </dbReference>
    <dbReference type="PDBsum" id="3EO4"/>
    <dbReference type="AlphaFoldDB" id="Q58462"/>
    <dbReference type="SMR" id="Q58462"/>
    <dbReference type="STRING" id="243232.MJ_1062"/>
    <dbReference type="DNASU" id="1451959"/>
    <dbReference type="EnsemblBacteria" id="AAB99065">
        <property type="protein sequence ID" value="AAB99065"/>
        <property type="gene ID" value="MJ_1062"/>
    </dbReference>
    <dbReference type="GeneID" id="1451959"/>
    <dbReference type="KEGG" id="mja:MJ_1062"/>
    <dbReference type="eggNOG" id="arCOG06581">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023406_0_1_2"/>
    <dbReference type="InParanoid" id="Q58462"/>
    <dbReference type="OrthoDB" id="6063at2157"/>
    <dbReference type="PhylomeDB" id="Q58462"/>
    <dbReference type="EvolutionaryTrace" id="Q58462"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020023">
        <property type="entry name" value="PseG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03590">
        <property type="entry name" value="PseG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107155" description="Uncharacterized protein MJ1062">
        <location>
            <begin position="1"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="334"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="324"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="333"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="341"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="344"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="354"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="383"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="394"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="408"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="412"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="427"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="446"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="456"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="468"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="477"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3EO4"/>
        </source>
    </evidence>
    <sequence length="484" mass="56694" checksum="E1764675A97CFCB2" modified="1996-11-01" version="1">MKIAIITDGSVEMGMGHVYRTLSLANELRKFNVNEIIFFTKSDEDVIKKIEENGFKVIKCSDNNDILKNIKNIKPDVVIIDDLGIEEDFAKNIRELCKKLIFFDNPNPSSNKYADIVVNAIVGSELKNRKYFDEENKTLYFYGPKYLILRNEFYKVKKEMLSRSKNKETKNILIAFGGSDPSNLTCKVLEELLSKDRDFNINVVLGPKFQYEDELNNLLKRYSKSDKIKIYKNIDNMAELMKDNDLIITSPGMTMFEALFLGIPVVVLYQNELQRECYDDYLKKISKTHLNPLKEGYFIDAEHTDLHIGKGKFEIIEAITNIYNCKKIGEDSKIIIRQITDNDLELLMAWRSNPLIYKFFYIQKEPLKWEEHYSWWMSRENRVDWIILLRENNTIRKVGSVNVSQLNTDNPEIGILIGEFFLWGKHIGRHSVSLVLKWLKNIGYKKAHARILENNIRSIKLFESLGFKKTKKGRENEWIYEVNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58472</accession>
    <name>Y1072_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1072</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1072</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the M.jannaschii MJ0023/MJ0349/MJ1072/MJ1074/MJ1107/MJECL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64433">
        <property type="entry name" value="G64433"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58472"/>
    <dbReference type="SMR" id="Q58472"/>
    <dbReference type="STRING" id="243232.MJ_1072"/>
    <dbReference type="EnsemblBacteria" id="AAB99079">
        <property type="protein sequence ID" value="AAB99079"/>
        <property type="gene ID" value="MJ_1072"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1072"/>
    <dbReference type="eggNOG" id="arCOG09652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165881_0_0_2"/>
    <dbReference type="OMA" id="EDIMLAE"/>
    <dbReference type="PhylomeDB" id="Q58472"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107158" description="Uncharacterized protein MJ1072">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="116" mass="13595" checksum="68BBDBEE2A56BC78" modified="1997-11-01" version="1">MIYFGGIMAIAYAKLYEIIAKYIKDEKRAEELYNAVVEVIKEEKIIVKHELKDELKNELATKEDIMLAEERILRYVDNRFNQLDKKMTVGFVILILLYILTNPNAIELIKLLFGVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58502</accession>
    <name>DUT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Deoxyuridine 5'-triphosphate nucleotidohydrolase</fullName>
            <shortName evidence="1">dUTPase</shortName>
            <ecNumber evidence="1 2 3">3.6.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>MjDUT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">dUTP pyrophosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dut</name>
        <name type="ordered locus">MJ1102</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="20667" last="20672">
            <title>A bifunctional dCTP deaminase-dUTP nucleotidohydrolase from the hyperthermophilic archaeon Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Bjoernberg O."/>
                <person name="Neuhard J."/>
                <person name="Nyman P.O."/>
            </authorList>
            <dbReference type="PubMed" id="12670946"/>
            <dbReference type="DOI" id="10.1074/jbc.M213010200"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-10</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="11100" last="11106">
            <title>The Methanococcus jannaschii dCTP deaminase is a bifunctional deaminase and diphosphatase.</title>
            <authorList>
                <person name="Li H."/>
                <person name="Xu H."/>
                <person name="Graham D.E."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="12538648"/>
            <dbReference type="DOI" id="10.1074/jbc.M212460200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3">This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>dUTP + H2O = diphosphate + dUMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10248"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61555"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.6.1.23"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">0.4 uM for dUTP</KM>
            <KM evidence="2">103 uM for dUTP</KM>
            <text evidence="2">kcat is 4.6 sec(-1).</text>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="1 2 3">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the dCTP deaminase family. Archaeal dUTPase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99105" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.23" evidence="1 2 3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99105.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64437">
        <property type="entry name" value="E64437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496724.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58502"/>
    <dbReference type="SMR" id="Q58502"/>
    <dbReference type="STRING" id="243232.MJ_1102"/>
    <dbReference type="EnsemblBacteria" id="AAB99105">
        <property type="protein sequence ID" value="AAB99105"/>
        <property type="gene ID" value="MJ_1102"/>
    </dbReference>
    <dbReference type="GeneID" id="1451999"/>
    <dbReference type="KEGG" id="mja:MJ_1102"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103451_2_0_2"/>
    <dbReference type="InParanoid" id="Q58502"/>
    <dbReference type="OMA" id="WDAGYEG"/>
    <dbReference type="OrthoDB" id="94658at2157"/>
    <dbReference type="PhylomeDB" id="Q58502"/>
    <dbReference type="SABIO-RK" id="Q58502"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00666"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004170">
        <property type="term" value="F:dUTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00635">
        <property type="entry name" value="dUTPase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023537">
        <property type="entry name" value="dUTPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000153638" description="Deoxyuridine 5'-triphosphate nucleotidohydrolase">
        <location>
            <begin position="2"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12538648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12670946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="156" mass="18070" checksum="AF6E28A4CF91FF3D" modified="2007-01-23" version="4">MIIGANTSKNFFDNLEEEQIQQCGIDLRVWKIFKIEGEGVIDFSNEKRKLPNYIEIFNSEKDEHIKLDRGVYIVKVADYIKIPENVAGFAYPRSSLLRMGATLYSAVHDPGYEGRPEYLMQVFNPITIYKYARIAQIVFVECRDVKGVYEGIYKGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58544</accession>
    <name>CBIG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable cobalt-precorrin-5A hydrolase</fullName>
            <ecNumber>3.7.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cbiG</name>
        <name type="ordered locus">MJ1144</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of the ring A acetate delta-lactone of cobalt-precorrin-5A resulting in the loss of the C-20 carbon and its attached methyl group in the form of acetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Co-precorrin-5A + H2O = acetaldehyde + Co-precorrin-5B + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26281"/>
            <dbReference type="ChEBI" id="CHEBI:15343"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:60062"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="EC" id="3.7.1.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 5/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CbiG family.</text>
    </comment>
    <dbReference type="EC" id="3.7.1.12"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64442">
        <property type="entry name" value="G64442"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58544"/>
    <dbReference type="SMR" id="Q58544"/>
    <dbReference type="STRING" id="243232.MJ_1144"/>
    <dbReference type="PRIDE" id="Q58544"/>
    <dbReference type="EnsemblBacteria" id="AAB99144">
        <property type="protein sequence ID" value="AAB99144"/>
        <property type="gene ID" value="MJ_1144"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1144"/>
    <dbReference type="eggNOG" id="arCOG00651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028397_0_0_2"/>
    <dbReference type="InParanoid" id="Q58544"/>
    <dbReference type="OMA" id="GIGCNRG"/>
    <dbReference type="PhylomeDB" id="Q58544"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00561"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043779">
        <property type="term" value="F:cobalt-precorrin-5A acetaldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="SSF159664"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="SSF159672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107187" description="Probable cobalt-precorrin-5A hydrolase">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="323" mass="37500" checksum="E6476F3F39DDBCE4" modified="1996-11-01" version="1">MMIKIVYITKRGKKIAEEIKDVLDYYHYDNKVEPIKDFKIERNEGGFIFIMATGIVLRKFLDEIKNDKFKDPFVIICNENKELIPILSNHLGGGNYFSKLIANNINGRVIFTTATDVNGKVGIDELSKMLFLETPKRKHILDINKKILEEDVSLTLPKYWKLRNLNGYKISYHDKYEVVVDDSIRLKPLKIAVGLGARKGIERYKVYWAVKKALFLRNIPVWRVDAFATIEDKKHERGILETVNKFKKPLIIFKREEINEIYEKIDLEKSEFVYKHLGVYGVSEPASILAVKKLTNKDFDSIKLILKKFKRNGVTVAIATENL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-26" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58559</accession>
    <name>RPA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Replication factor A</fullName>
            <shortName>RF-A</shortName>
            <shortName>RP-A</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Replication factor A protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Replication protein A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Single-stranded DNA-binding protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>mjaSSB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rpa</name>
        <name type="ordered locus">MJ1159</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Proc. Natl. Acad. Sci. U.S.A." volume="95" first="14634" last="14639">
            <title>Identification and characterization of a single-stranded DNA-binding protein from the archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Kelly T.J."/>
                <person name="Simancek P."/>
                <person name="Brush G.S."/>
            </authorList>
            <dbReference type="PubMed" id="9843941"/>
            <dbReference type="DOI" id="10.1073/pnas.95.25.14634"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>SUBUNIT</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="submission" date="2009-02" db="PDB data bank">
            <title>Crystal structure of a domain of a replication factor a protein, from Methanocaldococcus jannaschii. Northeast structural genomics target mjr118e.</title>
            <authorList>
                <consortium name="Northeast structural genomics consortium (NESG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 170-274</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Probably plays an essential for replication of the chromosome, DNA recombination and repair (By similarity). Binds approximately 20 nucleotides.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer. Binds to single-stranded DNA sequences.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64444">
        <property type="entry name" value="F64444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870672.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3DM3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B/C=170-274"/>
    </dbReference>
    <dbReference type="PDBsum" id="3DM3"/>
    <dbReference type="AlphaFoldDB" id="Q58559"/>
    <dbReference type="SMR" id="Q58559"/>
    <dbReference type="STRING" id="243232.MJ_1159"/>
    <dbReference type="EnsemblBacteria" id="AAB99162">
        <property type="protein sequence ID" value="AAB99162"/>
        <property type="gene ID" value="MJ_1159"/>
    </dbReference>
    <dbReference type="GeneID" id="1452057"/>
    <dbReference type="KEGG" id="mja:MJ_1159"/>
    <dbReference type="eggNOG" id="arCOG01510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_415991_0_0_2"/>
    <dbReference type="InParanoid" id="Q58559"/>
    <dbReference type="OMA" id="NAFSRKW"/>
    <dbReference type="OrthoDB" id="8528at2157"/>
    <dbReference type="PhylomeDB" id="Q58559"/>
    <dbReference type="EvolutionaryTrace" id="Q58559"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000097430" description="Replication factor A">
        <location>
            <begin position="1"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OB 1">
        <location>
            <begin position="78"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OB 2">
        <location>
            <begin position="186"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OB 3">
        <location>
            <begin position="291"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OB 4">
        <location>
            <begin position="423"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="2">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="221"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="229"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="9843941"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3DM3"/>
        </source>
    </evidence>
    <sequence length="645" mass="73842" checksum="3F0AD1B78065DD4F" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58583</accession>
    <name>Y1183_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1183</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1183</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH862.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64447">
        <property type="entry name" value="F64447"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58583"/>
    <dbReference type="SMR" id="Q58583"/>
    <dbReference type="STRING" id="243232.MJ_1183"/>
    <dbReference type="EnsemblBacteria" id="AAB99184">
        <property type="protein sequence ID" value="AAB99184"/>
        <property type="gene ID" value="MJ_1183"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1183"/>
    <dbReference type="eggNOG" id="arCOG04847">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682726_0_0_2"/>
    <dbReference type="InParanoid" id="Q58583"/>
    <dbReference type="OMA" id="AMPKIGV"/>
    <dbReference type="PhylomeDB" id="Q58583"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009183">
        <property type="entry name" value="UCP004962"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09897">
        <property type="entry name" value="DUF2124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004962">
        <property type="entry name" value="UCP004962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107206" description="Uncharacterized protein MJ1183">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="161" mass="18310" checksum="B1214A1F0AEC5678" modified="1996-11-01" version="1">MGDIMTLKLLKEENGLSPMLREFRTLVRDSNIEKVAFVGSVGVCQPFAELFGYAIRDKECYFIPDGDLNKVKKLVIKDIGMQMEDFENLNKVDAIVLFGGLAMPKYGVEVDKIKELINKLSPKKVIGVCFMSIFQKAGWDKEIDFDYLMDGFIKVSIYCKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58591</accession>
    <name>VHUG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>F420-non-reducing hydrogenase vhu subunit G</fullName>
            <ecNumber>1.12.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vhuG</name>
        <name type="ordered locus">MJ1191</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">The F420-non-reducing hydrogenase vhu is composed of four subunits; VhuA, VhuD, VhuG and VhuU.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.99.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64448">
        <property type="entry name" value="F64448"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58591"/>
    <dbReference type="SMR" id="Q58591"/>
    <dbReference type="STRING" id="243232.MJ_1191"/>
    <dbReference type="EnsemblBacteria" id="AAB99193">
        <property type="protein sequence ID" value="AAB99193"/>
        <property type="gene ID" value="MJ_1191"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1191"/>
    <dbReference type="eggNOG" id="arCOG02472">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053270_0_0_2"/>
    <dbReference type="InParanoid" id="Q58591"/>
    <dbReference type="OMA" id="GSWPEDI"/>
    <dbReference type="PhylomeDB" id="Q58591"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000204361" description="F420-non-reducing hydrogenase vhu subunit G">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="288" mass="31438" checksum="82F598BBD9B068B3" modified="1996-11-01" version="1">MITLAVKVGMIQLCGCSGCHISLLDLHDKLLEVLPNLEIVYAPIIADPKEIPEGIDVFLVEGGIRNEHDEHLIHEIREKSKIVIAWGTCAAYGGIPGLGNLYKKEELLNYVYSTDSTENKGEIPSEEIPPLEEYVKPIKDFIKVDYTIPGCPPTPKMIADAIIALLNGEEPKLPTKIVCDECPRKKENVFPETFKRTHEGRPDPERCLFEQGYTCLGFATRAGCGAKCPSAGVPCRGCFGKTDKSLDLGANAANVLANAGEAALEIPDKVALLNRFTLPDALINRKAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58609</accession>
    <name>PSS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber>2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pssA</name>
        <name type="ordered locus">MJ1212</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cell membrane</location>
            <topology>Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64451">
        <property type="entry name" value="C64451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870724.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="7B1K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-201"/>
    </dbReference>
    <dbReference type="PDB" id="7B1L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B=1-201"/>
    </dbReference>
    <dbReference type="PDB" id="7B1N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-201"/>
    </dbReference>
    <dbReference type="PDB" id="7POW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.51 A"/>
        <property type="chains" value="A/B=1-201"/>
    </dbReference>
    <dbReference type="PDBsum" id="7B1K"/>
    <dbReference type="PDBsum" id="7B1L"/>
    <dbReference type="PDBsum" id="7B1N"/>
    <dbReference type="PDBsum" id="7POW"/>
    <dbReference type="AlphaFoldDB" id="Q58609"/>
    <dbReference type="SMR" id="Q58609"/>
    <dbReference type="STRING" id="243232.MJ_1212"/>
    <dbReference type="DNASU" id="1452108"/>
    <dbReference type="EnsemblBacteria" id="AAB99214">
        <property type="protein sequence ID" value="AAB99214"/>
        <property type="gene ID" value="MJ_1212"/>
    </dbReference>
    <dbReference type="GeneID" id="1452108"/>
    <dbReference type="KEGG" id="mja:MJ_1212"/>
    <dbReference type="eggNOG" id="arCOG00671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_3_1_2"/>
    <dbReference type="InParanoid" id="Q58609"/>
    <dbReference type="OMA" id="YSICGML"/>
    <dbReference type="OrthoDB" id="68873at2157"/>
    <dbReference type="PhylomeDB" id="Q58609"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000056799" description="CDP-diacylglycerol--serine O-phosphatidyltransferase">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="3"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="10"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="111"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="7B1L"/>
        </source>
    </evidence>
    <sequence length="201" mass="22014" checksum="D45D3201941615CA" modified="1996-11-01" version="1">MFSIRKIITISDYVTMLNIITGLLAILLNSFSLIYLSIIFDSLDGYVARKTGTVSDFGAELDSISDVVSFGVAPAYLLYNNFESNLALISAIIFCLCGALRLARFGILNVKGFIGLPIPAGALLLVGFCQLINSYLINSILAILIGLLMISDIKYPKYPNKIFIYIFAVSLCLAIVGIPHFALMLCLIYAIYGIIKYIRGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q58628</accession>
    <name>PYCB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Pyruvate carboxylase subunit B</fullName>
            <ecNumber>6.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Pyruvic carboxylase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pycB</name>
        <name type="ordered locus">MJ1231</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Arch. Microbiol." volume="174" first="406" last="414">
            <title>A stable archaeal pyruvate carboxylase from the hyperthermophile Methanococcus jannaschii.</title>
            <authorList>
                <person name="Mukhopadhyay B."/>
                <person name="Patel V.J."/>
                <person name="Wolfe R.S."/>
            </authorList>
            <dbReference type="PubMed" id="11195096"/>
            <dbReference type="DOI" id="10.1007/s002030000225"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 190-195; 260-270; 277-289; 277-289; 309-325; 328-358; 370-380; 386-409; 422-438; 491-506 AND 491-506</scope>
        <scope>FUNCTION</scope>
        <scope>MASS SPECTROMETRY</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + hydrogencarbonate + pyruvate = ADP + H(+) + oxaloacetate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text>Inhibited by magnesium, when its concentration exceeded the ATP one, and by high concentration of ATP and alpha-ketoglutarate.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 8.5.</text>
        </phDependence>
        <temperatureDependence>
            <text>Optimum temperature is 80-90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text>Heterooctamer of four A and four B subunits.</text>
    </comment>
    <comment type="mass spectrometry" mass="64160.0" method="MALDI" evidence="4"/>
    <dbReference type="EC" id="6.4.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64453">
        <property type="entry name" value="F64453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870743.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58628"/>
    <dbReference type="SMR" id="Q58628"/>
    <dbReference type="STRING" id="243232.MJ_1231"/>
    <dbReference type="PRIDE" id="Q58628"/>
    <dbReference type="EnsemblBacteria" id="AAB99233">
        <property type="protein sequence ID" value="AAB99233"/>
        <property type="gene ID" value="MJ_1231"/>
    </dbReference>
    <dbReference type="GeneID" id="1452127"/>
    <dbReference type="KEGG" id="mja:MJ_1231"/>
    <dbReference type="eggNOG" id="arCOG02095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_4_2_2"/>
    <dbReference type="InParanoid" id="Q58628"/>
    <dbReference type="OMA" id="GYHALEM"/>
    <dbReference type="OrthoDB" id="19729at2157"/>
    <dbReference type="PhylomeDB" id="Q58628"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005776">
        <property type="entry name" value="OadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="SSF51230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01108">
        <property type="entry name" value="oadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0092">Biotin</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146830" description="Pyruvate carboxylase subunit B">
        <location>
            <begin position="1"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="3">
        <location>
            <begin position="2"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="Biotinyl-binding" evidence="2">
        <location>
            <begin position="492"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-biotinyllysine" evidence="1 2">
        <location>
            <position position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="11195096"/>
        </source>
    </evidence>
    <sequence length="567" mass="63908" checksum="5E07800622545628" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q58645</accession>
    <name>Y1248_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1248</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1248</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64455">
        <property type="entry name" value="G64455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870760.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58645"/>
    <dbReference type="SMR" id="Q58645"/>
    <dbReference type="STRING" id="243232.MJ_1248"/>
    <dbReference type="EnsemblBacteria" id="AAB99258">
        <property type="protein sequence ID" value="AAB99258"/>
        <property type="gene ID" value="MJ_1248"/>
    </dbReference>
    <dbReference type="GeneID" id="1452145"/>
    <dbReference type="KEGG" id="mja:MJ_1248"/>
    <dbReference type="eggNOG" id="arCOG09678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727275_0_0_2"/>
    <dbReference type="OMA" id="RAQYWIM"/>
    <dbReference type="OrthoDB" id="97745at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107239" description="Uncharacterized protein MJ1248">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <sequence length="154" mass="18006" checksum="219316E7F5A7E585" modified="1996-11-01" version="1">MKMEVIEKLSELSGIDKKSLRRILIILEFSLRKKDGSPTSFAEKFNIKSFGDLYNYIRDVKSNLKRDHEIEGFNGLTEMWKSVAPRAQYWIMDTFGEENPRDALFSASVFTMRTFGIMLDNLLLLKKIIKTLDEYQKEVTEYVSAQKFEAEDLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58669</accession>
    <name>BPSA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(4)-bis(aminopropyl)spermidine synthase</fullName>
            <ecNumber evidence="1">2.5.1.128</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Branched-chain polyamine synthase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bpsA</name>
        <name type="ordered locus">MJ1273</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain polyamines, which support the growth of thermophiles under high-temperature conditions. Catalyzes the sequential condensation of spermidine with the aminopropyl groups of decarboxylated S-adenosylmethionines to produce N(4)-bis(aminopropyl)spermidine via N(4)-aminopropylspermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = 2 H(+) + N(4)-bis(aminopropyl)spermidine + 2 S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:44132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82771"/>
            <dbReference type="EC" id="2.5.1.128"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the branched-chain polyamine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.128" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64458">
        <property type="entry name" value="H64458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870786.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58669"/>
    <dbReference type="SMR" id="Q58669"/>
    <dbReference type="STRING" id="243232.MJ_1273"/>
    <dbReference type="EnsemblBacteria" id="AAB99279">
        <property type="protein sequence ID" value="AAB99279"/>
        <property type="gene ID" value="MJ_1273"/>
    </dbReference>
    <dbReference type="GeneID" id="1452171"/>
    <dbReference type="KEGG" id="mja:MJ_1273"/>
    <dbReference type="eggNOG" id="arCOG00913">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042160_0_0_2"/>
    <dbReference type="InParanoid" id="Q58669"/>
    <dbReference type="OMA" id="KSYMFRI"/>
    <dbReference type="OrthoDB" id="23573at2157"/>
    <dbReference type="PhylomeDB" id="Q58669"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01947">
        <property type="entry name" value="Aminopropyltransf_BpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014435">
        <property type="entry name" value="BpsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002723">
        <property type="entry name" value="BpsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01861">
        <property type="entry name" value="DUF43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005895">
        <property type="entry name" value="UCP005895_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107245" description="N(4)-bis(aminopropyl)spermidine synthase">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01947"/>
        </source>
    </evidence>
    <sequence length="350" mass="40420" checksum="2F301BF8976C22BB" modified="1996-11-01" version="1">MERILEKVRAKSEIPVYDKSIENVLSAILTTNDFWKIVDLSEEPLPLVADIIRILEEEGLVKISNGIEFTEKGNEFIKSYGIGAKDNSVCECCEGRGVSLKNYQDLLERFKEIVKNRPMPKHEYDQGFVTPECTISRIALMNSRGDLFNKDVLVLGDDDLTSIALMLSNLPKKIVVVDIDDRLINFIKEVAEQLNYKNIEVITLDLRKPLPEKYSRAFDTFITDPPETVYAVKTFIGRGISALKGERRAGYFGITRRESSLDKWREIQRTLINDFNVVITDIIRNFNHYVNWGYEEETRAWKLAPVKKKPEDIWYKSYMFRIETLKDSRGFEEEVDVGDELYNDAESSTT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-18" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q58687</accession>
    <name>Y1291_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1291</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1291</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64461">
        <property type="entry name" value="B64461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870807.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58687"/>
    <dbReference type="STRING" id="243232.MJ_1291"/>
    <dbReference type="EnsemblBacteria" id="AAB99297">
        <property type="protein sequence ID" value="AAB99297"/>
        <property type="gene ID" value="MJ_1291"/>
    </dbReference>
    <dbReference type="GeneID" id="1452192"/>
    <dbReference type="KEGG" id="mja:MJ_1291"/>
    <dbReference type="eggNOG" id="arCOG05987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154366_0_0_2"/>
    <dbReference type="OMA" id="WASVNID"/>
    <dbReference type="OrthoDB" id="67638at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014012" description="Uncharacterized protein MJ1291">
        <location>
            <begin position="31"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="240" mass="27112" checksum="CB527B46199362DC" modified="1998-06-01" version="2" precursor="true">MNKSGMSLIITMLLLIGTAIVIGAAYYAWSNKVFSDTTEKITPTIKSSIGNIIKPIEISTIETYYFTNLDLNGDSRITNNPEERFIQTIKLEFINNIDEDLNVNTRIYCLTPNVSWASVNIDDSSNNLLLDRDENPYNYSGQYVYFNGTVYYSSMKFYDENGKLFYAAASNGNALNTSNLLDLIDLNCPTESFLLKGNSKTDINYYILINNTKVPNTIIFEIIASTKYGDVEKKITFEIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q58733</accession>
    <name>Y1337_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1337</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1337</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64466">
        <property type="entry name" value="H64466"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870855.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58733"/>
    <dbReference type="STRING" id="243232.MJ_1337"/>
    <dbReference type="EnsemblBacteria" id="AAB99358">
        <property type="protein sequence ID" value="AAB99358"/>
        <property type="gene ID" value="MJ_1337"/>
    </dbReference>
    <dbReference type="GeneID" id="1452240"/>
    <dbReference type="KEGG" id="mja:MJ_1337"/>
    <dbReference type="eggNOG" id="arCOG05078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_979811_0_0_2"/>
    <dbReference type="InParanoid" id="Q58733"/>
    <dbReference type="OMA" id="HYIKPKI"/>
    <dbReference type="OrthoDB" id="45436at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107281" description="Uncharacterized protein MJ1337">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <sequence length="231" mass="25675" checksum="203926DBCD472FAE" modified="1996-11-01" version="1">MIGAVLKTLEYFDEKDVKVITTGDIGEGDGSLKIYDALKEIDDDLVIIHYIKPKISKIREINFSPKIIADAGGMYAAKAANIGDKFYLFLPDVGELAFLADEKASHPAYVRGFISEIDDNEVPKLIERDYKLKMPKYMVVKGETDYTIREEKIIDKIKEPKIKAMECIGGTGDTLTEIVSSLISVDFKTEEALSLGCKINRKLGEIANVNPNTQITEIINAIPKALENTLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-04" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58745</accession>
    <name>Y1350_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1350</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1350</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FwdC/FmdC family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64468">
        <property type="entry name" value="E64468"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58745"/>
    <dbReference type="SMR" id="Q58745"/>
    <dbReference type="STRING" id="243232.MJ_1350"/>
    <dbReference type="EnsemblBacteria" id="AAB99361">
        <property type="protein sequence ID" value="AAB99361"/>
        <property type="gene ID" value="MJ_1350"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1350"/>
    <dbReference type="eggNOG" id="arCOG00096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078510_1_0_2"/>
    <dbReference type="InParanoid" id="Q58745"/>
    <dbReference type="OMA" id="PYIEEFC"/>
    <dbReference type="PhylomeDB" id="Q58745"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00981">
        <property type="entry name" value="arch_gltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035710">
        <property type="entry name" value="Archaeal_gltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012061">
        <property type="entry name" value="Glu_synth_lsu_3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006519">
        <property type="entry name" value="GOGAT_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000144200" description="Uncharacterized protein MJ1350">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="261" mass="28957" checksum="CB4023B5B88B0B58" modified="1996-11-01" version="1">MKGMEEVVIDAKDMHYRELNEKIHEILRENPDIKKIVLKNVLGQRFIADGIQKKDLTIEIYGIPGGDLGMFMSGPTIIVHGNAEFAPGNTMDDGTIVIYGSSGDVTAHSMRGGKVFVRGDVGYRSGIHMKAYKDKVPVLVIGGRAKDFLGEYMAGGIIIVLNIDEKGNDLGKVKGRMIGTGIHGGAIYIRGEIDKDQLGVAADIKEFTEEDLEKIKPYIEEFCKWFNLPEDVKNKLLNSKWTKIAPISKRPFGKLYTPDLM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58755</accession>
    <name>Y1360_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1360</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1360</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64469">
        <property type="entry name" value="G64469"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58755"/>
    <dbReference type="STRING" id="243232.MJ_1360"/>
    <dbReference type="EnsemblBacteria" id="AAB99378">
        <property type="protein sequence ID" value="AAB99378"/>
        <property type="gene ID" value="MJ_1360"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1360"/>
    <dbReference type="eggNOG" id="arCOG05080">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392941_0_0_2"/>
    <dbReference type="OMA" id="DKTLIFM"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107297" description="Uncharacterized protein MJ1360">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <sequence length="99" mass="11595" checksum="1BF321FD8A283B3E" modified="1996-11-01" version="1">MWGSMPRRKIDKLYVKIYFEGNAIEGEYDFDAVTHLKNGILKYLWTGKKDPIIIWNMDNKSFTIIDPSKICAVEVQGSLMFLDDIPEKKLEMKSFSERD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q58761</accession>
    <name>KPRS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="1 3">RPPK</shortName>
            <ecNumber evidence="1 2">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-phospho-D-ribosyl alpha-1-diphosphate</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1 3">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1 3">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="1">P-Rib-PP synthase</shortName>
            <shortName evidence="1 3">PRPP synthase</shortName>
            <shortName evidence="1">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 3" type="primary">prs</name>
        <name type="ordered locus">MJ1366</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="Microbiol. Mol. Biol. Rev." volume="81" first="E00040" last="E00040">
            <title>Phosphoribosyl diphosphate (PRPP): biosynthesis, enzymology, utilization, and metabolic significance.</title>
            <authorList>
                <person name="Hove-Jensen B."/>
                <person name="Andersen K.R."/>
                <person name="Kilstrup M."/>
                <person name="Martinussen J."/>
                <person name="Switzer R.L."/>
                <person name="Willemoes M."/>
            </authorList>
            <dbReference type="PubMed" id="28031352"/>
            <dbReference type="DOI" id="10.1128/MMBR.00040-16"/>
        </citation>
        <scope>REVIEW</scope>
        <scope>COFACTOR</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="354" first="815" last="828">
            <title>Novel class III phosphoribosyl diphosphate synthase: structure and properties of the tetrameric, phosphate-activated, non-allosterically inhibited enzyme from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Kadziola A."/>
                <person name="Jepsen C.H."/>
                <person name="Johansson E."/>
                <person name="McGuire J."/>
                <person name="Larsen S."/>
                <person name="Hove-Jensen B."/>
            </authorList>
            <dbReference type="PubMed" id="16288921"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.10.001"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) IN COMPLEX WITH SUBSTRATES</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>PATHWAY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P). It can also use dATP as diphosphoryl donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>ATP + D-ribose 5-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1 2 4">Binds 2 Mg(2+) ions per subunit (PubMed:28031352). Mn(2+) is also accepted, but the activity is less than 15% of that obtained with Mg(2+), when assayed at pH 9.5 (PubMed:16288921).</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Activated by inorganic phosphate, with a maximal activity at 190 mM. Above this concentration inorganic phosphate progressively inhibits the kinase. Completely inhibited by ADP, and partially inhibited by alpha,beta-methylene ATP (mATP). Lack of allosteric regulation.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">2.6 mM for ATP (at pH 9 and 85 degrees Celsius)</KM>
            <KM evidence="2">2.8 mM for ribose 5-phosphate (at pH 9 and 85 degrees Celsius)</KM>
            <Vmax evidence="2">2.2 mmol/min/mg enzyme (at pH 9 and 85 degrees Celsius)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 9.5. The activity declines strongly above pH 10.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 85 degrees Celsius. 70% of maximal activity at 72 and 95 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="6">M.jannaschii PRPP synthase is more compact than the PRPP synthase in the B. subtilis subunit. This is mainly due to truncations of eight residues at the N terminus, 14 residues at the C terminus and to a seven-residue shorter loop.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 6">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99374" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99374.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64470">
        <property type="entry name" value="E64470"/>
    </dbReference>
    <dbReference type="PDB" id="1U9Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.65 A"/>
        <property type="chains" value="A/B/C/D=1-284"/>
    </dbReference>
    <dbReference type="PDB" id="1U9Z">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D=1-284"/>
    </dbReference>
    <dbReference type="PDBsum" id="1U9Y"/>
    <dbReference type="PDBsum" id="1U9Z"/>
    <dbReference type="AlphaFoldDB" id="Q58761"/>
    <dbReference type="SMR" id="Q58761"/>
    <dbReference type="STRING" id="243232.MJ_1366"/>
    <dbReference type="EnsemblBacteria" id="AAB99374">
        <property type="protein sequence ID" value="AAB99374"/>
        <property type="gene ID" value="MJ_1366"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1366"/>
    <dbReference type="eggNOG" id="arCOG00067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033546_2_2_2"/>
    <dbReference type="InParanoid" id="Q58761"/>
    <dbReference type="OMA" id="FGWARQD"/>
    <dbReference type="PhylomeDB" id="Q58761"/>
    <dbReference type="BRENDA" id="2.7.6.1">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58761"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009156">
        <property type="term" value="P:ribonucleoside monophosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_A">
        <property type="entry name" value="RibP_PPkinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037514">
        <property type="entry name" value="Rib-P_diPkinase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="PTHR10210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141238" description="Ribose-phosphate pyrophosphokinase">
        <location>
            <begin position="1"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2 7">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6 7">
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            <begin position="92"/>
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        <ligand>
            <name>ATP</name>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2 7">
        <location>
            <position position="212"/>
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        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2 7">
        <location>
            <begin position="216"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="2"/>
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        </location>
    </feature>
    <feature type="helix" evidence="9">
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            <begin position="7"/>
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    </feature>
    <feature type="helix" evidence="8">
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        </location>
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    <feature type="helix" evidence="8">
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            <begin position="167"/>
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    <feature type="strand" evidence="8">
        <location>
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    </feature>
    <feature type="strand" evidence="8">
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        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="216"/>
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        </location>
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        <location>
            <begin position="219"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="235"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="249"/>
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        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="259"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="272"/>
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        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="277"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16288921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="16288921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="28031352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="16288921"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="1U9Z"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="1U9Y"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="1U9Z"/>
        </source>
    </evidence>
    <sequence length="284" mass="31395" checksum="9EAE9805785B1127" modified="2002-10-25" version="2">MIVVSGSQSQNLAFKVAKLLNTKLTRVEYKRFPDNEIYVRIVDEINDDEAVIINTQKNQNDAIVETILLCDALRDEGVKKITLVAPYLAYARQDKKFNPGEAISIRALAKIYSNIVDKLITINPHETHIKDFFTIPFIYGDAVPKLAEYVKDKLNDPIVLAPDKGALEFAKTASKILNAEYDYLEKTRLSPTEIQIAPKTLDAKDRDVFIVDDIISTGGTMATAVKLLKEQGAKKIIAACVHPVLIGDALNKLYSAGVEEVVGTDTYLSEVSKVSVAEVIVDLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58770</accession>
    <name>Y1375_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized membrane protein MJ1375</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1375</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64471">
        <property type="entry name" value="F64471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870892.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58770"/>
    <dbReference type="SMR" id="Q58770"/>
    <dbReference type="STRING" id="243232.MJ_1375"/>
    <dbReference type="EnsemblBacteria" id="AAB99382">
        <property type="protein sequence ID" value="AAB99382"/>
        <property type="gene ID" value="MJ_1375"/>
    </dbReference>
    <dbReference type="GeneID" id="1452278"/>
    <dbReference type="KEGG" id="mja:MJ_1375"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_648322_0_0_2"/>
    <dbReference type="InParanoid" id="Q58770"/>
    <dbReference type="OMA" id="FIIIDSG"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="PhylomeDB" id="Q58770"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000166461" description="Uncharacterized membrane protein MJ1375">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="415" mass="45989" checksum="EC0EC85C65BA7865" modified="1998-06-01" version="2">MSLCKDSIYILMSNLYSKGMAYLFYFITAFLLGTEAFGILKGLMPIADTLTIFFSSGIPPAIAKFLAEEKEVDINKYIPILYLMILLSVVGFILTPYIKYILGGHYLNLPNILYFAVGLCVVASTVIAFSRGILQGLLKMKYLSLTWIVEYTAKVILVFILTLYLGIFGSLLSISLAYLVGGIFGLYLIYKALKGKFDFKKLIDIKNTTKNIFSNFNLDILRYSIPIALTSSSYRLFGDIDNIVIMSIMGGFWSGIYGYSSLISRGIFMFASAVSIPLLPRISKTKDLSLLKEGIIQNTIFSSIFVIGCLFFPEIPLIAFFKTANPEGILCLRILAISSLFMSYYTLISSALQGLGYAKISFYIILFGLVLNIILNLILVNAYGIVGGSLATLITSISVFLIGVFAILRIKKHII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="143" xmlns="http://uniprot.org/uniprot">
    <accession>Q58776</accession>
    <name>CARB2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Carbamoyl-phosphate synthase large chain, C-terminal section</fullName>
            <ecNumber>6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">carB2</name>
        <name type="ordered locus">MJ1381</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="domain">
        <text>Corresponds to the C-terminal section.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">CarB is split into two genes in M.jannaschii (MJ1378 and MJ1381).</text>
    </comment>
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    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="AlphaFoldDB" id="Q58776"/>
    <dbReference type="SMR" id="Q58776"/>
    <dbReference type="STRING" id="243232.MJ_1381"/>
    <dbReference type="EnsemblBacteria" id="AAB99391">
        <property type="protein sequence ID" value="AAB99391"/>
        <property type="gene ID" value="MJ_1381"/>
    </dbReference>
    <dbReference type="GeneID" id="1452284"/>
    <dbReference type="KEGG" id="mja:MJ_1381"/>
    <dbReference type="eggNOG" id="arCOG01594">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_3_4_2"/>
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    <dbReference type="OMA" id="CVQAVFA"/>
    <dbReference type="OrthoDB" id="4638at2157"/>
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    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
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    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0005737">
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
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    </dbReference>
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        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="CDD" id="cd01424">
        <property type="entry name" value="MGS_CPS_II"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-grasp"/>
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        <property type="entry name" value="ATP_grasp_subdomain_1"/>
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        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
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        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
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        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033937">
        <property type="entry name" value="MGS_CPS_CarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145076" description="Carbamoyl-phosphate synthase large chain, C-terminal section">
        <location>
            <begin position="1"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="208"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="MGS-like" evidence="3">
        <location>
            <begin position="476"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carbamoyl phosphate synthetic domain">
        <location>
            <begin position="81"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Allosteric domain">
        <location>
            <begin position="478"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="234"/>
            <end position="291"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="372"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="618" mass="68453" checksum="8C3D4D9C192A954E" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58777</accession>
    <name>Y1382_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0310 protein MJ1382</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1382</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0310 family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB99392"/>
        <property type="gene ID" value="MJ_1382"/>
    </dbReference>
    <dbReference type="GeneID" id="1452285"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="HAMAP" id="MF_00771">
        <property type="entry name" value="UPF0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022996">
        <property type="entry name" value="UPF0310"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000059635" description="UPF0310 protein MJ1382">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="146" mass="17299" checksum="DB51C30DE77A96D2" modified="1997-11-01" version="1">MAYWLCITNEDNWKVIKDKKIWGVAERHKNTINKVKVGDKLIIYEIQRSGKDYKPPYIRGVYEVVSEVYKDSSKIFKPTPRNPNEKFPYRVKLKEVKVFVPPINFKDLIPKLKFITNKKKWSGHLMGKAMREIPEEDYKLIIEAKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58792</accession>
    <name>Y1397_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1397</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1397</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAB99410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D64474"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58792"/>
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    <dbReference type="EnsemblBacteria" id="AAB99410">
        <property type="protein sequence ID" value="AAB99410"/>
        <property type="gene ID" value="MJ_1397"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1397"/>
    <dbReference type="eggNOG" id="arCOG07531">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_521413_0_0_2"/>
    <dbReference type="OMA" id="IPPIFRR"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00189">
        <property type="entry name" value="LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014014" description="Uncharacterized protein MJ1397">
        <location>
            <begin position="26"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="306"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="486" mass="55817" checksum="83BC9302A3D1240B" modified="1996-11-01" version="1" precursor="true">MGTMRSVYLIIIIILFFAFISLSFGEIYEYRGVVFYPNGSQTVDVSKLHIDYENPDAFSIYISYRNSSFYLPYSKNITLNLPPKKFNISITISASRISRDEWRVYYKIINNYPYSILFNISFPGGFNIKNASVLVPAKSYKIITLSKIQNSNILYFGDSNISFEVPAKLMIRYSLPIPFSIIKSNKILSNGSIEWTAIYIIKNDKNVSLNVNASYWAVVNNTKIDFGNYSYIIEPNENVSQSFNITSDYVPIFYLKFYAWRDVYETIKIKPAIKVDNSYIIGIGKVEGLSFNIPYYNYMEREIKKKKEEKENEESSKTINQMQRHKKEEKSQTQETKKPSKNEMNKQEKERKYPLIIEEKFKKVAAAIATVTTTSITAMLIPPIFRRRSYIVDKGIFSIKDLELLSGTVYVPEGCKLGNILPGGITIIKLTDVEKDLARDLHEIYDIPLNSAKAIILGVKYGGRVFLSDKKAYDVAVEIGLEAYLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58801</accession>
    <name>PYRI_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">pyrI</name>
        <name type="ordered locus">MJ1406</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Biol. Chem." volume="275" first="15820" last="15827">
            <title>Characterization of the aspartate transcarbamoylase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Hack E.S."/>
                <person name="Vorobyova T."/>
                <person name="Sakash J.B."/>
                <person name="West J.M."/>
                <person name="Macol C.P."/>
                <person name="Herve G."/>
                <person name="Williams M.K."/>
                <person name="Kantrowitz E.R."/>
            </authorList>
            <dbReference type="PubMed" id="10748118"/>
            <dbReference type="DOI" id="10.1074/jbc.M909220199"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text>Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PyrI family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64475">
        <property type="entry name" value="E64475"/>
    </dbReference>
    <dbReference type="PDB" id="2YWW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=1-149"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YWW"/>
    <dbReference type="AlphaFoldDB" id="Q58801"/>
    <dbReference type="SMR" id="Q58801"/>
    <dbReference type="STRING" id="243232.MJ_1406"/>
    <dbReference type="EnsemblBacteria" id="AAB99414">
        <property type="protein sequence ID" value="AAB99414"/>
        <property type="gene ID" value="MJ_1406"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1406"/>
    <dbReference type="eggNOG" id="arCOG04229">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128576_0_0_2"/>
    <dbReference type="InParanoid" id="Q58801"/>
    <dbReference type="OMA" id="CPNRNCI"/>
    <dbReference type="PhylomeDB" id="Q58801"/>
    <dbReference type="BRENDA" id="2.1.3.2">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58801"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="PTHR35805"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="SSF54893"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="SSF57825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000142331" description="Aspartate carbamoyltransferase regulatory chain">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="26"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="41"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="53"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="81"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="89"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="100"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="114"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="123"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2YWW"/>
        </source>
    </evidence>
    <sequence length="149" mass="16907" checksum="192B446466DF0ECE" modified="1996-11-01" version="1">MIPMEELKVKKITNGTVIDHIDAGKALMVFKVLNVPKETSVMIAINVPSKKKGKKDILKIEGIELKKEDVDKISLISPDVTINIIRNGKVVEKLKPQIPDEIEGTLKCTNPNCITNKEKVRGKFKIESKNPLKIRCYYCEKFLNEVIFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q58819</accession>
    <name>ENDA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>tRNA-splicing endonuclease</fullName>
            <ecNumber>4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">endA</name>
        <name type="ordered locus">MJ1424</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="EMBO J." volume="16" first="6290" last="6300">
            <title>RNA-protein interactions of an archaeal homotetrameric splicing endoribonuclease with an exceptional evolutionary history.</title>
            <authorList>
                <person name="Lykke-Andersen J."/>
                <person name="Garrett R.A."/>
            </authorList>
            <dbReference type="PubMed" id="9321408"/>
            <dbReference type="DOI" id="10.1093/emboj/16.20.6290"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF HIS-125</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1998" name="Science" volume="280" first="279" last="284">
            <title>Crystal structure and evolution of a transfer RNA splicing enzyme.</title>
            <authorList>
                <person name="Li H."/>
                <person name="Trotta C.R."/>
                <person name="Abelson J."/>
            </authorList>
            <dbReference type="PubMed" id="9535656"/>
            <dbReference type="DOI" id="10.1126/science.280.5361.279"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64477">
        <property type="entry name" value="G64477"/>
    </dbReference>
    <dbReference type="PDB" id="1A79">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.28 A"/>
        <property type="chains" value="A/B/C/D=9-179"/>
    </dbReference>
    <dbReference type="PDBsum" id="1A79"/>
    <dbReference type="AlphaFoldDB" id="Q58819"/>
    <dbReference type="SMR" id="Q58819"/>
    <dbReference type="STRING" id="243232.MJ_1424"/>
    <dbReference type="EnsemblBacteria" id="AAB99435">
        <property type="protein sequence ID" value="AAB99435"/>
        <property type="gene ID" value="MJ_1424"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1424"/>
    <dbReference type="eggNOG" id="arCOG01701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114393_0_0_2"/>
    <dbReference type="InParanoid" id="Q58819"/>
    <dbReference type="OMA" id="KGPGIDH"/>
    <dbReference type="PhylomeDB" id="Q58819"/>
    <dbReference type="BRENDA" id="4.6.1.16">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58819"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_01833">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
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    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="PTHR21227"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="SSF53032"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="SSF55267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000109470" description="tRNA-splicing endonuclease">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="active site">
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="active site">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Induces a strong decrease in activity." evidence="1">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="18"/>
            <end position="21"/>
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    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="24"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="36"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="47"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="64"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="85"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="110"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="127"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="157"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="169"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="9321408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1A79"/>
        </source>
    </evidence>
    <sequence length="179" mass="20664" checksum="BCC4675E4AD21BE6" modified="1996-11-01" version="1">MVRDKMGKKITGLLDGDRVIVFDKNGISKLSARHYGNVEGNFLSLSLVEALYLINLGWLEVKYKDNKPLSFEELYEYARNVEERLCLKYLVYKDLRTRGYIVKTGLKYGADFRLYERGANIDKEHSVYLVKVFPEDSSFLLSELTGFVRVAHSVRKKLLIAIVDADGDIVYYNMTYVKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58860</accession>
    <name>THRC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Threonine synthase</fullName>
            <shortName>TS</shortName>
            <ecNumber>4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">thrC</name>
        <name type="ordered locus">MJ1465</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>H2O + O-phospho-L-homoserine = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64482">
        <property type="entry name" value="H64482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870985.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58860"/>
    <dbReference type="SMR" id="Q58860"/>
    <dbReference type="STRING" id="243232.MJ_1465"/>
    <dbReference type="EnsemblBacteria" id="AAB99473">
        <property type="protein sequence ID" value="AAB99473"/>
        <property type="gene ID" value="MJ_1465"/>
    </dbReference>
    <dbReference type="GeneID" id="1452369"/>
    <dbReference type="KEGG" id="mja:MJ_1465"/>
    <dbReference type="eggNOG" id="arCOG01434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028142_4_1_2"/>
    <dbReference type="InParanoid" id="Q58860"/>
    <dbReference type="OMA" id="MWGFQAS"/>
    <dbReference type="OrthoDB" id="16594at2157"/>
    <dbReference type="PhylomeDB" id="Q58860"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14634"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019344">
        <property type="term" value="P:cysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="chain" id="PRO_0000185642" description="Threonine synthase">
        <location>
            <begin position="1"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="233"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="405" mass="44601" checksum="FAD055FB041E2DF0" modified="1996-11-01" version="1">MLQRCIKCGKTYDVDEIIYTCECGGLLEIIYDYEEIKDKVSEEKLRKREIGVWRYLEYLPVKDESKIVSLCEGGTPLYRCNNLEKELGIKELYVKNEGANPTGSFKDRGMTVGVTRANELGVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVIQVKGNFDDALDMVKQLAKEKLIYLLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVGNAGNISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFRKRAKDIIPYKNPETIATAIRIGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKKLLEEGIIDRDERIVCITTGHGLKDPDAAIRASEEPIKIECDMNVLKRILKEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58861</accession>
    <name>Y1466_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1466</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1466</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0017 and MJ0139.1.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64483">
        <property type="entry name" value="A64483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870986.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58861"/>
    <dbReference type="STRING" id="243232.MJ_1466"/>
    <dbReference type="EnsemblBacteria" id="AAB99474">
        <property type="protein sequence ID" value="AAB99474"/>
        <property type="gene ID" value="MJ_1466"/>
    </dbReference>
    <dbReference type="GeneID" id="1452370"/>
    <dbReference type="KEGG" id="mja:MJ_1466"/>
    <dbReference type="eggNOG" id="arCOG02134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079548_0_0_2"/>
    <dbReference type="InParanoid" id="Q58861"/>
    <dbReference type="OMA" id="FDFRYEK"/>
    <dbReference type="OrthoDB" id="101978at2157"/>
    <dbReference type="PhylomeDB" id="Q58861"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032874">
        <property type="entry name" value="DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13610">
        <property type="entry name" value="DDE_Tnp_IS240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107352" description="Uncharacterized protein MJ1466">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="214" mass="25626" checksum="3C6D5A76051F17EB" modified="1997-11-01" version="1">MRLTIEVIKERIVERKLFKRNRKSIEVKILAGLLYYLGLSLRKVSLFLSQFEDISHESVRIYYHKIKEVLNEPERKERNLIAIDETKLKVGDKYIYAWSAIDVETKECLGVYISKTRNYLDTILFVKSILKFCSNKPKILVDGGKWYPWALRKLGLEFERVKFGLRNCVESFFSVLKRRTKVFYNRFPNNSKFDTVISWIKSFMMFYNWMKSLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58865</accession>
    <name>Y1470_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1470</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1470</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64483">
        <property type="entry name" value="E64483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870991.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58865"/>
    <dbReference type="STRING" id="243232.MJ_1470"/>
    <dbReference type="EnsemblBacteria" id="AAB99485">
        <property type="protein sequence ID" value="AAB99485"/>
        <property type="gene ID" value="MJ_1470"/>
    </dbReference>
    <dbReference type="GeneID" id="1452375"/>
    <dbReference type="KEGG" id="mja:MJ_1470"/>
    <dbReference type="eggNOG" id="arCOG03508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG06599">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_441897_0_0_2"/>
    <dbReference type="OMA" id="IDWVFVR"/>
    <dbReference type="OrthoDB" id="20725at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018765">
        <property type="entry name" value="DUF2341"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10102">
        <property type="entry name" value="DUF2341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107356" description="Uncharacterized protein MJ1470">
        <location>
            <begin position="1"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="624" mass="72858" checksum="CEBF3B4A86A0EF80" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58879</accession>
    <name>Y1484_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1484</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1484</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64485">
        <property type="entry name" value="C64485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871006.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58879"/>
    <dbReference type="STRING" id="243232.MJ_1484"/>
    <dbReference type="EnsemblBacteria" id="AAB99499">
        <property type="protein sequence ID" value="AAB99499"/>
        <property type="gene ID" value="MJ_1484"/>
    </dbReference>
    <dbReference type="GeneID" id="1452390"/>
    <dbReference type="KEGG" id="mja:MJ_1484"/>
    <dbReference type="eggNOG" id="arCOG14906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736428_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107370" description="Uncharacterized protein MJ1484">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <sequence length="150" mass="17896" checksum="45BF3EED4000DB52" modified="1996-11-01" version="1">MEIFTIKYNASLFSNKTLRDKVYKILKENTKSYYESNSNGFFRIGGISRVDYINNNSIYFKGECKKYFGKNEVIITNFWIFKNESLADKFYNKMLKEYGNKSYIDKNPKLKYYNMSEIINYSVEMYDKDVVLIKEHTKDIKLFEVNVSMG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q58885</accession>
    <name>PYRC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroorotase</fullName>
            <shortName evidence="1">DHOase</shortName>
            <ecNumber evidence="1">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrC</name>
        <name type="ordered locus">MJ1490</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-dihydroorotate + H2O = H(+) + N-carbamoyl-L-aspartate</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64486">
        <property type="entry name" value="A64486"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871013.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58885"/>
    <dbReference type="SMR" id="Q58885"/>
    <dbReference type="STRING" id="243232.MJ_1490"/>
    <dbReference type="MEROPS" id="M38.972"/>
    <dbReference type="DNASU" id="1452397"/>
    <dbReference type="EnsemblBacteria" id="AAB99503">
        <property type="protein sequence ID" value="AAB99503"/>
        <property type="gene ID" value="MJ_1490"/>
    </dbReference>
    <dbReference type="GeneID" id="1452397"/>
    <dbReference type="KEGG" id="mja:MJ_1490"/>
    <dbReference type="eggNOG" id="arCOG00689">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015572_1_1_2"/>
    <dbReference type="InParanoid" id="Q58885"/>
    <dbReference type="OMA" id="QHAQEPR"/>
    <dbReference type="OrthoDB" id="43469at2157"/>
    <dbReference type="PhylomeDB" id="Q58885"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_A">
        <property type="entry name" value="PyrC_classI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00482">
        <property type="entry name" value="DIHYDROOROTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000147270" description="Dihydroorotase">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <sequence length="423" mass="48104" checksum="3C15A023B1E9750B" modified="1996-11-01" version="1">MLLKNCRIIKDNKIIEGDILIDENGRIKKIAKDIKVDDEIIDIKNSLVIPGVIDAHVHFRWGEEKKEDFLSGSLAGINGGVCFAIDMPNNKPPITTKELFYKKLEDCKKDSKINVFLNFGVTENNYLGTVEDAKAYKIFMVKSVGDLFIEDYSKLKDILNQNKLFCIHAEHKDVINENLKKYQLNSWIDHCKIRDEKSEVEAVKEVIKNLKIIDRQSNKKPHVHFCHISTKEALYLIKKVRQELKNIKITVEVTPHHIYLNKDMAEELKGFGKFNPPLREKDDNIALIKGIVNKDVDIIASDHAPHLLEDKLKNVKNCPSGIPGIETIVPLTLNLVNKGLISLFDAIRVLSKNPAKIFNINNKIEEGNLANLTIIDLKKEGKINAELFKSKAKFSPFDGWEVKGFPIYTVINGTLYEAYGCKC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58898</accession>
    <name>Y1503_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1503</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1503</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64487">
        <property type="entry name" value="F64487"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871026.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58898"/>
    <dbReference type="SMR" id="Q58898"/>
    <dbReference type="STRING" id="243232.MJ_1503"/>
    <dbReference type="PRIDE" id="Q58898"/>
    <dbReference type="EnsemblBacteria" id="AAB99516">
        <property type="protein sequence ID" value="AAB99516"/>
        <property type="gene ID" value="MJ_1503"/>
    </dbReference>
    <dbReference type="GeneID" id="1452410"/>
    <dbReference type="KEGG" id="mja:MJ_1503"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140786_1_0_2"/>
    <dbReference type="InParanoid" id="Q58898"/>
    <dbReference type="OMA" id="FTLETIM"/>
    <dbReference type="OrthoDB" id="96961at2157"/>
    <dbReference type="PhylomeDB" id="Q58898"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107380" description="Uncharacterized protein MJ1503">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <sequence length="132" mass="15528" checksum="44046F77C6F1D0CD" modified="1996-11-01" version="1">MSKLLLKTPCTTWTFDSLMACVFGIKVSDVKVYFDILKNGPSKINDIAERINRDRSTVQRAVQNLMNAGLVKRKQVNIKDGGYYYVYEAIPFEETKKIIKKTMEEWCNNMKKWVEELEFEDVVKEYLENIEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58901</accession>
    <name>Y1506_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1506</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1506</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64488">
        <property type="entry name" value="A64488"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58901"/>
    <dbReference type="STRING" id="243232.MJ_1506"/>
    <dbReference type="EnsemblBacteria" id="AAB99522">
        <property type="protein sequence ID" value="AAB99522"/>
        <property type="gene ID" value="MJ_1506"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1506"/>
    <dbReference type="eggNOG" id="arCOG02080">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036466_0_0_2"/>
    <dbReference type="InParanoid" id="Q58901"/>
    <dbReference type="OMA" id="IYSINAN"/>
    <dbReference type="PhylomeDB" id="Q58901"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107381" description="Uncharacterized protein MJ1506">
        <location>
            <begin position="1"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="437" mass="50390" checksum="97E6EA7694971D32" modified="1996-11-01" version="1">MLYILKCKSNYIINFHRLPQKTSKALYTNISLSFIYRFMWGIMMKKLLIILIGFILLSSISAIQIDAPQYQPNVIHPGDDVDLWIKINNDNYDNEVKNIVVEVTPHYPFELRQVNPIKGKATISHLNPGESDTVYFKLHVDENAPSRDYRIDVKVSYDEVDKEDGKETSHHYEITKIYYLHVYGIASFEINIDDTSIIPGKTKTIKLDIKNVGTGNAKYLNLYLIGNDKINILGGSLIFVGCLKANNQYIIPIKIYAVPEIEDGIYSINANLFWVGEDGKQYNSTIPLNIRVVKKIYANQPYIYLDDVKNKGDYIEITIGIANRGTTKIKHCVMTLTANGRNYTKYIGDLDEDDYDTSIFEIKEFGDIPIKVTVTYFDDYHNPYNATETFNIHVEKVKKEESLSPMYIIGGVIVVIIIILYIRKRKRHQEFEEFEEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="148" xmlns="http://uniprot.org/uniprot">
    <accession>Q58907</accession>
    <name>RGYR_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Reverse gyrase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName>Helicase</fullName>
                <ecNumber>3.6.4.12</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>Topoisomerase</fullName>
                <ecNumber>5.6.2.2</ecNumber>
            </recommendedName>
        </domain>
        <component>
            <recommendedName>
                <fullName>Mja r-Gyr intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">rgy</name>
        <name type="ordered locus">MJ1512</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue. May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Both the DNA unwinding and positive supercoiling activities require the cooperation of both domains. The cooperative action between the helicase-like and the topoisomerase domains is specific. The helicase-like domain probably does not directly unwind DNA but acts more likely by driving ATP-dependent conformational changes within the whole enzyme, functioning more like a protein motor. The 'latch' region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and therefore preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="miscellaneous">
        <text>This enzyme is the only unique feature of hyperthermophilic bacteria/archaea discovered so far. It appears to be essential for adaptation to life at high temperatures.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the prokaryotic type I/III topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12"/>
    <dbReference type="EC" id="5.6.2.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64488">
        <property type="entry name" value="G64488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871035.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58907"/>
    <dbReference type="SMR" id="Q58907"/>
    <dbReference type="STRING" id="243232.MJ_1512"/>
    <dbReference type="PRIDE" id="Q58907"/>
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        <property type="entry name" value="Hint_dom_N"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
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    <dbReference type="TIGRFAMs" id="TIGR01054">
        <property type="entry name" value="rgy"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
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        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000030353" description="Reverse gyrase, 1st part" evidence="2">
        <location>
            <begin position="1"/>
            <end position="866"/>
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    <feature type="chain" id="PRO_0000030354" description="Mja r-Gyr intein" evidence="2">
        <location>
            <begin position="867"/>
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    <feature type="chain" id="PRO_0000030355" description="Reverse gyrase, 2nd part" evidence="2">
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            <begin position="1361"/>
            <end position="1613"/>
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    <feature type="domain" description="Helicase ATP-binding">
        <location>
            <begin position="87"/>
            <end position="291"/>
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    </feature>
    <feature type="domain" description="Helicase C-terminal">
        <location>
            <begin position="310"/>
            <end position="525"/>
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    </feature>
    <feature type="domain" description="Toprim">
        <location>
            <begin position="550"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease">
        <location>
            <begin position="1070"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
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    </feature>
    <feature type="region of interest" description="Topoisomerase I">
        <location>
            <begin position="556"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAD box">
        <location>
            <begin position="203"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="active site" description="For DNA cleavage activity" evidence="1">
        <location>
            <position position="1363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="100"/>
            <end position="107"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="556"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="681"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="681"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="683"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1613" mass="188257" checksum="7170C4E120237AE3" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58915</accession>
    <name>Y1520_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1520</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1520</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the geranylgeranyl reductase family. ChlP subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G64489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871044.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
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        <property type="protein sequence ID" value="AAB99539"/>
        <property type="gene ID" value="MJ_1520"/>
    </dbReference>
    <dbReference type="GeneID" id="1452428"/>
    <dbReference type="KEGG" id="mja:MJ_1520"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_1_2"/>
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    <dbReference type="OMA" id="IGVHMDR"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
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    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000219666" description="Uncharacterized protein MJ1520">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="387" mass="43299" checksum="E0796DA99B4A9734" modified="1996-11-01" version="1">MNRNDYDVVIIGGGPVGCITGEYIKNGRVLIVEEHQSIGVPLQCAGLISKNGVKELGNPKGVVNKVRGAYIYSKNSMVKIGNEGIRAYIFERKVMDKDIAIRAAKKCDFLLKAYGKIEKDKNGYKVEITHLGEKITLNPKIIVGADGAKTITGKKLGLVNNKNREILSSCQFEMVNAEVDDDFVYIFLDRKYSERFFTWIIPMGKDRVRVGLIDRGNCYNKLIRFINENKIAKEILKNATITEFSTGSLPIGYLDKTFKDNVLLVGDAACHVKPLSGGGLYFGAMGGKIAGEVISKYLNEDIENLELYDKRWKETFGSEIKNGLRVRKLFLKLGNDTLDKIIEKLSKSDLIDYINKHGDMDRQASLSIKVLKSLDIGLGFRILRDLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58919</accession>
    <name>Y1524_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0166 protein MJ1524</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1524</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0166 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64490">
        <property type="entry name" value="C64490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871048.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58919"/>
    <dbReference type="SMR" id="Q58919"/>
    <dbReference type="STRING" id="243232.MJ_1524"/>
    <dbReference type="EnsemblBacteria" id="AAB99550">
        <property type="protein sequence ID" value="AAB99550"/>
        <property type="gene ID" value="MJ_1524"/>
    </dbReference>
    <dbReference type="GeneID" id="1452432"/>
    <dbReference type="KEGG" id="mja:MJ_1524"/>
    <dbReference type="eggNOG" id="arCOG04967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146749_2_0_2"/>
    <dbReference type="InParanoid" id="Q58919"/>
    <dbReference type="OMA" id="EHWNTLR"/>
    <dbReference type="OrthoDB" id="122737at2157"/>
    <dbReference type="PhylomeDB" id="Q58919"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003793">
        <property type="entry name" value="DUF190"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02641">
        <property type="entry name" value="DUF190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000185233" description="UPF0166 protein MJ1524">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="108" mass="12398" checksum="D3E286AFB9D4AF8B" modified="1996-11-01" version="1">MIKAKILKIYLREGDKFEGELMYKHIMKILKREGISGATVYKGICGYGVRGVAEFDIFRLSVNLPVIIECVDIEENINRVLPKLYEVIKNNGLIIITDCHVYKGETYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58927</accession>
    <name>HIS4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber>5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisA</name>
        <name type="ordered locus">MJ1532</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64491">
        <property type="entry name" value="C64491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871056.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58927"/>
    <dbReference type="SMR" id="Q58927"/>
    <dbReference type="STRING" id="243232.MJ_1532"/>
    <dbReference type="PRIDE" id="Q58927"/>
    <dbReference type="EnsemblBacteria" id="AAB99553">
        <property type="protein sequence ID" value="AAB99553"/>
        <property type="gene ID" value="MJ_1532"/>
    </dbReference>
    <dbReference type="GeneID" id="1452440"/>
    <dbReference type="KEGG" id="mja:MJ_1532"/>
    <dbReference type="eggNOG" id="arCOG00618">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_1_1_2"/>
    <dbReference type="InParanoid" id="Q58927"/>
    <dbReference type="OMA" id="EWLHLVD"/>
    <dbReference type="OrthoDB" id="89881at2157"/>
    <dbReference type="PhylomeDB" id="Q58927"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="Isoase_HisA-like_bact"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00007">
        <property type="entry name" value="TIGR00007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142092" description="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="237" mass="26132" checksum="D11E3AD24C01AC2A" modified="1996-11-01" version="1">MIIIPAVDLKDKKCVQLIQGDPNKKHLELNNPVEVAKKFVDEGAEYLHIIDLDAAFGTGNNRDVIKNIIKEVNVPVEVGGGIRNLEIAKELISLGVDRVIVGTKAILEPKFIDDLNKEIGKDKIVLAVECKEGKVVIKGWKEKVDKTPIEVIKEFEDKVGYILFTNVDVEGLLKGINVDIIKELIEKTDIPIIYSGGITTLEDIKALKELGIYGVVIGSALYKGLIDLKKALEIVKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q58976</accession>
    <name>PYRB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Aspartate carbamoyltransferase</fullName>
            <ecNumber>2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Aspartate transcarbamylase</fullName>
            <shortName>ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pyrB</name>
        <name type="ordered locus">MJ1581</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Biol. Chem." volume="275" first="15820" last="15827">
            <title>Characterization of the aspartate transcarbamoylase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Hack E.S."/>
                <person name="Vorobyova T."/>
                <person name="Sakash J.B."/>
                <person name="West J.M."/>
                <person name="Macol C.P."/>
                <person name="Herve G."/>
                <person name="Williams M.K."/>
                <person name="Kantrowitz E.R."/>
            </authorList>
            <dbReference type="PubMed" id="10748118"/>
            <dbReference type="DOI" id="10.1074/jbc.M909220199"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="Acta Crystallogr. D" volume="56" first="1061" last="1063">
            <title>Crystallization and structure determination of the catalytic trimer of Methanococcus jannaschii aspartate transcarbamoylase.</title>
            <authorList>
                <person name="Vitali J."/>
                <person name="Vorobyova T."/>
                <person name="Webster G."/>
                <person name="Kantrowitz E.R."/>
            </authorList>
            <dbReference type="PubMed" id="10944354"/>
            <dbReference type="DOI" id="10.1107/S0907444900008167"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>carbamoyl phosphate + L-aspartate = H(+) + N-carbamoyl-L-aspartate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
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        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterododecamer (2C3:3R2) of six catalytic PyrB chains organized as two trimers (C3), and six regulatory PyrI chains organized as three dimers (R2).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
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        <property type="gene ID" value="MJ_1581"/>
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        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
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        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AOTCASE"/>
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        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113247" description="Aspartate carbamoyltransferase">
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            <begin position="1"/>
            <end position="306"/>
        </location>
    </feature>
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            <begin position="7"/>
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    <feature type="helix" evidence="5">
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    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="10748118"/>
        </source>
    </evidence>
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            <dbReference type="PDB" id="4EKN"/>
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    <sequence length="306" mass="35160" checksum="CBDC31FC450CEF6A" modified="1996-11-01" version="1">MKHLISMKDIGKEEILEILDEARKMEELLNTKRPLKLLEGKILATVFYEPSTRTRLSFETAMKRLGGEVITMTDLKSSSVAKGESLIDTIRVISGYADIIVLRHPSEGAARLASEYSQVPIINAGDGSNQHPTQTLLDLYTIMREIGRIDGIKIAFVGDLKYGRTVHSLVYALSLFENVEMYFVSPKELRLPKDIIEDLKAKNIKFYEKESLDDLDDDIDVLYVTRIQKERFPDPNEYEKVKGSYKIKREYVEGKKFIIMHPLPRVDEIDYDVDDLPQAKYFKQSFYGIPVRMAILKKLIEDNEGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58987</accession>
    <name>PUR7_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber>6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">purC</name>
        <name type="ordered locus">MJ1592</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H(+) + phosphate</text>
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            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SAICAR synthetase family.</text>
    </comment>
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        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=1-242"/>
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        <property type="resolution" value="2.03 A"/>
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    <dbReference type="GeneID" id="1452500"/>
    <dbReference type="KEGG" id="mja:MJ_1592"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cobalamin biosynthetic process"/>
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        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <evidence type="ECO:0000305" key="1"/>
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    <sequence length="242" mass="27761" checksum="E72A473335DA3E1E" modified="1996-11-01" version="1">MEIKLEEILKKQPLYSGKAKSIYEIDDDKVLIEFRDDITAGNGAKHDVKQGKGYLNALISSKLFEALEENGVKTHYIKYIEPRYMIAKKVEIIPIEVIVRNIAAGSLCRRYPFEEGKELPFPIVQFDYKNDEYGDPMLNEDIAVALGLATREELNKIKEIALKVNEVLKKLFDEKGIILVDFKIEIGKDREGNLLVADEISPDTMRLWDKETRDVLDKDVFRKDLGDVIAKYRIVAERLGLL</sequence>
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<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58991</accession>
    <accession>B1PL93</accession>
    <name>AKSF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Homoisocitrate dehydrogenase</fullName>
            <shortName>HICDH</shortName>
            <ecNumber evidence="3 4">1.1.1.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Homo(2)-isocitrate/homo(3)-isocitrate dehydrogenase</fullName>
            <ecNumber evidence="3 4">1.1.1.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName>Isohomocitrate dehydrogenase</fullName>
            <shortName>IHDH</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>NAD-dependent threo-isohomocitrate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">aksF</name>
        <name type="synonym">icd</name>
        <name type="ordered locus">MJ1596</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2008" name="J. Biol. Chem." volume="283" first="28888" last="28896">
            <title>Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.</title>
            <authorList>
                <person name="Drevland R.M."/>
                <person name="Jia Y."/>
                <person name="Palmer D.R.J."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="18765671"/>
            <dbReference type="DOI" id="10.1074/jbc.M802159200"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="5013" last="5016">
            <title>Identification of enzymes homologous to isocitrate dehydrogenase that are involved in coenzyme B and leucine biosynthesis in methanoarchaea.</title>
            <authorList>
                <person name="Howell D.M."/>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10940051"/>
            <dbReference type="DOI" id="10.1128/JB.182.17.5013-5016.2000"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the NAD-dependent oxidation and decarboxylation of (2R,3S)-homoisocitrate, (2R,3S)-homo(2)-isocitrate and (2R,3S)-homo(3)-isocitrate, into 2-oxoadipate, 2-oxopimelate (2-oxoheptanedioate), and 2-oxosuberate, respectively. All these substrates are intermediates in the biosynthesis of biotin and of 7-mercaptoheptanoate, a moiety of coenzyme B in methanoarchaea (PubMed:18765671, PubMed:10940051). Is also able to produce 2-oxoazelate from (2R,3S)-homo(4)-isocitrate in vitro, but this substrate is probably not physiologically relevant (PubMed:18765671). Is unable to use any isomer of isocitrate or isopropylmalate as a substrate, and NADP as an oxidant (PubMed:10940051).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(2R,3S)-homoisocitrate + NAD(+) = 2-oxoadipate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:11900"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.87"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:11901"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(2R,3S)-iso(homo)2citrate + NAD(+) = 2-oxoheptanedioate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:68404"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:72701"/>
            <dbReference type="ChEBI" id="CHEBI:72722"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:68405"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>(2R,3S)-iso(homo)3citrate + NAD(+) = 2-oxosuberate + CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:68408"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:177881"/>
            <dbReference type="ChEBI" id="CHEBI:177882"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:68409"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">0.037 mM for (2R,3S)-homoisocitrate</KM>
            <KM evidence="3">0.016 mM for (2R,3S)-homo(2)-isocitrate</KM>
            <KM evidence="3">0.021 mM for (2R,3S)-homo(3)-isocitrate</KM>
            <Vmax evidence="3">2.6 umol/min/mg enzyme with (2R,3S)-homoisocitrate as substrate</Vmax>
            <Vmax evidence="3">8.7 umol/min/mg enzyme with (2R,3S)-homo(2)-isocitrate as substrate</Vmax>
            <Vmax evidence="3">7.1 umol/min/mg enzyme with (2R,3S)-homo(3)-isocitrate as substrate</Vmax>
        </kinetics>
        <temperatureDependence>
            <text evidence="3">Stable at 100 degrees Celsius for 10 min.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="3">Organic acid metabolism; 2-oxosuberate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.87" evidence="3 4"/>
    <dbReference type="EC" id="1.1.1.-" evidence="3 4"/>
    <dbReference type="EMBL" id="EU447775">
        <property type="protein sequence ID" value="ACA28837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58991"/>
    <dbReference type="SMR" id="Q58991"/>
    <dbReference type="STRING" id="243232.MJ_1596"/>
    <dbReference type="EnsemblBacteria" id="AAB99614">
        <property type="protein sequence ID" value="AAB99614"/>
        <property type="gene ID" value="MJ_1596"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1596"/>
    <dbReference type="eggNOG" id="arCOG01163">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031953_0_1_2"/>
    <dbReference type="InParanoid" id="Q58991"/>
    <dbReference type="OMA" id="EYDLGAR"/>
    <dbReference type="PhylomeDB" id="Q58991"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-2004"/>
    <dbReference type="BRENDA" id="4.2.1.114">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00919"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047046">
        <property type="term" value="F:homoisocitrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000083574" description="Homoisocitrate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="68"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>NADH</name>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="70"/>
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        <ligand>
            <name>(2R,3S)-homoisocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>(2R,3S)-homoisocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>(2R,3S)-homoisocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>(2R,3S)-homoisocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="2">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>(2R,3S)-homoisocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>(2R,3S)-homoisocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>(2R,3S)-homoisocitrate</name>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>NADH</name>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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            <position position="237"/>
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            <position position="241"/>
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        <location>
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            <name>NADH</name>
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            <position position="282"/>
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    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAB99614." evidence="5" ref="2">
        <original>K</original>
        <variation>Q</variation>
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
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        <source>
            <dbReference type="UniProtKB" id="Q72IW9"/>
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    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
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    </evidence>
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        <source>
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        <source>
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    <sequence length="347" mass="38536" checksum="20D7BF9FDA451116" modified="2009-03-24" version="3">MMKVCVIEGDGIGKEVIPEAIKILNELGEFEIIKGEAGLECLKKYGNALPEDTIEKAKEADIILFGAITSPKPGEVKNYKSPIITLRKMFHLYANVRPINNFGIGQLIGKIADYEFLNAKNIDIVIIRENTEDLYVGRERLENDTAIAERVITRKGSERIIRFAFEYAIKNNRKKVSCIHKANVLRITDGLFLEVFNEIKKHYNIEADDYLVDSTAMNLIKHPEKFDVIVTTNMFGDILSDEASALIGGLGLAPSANIGDDKALFEPVHGSAPDIAGKGIANPMASILSIAMLFDYIGEKEKGDLIREAVKYCLINKKVTPDLGGDLKTKDVGDEILNYIRKKLKGY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58999</accession>
    <name>K6PF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Bifunctional ADP-specific glucokinase/phosphofructokinase</fullName>
            <ecNumber>2.7.1.146</ecNumber>
            <ecNumber>2.7.1.147</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ADP-GK</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ADP-GK/PFK</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ADP-Pfk</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ADP-dependent glucokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ADP-dependent phosphofructokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pfkC</name>
        <name type="synonym">glkA</name>
        <name type="ordered locus">MJ1604</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="J. Bacteriol." volume="183" first="7145" last="7153">
            <title>ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea.</title>
            <authorList>
                <person name="Verhees C.H."/>
                <person name="Tuininga J.E."/>
                <person name="Kengen S.W.M."/>
                <person name="Stams A.J.M."/>
                <person name="van der Oost J."/>
                <person name="de Vos W.M."/>
            </authorList>
            <dbReference type="PubMed" id="11717273"/>
            <dbReference type="DOI" id="10.1128/JB.183.24.7145-7153.2001"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="12495" last="12498">
            <title>ADP-dependent glucokinase/phosphofructokinase, a novel bifunctional enzyme from the hyperthermophilic archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Sakuraba H."/>
                <person name="Yoshioka I."/>
                <person name="Koga S."/>
                <person name="Takahashi M."/>
                <person name="Kitahama Y."/>
                <person name="Satomura T."/>
                <person name="Kawakami R."/>
                <person name="Ohshima T."/>
            </authorList>
            <dbReference type="PubMed" id="11856730"/>
            <dbReference type="DOI" id="10.1074/jbc.C200059200"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>ENZYME KINETICS</scope>
    </reference>
    <comment type="function">
        <text>Catalyzes the phosphorylation of fructose 6-phosphate and D-glucose to fructose 1,6-bisphosphate and D-glucose 6-phosphate, respectively, using ADP as the phosphate donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ADP + D-glucose = AMP + D-glucose 6-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11460"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:61548"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.147"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ADP + beta-D-fructose 6-phosphate = AMP + beta-D-fructose 1,6-bisphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.146"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM>1.6 mM for D-glucose</KM>
            <KM>0.032 mM for ADP with D-glucose as phosphoryl group acceptor</KM>
            <KM>9.6 uM for fructose 6-phosphate</KM>
            <KM>0.49 mM for ADP with D-fructose 6-phosphate as phosphoryl group acceptor</KM>
            <Vmax>11.2 umol/min/mg enzyme toward fructose 6-phosphate</Vmax>
            <Vmax>9.59 umol/min/mg enzyme toward ADP</Vmax>
        </kinetics>
        <phDependence>
            <text>Optimum pH is 6.5.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; glycolysis.</text>
    </comment>
    <comment type="subunit">
        <text>Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbohydrate kinase PfkC family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.146"/>
    <dbReference type="EC" id="2.7.1.147"/>
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        <property type="protein sequence ID" value="AAB99627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C64500"/>
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    <dbReference type="PDB" id="5OD2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.98 A"/>
        <property type="chains" value="A/B/C=4-461"/>
    </dbReference>
    <dbReference type="PDBsum" id="5OD2"/>
    <dbReference type="AlphaFoldDB" id="Q58999"/>
    <dbReference type="SMR" id="Q58999"/>
    <dbReference type="STRING" id="243232.MJ_1604"/>
    <dbReference type="EnsemblBacteria" id="AAB99627">
        <property type="protein sequence ID" value="AAB99627"/>
        <property type="gene ID" value="MJ_1604"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1604"/>
    <dbReference type="eggNOG" id="arCOG03370">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046643_0_0_2"/>
    <dbReference type="InParanoid" id="Q58999"/>
    <dbReference type="OMA" id="NRIFEFR"/>
    <dbReference type="PhylomeDB" id="Q58999"/>
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        <property type="organism ID" value="3260"/>
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        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0043843">
        <property type="term" value="F:ADP-specific glucokinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_00561">
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    <dbReference type="InterPro" id="IPR011790">
        <property type="entry name" value="ADP_PFK_arc"/>
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    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
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    <dbReference type="PANTHER" id="PTHR21208">
        <property type="entry name" value="PTHR21208"/>
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    <dbReference type="Pfam" id="PF04587">
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    <dbReference type="PIRSF" id="PIRSF015883">
        <property type="entry name" value="ADP-Pfk_glckin"/>
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        <property type="entry name" value="SSF53613"/>
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    <dbReference type="TIGRFAMs" id="TIGR02045">
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    <dbReference type="PROSITE" id="PS51255">
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
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            <begin position="1"/>
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        <location>
            <begin position="361"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="391"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="394"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="406"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="419"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5OD2"/>
        </source>
    </evidence>
    <sequence length="462" mass="53362" checksum="D91088B60DBAB104" modified="1996-11-01" version="1">MCDIMEIKKFIETIKGTKLFTAYNTNVDAIKYLKDEDVQKLVDEFNHKDIIERMEEYPRIIEEPLDFVARLVHSIKTGKPAEVPIKDDKKLHEWFDRIKYDEERMGGQAGIVSNLMATLQIDKIIVYTPFLSKKQAEMFVDYDNLLYPLVENGNLVLKKVREAYRDDPIKINRIFEFKKGLKFKLNGEEITAKQSTRFIVASRPEALRIEIKDDVRKFLPKIGEAVDCAFLSGYQAIKEEYRDGKTAKYYFERAEEDIKLLKKNKNIKTHLEFASISNIEIRKMVVDYILSNVESVGMDETEIANVLHILGYDELSNNILKDSFIEDVIEGAKILLDKFKNLEVVQVHTIYYILFVCRADNPLSKEELEECLEFSTILASTKAKLGNIRAIDDLHEGLKIPHNKYGDLLKEIAEKFNDNNYKIALSPSRYVEKPKSTVGLGDTISSGAFVYYVSLLNKKRMS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q59036</accession>
    <name>Y1642_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1642</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1642</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the M.jannaschii MJ0150/MJ0739/MJ0745/MJ1460/MJ1642 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64504">
        <property type="entry name" value="H64504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871166.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59036"/>
    <dbReference type="SMR" id="Q59036"/>
    <dbReference type="STRING" id="243232.MJ_1642"/>
    <dbReference type="EnsemblBacteria" id="AAB99662">
        <property type="protein sequence ID" value="AAB99662"/>
        <property type="gene ID" value="MJ_1642"/>
    </dbReference>
    <dbReference type="GeneID" id="1452551"/>
    <dbReference type="KEGG" id="mja:MJ_1642"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128702_0_0_2"/>
    <dbReference type="InParanoid" id="Q59036"/>
    <dbReference type="OMA" id="NIGKRIC"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="PhylomeDB" id="Q59036"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019642">
        <property type="entry name" value="DUF2507"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10702">
        <property type="entry name" value="DUF2507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107455" description="Uncharacterized protein MJ1642">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="161" mass="18847" checksum="CA58BE280E7B2D17" modified="1996-11-01" version="1">MEELGRNVDVSIFRILRFMNLRKYIGIDNEMLMYYFGKELSKNLNFKNFKELSNFYKEYKLGRIEMVSENPIKIRIYDCISCSGLPDVGKPLCHFEAGFLAGYIENVFNKKCYIIETHCWGLGNKFCQFEVRLVDNKKSENCFASSGDCNGYFGKNRTKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-02-21" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q59057</accession>
    <name>MOBA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable molybdenum cofactor guanylyltransferase</fullName>
            <shortName evidence="1">MoCo guanylyltransferase</shortName>
            <ecNumber evidence="1">2.7.7.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP:molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Mo-MPT guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Molybdopterin guanylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Molybdopterin-guanine dinucleotide synthase</fullName>
            <shortName evidence="1">MGD synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mobA</name>
        <name type="ordered locus">MJ1663</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H(+) + Mo-molybdopterin = diphosphate + Mo-molybdopterin guanine dinucleotide</text>
            <dbReference type="Rhea" id="RHEA:34243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:71310"/>
            <dbReference type="EC" id="2.7.7.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MobA family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.77" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64507">
        <property type="entry name" value="E64507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871187.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59057"/>
    <dbReference type="SMR" id="Q59057"/>
    <dbReference type="STRING" id="243232.MJ_1663"/>
    <dbReference type="EnsemblBacteria" id="AAB99681">
        <property type="protein sequence ID" value="AAB99681"/>
        <property type="gene ID" value="MJ_1663"/>
    </dbReference>
    <dbReference type="GeneID" id="1452572"/>
    <dbReference type="KEGG" id="mja:MJ_1663"/>
    <dbReference type="eggNOG" id="arCOG01872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055597_2_0_2"/>
    <dbReference type="InParanoid" id="Q59057"/>
    <dbReference type="OMA" id="HENGYIE"/>
    <dbReference type="OrthoDB" id="78708at2157"/>
    <dbReference type="PhylomeDB" id="Q59057"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061603">
        <property type="term" value="F:molybdenum cofactor guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134928" description="Probable molybdenum cofactor guanylyltransferase">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00316"/>
        </source>
    </evidence>
    <sequence length="204" mass="23352" checksum="BB5C5641ACEB7E84" modified="1996-11-01" version="1">MVTIIAGIILSGGKGERIGGKKPFRVFNGKYLINYPSDILKSLNIPFVTVFAKNSIDLEMEKEYLTKYKCLISFDLIEGKGPLMGILCGMRVLNAKWFVVLPCDCPYINKEALKKLISNISIAEKNNNLCIIPKHENGYIEPLFALYKRDALSILNKIIMEDKNLSIRYFISYLNPLYIKAEELDESKRIFKNINTIEELMDNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q60276</accession>
    <name>Y3514_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL14</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks the ATP-binding site.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64511">
        <property type="entry name" value="F64511"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60276"/>
    <dbReference type="SMR" id="Q60276"/>
    <dbReference type="STRING" id="243232.MJ_ECL14"/>
    <dbReference type="EnsemblBacteria" id="AAC37087">
        <property type="protein sequence ID" value="AAC37087"/>
        <property type="gene ID" value="MJ_ECL14"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_ECL14"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q60276"/>
    <dbReference type="OMA" id="NKKGVQP"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="PhylomeDB" id="Q60276"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184678" description="Uncharacterized protein MJECL14">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="314" mass="36754" checksum="59E9992DDB441580" modified="1997-11-01" version="1">MLSFMLISESMIFTSMDNFVEALFEIDENSKEKKIKSYIESFTKGVNDIINLYYGIKIPEPILDKFFGEKEKGGLVFKFIRDLFMSLNKKGVQPVFILDELQMIKDIVMNGGKPLLKSLFQFLVSLTKERHIAHVFCLSSDSLFIEYVYNAGELEGRAKYLLVDDFDKETSLKFMDFLAVEGNINLTNEDKELIYSYVGGKAKDIKYVIEESKFKDLREVLDFMLKDEVSKLRKLLVKIKTKKIAEVEYENVVKALKLFKDNYEINEYLMDENTKEFLIKRNILFLNPVEEILKPQSYLVWNAIKRVLQNINDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q60278</accession>
    <name>Y3518_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL18</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64512">
        <property type="entry name" value="A64512"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890065.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60278"/>
    <dbReference type="SMR" id="Q60278"/>
    <dbReference type="STRING" id="243232.MJ_ECL18"/>
    <dbReference type="EnsemblBacteria" id="AAC37089">
        <property type="protein sequence ID" value="AAC37089"/>
        <property type="gene ID" value="MJ_ECL18"/>
    </dbReference>
    <dbReference type="GeneID" id="1450802"/>
    <dbReference type="KEGG" id="mja:MJ_ECL18"/>
    <dbReference type="eggNOG" id="arCOG03827">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1302633_0_0_2"/>
    <dbReference type="InParanoid" id="Q60278"/>
    <dbReference type="PhylomeDB" id="Q60278"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107506" description="Uncharacterized protein MJECL18">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <sequence length="211" mass="24214" checksum="6D4175EE98133EF5" modified="1997-11-01" version="1">MIFMFHSGAMELLKIAEKLYDKDSEKAVEVYDKAIKKAERIYDDYAKAVILSNIAKSLYSRGLTNKVIEVYNKAIKIAEESSKRDVILSKIIENLCSNRLIDKALEVVNKISDDSSKAIALSEIAKAQYNIGMHDEALKNYDKAIFITEGVFDDEIKSSILFEISRDFYNYGLVDKAFEVIGKIPYSKYRFRLLDKMAEDLHKNIKYIHGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q60282</accession>
    <name>Y3523_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL23</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64512">
        <property type="entry name" value="F64512"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890070.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60282"/>
    <dbReference type="SMR" id="Q60282"/>
    <dbReference type="STRING" id="243232.MJ_ECL23"/>
    <dbReference type="EnsemblBacteria" id="AAC37094">
        <property type="protein sequence ID" value="AAC37094"/>
        <property type="gene ID" value="MJ_ECL23"/>
    </dbReference>
    <dbReference type="GeneID" id="1450807"/>
    <dbReference type="KEGG" id="mja:MJ_ECL23"/>
    <dbReference type="eggNOG" id="arCOG02556">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_382046_0_0_2"/>
    <dbReference type="InParanoid" id="Q60282"/>
    <dbReference type="OrthoDB" id="26059at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_beta_propeller_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_supfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01011">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13360">
        <property type="entry name" value="PQQ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="SSF50998"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107511" description="Uncharacterized protein MJECL23">
        <location>
            <begin position="1"/>
            <end position="827"/>
        </location>
    </feature>
    <sequence length="827" mass="95203" checksum="6C0E929BAF645F1C" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q60292</accession>
    <name>Y3537_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL37</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64514">
        <property type="entry name" value="D64514"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890084.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60292"/>
    <dbReference type="EnsemblBacteria" id="AAC37106">
        <property type="protein sequence ID" value="AAC37106"/>
        <property type="gene ID" value="MJ_ECL37"/>
    </dbReference>
    <dbReference type="GeneID" id="1450819"/>
    <dbReference type="KEGG" id="mja:MJ_ECL37"/>
    <dbReference type="eggNOG" id="arCOG07630">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1168578_0_0_2"/>
    <dbReference type="OMA" id="SITHWEI"/>
    <dbReference type="OrthoDB" id="137350at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107520" description="Uncharacterized protein MJECL37">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <sequence length="237" mass="27184" checksum="B0DF621202CDDA11" modified="1997-11-01" version="1">MNNFSKNLEECFQKYGYENFTLLLAGYIAKYPKYEEISNNTKFLNEYHNEVSNITTCLHVYKNLAEGVKRDFNLDLLYLNDYEIKKVKSMVEPNVISDAITVVTLVNDYNNLIDAARNVKKGDKESYTKFYIALGIVVFDVILIKENVAYKVSYKLVGILISKTGFYKVIYKYGGSTALKPIESCTHWISRGEINSMPSKLYNNSDKLTNISIKINTSKLYNKSINTIRSKIMEVSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="53" xmlns="http://uniprot.org/uniprot">
    <accession>Q60311</accession>
    <name>Y3412_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS12</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64517">
        <property type="entry name" value="D64517"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60311"/>
    <dbReference type="EnsemblBacteria" id="AAC37070">
        <property type="protein sequence ID" value="AAC37070"/>
        <property type="gene ID" value="MJ_ECS12"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_ECS12"/>
    <dbReference type="eggNOG" id="arCOG02164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2748197_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107492" description="Uncharacterized protein MJECS12">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <sequence length="70" mass="8339" checksum="969A84B1E1CC3845" modified="1997-11-01" version="1">MKSKKPNFEFELKNYAIWYDINDAIIKESLCRYIKNILRKEFCGNCSKIKDIELKQKGKDIVVHLQFKLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q60314</accession>
    <name>Y006_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized oxidoreductase MJ0006</fullName>
            <ecNumber>1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0006</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
        <text evidence="1">Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64300">
        <property type="entry name" value="F64300"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869499.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60314"/>
    <dbReference type="SMR" id="Q60314"/>
    <dbReference type="STRING" id="243232.MJ_0006"/>
    <dbReference type="EnsemblBacteria" id="AAB97987">
        <property type="protein sequence ID" value="AAB97987"/>
        <property type="gene ID" value="MJ_0006"/>
    </dbReference>
    <dbReference type="GeneID" id="1450845"/>
    <dbReference type="KEGG" id="mja:MJ_0006"/>
    <dbReference type="eggNOG" id="arCOG04862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039904_0_0_2"/>
    <dbReference type="InParanoid" id="Q60314"/>
    <dbReference type="OMA" id="GHCICNS"/>
    <dbReference type="OrthoDB" id="1029at2157"/>
    <dbReference type="PhylomeDB" id="Q60314"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0500">Molybdenum</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000063231" description="Uncharacterized oxidoreductase MJ0006">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="378" mass="42051" checksum="E0C13060FF2609AA" modified="1997-11-01" version="1">MKVVHTICPGCSVGCGIDLIVKDDKVVGTYPYKRHPINEGKNCSNGKNSYKIIYHEKRLKKPLIKKNGKLVEATWDEALSFIAEKLKNYNADDITFIASGKCTNEDNYALKKLVDSLKAKIGHCICNSPKVNYAEVSTTIDDIENAKNIIIIGDVFSEHALIGRKVIKAKEKGSKVTIFNTEEKEILKLNADEFVKVDSYLGVDLSNVDKNTIIIINAPVNVDEIIKTAKENKAKVLPVAKHCNTVGATLIGIPALNKDEYFELLKNSKFLYIMGENPALVDKDVLKNVEFLVVQDIIMTETAEMADVVLPSTCWAEKDGTFINTDKRIQKINKAVNPPGDAMDDWLIIKSLAEKLGSDLGFNSLEDIQQDIHRNKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q60327</accession>
    <name>Y011_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0011</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0011</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64301">
        <property type="entry name" value="C64301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869504.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60327"/>
    <dbReference type="STRING" id="243232.MJ_0011"/>
    <dbReference type="EnsemblBacteria" id="AAB97999">
        <property type="protein sequence ID" value="AAB97999"/>
        <property type="gene ID" value="MJ_0011"/>
    </dbReference>
    <dbReference type="GeneID" id="1450850"/>
    <dbReference type="KEGG" id="mja:MJ_0011"/>
    <dbReference type="eggNOG" id="arCOG05017">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117547_0_0_2"/>
    <dbReference type="InParanoid" id="Q60327"/>
    <dbReference type="OMA" id="GCPDVPY"/>
    <dbReference type="OrthoDB" id="85068at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021778">
        <property type="entry name" value="Se/S_carrier-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11823">
        <property type="entry name" value="DUF3343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106651" description="Uncharacterized protein MJ0011">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <sequence length="197" mass="22142" checksum="7B710A00956E71F8" modified="1997-11-01" version="1">MIGKLKNLFKLGKGKKEEKAKKSLEGKGLIIFENTKDAMRAESILKDKYKIKVVAPPPEIREGCDLAIEYELIDEFGIKRELESNDIKPLKFISLNDYSLKPLELIKVKEVDGFILVRCGNMKITIDKEGNIVNISGGGCPDVPYLALKLKGRNIKDIKEEETPKNLGFTLCAYILNKGSSQRGHSWTIIDFEVLSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q60344</accession>
    <name>Y037_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0037</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0037</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64304">
        <property type="entry name" value="E64304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869528.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60344"/>
    <dbReference type="SMR" id="Q60344"/>
    <dbReference type="STRING" id="243232.MJ_0037"/>
    <dbReference type="EnsemblBacteria" id="AAB98018">
        <property type="protein sequence ID" value="AAB98018"/>
        <property type="gene ID" value="MJ_0037"/>
    </dbReference>
    <dbReference type="GeneID" id="1450875"/>
    <dbReference type="KEGG" id="mja:MJ_0037"/>
    <dbReference type="eggNOG" id="arCOG01150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075478_0_1_2"/>
    <dbReference type="InParanoid" id="Q60344"/>
    <dbReference type="OMA" id="GNHDTML"/>
    <dbReference type="OrthoDB" id="105276at2157"/>
    <dbReference type="PhylomeDB" id="Q60344"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004529">
        <property type="term" value="F:exodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004376">
        <property type="entry name" value="CHP00024_Pesterase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF5">
        <property type="entry name" value="PTHR30337:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00024">
        <property type="entry name" value="SbcD_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106661" description="Uncharacterized protein MJ0037">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <sequence length="238" mass="27684" checksum="164A1C90B8C7960B" modified="1997-11-01" version="1">MEERLKIKDFYITVDRCLVYKDYAIIADTHIGFDVFFGEGGANFPLLQKDEVIKRTLNIIDKYKINNLIINGDIKHNFKPYPKEIKFLKEFIEFLREYINVILIKGNHDTFISSAGYEIFDYFELGNYLIFHGDKEIKIDRDLLKEKFWILGHEHPSIKLRDDVGAILKFPTYLLNKNYIVLPAFNPLSPGNDLINNSASSKIIKKSYLEAEVIAITDIGLLNFGTLRDLREFAKTHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q60352</accession>
    <name>IPK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Isopentenyl phosphate kinase</fullName>
            <shortName>IPK</shortName>
            <ecNumber>2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0044</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="3192" last="3198">
            <title>Methanocaldococcus jannaschii uses a modified mevalonate pathway for biosynthesis of isopentenyl diphosphate.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="16621811"/>
            <dbReference type="DOI" id="10.1128/JB.188.9.3192-3198.2006"/>
        </citation>
        <scope>IDENTIFICATION</scope>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="ACS Chem. Biol." volume="5" first="589" last="601">
            <title>Mutation of archaeal isopentenyl phosphate kinase highlights mechanism and guides phosphorylation of additional isoprenoid monophosphates.</title>
            <authorList>
                <person name="Dellas N."/>
                <person name="Noel J.P."/>
            </authorList>
            <dbReference type="PubMed" id="20392112"/>
            <dbReference type="DOI" id="10.1021/cb1000313"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEXES WITH ISOPENTENYL PHOSPHATE; ISOPENTENYL DIPHOSPHATE AND SULFATE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF HIS-60; PHE-83; ILE-86 AND ILE-156</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the formation of isopentenyl diphosphate (IPP), the building block of all isoprenoids. Has no activity with farnesyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>ATP + isopentenyl phosphate = ADP + isopentenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2 3">4.3 uM for isopentenyl phosphate</KM>
            <KM evidence="2 3">198 uM for ATP</KM>
        </kinetics>
        <phDependence>
            <text evidence="2 3">Optimum pH is 7-9.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2 3">Retains 38% activity when incubated at 100 degrees Celsius for 10 minutes.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64305">
        <property type="entry name" value="D64305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869535.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3K4O">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A/B=1-260"/>
    </dbReference>
    <dbReference type="PDB" id="3K4Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.54 A"/>
        <property type="chains" value="A/B=1-260"/>
    </dbReference>
    <dbReference type="PDB" id="3K52">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1-260"/>
    </dbReference>
    <dbReference type="PDB" id="3K56">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.34 A"/>
        <property type="chains" value="A/B=1-260"/>
    </dbReference>
    <dbReference type="PDBsum" id="3K4O"/>
    <dbReference type="PDBsum" id="3K4Y"/>
    <dbReference type="PDBsum" id="3K52"/>
    <dbReference type="PDBsum" id="3K56"/>
    <dbReference type="AlphaFoldDB" id="Q60352"/>
    <dbReference type="SMR" id="Q60352"/>
    <dbReference type="STRING" id="243232.MJ_0044"/>
    <dbReference type="EnsemblBacteria" id="AAB98024">
        <property type="protein sequence ID" value="AAB98024"/>
        <property type="gene ID" value="MJ_0044"/>
    </dbReference>
    <dbReference type="GeneID" id="1450882"/>
    <dbReference type="KEGG" id="mja:MJ_0044"/>
    <dbReference type="eggNOG" id="arCOG00860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070213_0_0_2"/>
    <dbReference type="InParanoid" id="Q60352"/>
    <dbReference type="OMA" id="HHNASEH"/>
    <dbReference type="OrthoDB" id="96284at2157"/>
    <dbReference type="PhylomeDB" id="Q60352"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14619"/>
    <dbReference type="BRENDA" id="2.7.4.26">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q60352"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="PTHR43654:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106666" description="Isopentenyl phosphate kinase">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="6"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Abolishes enzyme activity." evidence="3">
        <original>H</original>
        <variation>A</variation>
        <variation>N</variation>
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Nearly abolishes enzyme activity." evidence="3">
        <original>H</original>
        <variation>Q</variation>
        <location>
            <position position="60"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Confers activity with farnesyl phosphate; when associated with A-86 and A-156. Confers low activity with farnesyl phosphate." evidence="3">
        <original>F</original>
        <variation>A</variation>
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Confers activity with farnesyl phosphate; when associated with A-83 and A-156." evidence="3">
        <original>I</original>
        <variation>A</variation>
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Confers low activity with farnesyl phosphate." evidence="3">
        <original>I</original>
        <variation>G</variation>
        <location>
            <position position="86"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Confers activity with farnesyl phosphate; when associated with A-83 and A-86." evidence="3">
        <original>I</original>
        <variation>A</variation>
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Confers low activity with farnesyl phosphate." evidence="3">
        <original>I</original>
        <variation>G</variation>
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="2"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="48"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="56"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="64"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="71"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="103"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="118"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="128"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="139"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="145"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="154"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="192"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="199"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="208"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="217"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="231"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="242"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="253"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16621811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="20392112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3K4O"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3K4Y"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="3K56"/>
        </source>
    </evidence>
    <sequence length="260" mass="29472" checksum="833C2BFF020E3F07" modified="1997-11-01" version="1">MLTILKLGGSILSDKNVPYSIKWDNLERIAMEIKNALDYYKNQNKEIKLILVHGGGAFGHPVAKKYLKIEDGKKIFINMEKGFWEIQRAMRRFNNIIIDTLQSYDIPAVSIQPSSFVVFGDKLIFDTSAIKEMLKRNLVPVIHGDIVIDDKNGYRIISGDDIVPYLANELKADLILYATDVDGVLIDNKPIKRIDKNNIYKILNYLSGSNSIDVTGGMKYKIDMIRKNKCRGFVFNGNKANNIYKALLGEVEGTEIDFSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q60355</accession>
    <name>Y047_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0047</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0047</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869538.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60355"/>
    <dbReference type="SMR" id="Q60355"/>
    <dbReference type="STRING" id="243232.MJ_0047"/>
    <dbReference type="EnsemblBacteria" id="AAB98027">
        <property type="protein sequence ID" value="AAB98027"/>
        <property type="gene ID" value="MJ_0047"/>
    </dbReference>
    <dbReference type="GeneID" id="1450885"/>
    <dbReference type="KEGG" id="mja:MJ_0047"/>
    <dbReference type="eggNOG" id="arCOG00541">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="InParanoid" id="Q60355"/>
    <dbReference type="OMA" id="FSCHAGM"/>
    <dbReference type="OrthoDB" id="39954at2157"/>
    <dbReference type="PhylomeDB" id="Q60355"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16661">
        <property type="entry name" value="Lactamase_B_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106668" description="Uncharacterized protein MJ0047">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="428" mass="48492" checksum="A649ED66705AA01B" modified="1998-12-15" version="2">MEIIFRGAALEVGRSCIEIKTDKSKILLDCGVKLGKEIEYPILDNSIRDVDKVFISHAHLDHSGALPVLFHRKMDVPVITTELSKKLIKVLLKDMVKIAETENKKIPYNNHDVKEAIRHTIPLNYNDKKYYKDFSYELFSAGHIPGSASILLNYQNNKTILYTGDVKLRDTRLTKGADLSYTKDDIDILIIESTYGNSIHPDRKAVELSFIEKIKEILFRGGVALIPVFAVDRAQEILLILNDYNIDAPIYLDGMAVEVTKLMLNYKHMLNESSQLEKALKNVKIIEKSEDRIKAIENLSKNGGIVVTTAGMLDGGPILYYLKLFMHNPKNALLLTGYQVRDSNGRHLIETGKIFIGKDEIKPNLEVCMYNFSCHAGMDELHEIIKKVNPELLIIQHGEEVQATILRNWALEHGFDAITPKLGEKIRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q60370</accession>
    <name>Y065_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0065</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0065</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64308">
        <property type="entry name" value="A64308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869557.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60370"/>
    <dbReference type="STRING" id="243232.MJ_0065"/>
    <dbReference type="EnsemblBacteria" id="AAB98045">
        <property type="protein sequence ID" value="AAB98045"/>
        <property type="gene ID" value="MJ_0065"/>
    </dbReference>
    <dbReference type="GeneID" id="1450904"/>
    <dbReference type="KEGG" id="mja:MJ_0065"/>
    <dbReference type="eggNOG" id="arCOG03231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038447_0_0_2"/>
    <dbReference type="InParanoid" id="Q60370"/>
    <dbReference type="OMA" id="TTTICGD"/>
    <dbReference type="OrthoDB" id="49106at2157"/>
    <dbReference type="PhylomeDB" id="Q60370"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012032">
        <property type="entry name" value="UCP006598"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09890">
        <property type="entry name" value="DUF2117"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006598">
        <property type="entry name" value="UCP006598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106676" description="Uncharacterized protein MJ0065">
        <location>
            <begin position="1"/>
            <end position="363"/>
        </location>
    </feature>
    <sequence length="363" mass="40405" checksum="F56B42C9C1F4D5C5" modified="1997-11-01" version="1">MRIGVVIHGPEIIDSGYALKIINLLKKFGEVKAKLGGTMGRVAVIDNNLQDIIDISEKLMPSQSLKKLANNDILILMNYGKSKITGHTFGKIVVERANLNKPIIQIERPGEEDGTIIIWNDDNSKIVKEIANYLSKELNLKIEKCISNGLEVWEKEGRVFRKVHGVDVGEAILVNGIVVGKAKSNEVILIAENGKLVDIIGGELKEGGIEKLKNVDLKKAVIKTGILRRHPTNPKIESKEIDEGYTIIINHAGEDVIEMIKNKGVVAVITIGDDTTTICGDILARFGIKIIGITDGDRDEILKNPVILKGSVIFLIKNMRDDDVGRILEKNLNLNKKYCYQELLDEVKKIFNDNNICYEEFVY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q60374</accession>
    <name>RELE1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative toxin RelE1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">relE1</name>
        <name type="ordered locus">MJ0071</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. Its cognate antitoxin is RelB1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelE toxin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64308">
        <property type="entry name" value="G64308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869563.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60374"/>
    <dbReference type="SMR" id="Q60374"/>
    <dbReference type="STRING" id="243232.MJ_0071"/>
    <dbReference type="EnsemblBacteria" id="AAB98049">
        <property type="protein sequence ID" value="AAB98049"/>
        <property type="gene ID" value="MJ_0071"/>
    </dbReference>
    <dbReference type="GeneID" id="1450910"/>
    <dbReference type="KEGG" id="mja:MJ_0071"/>
    <dbReference type="eggNOG" id="arCOG01665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155761_6_2_2"/>
    <dbReference type="InParanoid" id="Q60374"/>
    <dbReference type="OMA" id="FRVQYVV"/>
    <dbReference type="OrthoDB" id="125851at2157"/>
    <dbReference type="PhylomeDB" id="Q60374"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000106679" description="Putative toxin RelE1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="90" mass="10851" checksum="78902F645671F70A" modified="1997-11-01" version="1">MKFNVEIHKRVLKDLKDLPPSNLKKFKELIETLKTNPIPKEKFDIKRLKGSDEVYRVRIGKFRVQYVVLWDDRIIIIRKISRREGAYKNP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2022-05-25" version="15" xmlns="http://uniprot.org/uniprot">
    <accession>P0CW39</accession>
    <name>VAPB5_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative antitoxin VapB5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vapB5</name>
        <name type="ordered locus">MJ1473.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>IDENTIFICATION</scope>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Possibly the antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is VapC5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P0CW39"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000408089" description="Putative antitoxin VapB5">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="107" mass="11649" checksum="7A18906E90BFAC8E" modified="2011-05-03" version="1">MQGPVIIPLISTLGLSFLAILLAYKISFSVIGFINSTLPTTLFPSKPYMLFVKISTISPLTCPSLIILTPALTWSLTALSMAYLYSSYKPNTFFTLSKNVSSFLTTG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>P54015</accession>
    <name>RL4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl4</name>
        <name type="ordered locus">MJ0177</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64322">
        <property type="entry name" value="B64322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869672.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54015"/>
    <dbReference type="SMR" id="P54015"/>
    <dbReference type="STRING" id="243232.MJ_0177"/>
    <dbReference type="EnsemblBacteria" id="AAB98162">
        <property type="protein sequence ID" value="AAB98162"/>
        <property type="gene ID" value="MJ_0177"/>
    </dbReference>
    <dbReference type="GeneID" id="1451024"/>
    <dbReference type="KEGG" id="mja:MJ_0177"/>
    <dbReference type="eggNOG" id="arCOG04071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026535_0_0_2"/>
    <dbReference type="InParanoid" id="P54015"/>
    <dbReference type="OMA" id="WHQKVNV"/>
    <dbReference type="OrthoDB" id="65833at2157"/>
    <dbReference type="PhylomeDB" id="P54015"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_L4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_L4/L1e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_L4/L1e_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_L4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_L4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_L4-archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="PTHR19431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="SSF52166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000129332" description="50S ribosomal protein L4">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <sequence length="252" mass="27810" checksum="2C5E8C78F5C0BCF0" modified="1996-10-01" version="1">MKAVVYNLNGEAVKEIDLPAVFEEEYRPDLIKRAFLSAFTARLQPKGSDPLAGLRTSAKNIGKGHGRARVDRVPQGWAARVPQAVGGRRAHPPKVEKILWERVNKKERIKAIKSAIAATANPELVKERGHVFETENLPIIVESSFEELQKTKDVFAVFEKLGISDDVIRAKNGIKIRAGKGKMRGRKYKKPRSILVVVGDKCNAILASRNLPGVDVITAKDLGIIHLAPGGVAGRLTVWTESALEKLKERFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>P54058</accession>
    <name>RL40_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L40e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl40e</name>
        <name type="ordered locus">MJ0707</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64388">
        <property type="entry name" value="C64388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870213.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54058"/>
    <dbReference type="SMR" id="P54058"/>
    <dbReference type="STRING" id="243232.MJ_0707"/>
    <dbReference type="EnsemblBacteria" id="AAB98701">
        <property type="protein sequence ID" value="AAB98701"/>
        <property type="gene ID" value="MJ_0707"/>
    </dbReference>
    <dbReference type="GeneID" id="8805340"/>
    <dbReference type="KEGG" id="mja:MJ_0707"/>
    <dbReference type="eggNOG" id="arCOG04049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_205640_0_0_2"/>
    <dbReference type="InParanoid" id="P54058"/>
    <dbReference type="OrthoDB" id="127293at2157"/>
    <dbReference type="PhylomeDB" id="P54058"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="L40e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_L40e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_L40e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="PTHR39649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000138781" description="50S ribosomal protein L40e">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <sequence length="47" mass="5618" checksum="3C79E7CBCE9341E3" modified="1996-10-01" version="1">MPFEEAMKRLFMKKICMRCNARNPWRATKCRKCGYKGLRPKAKEPRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>P54060</accession>
    <name>RL15E_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L15e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl15e</name>
        <name type="ordered locus">MJ0983</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64422">
        <property type="entry name" value="G64422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870497.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54060"/>
    <dbReference type="SMR" id="P54060"/>
    <dbReference type="STRING" id="243232.MJ_0983"/>
    <dbReference type="EnsemblBacteria" id="AAB98986">
        <property type="protein sequence ID" value="AAB98986"/>
        <property type="gene ID" value="MJ_0983"/>
    </dbReference>
    <dbReference type="GeneID" id="8805475"/>
    <dbReference type="KEGG" id="mja:MJ_0983"/>
    <dbReference type="eggNOG" id="arCOG04209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080796_1_0_2"/>
    <dbReference type="InParanoid" id="P54060"/>
    <dbReference type="OMA" id="KYMQELY"/>
    <dbReference type="OrthoDB" id="85023at2157"/>
    <dbReference type="PhylomeDB" id="P54060"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00256">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_L15e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020926">
        <property type="entry name" value="Ribosomal_L15e_arc"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_L15e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="PTHR11847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000127573" description="50S ribosomal protein L15e">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="194" mass="22578" checksum="8FAEB77FEA99BF5C" modified="1996-10-01" version="1">MGIYKYIREAWKRPKESYVRQLLWERLQQWRREPAVVRIERPTRLDRARALGYKPKQGIIVVRVRVRRGGLRKPRPKNSKKPATLGVNKITMGKSIQRIAEERAARKYPNMEVLNSYWVGEDGKHKWYEVILVDPYHPAIKADPQLNWLCTGKHRGRAFRGLTSAGKKGRGLRNKGIGAEKVRPSIRAHGRRGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>P54109</accession>
    <name>RS2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>30S ribosomal protein S2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rps2</name>
        <name type="ordered locus">MJ0982</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870496.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54109"/>
    <dbReference type="SMR" id="P54109"/>
    <dbReference type="STRING" id="243232.MJ_0982"/>
    <dbReference type="EnsemblBacteria" id="AAB98985">
        <property type="protein sequence ID" value="AAB98985"/>
        <property type="gene ID" value="MJ_0982"/>
    </dbReference>
    <dbReference type="GeneID" id="1451880"/>
    <dbReference type="KEGG" id="mja:MJ_0982"/>
    <dbReference type="eggNOG" id="arCOG04245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058171_3_0_2"/>
    <dbReference type="InParanoid" id="P54109"/>
    <dbReference type="OMA" id="QQKTKDM"/>
    <dbReference type="OrthoDB" id="63341at2157"/>
    <dbReference type="PhylomeDB" id="P54109"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01425">
        <property type="entry name" value="RPS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00291_A">
        <property type="entry name" value="Ribosomal_S2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001865">
        <property type="entry name" value="Ribosomal_S2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023454">
        <property type="entry name" value="Ribosomal_S2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018130">
        <property type="entry name" value="Ribosomal_S2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005707">
        <property type="entry name" value="Ribosomal_S2_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023591">
        <property type="entry name" value="Ribosomal_S2_flav_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11489">
        <property type="entry name" value="PTHR11489"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00318">
        <property type="entry name" value="Ribosomal_S2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00395">
        <property type="entry name" value="RIBOSOMALS2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52313">
        <property type="entry name" value="SSF52313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01012">
        <property type="entry name" value="uS2_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00963">
        <property type="entry name" value="RIBOSOMAL_S2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000134323" description="30S ribosomal protein S2">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="222" mass="25708" checksum="0271F5EAC0127616" modified="1998-07-15" version="2">MSEKELLVPLDTYLASGIHIGTQQKTKDMEKFIYRVRSDGLYVLDVRKTDERLRIAAKFLARYEPEDILAVSRRIYTMGPLEEFGKYTGIRTVAGRFVPGTLTNPAYKGFMEPEVVFISDPRVDRQALKEATEIGVPIVGLCDTEHLTSFIDLVIPTNNKGKKAVALIYYLLTREYLKNRGVITDDTKLPFTYEEFLEKAANPKYRIIIQPKDKRRRRRRRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>P81305</accession>
    <name>Y22B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0226.2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0226.2</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81305"/>
    <dbReference type="STRING" id="243232.MJ_0226.2"/>
    <dbReference type="EnsemblBacteria" id="AAB98218">
        <property type="protein sequence ID" value="AAB98218"/>
        <property type="gene ID" value="MJ_0226.2"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0226.2"/>
    <dbReference type="eggNOG" id="arCOG09659">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021520_0_0_2"/>
    <dbReference type="OMA" id="RYIVEIY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106749" description="Uncharacterized protein MJ0226.2">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="122" mass="14629" checksum="87ED41E0ABD17C46" modified="1998-07-15" version="1">MGTMNVVGLAIYLYAGFLSFIFGFISLYAFIKYSKLERKNKEIIYVYVDNLKNFSPYMFYSILLTFTIVFVIFALFICFVFNFDLYLGLTMLFSYFLIIYLFCFKKYRVEIYKDGFYGVFQN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>P81323</accession>
    <name>Y83B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0333 protein MJ0835.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0835.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0333 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81323"/>
    <dbReference type="SMR" id="P81323"/>
    <dbReference type="STRING" id="243232.MJ_0835.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98841">
        <property type="protein sequence ID" value="AAB98841"/>
        <property type="gene ID" value="MJ_0835.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0835.1"/>
    <dbReference type="eggNOG" id="arCOG05055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143949_0_0_2"/>
    <dbReference type="OMA" id="YAGMTWD"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000218267" description="UPF0333 protein MJ0835.1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="130" mass="14578" checksum="212874E92CA64C33" modified="1998-07-15" version="1">MNTMENKIIKSKKAQVSLEFSFLFLAILLASIITISHFLSQNFTKDDKVISDVENAAKTAVILANSGYNGINPNVTLIYGGISWSGNKKNIYIYISPKSYITPEIKNFIVSYIYNVTKINQSEYNITVNP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q57650</accession>
    <name>RPO6_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo6</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit K</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo6</name>
        <name evidence="1 2" type="synonym">rpoK</name>
        <name type="ordered locus">MJ0197</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64324">
        <property type="entry name" value="F64324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869692.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57650"/>
    <dbReference type="SMR" id="Q57650"/>
    <dbReference type="STRING" id="243232.MJ_0197"/>
    <dbReference type="EnsemblBacteria" id="AAB98177">
        <property type="protein sequence ID" value="AAB98177"/>
        <property type="gene ID" value="MJ_0197"/>
    </dbReference>
    <dbReference type="GeneID" id="1451046"/>
    <dbReference type="KEGG" id="mja:MJ_0197"/>
    <dbReference type="eggNOG" id="arCOG01268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112527_5_1_2"/>
    <dbReference type="InParanoid" id="Q57650"/>
    <dbReference type="OMA" id="ATIETKC"/>
    <dbReference type="OrthoDB" id="126193at2157"/>
    <dbReference type="PhylomeDB" id="Q57650"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00192">
        <property type="entry name" value="RNApol_arch_Rpo6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="SSF63562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000133813" description="DNA-directed RNA polymerase subunit Rpo6">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="8688087"/>
        </source>
    </evidence>
    <sequence length="57" mass="6292" checksum="46F97B2F02416C77" modified="1997-11-01" version="1">MKLTKFEIARILGARSLQISSGAYATIETKCDSSLKIAYEEIKQGKVPLKPIRPVKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q57657</accession>
    <name>PUR1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Amidophosphoribosyltransferase</fullName>
            <shortName evidence="2">ATase</shortName>
            <ecNumber evidence="2">2.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Glutamine phosphoribosylpyrophosphate amidotransferase</fullName>
            <shortName evidence="2">GPATase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purF</name>
        <name type="ordered locus">MJ0204</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine</text>
            <dbReference type="Rhea" id="RHEA:14905"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="EC" id="2.4.2.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.14" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64325">
        <property type="entry name" value="E64325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869699.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57657"/>
    <dbReference type="SMR" id="Q57657"/>
    <dbReference type="STRING" id="243232.MJ_0204"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="AAB98188">
        <property type="protein sequence ID" value="AAB98188"/>
        <property type="gene ID" value="MJ_0204"/>
    </dbReference>
    <dbReference type="GeneID" id="1451053"/>
    <dbReference type="KEGG" id="mja:MJ_0204"/>
    <dbReference type="eggNOG" id="arCOG00093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022389_3_1_2"/>
    <dbReference type="InParanoid" id="Q57657"/>
    <dbReference type="OMA" id="ENAQPTF"/>
    <dbReference type="OrthoDB" id="37299at2157"/>
    <dbReference type="PhylomeDB" id="Q57657"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00124"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004044">
        <property type="term" value="F:amidophosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00715">
        <property type="entry name" value="GPATase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01931">
        <property type="entry name" value="PurF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005854">
        <property type="entry name" value="PurF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035584">
        <property type="entry name" value="PurF_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000485">
        <property type="entry name" value="Amd_phspho_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01134">
        <property type="entry name" value="purF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000139644" description="Amidophosphoribosyltransferase">
        <location>
            <begin position="2"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="450"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01931"/>
        </source>
    </evidence>
    <sequence length="471" mass="52187" checksum="27E25B66A8D5120A" modified="2007-01-23" version="3">MCGIFGIYSYERLNVAKKIYYGLFALQHRGQEGAGIATSDGKNIHYYKNIGLVTDVFKNETLQNLFGYIGIGHVRYSTTGGKAVENCQPFVVKSSFGNIAIAHNGDLVNSDELRRELEMKGHIFTSSTDSEVIAQLLVRELLKTSDKIEAIKNTLKKLVGAYSLLIMFNDSLIAVRDPWGFKPLCIGRDESNIYISSEDCALTTLDAEFVKDIEPGEIIEIKDGEIISHKLDYGVSEYNPVNVDVPCIYRGAATCMFEYVYFARPDSTIDGISVYKVRKRIGKILAKEHPVDADVVSPIPDSGVTFALGFSEESGIPYYEGLIKNRYVGRTFILPSQNERELAVRLKLSPVKSVLEGKRVVLVDDSIVRGTTSRRIVNMVRKAGAKEVHLRIGCPKIISPCYYGIDMATKKELIASNKTEEEIGKAIGVDSIGYLSLEGLVKAIGRKDLCLACVTGKYPTEVNFEKILGRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q57675</accession>
    <name>VATI_METJA</name>
    <protein>
        <recommendedName>
            <fullName>V-type ATP synthase subunit I</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>V-ATPase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">atpI</name>
        <name type="ordered locus">MJ0222</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64327">
        <property type="entry name" value="G64327"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57675"/>
    <dbReference type="SMR" id="Q57675"/>
    <dbReference type="STRING" id="243232.MJ_0222"/>
    <dbReference type="EnsemblBacteria" id="AAB98208">
        <property type="protein sequence ID" value="AAB98208"/>
        <property type="gene ID" value="MJ_0222"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0222"/>
    <dbReference type="eggNOG" id="arCOG04138">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025558_1_0_2"/>
    <dbReference type="InParanoid" id="Q57675"/>
    <dbReference type="OMA" id="MAVNIMA"/>
    <dbReference type="PhylomeDB" id="Q57675"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="PTHR11629"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000119229" description="V-type ATP synthase subunit I">
        <location>
            <begin position="1"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="425"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="497"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="534"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="556"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="605"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="695" mass="76954" checksum="F03E5CEBEE29D53D" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q57677</accession>
    <name>Y224_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0224</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0224</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64328">
        <property type="entry name" value="A64328"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57677"/>
    <dbReference type="STRING" id="243232.MJ_0224"/>
    <dbReference type="PRIDE" id="Q57677"/>
    <dbReference type="EnsemblBacteria" id="AAB98209">
        <property type="protein sequence ID" value="AAB98209"/>
        <property type="gene ID" value="MJ_0224"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0224"/>
    <dbReference type="eggNOG" id="arCOG04364">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108786_0_0_2"/>
    <dbReference type="InParanoid" id="Q57677"/>
    <dbReference type="OMA" id="RAAPICY"/>
    <dbReference type="PhylomeDB" id="Q57677"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007501">
        <property type="entry name" value="DUF531"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04407">
        <property type="entry name" value="DUF531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006006">
        <property type="entry name" value="UCP006006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106747" description="Uncharacterized protein MJ0224">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <sequence length="169" mass="19283" checksum="F24320F7DCA02BBF" modified="1996-11-01" version="1">MKKLKRLTLILYNSYDKTRWHEAHKRAIARAAPICYAFDCNLAIMDFPCKMEDILNIKTTIGNSGEYLEKLIEKNRFFIVDKFLPQFGIPIASTSKPDEKKAITPLDTAYLLKKKPIGVYVGLGRHGLPKDIMESCVYHLDVTEKRVSLETCTAIGSIPAVIYCYTKYI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57702</accession>
    <name>RADB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>DNA repair and recombination protein RadB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">radB</name>
        <name type="ordered locus">MJ0254</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic RecA-like protein family. RadB subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64331">
        <property type="entry name" value="G64331"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869752.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57702"/>
    <dbReference type="SMR" id="Q57702"/>
    <dbReference type="STRING" id="243232.MJ_0254"/>
    <dbReference type="EnsemblBacteria" id="AAB98241">
        <property type="protein sequence ID" value="AAB98241"/>
        <property type="gene ID" value="MJ_0254"/>
    </dbReference>
    <dbReference type="GeneID" id="1451108"/>
    <dbReference type="KEGG" id="mja:MJ_0254"/>
    <dbReference type="eggNOG" id="arCOG00417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_2_0_2"/>
    <dbReference type="InParanoid" id="Q57702"/>
    <dbReference type="OMA" id="GNTLEHW"/>
    <dbReference type="OrthoDB" id="93716at2157"/>
    <dbReference type="PhylomeDB" id="Q57702"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00350">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="DNA_recomb/repair_Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011939">
        <property type="entry name" value="DNA_repair_and_recomb_RadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003336">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02237">
        <property type="entry name" value="recomb_radB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150113" description="DNA repair and recombination protein RadB">
        <location>
            <begin position="1"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="20"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="212" mass="23842" checksum="0BD1C9D5D68579CB" modified="1996-11-01" version="1">MLKEILLGNAEKGIITQIYGPPGVGKTNICIINSINAVNSGKVIYIDTEGGLSIERIKQIASNNYKIVLENMIIYNAFDFYEQDKIIQKELPLITNNASLIVVDNITSLYRLELSDEANKNIMLNKMLGNQVKTLLKLAKTNNLAVIITNQVRETVNGFEASGGRLLEYWSKCIVRLEKLNGDRLAILEKHLHAGEERVKFRIVERGIEIID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57715</accession>
    <name>PORA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Pyruvate synthase subunit PorA</fullName>
            <ecNumber>1.2.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Pyruvate oxidoreductase alpha chain</fullName>
            <shortName>POR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Pyruvic-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">porA</name>
        <name type="ordered locus">MJ0267</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>CoA + 2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate = acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64333">
        <property type="entry name" value="D64333"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57715"/>
    <dbReference type="SMR" id="Q57715"/>
    <dbReference type="STRING" id="243232.MJ_0267"/>
    <dbReference type="EnsemblBacteria" id="AAB98254">
        <property type="protein sequence ID" value="AAB98254"/>
        <property type="gene ID" value="MJ_0267"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0267"/>
    <dbReference type="eggNOG" id="arCOG01608">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002569_5_0_2"/>
    <dbReference type="InParanoid" id="Q57715"/>
    <dbReference type="OMA" id="WNDQQDS"/>
    <dbReference type="PhylomeDB" id="Q57715"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000099896" description="Pyruvate synthase subunit PorA">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <sequence length="389" mass="42971" checksum="2CAA3DDE3EB0F5A9" modified="1996-11-01" version="1">METMCEVKVITGTSAAAEAAKLADVDVIAAYPITPQTTCVEKLAEFVANGELDAEYIKVESEHSAMSACIGAAATGARTFTATASQGLALMHEMLFIASGMRLPIVMMVANRALSAPINIWNDHQDSIAERDSGWIQIYVEDNQETLDSIIQAYKIAENEDVLLPVMVCLDGFILTHTVEPVTIPKAERVREFLGVYEPKHAYLDPDRPITQGPVGVPDCYMETRKQIEEAMERAKKVIRDVNEEFAEWFKRKYGNGLVEAYNLDNADTVLVAMGSVCGTIKYVIDELKKEGKNVGLLRIRAFRPFPKEDVKELLKDANNIAVLDKNISLGFNKGALGIEMASILKNKKVCNYIVGLGGRDIKIDDIKTIINHVEKAEDDSTLWVGLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q57723</accession>
    <name>Y275_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0275</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0275</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98262.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64334">
        <property type="entry name" value="D64334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57723"/>
    <dbReference type="STRING" id="243232.MJ_0275"/>
    <dbReference type="EnsemblBacteria" id="AAB98262">
        <property type="protein sequence ID" value="AAB98262"/>
        <property type="gene ID" value="MJ_0275"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0275"/>
    <dbReference type="eggNOG" id="arCOG08265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1357933_0_0_2"/>
    <dbReference type="OMA" id="ISYCYYR"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106768" description="Uncharacterized protein MJ0275">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="204" mass="24482" checksum="63E81963985BFA1C" modified="1996-11-01" version="1">MSMKKVSIIVLFILSLILTISICGCFENEKEEVNKPNMTVIEKENIKVQNNKPIENLKEESCKLNITNDENRTNNITKTTKKYDFSKPVDMDKMFLNLPYNEETDFDDKIIKKYKNITFVVSRKPIDLSYAYIYNEVKEYPERDIFGNYIYYEFIPKNANLSISYCYYRKVGNYYIIMQTYEKSRKANDLWMNWTKYVFSLFEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q57748</accession>
    <name>Y300_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0300</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0300</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64337">
        <property type="entry name" value="E64337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869798.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57748"/>
    <dbReference type="SMR" id="Q57748"/>
    <dbReference type="STRING" id="243232.MJ_0300"/>
    <dbReference type="PRIDE" id="Q57748"/>
    <dbReference type="DNASU" id="1451155"/>
    <dbReference type="EnsemblBacteria" id="AAB98287">
        <property type="protein sequence ID" value="AAB98287"/>
        <property type="gene ID" value="MJ_0300"/>
    </dbReference>
    <dbReference type="GeneID" id="1451155"/>
    <dbReference type="KEGG" id="mja:MJ_0300"/>
    <dbReference type="eggNOG" id="arCOG00224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039613_6_1_2"/>
    <dbReference type="InParanoid" id="Q57748"/>
    <dbReference type="OMA" id="IEGPCHH"/>
    <dbReference type="OrthoDB" id="47614at2157"/>
    <dbReference type="PhylomeDB" id="Q57748"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000105831" description="Uncharacterized HTH-type transcriptional regulator MJ0300">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="HTH lysR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="296" mass="33111" checksum="13474BFF48985038" modified="1996-11-01" version="1">MDPKISYFQTFIVASKTKSFSKAAKRLGITQGTVSNHISALEKYFDAQLFLRTPEGVDLTPEGKIFYERAEKILDLLNEAKLLMRAIHENPEGIIRIYASTTPGEHILPSIIKEYKSSYKNVDFEITITDSERCFKALDEGLADIAAVGYLKNKNYEYTIIGKDRLVLIVPPNHPLAEKGTAKLEDILKEDYIDREEGSGTREAFIKALNDKGYSIMDLNVVMRLGSHSAVITAVSEGYGVSVVSEIPAKKAEDAGLIKIVPVVDLDVVRYLYLVKSRRPKNPSAVKSFWEFVTKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57750</accession>
    <name>HIS2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName>PRA-PH</shortName>
            <ecNumber>3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisE</name>
        <name type="ordered locus">MJ0302</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PRA-PH family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64337">
        <property type="entry name" value="G64337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869800.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57750"/>
    <dbReference type="SMR" id="Q57750"/>
    <dbReference type="STRING" id="243232.MJ_0302"/>
    <dbReference type="DNASU" id="1451157"/>
    <dbReference type="EnsemblBacteria" id="AAB98289">
        <property type="protein sequence ID" value="AAB98289"/>
        <property type="gene ID" value="MJ_0302"/>
    </dbReference>
    <dbReference type="GeneID" id="1451157"/>
    <dbReference type="KEGG" id="mja:MJ_0302"/>
    <dbReference type="eggNOG" id="arCOG02677">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123337_1_2_2"/>
    <dbReference type="InParanoid" id="Q57750"/>
    <dbReference type="OMA" id="FTHEKGE"/>
    <dbReference type="OrthoDB" id="110768at2157"/>
    <dbReference type="PhylomeDB" id="Q57750"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136391" description="Phosphoribosyl-ATP pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="95" mass="11181" checksum="7A7853EE3171919E" modified="1996-11-01" version="1">MILEEVYEIIKQRIKEKPEGSYVAKLTTDDKKTAINKICEKIGEESTELILAAKDDKKDEIIYEAADLIFHTMVLLAYKNIEFEELLKEFERRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q57766</accession>
    <name>FTR_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formylmethanofuran--tetrahydromethanopterin formyltransferase</fullName>
            <shortName evidence="1">Ftr</shortName>
            <ecNumber evidence="1">2.3.1.101</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">H4MPT formyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftr</name>
        <name type="ordered locus">MJ0318</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible transfer of a formyl group from formylmethanofuran (formyl-MFR) to tetrahydromethanopterin (H(4)MPT) to produce 5-formyl tetrahydromethanopterin (5-formyl-H(4)MPT) and methanofuran (MFR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,6,7,8-tetrahydromethanopterin + H(+) + N-formylmethanofuran = methanofuran + N(5)-formyl-5,6,7,8-tetrahydromethanopterin</text>
            <dbReference type="Rhea" id="RHEA:18061"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="2.3.1.101"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FTR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.101" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64339">
        <property type="entry name" value="G64339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869816.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57766"/>
    <dbReference type="SMR" id="Q57766"/>
    <dbReference type="STRING" id="243232.MJ_0318"/>
    <dbReference type="EnsemblBacteria" id="AAB98303">
        <property type="protein sequence ID" value="AAB98303"/>
        <property type="gene ID" value="MJ_0318"/>
    </dbReference>
    <dbReference type="GeneID" id="1451173"/>
    <dbReference type="KEGG" id="mja:MJ_0318"/>
    <dbReference type="eggNOG" id="arCOG02695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081314_0_0_2"/>
    <dbReference type="InParanoid" id="Q57766"/>
    <dbReference type="OMA" id="VIMCPAE"/>
    <dbReference type="OrthoDB" id="62652at2157"/>
    <dbReference type="PhylomeDB" id="Q57766"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00693"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030270">
        <property type="term" value="F:formylmethanofuran-tetrahydromethanopterin N-formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.520">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00579">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014053">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002770">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023447">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_fd-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022667">
        <property type="entry name" value="ForMFR_H4MPT_ForTrfase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01913">
        <property type="entry name" value="FTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02741">
        <property type="entry name" value="FTR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006414">
        <property type="entry name" value="Ftr_formyl_trnsf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55112">
        <property type="entry name" value="SSF55112"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03119">
        <property type="entry name" value="one_C_fhcD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000138119" description="Formylmethanofuran--tetrahydromethanopterin formyltransferase">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00579"/>
        </source>
    </evidence>
    <sequence length="301" mass="32445" checksum="28207E48812B0296" modified="1996-11-01" version="1">MEINGVYIEDTFAEAFPIWVSRVLITAATKKWAKIAATEATGFGCSVIMCPAEAGIEKYVPPSKTPDGRPGFIIQICHPKKSELEHQMLERLGQCVLTCPTTAIFDAMGDMADEQLKVGFKLKFFGDGYEKKDELYGRKVYKIPIMGGEFITEAKFGIKKGVAGGNFFIMADTNASALIAAEAAVNAIASVDGVITPFPGGVVASGSKVGASNPKYKFMVATTNHKMCPTLKGVVEDSEIPEDVNGVYEIVIDGVDEESVKEAMKQGILAATRVKGVKKITAGNYGGKLGKYQFNLRELFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q57771</accession>
    <name>Y325_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0325</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0325</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64340">
        <property type="entry name" value="E64340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869822.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57771"/>
    <dbReference type="SMR" id="Q57771"/>
    <dbReference type="STRING" id="243232.MJ_0325"/>
    <dbReference type="EnsemblBacteria" id="AAB98313">
        <property type="protein sequence ID" value="AAB98313"/>
        <property type="gene ID" value="MJ_0325"/>
    </dbReference>
    <dbReference type="GeneID" id="1451179"/>
    <dbReference type="KEGG" id="mja:MJ_0325"/>
    <dbReference type="eggNOG" id="arCOG01564">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077867_0_0_2"/>
    <dbReference type="InParanoid" id="Q57771"/>
    <dbReference type="OMA" id="MMDYALV"/>
    <dbReference type="OrthoDB" id="76962at2157"/>
    <dbReference type="PhylomeDB" id="Q57771"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106794" description="Uncharacterized protein MJ0325">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <sequence length="308" mass="34597" checksum="7195B94307F3E60A" modified="1996-11-01" version="1">MEGLTVGLFGHVEGVGKELGKKGTSTDITLYNYKQGDKAVCYVEPTRYPDRINPLIYEINMMDYALVFIDEITGELGETLLALDMFGINNGAFVVGEYVDLDMLKNIISQTSMKDFEILERDFINIREKMINLNIERDYNGFVKIPIDHYFTVRSVGTVILGKVESGTVRVHDNLRVYPTDKMAMVRSIQIHDNDFKEAKAGNRVGLALKGITTDELDRGMILSNGELKVAKEIEININWNPFMQKTVKEGENYQIIVGLQSVSCVVEEVNKNKIKLSLQKEIAYDVGDKLCLIDGSAKIRILGVGKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q57782</accession>
    <name>Y336_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0336</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0336</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64341">
        <property type="entry name" value="H64341"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869834.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57782"/>
    <dbReference type="STRING" id="243232.MJ_0336"/>
    <dbReference type="EnsemblBacteria" id="AAB98324">
        <property type="protein sequence ID" value="AAB98324"/>
        <property type="gene ID" value="MJ_0336"/>
    </dbReference>
    <dbReference type="GeneID" id="1451192"/>
    <dbReference type="KEGG" id="mja:MJ_0336"/>
    <dbReference type="eggNOG" id="arCOG09641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056064_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106806" description="Uncharacterized protein MJ0336">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <sequence length="119" mass="12393" checksum="B27750A524DE2E5C" modified="1996-11-01" version="1">MTTENIISGIAIADLAPYRFGKITSTGIGFGTSTVSDDSISIDADGVIINSRAIYTAGMYVDLKVNGVQKVEIKEGESIAPGDYVIPSTDGSGKAVKSPIKAGFKVASVEGNYCTIILR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57787</accession>
    <name>Y341_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0341</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0341</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64342">
        <property type="entry name" value="E64342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869839.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57787"/>
    <dbReference type="SMR" id="Q57787"/>
    <dbReference type="STRING" id="243232.MJ_0341"/>
    <dbReference type="EnsemblBacteria" id="AAB98329">
        <property type="protein sequence ID" value="AAB98329"/>
        <property type="gene ID" value="MJ_0341"/>
    </dbReference>
    <dbReference type="GeneID" id="1451197"/>
    <dbReference type="KEGG" id="mja:MJ_0341"/>
    <dbReference type="eggNOG" id="arCOG09646">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044424_0_0_2"/>
    <dbReference type="OMA" id="TELIMFE"/>
    <dbReference type="OrthoDB" id="136072at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106811" description="Uncharacterized protein MJ0341">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <sequence length="120" mass="14055" checksum="495706AA47ED311F" modified="1996-11-01" version="1">MDKNEFLKKLDEKFKESEQKNLEALEKIRSNLPQLEIEIFGEKLTAIIPPLSVEKEMIEDAKNLEPLDFALKYIPILYGIPKEKVEELPSIVIAELIKKYFEAYKQLNKDKSFRNRVGTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57798</accession>
    <name>Y352_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0352</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0352</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64343">
        <property type="entry name" value="H64343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869851.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57798"/>
    <dbReference type="STRING" id="243232.MJ_0352"/>
    <dbReference type="PRIDE" id="Q57798"/>
    <dbReference type="EnsemblBacteria" id="AAB98341">
        <property type="protein sequence ID" value="AAB98341"/>
        <property type="gene ID" value="MJ_0352"/>
    </dbReference>
    <dbReference type="GeneID" id="1451209"/>
    <dbReference type="KEGG" id="mja:MJ_0352"/>
    <dbReference type="eggNOG" id="arCOG09665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149834_0_0_2"/>
    <dbReference type="OMA" id="MCKYELA"/>
    <dbReference type="OrthoDB" id="133427at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106823" description="Uncharacterized protein MJ0352">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <sequence length="239" mass="27547" checksum="98C05CE3E6595316" modified="1996-11-01" version="1">MCKYELAAKAHKNVYGVEIEVEEIKKQVEEIRKEHNNWIDESAAFVKWLETLELKDEFKKLREEERMNKNGNSIEVKASNNAMVVLEKTAEKVDSLDDVIEKMDSLDEDLMLLDEANEHLPLAYTYPDKKTGKEKIILSWAGIVKAMRMQGNIEVEPPTFQEVNGKIIATCRVRDLKRNIVMVGTAERVSPGRMGEEFKYTVLASKAIRNALKHIIEPKYLQMVIAEAKKRKSYVIITY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q57811</accession>
    <name>Y365_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0365</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0365</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64345">
        <property type="entry name" value="E64345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869864.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57811"/>
    <dbReference type="SMR" id="Q57811"/>
    <dbReference type="STRING" id="243232.MJ_0365"/>
    <dbReference type="EnsemblBacteria" id="AAB98356">
        <property type="protein sequence ID" value="AAB98356"/>
        <property type="gene ID" value="MJ_0365"/>
    </dbReference>
    <dbReference type="GeneID" id="1451222"/>
    <dbReference type="KEGG" id="mja:MJ_0365"/>
    <dbReference type="eggNOG" id="arCOG03130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521869_0_0_2"/>
    <dbReference type="OMA" id="PMEHRIV"/>
    <dbReference type="OrthoDB" id="139910at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038720">
        <property type="entry name" value="YprB_RNase_H-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13482">
        <property type="entry name" value="RNase_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106834" description="Uncharacterized protein MJ0365">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <sequence length="176" mass="20784" checksum="C62A30BD30321777" modified="1996-11-01" version="1">MAKAIIDIETTGLNPMEHRIVAIGVKLGDRDIILMDESEYYLLVNFWDTVEKEGIEKIIGFNIDFDWQFLKLRSLYHRLKIKHFRKYQGRVDLRQILNGSGGQYRKGTKLVDYCRFLGIDVPEDDANGSEIPELWEKFEEEGDEEAKRKICEHLKRDLERTWELYKILVDCGLIEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q57900</accession>
    <name>MFNE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">[5-(aminomethyl)furan-3-yl]methyl phosphate kinase</fullName>
            <ecNumber evidence="3">2.7.4.31</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">5-(aminomethyl)-3-furanmethanol phosphate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mfnE</name>
        <name evidence="4" type="synonym">adkB</name>
        <name type="ordered locus">MJ0458</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="Arch. Microbiol." volume="194" first="141" last="145">
            <title>A new class of adenylate kinase in methanogens is related to uridylate kinase.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Censky K."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="22002406"/>
            <dbReference type="DOI" id="10.1007/s00203-011-0759-9"/>
        </citation>
        <scope>FUNCTION AS AN ADENYLATE KINASE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2015" name="J. Bacteriol." volume="197" first="2850" last="2858">
            <title>Identification of the final two genes functioning in methanofuran biosynthesis in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Wang Y."/>
                <person name="Xu H."/>
                <person name="Jones M.K."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="26100040"/>
            <dbReference type="DOI" id="10.1128/JB.00401-15"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2013" name="Acta Crystallogr. F Struct. Biol. Commun." volume="69" first="1272" last="1274">
            <title>Crystallization and preliminary X-ray diffraction analysis of MJ0458, an adenylate kinase from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Wang X."/>
                <person name="Yuan Y."/>
                <person name="Teng M."/>
                <person name="Niu L."/>
                <person name="Gao Y."/>
            </authorList>
            <dbReference type="PubMed" id="24192367"/>
            <dbReference type="DOI" id="10.1107/S1744309113026638"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the formation of 5-(aminomethyl)-3-furanmethanol diphosphate (F1-PP) from 5-(aminomethyl)-3-furanmethanol phosphate (F1-P) and ATP (PubMed:26100040). In vitro, can also act as an adenylate kinase that catalyzes the transfer of a phosphoryl group from ATP to AMP, generating two molecules of ADP (PubMed:22002406).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>[5-(aminomethyl)-3-furyl]methyl phosphate + ATP = [5-(aminomethyl)furan-3-yl]methyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:47836"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83431"/>
            <dbReference type="ChEBI" id="CHEBI:88054"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.31"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Inhibited by EDTA.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">10.2 uM for ATP</KM>
            <KM evidence="2">26.2 uM for AMP</KM>
            <Vmax evidence="2">3.1 umol/min/mg enzyme for the adenylate kinase reaction (at 37 degrees Celsius)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 7.0.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MfnE family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.31" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64357">
        <property type="entry name" value="B64357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869957.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57900"/>
    <dbReference type="SMR" id="Q57900"/>
    <dbReference type="STRING" id="243232.MJ_0458"/>
    <dbReference type="EnsemblBacteria" id="AAB98446">
        <property type="protein sequence ID" value="AAB98446"/>
        <property type="gene ID" value="MJ_0458"/>
    </dbReference>
    <dbReference type="GeneID" id="1451319"/>
    <dbReference type="KEGG" id="mja:MJ_0458"/>
    <dbReference type="eggNOG" id="arCOG00859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089197_0_0_2"/>
    <dbReference type="InParanoid" id="Q57900"/>
    <dbReference type="OMA" id="LPHSWDV"/>
    <dbReference type="OrthoDB" id="85553at2157"/>
    <dbReference type="PhylomeDB" id="Q57900"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-19567"/>
    <dbReference type="BRENDA" id="2.7.4.3">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.7.4.31">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04240">
        <property type="entry name" value="AAK_UC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011375">
        <property type="entry name" value="MfnE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004857">
        <property type="entry name" value="Kin_aa_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106886" description="[5-(aminomethyl)furan-3-yl]methyl phosphate kinase">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="147"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9HLX1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22002406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="26100040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="22002406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="26100040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="26100040"/>
        </source>
    </evidence>
    <sequence length="216" mass="24080" checksum="37856E333F3D2A76" modified="1996-11-01" version="1">MHIVKIGGSLTYDAKPLLKALKNYAKENNKKIVIIPGGGEFANVVRKIDKALNISNSLSHKLAIKCMDLIGEVYAEIGYIKAYDTLFDLKREIEKEKIAILLPSKILLSTDIAEHSWAITSDSLSLYIGKLLDVREVIIATDVDGIYDKFPGGKLLNIINANDIKGLTSVDETFPILLKQFKMNAYVVNGRHPERVMDILEGKHNIYTKIVGIDKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57920</accession>
    <name>HJC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Holliday junction resolvase Hjc</fullName>
            <shortName evidence="2">Hjc</shortName>
            <ecNumber evidence="2">3.1.21.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">hjc</name>
        <name type="ordered locus">MJ0497</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are ligated to produce recombinant products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64362">
        <property type="entry name" value="A64362"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869998.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57920"/>
    <dbReference type="SMR" id="Q57920"/>
    <dbReference type="STRING" id="243232.MJ_0497"/>
    <dbReference type="EnsemblBacteria" id="AAB98490">
        <property type="protein sequence ID" value="AAB98490"/>
        <property type="gene ID" value="MJ_0497"/>
    </dbReference>
    <dbReference type="GeneID" id="1451359"/>
    <dbReference type="KEGG" id="mja:MJ_0497"/>
    <dbReference type="eggNOG" id="arCOG00919">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139546_2_0_2"/>
    <dbReference type="InParanoid" id="Q57920"/>
    <dbReference type="OMA" id="KNYALDY"/>
    <dbReference type="OrthoDB" id="97278at2157"/>
    <dbReference type="PhylomeDB" id="Q57920"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="archeal_HJR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="PTHR39651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106899" description="Holliday junction resolvase Hjc">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <sequence length="133" mass="15232" checksum="E49A8A128AF16506" modified="1996-11-01" version="1">MRHKYRKGSSFERELKRLLEKEGFAVIRSAGSKGVDLIAGRKGEVLIFECKTSSKTKFYINKEDIEKLISFSEIFGGKPYLAIKFNGEMLFINPFLLSTNGKNYVIDERIKAIAIDFYEVIGRGKQLKIDDLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q57952</accession>
    <name>GGR_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Digeranylgeranylglycerophospholipid reductase</fullName>
            <shortName evidence="1">DGGGPL reductase</shortName>
            <ecNumber evidence="1">1.3.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2,3-bis-O-geranylgeranylglyceryl phosphate reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Geranylgeranyl reductase</fullName>
            <shortName evidence="1">GGR</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0532</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is involved in the reduction of 2,3-digeranylgeranylglycerophospholipids (unsaturated archaeols) into 2,3-diphytanylglycerophospholipids (saturated archaeols) in the biosynthesis of archaeal membrane lipids. Catalyzes the formation of archaetidic acid (2,3-di-O-phytanyl-sn-glyceryl phosphate) from 2,3-di-O-geranylgeranylglyceryl phosphate (DGGGP) via the hydrogenation of each double bond of the isoprenoid chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>8 A + a 2,3-bis-O-phytanyl-sn-glycerol 1-phospholipid = a 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phospholipid + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64376"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:138139"/>
            <dbReference type="ChEBI" id="CHEBI:138140"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:64378"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(phytanyl)-sn-glycerol 1-phosphate + 8 A = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + 8 AH2</text>
            <dbReference type="Rhea" id="RHEA:64368"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:73125"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="1">
            <dbReference type="Rhea" id="RHEA:64370"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reduction reaction proceeds via syn addition of hydrogen for double bonds.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the geranylgeranyl reductase family. DGGGPL reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64366">
        <property type="entry name" value="D64366"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870036.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57952"/>
    <dbReference type="SMR" id="Q57952"/>
    <dbReference type="STRING" id="243232.MJ_0532"/>
    <dbReference type="EnsemblBacteria" id="AAB98523">
        <property type="protein sequence ID" value="AAB98523"/>
        <property type="gene ID" value="MJ_0532"/>
    </dbReference>
    <dbReference type="GeneID" id="1451397"/>
    <dbReference type="KEGG" id="mja:MJ_0532"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024648_0_0_2"/>
    <dbReference type="InParanoid" id="Q57952"/>
    <dbReference type="OMA" id="GMCRREV"/>
    <dbReference type="OrthoDB" id="31233at2157"/>
    <dbReference type="PhylomeDB" id="Q57952"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045550">
        <property type="term" value="F:geranylgeranyl reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046467">
        <property type="term" value="P:membrane lipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01287">
        <property type="entry name" value="DGGGPL_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023590">
        <property type="entry name" value="DGGGPL_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000005342" description="Digeranylgeranylglycerophospholipid reductase">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="14"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="206"/>
            <end position="212"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="287"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="291"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01287"/>
        </source>
    </evidence>
    <sequence length="391" mass="43094" checksum="A2F69AEEF8940EF2" modified="1996-11-01" version="1">MRELNDVYDVVVVGAGPGGSMASYASAKNGAKTLLIEKSQEIGEPVRCAEAIPSIEEFGLKPEPEFVRNIIKGGILFSPSGKKVTVTQDKAQGYVVERKIFDKYLAIRAAKAGAKVAVKTTAIGLERDGDYWNVIVEFLGEEYVIKTKVVIAADGVESNIAEYAGLKAKKKPLEICSCAEYEMTNVELLDKNMMEFYFGNEVAPGGYVWIFPKGETANVGLGVRDKKKKAIEYLEEFIENGLAKDRLKDATPIEFKVGGAPVSGPIEKTYTDGLLVVGDAAGQISPLTGGGIYLAMDCGLIAGEVASKAIKLNDWSEETLKEYERRWKEKHYEYLMSHLKYRKILEKMSDDELDALAEALGESLDGIDLKKFVKRIITKKPSLLKYFKDLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q57958</accession>
    <name>Y538_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0538</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0538</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64367">
        <property type="entry name" value="B64367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870042.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57958"/>
    <dbReference type="STRING" id="243232.MJ_0538"/>
    <dbReference type="EnsemblBacteria" id="AAB98536">
        <property type="protein sequence ID" value="AAB98536"/>
        <property type="gene ID" value="MJ_0538"/>
    </dbReference>
    <dbReference type="GeneID" id="1451403"/>
    <dbReference type="KEGG" id="mja:MJ_0538"/>
    <dbReference type="eggNOG" id="arCOG05037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1076106_0_0_2"/>
    <dbReference type="OMA" id="FCKPFDI"/>
    <dbReference type="OrthoDB" id="62876at2157"/>
    <dbReference type="PhylomeDB" id="Q57958"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106920" description="Uncharacterized protein MJ0538">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <sequence length="260" mass="30184" checksum="AD68C8FC3846EB3D" modified="1996-11-01" version="1">MIKIHALEEVKGNSKEIVEKEFENLANELKEKYNAKLKYVDEDIEEDENLKFYTKIGEFEINFDNFKDYINFCLKYGADIEVIKPEKLKLTANEINEVLALVISAFKSFMDTYKIGFDVYVKEKKDIDVEGYKKGKYDEDEIADFEEEGFIRVKAVFEAIGKNENEVVKNLLISLDRDEIIINKIITKNFNENNENFNGLMAVDLLCNPFEMFEIAYKYLPVAISIQRDEIELSLADIQDIGNELSGAMFELSHAVIMRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q57959</accession>
    <name>SYK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Lysine--tRNA ligase</fullName>
            <ecNumber>6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Lysyl-tRNA synthetase</fullName>
            <shortName>LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">lysS</name>
        <name type="ordered locus">MJ0539</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870043.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57959"/>
    <dbReference type="SMR" id="Q57959"/>
    <dbReference type="STRING" id="243232.MJ_0539"/>
    <dbReference type="PRIDE" id="Q57959"/>
    <dbReference type="EnsemblBacteria" id="AAB98532">
        <property type="protein sequence ID" value="AAB98532"/>
        <property type="gene ID" value="MJ_0539"/>
    </dbReference>
    <dbReference type="GeneID" id="1451404"/>
    <dbReference type="KEGG" id="mja:MJ_0539"/>
    <dbReference type="eggNOG" id="arCOG00485">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025562_1_0_2"/>
    <dbReference type="InParanoid" id="Q57959"/>
    <dbReference type="OMA" id="DWPMRWA"/>
    <dbReference type="OrthoDB" id="9880at2157"/>
    <dbReference type="PhylomeDB" id="Q57959"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042078">
        <property type="entry name" value="Lys-tRNA-ligase_SC_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="PTHR37940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="SSF48163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152753" description="Lysine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="28"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="278"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="530" mass="61921" checksum="5811837C8A349E9C" modified="1998-07-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57974</accession>
    <name>Y554_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0554</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0554</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0129 and MJ0587.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64369">
        <property type="entry name" value="B64369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870058.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57974"/>
    <dbReference type="SMR" id="Q57974"/>
    <dbReference type="DNASU" id="1451419"/>
    <dbReference type="EnsemblBacteria" id="AAB98545">
        <property type="protein sequence ID" value="AAB98545"/>
        <property type="gene ID" value="MJ_0554"/>
    </dbReference>
    <dbReference type="GeneID" id="1451419"/>
    <dbReference type="KEGG" id="mja:MJ_0554"/>
    <dbReference type="HOGENOM" id="CLU_1718222_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q57974"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035582">
        <property type="entry name" value="DUF5418"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17439">
        <property type="entry name" value="DUF5418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106928" description="Uncharacterized protein MJ0554">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="152" mass="17434" checksum="4408E3CA5E03EB2F" modified="1997-11-01" version="1">MVNEHKAHASFMFKIINVFVSFGFNLILGILIYDIFFNIDENLVVACILIAMPIIAFLILILTGGVHKELTYLQIYDKYKLMCEFIREITISTITSELATIATMILYQLQNPIKTITFLLLLIAFLAFGLIFTKLLIDAYFITLKKLKSLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58028</accession>
    <name>Y611_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative zinc metalloprotease MJ0611</fullName>
            <ecNumber>3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0611</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64376">
        <property type="entry name" value="C64376"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58028"/>
    <dbReference type="STRING" id="243232.MJ_0611"/>
    <dbReference type="EnsemblBacteria" id="AAB98604">
        <property type="protein sequence ID" value="AAB98604"/>
        <property type="gene ID" value="MJ_0611"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0611"/>
    <dbReference type="eggNOG" id="arCOG00614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099718_0_0_2"/>
    <dbReference type="InParanoid" id="Q58028"/>
    <dbReference type="OMA" id="SEFRAWY"/>
    <dbReference type="PhylomeDB" id="Q58028"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="S2P-M50-like"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000106957" description="Putative zinc metalloprotease MJ0611">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="191" mass="21834" checksum="0F613A043DEF7DA9" modified="1996-11-01" version="1">MSIFRFSQREIIDLTISVLAIAFIFSYPNFSILVFIISLIAVGSGFIFHELMHRTVARKYGAWSEFRAWYEGLILGFILKLVFGATFIAPGAVYIYKDYLTPEENGKIALAGPLTNVALAFVFFILMLIFKPGSLLYWIGIFGFHINLFLAGFNMLPIPPFDGEKVLKWNPFIWAIVGLPLIGYMLYMMFW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58037</accession>
    <name>MPTN_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin:alpha-L-glutamate ligase</fullName>
            <ecNumber>6.3.2.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>H(4)MPT:alpha-L-glutamate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mptN</name>
        <name type="ordered locus">MJ0620</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Proc. Natl. Acad. Sci. U.S.A." volume="100" first="9785" last="9790">
            <title>Glutathione synthetase homologs encode alpha-L-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses.</title>
            <authorList>
                <person name="Li H."/>
                <person name="Xu H."/>
                <person name="Graham D.E."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="12909715"/>
            <dbReference type="DOI" id="10.1073/pnas.1733391100"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP or GTP-dependent addition of one L-glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6,7,8-tetrahydromethanopterin + ATP + L-glutamate = 5,6,7,8-tetrahydrosarcinapterin + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:30567"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:59924"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; 5,6,7,8-tetrahydrosarcinapterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RimK family. MptN subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.33"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64377">
        <property type="entry name" value="D64377"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870125.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58037"/>
    <dbReference type="SMR" id="Q58037"/>
    <dbReference type="STRING" id="243232.MJ_0620"/>
    <dbReference type="EnsemblBacteria" id="AAB98615">
        <property type="protein sequence ID" value="AAB98615"/>
        <property type="gene ID" value="MJ_0620"/>
    </dbReference>
    <dbReference type="GeneID" id="1451486"/>
    <dbReference type="KEGG" id="mja:MJ_0620"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054353_2_0_2"/>
    <dbReference type="InParanoid" id="Q58037"/>
    <dbReference type="OMA" id="CYMNIAS"/>
    <dbReference type="OrthoDB" id="42812at2157"/>
    <dbReference type="PhylomeDB" id="Q58037"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-15293"/>
    <dbReference type="BRENDA" id="6.3.2.33">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00069"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="RpS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000205502" description="Tetrahydromethanopterin:alpha-L-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="101"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12909715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="291" mass="33277" checksum="2B6A3438290A3B0E" modified="1996-11-01" version="1">MKLGIITIERDAVVNDLIKSCEKYEVDYKVITPSNIVAGFNLDFKLKYYKSFLDELDCCFVRNLGWDSFFRFDVLKYLNHYIPVINPPDGIDRASNKFLTSVFLELNNLPQPKTVVTESINEAIVWIDKFEEAVLKPIFGCGGEGIVRVKKELPISTKLKILNEFKEKYNTFYIQEFIKPVRNEHRDIRAFVVDDEVVAAMYRIGGENWKNNVSQGGRVEKCEITEEIEKLALKAKNALGLFYAGVDLIESEDGLKVLEVNSTPSWIGLSKVSEVNIADKLLEKIIQYVKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58042</accession>
    <name>Y625_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0625</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0625</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64378">
        <property type="entry name" value="A64378"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870130.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58042"/>
    <dbReference type="SMR" id="Q58042"/>
    <dbReference type="STRING" id="243232.MJ_0625"/>
    <dbReference type="EnsemblBacteria" id="AAB98625">
        <property type="protein sequence ID" value="AAB98625"/>
        <property type="gene ID" value="MJ_0625"/>
    </dbReference>
    <dbReference type="GeneID" id="1451491"/>
    <dbReference type="KEGG" id="mja:MJ_0625"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q58042"/>
    <dbReference type="OMA" id="WYAIREI"/>
    <dbReference type="OrthoDB" id="132808at2157"/>
    <dbReference type="PhylomeDB" id="Q58042"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184669" description="Uncharacterized ATP-binding protein MJ0625">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="336" mass="40111" checksum="7B17DB2D17C412B6" modified="1997-11-01" version="1">MKFYNREKELNYLKTYCQLDPNSILFVYGPKSSGKSTVMRRVIKELEDSNIVFFYYNLRKYATYSKEEFLRVFFEKSEKKYLLNKLELNLGVFKFGVEENFDFNNLKLNDVFAKINESINAVVEDGKKPVLIIDELQKLKNIYFNGGKSLLNELFNLFVSLTKMEHLCHVICLTSDTLFIEEIYQSSTLENTSKYYLIDWLRKGTIRNILKEEGFSEEEINYCLDYLSLPYEIVDLIENKKLGLSVEETIRQWINIEKDKIKYLIDTTDLDEEELYKVLSKFKDKIKISYEKEVKKEEMKYFKFLIKNEILFYDVINGIIKPTSVKKWYAIREILK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58044</accession>
    <name>Y627_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0627</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0627</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64378">
        <property type="entry name" value="C64378"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870132.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58044"/>
    <dbReference type="STRING" id="243232.MJ_0627"/>
    <dbReference type="EnsemblBacteria" id="AAB98626">
        <property type="protein sequence ID" value="AAB98626"/>
        <property type="gene ID" value="MJ_0627"/>
    </dbReference>
    <dbReference type="GeneID" id="1451493"/>
    <dbReference type="KEGG" id="mja:MJ_0627"/>
    <dbReference type="eggNOG" id="arCOG12719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079448_0_0_2"/>
    <dbReference type="OMA" id="IFKISEQ"/>
    <dbReference type="OrthoDB" id="136114at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106961" description="Uncharacterized protein MJ0627">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <sequence length="117" mass="13866" checksum="AECD137581B92706" modified="1996-11-01" version="1">MEIDVKSPDLIINTIRGNEKIYFDKSIFEESLDNKFEIIQYLMKILERFLKIYDNHIKEIKLLIDSENHPEPHLIVVIKFKNKENIFKISEQIENKIYENPKSKNVLVYPITGGTDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58051</accession>
    <name>Y634_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized KamA family protein MJ0634</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0634</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the radical SAM superfamily. KamA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64379">
        <property type="entry name" value="B64379"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870139.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58051"/>
    <dbReference type="SMR" id="Q58051"/>
    <dbReference type="STRING" id="243232.MJ_0634"/>
    <dbReference type="EnsemblBacteria" id="AAB98629">
        <property type="protein sequence ID" value="AAB98629"/>
        <property type="gene ID" value="MJ_0634"/>
    </dbReference>
    <dbReference type="GeneID" id="1451500"/>
    <dbReference type="KEGG" id="mja:MJ_0634"/>
    <dbReference type="eggNOG" id="arCOG03246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_440512_0_0_2"/>
    <dbReference type="InParanoid" id="Q58051"/>
    <dbReference type="OMA" id="CQRMYDF"/>
    <dbReference type="OrthoDB" id="10285at2157"/>
    <dbReference type="PhylomeDB" id="Q58051"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="PTHR30538"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01070">
        <property type="entry name" value="PLP-dependent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00238">
        <property type="entry name" value="TIGR00238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000172295" description="Uncharacterized KamA family protein MJ0634">
        <location>
            <begin position="1"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="324"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="620" mass="73372" checksum="4A5E12147E5F159C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q58065</accession>
    <name>NAOX_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative NADH oxidase</fullName>
            <shortName>NOXase</shortName>
            <ecNumber>7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0649</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the four-electron reduction of molecular oxygen to water.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98641" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="7.1.1.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98641.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64381">
        <property type="entry name" value="A64381"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496570.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58065"/>
    <dbReference type="SMR" id="Q58065"/>
    <dbReference type="STRING" id="243232.MJ_0649"/>
    <dbReference type="EnsemblBacteria" id="AAB98641">
        <property type="protein sequence ID" value="AAB98641"/>
        <property type="gene ID" value="MJ_0649"/>
    </dbReference>
    <dbReference type="GeneID" id="1451515"/>
    <dbReference type="KEGG" id="mja:MJ_0649"/>
    <dbReference type="eggNOG" id="arCOG01069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_1_3_2"/>
    <dbReference type="InParanoid" id="Q58065"/>
    <dbReference type="OMA" id="ACGIPYW"/>
    <dbReference type="OrthoDB" id="40511at2157"/>
    <dbReference type="PhylomeDB" id="Q58065"/>
    <dbReference type="BRENDA" id="1.6.3.3">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="SSF55424"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0558">Oxidation</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0676">Redox-active center</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <feature type="chain" id="PRO_0000184704" description="Putative NADH oxidase">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Redox-active">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="152"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="271"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine sulfenic acid (-SOH)" evidence="1">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="448" mass="48876" checksum="8790664DF98D9DCD" modified="2005-03-01" version="2">MRAIIIGSGAAGLTTASTIRKYNKDMEIVVITKEKEIAYSPCAIPYVIEGAIKSFDDIIMHTPEDYKRERNIDILTETTVIDVDSKNNKIKCVDKDGNEFEMNYDYLVLATGAEPFIPPIEGKDLDGVFKVRTIEDGRAILKYIEENGCKKVAVVGAGAIGLEMAYGLKCRGLDVLVVEMAPQVLPRFLDPDMAEIVQKYLEKEGIKVMLSKPLEKIVGKEKVEAVYVDGKLYDVDMVIMATGVRPNIELAKKAGCKIGKFAIEVNEKMQTSIPNIYAVGDCVEVIDFITGEKTLSPFGTAAVRQGKVAGKNIAGVEAKFYPVLNSAVSKIGDLEIGGTGLTAFSANLKRIPIVIGRAKALTRARYYPGGKEIEIKMIFNEDGKVVGCQIVGGERVAERIDAMSIAIFKKVSAEELANMEFCYAPPVSMVHEPLSLAAEDALKKLSNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q58081</accession>
    <name>AMPPA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AMP phosphorylase</fullName>
            <shortName evidence="1">AMPpase</shortName>
            <ecNumber evidence="1">2.4.2.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside monophosphate phosphorylase</fullName>
            <shortName evidence="1">NMP phosphorylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0667</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:36975"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CMP + phosphate = alpha-D-ribose 1,5-bisphosphate + cytosine</text>
            <dbReference type="Rhea" id="RHEA:36987"/>
            <dbReference type="ChEBI" id="CHEBI:16040"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>phosphate + UMP = alpha-D-ribose 1,5-bisphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:36991"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.57" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64383">
        <property type="entry name" value="C64383"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870172.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58081"/>
    <dbReference type="SMR" id="Q58081"/>
    <dbReference type="STRING" id="243232.MJ_0667"/>
    <dbReference type="EnsemblBacteria" id="AAB98662">
        <property type="protein sequence ID" value="AAB98662"/>
        <property type="gene ID" value="MJ_0667"/>
    </dbReference>
    <dbReference type="GeneID" id="1451533"/>
    <dbReference type="KEGG" id="mja:MJ_0667"/>
    <dbReference type="eggNOG" id="arCOG02013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025040_6_0_2"/>
    <dbReference type="InParanoid" id="Q58081"/>
    <dbReference type="OMA" id="DVWRRMI"/>
    <dbReference type="OrthoDB" id="43931at2157"/>
    <dbReference type="PhylomeDB" id="Q58081"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046125">
        <property type="term" value="P:pyrimidine deoxyribonucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02132">
        <property type="entry name" value="AMP_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017713">
        <property type="entry name" value="AMP_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017872">
        <property type="entry name" value="Pyrmidine_PPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013466">
        <property type="entry name" value="Thymidine/AMP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="PTHR10515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000478">
        <property type="entry name" value="TP_PyNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="SSF54680"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03327">
        <property type="entry name" value="AMP_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02645">
        <property type="entry name" value="ARCH_P_rylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00647">
        <property type="entry name" value="THYMID_PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000059088" description="AMP phosphorylase">
        <location>
            <begin position="1"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="194"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02132"/>
        </source>
    </evidence>
    <sequence length="503" mass="54722" checksum="EFBB97D4A9ADEE18" modified="1996-11-01" version="1">MLFLKVRVLDIDLENLVLINSEDLKSSQYFPQDRVVVEFKGKEVIGILHSSTTLINRGEIGLPQKVVKELGVKEGDIVTIKHAEKPKSLPYIRKKMDGNKLKKEEIFEIIDEMVDGKLTNIEISAFVTSLYINGMDMDEIEAMTIRMAETGEMVNWEGHIFDVHSIGGVPGNKYALLVVPIVASAGLKIPKTSSRAITSAAGTADVVEVLTRVDLTIEEIKRVVKETNGCMVWGGALDLAPADDITINVERPLGIDPEPLLLSSVMAKKLAMGVNKLLIDIPTGYGAKVKSIKEASSLARKFIELSDRLRIVTECAITYGGQPIGRAIGPALEAKEALLALEDYTQAPTSLVEKSISLAGILLEMGGVAPTGEGKELAEDLLARGKAHDKFMEIIVAQGGKEVSSDEIEVGKYKADIHSPIDGYVTRISNAGITKIAKEAGAPNDKKAGIYLNVKVGNKVEKGDVLYTIYSDSEERLKSAIKLARILYPIKVEGMLLQKISRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58089</accession>
    <name>Y676_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative hydrogenase expression/formation protein MJ0676</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0676</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64384">
        <property type="entry name" value="D64384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870181.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58089"/>
    <dbReference type="SMR" id="Q58089"/>
    <dbReference type="STRING" id="243232.MJ_0676"/>
    <dbReference type="EnsemblBacteria" id="AAB98671">
        <property type="protein sequence ID" value="AAB98671"/>
        <property type="gene ID" value="MJ_0676"/>
    </dbReference>
    <dbReference type="GeneID" id="1451542"/>
    <dbReference type="KEGG" id="mja:MJ_0676"/>
    <dbReference type="eggNOG" id="arCOG00636">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049733_0_0_2"/>
    <dbReference type="InParanoid" id="Q58089"/>
    <dbReference type="OMA" id="CGNGGKE"/>
    <dbReference type="OrthoDB" id="34102at2157"/>
    <dbReference type="PhylomeDB" id="Q58089"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046892">
        <property type="term" value="P:peptidyl-S-carbamoyl-L-cysteine dehydration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02197">
        <property type="entry name" value="HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="PTHR30303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02124">
        <property type="entry name" value="hypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000201462" description="Putative hydrogenase expression/formation protein MJ0676">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="335" mass="35813" checksum="1676B351C033A964" modified="1996-11-01" version="1">MKITRMHGAGGKVMQELIKDVILKNLEITSVNGGIGLESLDDSATIPIGDKEIVFTVDGHTVKPIFFPGGDIGRLAVSGTVNDLAVMGAKPLALSLSLIIPEGFNLEDLEKIVKSINETSKEAEVAIITGDTKVSDGVDDIIISTAGIGIVDRGKAIRDCNVQEGDAIIVSGNIGEHGLAILLSREGFDFETNIKSDVAPINKLIERVLEEGIQINAMKDPTRGGLADALNEMAEKSNIGITIFEDKIPISDEVQSICDILGLDPLTIANEGKVVMAVKKEDAERCLEILREHPLGKNAEIIGYATKEHKGVIIETIVGRRIVDMPIGDPIPRVC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58137</accession>
    <name>Y727_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0727</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0727</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64390">
        <property type="entry name" value="G64390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870232.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58137"/>
    <dbReference type="SMR" id="Q58137"/>
    <dbReference type="STRING" id="243232.MJ_0727"/>
    <dbReference type="EnsemblBacteria" id="AAB98723">
        <property type="protein sequence ID" value="AAB98723"/>
        <property type="gene ID" value="MJ_0727"/>
    </dbReference>
    <dbReference type="GeneID" id="1451604"/>
    <dbReference type="KEGG" id="mja:MJ_0727"/>
    <dbReference type="eggNOG" id="arCOG01549">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_932593_0_0_2"/>
    <dbReference type="OMA" id="CERCYYL"/>
    <dbReference type="OrthoDB" id="46044at2157"/>
    <dbReference type="PhylomeDB" id="Q58137"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107004" description="Uncharacterized protein MJ0727">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <sequence length="298" mass="33519" checksum="4DAAD534096A680D" modified="1996-11-01" version="1">MKIRGFESSMMGKDIDFIPPAMTRLCCLNEISHALAGVMAVEKAYNITVPNEGQYLREIARLGEIVEVDAIKLREFKNTDDLADIGNKIKSVLGKKAKYLAVGGVLENISDKRKEKLINLAKEGLNLVDKDFVKLVDERKAKIPLPDVELIDAYNFDANKVETNGLPKTALYDGKVVYSGSLARMYKEGLINSKNLWDVLSSRMIEIEFCLNKIIELLNKLKLTHPYMEPIIKDGKAIGEAVIEGGEGIVYHKVELLGREILDYTILTSENFNKAVLDSVDNDEAKRIIQLCERCYYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q58159</accession>
    <name>Y749_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0749</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0749</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64393">
        <property type="entry name" value="E64393"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58159"/>
    <dbReference type="STRING" id="243232.MJ_0749"/>
    <dbReference type="EnsemblBacteria" id="AAB98742">
        <property type="protein sequence ID" value="AAB98742"/>
        <property type="gene ID" value="MJ_0749"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0749"/>
    <dbReference type="eggNOG" id="arCOG02772">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033147_1_0_2"/>
    <dbReference type="InParanoid" id="Q58159"/>
    <dbReference type="OMA" id="TIFFIRA"/>
    <dbReference type="PhylomeDB" id="Q58159"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159311" description="Uncharacterized protein MJ0749">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="3">
        <location>
            <begin position="185"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="3">
        <location>
            <begin position="210"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="246" mass="28500" checksum="40B6D4918D395F0C" modified="1996-11-01" version="1">MDSRVYIIKFDNWGFMVNLMNKIQILRKISQTLFFVRALIVTGFYLSIVGFIKRFIIGDRILATIITKIIAIVLAFIAGRVFCGWMCPFGFLFNLVYELRVKLFKLKKLPTVDEKIHNKLIYFKYVVLILVVLAYLSGVKISGYTLAYLLLALFLVLGFIYPMFFCRYVCPVGSLLSIFARFSIFKLKLDENKCVGCRLCERKCPMQIKITEKIDQMECIRCFECMSVCKKGALSFSAFTKNTKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58166</accession>
    <name>Y756_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0756</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0756</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64394">
        <property type="entry name" value="D64394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870261.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58166"/>
    <dbReference type="STRING" id="243232.MJ_0756"/>
    <dbReference type="EnsemblBacteria" id="AAB98757">
        <property type="protein sequence ID" value="AAB98757"/>
        <property type="gene ID" value="MJ_0756"/>
    </dbReference>
    <dbReference type="GeneID" id="1451633"/>
    <dbReference type="KEGG" id="mja:MJ_0756"/>
    <dbReference type="eggNOG" id="arCOG07800">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870748_0_0_2"/>
    <dbReference type="OMA" id="PPWMAYQ"/>
    <dbReference type="OrthoDB" id="105619at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013996" description="Uncharacterized protein MJ0756">
        <location>
            <begin position="20"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="136" mass="15370" checksum="A501A6B7D97F2BD0" modified="1996-11-01" version="1" precursor="true">MKKLLMVILGIALIGMAYAFPPWMAYQTQTTENTDINPVDILKTAEVVQHTTPFGYNLSHLEIDGKIVGVLWKDVDLSKLEVGEPFNTPFGEKYPLYYDRELVGFIFTNHPASHYGYGMRGGYGCHCHCGCCCWQQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58170</accession>
    <name>Y760_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0760</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0760</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64394">
        <property type="entry name" value="H64394"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58170"/>
    <dbReference type="SMR" id="Q58170"/>
    <dbReference type="STRING" id="243232.MJ_0760"/>
    <dbReference type="DNASU" id="1451637"/>
    <dbReference type="EnsemblBacteria" id="AAB98751">
        <property type="protein sequence ID" value="AAB98751"/>
        <property type="gene ID" value="MJ_0760"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0760"/>
    <dbReference type="eggNOG" id="arCOG02066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088479_0_0_2"/>
    <dbReference type="OMA" id="KPTIFLM"/>
    <dbReference type="PhylomeDB" id="Q58170"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SSF64307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107019" description="Uncharacterized protein MJ0760">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <sequence length="275" mass="30542" checksum="7E81636BD7CAEFB3" modified="1996-11-01" version="1">MIRMIRKFKVKGLRSPSLLIDMILNDTEEGILVVETDGEEQIKDIEKLLKKYNLKYEVDGNVVKIYVGEIKADKTINVVGATCPGPIMMVSDMLSKMKNGEILEIICGKNSLTDLTEGLKGMGNEIIKVEDKGDGTYRILVKKGEKKEEKAAVTKIDELFIINTTGTGNAEKAYATFMMADVALKMNLKPTIFLMMDGASLALKGECDRVKHPAFPKLGDLVRDILSKGVKIYVCELSAEFRGINEKNLEEGFEIAGAPTFLNYLSKPNVRPVWL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58207</accession>
    <name>Y797_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter permease MJ0797</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0797</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64399">
        <property type="entry name" value="E64399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870307.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58207"/>
    <dbReference type="SMR" id="Q58207"/>
    <dbReference type="STRING" id="243232.MJ_0797"/>
    <dbReference type="TCDB" id="3.A.1.122.14">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98792">
        <property type="protein sequence ID" value="AAB98792"/>
        <property type="gene ID" value="MJ_0797"/>
    </dbReference>
    <dbReference type="GeneID" id="1451679"/>
    <dbReference type="KEGG" id="mja:MJ_0797"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="InParanoid" id="Q58207"/>
    <dbReference type="OMA" id="GSLYRIR"/>
    <dbReference type="OrthoDB" id="45576at2157"/>
    <dbReference type="PhylomeDB" id="Q58207"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000107049" description="Uncharacterized ABC transporter permease MJ0797">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="329"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="367" mass="41039" checksum="3CC882FF21ED3746" modified="1997-11-01" version="1">MYFELAKRNLKRNLLRSILALLGIIIGVAAISSLGILGGGLKQGIMENLGSISNYIIVFPNYQNGYTSFDKRDIEKLRVLNCKVIPVYATSDFVYIKGKNRKAYANIFGIDKNDIKYLNLKVKVSDTSVAVDTFFSNVNDVNVGNQLEIKNISLRICGIYNSTFLFPDNSLILTAKTYRRFYGENNYNYSRIILYVKNINDIDKIKNETDKILNRKEKKCIIISLNSILEAINGVITKVSYFLMGIGAISLLVAGIGIGNVMLMSVVERTTEIGVMRSIGASKKDIIILFLYEALILGVIGSLIGAFLSLFFGYLIVHYLLKTSLSYYAIFYMIIGIIFGILTSLISALYPAYKASKLDPIKSLRNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58217</accession>
    <name>Y807_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative lyase MJ0807</fullName>
            <ecNumber>4.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0807</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the chorismate pyruvate-lyase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64400">
        <property type="entry name" value="G64400"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58217"/>
    <dbReference type="SMR" id="Q58217"/>
    <dbReference type="STRING" id="243232.MJ_0807"/>
    <dbReference type="EnsemblBacteria" id="AAB98807">
        <property type="protein sequence ID" value="AAB98807"/>
        <property type="gene ID" value="MJ_0807"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0807"/>
    <dbReference type="eggNOG" id="arCOG01031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107938_2_0_2"/>
    <dbReference type="InParanoid" id="Q58217"/>
    <dbReference type="OMA" id="RTYNIIH"/>
    <dbReference type="PhylomeDB" id="Q58217"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-18799"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002800">
        <property type="entry name" value="Rv2949c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01947">
        <property type="entry name" value="DUF98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="SSF64288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107056" description="Putative lyase MJ0807">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="184" mass="21548" checksum="086471223FBE61D6" modified="1996-11-01" version="1">MQKTKLRLIFMIIYKEIAKLNKTFPLLNEEKILLGTDGSVTNILEILFEGECRVETINQKIVANTNYREVILKVNNIPLVYAVSKTPFKNIEEENLREEIKRDLLSADIPIGKIIRKHNLETRREIKYIGIAEIDDYLKSLLKTNYSRLPKRTYNIIYKNKVLMEITEIFAVRGKLVKNTYSID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58218</accession>
    <name>Y808_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0808</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0808</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64400">
        <property type="entry name" value="H64400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870318.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58218"/>
    <dbReference type="SMR" id="Q58218"/>
    <dbReference type="STRING" id="243232.MJ_0808"/>
    <dbReference type="PRIDE" id="Q58218"/>
    <dbReference type="DNASU" id="1451690"/>
    <dbReference type="EnsemblBacteria" id="AAB98808">
        <property type="protein sequence ID" value="AAB98808"/>
        <property type="gene ID" value="MJ_0808"/>
    </dbReference>
    <dbReference type="GeneID" id="1451690"/>
    <dbReference type="KEGG" id="mja:MJ_0808"/>
    <dbReference type="eggNOG" id="arCOG00946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044176_1_0_2"/>
    <dbReference type="InParanoid" id="Q58218"/>
    <dbReference type="OMA" id="YHFYPGS"/>
    <dbReference type="OrthoDB" id="39920at2157"/>
    <dbReference type="PhylomeDB" id="Q58218"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PTHR30352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000107057" description="Uncharacterized protein MJ0808">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="67"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="333" mass="38337" checksum="C31C2477F6050893" modified="1996-11-01" version="1">MREAMFYEKLDDNKVRCHICPRHCIIKEGERGFCWNRENINGVLYAVGYGKVCSLAIDPIEKKPLFHFYPTTQVVSLAIGGCNFRCLHCQNWTISQFPPDEIPYREMTPEEIVEVAIRYNCPGISYTYTEPTVYYEFMYDTSVIARENGMFNVMITNGYIEKEPLKALPVDAMNIDIKGNADFYKKVCKATLEPVLETCKLAKKLGIWVEVTNLIVPNYNDNIDDLLFIIHFVRDELGRETPLHFSRFHPDYKLTDVPPTPIETLEMARNLAIEEGLKYVYIGNVPGHEGENTYCPNCGALLIERYIFNAKIINLDVETKRCKICGEKIDIVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58220</accession>
    <name>Y810_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein MJ0810</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0810</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98809" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98809.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64401">
        <property type="entry name" value="B64401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496635.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58220"/>
    <dbReference type="SMR" id="Q58220"/>
    <dbReference type="STRING" id="243232.MJ_0810"/>
    <dbReference type="EnsemblBacteria" id="AAB98809">
        <property type="protein sequence ID" value="AAB98809"/>
        <property type="gene ID" value="MJ_0810"/>
    </dbReference>
    <dbReference type="GeneID" id="1451693"/>
    <dbReference type="KEGG" id="mja:MJ_0810"/>
    <dbReference type="eggNOG" id="arCOG01336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095686_1_1_2"/>
    <dbReference type="InParanoid" id="Q58220"/>
    <dbReference type="OMA" id="RGCIGFP"/>
    <dbReference type="OrthoDB" id="107157at2157"/>
    <dbReference type="PhylomeDB" id="Q58220"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="PTHR13016"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="SSF143447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00296">
        <property type="entry name" value="TIGR00296"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142377" description="Protein MJ0810">
        <location>
            <begin position="1"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="AMMECR1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="201" mass="22936" checksum="E5C68B8FE4609B76" modified="2003-05-30" version="2">MRLLTLEEGTFAVRYARAVIENYLAGKKIVIESYPEVFNEKRGCFCTLHTYPDKELRGCIGIPEPIMPLIEALEEAAISAATKDPRFPPVTLEEMDSIVVEVSILTPPELIKVNHPKEYLEKIKIGRDGLIIEYGFYRGLLLPQVPVEYGWDVEEYLAHLCLKAGLPPDMWLAEGVKIYRFEAQIFEEVEPRGEVIEKKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58223</accession>
    <name>CBIH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable cobalt-factor III C(17)-methyltransferase</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cobalt-precorrin-3 methyltransferase</fullName>
            <shortName>Cobalt-precorrin-3 methylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cbiH</name>
        <name type="synonym">cobJ</name>
        <name type="ordered locus">MJ0813</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methyltransferase that likely catalyzes the ring contraction and methylation of C-17 in cobalt-factor III to form cobalt-factor IV. May also convert cobalt-precorrin-3 to cobalt-precorrin-4.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Co(II)-factor III + H(+) + S-adenosyl-L-methionine = Co(II)-factor IV + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:45852"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73299"/>
            <dbReference type="ChEBI" id="CHEBI:85471"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 3/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64401">
        <property type="entry name" value="E64401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870324.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58223"/>
    <dbReference type="SMR" id="Q58223"/>
    <dbReference type="STRING" id="243232.MJ_0813"/>
    <dbReference type="EnsemblBacteria" id="AAB98812">
        <property type="protein sequence ID" value="AAB98812"/>
        <property type="gene ID" value="MJ_0813"/>
    </dbReference>
    <dbReference type="GeneID" id="1451696"/>
    <dbReference type="KEGG" id="mja:MJ_0813"/>
    <dbReference type="eggNOG" id="arCOG00647">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047948_2_0_2"/>
    <dbReference type="InParanoid" id="Q58223"/>
    <dbReference type="OMA" id="VWDDRMV"/>
    <dbReference type="OrthoDB" id="49464at2157"/>
    <dbReference type="PhylomeDB" id="Q58223"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00225"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150403" description="Probable cobalt-factor III C(17)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="249" mass="28131" checksum="EE25D9F73D0C0911" modified="1996-11-01" version="1">MLYVVGIGSGNERHFTKEAEEILNKVDLIVCYKNYKKFVERLNKPIYTTGMTREIDRVDYALKEAKDKDVALVSSGDATIYGLASLAYEINAVKGYNVDIKVVPGITACSLASAILGSPLNHDFVVISFSDLLTPLETILKRFRCALEGDFVICIYNPLSKRRKEPFLKAMEILAEFAKDKDYIIGIVKNAGRNKEEVVITNFKDLYKNLEKYLEFIDMNTILIIGNSSTKIINGKMITPRGYLDKYKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58246</accession>
    <name>Y836_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0836</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0836</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64404">
        <property type="entry name" value="D64404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870350.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58246"/>
    <dbReference type="SMR" id="Q58246"/>
    <dbReference type="STRING" id="243232.MJ_0836"/>
    <dbReference type="EnsemblBacteria" id="AAB98839">
        <property type="protein sequence ID" value="AAB98839"/>
        <property type="gene ID" value="MJ_0836"/>
    </dbReference>
    <dbReference type="GeneID" id="1451722"/>
    <dbReference type="KEGG" id="mja:MJ_0836"/>
    <dbReference type="eggNOG" id="arCOG05056">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1998728_0_0_2"/>
    <dbReference type="OMA" id="LWCYCPF"/>
    <dbReference type="OrthoDB" id="94899at2157"/>
    <dbReference type="PhylomeDB" id="Q58246"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007212">
        <property type="entry name" value="Zf-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04071">
        <property type="entry name" value="zf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107073" description="Uncharacterized protein MJ0836">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <sequence length="119" mass="14176" checksum="196AB7DB9B52BC83" modified="1996-11-01" version="1">MIELAKNHLKKVLEVCGANRNCEYYPCHFDGQVCLWCYCPFYPCEDEELGEYVEKKDGTKIWSCMKCFWVHREDVATEILREILNLTKDKDIDEALKLLDNHELMLKIKDRVKAKYPNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58271</accession>
    <name>RNJ_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease J</fullName>
            <shortName evidence="1">RNase J</shortName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnj</name>
        <name type="ordered locus">MJ0861</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">An RNase that has 5'-3' exonuclease activity. May be involved in RNA degradation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64407">
        <property type="entry name" value="E64407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870376.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58271"/>
    <dbReference type="SMR" id="Q58271"/>
    <dbReference type="STRING" id="243232.MJ_0861"/>
    <dbReference type="EnsemblBacteria" id="AAB98866">
        <property type="protein sequence ID" value="AAB98866"/>
        <property type="gene ID" value="MJ_0861"/>
    </dbReference>
    <dbReference type="GeneID" id="1451750"/>
    <dbReference type="KEGG" id="mja:MJ_0861"/>
    <dbReference type="eggNOG" id="arCOG00546">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008727_4_1_2"/>
    <dbReference type="InParanoid" id="Q58271"/>
    <dbReference type="OMA" id="KNTCVFE"/>
    <dbReference type="OrthoDB" id="12384at2157"/>
    <dbReference type="PhylomeDB" id="Q58271"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01492">
        <property type="entry name" value="RNase_J_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004613">
        <property type="entry name" value="RNase_J"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042173">
        <property type="entry name" value="RNase_J_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030879">
        <property type="entry name" value="RNase_J_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001587">
        <property type="entry name" value="RNase_J_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00649">
        <property type="entry name" value="MG423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01292">
        <property type="entry name" value="UPF0036"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000215275" description="Ribonuclease J">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="383"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01492"/>
        </source>
    </evidence>
    <sequence length="448" mass="50062" checksum="FEF70CFCEF78770D" modified="1996-11-01" version="1">MKLEIIAIGGYEEVGRNMTAVNVDGEIIILDMGIRLDRVLIHEDTDISKLHSLELIEKGIIPNDTVMKNIEGEVKAIVLSHGHLDHIGAVPKLAHRYNAPIIGTPYTIELVKREILSEKKFDVRNPLIVLNAGESIDLTPNITLEFIRITHSIPDSVLPVLHTPYGSIVYGNDFKFDNFPVVGERPDYRAIKKVGKNGVLCFISETTRINHEGKTPPEIIASGLLKNDLLAADNDKHGIIVTTFSSHIARIKSITDIAEKMGRTPVLLGRSMMRFCGIAQDIGLVKFPEDLRIYGDPSSIEMALKNIVKEGKEKYLIIATGHQGEEGAVLSRMATNKTPYKFEKYDCVVFSADPIPNPMNAAQRYMLESRLKLLGVRIFKGAHVSGHAAKEDHRDMLRWLNPEHIIPSHGDFNLTAEYTKLAEEEGYRLGEDVHLLRNGQCLSFERII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58273</accession>
    <name>HDRB2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CoB--CoM heterodisulfide reductase subunit B 2</fullName>
            <ecNumber evidence="2">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrB2</name>
        <name type="ordered locus">MJ0863</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HdrB family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64407">
        <property type="entry name" value="G64407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870378.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58273"/>
    <dbReference type="SMR" id="Q58273"/>
    <dbReference type="STRING" id="243232.MJ_0863"/>
    <dbReference type="EnsemblBacteria" id="AAB98868">
        <property type="protein sequence ID" value="AAB98868"/>
        <property type="gene ID" value="MJ_0863"/>
    </dbReference>
    <dbReference type="GeneID" id="1451752"/>
    <dbReference type="KEGG" id="mja:MJ_0863"/>
    <dbReference type="eggNOG" id="arCOG00338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="InParanoid" id="Q58273"/>
    <dbReference type="OMA" id="YMPGASC"/>
    <dbReference type="OrthoDB" id="73750at2157"/>
    <dbReference type="PhylomeDB" id="Q58273"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017678">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03288">
        <property type="entry name" value="CoB_CoM_SS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150066" description="CoB--CoM heterodisulfide reductase subunit B 2">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LY38"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="295" mass="32789" checksum="35A2A278491E8ED0" modified="1996-11-01" version="1">MKYAFFLGCIMPHRYPGVEKATKIVMEELGVELEYMPGASCCPAPGVFGSFDQKTWLTLAARNLCIAEEMGLDIVTVCNGCYGSLFEAAHILHENKEALDFVNEKLDKIGKQYKGTIKVRHFAELIYKDIGVDKIKEKVVKPLDVLNVAIHYGCHFLKPSDVKHLDSPERPKLLEEIVAATGAKPVMYRDYLMCCGAGGGVRARFLPTALDMTKEKIRNMLEAGADCTVNVCPFCHLQFDRGQVEIKEKFGEEYKLPVLHLSQLLGLAFGMKPEDLAVSVHAIPVDPVLKKLGIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58294</accession>
    <name>RFCL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Replication factor C large subunit</fullName>
            <shortName>RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">rfcL</name>
        <name type="ordered locus">MJ0884</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Acta Crystallogr. D" volume="58" first="519" last="521">
            <title>Cloning, purification, crystallization and preliminary X-ray studies of RFC boxes II-VIII of replication factor C from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Lee I."/>
                <person name="Lokanath N.K."/>
                <person name="Min K."/>
                <person name="Ha S.C."/>
                <person name="Kim D.Y."/>
                <person name="Kim K.K."/>
            </authorList>
            <dbReference type="PubMed" id="11856841"/>
            <dbReference type="DOI" id="10.1107/S090744490102159X"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64410">
        <property type="entry name" value="D64410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870398.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58294"/>
    <dbReference type="SMR" id="Q58294"/>
    <dbReference type="STRING" id="243232.MJ_0884"/>
    <dbReference type="PRIDE" id="Q58294"/>
    <dbReference type="EnsemblBacteria" id="AAB98888">
        <property type="protein sequence ID" value="AAB98888"/>
        <property type="gene ID" value="MJ_0884"/>
    </dbReference>
    <dbReference type="GeneID" id="1451773"/>
    <dbReference type="KEGG" id="mja:MJ_0884"/>
    <dbReference type="eggNOG" id="arCOG00470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027255_1_1_2"/>
    <dbReference type="InParanoid" id="Q58294"/>
    <dbReference type="OMA" id="GGWTRYG"/>
    <dbReference type="OrthoDB" id="24257at2157"/>
    <dbReference type="PhylomeDB" id="Q58294"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135952" description="Replication factor C large subunit">
        <location>
            <begin position="1"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="474"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="423"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="474"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="516" mass="58770" checksum="800BF6A70560859E" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58319</accession>
    <name>RELE2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative toxin RelE2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">relE2</name>
        <name type="ordered locus">MJ0909</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. Its cognate antitoxin is RelB2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelE toxin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64413">
        <property type="entry name" value="E64413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870423.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58319"/>
    <dbReference type="SMR" id="Q58319"/>
    <dbReference type="STRING" id="243232.MJ_0909"/>
    <dbReference type="EnsemblBacteria" id="AAB98911">
        <property type="protein sequence ID" value="AAB98911"/>
        <property type="gene ID" value="MJ_0909"/>
    </dbReference>
    <dbReference type="GeneID" id="1451798"/>
    <dbReference type="KEGG" id="mja:MJ_0909"/>
    <dbReference type="eggNOG" id="arCOG01663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155761_5_1_2"/>
    <dbReference type="OMA" id="YRIGFKY"/>
    <dbReference type="OrthoDB" id="125851at2157"/>
    <dbReference type="PhylomeDB" id="Q58319"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02385">
        <property type="entry name" value="RelE_StbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107099" description="Putative toxin RelE2">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="91" mass="11146" checksum="4E42600B3466CD72" modified="1996-11-01" version="1">MKQWKYLLKKSFIKDLKELPKNIQEKIKKLVFEEIPNKNNPPEIPNVKKLKGADSYYRIRVGDYRIGFKYENGKIVFYRVLHRKQIYKRFP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q58346</accession>
    <name>P936_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphodiesterase MJ0936</fullName>
            <ecNumber>3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0936</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference evidence="3 4 5" key="2">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="31854" last="31862">
            <title>Structural and functional characterization of a novel phosphodiesterase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Chen S."/>
                <person name="Yakunin A.F."/>
                <person name="Kuznetsova E."/>
                <person name="Busso D."/>
                <person name="Pufan R."/>
                <person name="Proudfoot M."/>
                <person name="Kim R."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="15128743"/>
            <dbReference type="DOI" id="10.1074/jbc.M401059200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-165 OF NATIVE PROTEIN AND IN COMPLEXES WITH NICKEL AND MANGANESE</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Shows phosphodiesterase activity, hydrolyzing phosphodiesters bonds in the artificial chromogenic substrates bis-p-nitrophenyl phosphate (bis-pNPP), and less efficiently thymidine 5'-monophosphate p-nitrophenyl ester (pNP-TMP) and p-nitrophenylphosphorylcholine (pNPPC). No catalytic activity was found toward cAMP or cGMP, nucleotides or phospholipase substrates such as phosphatidylcholine. The physiological substrate is unknown.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal ions per subunit. Most effective are nickel and manganese.</text>
    </comment>
    <comment type="activity regulation">
        <text>Competitively inhibited by phosphate.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">0.15 mM for bis-pNPP (in the presence of Ni(2+))</KM>
            <KM evidence="1">0.035 mM for pNP-TMP (in the presence of Ni(2+))</KM>
            <KM evidence="1">3.33 mM for pNPPC (in the presence of Ni(2+))</KM>
            <KM evidence="1">1.53 mM for pNPPC (in the presence of Mn(2+))</KM>
        </kinetics>
        <phDependence>
            <text evidence="1">Optimum pH is 9.4-9.8.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64416">
        <property type="entry name" value="H64416"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870450.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1S3L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="1S3M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="1S3N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="2AHD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A/B/C/D=1-165"/>
    </dbReference>
    <dbReference type="PDBsum" id="1S3L"/>
    <dbReference type="PDBsum" id="1S3M"/>
    <dbReference type="PDBsum" id="1S3N"/>
    <dbReference type="PDBsum" id="2AHD"/>
    <dbReference type="AlphaFoldDB" id="Q58346"/>
    <dbReference type="SMR" id="Q58346"/>
    <dbReference type="STRING" id="243232.MJ_0936"/>
    <dbReference type="EnsemblBacteria" id="AAB98941">
        <property type="protein sequence ID" value="AAB98941"/>
        <property type="gene ID" value="MJ_0936"/>
    </dbReference>
    <dbReference type="GeneID" id="1451832"/>
    <dbReference type="KEGG" id="mja:MJ_0936"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_4_0_2"/>
    <dbReference type="InParanoid" id="Q58346"/>
    <dbReference type="OMA" id="IHCGDFV"/>
    <dbReference type="OrthoDB" id="78782at2157"/>
    <dbReference type="PhylomeDB" id="Q58346"/>
    <dbReference type="BRENDA" id="3.1.4.1">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58346"/>
    <dbReference type="EvolutionaryTrace" id="Q58346"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155612" description="Phosphodiesterase MJ0936">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
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            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
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        <ligand>
            <name>Ni(2+)</name>
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            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
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        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 4">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="2"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="13"/>
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    </feature>
    <feature type="strand" evidence="6">
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    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="15128743"/>
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    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007744" key="3">
        <source>
            <dbReference type="PDB" id="1S3L"/>
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    </evidence>
    <evidence type="ECO:0007744" key="4">
        <source>
            <dbReference type="PDB" id="1S3M"/>
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    </evidence>
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        <source>
            <dbReference type="PDB" id="1S3N"/>
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    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1S3L"/>
        </source>
    </evidence>
    <sequence length="166" mass="19039" checksum="1F92D7A501EEA315" modified="1996-11-01" version="1">MKIGIMSDTHDHLPNIRKAIEIFNDENVETVIHCGDFVSLFVIKEFENLNANIIATYGNNDGERCKLKEWLKDINEENIIDDFISVEIDDLKFFITHGHHQSVLEMAIKSGLYDVVIYGHTHERVFEEVDDVLVINPGECCGYLTGIPTIGILDTEKKEYREIVLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58365</accession>
    <name>HIS8_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Histidinol-phosphate aminotransferase</fullName>
            <ecNumber>2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisC</name>
        <name type="ordered locus">MJ0955</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2-oxoglutarate + L-histidinol phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64419">
        <property type="entry name" value="C64419"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58365"/>
    <dbReference type="SMR" id="Q58365"/>
    <dbReference type="STRING" id="243232.MJ_0955"/>
    <dbReference type="EnsemblBacteria" id="AAB98960">
        <property type="protein sequence ID" value="AAB98960"/>
        <property type="gene ID" value="MJ_0955"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0955"/>
    <dbReference type="eggNOG" id="arCOG04273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_3_3_2"/>
    <dbReference type="InParanoid" id="Q58365"/>
    <dbReference type="OMA" id="IWLNANE"/>
    <dbReference type="PhylomeDB" id="Q58365"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153496" description="Histidinol-phosphate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="373" mass="42962" checksum="369DB5EB4C671218" modified="1996-11-01" version="1">MGDWMIENKVRDVVKKLKPYVPGKSKEEIARAYGIKPEDIIKLGSNENPWGPSPKIKEKILDEIDKIHQYPEPVNPILMKELSKFLNVDEENIIVGGDGADEIIDTIFRTFVDDGDEVIIPIPTFTQYRVSATIHNAKIKYAKYDKEKDFKLNVESVLNNITDKTKVIFLCTPNNPTGNIIENRDVERVINETDALVVIDHAYIEYAKKEYDWTQKAPEYDNVIVLRTFSKVFGLAGMRVGYGVANKKIIDYMMRVKPIFSLTRLSQVCAITALRDREFFERCVRDGIKSREMLYNGLKKFKDIKVYPSEANYLLVELKTMKAKEFCEELLKRGVIVRDCTSFDGLGDNYVRVSIGTFEEVERFLKILEEIIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58394</accession>
    <name>PFDB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pfdB</name>
        <name type="ordered locus">MJ0987</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64423">
        <property type="entry name" value="C64423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496690.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58394"/>
    <dbReference type="SMR" id="Q58394"/>
    <dbReference type="STRING" id="243232.MJ_0987"/>
    <dbReference type="EnsemblBacteria" id="AAB98989">
        <property type="protein sequence ID" value="AAB98989"/>
        <property type="gene ID" value="MJ_0987"/>
    </dbReference>
    <dbReference type="GeneID" id="1451885"/>
    <dbReference type="KEGG" id="mja:MJ_0987"/>
    <dbReference type="eggNOG" id="arCOG01342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131909_0_1_2"/>
    <dbReference type="InParanoid" id="Q58394"/>
    <dbReference type="OMA" id="PPQVQAM"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000124860" description="Prefoldin subunit beta">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="113" mass="13367" checksum="74982CA40FF4BEFB" modified="2001-01-11" version="2">MELPPQIQAQLMQLQQLQQQLQMILMQKQSVETELKECKKALEELEKSSSDEVYKLVGGLFVKRKKEDVKKELEEKVETLELRVKTLEKQEEKLQSRLKELQEKIQKMIPTAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58428</accession>
    <name>ARCS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Archaeosine synthase</fullName>
            <ecNumber evidence="2">2.6.1.97</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Glutamine:preQ0-tRNA amidinotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">arcS</name>
        <name type="ordered locus">MJ1022</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="J. Biol. Chem." volume="285" first="12706" last="12713">
            <title>Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA.</title>
            <authorList>
                <person name="Phillips G."/>
                <person name="Chikwana V.M."/>
                <person name="Maxwell A."/>
                <person name="El-Yacoubi B."/>
                <person name="Swairjo M.A."/>
                <person name="Iwata-Reuyl D."/>
                <person name="de Crecy-Lagard V."/>
            </authorList>
            <dbReference type="PubMed" id="20129918"/>
            <dbReference type="DOI" id="10.1074/jbc.M110.102236"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Is responsible for the final step in the biosynthesis of archaeosine, a modified nucleoside present in the dihydrouridine loop (D-loop) of archaeal tRNA. Catalyzes the conversion of 7-cyano-7-deazaguanine (preQ0)-modified tRNA to archaeosine-tRNA, transforming a nitrile group to a formamidine group. Can use either glutamine, asparagine or ammonium as amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-cyano-7-carbaguanosine(15) in tRNA + H2O + L-glutamine = archaeosine(15) in tRNA + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:54084"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:14170"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="ChEBI" id="CHEBI:138803"/>
            <dbReference type="EC" id="2.6.1.97"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="2">Optimum temperature is around 40 degrees Celsius. Activity decreases significantly at temperatures above 45 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="5">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeosine synthase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.97" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64427">
        <property type="entry name" value="E64427"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58428"/>
    <dbReference type="SMR" id="Q58428"/>
    <dbReference type="STRING" id="243232.MJ_1022"/>
    <dbReference type="PRIDE" id="Q58428"/>
    <dbReference type="EnsemblBacteria" id="AAB99026">
        <property type="protein sequence ID" value="AAB99026"/>
        <property type="gene ID" value="MJ_1022"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1022"/>
    <dbReference type="eggNOG" id="arCOG00990">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029831_0_0_2"/>
    <dbReference type="InParanoid" id="Q58428"/>
    <dbReference type="OMA" id="YPAAHYD"/>
    <dbReference type="PhylomeDB" id="Q58428"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-16209"/>
    <dbReference type="BRENDA" id="2.6.1.97">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002948">
        <property type="term" value="F:archaeosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002927">
        <property type="term" value="P:archaeosine-tRNA biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="SSF52141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000107146" description="Archaeosine synthase">
        <location>
            <begin position="1"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="495"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="20129918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="20129918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="20129918"/>
        </source>
    </evidence>
    <sequence length="569" mass="65582" checksum="E29EC27908E366ED" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58431</accession>
    <name>CYDE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">L-cysteine desulfidase</fullName>
            <ecNumber evidence="1">4.4.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>L-cysteine desulfhydrase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1025</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochemistry" volume="44" first="1659" last="1670">
            <title>L-cysteine desulfidase: an [4Fe-4S] enzyme isolated from Methanocaldococcus jannaschii that catalyzes the breakdown of L-cysteine into pyruvate, ammonia, and sulfide.</title>
            <authorList>
                <person name="Tchong S.I."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="15683250"/>
            <dbReference type="DOI" id="10.1021/bi0484769"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>COFACTOR</scope>
        <scope>MUTAGENESIS OF CYS-25; HIS-139; CYS-282; CYS-322; ASP-323 AND CYS-329</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cleavage of L-cysteine to form 2-aminoprop-2-enoate and sulfide. The former then spontaneously hydrolyzes to pyruvate and NH(3). May be responsible for the production of sulfide required for the biosynthesis of iron-sulfur centers in this archaea. Is very specific for L-cysteine, with no activity being detected with D-cysteine, L-homocysteine, 3-mercaptopropionate (cysteine without the amino group), cysteamine (cysteine without the carboxylate), or mercaptolactate (the hydroxyl analog of cysteine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-cysteine = H(+) + hydrogen sulfide + NH4(+) + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:24931"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="EC" id="4.4.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">1.78 mM for L-cysteine (at 65 degrees Celsius)</KM>
            <text evidence="1">kcat is 39.6 sec(-1) (at 65 degrees Celsius).</text>
        </kinetics>
        <phDependence>
            <text evidence="1">Optimum pH is 7.6.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the L-cysteine desulfidase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.28" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64427">
        <property type="entry name" value="H64427"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58431"/>
    <dbReference type="STRING" id="243232.MJ_1025"/>
    <dbReference type="EnsemblBacteria" id="AAB99029">
        <property type="protein sequence ID" value="AAB99029"/>
        <property type="gene ID" value="MJ_1025"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1025"/>
    <dbReference type="eggNOG" id="arCOG05065">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051840_0_0_2"/>
    <dbReference type="InParanoid" id="Q58431"/>
    <dbReference type="OMA" id="GAMIPVM"/>
    <dbReference type="PhylomeDB" id="Q58431"/>
    <dbReference type="BRENDA" id="4.4.1.28">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080146">
        <property type="term" value="F:L-cysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019450">
        <property type="term" value="P:L-cysteine catabolic process to pyruvate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005130">
        <property type="entry name" value="Ser_deHydtase-like_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021144">
        <property type="entry name" value="UPF0597"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30501">
        <property type="entry name" value="PTHR30501"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03313">
        <property type="entry name" value="SDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006054">
        <property type="entry name" value="UCP006054"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107148" description="L-cysteine desulfidase">
        <location>
            <begin position="1"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="No activity." evidence="1">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Almost no effect." evidence="1">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Retains 5% of wild-type activity." evidence="1">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No activity; when associated with Ala-329." evidence="1">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Retains 10% of wild-type activity." evidence="1">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Retains 50% of wild-type activity." evidence="1">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No activity; when associated with Ala-322." evidence="1">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="329"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Retains 10% of wild-type activity." evidence="1">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="15683250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="15683250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="15683250"/>
        </source>
    </evidence>
    <sequence length="388" mass="42645" checksum="80CA180E1061315C" modified="1996-11-01" version="1">MVSIMNKNELITEILKNEVVKALGCTEVGLIGYTVAKAKPEDLYSIKEIKLILDKGTFKNAFSVGVPNTNKFGILPAVVGGLLGREENKLEVFKDIKYDEKLEEFIENKLKIEVIDSDVYCKVIIKANKVYEAETKGSHSGKSLSDDLKNAYKSLTLKDFIDYIEDIPEEVIKIIKETIETNKNLSTPEVPEDFISLDLKDEILNHMLKKTVSAVYNRMIGINKPAMAIAGSGNMGLTATLPIIAYDEIKGHDEEKLTKSITLSALTTIYSAYHSSYISAMCGCVNRGGIGAVSGLSYYIFGFDRIEESIKSFTANLPGIVCDGGKIGCALKIASGVFAIYLSLFSKVPYTNGIVGKDFKECIENIGKIGKAMKPVDDEIIEILKNKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58442</accession>
    <name>Y1036_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1036</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1036</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64429">
        <property type="entry name" value="C64429"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58442"/>
    <dbReference type="SMR" id="Q58442"/>
    <dbReference type="STRING" id="243232.MJ_1036"/>
    <dbReference type="EnsemblBacteria" id="AAB99045">
        <property type="protein sequence ID" value="AAB99045"/>
        <property type="gene ID" value="MJ_1036"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1036"/>
    <dbReference type="eggNOG" id="arCOG00576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1187785_0_0_2"/>
    <dbReference type="InParanoid" id="Q58442"/>
    <dbReference type="OMA" id="IHTFIDG"/>
    <dbReference type="PhylomeDB" id="Q58442"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107151" description="Uncharacterized protein MJ1036">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="233" mass="26171" checksum="D2D82ACF6200DB58" modified="1996-11-01" version="1">MSNMVEVPIFIAILSFIVMCIGELLAYYSVSLKYKYEFEAISFGFIFGVATLILIPKSYSNMFVLYVILGMITVYLIEKYLAYCPLSKKYCVECDNLEENRIKFIYPISFFIHTFIDGLIIAVSYISEIGLPLYLAILMHKLPAGFVLISPLKGVYKNPLYPGVFVSFGTVLGTIVGLVTLKDVSTKILLAFSGGVFLGAFLMLAPHIYEHKEEKTFLYILLGYILVGIIALH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q58449</accession>
    <name>Y1049_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1049</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1049</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64430">
        <property type="entry name" value="H64430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870562.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58449"/>
    <dbReference type="EnsemblBacteria" id="AAB99059">
        <property type="protein sequence ID" value="AAB99059"/>
        <property type="gene ID" value="MJ_1049"/>
    </dbReference>
    <dbReference type="GeneID" id="1451946"/>
    <dbReference type="KEGG" id="mja:MJ_1049"/>
    <dbReference type="eggNOG" id="arCOG05066">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736435_0_0_2"/>
    <dbReference type="OMA" id="PKLYVVR"/>
    <dbReference type="OrthoDB" id="84856at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107152" description="Uncharacterized protein MJ1049">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <sequence length="161" mass="18880" checksum="DFD39C9FA1514D22" modified="1996-11-01" version="1">MVIKKIIKKIRGNKDLPKPIEVPDEEYIVIGEEKPAYILEEESETESEVLGLEHKEKEEVKEVEEVVRVEKILPKLYVVRIKHPLDFENIKDKIPEYDVVIVNFEEVPFESILKELNEFRDYMSILNFKLGFVAENVLLAYRDDVILDKYVSNITDDAENV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="143" xmlns="http://uniprot.org/uniprot">
    <accession>Q58484</accession>
    <name>AROE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="1">SDH</shortName>
            <ecNumber evidence="1">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroE</name>
        <name type="ordered locus">MJ1084</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Structure" volume="11" first="1005" last="1013">
            <title>Crystal structure of shikimate 5-dehydrogenase (SDH) bound to NADP: insights into function and evolution.</title>
            <authorList>
                <person name="Padyana A.K."/>
                <person name="Burley S.K."/>
            </authorList>
            <dbReference type="PubMed" id="12906831"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(03)00159-X"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.35 ANGSTROMS) IN COMPLEX WITH NADP</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64435">
        <property type="entry name" value="C64435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870596.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1NVT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.35 A"/>
        <property type="chains" value="A/B=1-282"/>
    </dbReference>
    <dbReference type="PDBsum" id="1NVT"/>
    <dbReference type="AlphaFoldDB" id="Q58484"/>
    <dbReference type="SMR" id="Q58484"/>
    <dbReference type="STRING" id="243232.MJ_1084"/>
    <dbReference type="EnsemblBacteria" id="AAB99086">
        <property type="protein sequence ID" value="AAB99086"/>
        <property type="gene ID" value="MJ_1084"/>
    </dbReference>
    <dbReference type="GeneID" id="1451980"/>
    <dbReference type="KEGG" id="mja:MJ_1084"/>
    <dbReference type="eggNOG" id="arCOG01033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044063_4_1_2"/>
    <dbReference type="InParanoid" id="Q58484"/>
    <dbReference type="OMA" id="FGNPIKH"/>
    <dbReference type="OrthoDB" id="98644at2157"/>
    <dbReference type="PhylomeDB" id="Q58484"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-18802"/>
    <dbReference type="BRENDA" id="1.1.1.25">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58484"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminiacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="PTHR21089"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="SSF53223"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136060" description="Shikimate dehydrogenase (NADP(+))">
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    <sequence length="282" mass="30877" checksum="E7398E5D642F7BD1" modified="1996-11-01" version="1">MINAKTKVIGLIGHPVEHSFSPIMHNAAFKDKGLNYVYVAFDVLPENLKYVIDGAKALGIVGFNVTIPHKIEIMKYLDEIDKDAQLIGAVNTIKIEDGKAIGYNTDGIGARMALEEEIGRVKDKNIVIYGAGGAARAVAFELAKDNNIIIANRTVEKAEALAKEIAEKLNKKFGEEVKFSGLDVDLDGVDIIINATPIGMYPNIDVEPIVKAEKLREDMVVMDLIYNPLETVLLKEAKKVNAKTINGLGMLIYQGAVAFKIWTGVEPNIEVMKNAIIDKITK</sequence>
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<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58493</accession>
    <name>NIFB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FeMo cofactor biosynthesis protein NifB</fullName>
            <ecNumber>4.-.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Nitrogenase cofactor maturase NifB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Radical SAM assemblase NifB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nifB</name>
        <name type="ordered locus">MJ1093</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co or M-cluster) found in the dinitrogenase enzyme of the nitrogenase complex in nitrogen-fixing microorganisms. NifB catalyzes the crucial step of radical SAM-dependent carbide insertion that occurs concomitant with the insertion of a 9th sulfur and the rearrangement/coupling of two [4Fe-4S] clusters into a [8Fe-9S-C] cluster, the precursor to the M-cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 3 [4Fe-4S] clusters per monomer. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine. The two others probably act as substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the radical SAM superfamily. NifB family.</text>
    </comment>
    <dbReference type="EC" id="4.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D64436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870605.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58493"/>
    <dbReference type="SMR" id="Q58493"/>
    <dbReference type="STRING" id="243232.MJ_1093"/>
    <dbReference type="EnsemblBacteria" id="AAB99096">
        <property type="protein sequence ID" value="AAB99096"/>
        <property type="gene ID" value="MJ_1093"/>
    </dbReference>
    <dbReference type="GeneID" id="1451989"/>
    <dbReference type="KEGG" id="mja:MJ_1093"/>
    <dbReference type="eggNOG" id="arCOG00956">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027639_1_0_2"/>
    <dbReference type="InParanoid" id="Q58493"/>
    <dbReference type="OMA" id="PCYNEKL"/>
    <dbReference type="OrthoDB" id="46264at2157"/>
    <dbReference type="PhylomeDB" id="Q58493"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
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    <dbReference type="InterPro" id="IPR005980">
        <property type="entry name" value="Nase_CF_NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="PTHR43787:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0535">Nitrogen fixation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000153050" description="FeMo cofactor biosynthesis protein NifB">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="24"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
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        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
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        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
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        <location>
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            <name>S-adenosyl-L-methionine</name>
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        </ligand>
    </feature>
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            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="196"/>
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        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
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            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
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        <location>
            <position position="265"/>
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            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="D5VRM1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P69848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="300" mass="34568" checksum="5EEA1B14CA4CF1E0" modified="1996-11-01" version="1">MDKNKMSKFAHITKVHPCFNEKIHDKVGRVHLPVAPRCNIACKFCRRSLGKEACEHRPGVALSVLKPEDVESYLNKVLKEIPNIKVVGIAGPGDSLFNKETFETLKIIDEKFPNLIKCLSTNGLLLNKYYKKLADLNVKTVTVTVNAIDPEILKEIVEWVYYDKKVHYGIEGAKILIENQIDGIKKAYDEDLIIKINTVLIPEINMNHVVDIAKELKDFVYIQNIIPLIPLYKMSHLRPPTCEELKKVREECEKYIPQFRACGQCRADAVGLIKERKILEEFFKEKNKKLNVFELKHFSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58498</accession>
    <name>Y1098_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1098</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1098</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64437">
        <property type="entry name" value="A64437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870610.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58498"/>
    <dbReference type="SMR" id="Q58498"/>
    <dbReference type="STRING" id="243232.MJ_1098"/>
    <dbReference type="EnsemblBacteria" id="AAB99101">
        <property type="protein sequence ID" value="AAB99101"/>
        <property type="gene ID" value="MJ_1098"/>
    </dbReference>
    <dbReference type="GeneID" id="1451995"/>
    <dbReference type="KEGG" id="mja:MJ_1098"/>
    <dbReference type="HOGENOM" id="CLU_1092434_0_0_2"/>
    <dbReference type="InParanoid" id="Q58498"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03161">
        <property type="entry name" value="LAGLIDADG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107167" description="Uncharacterized protein MJ1098">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <sequence length="254" mass="30440" checksum="E98B050B1340BF34" modified="1996-11-01" version="1">MYEKYIVEGLSDREIAYLIGCGKATVVRARQKHGIYREDVKMCDDYTLDNISEDLRTFIDGLLLGDACITEKGNLLITQNKRYDWLEYVKHRFQQFGLNVYFHCYKYKRRTSEVIADLYVLSTSRYELFRQLRERWYPDGIKRIPNDLVINDEGLAQWYLGDGSLTKQKNGYKLELSTHGFTLDENKFLQQKLKLLYGFDFRISKKHQYRYLRLFKSKQVHAFCSIVEPFIPPSYRNKVRCLHDYQWLKSWDVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58537</accession>
    <name>Y1137_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1137</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1137</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64441">
        <property type="entry name" value="H64441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870648.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58537"/>
    <dbReference type="STRING" id="243232.MJ_1137"/>
    <dbReference type="EnsemblBacteria" id="AAB99139">
        <property type="protein sequence ID" value="AAB99139"/>
        <property type="gene ID" value="MJ_1137"/>
    </dbReference>
    <dbReference type="GeneID" id="1452033"/>
    <dbReference type="KEGG" id="mja:MJ_1137"/>
    <dbReference type="eggNOG" id="arCOG01880">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031477_4_2_2"/>
    <dbReference type="InParanoid" id="Q58537"/>
    <dbReference type="OMA" id="CKREKED"/>
    <dbReference type="OrthoDB" id="91246at2157"/>
    <dbReference type="PhylomeDB" id="Q58537"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037997">
        <property type="entry name" value="Dgk1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31303">
        <property type="entry name" value="PTHR31303"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107184" description="Uncharacterized protein MJ1137">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="191" mass="21688" checksum="EB041414C9E41310" modified="1996-11-01" version="1">MREIYRQTIHLVFGVLIAFSVLIFKKQLIIPLIVSIVIGICLYFLCKRYYIPIVSDLLNLCKREKEDGKGAIYFAIGMLISLILIDDIKAVFFGILVFAVGDSLATIIGIRGKLKIKYFGKTVEGFLAFFISASLILYPFYGTYGIFVALISAFIEFVSKKIRIDDNLYLPFIVAFIINHQINICSLMNFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58539</accession>
    <name>RNP3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 3</fullName>
            <shortName evidence="1">RNase P component 3</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp3</name>
        <name type="ordered locus">MJ1139</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Nucleic Acids Res." volume="36" first="4172" last="4180">
            <title>Studies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysis.</title>
            <authorList>
                <person name="Pulukkunat D.K."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="18558617"/>
            <dbReference type="DOI" id="10.1093/nar/gkn360"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP2</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="411" first="368" last="383">
            <title>Cooperative RNP assembly: complementary rescue of structural defects by protein and RNA subunits of archaeal RNase P.</title>
            <authorList>
                <person name="Chen W.Y."/>
                <person name="Xu Y."/>
                <person name="Cho I.M."/>
                <person name="Oruganti S.V."/>
                <person name="Foster M.P."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="21683084"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2011.05.012"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP2</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2012" name="Nucleic Acids Res." volume="40" first="4666" last="4680">
            <title>Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors.</title>
            <authorList>
                <person name="Chen W.Y."/>
                <person name="Singh D."/>
                <person name="Lai L.B."/>
                <person name="Stiffler M.A."/>
                <person name="Lai H.D."/>
                <person name="Foster M.P."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="22298511"/>
            <dbReference type="DOI" id="10.1093/nar/gks013"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP2</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 4">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3 4">Consists of a catalytic RNA component and at least 4-5 protein subunits. Forms a subcomplex with Rnp2 which stimulates the catalytic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64442">
        <property type="entry name" value="B64442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870650.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6K0A">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.60 A"/>
        <property type="chains" value="C/D=1-232"/>
    </dbReference>
    <dbReference type="PDB" id="6K0B">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.30 A"/>
        <property type="chains" value="C/D=1-232"/>
    </dbReference>
    <dbReference type="PDBsum" id="6K0A"/>
    <dbReference type="PDBsum" id="6K0B"/>
    <dbReference type="AlphaFoldDB" id="Q58539"/>
    <dbReference type="SMR" id="Q58539"/>
    <dbReference type="STRING" id="243232.MJ_1139"/>
    <dbReference type="PRIDE" id="Q58539"/>
    <dbReference type="EnsemblBacteria" id="AAB99146">
        <property type="protein sequence ID" value="AAB99146"/>
        <property type="gene ID" value="MJ_1139"/>
    </dbReference>
    <dbReference type="GeneID" id="1452035"/>
    <dbReference type="KEGG" id="mja:MJ_1139"/>
    <dbReference type="eggNOG" id="arCOG00307">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074509_0_0_2"/>
    <dbReference type="InParanoid" id="Q58539"/>
    <dbReference type="OMA" id="INRAACE"/>
    <dbReference type="OrthoDB" id="66325at2157"/>
    <dbReference type="PhylomeDB" id="Q58539"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00756">
        <property type="entry name" value="RNase_P_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023539">
        <property type="entry name" value="RNase_P_comp-3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000140039" description="Ribonuclease P protein component 3">
        <location>
            <begin position="1"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18558617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="21683084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22298511"/>
        </source>
    </evidence>
    <sequence length="232" mass="27346" checksum="5490A2447A7E897B" modified="1996-11-01" version="1">MRIDINRIEKEEDIKLLKELKWNGFVFYQYDDEFSKDRYEEVKAIAESYKLKVYSGVKIKTESSKQLRDKVKKFRNKCHIILIEGGVLKINRAAVELHDVDILSTPELGRKDSGIDHVLARLASNHRVAIELNFKTLLNKDGYERARTLLFFRNNLKLAKKFDVPVVISTDAENKYQIKNPYDLRAFLNTLVEPLYAKKIMETAYKICDFRDYLMRDNVVRYGVEIIKEEKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58545</accession>
    <name>Y1145_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1145</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1145</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64442">
        <property type="entry name" value="H64442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870656.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58545"/>
    <dbReference type="SMR" id="Q58545"/>
    <dbReference type="STRING" id="243232.MJ_1145"/>
    <dbReference type="EnsemblBacteria" id="AAB99155">
        <property type="protein sequence ID" value="AAB99155"/>
        <property type="gene ID" value="MJ_1145"/>
    </dbReference>
    <dbReference type="GeneID" id="1452041"/>
    <dbReference type="KEGG" id="mja:MJ_1145"/>
    <dbReference type="eggNOG" id="arCOG05069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194236_0_0_2"/>
    <dbReference type="OMA" id="YWACQAY"/>
    <dbReference type="OrthoDB" id="124040at2157"/>
    <dbReference type="PhylomeDB" id="Q58545"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014009" description="Uncharacterized protein MJ1145">
        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="65" mass="7693" checksum="1DD7EF92E847F51F" modified="1996-11-01" version="1" precursor="true">MDDIDRKAISLLMDATLMSEDEIERTLKILRNMARIKKRKEKNLKSIRDVLDYWACQAYKSSMKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58551</accession>
    <name>Y1151_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1151</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1151</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64443">
        <property type="entry name" value="F64443"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58551"/>
    <dbReference type="SMR" id="Q58551"/>
    <dbReference type="STRING" id="243232.MJ_1151"/>
    <dbReference type="PRIDE" id="Q58551"/>
    <dbReference type="EnsemblBacteria" id="AAB99157">
        <property type="protein sequence ID" value="AAB99157"/>
        <property type="gene ID" value="MJ_1151"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1151"/>
    <dbReference type="eggNOG" id="arCOG10934">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2127834_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107189" description="Uncharacterized protein MJ1151">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <sequence length="113" mass="14013" checksum="EDC0A02B7BA09293" modified="1996-11-01" version="1">MIIMKPRNKRNKKFKKPEWLIEVEDKIEKMADEEFDKLFEEIYEKLNRGGLREVLNVHKTDKSKYKKYKKPKWLIEAEKELEKAIETGEYFKWLKEQGEKYRKQKKEIKKNNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58574</accession>
    <name>PYRG_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CTP synthase</fullName>
            <ecNumber evidence="1">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="1">CTP synthetase</shortName>
            <shortName evidence="1">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrG</name>
        <name type="ordered locus">MJ1174</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + UTP = ADP + CTP + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + NH4(+) + UTP = ADP + CTP + 2 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTP synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64446">
        <property type="entry name" value="E64446"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58574"/>
    <dbReference type="SMR" id="Q58574"/>
    <dbReference type="STRING" id="243232.MJ_1174"/>
    <dbReference type="EnsemblBacteria" id="AAB99177">
        <property type="protein sequence ID" value="AAB99177"/>
        <property type="gene ID" value="MJ_1174"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1174"/>
    <dbReference type="eggNOG" id="arCOG00063">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011675_5_0_2"/>
    <dbReference type="InParanoid" id="Q58574"/>
    <dbReference type="OMA" id="EFNNAYR"/>
    <dbReference type="PhylomeDB" id="Q58574"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03113">
        <property type="entry name" value="CTPS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01746">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01227">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004468">
        <property type="entry name" value="CTP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017456">
        <property type="entry name" value="CTP_synthase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033828">
        <property type="entry name" value="GATase1_CTP_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11550">
        <property type="entry name" value="PTHR11550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06418">
        <property type="entry name" value="CTP_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00337">
        <property type="entry name" value="PyrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138260" description="CTP synthase">
        <location>
            <begin position="1"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="292"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Amidoligase domain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for glutamine hydrolysis" evidence="1">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="513"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="515"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="188"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="188"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <note>allosteric inhibitor</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>UTP</name>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="240"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="360"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="388"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01227"/>
        </source>
    </evidence>
    <sequence length="540" mass="61023" checksum="6DD879FC31DA8A01" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58655</accession>
    <name>HJA3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable archaeal histone 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1258</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or heterodimer with another histone. Dimers then assemble into higher oligomers, with the DNA wrapped around the protein core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64457">
        <property type="entry name" value="A64457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870771.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58655"/>
    <dbReference type="SMR" id="Q58655"/>
    <dbReference type="STRING" id="243232.MJ_1258"/>
    <dbReference type="EnsemblBacteria" id="AAB99261">
        <property type="protein sequence ID" value="AAB99261"/>
        <property type="gene ID" value="MJ_1258"/>
    </dbReference>
    <dbReference type="GeneID" id="1452156"/>
    <dbReference type="KEGG" id="mja:MJ_1258"/>
    <dbReference type="eggNOG" id="arCOG02144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="InParanoid" id="Q58655"/>
    <dbReference type="OMA" id="ELPVAPC"/>
    <dbReference type="PhylomeDB" id="Q58655"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154989" description="Probable archaeal histone 3">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P19267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="67" mass="7245" checksum="802E4C34E239DAB7" modified="1997-11-01" version="1">MAELPVAPCVRILKKAGAQRVSEAAGKYFAEALEEIALEIARKSVDLAKHAKRKTVKVEDVKAALRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58678</accession>
    <name>Y1282_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0252 protein MJ1282</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1282</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0252 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64460">
        <property type="entry name" value="A64460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870795.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58678"/>
    <dbReference type="SMR" id="Q58678"/>
    <dbReference type="STRING" id="243232.MJ_1282"/>
    <dbReference type="DNASU" id="1452180"/>
    <dbReference type="EnsemblBacteria" id="AAB99291">
        <property type="protein sequence ID" value="AAB99291"/>
        <property type="gene ID" value="MJ_1282"/>
    </dbReference>
    <dbReference type="GeneID" id="1452180"/>
    <dbReference type="KEGG" id="mja:MJ_1282"/>
    <dbReference type="eggNOG" id="arCOG02164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_786686_0_0_2"/>
    <dbReference type="OMA" id="IENIAKW"/>
    <dbReference type="OrthoDB" id="4820at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007562">
        <property type="entry name" value="Transglutaminase-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04473">
        <property type="entry name" value="DUF553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159555" description="UPF0252 protein MJ1282">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="352" mass="40310" checksum="41FF73286AB1FFA8" modified="1996-11-01" version="1">MRKIILIFFIFLILQNIYAYEKIYDVNHVCYYTLTSVELQKFVKTAEIITPLNADNKTTITKDTILVGNPEENPLTKKYIGFFKIKINKTFPGKNKGIIEKQIINGHTVILLAGSDIQGTYASIITFANLNDIPENPIICETSNKVNIYSTSLNSEYFRDFIEKNILTPKEIEKVKSLSYKLKGKDKKATIENIAKWVANNIKYDYDKCKKIESGKFSWDEYNTPSETVKTKKGVCLDYATLTSALLLNDNIIPYMLDVALYNTSSLKISSYHASVAVKIDNTYFVIDQQPYLIPINEYTAQTFEDNLRIASIVMFRVVKERDGIKLIKEKEVPGIAIYGDLINLLEMRFNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58759</accession>
    <name>TRUD2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase D 2</fullName>
            <ecNumber evidence="1">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase D 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase D 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truD2</name>
        <name type="ordered locus">MJ1364</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99372" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99372.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64470">
        <property type="entry name" value="C64470"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58759"/>
    <dbReference type="SMR" id="Q58759"/>
    <dbReference type="STRING" id="243232.MJ_1364"/>
    <dbReference type="EnsemblBacteria" id="AAB99372">
        <property type="protein sequence ID" value="AAB99372"/>
        <property type="gene ID" value="MJ_1364"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1364"/>
    <dbReference type="eggNOG" id="arCOG04252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="InParanoid" id="Q58759"/>
    <dbReference type="OMA" id="IGYCGLK"/>
    <dbReference type="PhylomeDB" id="Q58759"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="PTHR13326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000152541" description="Probable tRNA pseudouridine synthase D 2">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="TRUD" evidence="1">
        <location>
            <begin position="160"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="422" mass="50180" checksum="BDADF2BBA901F6E4" modified="2003-09-26" version="2">MKNISTKESFFKFKFNNRSLYCWGAFMKLRMKPEDFIVEEIIDFNKIAGDRCYLYKLTKRNIESLKAFSYIAKKFKIPLKDIGYCGLKDRHALTTQYISIPKKYGKLSLDEPNLKLELIGESKFLLLGDLEGNRFTITVRGLKKEDIPKIKENLKYLEFGAPNYFDSQRFGSVFDKKFIAKEVIKGNYEEAVKILLTKYKKSEKKLIKDLKRFIDKNWGDWDKIWEYIKENNIKSRLYVNMVKELKKSNDYKKALSYVDDRLKKIFVAAYQSYLWNECVKELLRKYVPEEDRVYYEYECGTLMFYKKMDEEVFNILKDKKFPTIAPDIEYSGEEKEIIEEILKREGLTMEELNNIGELGKFIYSERKILSIPKNLKIGEFEEDELNKGKYKITLSYELEKGSYATIIIKRAFLGVKTKKRKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58772</accession>
    <name>SYE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="1">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gltX</name>
        <name type="ordered locus">MJ1377</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64471">
        <property type="entry name" value="H64471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870894.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58772"/>
    <dbReference type="SMR" id="Q58772"/>
    <dbReference type="STRING" id="243232.MJ_1377"/>
    <dbReference type="EnsemblBacteria" id="AAB99384">
        <property type="protein sequence ID" value="AAB99384"/>
        <property type="gene ID" value="MJ_1377"/>
    </dbReference>
    <dbReference type="GeneID" id="1452280"/>
    <dbReference type="KEGG" id="mja:MJ_1377"/>
    <dbReference type="eggNOG" id="arCOG04302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_1_3_2"/>
    <dbReference type="InParanoid" id="Q58772"/>
    <dbReference type="OMA" id="MRFAPNP"/>
    <dbReference type="OrthoDB" id="8922at2157"/>
    <dbReference type="PhylomeDB" id="Q58772"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_L25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_L25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="SSF50715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119717" description="Glutamate--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <sequence length="553" mass="64577" checksum="B19D4177990454A0" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-04" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58782</accession>
    <name>Y1387_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative nickel insertion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1387</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LarC family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64473">
        <property type="entry name" value="B64473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870904.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58782"/>
    <dbReference type="SMR" id="Q58782"/>
    <dbReference type="STRING" id="243232.MJ_1387"/>
    <dbReference type="EnsemblBacteria" id="AAB99396">
        <property type="protein sequence ID" value="AAB99396"/>
        <property type="gene ID" value="MJ_1387"/>
    </dbReference>
    <dbReference type="GeneID" id="1452290"/>
    <dbReference type="KEGG" id="mja:MJ_1387"/>
    <dbReference type="eggNOG" id="arCOG02701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028523_2_1_2"/>
    <dbReference type="InParanoid" id="Q58782"/>
    <dbReference type="OMA" id="EILCKHE"/>
    <dbReference type="OrthoDB" id="69338at2157"/>
    <dbReference type="PhylomeDB" id="Q58782"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01074">
        <property type="entry name" value="LarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002822">
        <property type="entry name" value="Ni_insertion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566">
        <property type="entry name" value="PTHR36566"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01969">
        <property type="entry name" value="DUF111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00299">
        <property type="entry name" value="TIGR00299"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146860" description="Putative nickel insertion protein">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01074"/>
        </source>
    </evidence>
    <sequence length="379" mass="42868" checksum="9BDB73BE619B4EFD" modified="1996-11-01" version="1">MFLLDPFSGISGDMFLSAMIDFVDKEDFINTIKKVIDVEIEIKKVKKCHILANKVNIIPKCINCNANTYKDIKNVIKSSDIQEDIKITALEILKILAEAESKVHNVDVENVHFHEVGNYDTIADIVGAAYIINKLNLKNNCLYKPINVGNGFVRTEHGLLPVPAPATAEILKGLKIFFSDINEELTTPTGSAIIKYINPKLAKGAFIIKEVSYGAGDKDLNLLNALRVFRVEDIKREDIVLLETNVDDISAEILGYLYEVLDGKVRDLHFIPTYMKKNRPAYTIRAIVDRDIAEEVAKIIMRETGSLGVRIFDIERITADREFKTIKLFDESVRLKVGRVNDEIISQKPEFEDLKNIAKKYGIPLKDLYKLINISQIKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q58787</accession>
    <name>CIMA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>(R)-citramalate synthase CimA</fullName>
            <ecNumber>2.3.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cimA</name>
        <name type="ordered locus">MJ1392</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Bacteriol." volume="181" first="331" last="333">
            <title>(R)-citramalate synthase in methanogenic archaea.</title>
            <authorList>
                <person name="Howell D.M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="9864346"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-10</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>acetyl-CoA + H2O + pyruvate = (3R)-citramalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19045"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.182"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.182"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64473">
        <property type="entry name" value="G64473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870909.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58787"/>
    <dbReference type="SMR" id="Q58787"/>
    <dbReference type="STRING" id="243232.MJ_1392"/>
    <dbReference type="EnsemblBacteria" id="AAB99402">
        <property type="protein sequence ID" value="AAB99402"/>
        <property type="gene ID" value="MJ_1392"/>
    </dbReference>
    <dbReference type="GeneID" id="1452295"/>
    <dbReference type="KEGG" id="mja:MJ_1392"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="InParanoid" id="Q58787"/>
    <dbReference type="OMA" id="SNMFAHE"/>
    <dbReference type="OrthoDB" id="10632at2157"/>
    <dbReference type="PhylomeDB" id="Q58787"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-11899"/>
    <dbReference type="BRENDA" id="2.3.1.182">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01028">
        <property type="entry name" value="CimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024890">
        <property type="entry name" value="Citramalate_synthase_CimA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000140450" description="(R)-citramalate synthase CimA">
        <location>
            <begin position="1"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AA sequence." evidence="3" ref="2">
        <original>M</original>
        <variation>L</variation>
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="9864346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="491" mass="53493" checksum="9D4FA0EA6F8F3BE5" modified="1997-11-01" version="1">MMVRIFDTTLRDGEQTPGVSLTPNDKLEIAKKLDELGVDVIEAGSAITSKGEREGIKLITKEGLNAEICSFVRALPVDIDAALECDVDSVHLVVPTSPIHMKYKLRKTEDEVLETALKAVEYAKEHGLIVELSAEDATRSDVNFLIKLFNEGEKVGADRVCVCDTVGVLTPQKSQELFKKITENVNLPVSVHCHNDFGMATANTCSAVLGGAVQCHVTVNGIGERAGNASLEEVVAALKILYGYDTKIKMEKLYEVSRIVSRLMKLPVPPNKAIVGDNAFAHEAGIHVDGLIKNTETYEPIKPEMVGNRRRIILGKHSGRKALKYKLDLMGINVSDEQLNKIYERVKEFGDLGKYISDADLLAIVREVTGKLVEEKIKLDELTVVSGNKITPIASVKLHYKGEDITLIETAYGVGPVDAAINAVRKAISGVADIKLVEYRVEAIGGGTDALIEVVVKLRKGTEIVEVRKSDADIIRASVDAVMEGINMLLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58800</accession>
    <name>Y1405_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Acylphosphatase-like protein MJ1405</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1405</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64475">
        <property type="entry name" value="D64475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58800"/>
    <dbReference type="SMR" id="Q58800"/>
    <dbReference type="STRING" id="243232.MJ_1405"/>
    <dbReference type="EnsemblBacteria" id="AAB99422">
        <property type="protein sequence ID" value="AAB99422"/>
        <property type="gene ID" value="MJ_1405"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1405"/>
    <dbReference type="eggNOG" id="arCOG01674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2662348_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58800"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="PTHR47268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158565" description="Acylphosphatase-like protein MJ1405">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <sequence length="75" mass="8950" checksum="10A601F869514B90" modified="1996-11-01" version="1">MVKFMPTTYEIIIYGRIQHVGFRERIENLGHALGIDGIVYNYEDGTVRILANFPSERKKKLFKDKTNFWRVRKCK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58803</accession>
    <name>Y1408_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1408</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1408</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870925.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58803"/>
    <dbReference type="SMR" id="Q58803"/>
    <dbReference type="STRING" id="243232.MJ_1408"/>
    <dbReference type="EnsemblBacteria" id="AAB99416">
        <property type="protein sequence ID" value="AAB99416"/>
        <property type="gene ID" value="MJ_1408"/>
    </dbReference>
    <dbReference type="GeneID" id="1452311"/>
    <dbReference type="KEGG" id="mja:MJ_1408"/>
    <dbReference type="eggNOG" id="arCOG00352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011784_0_0_2"/>
    <dbReference type="InParanoid" id="Q58803"/>
    <dbReference type="OMA" id="DPSEYCG"/>
    <dbReference type="OrthoDB" id="66209at2157"/>
    <dbReference type="PhylomeDB" id="Q58803"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000195036" description="Uncharacterized protein MJ1408">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="1">
        <location>
            <begin position="171"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="177"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="219"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="286"/>
            <end position="289"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01047"/>
        </source>
    </evidence>
    <sequence length="350" mass="40006" checksum="C6B02B7B6965FE99" modified="1998-06-01" version="2">MSREANPFKKMPTILMPDELMAKALRRGEKVANEMRQKELPWLLKARFVEEHKVRTIASVVADNLQKVIDKTPPVRKLPKFYQEMVEVLVGIDDFKKSMGAFKWASELVRKLGNEYARKIRKARTPQQAGKLRKEFVGRVKSILEQIHPEMAFVAVAREKLKDLPTFKDLPTVVIAGYPNVGKSTLLKKLTGADVEINSYPFTTKGINVGYIGEIQMVDTPGLLDRPLYERNDIELQAILALNYLANLILFIIDASEFCGYTIEEQINLLKEIKDLFKAPIVVAINKIDLVDEERVKEIEEKLKEVGIEEILKISADKDIGLDELKERLKKIAIKEFLKDKDAENKELEC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58855</accession>
    <name>Y1460_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1460</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1460</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the M.jannaschii MJ0150/MJ0739/MJ0745/MJ1460/MJ1642 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64482">
        <property type="entry name" value="C64482"/>
    </dbReference>
    <dbReference type="PDB" id="2OSD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-162"/>
    </dbReference>
    <dbReference type="PDB" id="2OSO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-162"/>
    </dbReference>
    <dbReference type="PDBsum" id="2OSD"/>
    <dbReference type="PDBsum" id="2OSO"/>
    <dbReference type="AlphaFoldDB" id="Q58855"/>
    <dbReference type="SMR" id="Q58855"/>
    <dbReference type="DNASU" id="1452364"/>
    <dbReference type="EnsemblBacteria" id="AAB99471">
        <property type="protein sequence ID" value="AAB99471"/>
        <property type="gene ID" value="MJ_1460"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1460"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128702_1_0_2"/>
    <dbReference type="InParanoid" id="Q58855"/>
    <dbReference type="OMA" id="KIFKLMN"/>
    <dbReference type="PhylomeDB" id="Q58855"/>
    <dbReference type="EvolutionaryTrace" id="Q58855"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107349" description="Uncharacterized protein MJ1460">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="8"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="18"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="82"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="87"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="97"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="133"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2OSO"/>
        </source>
    </evidence>
    <sequence length="162" mass="18783" checksum="1A50EDE6483E424D" modified="1996-11-01" version="1">MAFMEKIFPDILEAIRNEEIIKESKKIPMPYFGLFALVIFDKVKELGSETSLYEIGEEFGKMLSPKNIEELKKIFKLMNFGDLEIDENKILLKNPPYKIKLSNPPYQWVSKEEPIHDFIAGILAGCLEEIFKKKFVVNEVECVSQGKDKCVFEVKEVDELNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58873</accession>
    <name>TTUA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-5-methyluridine(54) 2-sulfurtransferase</fullName>
            <ecNumber evidence="1">2.8.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA thiouridine synthetase TtuA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1478</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the ATP-dependent 2-thiolation of 5-methyluridine residue at position 54 in the T loop of tRNAs, leading to 5-methyl-2-thiouridine (m(5)s(2)U or s(2)T) (By similarity). This modification allows thermal stabilization of tRNAs in thermophilic microorganisms, and is required for cell growth at high temperatures.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyluridine(54) in tRNA + ATP + hydrogen sulfide = 5-methyl-2-thiouridine(54) in tRNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:55188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10167"/>
            <dbReference type="Rhea" id="RHEA-COMP:13344"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:74447"/>
            <dbReference type="ChEBI" id="CHEBI:136799"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues, the fourth Fe with a free coordination site may bind a small ligand, such as exogenous sulfide, thus acting as a sulfur carrier.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">In TtuA from T.thermophilus, the sulfur inserted into the nucleoside comes from the C-terminal thiocarboxylate of TtuB, but there is no TtuB ortholog in M.jannaschii.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The thiolation reaction likely consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by the hydrosulfide attached to the Fe-S cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TtcA family. TtuA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64484">
        <property type="entry name" value="E64484"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58873"/>
    <dbReference type="SMR" id="Q58873"/>
    <dbReference type="STRING" id="243232.MJ_1478"/>
    <dbReference type="EnsemblBacteria" id="AAB99482">
        <property type="protein sequence ID" value="AAB99482"/>
        <property type="gene ID" value="MJ_1478"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1478"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_1_2"/>
    <dbReference type="InParanoid" id="Q58873"/>
    <dbReference type="OMA" id="CLHINLG"/>
    <dbReference type="PhylomeDB" id="Q58873"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00269">
        <property type="entry name" value="TIGR00269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000219892" description="tRNA-5-methyluridine(54) 2-sulfurtransferase">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O58038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q72LF3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="303" mass="35543" checksum="D62272DDA060A40D" modified="1996-11-01" version="1">MVKTMKCKFCDKKSYIKLKSPKMYLCKEHFVEYFENKVKKSIDKYKMLSKDEKILVAVSGGKDGHAAAWVLKKLGYNIELFHINLGIEGFSEESLKAVKELAEKLEVPLHVVNLKDITGKTMEDIRGKKCSICGTTKRYLMNKFGYENGFDVIVTGHNLDDEVSFILNNLFNWNIRYLAKHEPVLPAHDKFLKKVKIFFEIEEELILKYAEAEEIPYTTVECKYAERAITLKHRAYLNELEKERPGIKYQFLSGYMKNRHLFKVEEEDFQFRECEVCGMTSAGKICSFCRVWKLYKKKKENRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58890</accession>
    <name>Y1495_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1495</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1495</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0137.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64486">
        <property type="entry name" value="F64486"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58890"/>
    <dbReference type="SMR" id="Q58890"/>
    <dbReference type="STRING" id="243232.MJ_1495"/>
    <dbReference type="EnsemblBacteria" id="AAB99506">
        <property type="protein sequence ID" value="AAB99506"/>
        <property type="gene ID" value="MJ_1495"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1495"/>
    <dbReference type="eggNOG" id="arCOG01911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067083_0_0_2"/>
    <dbReference type="InParanoid" id="Q58890"/>
    <dbReference type="OMA" id="AVMGPVD"/>
    <dbReference type="PhylomeDB" id="Q58890"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_sah"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107378" description="Uncharacterized protein MJ1495">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="292" mass="33509" checksum="B8A34C29B3A51246" modified="1997-11-01" version="1">MILIIYLTKIMGDEMTSMDMFFFLFIFLLFIYPEMMMRYRIMKRLRCIREIERQRGTRVIAMIHRQEALTFLGIPIYKFITIEDSEEILRAIRLTPEDMPIDLIIHTPGGLALASEQIALALKEHKAKTTVIIPHYAMSGGSLIALAADEIIMDKNAVMGPVDPQIGQYPAASILEAYYRKGEKVSDETLILVDISKKAIKQMEEFVYELLKDKYGDEKAKEIAKKLTSGTWTHDYPLTVSKLKELGIEVNTNVPRKVYELLELYPQPMGAKPSVYYIPVPYSKKESEKNAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58891</accession>
    <name>Y1496_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0127 protein MJ1496</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1496</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0127 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99507" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99507.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64486">
        <property type="entry name" value="G64486"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58891"/>
    <dbReference type="SMR" id="Q58891"/>
    <dbReference type="STRING" id="243232.MJ_1496"/>
    <dbReference type="EnsemblBacteria" id="AAB99507">
        <property type="protein sequence ID" value="AAB99507"/>
        <property type="gene ID" value="MJ_1496"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1496"/>
    <dbReference type="eggNOG" id="arCOG03113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097039_4_2_2"/>
    <dbReference type="InParanoid" id="Q58891"/>
    <dbReference type="OMA" id="HIHTFFM"/>
    <dbReference type="PhylomeDB" id="Q58891"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00263">
        <property type="entry name" value="UPF0127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022906">
        <property type="entry name" value="UPF0127"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="PTHR37953"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150050" description="UPF0127 protein MJ1496">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="114" mass="13605" checksum="59FFDE4D35F0BE01" modified="2000-12-01" version="2">MQNKKIKKVKVGNLEFEVVLADNFIKRAFGLMLRDIGDKAMLFLYKRRKIAMHTFFMLYPIDIIFIDKNRVVEAVRLKPWKTYKCKNYSTAMLEFKCSDIDLNEIIGEKVEFMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58909</accession>
    <name>Y1514_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative gamma-glutamylcyclotransferase MJ1514</fullName>
            <ecNumber>2.3.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1514</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative gamma-glutamylcyclotransferase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64489">
        <property type="entry name" value="A64489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871037.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58909"/>
    <dbReference type="SMR" id="Q58909"/>
    <dbReference type="STRING" id="243232.MJ_1514"/>
    <dbReference type="EnsemblBacteria" id="AAB99532">
        <property type="protein sequence ID" value="AAB99532"/>
        <property type="gene ID" value="MJ_1514"/>
    </dbReference>
    <dbReference type="GeneID" id="1452421"/>
    <dbReference type="KEGG" id="mja:MJ_1514"/>
    <dbReference type="eggNOG" id="arCOG03271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083466_5_1_2"/>
    <dbReference type="InParanoid" id="Q58909"/>
    <dbReference type="OMA" id="KEKYAMY"/>
    <dbReference type="OrthoDB" id="114717at2157"/>
    <dbReference type="PhylomeDB" id="Q58909"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061929">
        <property type="term" value="F:gamma-glutamylaminecyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039126">
        <property type="entry name" value="GGACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12510">
        <property type="entry name" value="PTHR12510"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000184794" description="Putative gamma-glutamylcyclotransferase MJ1514">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="120" mass="14209" checksum="8AD873043D6ABEF3" modified="1996-11-01" version="1">MEYVFVYGSLRKGFWNHEPYLKNSKFIGKGKTKEKYAMYVNIIPYVVENEKISHIVGEVYEVDEKTLKRIDCLEGHPDYYRRKKVSIILDSGKEIEAWLYFYPESCGILVESGDYKDYRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58914</accession>
    <name>Y1519_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1519</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1519</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64489">
        <property type="entry name" value="F64489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871043.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58914"/>
    <dbReference type="STRING" id="243232.MJ_1519"/>
    <dbReference type="EnsemblBacteria" id="AAB99538">
        <property type="protein sequence ID" value="AAB99538"/>
        <property type="gene ID" value="MJ_1519"/>
    </dbReference>
    <dbReference type="GeneID" id="1452427"/>
    <dbReference type="KEGG" id="mja:MJ_1519"/>
    <dbReference type="eggNOG" id="arCOG00558">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006651_0_0_2"/>
    <dbReference type="InParanoid" id="Q58914"/>
    <dbReference type="OMA" id="FYWEHLG"/>
    <dbReference type="OrthoDB" id="43490at2157"/>
    <dbReference type="PhylomeDB" id="Q58914"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029493">
        <property type="entry name" value="RecD-like_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14490">
        <property type="entry name" value="HHH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107391" description="Uncharacterized protein MJ1519">
        <location>
            <begin position="1"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="586"/>
            <end position="593"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="1175" mass="138619" checksum="99082EA5A4D11140" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58918</accession>
    <name>CRCB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative fluoride ion transporter CrcB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">crcB</name>
        <name type="ordered locus">MJ1523</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CrcB (TC 9.B.71) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64490">
        <property type="entry name" value="B64490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871047.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58918"/>
    <dbReference type="SMR" id="Q58918"/>
    <dbReference type="STRING" id="243232.MJ_1523"/>
    <dbReference type="EnsemblBacteria" id="AAB99542">
        <property type="protein sequence ID" value="AAB99542"/>
        <property type="gene ID" value="MJ_1523"/>
    </dbReference>
    <dbReference type="GeneID" id="1452431"/>
    <dbReference type="KEGG" id="mja:MJ_1523"/>
    <dbReference type="eggNOG" id="arCOG04701">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114342_3_0_2"/>
    <dbReference type="InParanoid" id="Q58918"/>
    <dbReference type="OMA" id="FLMFESF"/>
    <dbReference type="OrthoDB" id="112898at2157"/>
    <dbReference type="PhylomeDB" id="Q58918"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903425">
        <property type="term" value="F:fluoride transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903424">
        <property type="term" value="P:fluoride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="CrcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="CrcB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="PTHR28259"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000110228" description="Putative fluoride ion transporter CrcB">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <sequence length="124" mass="13534" checksum="E5B702BC06104B9D" modified="1996-11-01" version="1">MIRELLLIGVGGFFGAIFRYLISGIVPVKFGLPTGTLAVNLIGSFILGFLLYCSLFAPIPTEYKLFIGTGFCGALTTFSTFSYETFVLVDEGLLFKALLNILINVVGCLIMVYFGRVLALAIFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58939</accession>
    <name>Y1544_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1544</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1544</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64492">
        <property type="entry name" value="G64492"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58939"/>
    <dbReference type="STRING" id="243232.MJ_1544"/>
    <dbReference type="EnsemblBacteria" id="AAB99562">
        <property type="protein sequence ID" value="AAB99562"/>
        <property type="gene ID" value="MJ_1544"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1544"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041527_0_0_2"/>
    <dbReference type="InParanoid" id="Q58939"/>
    <dbReference type="OMA" id="HLNDSYV"/>
    <dbReference type="PhylomeDB" id="Q58939"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025420">
        <property type="entry name" value="DUF4143"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13635">
        <property type="entry name" value="DUF4143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107400" description="Uncharacterized protein MJ1544">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="441" mass="52563" checksum="AA0B23729523BCE2" modified="1996-11-01" version="1">MSNILDIKILRVSSMIDKKILFEEVILDNLEIAKKAKVINRDIEIKLIPNKIKVIYGVRRGGKTYFLFQIINKHFKDDFIYINFEDERLINIALDELNELLKIALSIKNTKNLFFDEIQSVDNWDKFVRRLNDSGFNIFITGSSSKLLSKEIATSLRGRNLKTEILPLNFKEFLKFKNFNVKKRYSTIEKAELLKYLNEFIKFGGFPEITLIDDENIKKEILKEYLDGIFYRDVVERHSIRNIKEIKVLRNILINLFANEISIKKIANLLKEFNTKISRECIYNYLEYFSDAYLIFLLNNFSYKTKTISYSKLYVIDGMWNFSLSFSKNKGRILENLVFLELRRRGFVENENLFYVKRKNYEVDFLIFGENKELIQVCYELNETNKEREIKAYEKAIKDLKLDNVNLKIITYNDEGFEKITVDDKEHLIEIVPFWKWSLTY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58940</accession>
    <name>Y1545_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ1545</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1545</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64492">
        <property type="entry name" value="H64492"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58940"/>
    <dbReference type="SMR" id="Q58940"/>
    <dbReference type="STRING" id="243232.MJ_1545"/>
    <dbReference type="EnsemblBacteria" id="AAB99563">
        <property type="protein sequence ID" value="AAB99563"/>
        <property type="gene ID" value="MJ_1545"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1545"/>
    <dbReference type="eggNOG" id="arCOG04060">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077869_0_0_2"/>
    <dbReference type="InParanoid" id="Q58940"/>
    <dbReference type="OMA" id="KMAGEIT"/>
    <dbReference type="PhylomeDB" id="Q58940"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000149793" description="Uncharacterized HTH-type transcriptional regulator MJ1545">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="32"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00257"/>
        </source>
    </evidence>
    <sequence length="241" mass="27274" checksum="F4898E3F80842591" modified="1996-11-01" version="1">MTFYFYFVINMEKVAIYIIGDIVLAENTGKALKKWRNLFNIQQIELAKYLNVSPSVISDYEVGRRKNPGVNIIKKYVLALIEIDKEKGGQTIKALKRILDKSPSMKAILSIKEYENPITLNEFVNIIDGEIAVGDNSDTPIYGHTVVDSIKAILEMTGDDFYHLYGWTTERALIFTNVSTGRSPMVAVRVSIMKPRVVVLQGINKDKIDSLALKLAEIDNIPLITTHLDTKELIKRLNEIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58990</accession>
    <name>Y1595_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0104 membrane protein MJ1595</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1595</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0104 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64499">
        <property type="entry name" value="B64499"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58990"/>
    <dbReference type="STRING" id="243232.MJ_1595"/>
    <dbReference type="EnsemblBacteria" id="AAB99613">
        <property type="protein sequence ID" value="AAB99613"/>
        <property type="gene ID" value="MJ_1595"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1595"/>
    <dbReference type="eggNOG" id="arCOG00899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048072_1_1_2"/>
    <dbReference type="InParanoid" id="Q58990"/>
    <dbReference type="OMA" id="FINNVTP"/>
    <dbReference type="PhylomeDB" id="Q58990"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000138105" description="UPF0104 membrane protein MJ1595">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="336" mass="38163" checksum="282DC6E6C983198D" modified="1996-11-01" version="1">MMKIKITKSTILLIISFLFILAIMAYIGLDKIIKVLINTNPEYVILAFILQILVSVILSARWKFIIKILGYSANFKNIFLLVLMGLFINNITPSMRGGGEAFRAYYLSKLEEIPKGLAFSTVVVERVLDTAIFLFFTLFVIGYFVVTGFKYLEYLILSWIFLFSLTAIIIYLIANKGLLIKTVTKISKFICKYCSYNYDETKILQSIEEFYNSMKFFKNKRGWEVVVAIFLSVMRYIFDILKLWLLFLSLSYVVSVICVSAVYLITLLSGVLSITPSGFGTADTVMILSFSAFNIPPSVAAAVTLLDRLVSYILPTILGYIAMLIIKREIDKKKGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-11-02" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q59000</accession>
    <name>G6PI_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glucose-6-phosphate isomerase</fullName>
            <shortName evidence="1">GPI</shortName>
            <ecNumber evidence="1">5.3.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoglucose isomerase</fullName>
            <shortName evidence="1">PGI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphohexose isomerase</fullName>
            <shortName evidence="1">PHI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgi</name>
        <name type="ordered locus">MJ1605</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Arch. Microbiol." volume="181" first="82" last="87">
            <title>Glucose-6-phosphate isomerase from the hyperthermophilic archaeon Methanococcus jannaschii: characterization of the first archaeal member of the phosphoglucose isomerase superfamily.</title>
            <authorList>
                <person name="Rudolph B."/>
                <person name="Hansen T."/>
                <person name="Schonheit P."/>
            </authorList>
            <dbReference type="PubMed" id="14655001"/>
            <dbReference type="DOI" id="10.1007/s00203-003-0626-4"/>
        </citation>
        <scope>FUNCTION AS A GLUCOSE-6-PHOSPHATE ISOMERASE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="Biochemistry" volume="45" first="12366" last="12379">
            <title>Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="White R.H."/>
                <person name="Xu H."/>
            </authorList>
            <dbReference type="PubMed" id="17014089"/>
            <dbReference type="DOI" id="10.1021/bi061018a"/>
        </citation>
        <scope>FUNCTION AS A GLUCOSE-6-PHOSPHATE ISOMERASE</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the isomerization of glucose-6-P to fructose-6-P.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Competively inhibited by 6-phosphogluconate and erythrose 4-phosphate.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.04 mM for F6P (at pH 6.3 and 50 degrees Celsius)</KM>
            <KM evidence="2">1 mM for G6P (at pH 6.3 and 50 degrees Celsius)</KM>
            <Vmax evidence="2">9.0 umol/min/mg enzyme for G6P (at pH 6.3 and 50 degrees Celsius)</Vmax>
            <Vmax evidence="2">21.0 umol/min/mg enzyme for F6P (at pH 6.3 and 50 degrees Celsius)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 6.3. 50% remaining activity is observed at pH 5.3 and pH 7.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 89 degrees Celsius. It does not lose activity upon incubation at 80 degrees Celsius for about 120 min and still has a half-life at 95 degrees Celsius of 40 min. At 100 degrees Celsius, an almost complete loss of activity is observed after 30 min.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GPI family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64500">
        <property type="entry name" value="D64500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871130.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59000"/>
    <dbReference type="SMR" id="Q59000"/>
    <dbReference type="STRING" id="243232.MJ_1605"/>
    <dbReference type="EnsemblBacteria" id="AAB99624">
        <property type="protein sequence ID" value="AAB99624"/>
        <property type="gene ID" value="MJ_1605"/>
    </dbReference>
    <dbReference type="GeneID" id="1452514"/>
    <dbReference type="KEGG" id="mja:MJ_1605"/>
    <dbReference type="eggNOG" id="arCOG00052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037303_1_0_2"/>
    <dbReference type="InParanoid" id="Q59000"/>
    <dbReference type="OMA" id="CPAYAYG"/>
    <dbReference type="OrthoDB" id="24853at2157"/>
    <dbReference type="PhylomeDB" id="Q59000"/>
    <dbReference type="SABIO-RK" id="Q59000"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05016">
        <property type="entry name" value="SIS_PGI_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00473">
        <property type="entry name" value="G6P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035482">
        <property type="entry name" value="SIS_PGI_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="PTHR11469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000180780" description="Glucose-6-phosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="282"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="14655001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17014089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="401" mass="45675" checksum="D5B766A9F2230ED9" modified="1996-11-01" version="1">MLSYDYENALKVGEISLEDINKVDFANAYSNLMEKLDNGVVGFRDVIYDENLDKYKSLNGYENVVVIGMGGSILGTMAIYYAISPFNNNAYFIDNSDPEKTLSILKKVDLNESIIYIISKSGNTLETLVNYYLIKKRIEKLNSFKGKLVFITNGGKLKREAEKNNYDIFSIPENVPGRFSVFTAVGLAPLYSLGVDISKILEGAREMDKICQNEDILKNPALLNGVIHYLYDKRGKDISVIMSYVESLKYFGDWYKQLIGESLGKNKHGITPLLSIGAKDQHSLLQLYMDGKKDKIITFMVAKKYRLDEEIEFEDINDEKISCRYSDIIRSQQKATEIALTNNGVPNVRITLDEINEMAMGALLYMYEMQVGFMGELYNINAYNQPAVEEEKKICWRLIKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q59003</accession>
    <name>Y1608_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1608</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1608</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64500">
        <property type="entry name" value="G64500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871133.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59003"/>
    <dbReference type="SMR" id="Q59003"/>
    <dbReference type="STRING" id="243232.MJ_1608"/>
    <dbReference type="EnsemblBacteria" id="AAB99635">
        <property type="protein sequence ID" value="AAB99635"/>
        <property type="gene ID" value="MJ_1608"/>
    </dbReference>
    <dbReference type="GeneID" id="1452517"/>
    <dbReference type="KEGG" id="mja:MJ_1608"/>
    <dbReference type="eggNOG" id="arCOG05093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_175324_2_1_2"/>
    <dbReference type="InParanoid" id="Q59003"/>
    <dbReference type="OMA" id="KMAIGWL"/>
    <dbReference type="OrthoDB" id="121606at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019707">
        <property type="entry name" value="DUF2582"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10771">
        <property type="entry name" value="DUF2582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107435" description="Uncharacterized protein MJ1608">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <sequence length="70" mass="8122" checksum="BDC6BE5A1A47AF57" modified="1996-11-01" version="1">MEDMWGKIGETAGKIYHLLEGGEKSLSQIEKILRKEGYNSNIVKMAIGWLAREDKIFVLKDDKKWVIKLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q59012</accession>
    <name>Y1617_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1617</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1617</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64501">
        <property type="entry name" value="H64501"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59012"/>
    <dbReference type="STRING" id="243232.MJ_1617"/>
    <dbReference type="EnsemblBacteria" id="AAB99646">
        <property type="protein sequence ID" value="AAB99646"/>
        <property type="gene ID" value="MJ_1617"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1617"/>
    <dbReference type="eggNOG" id="arCOG02717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149108_3_0_2"/>
    <dbReference type="InParanoid" id="Q59012"/>
    <dbReference type="OMA" id="PEITTNM"/>
    <dbReference type="PhylomeDB" id="Q59012"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00304">
        <property type="entry name" value="TIGR00304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107439" description="Uncharacterized protein MJ1617">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="93" mass="10461" checksum="589E72AC270850A9" modified="1996-11-01" version="1">MIMKPILIFLGIILMFIGFFMITLGMILPSSQENYEKPETEKTESSVEYSGIVMIGPIPIVFGNSPGLMILSVLIAILMIIWMFLFAFGIRIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q59017</accession>
    <name>Y1623_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1623</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1623</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64502">
        <property type="entry name" value="E64502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871147.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59017"/>
    <dbReference type="SMR" id="Q59017"/>
    <dbReference type="STRING" id="243232.MJ_1623"/>
    <dbReference type="EnsemblBacteria" id="AAB99648">
        <property type="protein sequence ID" value="AAB99648"/>
        <property type="gene ID" value="MJ_1623"/>
    </dbReference>
    <dbReference type="GeneID" id="1452532"/>
    <dbReference type="KEGG" id="mja:MJ_1623"/>
    <dbReference type="eggNOG" id="arCOG02037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG02838">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_523401_0_0_2"/>
    <dbReference type="InParanoid" id="Q59017"/>
    <dbReference type="OMA" id="GFVFFDW"/>
    <dbReference type="OrthoDB" id="13479at2157"/>
    <dbReference type="PhylomeDB" id="Q59017"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10442">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107444" description="Uncharacterized protein MJ1623">
        <location>
            <begin position="1"/>
            <end position="512"/>
        </location>
    </feature>
    <sequence length="512" mass="59701" checksum="78172A3E6C9396E4" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q59021</accession>
    <name>Y1627_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0179 protein MJ1627</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1627</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0179 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64503">
        <property type="entry name" value="A64503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871151.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59021"/>
    <dbReference type="STRING" id="243232.MJ_1627"/>
    <dbReference type="EnsemblBacteria" id="AAB99644">
        <property type="protein sequence ID" value="AAB99644"/>
        <property type="gene ID" value="MJ_1627"/>
    </dbReference>
    <dbReference type="GeneID" id="1452536"/>
    <dbReference type="KEGG" id="mja:MJ_1627"/>
    <dbReference type="eggNOG" id="arCOG04477">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121764_0_0_2"/>
    <dbReference type="InParanoid" id="Q59021"/>
    <dbReference type="OMA" id="CPSHEYC"/>
    <dbReference type="OrthoDB" id="108341at2157"/>
    <dbReference type="PhylomeDB" id="Q59021"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00498">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005369">
        <property type="entry name" value="UPF0179"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699">
        <property type="entry name" value="PTHR40699"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03684">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006595">
        <property type="entry name" value="UCP006595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156870" description="UPF0179 protein MJ1627">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="151" mass="16941" checksum="2E18EB717F4E779E" modified="1996-11-01" version="1">MSKESKITLIGSKLAKTGGEFIYLGEIEECKNCKFKRLCHGNLEVGRKYKIVSVRSANHPCIVHEGGVKVVEVVLADLTIMIESKKALEGVVLNHEPITCDNFDCEYYSFCNSEGIKEGEKYKIKQVLNEKINCPFGNSLKKVIVELVENK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q59058</accession>
    <name>Y1664_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1664</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1664</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64507">
        <property type="entry name" value="F64507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871188.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59058"/>
    <dbReference type="SMR" id="Q59058"/>
    <dbReference type="STRING" id="243232.MJ_1664"/>
    <dbReference type="EnsemblBacteria" id="AAB99682">
        <property type="protein sequence ID" value="AAB99682"/>
        <property type="gene ID" value="MJ_1664"/>
    </dbReference>
    <dbReference type="GeneID" id="1452573"/>
    <dbReference type="KEGG" id="mja:MJ_1664"/>
    <dbReference type="eggNOG" id="arCOG09628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1222531_0_0_2"/>
    <dbReference type="OMA" id="IFECKNR"/>
    <dbReference type="OrthoDB" id="79871at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107462" description="Uncharacterized protein MJ1664">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <sequence length="226" mass="26892" checksum="7154EC06DBDF94C9" modified="1996-11-01" version="1">MNKLKLKNEKEVRIKFGEYKLTGFSSDNGNNLYKLFNLTLSKIRYRKAMLEHNFQLPKIKESHKPKEITEKIKENDIYFLELINEIKKDFKDFCSLDAGTLFELFMYYTLIEFFKENNIDAKVIRNLDVSYKGNIFTEIDLFVDVFGKNFIFECKNRHISSNAILKLYGIMKILNINFGVLASTKGFYGNLKKEDIFKEYNIYILDKLIEKEKNKIFKELKDIFNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q59060</accession>
    <name>CSX1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system endoribonuclease Csx1</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">csx1</name>
        <name type="ordered locus">MJ1666</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Activated by cyclic oligoadenylate (cOA) to endonucleolytically degrade linear RNA; when cOA levels decrease the RNase activity of Csx1 is abrogated.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The RNase activity is stimulated by cOA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Encoded in a type III-A CRISPR locus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csx1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64507">
        <property type="entry name" value="H64507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871190.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59060"/>
    <dbReference type="SMR" id="Q59060"/>
    <dbReference type="STRING" id="243232.MJ_1666"/>
    <dbReference type="PRIDE" id="Q59060"/>
    <dbReference type="EnsemblBacteria" id="AAB99691">
        <property type="protein sequence ID" value="AAB99691"/>
        <property type="gene ID" value="MJ_1666"/>
    </dbReference>
    <dbReference type="GeneID" id="1452575"/>
    <dbReference type="KEGG" id="mja:MJ_1666"/>
    <dbReference type="eggNOG" id="arCOG03433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044033_0_0_2"/>
    <dbReference type="InParanoid" id="Q59060"/>
    <dbReference type="OMA" id="ILIAPWG"/>
    <dbReference type="OrthoDB" id="110543at2157"/>
    <dbReference type="PhylomeDB" id="Q59060"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013383">
        <property type="entry name" value="CRISPR-assoc_prot_DxTHG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010171">
        <property type="entry name" value="CRISPR_Csx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019016">
        <property type="entry name" value="Csx1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09455">
        <property type="entry name" value="Cas_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01897">
        <property type="entry name" value="cas_MJ1666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02549">
        <property type="entry name" value="CRISPR_DxTHG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107463" description="CRISPR system endoribonuclease Csx1">
        <location>
            <begin position="1"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q97YD5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="465" mass="54500" checksum="ACB9E442EEF94173" modified="1996-11-01" version="1">MQKILIAPWGNFSSWKKVIYSFNGVEKESKSSLSAIYDKINPDKVYILVLDTLSNLESENYGDIVKEVKEKTENFIKENLNIDNYEVIVCPGVGTFYNKDFEKYFKFYGNLTDYYSFALYELSKRLDGDLEVHLDLTHGLNYMPVLTYRVIKDLLEILAIKNKVRLVVYNSDPYVGREKEILNIHTVEDVIIKPSYDIKGMTLDFLDATKFVDKKEIGKIKKEINMNPKIKELRIMKQNINAFIASIVYALPLVYSTFFVKKDKIEIYLNELIGAFISNIKINPEDKILKRYLYFGEGFNSLVKAYFASKISEIPQLIKDELSLEEIDELKNTLFKENPNSQYIKNEISSLYNIINTKYKEEELSEILGNWTPIYKIRRENIDKFKIRNFLAHAGFEKSVTEIYISVENKNGKIELSEKTSLRYNKNYIEEKNGIKRFIFKYKDKNGKVEEINILEKIEEILLNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q59069</accession>
    <name>TRUA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="1">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truA</name>
        <name type="ordered locus">MJ1675</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64509">
        <property type="entry name" value="A64509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871199.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59069"/>
    <dbReference type="SMR" id="Q59069"/>
    <dbReference type="STRING" id="243232.MJ_1675"/>
    <dbReference type="PRIDE" id="Q59069"/>
    <dbReference type="EnsemblBacteria" id="AAB99697">
        <property type="protein sequence ID" value="AAB99697"/>
        <property type="gene ID" value="MJ_1675"/>
    </dbReference>
    <dbReference type="GeneID" id="1452584"/>
    <dbReference type="KEGG" id="mja:MJ_1675"/>
    <dbReference type="eggNOG" id="arCOG04449">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014673_4_2_2"/>
    <dbReference type="InParanoid" id="Q59069"/>
    <dbReference type="OMA" id="MYIFKIA"/>
    <dbReference type="OrthoDB" id="62389at2157"/>
    <dbReference type="PhylomeDB" id="Q59069"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PTHR11142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000057503" description="tRNA pseudouridine synthase A">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <sequence length="259" mass="30110" checksum="3B6A8C4AF03A5C85" modified="1996-11-01" version="1">MYILKIAYDGRYSFQQQPHKQTVCDILLDALEETGFLAKKKVIYSGGRTDKGVSALGNFVVVELKKEPILSYINAKLKDKGIWVLGYREIDEIPKVKYRHYRYILPNIGYDVDAIKEGAKKMIGTHSFHNLSKRDRTKEKSPIRTIYDIKISENEFYLTVDIIGESFLWNMVRKIVGALDLVGRGKKPVEWIDKLLDENHREGVPTFPPEGLILVEAKVDIEYIYDNYSIRKFKEYWGEFFKLQVMKIGIAKTVLEFTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q59071</accession>
    <name>Y1677_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0056 membrane protein MJ1677</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1677</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64509">
        <property type="entry name" value="C64509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871201.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59071"/>
    <dbReference type="STRING" id="243232.MJ_1677"/>
    <dbReference type="EnsemblBacteria" id="AAB99699">
        <property type="protein sequence ID" value="AAB99699"/>
        <property type="gene ID" value="MJ_1677"/>
    </dbReference>
    <dbReference type="GeneID" id="1452586"/>
    <dbReference type="KEGG" id="mja:MJ_1677"/>
    <dbReference type="eggNOG" id="arCOG01997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079909_1_0_2"/>
    <dbReference type="InParanoid" id="Q59071"/>
    <dbReference type="OMA" id="LFVIMDP"/>
    <dbReference type="OrthoDB" id="88537at2157"/>
    <dbReference type="PhylomeDB" id="Q59071"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="PTHR33508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00427">
        <property type="entry name" value="TIGR00427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000156918" description="UPF0056 membrane protein MJ1677">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="205" mass="22304" checksum="3C68D938EFA689AC" modified="1996-11-01" version="1">MDLQYFILAFSSIFSILNPFGAVPVFITLTESYPKKERDLVAKKTVIYALAILLAFALFGEWILKFFGISLDAFKIAGGILLLLISLDMVRGQQEAKIHRKEIEAAYEIDEIALMPLATPLLAGPGSITACMVAMAEASDIGDKFLVILAILLSLGITYLTLLSAESVLDRIGRLGIRILTRMMGLILTAIAVQMIVNGIRGALL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q60181</accession>
    <name>RPO2C_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo2C</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit B'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo2C</name>
        <name type="synonym">rpoB1</name>
        <name type="ordered locus">MJ1041</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this organism) is implicated in DNA promoter recognition and in nucleotide binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64429">
        <property type="entry name" value="H64429"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60181"/>
    <dbReference type="SMR" id="Q60181"/>
    <dbReference type="STRING" id="243232.MJ_1041"/>
    <dbReference type="EnsemblBacteria" id="AAB99044">
        <property type="protein sequence ID" value="AAB99044"/>
        <property type="gene ID" value="MJ_1041"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1041"/>
    <dbReference type="eggNOG" id="arCOG01762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_2_2_2"/>
    <dbReference type="InParanoid" id="Q60181"/>
    <dbReference type="OMA" id="YQKLYHM"/>
    <dbReference type="PhylomeDB" id="Q60181"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019969">
        <property type="entry name" value="RNAP_Rpo2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03670">
        <property type="entry name" value="rpoB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000048102" description="DNA-directed RNA polymerase subunit Rpo2C">
        <location>
            <begin position="1"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="554"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="569"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="572"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="B8YB55"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P11513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="8688087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="636" mass="72193" checksum="81DE30267A572914" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q60262</accession>
    <name>Y3505_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL05</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL05</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64510">
        <property type="entry name" value="E64510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890052.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60262"/>
    <dbReference type="SMR" id="Q60262"/>
    <dbReference type="STRING" id="243232.MJ_ECL05"/>
    <dbReference type="EnsemblBacteria" id="AAC37071">
        <property type="protein sequence ID" value="AAC37071"/>
        <property type="gene ID" value="MJ_ECL05"/>
    </dbReference>
    <dbReference type="GeneID" id="1450784"/>
    <dbReference type="KEGG" id="mja:MJ_ECL05"/>
    <dbReference type="eggNOG" id="arCOG10969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_210692_0_0_2"/>
    <dbReference type="OMA" id="GMDWKEI"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107497" description="Uncharacterized protein MJECL05">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <sequence length="62" mass="7327" checksum="1624EC72E75EBAD7" modified="1997-11-01" version="1">MEIIALLIEEGIIIIKDKKVAERFLKDLESSQGMDWKEIRERAERAKKQLEEGIEWAKKTKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q60266</accession>
    <name>Y3501_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL01</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL01</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64510">
        <property type="entry name" value="A64510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890048.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60266"/>
    <dbReference type="STRING" id="243232.MJ_ECL01"/>
    <dbReference type="EnsemblBacteria" id="AAC37075">
        <property type="protein sequence ID" value="AAC37075"/>
        <property type="gene ID" value="MJ_ECL01"/>
    </dbReference>
    <dbReference type="GeneID" id="1450788"/>
    <dbReference type="KEGG" id="mja:MJ_ECL01"/>
    <dbReference type="eggNOG" id="arCOG00877">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1113870_0_0_2"/>
    <dbReference type="OrthoDB" id="8192at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107493" description="Uncharacterized protein MJECL01">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <sequence length="249" mass="29998" checksum="5420C6442CB84E31" modified="1997-11-01" version="1">MMGEQLDYLLNIVKNDKKFLETLKNMKIDVDSDEELSKIFFIIITFPFWLRCHDDIKNALRLIDKITNKGSFTKVLDNSMKEYHVFYIYDVDKLSDLIAEELKRLYLICKSEGKSYYENIFFRLDRKSILKLFMKISPFKQLDVIKGKLKNQLDFFEEYFNNIEKIGYFSIRMENSRYNEHIKSLHPKLIISLRVDKVHIRLEAQIDYYNINNENEEAYKKILELMKLNLLTIGYKAVEKFLEEIYEEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q60298</accession>
    <name>Y3543_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL43</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64515">
        <property type="entry name" value="B64515"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890091.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60298"/>
    <dbReference type="STRING" id="243232.MJ_ECL43"/>
    <dbReference type="PRIDE" id="Q60298"/>
    <dbReference type="EnsemblBacteria" id="AAC37112">
        <property type="protein sequence ID" value="AAC37112"/>
        <property type="gene ID" value="MJ_ECL43"/>
    </dbReference>
    <dbReference type="GeneID" id="1450825"/>
    <dbReference type="KEGG" id="mja:MJ_ECL43"/>
    <dbReference type="eggNOG" id="arCOG03235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718892_0_0_2"/>
    <dbReference type="OrthoDB" id="23345at2157"/>
    <dbReference type="PhylomeDB" id="Q60298"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107522" description="Uncharacterized protein MJECL43">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <sequence length="384" mass="45886" checksum="DA032E83C2433D2E" modified="1997-11-01" version="1">MNIDIEDFEKKPLYIQRIILRNSCIEFSKMLKKYWDKNIMIGIQISEREMSFEIEERDNKNEPIKITLPEYRSKGFKWYLAYLITLEYLRILKNGGNNDIVLLLDDPAVYLHPNVQKSFLEKLEELSKEYQILYNTHLMSLFNEEELDRVLLVYLDKENRTKIKRPWSNEQKDIIYPIRRALGVDKILFKENLSKILFVEGISDKFILEGLGKLETLKNLKNWYIHPLSGGDKLEDNEIVKKVRLYSCLSNFEEIKYYFLLDGDKKEKFERDKISNKIIFLGDENQEIEDLIDKNFYLDCVLETYKRIFTHDLEKFKKVKEIVEKLRKSKSKIIEELNNEFRLNNLGDFSKVDVAITIKRKLYENPELANKFEKIIDCLNGKII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q60329</accession>
    <name>Y014_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0014</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0014</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64301">
        <property type="entry name" value="F64301"/>
    </dbReference>
    <dbReference type="PDB" id="3LHK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B/C/D=63-213"/>
    </dbReference>
    <dbReference type="PDBsum" id="3LHK"/>
    <dbReference type="AlphaFoldDB" id="Q60329"/>
    <dbReference type="SMR" id="Q60329"/>
    <dbReference type="STRING" id="243232.MJ_0014"/>
    <dbReference type="EnsemblBacteria" id="AAB97992">
        <property type="protein sequence ID" value="AAB97992"/>
        <property type="gene ID" value="MJ_0014"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0014"/>
    <dbReference type="eggNOG" id="arCOG03164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082093_0_1_2"/>
    <dbReference type="InParanoid" id="Q60329"/>
    <dbReference type="OMA" id="RFIRFGY"/>
    <dbReference type="PhylomeDB" id="Q60329"/>
    <dbReference type="EvolutionaryTrace" id="Q60329"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03769">
        <property type="entry name" value="SR_IS607_transposase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041657">
        <property type="entry name" value="HTH_17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041718">
        <property type="entry name" value="IS607_transposase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006119">
        <property type="entry name" value="Resolv_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036162">
        <property type="entry name" value="Resolvase-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010093">
        <property type="entry name" value="SinI_DNA-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12728">
        <property type="entry name" value="HTH_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00239">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00857">
        <property type="entry name" value="Resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53041">
        <property type="entry name" value="SSF53041"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01764">
        <property type="entry name" value="excise"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51736">
        <property type="entry name" value="RECOMBINASES_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106654" description="Uncharacterized protein MJ0014">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Resolvase/invertase-type recombinase catalytic" evidence="1">
        <location>
            <begin position="63"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-serine intermediate" evidence="1">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="73"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="98"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="126"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="155"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="167"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="191"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3LHK"/>
        </source>
    </evidence>
    <sequence length="213" mass="25041" checksum="7786C8202AA34898" modified="1997-11-01" version="1">MMIMERHYTLKEASKILGVSIKTLQRWDKAGKIKCIRTLGGKRRVPESEIKRILGIKDKEQRKIIGYARVSFNAQKDDLERQIQLIKSYAEENGWDIQILKDIGSGLNEKRKNYKKLLKMVMNRKVEKVIIAYPDRLTRFGFETLKEFFKSYGTEIVIINKKHKTPQEELVEDLITIVSHFAGKLYGMHSHKYKKLTKTVKEIVREEDAKEKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q60335</accession>
    <name>RTCA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>RNA 3'-terminal phosphate cyclase</fullName>
            <shortName>RNA cyclase</shortName>
            <shortName>RNA-3'-phosphate cyclase</shortName>
            <ecNumber>6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rtcA</name>
        <name type="ordered locus">MJ0025</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64303">
        <property type="entry name" value="A64303"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869517.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60335"/>
    <dbReference type="SMR" id="Q60335"/>
    <dbReference type="STRING" id="243232.MJ_0025"/>
    <dbReference type="EnsemblBacteria" id="AAB98006">
        <property type="protein sequence ID" value="AAB98006"/>
        <property type="gene ID" value="MJ_0025"/>
    </dbReference>
    <dbReference type="GeneID" id="1450863"/>
    <dbReference type="KEGG" id="mja:MJ_0025"/>
    <dbReference type="eggNOG" id="arCOG04125">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027882_0_0_2"/>
    <dbReference type="InParanoid" id="Q60335"/>
    <dbReference type="OMA" id="PKPGLSH"/>
    <dbReference type="OrthoDB" id="52581at2157"/>
    <dbReference type="PhylomeDB" id="Q60335"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="PTHR11096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="PTHR11096:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52913">
        <property type="entry name" value="SSF52913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156426" description="RNA 3'-terminal phosphate cyclase">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="304"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="280"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="338" mass="37229" checksum="4681A2AB120FD6F0" modified="1997-11-01" version="1">MDFIVIDGSYLEGGGQIIRTAVSLSALTQKPVKIINIRKKRKNKGLAPQHVSAVKAVKKLCNAEVFGLNVGSEELTFIPSKLSPKDFTIDIGTAGSISLVIQTLLPLSLGINKKFTVKIKGGTDVKRAPPIDYVKNVTLKILRNFGVLTELKVLKRGFYPEGGGEVIFEVKPSKIKKFDLIEHSKSNLVEGISYVQNLDESIARRMRKKAVDLLNKEKLLPNIKIECSKGISTGAGIVLWNDTLGGSCLGEKGLRAEIVAERAVNELLKERESGMALDKYMGDQIIPFLAFGKGIVGVSEITNHTKTNMWVVKHFLDVDFEIKEYKENNCNGFTIEVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q60346</accession>
    <name>PUS10_METJA</name>
    <protein>
        <recommendedName>
            <fullName>tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="2">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>tRNA pseudouridine 54/55 synthase</fullName>
            <shortName>Psi54/55 synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pus10</name>
        <name type="ordered locus">MJ0041</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="RNA" volume="14" first="2521" last="2527">
            <title>Archaeal Pus10 proteins can produce both pseudouridine 54 and 55 in tRNA.</title>
            <authorList>
                <person name="Gurha P."/>
                <person name="Gupta R."/>
            </authorList>
            <dbReference type="PubMed" id="18952823"/>
            <dbReference type="DOI" id="10.1261/rna.1276508"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Responsible for synthesis of pseudouridine from uracil-54 and uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64305">
        <property type="entry name" value="A64305"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60346"/>
    <dbReference type="SMR" id="Q60346"/>
    <dbReference type="STRING" id="243232.MJ_0041"/>
    <dbReference type="EnsemblBacteria" id="AAB98022">
        <property type="protein sequence ID" value="AAB98022"/>
        <property type="gene ID" value="MJ_0041"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0041"/>
    <dbReference type="eggNOG" id="arCOG01015">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028780_2_0_2"/>
    <dbReference type="InParanoid" id="Q60346"/>
    <dbReference type="OMA" id="DGGLYIK"/>
    <dbReference type="PhylomeDB" id="Q60346"/>
    <dbReference type="BRENDA" id="5.4.99.25">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="5.4.99.B22">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="5.4.99.B25">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01893">
        <property type="entry name" value="Pus10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005912">
        <property type="entry name" value="Pus10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="PTHR21568"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000106664" description="tRNA pseudouridine synthase Pus10">
        <location>
            <begin position="1"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="THUMP">
        <location>
            <begin position="100"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18952823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="454" mass="52927" checksum="C91F61339D284209" modified="1997-11-01" version="1">MASIINKQIISPTGRNMEIINYEILKKYPLCDRCFGRLYAKLLHTTNTERGRALKLYKALELEAKIKKAKEKGINYEEELELLKALAKSGVDEIRLEDIEIEKENCPWCRGIFNKQKMEKLLNKAIELLKEYDFDTFLIGTHIPEEIKDLEKEIETEFMESIKQEFGREFGKMLAVRLDKAPDKEYPDIVVHINPYTEEIYLQINPLFIKGRYRKLVRGIPQTRWPCRKCRGKGCELCNYTGKKYPISVEEIIAKPFLEATKGVDAKFHGAGREDIDVRMLGDGRPFVLEIKEPKIRKIDLNKIAEEINKDGRVEVLNLEFGVRKDKVIFKNTPHRKTYRALVECSDKITDEELKLLEKELENRTIYQKTPKRVLHRRADLERIRKVYKVKTSKVDDNHFEMIIYCDGGLYIKELISGDDGRTNPSVSSILNKNCICKELDVLKIHDNNLLEKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q60383</accession>
    <name>Y076_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0076</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0076</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64309">
        <property type="entry name" value="E64309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869569.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60383"/>
    <dbReference type="STRING" id="243232.MJ_0076"/>
    <dbReference type="EnsemblBacteria" id="AAB98057">
        <property type="protein sequence ID" value="AAB98057"/>
        <property type="gene ID" value="MJ_0076"/>
    </dbReference>
    <dbReference type="GeneID" id="1450916"/>
    <dbReference type="KEGG" id="mja:MJ_0076"/>
    <dbReference type="eggNOG" id="arCOG08279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079438_0_0_2"/>
    <dbReference type="OMA" id="QFRIEEN"/>
    <dbReference type="OrthoDB" id="116306at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106682" description="Uncharacterized protein MJ0076">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <sequence length="113" mass="12949" checksum="1669DA93CD1F2F5C" modified="1997-11-01" version="1">MEVNLLSISIKPPIKIPKNKTVELDVDWNIEYKKLDAKKFNFVCNIKAYGDFSFEANIEGEISTENDYDNIPDFISVSIVENLMKSLPKLVSYAQQFRIEENVFVNQPLSLAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2022-05-25" version="13" xmlns="http://uniprot.org/uniprot">
    <accession>P0CW37</accession>
    <name>VAPB1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative antitoxin VapB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vapB1</name>
        <name type="ordered locus">MJ0913.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possibly the antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is VapC1.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496664.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P0CW37"/>
    <dbReference type="GeneID" id="27929980"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000408087" description="Putative antitoxin VapB1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="64" mass="7775" checksum="6E1AF77F98D8FCF1" modified="2011-05-03" version="1">MISAKSKTKRITITFEIPEDIDAKKFKDDVKRYVRYKLLANKLYELLEGENIEEIEEEIRKRRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1995-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>P43409</accession>
    <name>KADA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Adenylate kinase</fullName>
            <shortName>AK</shortName>
            <ecNumber>2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">adkA</name>
        <name type="synonym">adk</name>
        <name type="ordered locus">MJ0479</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1997" name="Gene" volume="185" first="239" last="244">
            <title>The adenylate kinase genes of M. voltae, M. thermolithotrophicus, M. jannaschii, and M. igneus define a new family of adenylate kinases.</title>
            <authorList>
                <person name="Ferber D.M."/>
                <person name="Haney P.J."/>
                <person name="Berk H."/>
                <person name="Lynn D."/>
                <person name="Konisky J."/>
            </authorList>
            <dbReference type="PubMed" id="9055821"/>
            <dbReference type="DOI" id="10.1016/S0378-1119(96)00651-8"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1995" name="J. Bacteriol." volume="177" first="2977" last="2981">
            <title>The adenylate kinases from a mesophilic and three thermophilic methanogenic members of the Archaea.</title>
            <authorList>
                <person name="Rusnak P."/>
                <person name="Haney P."/>
                <person name="Konisky J."/>
            </authorList>
            <dbReference type="PubMed" id="7768791"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-30</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text>Active from 70 to 90 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal adenylate kinase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98470" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.4.3"/>
    <dbReference type="EMBL" id="U39882">
        <property type="protein sequence ID" value="AAC44863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98470.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64359">
        <property type="entry name" value="G64359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496506.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P43409"/>
    <dbReference type="SMR" id="P43409"/>
    <dbReference type="STRING" id="243232.MJ_0479"/>
    <dbReference type="EnsemblBacteria" id="AAB98470">
        <property type="protein sequence ID" value="AAB98470"/>
        <property type="gene ID" value="MJ_0479"/>
    </dbReference>
    <dbReference type="GeneID" id="1451341"/>
    <dbReference type="KEGG" id="mja:MJ_0479"/>
    <dbReference type="eggNOG" id="arCOG01039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119371_0_0_2"/>
    <dbReference type="InParanoid" id="P43409"/>
    <dbReference type="OMA" id="HRDEMRK"/>
    <dbReference type="OrthoDB" id="79684at2157"/>
    <dbReference type="PhylomeDB" id="P43409"/>
    <dbReference type="BRENDA" id="2.7.4.3">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00234">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023477">
        <property type="entry name" value="Adenylate_kinase_AdkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000131814" description="Adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="sequence conflict" description="In Ref. 3; AA sequence." evidence="2" ref="3">
        <original>S</original>
        <variation>G</variation>
        <location>
            <position position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="192" mass="21772" checksum="ECD533AD4C85D99E" modified="1996-10-01" version="2">MKNKVVVIVGVPGVGSTTVTNKAIEELKKEGIEYKIVNFGTVMFEIAKEEGLVEHRDQLRKLPPEEQKRIQKLAGKKIAEMAKEFNIVVDTHSTIKTPKGYLPGLPAWVLEELNPDIIVLVEAENDEILMRRLKDETRQRDFESTEDIGEHIFMNRCAAMTYAVLTGATVKIIKNRDFLLDKAVQELIEVLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>P60460</accession>
    <name>SECG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Preprotein translocase subunit SecG</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Protein transport protein Sec61 subunit beta homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">secG</name>
        <name type="ordered locus">MJ0912.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Nature" volume="427" first="36" last="44">
            <title>X-ray structure of a protein-conducting channel.</title>
            <authorList>
                <person name="Van den Berg B."/>
                <person name="Clemons W.M. Jr."/>
                <person name="Collinson I."/>
                <person name="Modis Y."/>
                <person name="Hartmann E."/>
                <person name="Harrison S.C."/>
                <person name="Rapoport T.A."/>
            </authorList>
            <dbReference type="PubMed" id="14661030"/>
            <dbReference type="DOI" id="10.1038/nature02218"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 21-52 IN COMPLEX WITH SECY AND SECE</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2007" name="Mol. Cell" volume="26" first="511" last="521">
            <title>The plug domain of the SecY protein stabilizes the closed state of the translocation channel and maintains a membrane seal.</title>
            <authorList>
                <person name="Li W."/>
                <person name="Schulman S."/>
                <person name="Boyd D."/>
                <person name="Erlandson K."/>
                <person name="Beckwith J."/>
                <person name="Rapoport T.A."/>
            </authorList>
            <dbReference type="PubMed" id="17531810"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2007.05.002"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS)</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Mol. Cell" volume="28" first="1083" last="1092">
            <title>Ribosome binding of a single copy of the SecY complex: implications for protein translocation.</title>
            <authorList>
                <person name="Menetret J.F."/>
                <person name="Schaletzky J."/>
                <person name="Clemons W.M. Jr."/>
                <person name="Osborne A.R."/>
                <person name="Skanland S.S."/>
                <person name="Denison C."/>
                <person name="Gygi S.P."/>
                <person name="Kirkpatrick D.S."/>
                <person name="Park E."/>
                <person name="Ludtke S.J."/>
                <person name="Rapoport T.A."/>
                <person name="Akey C.W."/>
            </authorList>
            <dbReference type="PubMed" id="18158904"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2007.10.034"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (9.6 ANGSTROMS) OF 21-52 DOCKED ONTO E.COLI RIBOSOMES</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2008" name="Structure" volume="16" first="1126" last="1137">
            <title>Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome.</title>
            <authorList>
                <person name="Menetret J.F."/>
                <person name="Hegde R.S."/>
                <person name="Aguiar M."/>
                <person name="Gygi S.P."/>
                <person name="Park E."/>
                <person name="Rapoport T.A."/>
                <person name="Akey C.W."/>
            </authorList>
            <dbReference type="PubMed" id="18611385"/>
            <dbReference type="DOI" id="10.1016/j.str.2008.05.003"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (8.7 ANGSTROMS) OF 21-52 DOCKED ONTO DOG RIBOSOMES</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2009" name="Structure" volume="17" first="1453" last="1464">
            <title>Regulation of the protein-conducting channel by a bound ribosome.</title>
            <authorList>
                <person name="Gumbart J."/>
                <person name="Trabuco L.G."/>
                <person name="Schreiner E."/>
                <person name="Villa E."/>
                <person name="Schulten K."/>
            </authorList>
            <dbReference type="PubMed" id="19913480"/>
            <dbReference type="DOI" id="10.1016/j.str.2009.09.010"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY DOCKED ONTO E.COLI RIBOSOMES</scope>
    </reference>
    <comment type="function">
        <text>Subunit of the protein translocation channel SecYEG. The function of the beta subunit is unknown, but it may be involved in stabilization of the trimeric complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cell membrane</location>
            <topology>Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496662.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1RH5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="C=1-53"/>
    </dbReference>
    <dbReference type="PDB" id="1RHZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="C=1-53"/>
    </dbReference>
    <dbReference type="PDB" id="2YXQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="C=1-53"/>
    </dbReference>
    <dbReference type="PDB" id="2YXR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.60 A"/>
        <property type="chains" value="C=1-53"/>
    </dbReference>
    <dbReference type="PDB" id="3BO0">
        <property type="method" value="EM"/>
        <property type="resolution" value="9.60 A"/>
        <property type="chains" value="C=21-52"/>
    </dbReference>
    <dbReference type="PDB" id="3BO1">
        <property type="method" value="EM"/>
        <property type="resolution" value="9.60 A"/>
        <property type="chains" value="C=21-52"/>
    </dbReference>
    <dbReference type="PDB" id="3DKN">
        <property type="method" value="EM"/>
        <property type="resolution" value="8.70 A"/>
        <property type="chains" value="C=21-52"/>
    </dbReference>
    <dbReference type="PDB" id="4V4N">
        <property type="method" value="EM"/>
        <property type="resolution" value="9.00 A"/>
        <property type="chains" value="A8=1-52"/>
    </dbReference>
    <dbReference type="PDB" id="4V7I">
        <property type="method" value="EM"/>
        <property type="resolution" value="9.60 A"/>
        <property type="chains" value="AC=1-53"/>
    </dbReference>
    <dbReference type="PDBsum" id="1RH5"/>
    <dbReference type="PDBsum" id="1RHZ"/>
    <dbReference type="PDBsum" id="2YXQ"/>
    <dbReference type="PDBsum" id="2YXR"/>
    <dbReference type="PDBsum" id="3BO0"/>
    <dbReference type="PDBsum" id="3BO1"/>
    <dbReference type="PDBsum" id="3DKN"/>
    <dbReference type="PDBsum" id="4V4N"/>
    <dbReference type="PDBsum" id="4V7I"/>
    <dbReference type="AlphaFoldDB" id="P60460"/>
    <dbReference type="SMR" id="P60460"/>
    <dbReference type="IntAct" id="P60460">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="P60460"/>
    <dbReference type="TCDB" id="3.A.5.7.4">
        <property type="family name" value="the general secretory pathway (sec) family"/>
    </dbReference>
    <dbReference type="GeneID" id="27929979"/>
    <dbReference type="OMA" id="GLIRYMD"/>
    <dbReference type="OrthoDB" id="127915at2157"/>
    <dbReference type="EvolutionaryTrace" id="P60460"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="DisProt" id="DP00964"/>
    <dbReference type="HAMAP" id="MF_00751">
        <property type="entry name" value="SecG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023531">
        <property type="entry name" value="Preprot_translocase_SecG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000157268" description="Preprotein translocase subunit SecG">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="14661030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1RH5"/>
        </source>
    </evidence>
    <sequence length="53" mass="5959" checksum="D2D5DD22331BE2AF" modified="2004-02-16" version="1">MSKREETGLATSAGLIRYMDETFSKIRVKPEHVIGVTVAFVIIEAILTYGRFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>P81234</accession>
    <name>Y27B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0275.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0275.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0696.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869773.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81234"/>
    <dbReference type="SMR" id="P81234"/>
    <dbReference type="STRING" id="243232.MJ_0275.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98263">
        <property type="protein sequence ID" value="AAB98263"/>
        <property type="gene ID" value="MJ_0275.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451130"/>
    <dbReference type="KEGG" id="mja:MJ_0275.1"/>
    <dbReference type="eggNOG" id="arCOG09660">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754708_0_0_2"/>
    <dbReference type="OMA" id="REETFNW"/>
    <dbReference type="OrthoDB" id="122985at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106769" description="Uncharacterized protein MJ0275.1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="148" mass="17356" checksum="305E22EC236661CD" modified="1998-07-15" version="1">MCGIMRVYRVYNAYKIVGAVIFSMSIIVILYISIILHSLKLSFSIILAVDILIIALFAYIFLKPKKLVVLDNGIKVDNEFYSWDEVIEFFVSLNSIQINLKGKREETFNWETPGLFKYRPQIEYVVKKDAELLKILREKIENKERKRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57550</accession>
    <name>Y085_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized lipoprotein MJ0085</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0085</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64310">
        <property type="entry name" value="E64310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869577.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57550"/>
    <dbReference type="SMR" id="Q57550"/>
    <dbReference type="STRING" id="243232.MJ_0085"/>
    <dbReference type="EnsemblBacteria" id="AAB98067">
        <property type="protein sequence ID" value="AAB98067"/>
        <property type="gene ID" value="MJ_0085"/>
    </dbReference>
    <dbReference type="GeneID" id="1450924"/>
    <dbReference type="KEGG" id="mja:MJ_0085"/>
    <dbReference type="eggNOG" id="arCOG03303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038034_13_1_2"/>
    <dbReference type="InParanoid" id="Q57550"/>
    <dbReference type="OMA" id="WLMPEYA"/>
    <dbReference type="OrthoDB" id="90656at2157"/>
    <dbReference type="PhylomeDB" id="Q57550"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013991" description="Uncharacterized lipoprotein MJ0085">
        <location>
            <begin position="21"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="58"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-archaeol cysteine" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00344"/>
        </source>
    </evidence>
    <sequence length="373" mass="41812" checksum="8E64F5683E830878" modified="1997-11-01" version="1" precursor="true">MLKKLIGLLTILIIAVGFCGCMEQNIEKQTPTASEAPNTIKVVDLYGREVEVPKEVNRIVCCGPGCLRLIVYLNATDKVVGVEDTEKKWTPWTRPYRIAHPELANLPTIGQGGPCPKPNPEAIIQVKPDVIFVTYMPKDEVDALQQKTGIPVVVLSYGQLATFNNEDLFKSLELAGKILGKEERAKEVIEFIKNCQNDLNERTKDIPDDKKPSVYVGGIGYKGLHGIDSTECKYPPFVAVNAKNVADELGKEGHVFVTKEQILKWNPDIIFIDEGGLKLVVEDYKRNKEFYNSLKAFKNGDVYGLLPYNFYTTNIGTALADAYYIGKVVYPDRFKDIDPEQKADEIFTFLVGKPVYKEMKEKLGGFKKLEFSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q57565</accession>
    <name>SRP54_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="1">SRP54</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp54</name>
        <name type="ordered locus">MJ0101</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64312">
        <property type="entry name" value="E64312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869593.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2V3C">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="C/D=3-427"/>
    </dbReference>
    <dbReference type="PDB" id="3NDB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="B=3-431"/>
    </dbReference>
    <dbReference type="PDB" id="4XCO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="C/D=304-451"/>
    </dbReference>
    <dbReference type="PDBsum" id="2V3C"/>
    <dbReference type="PDBsum" id="3NDB"/>
    <dbReference type="PDBsum" id="4XCO"/>
    <dbReference type="AlphaFoldDB" id="Q57565"/>
    <dbReference type="SMR" id="Q57565"/>
    <dbReference type="DIP" id="DIP-46442N"/>
    <dbReference type="IntAct" id="Q57565">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0101"/>
    <dbReference type="EnsemblBacteria" id="AAB98081">
        <property type="protein sequence ID" value="AAB98081"/>
        <property type="gene ID" value="MJ_0101"/>
    </dbReference>
    <dbReference type="GeneID" id="1450940"/>
    <dbReference type="KEGG" id="mja:MJ_0101"/>
    <dbReference type="eggNOG" id="arCOG01228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_6_0_2"/>
    <dbReference type="InParanoid" id="Q57565"/>
    <dbReference type="OMA" id="DTAGRHK"/>
    <dbReference type="OrthoDB" id="25871at2157"/>
    <dbReference type="PhylomeDB" id="Q57565"/>
    <dbReference type="EvolutionaryTrace" id="Q57565"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="DisProt" id="DP00897"/>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="PTHR11564"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="SSF47446"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000101177" description="Signal recognition particle 54 kDa protein">
        <location>
            <begin position="1"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="188"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="247"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="4"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="18"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="28"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="45"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="53"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="60"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="70"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="131"/>
            <end position="134"/>
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        <location>
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    <feature type="helix" evidence="4">
        <location>
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    <feature type="helix" evidence="2">
        <location>
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    <feature type="helix" evidence="2">
        <location>
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        <location>
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    <feature type="helix" evidence="2">
        <location>
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    <feature type="helix" evidence="2">
        <location>
            <begin position="407"/>
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        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="424"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="434"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="437"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2V3C"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3NDB"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4XCO"/>
        </source>
    </evidence>
    <sequence length="451" mass="50124" checksum="5EEAF505FD227914" modified="1997-11-01" version="1">MLDKLGENLNKALNKLKAAAFVDKKLIKEVIKDIQRALIQADVNVKLVLKMSKEIERRALEEKTPKGLSKKEHIIKIVYEELVKLLGEEAKKLELNPKKQNVILLVGIQGSGKTTTAAKLARYIQKRGLKPALIAADTYRPAAYEQLKQLAEKIHVPIYGDETRTKSPVDIVKEGMEKFKKADVLIIDTAGRHKEEKGLLEEMKQIKEITNPDEIILVIDGTIGQQAGIQAKAFKEAVGEIGSIIVTKLDGSAKGGGALSAVAETKAPIKFIGIGEGIDDLEPFDPKKFISRLLGMGDLESLLEKAEDMVDEKTEESIDAIMRGKFTLNELMTQLEAIENMGSMKKILSMIPGFGGAMPKELSHLTEAKIKKYKVIISSMTKEERENPKIIKASRIRRIARGSGTTENDVREVLRYYETTKNAIDKLRKGKMLRIGGPLGQIMRQLMFKEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57590</accession>
    <name>Y126_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ0126</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ0126</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0126</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable antitoxin component of a putative type VII toxin-antitoxin (TA) system. Neutralizes cognate toxic MJ0125 by di-AMPylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Probably forms a complex with cognate toxin MJ0125.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869619.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57590"/>
    <dbReference type="SMR" id="Q57590"/>
    <dbReference type="STRING" id="243232.MJ_0126"/>
    <dbReference type="EnsemblBacteria" id="AAB98106">
        <property type="protein sequence ID" value="AAB98106"/>
        <property type="gene ID" value="MJ_0126"/>
    </dbReference>
    <dbReference type="GeneID" id="1450968"/>
    <dbReference type="KEGG" id="mja:MJ_0126"/>
    <dbReference type="eggNOG" id="arCOG01206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_10_3_2"/>
    <dbReference type="InParanoid" id="Q57590"/>
    <dbReference type="OMA" id="GEMNDDS"/>
    <dbReference type="OrthoDB" id="108900at2157"/>
    <dbReference type="PhylomeDB" id="Q57590"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106705" description="Putative protein adenylyltransferase MJ0126">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="98" mass="11605" checksum="0748F07131F29685" modified="1998-12-15" version="2">MKTLSEIKEILRKHKKELKEKYKVKSIAIFGSYARNEQTETSDIDILIDYYEPISLLKLIELENYLSDLLEIKVDLITKNSIHNPYVKKSIEEDLIYI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57601</accession>
    <name>Y137_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0137</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0137</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ1495.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64317">
        <property type="entry name" value="A64317"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57601"/>
    <dbReference type="SMR" id="Q57601"/>
    <dbReference type="STRING" id="243232.MJ_0137"/>
    <dbReference type="DNASU" id="1450978"/>
    <dbReference type="EnsemblBacteria" id="AAB98120">
        <property type="protein sequence ID" value="AAB98120"/>
        <property type="gene ID" value="MJ_0137"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0137"/>
    <dbReference type="eggNOG" id="arCOG01911">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067083_0_0_2"/>
    <dbReference type="InParanoid" id="Q57601"/>
    <dbReference type="OMA" id="DKIIMDP"/>
    <dbReference type="PhylomeDB" id="Q57601"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_sah"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="SSF52096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106713" description="Uncharacterized protein MJ0137">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="286" mass="32166" checksum="A43DD345B569C55F" modified="1997-11-01" version="1">MWGEKMDPLSGFISSLIWWLLFFYLIMAPQIQYKQLQLARLKILRELSNKRNSTVITMIHRQESIGLFGIPVYKFITIEDSEEILRAIRAAPKDKPIDLIIHTPGGLVLAATQIAKALKAHPAETRVIVPHYAMSGGTLIALAADKIIMDENAVLGPVDPQLGQYPAPSIVKAVEQKGADKADDQTLILADIAKKAINQVQNFVYNLLKDKYGEEKAKELSKILTEGRWTHDYPITVEEAKELGLDVDTNVPEEVYTLMELYKQPVRQRGTVEFMPYPVKQENGAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q57612</accession>
    <name>TRUB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase B</fullName>
            <ecNumber evidence="1">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(55) synthase</fullName>
            <shortName evidence="1">Psi55 synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truB</name>
        <name type="ordered locus">MJ0148</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9026474">
            <id>Q57612</id>
        </interactant>
        <interactant intactId="EBI-9026465">
            <id>P81303</id>
            <label>nop10</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>5</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruB family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64318">
        <property type="entry name" value="E64318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869643.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2APO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=1-336"/>
    </dbReference>
    <dbReference type="PDBsum" id="2APO"/>
    <dbReference type="AlphaFoldDB" id="Q57612"/>
    <dbReference type="SMR" id="Q57612"/>
    <dbReference type="DIP" id="DIP-60499N"/>
    <dbReference type="IntAct" id="Q57612">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0148"/>
    <dbReference type="EnsemblBacteria" id="AAB98132">
        <property type="protein sequence ID" value="AAB98132"/>
        <property type="gene ID" value="MJ_0148"/>
    </dbReference>
    <dbReference type="GeneID" id="1450992"/>
    <dbReference type="KEGG" id="mja:MJ_0148"/>
    <dbReference type="eggNOG" id="arCOG00987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032087_3_0_2"/>
    <dbReference type="InParanoid" id="Q57612"/>
    <dbReference type="OMA" id="GPFKEDE"/>
    <dbReference type="OrthoDB" id="36028at2157"/>
    <dbReference type="PhylomeDB" id="Q57612"/>
    <dbReference type="EvolutionaryTrace" id="Q57612"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01081">
        <property type="entry name" value="TruB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026326">
        <property type="entry name" value="TruB_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="PTHR23127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000121959" description="Probable tRNA pseudouridine synthase B">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="248"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="18"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="39"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="46"/>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="74"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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            <end position="92"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="107"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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            <end position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
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        </location>
    </feature>
    <feature type="strand" evidence="2">
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="179"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="209"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="232"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="238"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="241"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="248"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="255"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="258"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="269"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="272"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="284"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="294"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="304"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01081"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2APO"/>
        </source>
    </evidence>
    <sequence length="336" mass="38483" checksum="F29E3EC979A5A89A" modified="1996-11-01" version="1">MILLEKTQEKKINDKEELIVKEEVETNWDYGCNPYERKIEDLIKYGVVVVDKPRGPTSHEVSTWVKKILNLDKAGHGGTLDPKVTGVLPVALERATKTIPMWHIPPKEYVCLMHLHRDASEEDILRVFKEFTGRIYQRPPLKAAVKRRLRIRKIHELELLDKDGKDVLFRVKCQSGTYIRKLCEDIGEALGTSAHMQELRRTKSGCFEEKDAVYLQDLLDAYVFWKEDGDEEELRRVIKPMEYGLRHLKKVVVKDSAVDAICHGADVYVRGIAKLSKGIGKGETVLVETLKGEAVAVGKALMNTKEILNADKGVAVDVERVYMDRGTYPRMWKRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q57613</accession>
    <name>Y149_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0149</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0149</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64318">
        <property type="entry name" value="F64318"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57613"/>
    <dbReference type="STRING" id="243232.MJ_0149"/>
    <dbReference type="EnsemblBacteria" id="AAB98140">
        <property type="protein sequence ID" value="AAB98140"/>
        <property type="gene ID" value="MJ_0149"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0149"/>
    <dbReference type="eggNOG" id="arCOG08276">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_204983_0_0_2"/>
    <dbReference type="OMA" id="IFCAFGR"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032820">
        <property type="entry name" value="ATPase_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09527">
        <property type="entry name" value="ATPase_gene1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106719" description="Uncharacterized protein MJ0149">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="58" mass="6917" checksum="CD7CB74F2F2CCC3E" modified="1996-11-01" version="1">MLRDIAFEFFIMIALGIFIGYIIAEYTDNNLWIVVFLLLGIFCAFGRLFKMIKDYEKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57614</accession>
    <name>Y150_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0150</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0150</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the M.jannaschii MJ0150/MJ0739/MJ0745/MJ1460/MJ1642 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64318">
        <property type="entry name" value="G64318"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57614"/>
    <dbReference type="SMR" id="Q57614"/>
    <dbReference type="STRING" id="243232.MJ_0150"/>
    <dbReference type="EnsemblBacteria" id="AAB98141">
        <property type="protein sequence ID" value="AAB98141"/>
        <property type="gene ID" value="MJ_0150"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0150"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128702_0_0_2"/>
    <dbReference type="InParanoid" id="Q57614"/>
    <dbReference type="OMA" id="GTTLCHF"/>
    <dbReference type="PhylomeDB" id="Q57614"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106720" description="Uncharacterized protein MJ0150">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="173" mass="19524" checksum="A273F382C91D0315" modified="1996-11-01" version="1">MGDVKMDKELLHKKIKKDIEDLINNHPPERTLGNLIPLSIFQAVRIGVLTAGCGIEAIIYNIGKDIGREVISRYVDRDNLLESFAEILKKAKIGILEVKKVEENEMILILKDCISCHNVPNVGTTLCHFEAGLIAGTLEKKLRRKVNAVETKCCGKGDEYCEFLVKIEDKLYW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57625</accession>
    <name>ILVH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable acetolactate synthase small subunit</fullName>
            <ecNumber>2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Acetohydroxy-acid synthase small subunit</fullName>
            <shortName>AHAS</shortName>
            <shortName>ALS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ilvH</name>
        <name type="synonym">ilvN</name>
        <name type="ordered locus">MJ0161</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64320">
        <property type="entry name" value="B64320"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57625"/>
    <dbReference type="SMR" id="Q57625"/>
    <dbReference type="STRING" id="243232.MJ_0161"/>
    <dbReference type="EnsemblBacteria" id="AAB98145">
        <property type="protein sequence ID" value="AAB98145"/>
        <property type="gene ID" value="MJ_0161"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0161"/>
    <dbReference type="eggNOG" id="arCOG04445">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055003_1_3_2"/>
    <dbReference type="InParanoid" id="Q57625"/>
    <dbReference type="OMA" id="EPYGIKE"/>
    <dbReference type="PhylomeDB" id="Q57625"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="PTHR30239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000151423" description="Probable acetolactate synthase small subunit">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="2">
        <location>
            <begin position="10"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="172" mass="19057" checksum="8C00191BD2B18303" modified="1996-11-01" version="1">MVNIMEHRHVISALVLNKPGVLQRISGLFTRRGFNISSITVGITENPQISRVTIVVNGDDKILEQVIKQLNKLIDVIKVSELEEKKSVQRELCLIKIYAPTESAKSQVIQYTSIFRGNVVDLSPESLIVEITGSEDKINAFIDLVKPLGIKEMARTGITALARGPKILKPKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q57634</accession>
    <name>MSMJS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Small-conductance mechanosensitive channel MscMJ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0170</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Biophys. J." volume="80" first="229" last="240">
            <title>Molecular identification of a mechanosensitive channel in archaea.</title>
            <authorList>
                <person name="Kloda A."/>
                <person name="Martinac B."/>
            </authorList>
            <dbReference type="PubMed" id="11159397"/>
            <dbReference type="DOI" id="10.1016/S0006-3495(01)76009-2"/>
        </citation>
        <scope>FUNCTION AS A MECHANOSENSITIVE CHANNEL</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Small-conductance mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Exhibits a sixfold preference for cations over anions. Non-rectifying.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64321">
        <property type="entry name" value="C64321"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57634"/>
    <dbReference type="SMR" id="Q57634"/>
    <dbReference type="STRING" id="243232.MJ_0170"/>
    <dbReference type="TCDB" id="1.A.23.4.2">
        <property type="family name" value="the small conductance mechanosensitive ion channel (mscs) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98155">
        <property type="protein sequence ID" value="AAB98155"/>
        <property type="gene ID" value="MJ_0170"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0170"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_0_1_2"/>
    <dbReference type="InParanoid" id="Q57634"/>
    <dbReference type="OMA" id="NIEFAFP"/>
    <dbReference type="PhylomeDB" id="Q57634"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006686">
        <property type="entry name" value="MscS_channel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01246">
        <property type="entry name" value="UPF0003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000110253" description="Small-conductance mechanosensitive channel MscMJ">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11159397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="350" mass="39953" checksum="4EA0E5025086E7DF" modified="1996-11-01" version="1">MNMEIFGNSISNILIFVVITLLGIFIGKIVDKIVRNYLKKIIDKTKTKFDDIILESIDLPIIVLVVTLFFYFGLRFLILPDYILKLIDEAVKVVVILSATYFAVKFIDGIFEHYLIPLTEKTETELDEHIIKPLKKVVKILTILLGILTALSSVGYDITALLAGLGVGGLALALAMQDTIKNFIAGILILIDKPFSLGHWVKVKGAEGIVEEIGIRSTRIRTFDYTLITIPNSELLDSAIENLTVRDRRRVLMTIGLTYNTPVEKIKRAKEIIKEIVENHPATLPPYRVHFREYGDWSLNLRVEYFVRNMGFDYYLNAVDEINLKIKEEFEKEGIEMAFPTYTVYLEKDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q57660</accession>
    <name>Y207_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0207</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0207</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64325">
        <property type="entry name" value="H64325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869702.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57660"/>
    <dbReference type="STRING" id="243232.MJ_0207"/>
    <dbReference type="EnsemblBacteria" id="AAB98194">
        <property type="protein sequence ID" value="AAB98194"/>
        <property type="gene ID" value="MJ_0207"/>
    </dbReference>
    <dbReference type="GeneID" id="1451056"/>
    <dbReference type="KEGG" id="mja:MJ_0207"/>
    <dbReference type="eggNOG" id="arCOG07626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727252_0_0_2"/>
    <dbReference type="OMA" id="RICGYEF"/>
    <dbReference type="OrthoDB" id="97250at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106741" description="Uncharacterized protein MJ0207">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <sequence length="151" mass="16912" checksum="03F78A3F2EBCA189" modified="1996-11-01" version="1">MVLYKIRRSKNDPCPSIPSAVIIGYSVGLKLITGHGAQSLSNMAGSYAGKELGIYAMNNGYEFKDIKDIERFLNQLDFAKIEMNEEEDEIIVKISKCNLCPKRICGYEFEGTACPWGGLLIGFISETLKYNLGYQMNLKPAETCIIKLKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57661</accession>
    <name>DMRX_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydromethanopterin reductase (acceptor)</fullName>
            <shortName evidence="4">H(2)MPT reductase</shortName>
            <ecNumber evidence="3">1.5.99.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dmrX</name>
        <name type="ordered locus">MJ0208</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="J. Bacteriol." volume="196" first="203" last="209">
            <title>Discovery and characterization of the first archaeal dihydromethanopterin reductase, an iron-sulfur flavoprotein from Methanosarcina mazei.</title>
            <authorList>
                <person name="Wang S."/>
                <person name="Tiongson J."/>
                <person name="Rasche M.E."/>
            </authorList>
            <dbReference type="PubMed" id="23995635"/>
            <dbReference type="DOI" id="10.1128/JB.00457-13"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>COFACTOR</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the biosynthesis of tetrahydromethanopterin, a coenzyme used in methanogenesis. Catalyzes the reduction of dihydromethanopterin (H(2)MPT) to tetrahydromethanopterin (H(4)MPT). Ferredoxin may serve as an electron donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5,6,7,8-tetrahydromethanopterin + A = 7,8-dihydromethanopterin + AH2</text>
            <dbReference type="Rhea" id="RHEA:42804"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:72788"/>
            <dbReference type="EC" id="1.5.99.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="1.5.99.15" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64326">
        <property type="entry name" value="A64326"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57661"/>
    <dbReference type="SMR" id="Q57661"/>
    <dbReference type="STRING" id="243232.MJ_0208"/>
    <dbReference type="EnsemblBacteria" id="AAB98191">
        <property type="protein sequence ID" value="AAB98191"/>
        <property type="gene ID" value="MJ_0208"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0208"/>
    <dbReference type="eggNOG" id="arCOG01705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098523_0_0_2"/>
    <dbReference type="InParanoid" id="Q57661"/>
    <dbReference type="OMA" id="IVWCITG"/>
    <dbReference type="PhylomeDB" id="Q57661"/>
    <dbReference type="BRENDA" id="1.5.99.15">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044684">
        <property type="term" value="F:dihydromethanopterin reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901285">
        <property type="term" value="P:5,6,7,8-tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014073">
        <property type="entry name" value="DmrX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02700">
        <property type="entry name" value="flavo_MJ0208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159308" description="Dihydromethanopterin reductase (acceptor)">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="179"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="23995635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="23995635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="246" mass="27697" checksum="41DAC513C3212721" modified="1996-11-01" version="1">MNYRFGINMKIVWCITGAGHLLRESFQVMKRLKEEIEDLKVTTLVSRAGEEVVKMYGLFGELYNISNGNYYEELILEREHPYSSPITGRLSLGKYDYLICSPATGNTVAKVVNGIADSLVTNAIAQAGKGFVKSLIVPVDYKAGIVTTKLPYAIDKKKCKLCLKCINVCPNGAIVKRDNFVEILLSKCLGCGNCKKVCPYNAIIEGKEIKMRVRKIDAENTRKLMELEDVIVLKHPYEILEFFNIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q57668</accession>
    <name>Y215_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0215</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0215</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJECL20.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64326">
        <property type="entry name" value="H64326"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57668"/>
    <dbReference type="SMR" id="Q57668"/>
    <dbReference type="STRING" id="243232.MJ_0215"/>
    <dbReference type="EnsemblBacteria" id="AAB98205">
        <property type="protein sequence ID" value="AAB98205"/>
        <property type="gene ID" value="MJ_0215"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0215"/>
    <dbReference type="eggNOG" id="arCOG03413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1700308_0_0_2"/>
    <dbReference type="InParanoid" id="Q57668"/>
    <dbReference type="OMA" id="RERYCEC"/>
    <dbReference type="PhylomeDB" id="Q57668"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="SSF52799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106745" description="Uncharacterized protein MJ0215">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="159" mass="18397" checksum="5FAD7EB90DE3A8D3" modified="1997-11-01" version="1">MYIAILKCESMGRCKHNGEVSIFGVRPASFPNFPFHLMDKIGGFVILDELWLRRWCEIIEYPMRIPTLYVPIEDYGIPTVEDMDLIVDFIKYHVSKEKEVVVSCIGGHGRTGTVLAVWAGLNGIKNPIEYVRERYCECAVETEEQEEFVIEYLKMKKRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q57680</accession>
    <name>Y227_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0227</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0227</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64328">
        <property type="entry name" value="D64328"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57680"/>
    <dbReference type="SMR" id="Q57680"/>
    <dbReference type="STRING" id="243232.MJ_0227"/>
    <dbReference type="DNASU" id="1451080"/>
    <dbReference type="EnsemblBacteria" id="AAB98212">
        <property type="protein sequence ID" value="AAB98212"/>
        <property type="gene ID" value="MJ_0227"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0227"/>
    <dbReference type="eggNOG" id="arCOG04238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063627_0_0_2"/>
    <dbReference type="InParanoid" id="Q57680"/>
    <dbReference type="OMA" id="GNTHFGA"/>
    <dbReference type="PhylomeDB" id="Q57680"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106750" description="Uncharacterized protein MJ0227">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <sequence length="308" mass="35190" checksum="E191FB42772BCE58" modified="1996-11-01" version="1">MHLPNSQIYKNFGESMNPFDIMKASQIACCLEVSSFKPGNVHRNRDYRDIKYHHFINAGIAFGNVVYEAAQKDRDVGLYIKKAVIESKKWSPTNANLGIIMLHIPIAMAAGKLENFDENKLKENLKKIAENTTVEDALNVYDAINIAMAYVNKPKKGPDVTSEDAKKELIEKGLTLLDVYKISAEWDNISKEWVDNFKISFEGYNLLKKYYDELNNINLAVTKTFLNLLAKYPDTLIARKRGFETALKVSKMAEDVLNNFKEEKVKEFDKYLSKEGNKLNPGTTADLIASSLMIFILDRIDKRETILW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57729</accession>
    <name>AMZA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Archaemetzincin</fullName>
            <ecNumber evidence="1">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">amzA</name>
        <name type="ordered locus">MJ0281</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable zinc metalloprotease whose natural substrate is unknown.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit. One is catalytic, whereas the other seems to have a structural role.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64335">
        <property type="entry name" value="B64335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57729"/>
    <dbReference type="SMR" id="Q57729"/>
    <dbReference type="STRING" id="243232.MJ_0281"/>
    <dbReference type="MEROPS" id="M54.001"/>
    <dbReference type="EnsemblBacteria" id="AAB98269">
        <property type="protein sequence ID" value="AAB98269"/>
        <property type="gene ID" value="MJ_0281"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0281"/>
    <dbReference type="eggNOG" id="arCOG00458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108521_2_0_2"/>
    <dbReference type="InParanoid" id="Q57729"/>
    <dbReference type="OMA" id="KDRGQYH"/>
    <dbReference type="PhylomeDB" id="Q57729"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11375">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01842">
        <property type="entry name" value="Archaemetzincin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012091">
        <property type="entry name" value="Pept_M54_archaemetzncn_arc/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15910">
        <property type="entry name" value="PTHR15910"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07998">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005785">
        <property type="entry name" value="Zn-prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159626" description="Archaemetzincin">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01842"/>
        </source>
    </evidence>
    <sequence length="179" mass="20514" checksum="895A7E935A757CE8" modified="1996-11-01" version="1">MVMIMRICIQPVGDVNDEILKFLKKKFGEVFGMCEILPKIDIPIYAYNFSRGQFNSTLILKSLPTVEDIVLGVTEVDIYADNLNFVFGEAELFGKRALISLARLRPEFYGLPPNKDVLKIRALKEAIHEIGHVLGLIHCENKRCVMSFSNSIIDVDLKDWRYCKKCLKKLQDRGIYISI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57737</accession>
    <name>Y289_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0289</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0289</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64336">
        <property type="entry name" value="B64336"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57737"/>
    <dbReference type="STRING" id="243232.MJ_0289"/>
    <dbReference type="EnsemblBacteria" id="AAB98282">
        <property type="protein sequence ID" value="AAB98282"/>
        <property type="gene ID" value="MJ_0289"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0289"/>
    <dbReference type="eggNOG" id="arCOG09663">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_840947_0_0_2"/>
    <dbReference type="OMA" id="FYAQWTV"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106776" description="Uncharacterized protein MJ0289">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="338" mass="36045" checksum="74A5CE21F5273AEC" modified="1996-11-01" version="1">MFEMKNSTRYILSLLLSIIMGVAVMGSTFAISTTYGTGHTTATVDNLKPVVNCSSYEMVIRTVQGIKVYEYKNTTGVTPGLLRSDALEAYAYTGEGVTFYVNVSDPNGEQDLQTNGAGVDFLLVPQGQSPSNPTYVIHAGFDTSTSGDADLTTLTFYAQWTVPAGAYGCFDVYVKATDKHGACTGYIKKGKIFLNPMIGINVTKDNDAYPAPFTGLSFGNVNPGDTNVPATENVVTIHNIDPDGVGTKIAVFVSATSMTQAGGTGIIPAENIKAHVIKANNMTQSYNTHLQNNVKVLLWQPLKPCHTNALEVNFTLDVPTPLPSGCYGGSITFYGLGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q57764</accession>
    <name>PDAD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Pyruvoyl-dependent arginine decarboxylase</fullName>
            <shortName>PvlArgDC</shortName>
            <ecNumber>4.1.1.19</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Pyruvoyl-dependent arginine decarboxylase subunit beta</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Pyruvoyl-dependent arginine decarboxylase subunit alpha</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">pdaD</name>
        <name type="ordered locus">MJ0316</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="23500" last="23507">
            <title>Methanococcus jannaschii uses a pyruvoyl-dependent arginine decarboxylase in polyamine biosynthesis.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="11980912"/>
            <dbReference type="DOI" id="10.1074/jbc.M203467200"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>PYRUVATE FORMATION AT SER-53</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>H(+) + L-arginine = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text>Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text>Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text>Trimer of an alpha-beta dimer.</text>
    </comment>
    <comment type="mass spectrometry" mass="5436.0" error="11.0" method="MALDI" evidence="1">
        <molecule>Pyruvoyl-dependent arginine decarboxylase subunit beta</molecule>
    </comment>
    <comment type="mass spectrometry" mass="12356.0" error="57.0" method="MALDI" evidence="1">
        <molecule>Pyruvoyl-dependent arginine decarboxylase subunit alpha</molecule>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PdaD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64339">
        <property type="entry name" value="E64339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869814.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1MT1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/C/E/G/I/K=1-52, B/D/F/H/J/L=53-165"/>
    </dbReference>
    <dbReference type="PDB" id="1N13">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="A/C/E/G/I/K=1-52, B/D/F/H/J/L=53-165"/>
    </dbReference>
    <dbReference type="PDB" id="1N2M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="2QQC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/C/E/G/I/K=1-52, B/D/F/H/J/L=54-165"/>
    </dbReference>
    <dbReference type="PDB" id="2QQD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/D=1-52, B/E=54-165, C/F/G/H=1-165"/>
    </dbReference>
    <dbReference type="PDBsum" id="1MT1"/>
    <dbReference type="PDBsum" id="1N13"/>
    <dbReference type="PDBsum" id="1N2M"/>
    <dbReference type="PDBsum" id="2QQC"/>
    <dbReference type="PDBsum" id="2QQD"/>
    <dbReference type="AlphaFoldDB" id="Q57764"/>
    <dbReference type="SMR" id="Q57764"/>
    <dbReference type="STRING" id="243232.MJ_0316"/>
    <dbReference type="EnsemblBacteria" id="AAB98302">
        <property type="protein sequence ID" value="AAB98302"/>
        <property type="gene ID" value="MJ_0316"/>
    </dbReference>
    <dbReference type="GeneID" id="1451171"/>
    <dbReference type="KEGG" id="mja:MJ_0316"/>
    <dbReference type="eggNOG" id="arCOG04490">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114389_2_0_2"/>
    <dbReference type="InParanoid" id="Q57764"/>
    <dbReference type="OMA" id="AAALWYK"/>
    <dbReference type="OrthoDB" id="98606at2157"/>
    <dbReference type="PhylomeDB" id="Q57764"/>
    <dbReference type="BRENDA" id="4.1.1.19">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q57764"/>
    <dbReference type="EvolutionaryTrace" id="Q57764"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01404">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PTHR40438"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="SSF56271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00286">
        <property type="entry name" value="TIGR00286"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000023314" description="Pyruvoyl-dependent arginine decarboxylase subunit beta">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000023315" description="Pyruvoyl-dependent arginine decarboxylase subunit alpha">
        <location>
            <begin position="53"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic)">
        <location>
            <begin position="52"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="46"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="105"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="138"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="151"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11980912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1N13"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2QQD"/>
        </source>
    </evidence>
    <sequence length="165" mass="17708" checksum="04C7F8AB3AEC2342" modified="1996-11-01" version="1">MNAEINPLHAYFKLPNTVSLVAGSSEGETPLNAFDGALLNAGIGNVNLIRISSIMPPEAEIVPLPKLPMGALVPTAYGYIISDVPGETISAAISVAIPKDKSLCGLIMEYEGKCSKKEAEKTVREMAKIGFEMRGWELDRIESIAVEHTVEKLGCAFAAAALWYK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q57775</accession>
    <name>Y329_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0329</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0329</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64341">
        <property type="entry name" value="A64341"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869826.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57775"/>
    <dbReference type="STRING" id="243232.MJ_0329"/>
    <dbReference type="EnsemblBacteria" id="AAB98317">
        <property type="protein sequence ID" value="AAB98317"/>
        <property type="gene ID" value="MJ_0329"/>
    </dbReference>
    <dbReference type="GeneID" id="1451184"/>
    <dbReference type="KEGG" id="mja:MJ_0329"/>
    <dbReference type="eggNOG" id="arCOG09510">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_446653_0_0_2"/>
    <dbReference type="OMA" id="ERIFPSN"/>
    <dbReference type="OrthoDB" id="132625at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006528">
        <property type="entry name" value="Phage_head_morphogenesis_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04233">
        <property type="entry name" value="Phage_Mu_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01641">
        <property type="entry name" value="phageSPP1_gp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106798" description="Uncharacterized protein MJ0329">
        <location>
            <begin position="1"/>
            <end position="616"/>
        </location>
    </feature>
    <sequence length="616" mass="72037" checksum="2991EC36D4D64B82" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57822</accession>
    <name>CAS4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated exonuclease Cas4</fullName>
            <ecNumber evidence="1">3.1.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas4</name>
        <name type="ordered locus">MJ0377</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein may be a 5' to 3' ssDNA exonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>exonucleolytic cleavage in the 5'- to 3'-direction to yield nucleoside 3'-phosphates.</text>
            <dbReference type="EC" id="3.1.12.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
        <text evidence="1">Mg(2+) or Mn(2+) required for ssDNA cleavage activity. Can also utilise Cu(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. It may be important for protein stability, since mutation of the Cys that bind the cofactor leads to a colorless, insoluble protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated exonuclease Cas4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.12.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64347">
        <property type="entry name" value="A64347"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869876.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57822"/>
    <dbReference type="SMR" id="Q57822"/>
    <dbReference type="STRING" id="243232.MJ_0377"/>
    <dbReference type="EnsemblBacteria" id="AAB98366">
        <property type="protein sequence ID" value="AAB98366"/>
        <property type="gene ID" value="MJ_0377"/>
    </dbReference>
    <dbReference type="GeneID" id="1451234"/>
    <dbReference type="KEGG" id="mja:MJ_0377"/>
    <dbReference type="eggNOG" id="arCOG00794">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133784_0_0_2"/>
    <dbReference type="InParanoid" id="Q57822"/>
    <dbReference type="OMA" id="ICKTKLW"/>
    <dbReference type="OrthoDB" id="115986at2157"/>
    <dbReference type="PhylomeDB" id="Q57822"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045145">
        <property type="term" value="F:single-stranded DNA 5'-3' exodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000738">
        <property type="term" value="P:DNA catabolic process, exonucleolytic"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013343">
        <property type="entry name" value="CRISPR-assoc_prot_Cas4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022765">
        <property type="entry name" value="Dna2/Cas4_DUF83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011604">
        <property type="entry name" value="PDDEXK-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01930">
        <property type="entry name" value="Cas_Cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00372">
        <property type="entry name" value="cas4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106840" description="CRISPR-associated exonuclease Cas4">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q97TX9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="170" mass="20420" checksum="10CEBC592550AA4B" modified="1996-11-01" version="1">MENYLEEELIIGGIEINYLYVCKTKLWYFVRGITMEQESDFVDLGKFLHEKSYFGEEKEVQIGSIKIDFIKKRDVIEIHEVKRGKQMEKAHIMQVLYYIYYLNSLGIKSKAILHYPKLKEIKEIELKENNKEEIKRAIKEIEYIKSLKEPPEPIYQKICKNCAYYELCFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-02-23" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q57833</accession>
    <name>Y388_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0388</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0388</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64348">
        <property type="entry name" value="D64348"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869887.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0388"/>
    <dbReference type="EnsemblBacteria" id="AAB98374">
        <property type="protein sequence ID" value="AAB98374"/>
        <property type="gene ID" value="MJ_0388"/>
    </dbReference>
    <dbReference type="GeneID" id="1451245"/>
    <dbReference type="KEGG" id="mja:MJ_0388"/>
    <dbReference type="eggNOG" id="arCOG00908">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1335063_0_0_2"/>
    <dbReference type="InParanoid" id="Q57833"/>
    <dbReference type="OMA" id="HNKYNKD"/>
    <dbReference type="OrthoDB" id="79857at2157"/>
    <dbReference type="PhylomeDB" id="Q57833"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019202">
        <property type="entry name" value="DUF2067"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09840">
        <property type="entry name" value="DUF2067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106850" description="Uncharacterized protein MJ0388">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <sequence length="207" mass="24118" checksum="F33B51B95271F6D7" modified="1996-11-01" version="1">MRKIISSKVSCDEELLELCERLSRMDIDCTIESKGNRVRVYVFGYDKDSLKXNYRTIREVMEKVKRKYQKDDEGLYKYPLFELKYPVNKNLIIDALKTLGYKVIYLEDENAIKTNVDINKFNEILGELHELSQELRFSNLGSKPVKNLVVLVSYITKKPVDDVIEEALEKGFFREEEGRIVLNKDINLAKKALLEGEDGDKDIGEER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57841</accession>
    <name>Y398_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0398</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0398</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64349">
        <property type="entry name" value="F64349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869897.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57841"/>
    <dbReference type="SMR" id="Q57841"/>
    <dbReference type="EnsemblBacteria" id="AAB98393">
        <property type="protein sequence ID" value="AAB98393"/>
        <property type="gene ID" value="MJ_0398"/>
    </dbReference>
    <dbReference type="GeneID" id="1451257"/>
    <dbReference type="KEGG" id="mja:MJ_0398"/>
    <dbReference type="eggNOG" id="arCOG03156">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_914059_0_0_2"/>
    <dbReference type="InParanoid" id="Q57841"/>
    <dbReference type="OMA" id="YIIGVYF"/>
    <dbReference type="OrthoDB" id="61639at2157"/>
    <dbReference type="PhylomeDB" id="Q57841"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106852" description="Uncharacterized protein MJ0398">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="1">
        <location>
            <begin position="77"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <sequence length="271" mass="32120" checksum="63C4432642CB3644" modified="1996-11-01" version="1">MVNLKELSQNEVLELINYVKSLRKQNFSYSQISKKIEIERNIKISKSTIIRWCKNSNNPFNKTKFIDLSPSPELSYIIGVYFGDANIYYRKKTGSYYFRIKVVDKDFVDVVKNSLIKIGLNPTISYVEEKTRSNRWHVEASSKSLYKFLSQNKEELFKVAEKYPEDFLRGFFDSEGYVTSNKIALENYDLELLEFSKELLKKLDVHSTIHIAKKKGTESNIRGEIYHYKDDFYRLSIHRKESVRNFAIKVSFSIKRKRERLQKLLESMDKH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57883</accession>
    <name>Y441_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable membrane transporter protein MJ0441</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0441</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64355">
        <property type="entry name" value="A64355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869940.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57883"/>
    <dbReference type="STRING" id="243232.MJ_0441"/>
    <dbReference type="EnsemblBacteria" id="AAB98428">
        <property type="protein sequence ID" value="AAB98428"/>
        <property type="gene ID" value="MJ_0441"/>
    </dbReference>
    <dbReference type="GeneID" id="1451301"/>
    <dbReference type="KEGG" id="mja:MJ_0441"/>
    <dbReference type="eggNOG" id="arCOG02050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045498_6_0_2"/>
    <dbReference type="InParanoid" id="Q57883"/>
    <dbReference type="OMA" id="MYHHIIN"/>
    <dbReference type="OrthoDB" id="93738at2157"/>
    <dbReference type="PhylomeDB" id="Q57883"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000106878" description="Probable membrane transporter protein MJ0441">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="267" mass="28980" checksum="08EF7F36D198FCF0" modified="1996-11-01" version="1">MVIKLEFEFLLLLPLLIIVGFIVGILGSLFGIGGGFLVAPILTFIFDYFGIPDGVKFAVGTSLFVVFINSIISIFRHAKIKNINWKASITIGIISLVFSYFSGFLVVNFIDSAILKKLFGIFLIANAIYMAKSHHIDKISDREDKLEPFILCGVITGFLSGLFGIGGGIVIIPILAMAKYPVKRAVAISVGVIPLTSIGGLISYLTANTEGYIYNIGYVSIPIALIMAIPIIYSSKLGIKINQKISPKHLRIMLSSILGVMGLFMLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q57892</accession>
    <name>Y450_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0450</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64356">
        <property type="entry name" value="B64356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869949.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57892"/>
    <dbReference type="SMR" id="Q57892"/>
    <dbReference type="STRING" id="243232.MJ_0450"/>
    <dbReference type="EnsemblBacteria" id="AAB98439">
        <property type="protein sequence ID" value="AAB98439"/>
        <property type="gene ID" value="MJ_0450"/>
    </dbReference>
    <dbReference type="GeneID" id="1451311"/>
    <dbReference type="KEGG" id="mja:MJ_0450"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124806_0_0_2"/>
    <dbReference type="InParanoid" id="Q57892"/>
    <dbReference type="OMA" id="FEPGMSA"/>
    <dbReference type="OrthoDB" id="97248at2157"/>
    <dbReference type="PhylomeDB" id="Q57892"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000106881" description="Uncharacterized protein MJ0450">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="10"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="77"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <sequence length="186" mass="20791" checksum="472E1C56DDB607AB" modified="1996-11-01" version="1">MVGEIPVLLIMKKPIVVSGDVSVYDVAKLMVEQDVPCVLVVCERPNHESIEVATDKDIIKKVLIRKLPPDKVKVEDISSGKLVTIPPNTTIDEALEIMNKYKTNELFIVDDGKIVGVITEEDLIKIAPEIISTLKELVNYLLQIIDEVTSGDISDKSKEIQNINQGKDNKKDSESDIRKKKIMLIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q57911</accession>
    <name>SYFA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheS</name>
        <name type="ordered locus">MJ0487</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64360">
        <property type="entry name" value="G64360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869988.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57911"/>
    <dbReference type="SMR" id="Q57911"/>
    <dbReference type="STRING" id="243232.MJ_0487"/>
    <dbReference type="PRIDE" id="Q57911"/>
    <dbReference type="EnsemblBacteria" id="AAB98478">
        <property type="protein sequence ID" value="AAB98478"/>
        <property type="gene ID" value="MJ_0487"/>
    </dbReference>
    <dbReference type="GeneID" id="1451349"/>
    <dbReference type="KEGG" id="mja:MJ_0487"/>
    <dbReference type="eggNOG" id="arCOG00410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025086_2_2_2"/>
    <dbReference type="InParanoid" id="Q57911"/>
    <dbReference type="OMA" id="QIEGWVM"/>
    <dbReference type="OrthoDB" id="22747at2157"/>
    <dbReference type="PhylomeDB" id="Q57911"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000126810" description="Phenylalanine--tRNA ligase alpha subunit">
        <location>
            <begin position="1"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with beta subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <sequence length="480" mass="57027" checksum="1DC2599CF0D97B72" modified="1996-11-01" version="1">MELHIDEKRLLKIFQDNNRDEFNLNELEKFMPKEKILRVSLWLKGKNLVETEEKVKKIIKLIKEEEFPERKIANYLKQHNIKEIEIKNLKDILPKEEINAALGAIKRKGIARIEKGKIIFDNLDYKDVEEQLLQKIKENKYLDDFSEEEKKIIDILKKRGYVDFDEEKEIKIKLTEKGKEFIKNPIEIEEEITQLTRDIIISGKWKKAYIRPYDVKVPTKPIYPAKVHPLTRIIREVKEILLAMGFKEVKSPIVETEFWNFDMLFEPQDHPAREMQDTFFLKYPNEGDIPEDLLSKVKEVHERCWKYKFDENVSRRLILRTHTTASSIRYLASLSDEEKNKPHKVFCIDRVFRNEAIDYKHLPEFYQCEGIIMDDNVNFNNLIGVLKEFLNRLGFEKVRFRPAYFPFTEPSLEAEVYLEGKGWLEILGAGIFRPEVLEPIGIEKPVLAWGIGFSRLAMLRYGLTDIRDLHKNDLDWLKRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q57940</accession>
    <name>Y520_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative NADH-ubiquinone oxidoreductase MJ0520</fullName>
            <ecNumber>7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0520</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I subunit 1 family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64364">
        <property type="entry name" value="H64364"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57940"/>
    <dbReference type="SMR" id="Q57940"/>
    <dbReference type="STRING" id="243232.MJ_0520"/>
    <dbReference type="EnsemblBacteria" id="AAB98508">
        <property type="protein sequence ID" value="AAB98508"/>
        <property type="gene ID" value="MJ_0520"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0520"/>
    <dbReference type="eggNOG" id="arCOG01545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_0_2_2"/>
    <dbReference type="InParanoid" id="Q57940"/>
    <dbReference type="OMA" id="KIMARIQ"/>
    <dbReference type="PhylomeDB" id="Q57940"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0830">Ubiquinone</keyword>
    <feature type="chain" id="PRO_0000117536" description="Putative NADH-ubiquinone oxidoreductase MJ0520">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="295" mass="31909" checksum="F9903DBC914DFAD6" modified="1996-11-01" version="1">MMLMDTSLIGAINLTIHAFLVGSLLLGLHRKIMARIQGRPGPPIIQYLLHTLKFYVKEITFPITAGNPLYIFVALLDIAIWLAALIIAIDFKSSLLIIIGIYVLQKIVEHGCGLSSGSPYGKIGGVRSVFSAAAEVPLFAVVAAIYLTTHSVLISDILSYQEIHGSLLFKMPICAFAFFILLVSKAPNSPFGIVKGKDIVSGYMTEHYGLLGAIIYIAEAIAYFVLLWLFIAVFIGPLVINSPVLTLAVMVVMTVILAFVNGLTPLLAPHHSVMLQMTIAGLVLCDVLYRLIVGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="142" xmlns="http://uniprot.org/uniprot">
    <accession>Q57962</accession>
    <name>PPSA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable phosphoenolpyruvate synthase</fullName>
            <shortName>PEP synthase</shortName>
            <ecNumber>2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Pyruvate, water dikinase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mja pep intein</fullName>
            </recommendedName>
            <alternativeName>
                <fullName>Mja pepA intein</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name type="primary">ppsA</name>
        <name type="ordered locus">MJ0542</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + pyruvate = AMP + 2 H(+) + phosphate + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal domain contains the ATP/Pi binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site.</text>
    </comment>
    <comment type="PTM">
        <text evidence="4">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64367">
        <property type="entry name" value="F64367"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57962"/>
    <dbReference type="SMR" id="Q57962"/>
    <dbReference type="STRING" id="243232.MJ_0542"/>
    <dbReference type="EnsemblBacteria" id="AAB98534">
        <property type="protein sequence ID" value="AAB98534"/>
        <property type="gene ID" value="MJ_0542"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0542"/>
    <dbReference type="eggNOG" id="arCOG01111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007308_6_1_2"/>
    <dbReference type="InParanoid" id="Q57962"/>
    <dbReference type="OMA" id="RRFVQMY"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006090">
        <property type="term" value="P:pyruvate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PTHR43030"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="SSF52009"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000023556" description="Probable phosphoenolpyruvate synthase, 1st part" evidence="2">
        <location>
            <begin position="1"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000023557" description="Mja pep intein" evidence="2">
        <location>
            <begin position="411"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000023558" description="Probable phosphoenolpyruvate synthase, 2nd part" evidence="2">
        <location>
            <begin position="823"/>
            <end position="1188"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="3">
        <location>
            <begin position="536"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="824"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="1133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="917"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="964"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1061"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1061"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1083"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1084"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1085"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1086"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="1086"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="1188" mass="134012" checksum="5547E2E2A8C46AEC" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q57966</accession>
    <name>Y546_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0546</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0546</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64368">
        <property type="entry name" value="B64368"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57966"/>
    <dbReference type="SMR" id="Q57966"/>
    <dbReference type="STRING" id="243232.MJ_0546"/>
    <dbReference type="EnsemblBacteria" id="AAB98548">
        <property type="protein sequence ID" value="AAB98548"/>
        <property type="gene ID" value="MJ_0546"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0546"/>
    <dbReference type="eggNOG" id="arCOG05040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208223_0_0_2"/>
    <dbReference type="OMA" id="KVIMLQK"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106924" description="Uncharacterized protein MJ0546">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="56" mass="6460" checksum="66CBFC395548BA5D" modified="1996-11-01" version="1">MILYIIVAISILLNIILGIKVIMLQKELEEVKKATRLTKEEVEKLNERIRKLKLGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57976</accession>
    <name>M556_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methylated protein MJ0556</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0556</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Proc. Natl. Acad. Sci. U.S.A." volume="101" first="2678" last="2683">
            <title>Targeted analysis and discovery of posttranslational modifications in proteins from methanogenic archaea by top-down MS.</title>
            <authorList>
                <person name="Forbes A.J."/>
                <person name="Patrie S.M."/>
                <person name="Taylor G.K."/>
                <person name="Kim Y.-B."/>
                <person name="Jiang L."/>
                <person name="Kelleher N.L."/>
            </authorList>
            <dbReference type="PubMed" id="14976258"/>
            <dbReference type="DOI" id="10.1073/pnas.0306575101"/>
        </citation>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
        <scope>METHYLATION</scope>
    </reference>
    <comment type="PTM">
        <text evidence="2">Methylated at an undetermined residue between Ser-2 and Asp-26.</text>
    </comment>
    <comment type="mass spectrometry" mass="20207.0" method="Electrospray" evidence="2"/>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98550" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98550.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64369">
        <property type="entry name" value="D64369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496541.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57976"/>
    <dbReference type="SMR" id="Q57976"/>
    <dbReference type="STRING" id="243232.MJ_0556"/>
    <dbReference type="EnsemblBacteria" id="AAB98550">
        <property type="protein sequence ID" value="AAB98550"/>
        <property type="gene ID" value="MJ_0556"/>
    </dbReference>
    <dbReference type="GeneID" id="1451421"/>
    <dbReference type="KEGG" id="mja:MJ_0556"/>
    <dbReference type="eggNOG" id="arCOG02569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431673_0_0_2"/>
    <dbReference type="InParanoid" id="Q57976"/>
    <dbReference type="OMA" id="IIFMEDI"/>
    <dbReference type="OrthoDB" id="99405at2157"/>
    <dbReference type="PhylomeDB" id="Q57976"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="initiator methionine" description="Removed" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000106929" description="Methylated protein MJ0556">
        <location>
            <begin position="2"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="28"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="91"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="14976258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="174" mass="20352" checksum="984DEF1163F322ED" modified="2007-01-23" version="3">MSKFVKLHLVRTLNKYKELQKIRVKDVMISGDVIITTPEKTIKEIFDEMIKHNISGMPVVDDRGVMIGFITLREIRKYMTSHPYLNVGEVMLKNPPYTTADEDIITAFEKMIESNKKLDQLPVINTKYPEKILGKLEGIIFMEDIIKLLYENIIKELKTLVSFYNHNTEIKIKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q57991</accession>
    <name>AK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable aspartokinase</fullName>
            <ecNumber>2.7.2.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Aspartate kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0571</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-aspartate = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aspartokinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64371">
        <property type="entry name" value="C64371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870075.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2HMF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B/C/D=2-470"/>
    </dbReference>
    <dbReference type="PDB" id="3C1M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=1-473"/>
    </dbReference>
    <dbReference type="PDB" id="3C1N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.72 A"/>
        <property type="chains" value="A/B/C/D=1-473"/>
    </dbReference>
    <dbReference type="PDB" id="3C20">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1-473"/>
    </dbReference>
    <dbReference type="PDBsum" id="2HMF"/>
    <dbReference type="PDBsum" id="3C1M"/>
    <dbReference type="PDBsum" id="3C1N"/>
    <dbReference type="PDBsum" id="3C20"/>
    <dbReference type="AlphaFoldDB" id="Q57991"/>
    <dbReference type="SMR" id="Q57991"/>
    <dbReference type="STRING" id="243232.MJ_0571"/>
    <dbReference type="EnsemblBacteria" id="AAB98565">
        <property type="protein sequence ID" value="AAB98565"/>
        <property type="gene ID" value="MJ_0571"/>
    </dbReference>
    <dbReference type="GeneID" id="1451436"/>
    <dbReference type="KEGG" id="mja:MJ_0571"/>
    <dbReference type="eggNOG" id="arCOG00861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009116_6_0_2"/>
    <dbReference type="InParanoid" id="Q57991"/>
    <dbReference type="OMA" id="DMIVQTI"/>
    <dbReference type="OrthoDB" id="20371at2157"/>
    <dbReference type="PhylomeDB" id="Q57991"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-20452"/>
    <dbReference type="BRENDA" id="2.7.2.4">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57991"/>
    <dbReference type="PRO" id="PR:Q57991"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04244">
        <property type="entry name" value="AAK_AK-LysC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041746">
        <property type="entry name" value="AK-LysC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027795">
        <property type="entry name" value="CASTOR_ACT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13840">
        <property type="entry name" value="ACT_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000066688" description="Probable aspartokinase">
        <location>
            <begin position="1"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="ACT 1" evidence="1">
        <location>
            <begin position="323"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="ACT 2" evidence="1">
        <location>
            <begin position="409"/>
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    <evidence type="ECO:0000255" key="1">
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    </evidence>
    <evidence type="ECO:0000305" key="2"/>
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        <source>
            <dbReference type="PDB" id="2HMF"/>
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    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3C20"/>
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    <sequence length="473" mass="51392" checksum="811C6E0F4B66BC5F" modified="1996-11-01" version="1">MTTVMKFGGTSVGSGERIRHVAKIVTKRKKEDDDVVVVVSAMSEVTNALVEISQQALDVRDIAKVGDFIKFIREKHYKAIEEAIKSEEIKEEVKKIIDSRIEELEKVLIGVAYLGELTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPRLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITNDMEMSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETNISLVVSEEDVDKALKALKREFGDFGKKSFLNNNLIRDVSVDKDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKFIEKTNS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q58033</accession>
    <name>PURE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="1">N5-CAIR mutase</shortName>
            <ecNumber evidence="1">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purE</name>
        <name type="ordered locus">MJ0616</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64376">
        <property type="entry name" value="H64376"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870121.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2YWX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.31 A"/>
        <property type="chains" value="A=1-157"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="Q58033"/>
    <dbReference type="SMR" id="Q58033"/>
    <dbReference type="STRING" id="243232.MJ_0616"/>
    <dbReference type="PRIDE" id="Q58033"/>
    <dbReference type="EnsemblBacteria" id="AAB98611">
        <property type="protein sequence ID" value="AAB98611"/>
        <property type="gene ID" value="MJ_0616"/>
    </dbReference>
    <dbReference type="GeneID" id="1451482"/>
    <dbReference type="KEGG" id="mja:MJ_0616"/>
    <dbReference type="eggNOG" id="arCOG02464">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094982_2_0_2"/>
    <dbReference type="InParanoid" id="Q58033"/>
    <dbReference type="OMA" id="SNSIDGW"/>
    <dbReference type="OrthoDB" id="104083at2157"/>
    <dbReference type="PhylomeDB" id="Q58033"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58033"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000074987" description="N5-carboxyaminoimidazole ribonucleotide mutase">
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        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="strand" evidence="2">
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    </feature>
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        <location>
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    </feature>
    <feature type="helix" evidence="2">
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2YWX"/>
        </source>
    </evidence>
    <sequence length="157" mass="16951" checksum="398E2DDA35030078" modified="1996-11-01" version="1">MICIIMGSESDLKIAEKAVNILKEFGVEFEVRVASAHRTPELVEEIVKNSKADVFIAIAGLAAHLPGVVASLTTKPVIAVPVDAKLDGLDALLSSVQMPPGIPVATVGIDRGENAAILALEILALKDENIAKKLIEYREKMKKKVYASDEKVKEMFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58045</accession>
    <name>Y628_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0628</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0628</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0992.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64378">
        <property type="entry name" value="D64378"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870133.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58045"/>
    <dbReference type="SMR" id="Q58045"/>
    <dbReference type="STRING" id="243232.MJ_0628"/>
    <dbReference type="DNASU" id="1451494"/>
    <dbReference type="EnsemblBacteria" id="AAB98627">
        <property type="protein sequence ID" value="AAB98627"/>
        <property type="gene ID" value="MJ_0628"/>
    </dbReference>
    <dbReference type="GeneID" id="1451494"/>
    <dbReference type="KEGG" id="mja:MJ_0628"/>
    <dbReference type="eggNOG" id="arCOG06576">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114816_0_0_2"/>
    <dbReference type="OMA" id="GRYYYAS"/>
    <dbReference type="OrthoDB" id="112404at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106962" description="Uncharacterized protein MJ0628">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="166" mass="19743" checksum="4CFA7E0536F07C50" modified="1996-11-01" version="1">MGQMFNPLDFVYIAEFLEESKVDKKEAKNRTIIGRYYYASFLFLRGILKENLKNYNSKEAKEFLYLIELSNSHKIILDFLNVLKKEDGKFRRVYNALSILRDLRNASDYELESPARVKSIKEMVDFNDDYYVELSKNKYKIIVNSKSDVENILKDRSKIDKILRKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58075</accession>
    <name>Y661_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0661</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0661</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To H.pylori HP0274.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64382">
        <property type="entry name" value="E64382"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58075"/>
    <dbReference type="SMR" id="Q58075"/>
    <dbReference type="STRING" id="243232.MJ_0661"/>
    <dbReference type="EnsemblBacteria" id="AAB98654">
        <property type="protein sequence ID" value="AAB98654"/>
        <property type="gene ID" value="MJ_0661"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0661"/>
    <dbReference type="eggNOG" id="arCOG02579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521868_0_0_2"/>
    <dbReference type="InParanoid" id="Q58075"/>
    <dbReference type="OMA" id="KCKIYKV"/>
    <dbReference type="PhylomeDB" id="Q58075"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106978" description="Uncharacterized protein MJ0661">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="182" mass="21570" checksum="01E6F47F37008AFE" modified="1996-11-01" version="1">MESVRRGKLMKNKKIKFDVYLNGIAYHCIKCGFCCDAPTVTKKDLAKIAGYLKIPFDEVLKRYVRFFNGYIGELKEVGGKCIFLDKKTKKCKIYKVRPLICRLRPYSVQVRNGKLTLTYDIWFLRYCRGLYLGDGKVEDEYFKYAELVLKYLGFEEGVDEEEFKRAKERLLEESLKYRKKKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q58079</accession>
    <name>Y665_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0665</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0665</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64383">
        <property type="entry name" value="A64383"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58079"/>
    <dbReference type="SMR" id="Q58079"/>
    <dbReference type="STRING" id="243232.MJ_0665"/>
    <dbReference type="EnsemblBacteria" id="AAB98656">
        <property type="protein sequence ID" value="AAB98656"/>
        <property type="gene ID" value="MJ_0665"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0665"/>
    <dbReference type="eggNOG" id="arCOG01395">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067068_1_0_2"/>
    <dbReference type="InParanoid" id="Q58079"/>
    <dbReference type="OMA" id="NSSYCNG"/>
    <dbReference type="PhylomeDB" id="Q58079"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007152">
        <property type="entry name" value="DUF354"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39662">
        <property type="entry name" value="PTHR39662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04007">
        <property type="entry name" value="DUF354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005357">
        <property type="entry name" value="UCP005357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106981" description="Uncharacterized protein MJ0665">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <sequence length="341" mass="38789" checksum="DC678410BC847458" modified="1996-11-01" version="1">MINLDVWIDLTNAPHVHYFCQLIKKFEKEGIEYLLTFRDSGNLAKLVEIYNFVGKCIGKHGNTLKDKLIFYAERVIGLTELISNVKPKVAIAKHSVELPRVAFGLNIPVIFVVDNEHAEAQNKLTLPLADEIIKPIATDENKLKEFGGRNFISFEGTCEVANVNSRLKGYYPIDNEILKKLGICDDNPTIVMRPCPNSSYCNGHKDILPKIIEKLQKRIDCNIVVFPRDEHQKEIYREVNAIVPKETIDALSLLYNSDFMIGAGGTMNRESAILGIPTVSCYPQELLGVDKYLIEKDRMIHTNDIKEIINYVEDNLGKRMGVIELEDPTDLMFERVCNYLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58104</accession>
    <name>Y693_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0693</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0693</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64386">
        <property type="entry name" value="E64386"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58104"/>
    <dbReference type="SMR" id="Q58104"/>
    <dbReference type="STRING" id="243232.MJ_0693"/>
    <dbReference type="EnsemblBacteria" id="AAB98688">
        <property type="protein sequence ID" value="AAB98688"/>
        <property type="gene ID" value="MJ_0693"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0693"/>
    <dbReference type="eggNOG" id="arCOG01677">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1346434_0_0_2"/>
    <dbReference type="OMA" id="DMEPITP"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012028">
        <property type="entry name" value="UCP006529_dinclt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006529">
        <property type="entry name" value="UCP006529_dinclt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="SSF69572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106992" description="Uncharacterized protein MJ0693">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <sequence length="241" mass="27485" checksum="D183FD4DECBBFB8F" modified="1996-11-01" version="1">MNAFFEGVQCSLINFNNFAKNYYTFLLKKFCLDGIFMNVEEMERKLKPKGEVSIIGCGRLGVRVAFDLLEVHRGGVEKVYVFDNAKIEENDIVHRRLGGKVGEYKVDFIKRFFGNRVEAFRENITKDNLHLIKGDVAVICIAGGDTIPTTKAIINYCKERGIKTIGTNGVFGIEEKIKVCDAKYAKGPAKFLNLDEEGHIVVGTEKFIRDFEPITPYTLDEIAKRMVIECLRILWSKYYKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58120</accession>
    <name>TMG10_METJA</name>
    <protein>
        <recommendedName>
            <fullName>tRNA (guanine(10)-N2)-dimethyltransferase</fullName>
            <ecNumber>2.1.1.213</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>tRNA:G10 dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">trmG10</name>
        <name type="ordered locus">MJ0710</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N(2)) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) + N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10358"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.213"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyltransferase superfamily. Trm-G10 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.213"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64388">
        <property type="entry name" value="F64388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870216.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58120"/>
    <dbReference type="SMR" id="Q58120"/>
    <dbReference type="STRING" id="243232.MJ_0710"/>
    <dbReference type="EnsemblBacteria" id="AAB98704">
        <property type="protein sequence ID" value="AAB98704"/>
        <property type="gene ID" value="MJ_0710"/>
    </dbReference>
    <dbReference type="GeneID" id="1451578"/>
    <dbReference type="KEGG" id="mja:MJ_0710"/>
    <dbReference type="eggNOG" id="arCOG00047">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057819_1_0_2"/>
    <dbReference type="InParanoid" id="Q58120"/>
    <dbReference type="OMA" id="FFHPGVM"/>
    <dbReference type="OrthoDB" id="47556at2157"/>
    <dbReference type="PhylomeDB" id="Q58120"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005885">
        <property type="entry name" value="TrmG10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01177">
        <property type="entry name" value="TIGR01177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000140481" description="tRNA (guanine(10)-N2)-dimethyltransferase">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="57"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="351" mass="40483" checksum="4D0E17229D924E91" modified="1996-11-01" version="1">MIGYVLNGEHEEIPYGELMALLEIFNYNGSVERLKRYVITEDSPAKDIVKRSGYIDEGHRIIFRYNLEEKSVDLVDKIVNDFINSFKDFVANIDYPDIDESKSYAVRVLKLHKDEFTKSIDSLRIEKEIGGIIKLKTNAKVNLTKPDILVRVVILENTFFISNVLEMRDREYFQKNRPHLRKYFHPGCMLPKLARAMVNLARVKEGDIVLDPFCGTGGFLIEAGLIGAKLIGCDIDWRMASGTLINLEEYNLLDKVIKVKRLDAKYVKEFLNELNIEKVDAIVTDPPYGISTAKKGEIEKILETLPEVIKDNGYFVFAYPKKIELDMELEGLYKVYIHKGLIRHIHVYKKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q58133</accession>
    <name>PTR2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>HTH-type transcriptional regulator Ptr2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ptr2</name>
        <name type="ordered locus">MJ0723</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="EMBO J." volume="20" first="146" last="156">
            <title>A thermostable platform for transcriptional regulation: the DNA-binding properties of two Lrp homologs from the hyperthermophilic archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Ouhammouch M."/>
                <person name="Geiduschek E.P."/>
            </authorList>
            <dbReference type="PubMed" id="11226165"/>
            <dbReference type="DOI" id="10.1093/emboj/20.1.146"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="function">
        <text>Participates in positive as well as negative regulation of transcription. Binds to its own promoter.</text>
    </comment>
    <comment type="subunit">
        <text>Homotetramer.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98719" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98719.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64390">
        <property type="entry name" value="C64390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496594.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58133"/>
    <dbReference type="SMR" id="Q58133"/>
    <dbReference type="STRING" id="243232.MJ_0723"/>
    <dbReference type="EnsemblBacteria" id="AAB98719">
        <property type="protein sequence ID" value="AAB98719"/>
        <property type="gene ID" value="MJ_0723"/>
    </dbReference>
    <dbReference type="GeneID" id="1451600"/>
    <dbReference type="KEGG" id="mja:MJ_0723"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_4_2"/>
    <dbReference type="InParanoid" id="Q58133"/>
    <dbReference type="OMA" id="EDCWFIA"/>
    <dbReference type="OrthoDB" id="97695at2157"/>
    <dbReference type="PhylomeDB" id="Q58133"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000111753" description="HTH-type transcriptional regulator Ptr2">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="HTH asnC-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="140" mass="15903" checksum="B2135182FF834EC9" modified="2001-10-18" version="2">MDEKDLKIIEILMRDGRKSYTDIARELGTSESSIRKRVKKLEEEGVIKGYTAIIDPSKIGYNVVALTGFDTEPDKFLNVAKELCKFPEVKKVFTSTGDHMIMTEIWAKDGKEFSDLIFNKIGKIEGIKKICPAIILEQMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58161</accession>
    <name>Y751_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TnpB-like protein MJ0751</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0751</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64393">
        <property type="entry name" value="G64393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870256.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58161"/>
    <dbReference type="STRING" id="243232.MJ_0751"/>
    <dbReference type="PRIDE" id="Q58161"/>
    <dbReference type="EnsemblBacteria" id="AAB98747">
        <property type="protein sequence ID" value="AAB98747"/>
        <property type="gene ID" value="MJ_0751"/>
    </dbReference>
    <dbReference type="GeneID" id="1451628"/>
    <dbReference type="KEGG" id="mja:MJ_0751"/>
    <dbReference type="eggNOG" id="arCOG00680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1053140_0_0_2"/>
    <dbReference type="InParanoid" id="Q58161"/>
    <dbReference type="OMA" id="DWIAIDI"/>
    <dbReference type="OrthoDB" id="75656at2157"/>
    <dbReference type="PhylomeDB" id="Q58161"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0814">Transposable element</keyword>
    <keyword id="KW-0815">Transposition</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000107013" description="TnpB-like protein MJ0751">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="225"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q7DF80"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="278" mass="32990" checksum="B4EFAFF1893A58B3" modified="1997-11-01" version="1">MLFKFEGDKIKIITAPRKFITINLVVSDYQKKFIEEWKNGNFKIGEVIIKKDSIIIPFKKVVNPKNFEHIMTIDINEKNITYSIFDKDGNVIKTTRLDVYKLKRIHENFSKKREKIQKKLSNKPMKLKTLMEKYSGREKRKVEDYLHKISKFLISEALKYNVKILMEDLTNIREAVNKKSKNFRRRLNRWNFSKLQFFIEYKAKWDGLDVEYVNPSRTSKLCPICGCKLDPNGQRLLKCNNCNLVFDRDVVATFNLFKKSQDVGSFRSPERSLMKSSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58167</accession>
    <name>TMPS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Thymidylate synthase</fullName>
            <shortName>TS</shortName>
            <shortName>TSase</shortName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0757</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="Nat. Struct. Biol." volume="6" first="750" last="754">
            <title>Identifying two ancient enzymes in Archaea using predicted secondary structure alignment.</title>
            <authorList>
                <person name="Xu H."/>
                <person name="Aurora R."/>
                <person name="Rose G.D."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10426953"/>
            <dbReference type="DOI" id="10.1038/11525"/>
        </citation>
        <scope>FUNCTION AS A THYMIDYLATE SYNTHASE</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Is able to catalyze the biosynthesis of dTMP using dUMP, tetrahydrofolate and formaldehyde in vitro, i.e. a reaction equivalent to that catalyzed by bacterial thymidylate synthases (EC 2.1.1.45). However, M.jannaschii like most methanogenic Archaea lacks folates, thus the physiological cosubstrate is unknown but is likely one of the non-methylated methanopterin biosynthetic intermediates.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64394">
        <property type="entry name" value="E64394"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58167"/>
    <dbReference type="STRING" id="243232.MJ_0757"/>
    <dbReference type="PRIDE" id="Q58167"/>
    <dbReference type="DNASU" id="1451634"/>
    <dbReference type="EnsemblBacteria" id="AAB98749">
        <property type="protein sequence ID" value="AAB98749"/>
        <property type="gene ID" value="MJ_0757"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0757"/>
    <dbReference type="eggNOG" id="arCOG02468">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077076_0_0_2"/>
    <dbReference type="OMA" id="HHSYPCT"/>
    <dbReference type="PhylomeDB" id="Q58167"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14571"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107016" description="Thymidylate synthase">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="10426953"/>
        </source>
    </evidence>
    <sequence length="260" mass="30434" checksum="F0BBD83A4143BF08" modified="1996-11-01" version="1">MVLNINSFYKNCRGEFMRAVFIYHKNNQRMEKFYKNLLNEPDFCRICDDCYNCRGNWTFKNNVKNIVIEEVYEEFVDNPYDYLPELPEGDICIAQLHEDLLYELPLLLKEKGYKALIVPSETPHDLSLALRRDLKRVCSNYNIEFENPKPFCSLEKKEGNEYINKFIDYFKIGKPELEIEVENGLIKDVKVKISAPCGETYYIAKRLKGKAIDDLKEEIANAHHNYPCLASMEMDKELGDTILHKAGYIAFEVVEKALKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="144" xmlns="http://uniprot.org/uniprot">
    <accession>Q58192</accession>
    <name>TF2B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Transcription initiation factor IIB</fullName>
            <shortName>TFIIB</shortName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Endonuclease Mja Tfb</fullName>
                <ecNumber>3.1.-.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Mja TFIIB intein</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Mja Tfb intein</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name type="primary">tfb</name>
        <name type="ordered locus">MJ0782</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64397">
        <property type="entry name" value="F64397"/>
    </dbReference>
    <dbReference type="PDB" id="5O9J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=101-225, A/B=356-435"/>
    </dbReference>
    <dbReference type="PDBsum" id="5O9J"/>
    <dbReference type="AlphaFoldDB" id="Q58192"/>
    <dbReference type="SMR" id="Q58192"/>
    <dbReference type="STRING" id="243232.MJ_0782"/>
    <dbReference type="MEROPS" id="N10.007"/>
    <dbReference type="PRIDE" id="Q58192"/>
    <dbReference type="EnsemblBacteria" id="AAB98771">
        <property type="protein sequence ID" value="AAB98771"/>
        <property type="gene ID" value="MJ_0782"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0782"/>
    <dbReference type="eggNOG" id="arCOG01981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026356_0_0_2"/>
    <dbReference type="InParanoid" id="Q58192"/>
    <dbReference type="OMA" id="HNTIHDK"/>
    <dbReference type="OrthoDB" id="11535at2157"/>
    <dbReference type="PhylomeDB" id="Q58192"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00043">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="PTHR11618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="TF_Zn_Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="SSF47954"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0404">Intron homing</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000033642" description="Transcription initiation factor IIB, 1st part" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000033643" description="Endonuclease Mja Tfb" evidence="1">
        <location>
            <begin position="100"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000033644" description="Transcription initiation factor IIB, 2nd part" evidence="1">
        <location>
            <begin position="435"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease">
        <location>
            <begin position="238"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="490"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="584"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TFIIB-type" evidence="2">
        <location>
            <begin position="38"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="106"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="114"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="140"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="146"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="159"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="166"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="175"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="181"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="184"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="192"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="201"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="207"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="215"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="394"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="408"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="418"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="422"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="431"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="5O9J"/>
        </source>
    </evidence>
    <sequence length="673" mass="76969" checksum="20EA4713EAB031A9" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58204</accession>
    <name>Y794_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0794</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0794</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64399">
        <property type="entry name" value="B64399"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58204"/>
    <dbReference type="SMR" id="Q58204"/>
    <dbReference type="STRING" id="243232.MJ_0794"/>
    <dbReference type="EnsemblBacteria" id="AAB98797">
        <property type="protein sequence ID" value="AAB98797"/>
        <property type="gene ID" value="MJ_0794"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0794"/>
    <dbReference type="eggNOG" id="arCOG02054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1821030_0_0_2"/>
    <dbReference type="InParanoid" id="Q58204"/>
    <dbReference type="OMA" id="ALWSANI"/>
    <dbReference type="PhylomeDB" id="Q58204"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006977">
        <property type="entry name" value="Yip1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04893">
        <property type="entry name" value="Yip1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107045" description="Uncharacterized protein MJ0794">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="141" mass="15935" checksum="39CD0EA926BDC08C" modified="1996-11-01" version="1">MILMNLIEALTNPDTFFKKLSQKEISLKEPFLIVLIFSILIAISAYISTSIIYKIFPPQYQQVLAFTKIIALISTFIGGIVAWLIIAGFMHLISMIFKGEGSFKKTLSFTGYGFLPNIVGALITIPIAYYMREISQQEMRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58227</accession>
    <name>ASD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative archaetidylserine decarboxylase proenzyme</fullName>
            <ecNumber evidence="1">4.1.1.-</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="1">Archaetidylserine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="1">Archaetidylserine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">asd</name>
        <name type="ordered locus">MJ0817</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of archaetidylethanolamine (PtdEtn) from archaetidylserine (PtdSer).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>archaetidylserine + H(+) = archaetidylethanolamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:51488"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:71517"/>
            <dbReference type="ChEBI" id="CHEBI:134176"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="1">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64402">
        <property type="entry name" value="A64402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870328.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58227"/>
    <dbReference type="STRING" id="243232.MJ_0817"/>
    <dbReference type="EnsemblBacteria" id="AAB98815">
        <property type="protein sequence ID" value="AAB98815"/>
        <property type="gene ID" value="MJ_0817"/>
    </dbReference>
    <dbReference type="GeneID" id="1451700"/>
    <dbReference type="KEGG" id="mja:MJ_0817"/>
    <dbReference type="eggNOG" id="arCOG04470">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072492_1_0_2"/>
    <dbReference type="InParanoid" id="Q58227"/>
    <dbReference type="OMA" id="VSIFMSP"/>
    <dbReference type="OrthoDB" id="105037at2157"/>
    <dbReference type="PhylomeDB" id="Q58227"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00664">
        <property type="entry name" value="PS_decarb_PSD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033175">
        <property type="entry name" value="PSD-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35809">
        <property type="entry name" value="PTHR35809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00164">
        <property type="entry name" value="PS_decarb_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_0000029825" description="Archaetidylserine decarboxylase beta chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000029826" description="Archaetidylserine decarboxylase alpha chain" evidence="1">
        <location>
            <begin position="172"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autocatalysis" evidence="1">
        <location>
            <begin position="171"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00664"/>
        </source>
    </evidence>
    <sequence length="206" mass="23055" checksum="45A264987E23FCBF" modified="1996-11-01" version="1">MGKYKKFFAIAVCSLLLFTVYFYRDPDRVITKGNNIILSPADGTVEYIKFYENGNPEVFKDGNCYVLNVSRYFPNGCYVVGIFMSPLDVHVNRAPIGGRIVYIKHIDGSFYPAFLEGVEKINERNIVIIKNGSEYVGVVQIAGFVARRCWLSIKEGECINMGQKIGMIKLGSQTAVIIPANYNITVKVGERVYAGQTIIAVKRTDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58269</accession>
    <name>Y859_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0859</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0859</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64407">
        <property type="entry name" value="C64407"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58269"/>
    <dbReference type="SMR" id="Q58269"/>
    <dbReference type="STRING" id="243232.MJ_0859"/>
    <dbReference type="EnsemblBacteria" id="AAB98874">
        <property type="protein sequence ID" value="AAB98874"/>
        <property type="gene ID" value="MJ_0859"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0859"/>
    <dbReference type="eggNOG" id="arCOG01724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1736436_0_0_2"/>
    <dbReference type="InParanoid" id="Q58269"/>
    <dbReference type="OMA" id="MKIPKIY"/>
    <dbReference type="PhylomeDB" id="Q58269"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019217">
        <property type="entry name" value="DUF2118"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09891">
        <property type="entry name" value="DUF2118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019115">
        <property type="entry name" value="UCP019115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107084" description="Uncharacterized protein MJ0859">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <sequence length="164" mass="18986" checksum="D1C3930A857C2366" modified="1996-11-01" version="1">MNIMRIPRLYVENAEKHEGRKVVIENGGKVIKFLDKDEEYEGDGKVLYQVIYDDFDNYVLMGTVTKDMIIEYEVGGVRQITYIKKGTKLLEIPAEGYKVYPIVDFGCRILGGHRIAALQSRKGDIRFVNTPVNGIVLFLKEVPAKRENYVFYILPEEEIKFEEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58272</accession>
    <name>IDI2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isopentenyl-diphosphate delta-isomerase</fullName>
            <shortName evidence="1">IPP isomerase</shortName>
            <ecNumber evidence="1">5.3.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl diphosphate:dimethylallyl diphosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Isopentenyl pyrophosphate isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type 2 isopentenyl diphosphate isomerase</fullName>
            <shortName evidence="1">IDI-2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fni</name>
        <name type="ordered locus">MJ0862</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Bioorg. Med. Chem." volume="14" first="6555" last="6559">
            <title>Inhibition of type 2 isopentenyl diphosphate isomerase from Methanocaldococcus jannaschii by a mechanism-based inhibitor of type 1 isopentenyl diphosphate isomerase.</title>
            <authorList>
                <person name="Hoshino T."/>
                <person name="Tamegai H."/>
                <person name="Kakinuma K."/>
                <person name="Eguchi T."/>
            </authorList>
            <dbReference type="PubMed" id="16793276"/>
            <dbReference type="DOI" id="10.1016/j.bmc.2006.06.011"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>COFACTOR</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="Acta Crystallogr. F Struct. Biol. Commun." volume="67" first="101" last="103">
            <title>Crystallization and preliminary X-ray analysis of isopentenyl diphosphate isomerase from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Hoshino T."/>
                <person name="Nango E."/>
                <person name="Baba S."/>
                <person name="Eguchi T."/>
                <person name="Kumasaka T."/>
            </authorList>
            <dbReference type="PubMed" id="21206036"/>
            <dbReference type="DOI" id="10.1107/S1744309110046944"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Involved in the biosynthesis of isoprenoids. Catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its allylic isomer, dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>isopentenyl diphosphate = dimethylallyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23284"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="5.3.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by 3,4-epoxy-3-methylbutyl diphosphate (EIPP).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">15.3 mM for IPP</KM>
            <text>kcat is 191 sec(-1) for isomerase activity with IPP.</text>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is between 7 and 7.2.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is between 85 and 95 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer. Dimer of tetramers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IPP isomerase type 2 family.</text>
    </comment>
    <dbReference type="EC" id="5.3.3.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64407">
        <property type="entry name" value="F64407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870377.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58272"/>
    <dbReference type="SMR" id="Q58272"/>
    <dbReference type="STRING" id="243232.MJ_0862"/>
    <dbReference type="EnsemblBacteria" id="AAB98867">
        <property type="protein sequence ID" value="AAB98867"/>
        <property type="gene ID" value="MJ_0862"/>
    </dbReference>
    <dbReference type="GeneID" id="1451751"/>
    <dbReference type="KEGG" id="mja:MJ_0862"/>
    <dbReference type="eggNOG" id="arCOG00613">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065515_1_0_2"/>
    <dbReference type="InParanoid" id="Q58272"/>
    <dbReference type="OMA" id="WDWGIPT"/>
    <dbReference type="OrthoDB" id="42330at2157"/>
    <dbReference type="PhylomeDB" id="Q58272"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-21126"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004452">
        <property type="term" value="F:isopentenyl-diphosphate delta-isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070402">
        <property type="term" value="F:NADPH binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02811">
        <property type="entry name" value="IDI-2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00354">
        <property type="entry name" value="Idi_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000262">
        <property type="entry name" value="FMN-dep_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011179">
        <property type="entry name" value="IPdP_isomerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43665">
        <property type="entry name" value="PTHR43665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01070">
        <property type="entry name" value="FMN_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003314">
        <property type="entry name" value="IPP_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02151">
        <property type="entry name" value="IPP_isom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134444" description="Isopentenyl-diphosphate delta-isomerase">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="69"/>
            <end position="71"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="99"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="278"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="299"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16793276"/>
        </source>
    </evidence>
    <sequence length="359" mass="39875" checksum="46BDD320262772EA" modified="1996-11-01" version="1">MVNNRNEIEVRKLEHIFLCSYCNVEYEKTTLLEDIELIHKGTCGINFNDIETEIELFGKKLSAPIIVSGMTGGHSKAKEINKNIAKAVEELGLGMGVGSQRAAIVNDELIDTYSIVRDYTNNLVIGNLGAVNFIVDDWDEEIIDKAIEMIDADAIAIHFNPLQEIIQPEGDLNFKNLYKLKEIISNYKKSYKNIPFIAKQVGEGFSKEDALILKDIGFDAIDVQGSGGTSWAKVEIYRVKEEEIKRLAEKFANWGIPTAASIFEVKSVYDGIVIGSGGIRGGLDIAKCIAIGCDCCSVALPILKASLKGWEEVVKVLESYIKELKIAMFLVGAENIEELKKTSYIVKGTLKEWISQRLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58302</accession>
    <name>FLAB2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Flagellin B2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaB2</name>
        <name type="ordered locus">MJ0892</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64411">
        <property type="entry name" value="D64411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870406.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58302"/>
    <dbReference type="SMR" id="Q58302"/>
    <dbReference type="STRING" id="243232.MJ_0892"/>
    <dbReference type="EnsemblBacteria" id="AAB98895">
        <property type="protein sequence ID" value="AAB98895"/>
        <property type="gene ID" value="MJ_0892"/>
    </dbReference>
    <dbReference type="GeneID" id="1451781"/>
    <dbReference type="KEGG" id="mja:MJ_0892"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051124_0_1_2"/>
    <dbReference type="InParanoid" id="Q58302"/>
    <dbReference type="OMA" id="IVEIQDA"/>
    <dbReference type="OrthoDB" id="94462at2157"/>
    <dbReference type="PhylomeDB" id="Q58302"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="PTHR35903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Arch_flagellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="propeptide" id="PRO_0000009375" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000009376" description="Flagellin B2">
        <location>
            <begin position="13"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="217" mass="22577" checksum="6E9D9435C243A82D" modified="1997-11-01" version="1" precursor="true">MKVFEFLKGKRGAMGIGTLIIFIAMVLVAAVAAAVLINTSGFLQQKAMATGKESTEQVASGLSTLQVIGIHDNKAINYLAIYITPNAGSAAIDLNQTKILITDGEKKAVLRYNSNAYADLTTGGEVTNTSLAAWNLSGGEFGIIVLQDADGSCKSTTPVINKGDIVALTINASAVGLNLVPRTTVTGSVIPEFGAPAVIEFTTPAAYLSTQEVIQLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58309</accession>
    <name>FLAH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative flagella-related protein H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaH</name>
        <name type="ordered locus">MJ0899</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.voltae FlaH.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64412">
        <property type="entry name" value="C64412"/>
    </dbReference>
    <dbReference type="PDB" id="4WIA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B/C=1-233"/>
    </dbReference>
    <dbReference type="PDBsum" id="4WIA"/>
    <dbReference type="AlphaFoldDB" id="Q58309"/>
    <dbReference type="SMR" id="Q58309"/>
    <dbReference type="STRING" id="243232.MJ_0899"/>
    <dbReference type="EnsemblBacteria" id="AAB98902">
        <property type="protein sequence ID" value="AAB98902"/>
        <property type="gene ID" value="MJ_0899"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0899"/>
    <dbReference type="eggNOG" id="arCOG04148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094838_0_0_2"/>
    <dbReference type="InParanoid" id="Q58309"/>
    <dbReference type="OMA" id="IVKYNNA"/>
    <dbReference type="PhylomeDB" id="Q58309"/>
    <dbReference type="BRENDA" id="3.6.4.B6">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000087278" description="Putative flagella-related protein H">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="22"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="29"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="40"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="58"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="82"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="89"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="108"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="116"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="121"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="129"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="158"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="165"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="170"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="181"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="194"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="213"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="224"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4WIA"/>
        </source>
    </evidence>
    <sequence length="233" mass="26010" checksum="6225E2B2E8DD22CA" modified="1996-11-01" version="1">MGIMELARIDLSRDDLDKRIGGGIPHGSLIIIEGEESTGKSVLCQRLAYGFLQNRYSVTYVSTQLTTLEFIKQMNSLNYSINKKLLSGALLYIPVYPLIADNKKKDGFLKKVMETRAFYEKDVIIFDSISALIANDASEVNVDDLMAFFKRITALKKIIICTVNPKELPESVLTIIRTSATMLIRTELFTFGGDLKNLAKILKYNMAPGSYQKNIVFRVEPKIGIAVEIASVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58335</accession>
    <name>Y925_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0925</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0925</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64415">
        <property type="entry name" value="E64415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870439.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58335"/>
    <dbReference type="SMR" id="Q58335"/>
    <dbReference type="STRING" id="243232.MJ_0925"/>
    <dbReference type="EnsemblBacteria" id="AAB98937">
        <property type="protein sequence ID" value="AAB98937"/>
        <property type="gene ID" value="MJ_0925"/>
    </dbReference>
    <dbReference type="GeneID" id="1451814"/>
    <dbReference type="KEGG" id="mja:MJ_0925"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2217109_0_0_2"/>
    <dbReference type="InParanoid" id="Q58335"/>
    <dbReference type="OMA" id="KWEAKWK"/>
    <dbReference type="OrthoDB" id="112375at2157"/>
    <dbReference type="PhylomeDB" id="Q58335"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000148893" description="Uncharacterized HTH-type transcriptional regulator MJ0925">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="HTH hxlR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00435"/>
        </source>
    </evidence>
    <sequence length="106" mass="12115" checksum="09ADFAF2CA628223" modified="1996-11-01" version="1">MSIFYVLGKKGTIEILYKIKEGVNSFTSIKNALDMEGCGVSTRTLAERLNELEDENLIQKDGSKYYLTKKGQEALEIIENVMKWEAKWKEAKIPKIIIGMLGDKER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58370</accession>
    <name>TAL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable transaldolase</fullName>
            <ecNumber>2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">tal</name>
        <name type="ordered locus">MJ0960</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = beta-D-fructose 6-phosphate + D-erythrose 4-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transaldolase family. Type 3B subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64419">
        <property type="entry name" value="H64419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870474.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58370"/>
    <dbReference type="SMR" id="Q58370"/>
    <dbReference type="STRING" id="243232.MJ_0960"/>
    <dbReference type="EnsemblBacteria" id="AAB98962">
        <property type="protein sequence ID" value="AAB98962"/>
        <property type="gene ID" value="MJ_0960"/>
    </dbReference>
    <dbReference type="GeneID" id="1451858"/>
    <dbReference type="KEGG" id="mja:MJ_0960"/>
    <dbReference type="eggNOG" id="arCOG05061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079764_0_0_2"/>
    <dbReference type="InParanoid" id="Q58370"/>
    <dbReference type="OMA" id="VRHPMHV"/>
    <dbReference type="OrthoDB" id="77186at2157"/>
    <dbReference type="PhylomeDB" id="Q58370"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00956">
        <property type="entry name" value="Transaldolase_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00494">
        <property type="entry name" value="Transaldolase_3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022999">
        <property type="entry name" value="Transaldolase_3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004731">
        <property type="entry name" value="Transaldolase_3B/F6P_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033919">
        <property type="entry name" value="TSA/FSA_arc/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="PTHR10683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00875">
        <property type="entry name" value="fsa_talC_mipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0570">Pentose shunt</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000173689" description="Probable transaldolase">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="217" mass="23960" checksum="E2FEDF98DDDA2F82" modified="1996-11-01" version="1">MKFFLDTANVEEIKKYAELGLVDGVTTNPTLVAKEGRDFYEVVKEICEIVEGPVSAEVISTDAEGMVKEARELAKLADNIVIKIPMTKDGMKAVKILSAEGIKTNVTLVFSPLQALVAAKAGATYVSPFVGRLDDIGHVGMKLIEDVVKIYKNYDIKTEVIVASVRHPWHVLEAAKIGADIATMPPAVMDKLFNHPLTDIGLERFLKDWDEYLKSRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58373</accession>
    <name>Y963_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0963</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0963</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HyuB family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64420">
        <property type="entry name" value="C64420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870477.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58373"/>
    <dbReference type="SMR" id="Q58373"/>
    <dbReference type="STRING" id="243232.MJ_0963"/>
    <dbReference type="PRIDE" id="Q58373"/>
    <dbReference type="EnsemblBacteria" id="AAB98965">
        <property type="protein sequence ID" value="AAB98965"/>
        <property type="gene ID" value="MJ_0963"/>
    </dbReference>
    <dbReference type="GeneID" id="1451861"/>
    <dbReference type="KEGG" id="mja:MJ_0963"/>
    <dbReference type="eggNOG" id="arCOG01512">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020413_0_0_2"/>
    <dbReference type="InParanoid" id="Q58373"/>
    <dbReference type="OMA" id="QVGMTNT"/>
    <dbReference type="OrthoDB" id="4422at2157"/>
    <dbReference type="PhylomeDB" id="Q58373"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003692">
        <property type="entry name" value="Hydantoinase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045079">
        <property type="entry name" value="Oxoprolinase_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11365">
        <property type="entry name" value="PTHR11365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02538">
        <property type="entry name" value="Hydantoinase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000208585" description="Uncharacterized protein MJ0963">
        <location>
            <begin position="1"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="563" mass="62148" checksum="7D92D7DEAC98FE7F" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58389</accession>
    <name>Y979_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0979</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0979</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64422">
        <property type="entry name" value="C64422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870493.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58389"/>
    <dbReference type="EnsemblBacteria" id="AAB98994">
        <property type="protein sequence ID" value="AAB98994"/>
        <property type="gene ID" value="MJ_0979"/>
    </dbReference>
    <dbReference type="GeneID" id="1451877"/>
    <dbReference type="KEGG" id="mja:MJ_0979"/>
    <dbReference type="eggNOG" id="arCOG14832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1381407_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107128" description="Uncharacterized protein MJ0979">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="197" mass="21520" checksum="B250A41C03D10A3E" modified="1996-11-01" version="1">MLPKKIDYIKIALIVVGIIALFLPWLTISASTINIKTDEGIHLSVNLAPFRVSSDIKSDTNNIFVEMMMPYVKQYFDMAVKEKMSTFMMIFGIIPIILYIASIFVDKKAVVVGAGIAGITCASIFVVLFTVGLNSSDSGLALTGGKEVTPIDLITGVVNEKSSYLSKDIIKIQVGTGWYLTMIIGLALIAYPFIRKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58410</accession>
    <name>Y1004_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1004</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1004</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64425">
        <property type="entry name" value="C64425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870517.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6H1W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.39 A"/>
        <property type="chains" value="A/B=1-214"/>
    </dbReference>
    <dbReference type="PDBsum" id="6H1W"/>
    <dbReference type="AlphaFoldDB" id="Q58410"/>
    <dbReference type="SMR" id="Q58410"/>
    <dbReference type="STRING" id="243232.MJ_1004"/>
    <dbReference type="EnsemblBacteria" id="AAB99008">
        <property type="protein sequence ID" value="AAB99008"/>
        <property type="gene ID" value="MJ_1004"/>
    </dbReference>
    <dbReference type="GeneID" id="1451901"/>
    <dbReference type="KEGG" id="mja:MJ_1004"/>
    <dbReference type="eggNOG" id="arCOG00628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1286363_0_0_2"/>
    <dbReference type="OMA" id="AIWACGG"/>
    <dbReference type="OrthoDB" id="61989at2157"/>
    <dbReference type="PhylomeDB" id="Q58410"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016486">
        <property type="entry name" value="UCP006591_CBS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006591">
        <property type="entry name" value="UCP006591_CBS_MJ1004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000107139" description="Uncharacterized protein MJ1004">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="69"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="3"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="33"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="42"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="65"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="99"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="106"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="114"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="123"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="166"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="178"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="206"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="6H1W"/>
        </source>
    </evidence>
    <sequence length="214" mass="24585" checksum="7D259934229043E7" modified="1996-11-01" version="1">MKVRDLMDKNFAKIYVDETVEDAINLLKKKKRFSAPIVDKEDRLVGWVTTLELLGISEKDFKKPITEFMRPVEEVITVYEDDEARNVVLKFVKYKVVSIPVLTRDGRVIGMVRNCDVVKTLAKLYEIPVYKIFKELHNHIGDISWEELMEAAAVVTKRMTGEDITPQEYEERIKKTTFGKAIWACGGLEKFFVGLIEIGMVALARKLAKRRKGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58423</accession>
    <name>Y1017_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1017</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1017</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64426">
        <property type="entry name" value="H64426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870530.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58423"/>
    <dbReference type="STRING" id="243232.MJ_1017"/>
    <dbReference type="EnsemblBacteria" id="AAB99023">
        <property type="protein sequence ID" value="AAB99023"/>
        <property type="gene ID" value="MJ_1017"/>
    </dbReference>
    <dbReference type="GeneID" id="1451914"/>
    <dbReference type="KEGG" id="mja:MJ_1017"/>
    <dbReference type="eggNOG" id="arCOG03518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318547_0_0_2"/>
    <dbReference type="OMA" id="PIFWIFL"/>
    <dbReference type="OrthoDB" id="83788at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
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    </dbReference>
    <dbReference type="GO" id="GO:0016021">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107143" description="Uncharacterized protein MJ1017">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="14"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="203" mass="23749" checksum="DA69129BA5C2CA1D" modified="1996-11-01" version="1">MKVKVYRVGGITGIFLIFLILLLLIILAIVALPIFLILMAIFGGYILLKYKIKSFFRKVWYNIRKKKIKIEDTSTNGEVKINFAKRIEIEDGKIETNNINTLLDYLDENTKSFAYYLKNIGAEFRDDGIYFKGYKIYPIFKKSYPINEIISLRYPENIDAVVLGLKGEPYDPKFLYLIPKEFLKDRMSISELKRFEIGDMVLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-12-13" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58435</accession>
    <name>RSMA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rsmA</name>
        <name evidence="1" type="synonym">ksgA</name>
        <name type="ordered locus">MJ1029</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="J. Mol. Biol." volume="391" first="884" last="893">
            <title>Structural and functional divergence within the Dim1/KsgA family of rRNA methyltransferases.</title>
            <authorList>
                <person name="Pulicherla N."/>
                <person name="Pogorzala L.A."/>
                <person name="Xu Z."/>
                <person name="O'Farrell H.C."/>
                <person name="Musayev F.N."/>
                <person name="Scarsdale J.N."/>
                <person name="Sia E.A."/>
                <person name="Culver G.M."/>
                <person name="Rife J.P."/>
            </authorList>
            <dbReference type="PubMed" id="19520088"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2009.06.015"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="Biochemistry" volume="49" first="2697" last="2704">
            <title>Binding of adenosine-based ligands to the MjDim1 rRNA methyltransferase: implications for reaction mechanism and drug design.</title>
            <authorList>
                <person name="O'Farrell H.C."/>
                <person name="Musayev F.N."/>
                <person name="Scarsdale J.N."/>
                <person name="Rife J.P."/>
            </authorList>
            <dbReference type="PubMed" id="20163168"/>
            <dbReference type="DOI" id="10.1021/bi901875x"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) IN COMPLEXES WITH AMP; S-ADENOSYL-L-HOMOCYSTEINE; S-ADENOSYL-L-METHIONINE AND ADENOSINE</scope>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64428">
        <property type="entry name" value="D64428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870542.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="PDB" id="3FYC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.15 A"/>
        <property type="chains" value="A/B=10-272"/>
    </dbReference>
    <dbReference type="PDB" id="3FYD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A=10-272"/>
    </dbReference>
    <dbReference type="PDB" id="3GRR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=1-275"/>
    </dbReference>
    <dbReference type="PDB" id="3GRU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-275"/>
    </dbReference>
    <dbReference type="PDB" id="3GRV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-275"/>
    </dbReference>
    <dbReference type="PDB" id="3GRY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=1-275"/>
    </dbReference>
    <dbReference type="PDBsum" id="3FYC"/>
    <dbReference type="PDBsum" id="3FYD"/>
    <dbReference type="PDBsum" id="3GRR"/>
    <dbReference type="PDBsum" id="3GRU"/>
    <dbReference type="PDBsum" id="3GRV"/>
    <dbReference type="PDBsum" id="3GRY"/>
    <dbReference type="AlphaFoldDB" id="Q58435"/>
    <dbReference type="SMR" id="Q58435"/>
    <dbReference type="STRING" id="243232.MJ_1029"/>
    <dbReference type="EnsemblBacteria" id="AAB99033">
        <property type="protein sequence ID" value="AAB99033"/>
        <property type="gene ID" value="MJ_1029"/>
    </dbReference>
    <dbReference type="GeneID" id="1451926"/>
    <dbReference type="KEGG" id="mja:MJ_1029"/>
    <dbReference type="eggNOG" id="arCOG04131">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041220_0_2_2"/>
    <dbReference type="InParanoid" id="Q58435"/>
    <dbReference type="OMA" id="KEEEPYF"/>
    <dbReference type="OrthoDB" id="99734at2157"/>
    <dbReference type="PhylomeDB" id="Q58435"/>
    <dbReference type="EvolutionaryTrace" id="Q58435"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="PTHR11727"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000101656" description="Probable ribosomal RNA small subunit methyltransferase A">
        <location>
            <begin position="1"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="33"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="43"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="52"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
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            <end position="64"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="65"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="76"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="85"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="96"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
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            <begin position="104"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="120"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="128"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="145"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="154"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="167"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="175"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="204"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="210"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="218"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="225"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="240"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="250"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="255"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19520088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="20163168"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3GRU"/>
        </source>
    </evidence>
    <sequence length="275" mass="31771" checksum="BB4DD454BD36427A" modified="1996-11-01" version="1">MFKPKKKLGQCFLIDKNFVNKAVESANLTKDDVVLEIGLGKGILTEELAKNAKKVYVIEIDKSLEPYANKLKELYNNIEIIWGDALKVDLNKLDFNKVVANLPYQISSPITFKLIKRGFDLAVLMYQYEFAKRMVAKEGTKDYGRLSVAVQSRADVEIVAKVPPSAFYPKPKVYSAIVKIKPNKGKYHIENENFFDDFLRAIFQHRNKSVRKALIDSSKELNYNKDEMKKILEDFLNTNSEIKNLINEKVFKLSVKDIVNLSNEFYRFLQNRGRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58441</accession>
    <name>MTD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>F420-dependent methylenetetrahydromethanopterin dehydrogenase</fullName>
            <shortName>MTD</shortName>
            <ecNumber>1.5.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtd</name>
        <name type="ordered locus">MJ1035</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5,10-methylenetetrahydromethanopterin + 2 H(+) + oxidized coenzyme F420-(gamma-L-Glu)(n) = 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:16721"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MTD family.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64429">
        <property type="entry name" value="B64429"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870548.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58441"/>
    <dbReference type="SMR" id="Q58441"/>
    <dbReference type="STRING" id="243232.MJ_1035"/>
    <dbReference type="EnsemblBacteria" id="AAB99039">
        <property type="protein sequence ID" value="AAB99039"/>
        <property type="gene ID" value="MJ_1035"/>
    </dbReference>
    <dbReference type="GeneID" id="1451932"/>
    <dbReference type="KEGG" id="mja:MJ_1035"/>
    <dbReference type="eggNOG" id="arCOG04382">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1006890_0_0_2"/>
    <dbReference type="InParanoid" id="Q58441"/>
    <dbReference type="OMA" id="KGCFMTK"/>
    <dbReference type="OrthoDB" id="46875at2157"/>
    <dbReference type="PhylomeDB" id="Q58441"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00695"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030268">
        <property type="term" value="F:methylenetetrahydromethanopterin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00058">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002844">
        <property type="entry name" value="MTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036080">
        <property type="entry name" value="MTD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01993">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005627">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102324">
        <property type="entry name" value="SSF102324"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000075038" description="F420-dependent methylenetetrahydromethanopterin dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="252"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="277" mass="30306" checksum="93A1D2357CC85DDF" modified="1996-11-01" version="1">MVVKIGIIKCGNIGMSPVVDLALDERADRKDIAVRVLGSGAKMDPESVEEVTKKMVEEVKPDFIIYIGPNPAAPGPKKAREILSQSGIPAVIIGDAPGLRVKDEMEQQGLGYIIIKCDPMIGARREFLDPVEMALFNADVIRVLAGTGALRIVQEAIDKMIDAVKEGKEIELPKIVITEQKAVEAMEFTNPYAKAKAMAAFTIAEKVGDVDVKGCFMTKEAEKYIPIVASAHEMIRYAAKLVDEARELEKAMDAVSRKPHHPEGKRLSKKALMEKPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58475</accession>
    <name>TRPE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Anthranilate synthase component 1</fullName>
            <shortName>AS</shortName>
            <shortName>ASI</shortName>
            <ecNumber>4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpE</name>
        <name type="ordered locus">MJ1075</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>chorismate + L-glutamine = anthranilate + H(+) + L-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Feedback inhibited by tryptophan.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64434">
        <property type="entry name" value="B64434"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58475"/>
    <dbReference type="SMR" id="Q58475"/>
    <dbReference type="STRING" id="243232.MJ_1075"/>
    <dbReference type="EnsemblBacteria" id="AAB99074">
        <property type="protein sequence ID" value="AAB99074"/>
        <property type="gene ID" value="MJ_1075"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1075"/>
    <dbReference type="eggNOG" id="arCOG02014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006493_9_3_2"/>
    <dbReference type="InParanoid" id="Q58475"/>
    <dbReference type="OMA" id="GCVGYLD"/>
    <dbReference type="PhylomeDB" id="Q58475"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010116">
        <property type="entry name" value="Anthranilate_synth_I_arc_typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="PTHR11236"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="SSF56322"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01820">
        <property type="entry name" value="TrpE-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154128" description="Anthranilate synthase component 1">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="254"/>
            <end position="256"/>
        </location>
        <ligand>
            <name>L-tryptophan</name>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="293"/>
            <end position="294"/>
        </location>
        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="442"/>
            <end position="444"/>
        </location>
        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>chorismate</name>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="474" mass="54300" checksum="7094E20F7C2A5124" modified="1996-11-01" version="1">MIIKKIKMDVCPLDVYEQIRGENTFLLESAEGVPKVARYSILGKAEGKVIFKNGKLKVESFTEFGDKAKDLEGKYECPLDALREVRNEYLKYIDISNIEPIPRFKGGLVGYLSYDIIRYWIDLSNINPKPINDLKFPDAEFFIVKDFISFDLKEKVINLIAEDDEGIRELERIIKNAKIGNNDNKEEKTTENKDLKIKSNMSKEEFIEAVKKAKEYIFAGDIFQVVLSRRIEIDLDNLDHLKIYKKVREINPSPYMYYLDFGDRKIIGSSPEILVRTDYKDNKRLVITRPIAGTIRRGKTEEEDKELEKKLLSDEKERAEHVMLVDLARNDIGKISKFGTVEVTDFMIIEKYSHVQHIVSNVVGELKDNYDSFLAVKATFPAGTLSGAPKVRAMEIIEELEKTWRGPYGGGVGYFGWDDLMDLAITIRTFVISKNKGYIQVGAGIVADSIPENEWEETERKGMANVKTIESLLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58495</accession>
    <name>TIAS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="1">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="1">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tiaS</name>
        <name type="ordered locus">MJ1095</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>agmatine + ATP + cytidine(34) in tRNA(Ile2) + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TiaS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64436">
        <property type="entry name" value="F64436"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58495"/>
    <dbReference type="SMR" id="Q58495"/>
    <dbReference type="STRING" id="243232.MJ_1095"/>
    <dbReference type="EnsemblBacteria" id="AAB99098">
        <property type="protein sequence ID" value="AAB99098"/>
        <property type="gene ID" value="MJ_1095"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1095"/>
    <dbReference type="eggNOG" id="arCOG01115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_675459_0_0_2"/>
    <dbReference type="InParanoid" id="Q58495"/>
    <dbReference type="OMA" id="GMCTTYL"/>
    <dbReference type="PhylomeDB" id="Q58495"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="DUF1743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="DUF1743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000107166" description="tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="OB" evidence="1">
        <location>
            <begin position="273"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <sequence length="423" mass="48557" checksum="2B8936E352DB74BB" modified="1996-11-01" version="1">MVMMFIGIDDTDSPNKYCTTYIATLLIEELKGCGYSVDMPKLIRMNPMVKYKTRGNGGVAIHILDELYSKDKEEIKNITISLVEKYTDFECENTNPGIVFLDEAKYKENREKLTNYYKKVLYDIVSVDYAEKFILKVGGEFIKYKLGRGIIGALGAISSTPPYTYELLAYRKKEMWGKKREIDEKSVIEMDKETFPYTFDNYDYENEKILIAPNTPCPVLFGIRGIDAEILLKAMHKIEGEKPERFMIFKTNHGTDVHLRKMNIKDIYPNTGVIVYGRVVEEPRDIEGGHVIFKLSDGTGEIDCMAYEPTKGFRDIIRKLIVGDYIAVYGTVREKPLGINIEKIKILKLEKKFVKDKRCPYCGGTLKAKGKKAGYKCKKCKKTIAYDEIKMIEVERDLKTGFYEVPGSARRHLSKPIQLIDLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58526</accession>
    <name>HFLX_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hflX</name>
        <name type="ordered locus">MJ1126</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64440">
        <property type="entry name" value="E64440"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58526"/>
    <dbReference type="SMR" id="Q58526"/>
    <dbReference type="STRING" id="243232.MJ_1126"/>
    <dbReference type="EnsemblBacteria" id="AAB99128">
        <property type="protein sequence ID" value="AAB99128"/>
        <property type="gene ID" value="MJ_1126"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1126"/>
    <dbReference type="eggNOG" id="arCOG00353">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019597_2_0_2"/>
    <dbReference type="InParanoid" id="Q58526"/>
    <dbReference type="OMA" id="AEMKTGR"/>
    <dbReference type="PhylomeDB" id="Q58526"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="PTHR10229"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000205444" description="GTPase HflX">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Hflx-type G" evidence="1">
        <location>
            <begin position="181"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="187"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="212"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="233"/>
            <end position="236"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="300"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="328"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <sequence length="402" mass="46482" checksum="4DA94B9D200B77AF" modified="1996-11-01" version="1">MLILRKDSKFDRKSIEELKELAEVLYNPVKTIVQIRKADPKYQIGSGLVERIAENIKEENIEIVIVGNILTPSQKYNLAKKFKVEVIDKIELVLRIFYKHARTKEAQLQVRLAELQYELPRAREKVRLAKMGEQPGFGGYGDYEVEKYYQKVKREIATIKRKLEKLREHRRVARKGRAKFDTVGLIGYTNAGKTSLLNALTGENKESKNQVFTTLTTTTRAIKGIKRKILVTDTVGFIDDLPPFMIEAFLSTIEESADSDLILIVVDASDDIEEIKRKLKVNHEILSKINCKAPIITVFNKVDKITKEKKRKILEELDRYIVNPIFVSAKYDINMDLLKEMIIEHLNLSIGTIETDNPRLISYLYENTEIIEDILEDNKHIITFRAKERDVNRILKLHKSAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58527</accession>
    <name>Y1127_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1127</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1127</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64440">
        <property type="entry name" value="F64440"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58527"/>
    <dbReference type="SMR" id="Q58527"/>
    <dbReference type="STRING" id="243232.MJ_1127"/>
    <dbReference type="DNASU" id="1452023"/>
    <dbReference type="EnsemblBacteria" id="AAB99129">
        <property type="protein sequence ID" value="AAB99129"/>
        <property type="gene ID" value="MJ_1127"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1127"/>
    <dbReference type="eggNOG" id="arCOG00893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_985571_0_0_2"/>
    <dbReference type="InParanoid" id="Q58527"/>
    <dbReference type="OMA" id="CAVQLHT"/>
    <dbReference type="PhylomeDB" id="Q58527"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011589">
        <property type="entry name" value="UCP004961"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206">
        <property type="entry name" value="PTHR42206"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004961">
        <property type="entry name" value="UCP004961_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107180" description="Uncharacterized protein MJ1127">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="280" mass="31672" checksum="6FC7CD2F0E85C959" modified="1996-11-01" version="1">MDVLKSLPVTDNHIHVDDKHGYGAEKVAKTFYNAGGKVMIVLNKPTFDGNLTASMDILVRDVEIINKNTPVKAFGLVGVHPAELTYLMKFMSLEEAKQRIVDALNYAKKLVEEYDFIVGIGEVGRPHYPVSEDVWKASNEILKYCMELAKDIGCAIQIHAESSTEEQFKEFSEMAKEVGLNPEKVVKHHCGNMVLEGERYGIFPSILASRVNEDVVKKSLRFVMETDYIDDLKRPGVALGIKTVPRVTRRLIEKGVLDEEGVYKIHKENIERIYDMDLEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58553</accession>
    <name>Y1153_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1153</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1153</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64443">
        <property type="entry name" value="H64443"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58553"/>
    <dbReference type="SMR" id="Q58553"/>
    <dbReference type="STRING" id="243232.MJ_1153"/>
    <dbReference type="EnsemblBacteria" id="AAB99159">
        <property type="protein sequence ID" value="AAB99159"/>
        <property type="gene ID" value="MJ_1153"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1153"/>
    <dbReference type="HOGENOM" id="CLU_2893257_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107191" description="Uncharacterized protein MJ1153">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <sequence length="62" mass="7619" checksum="BE817CC65B3D3063" modified="1996-11-01" version="1">MKENILKQKWAKIMKKHKYKKPKWLIKAEQKAEKMTLEEEKEWIEKAIRVYKDKGIDGLFEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58555</accession>
    <name>Y1155_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0313 protein MJ1155</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1155</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0313 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64444">
        <property type="entry name" value="B64444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870666.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58555"/>
    <dbReference type="STRING" id="243232.MJ_1155"/>
    <dbReference type="PRIDE" id="Q58555"/>
    <dbReference type="EnsemblBacteria" id="AAB99152">
        <property type="protein sequence ID" value="AAB99152"/>
        <property type="gene ID" value="MJ_1155"/>
    </dbReference>
    <dbReference type="GeneID" id="1452051"/>
    <dbReference type="KEGG" id="mja:MJ_1155"/>
    <dbReference type="eggNOG" id="arCOG04984">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018288_2_0_2"/>
    <dbReference type="InParanoid" id="Q58555"/>
    <dbReference type="OMA" id="TCMYYTG"/>
    <dbReference type="OrthoDB" id="8457at2157"/>
    <dbReference type="PhylomeDB" id="Q58555"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01251">
        <property type="entry name" value="UPF0313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022946">
        <property type="entry name" value="UPF0313"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024560">
        <property type="entry name" value="UPF0313_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013704">
        <property type="entry name" value="UPF0313_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32331">
        <property type="entry name" value="PTHR32331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11842">
        <property type="entry name" value="DUF3362"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08497">
        <property type="entry name" value="Radical_SAM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01069">
        <property type="entry name" value="UPF0313"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03904">
        <property type="entry name" value="SAM_YgiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000076404" description="UPF0313 protein MJ1155">
        <location>
            <begin position="1"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="285"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="603" mass="70425" checksum="B3AB76483FEE6444" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58557</accession>
    <name>Y1158_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1158</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1158</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64444">
        <property type="entry name" value="D64444"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58557"/>
    <dbReference type="STRING" id="243232.MJ_1158"/>
    <dbReference type="EnsemblBacteria" id="AAB99174">
        <property type="protein sequence ID" value="AAB99174"/>
        <property type="gene ID" value="MJ_1158"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1158"/>
    <dbReference type="eggNOG" id="arCOG05070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157007_0_0_2"/>
    <dbReference type="OMA" id="RNCGFTR"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107196" description="Uncharacterized protein MJ1158">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <sequence length="129" mass="15359" checksum="FFD2D590CCC5C7B0" modified="1996-11-01" version="1">MWGIQHFGDFMEKWELKKLAVCFNCKKEADQIIEIYTNQAFVKCSNCGATRYYILRRVGIEDESIIEDEKNKKHKYEPWFLEKTAVCFNCKKEATQDIAITETKMIVRCRNCGFTRVYQFHILDIPENK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58558</accession>
    <name>Y1157_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable sulfurtransferase</fullName>
            <ecNumber evidence="2">2.8.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1157</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TtcA family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64444">
        <property type="entry name" value="E64444"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58558"/>
    <dbReference type="SMR" id="Q58558"/>
    <dbReference type="STRING" id="243232.MJ_1157"/>
    <dbReference type="DNASU" id="1452056"/>
    <dbReference type="EnsemblBacteria" id="AAB99161">
        <property type="protein sequence ID" value="AAB99161"/>
        <property type="gene ID" value="MJ_1157"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1157"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_2_2"/>
    <dbReference type="InParanoid" id="Q58558"/>
    <dbReference type="OMA" id="MNLDQKQ"/>
    <dbReference type="PhylomeDB" id="Q58558"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020554">
        <property type="entry name" value="UPF0021_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00269">
        <property type="entry name" value="TIGR00269"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01263">
        <property type="entry name" value="UPF0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000219891" description="Probable sulfurtransferase">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O58038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="341" mass="40094" checksum="B87D2AC28D6C18C3" modified="1996-11-01" version="1">MLDSPHNLYIKKFMLCYIIYYFLICHLYNHIFNLREIMLCSCGNEAFYYQKYSNRHLCKECFKKDIERRAKKVLGKDIIRNNVKIGIGISGGKDSLVMAYILKELFKHIPNAKLICFFVDEGIKGFRNIAEKYVKEFCKEYNLDLKIIKFEDEIGYTLDEIVKNDYLSKLNIGKPCSFCGVVRRYLLNKHALKEGCDYLAIGHNLDDFCQTILMNYVEGNIKNIIQFGKEFEGGGFVKRIKPLKLIPEEEVKLYAEINNIKYQREPCPYSSLSYRHRMKKVIEILEEEKPGVKFSILRGYEKLLKYLNVKEEIRRCEICGFPCSGNICKVCSWLIKLKEIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58575</accession>
    <name>AROC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Chorismate synthase</fullName>
            <shortName evidence="1">CS</shortName>
            <ecNumber evidence="1">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroC</name>
        <name type="ordered locus">MJ1175</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="1">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the chorismate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64446">
        <property type="entry name" value="F64446"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58575"/>
    <dbReference type="SMR" id="Q58575"/>
    <dbReference type="STRING" id="243232.MJ_1175"/>
    <dbReference type="EnsemblBacteria" id="AAB99178">
        <property type="protein sequence ID" value="AAB99178"/>
        <property type="gene ID" value="MJ_1175"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1175"/>
    <dbReference type="eggNOG" id="arCOG04133">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034547_0_2_2"/>
    <dbReference type="InParanoid" id="Q58575"/>
    <dbReference type="OMA" id="MLSINAV"/>
    <dbReference type="PhylomeDB" id="Q58575"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="PTHR21085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="SSF103263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00789">
        <property type="entry name" value="CHORISMATE_SYNTHASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140690" description="Chorismate synthase">
        <location>
            <begin position="1"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
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            <position position="50"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="127"/>
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        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="255"/>
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        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="315"/>
            <end position="319"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <sequence length="378" mass="41615" checksum="DEA200AFC32B0CF5" modified="1996-11-01" version="1">MVTLMNTYGDMFRVTVFGESHGKAVGAVVDGCPANLPLSEEDIQKELDRRRPGQSIFSTPRKEEDKVEILSGIFEGKTTGAPICSIVYNKNMRPKDYSKIKDTPRPGHADLTYRLKYKNYDYRGGGRASGRVTIGHVIGGAIAKKLLSYTYNIKIIGYTIKIGKIEGDFSYYKNPEVFENEKSLERLIEIIESNPLRCPSMNEKEMEEYVLKAMENKDSVGGVVEIVALNVPVGVGNPIFNKLNGELARALMSINAVKGVEIGAGFKAAEMYGSEMNDEMYFDDDKNIRFKTNNCGGILGGISCGTPIVLRIAVKPTPSIGKKQKTINLKTLENVEIEIEGRHDPVIVPRIVPVAEAMVAITLADLMIKGGFIHPCSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58586</accession>
    <name>WTPA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Molybdate/tungstate-binding protein WtpA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">wtpA</name>
        <name type="ordered locus">MJ1186</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Binds tungstate and molybdate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64448">
        <property type="entry name" value="A64448"/>
    </dbReference>
    <dbReference type="PDB" id="3CFZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=37-325"/>
    </dbReference>
    <dbReference type="PDBsum" id="3CFZ"/>
    <dbReference type="AlphaFoldDB" id="Q58586"/>
    <dbReference type="SMR" id="Q58586"/>
    <dbReference type="STRING" id="243232.MJ_1186"/>
    <dbReference type="TCDB" id="3.A.1.6.9">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99190">
        <property type="protein sequence ID" value="AAB99190"/>
        <property type="gene ID" value="MJ_1186"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1186"/>
    <dbReference type="eggNOG" id="arCOG00219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055936_0_0_2"/>
    <dbReference type="InParanoid" id="Q58586"/>
    <dbReference type="OMA" id="GYRTVMM"/>
    <dbReference type="PhylomeDB" id="Q58586"/>
    <dbReference type="EvolutionaryTrace" id="Q58586"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901359">
        <property type="term" value="F:tungstate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022498">
        <property type="entry name" value="ABC_trnspt_W-bd_WtpA"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03730">
        <property type="entry name" value="tungstate_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0500">Molybdenum</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000159720" description="Molybdate/tungstate-binding protein WtpA">
        <location>
            <begin position="20"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="46"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="46"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>tungstate</name>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>molybdate</name>
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        </ligand>
    </feature>
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        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>tungstate</name>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="158"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>molybdate</name>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="158"/>
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        <ligand>
            <name>tungstate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>molybdate</name>
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        </ligand>
    </feature>
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        </location>
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            <name>tungstate</name>
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        </ligand>
    </feature>
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            <position position="239"/>
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        <ligand>
            <name>molybdate</name>
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        </ligand>
    </feature>
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        <location>
            <position position="239"/>
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        <ligand>
            <name>tungstate</name>
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        </ligand>
    </feature>
    <feature type="strand" evidence="5">
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    <feature type="helix" evidence="5">
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    </feature>
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    </feature>
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        <location>
            <begin position="288"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="300"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
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            <begin position="315"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="319"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O30142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3CFZ"/>
        </source>
    </evidence>
    <sequence length="346" mass="39445" checksum="85009C2115769C40" modified="1996-11-01" version="1" precursor="true">MIKRLIVISILLIVGTVLCGCMEQENVGQQNSEAQEKIVLKIFHAGSLSVPFEEYEKMFEKEHPNVDVEREPAGSVACVRKIIDLGKKADILASADYSLIPQMMMPKYADWYVMFARNEIVLAYTDKSKYKDEINSTNWYKILQRPDVKIGFSNPNDDPCGYRTQMVLQLAELYYKDPTIYDNLVLKHSNIKVEENNGTYLILVPKELDVDTNKLFVRSKETDLLAPLEAGAFDYLFIYKSVANQHHLKYIELPKEINLGYYEYADTYKKVALKIIAKNKTINAKPIVYGMTVPTNAPHKKEAIEFVKFVLGHPEVLENNGQPAIIPAVAYGNVPEELKDLVKIEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q58656</accession>
    <name>PYRH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Uridylate kinase</fullName>
            <shortName evidence="1">UK</shortName>
            <ecNumber evidence="1">2.7.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Uridine monophosphate kinase</fullName>
            <shortName evidence="1">UMP kinase</shortName>
            <shortName evidence="1">UMPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrH</name>
        <name type="ordered locus">MJ1259</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible phosphorylation of UMP to UDP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + UMP = ADP + UDP</text>
            <dbReference type="Rhea" id="RHEA:24400"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.22"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by UTP.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UMP kinase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99262" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.4.22" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99262.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64457">
        <property type="entry name" value="B64457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496770.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58656"/>
    <dbReference type="SMR" id="Q58656"/>
    <dbReference type="STRING" id="243232.MJ_1259"/>
    <dbReference type="DNASU" id="1452157"/>
    <dbReference type="EnsemblBacteria" id="AAB99262">
        <property type="protein sequence ID" value="AAB99262"/>
        <property type="gene ID" value="MJ_1259"/>
    </dbReference>
    <dbReference type="GeneID" id="1452157"/>
    <dbReference type="KEGG" id="mja:MJ_1259"/>
    <dbReference type="eggNOG" id="arCOG00858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079546_0_0_2"/>
    <dbReference type="InParanoid" id="Q58656"/>
    <dbReference type="OMA" id="MGGTHPG"/>
    <dbReference type="OrthoDB" id="48934at2157"/>
    <dbReference type="PhylomeDB" id="Q58656"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00275"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033862">
        <property type="term" value="F:UMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006225">
        <property type="term" value="P:UDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04253">
        <property type="entry name" value="AAK_UMPK-PyrH-Pf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01220_A">
        <property type="entry name" value="PyrH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001057">
        <property type="entry name" value="Glu/AcGlu_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011817">
        <property type="entry name" value="Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011818">
        <property type="entry name" value="Uridylate_kinase_arch/spir"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005650">
        <property type="entry name" value="Uridylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00474">
        <property type="entry name" value="GLU5KINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02076">
        <property type="entry name" value="pyrH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000143917" description="Uridylate kinase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="116"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>UMP</name>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="226" mass="24099" checksum="7052087EE2AE240D" modified="2002-08-30" version="2">MRIVFDLGGSVVMPKEGAKAEKIMEYANIFKKIKDEGHEVAIVVGGGKTAREYIEIGRELGASESFCDELGIMATRMNAMILITALGDYSIKKVPTSFEEAELILNLGKIPVMGGTHPGHTTDAVAASLAEFINADLLVIGTNVDGVYDKDPNKYEDAKKFDKMSAKELVDLAISSSLKAGSSSVVDLLAAKIIERAKLKVAVVKGTPEELLNVSKGIINGTIIEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="145" xmlns="http://uniprot.org/uniprot">
    <accession>Q58657</accession>
    <name>IF2G_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">eif2g</name>
        <name type="ordered locus">MJ1261</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="10634" last="10642">
            <title>X-ray structure of translation initiation factor eIF2gamma: implications for tRNA and eIF2alpha binding.</title>
            <authorList>
                <person name="Roll-Mecak A."/>
                <person name="Alone P."/>
                <person name="Cao C."/>
                <person name="Dever T.E."/>
                <person name="Burley S.K."/>
            </authorList>
            <dbReference type="PubMed" id="14688270"/>
            <dbReference type="DOI" id="10.1074/jbc.M310418200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 9-411 IN COMPLEX WITH ZINC</scope>
    </reference>
    <comment type="function">
        <text evidence="2">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>GTP + H2O = GDP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64457">
        <property type="entry name" value="D64457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496772.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1S0U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=9-411"/>
    </dbReference>
    <dbReference type="PDBsum" id="1S0U"/>
    <dbReference type="AlphaFoldDB" id="Q58657"/>
    <dbReference type="SMR" id="Q58657"/>
    <dbReference type="STRING" id="243232.MJ_1261"/>
    <dbReference type="EnsemblBacteria" id="AAB99264">
        <property type="protein sequence ID" value="AAB99264"/>
        <property type="gene ID" value="MJ_1261"/>
    </dbReference>
    <dbReference type="GeneID" id="1452159"/>
    <dbReference type="KEGG" id="mja:MJ_1261"/>
    <dbReference type="eggNOG" id="arCOG01563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027154_0_1_2"/>
    <dbReference type="InParanoid" id="Q58657"/>
    <dbReference type="OMA" id="NIGMVGH"/>
    <dbReference type="OrthoDB" id="25592at2157"/>
    <dbReference type="PhylomeDB" id="Q58657"/>
    <dbReference type="EvolutionaryTrace" id="Q58657"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00119">
        <property type="entry name" value="eIF_2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
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<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
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    <name>Y1303_METJA</name>
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            <fullName>Uncharacterized polyferredoxin-like protein MJ1303</fullName>
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    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
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            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58713</accession>
    <name>Y1317_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized MFS-type transporter MJ1317</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1317</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB99324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D64464"/>
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    <dbReference type="AlphaFoldDB" id="Q58713"/>
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        <property type="gene ID" value="MJ_1317"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1317"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_040020_1_0_2"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000084886" description="Uncharacterized MFS-type transporter MJ1317">
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            <begin position="1"/>
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        </location>
    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="398" mass="44097" checksum="99CBE61675FD7D06" modified="1996-11-01" version="1">MVRKVAEQNLQKNNENDRELSKNVYLLGFTSFLNDMSSEMIMPILPMLITSVGGGSLSIGLVGGLREFISNILMVLIGYCSDKVRKRKIFVVLGYLTSSMFKLLLGLSKSWLGAVIFSSLERMGKGIRTAPRDAIISESMPKTLGKGFGIQRAFDTAGAILGSTLSLLFILYLQYSFNQIILIAAVIGFLTLIPLYFVKEKPSPSNNKITFRVGIKNLPKELKLFILISAIFTLSNFSYMFYILRAQEFLMIVDEKMAIIIPIALYILYNIFYATFSIPFGILSDKIGRKSVLTIGYIVYGIVSLGFAYFISQKSLILLFALYGIAYALFAGNQKAYVSDLSSEDIRATALGLFYTVVGLTSLPASLIAGYLWKISPEMTFLYGSVLAIISGLLLLFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58734</accession>
    <name>HMDY_METJA</name>
    <protein>
        <recommendedName>
            <fullName>H(2)-forming methylenetetrahydromethanopterin dehydrogenase-related protein MJ1338</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1338</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMD family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="hmd_rel"/>
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    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000218518" description="H(2)-forming methylenetetrahydromethanopterin dehydrogenase-related protein MJ1338">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="4"/>
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        </location>
    </feature>
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        </location>
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    <feature type="helix" evidence="2">
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        </location>
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        </location>
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        </location>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="turn" evidence="2">
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
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        </location>
    </feature>
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        </location>
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    <feature type="strand" evidence="2">
        <location>
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        </location>
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        <location>
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    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="273"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="290"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="304"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="323"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="4YT2"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="6HUX"/>
        </source>
    </evidence>
    <sequence length="353" mass="38212" checksum="2AFF9FD87675F6A6" modified="1996-11-01" version="1">MRNIRKIKVDNMKVSVYGAGNQNLYINKLNLPEKFGGEPPYGGSRMAIEFAEAGHDVVLAEPNKNIMSDDLWKKVEDAGVKVVSDDVEAAKHGEIHVLFTPFGKATFRIAKTIIEHVPENAVICNTCTVSPVVLYYSLEPILRTKRKDVGISSMHPAAVPGTPQHGHYVIGGKTTDGKELATEEQIKKAVELAKSAGKEAYVVPADVSSVVADMGSLVTAVALSGVLDYYTVGRKIINAPKKMIEQQVIMTLQTMASLVETSGIEGMVKALNPELLIRSASSMKLLDRQKDLDAALEILQNLDETLKAEVEKAEIKPTTLVAAQSLVKEIKTLIGGAAAEGAIKRSARKLFEH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58786</accession>
    <name>DAPAT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">LL-diaminopimelate aminotransferase</fullName>
            <shortName evidence="3">DAP-AT</shortName>
            <shortName evidence="3">DAP-aminotransferase</shortName>
            <shortName evidence="3">LL-DAP-aminotransferase</shortName>
            <ecNumber evidence="2">2.6.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">dapL</name>
        <name type="ordered locus">MJ1391</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="J. Bacteriol." volume="192" first="3304" last="3310">
            <title>Methanococci use the diaminopimelate aminotransferase (DapL) pathway for lysine biosynthesis.</title>
            <authorList>
                <person name="Liu Y."/>
                <person name="White R.H."/>
                <person name="Whitman W.B."/>
            </authorList>
            <dbReference type="PubMed" id="20418392"/>
            <dbReference type="DOI" id="10.1128/JB.00172-10"/>
        </citation>
        <scope>FUNCTION AS A DIAMINOPIMELATE AMINOTRANSFERASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2S,6S)-2,6-diaminoheptanedioate + 2-oxoglutarate = (S)-2,3,4,5-tetrahydrodipicolinate + H(+) + H2O + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23988"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="EC" id="2.6.1.83"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.42 uM for alpha-ketoglutarate (alpha-KG)(at pH 8.8 and at 70 degrees Celsius)</KM>
            <KM evidence="2">82.8 uM for LL-2,6-diaminopimelate (L,L-DAP)(at pH 8.8 and at 70 degrees Celsius)</KM>
            <Vmax evidence="2">0.39 umol/min/mg enzyme (at pH 8.8 and at 70 degrees Celsius)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 8.5. About 40% and 50% of the maximum activity are observed at pH 7.0 and 10.0, respectively.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 70 degrees Celsius. No activity is detected at temperatures below 45 degrees Celsius. At 85 degrees Celsius, the activity is 85% of the maximum activity.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (aminotransferase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
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    <dbReference type="EnsemblBacteria" id="AAB99401">
        <property type="protein sequence ID" value="AAB99401"/>
        <property type="gene ID" value="MJ_1391"/>
    </dbReference>
    <dbReference type="GeneID" id="1452294"/>
    <dbReference type="KEGG" id="mja:MJ_1391"/>
    <dbReference type="eggNOG" id="arCOG01133">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_5_2"/>
    <dbReference type="InParanoid" id="Q58786"/>
    <dbReference type="OMA" id="YPHMPTG"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
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    <dbReference type="BioCyc" id="MetaCyc:MON-15639"/>
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        <property type="reaction ID" value="UER00466"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010285">
        <property type="term" value="F:L,L-diaminopimelate aminotransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033362">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate, diaminopimelate-aminotransferase pathway"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
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    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123929" description="LL-diaminopimelate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="248"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O84395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="20418392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="20418392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="418" mass="47709" checksum="73CFBC48B4CA23F6" modified="1996-11-01" version="1">MESYIQNLFAERIGGKKFGKEDVIYKFEKIKRAKQEAMKRHPDMELIDMGVGEPDEMADPEVIRVLCEEAKKWENRGYADNGIQELKDAVPPYMEKVYGVKDIDPVNEVIHSIGSKPALAYITSAFINPGDVCLMTVPGYPVTATHTKWYGGEVYNLPLLEENDFLPDLESIPEDIKKRAKILYLNYPNNPTGAQATKKFYKEVVDFAFENEVIVVQDAAYGALVYDGKPLSFLSVKDAKEVGVEIHSFSKAFNMTGWRLAFLVGNELIIKAFATVKDNFDSGQFIPIQKAGIYCLQHPEITERVRQKYERRLRKMVKILNEVGFKARMPGGTFYLYVKSPTKANGIEFKTAEDFSQYLIKEKLISTVPWDDAGHYLRLAACFVAKDENGNPTTEEKYEDMVLEEFKRRLEGMDLEFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58833</accession>
    <name>COBS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="1">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cobS</name>
        <name type="ordered locus">MJ1438</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosylcob(III)inamide-GDP + alpha-ribazole 5'-phosphate = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64479">
        <property type="entry name" value="E64479"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58833"/>
    <dbReference type="STRING" id="243232.MJ_1438"/>
    <dbReference type="EnsemblBacteria" id="AAB99447">
        <property type="protein sequence ID" value="AAB99447"/>
        <property type="gene ID" value="MJ_1438"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1438"/>
    <dbReference type="eggNOG" id="arCOG04338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057426_2_0_2"/>
    <dbReference type="InParanoid" id="Q58833"/>
    <dbReference type="OMA" id="CACCGIP"/>
    <dbReference type="PhylomeDB" id="Q58833"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="PTHR34148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000146910" description="Adenosylcobinamide-GDP ribazoletransferase">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <sequence length="258" mass="28778" checksum="3C395D39A5DA1169" modified="1996-11-01" version="1">MGITMFKEFKALLSFFTRIPIYVEDFDFENIANYFYLIILIGYVFGIFSLILGYIFSFLLPNFLSAVLILFFIEYLNGFHHIDGLIDFGDGWMAVGDKRKKLMAMKDRYIGCGGVVFAIFFNLMAVISLSYILDINILYLLVGEVCAKLGMLSCSTFGNPLIEGTGRYFVKKADEKFLTIGIILSLPLLLIFSGIERKIVIIAIITTIITGLCMAKIAKRHFGGVNGDVLGASNEITRVVVLLSIIASIKVFSIYLLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58834</accession>
    <name>Y1439_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1439</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1439</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the thermonuclease family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64479">
        <property type="entry name" value="F64479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870957.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58834"/>
    <dbReference type="SMR" id="Q58834"/>
    <dbReference type="STRING" id="243232.MJ_1439"/>
    <dbReference type="EnsemblBacteria" id="AAB99448">
        <property type="protein sequence ID" value="AAB99448"/>
        <property type="gene ID" value="MJ_1439"/>
    </dbReference>
    <dbReference type="GeneID" id="1452343"/>
    <dbReference type="KEGG" id="mja:MJ_1439"/>
    <dbReference type="eggNOG" id="arCOG03192">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046484_5_3_2"/>
    <dbReference type="InParanoid" id="Q58834"/>
    <dbReference type="OMA" id="DTPETYQ"/>
    <dbReference type="OrthoDB" id="92842at2157"/>
    <dbReference type="PhylomeDB" id="Q58834"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002071">
        <property type="entry name" value="Thermonucl_AS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="SSF50199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01123">
        <property type="entry name" value="TNASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01284">
        <property type="entry name" value="TNASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000215283" description="Uncharacterized protein MJ1439">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00272"/>
        </source>
    </evidence>
    <sequence length="207" mass="24547" checksum="4866BC9AAB498906" modified="1996-11-01" version="1">MIRGRIMRKFLILSMLIFTTLCGCVDFSSSNSNGYYHDSWSYNNYTSFVDTHEHYYGKVVKVVDGDTVYVEVNGELWKIRLLGVDTPEIHKRNNPYEYYLLNGTPITDTKYLKEWGYKAKHFAEKELKNKTVIIVFDNEAPKKDKYGRYLAYIFINNSNNLINFNEELLKYGYARVYISNFELKDEFLNVEREAKENRVGLWNWSNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q58857</accession>
    <name>Y1462_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1462</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1462</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64482">
        <property type="entry name" value="E64482"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58857"/>
    <dbReference type="SMR" id="Q58857"/>
    <dbReference type="STRING" id="243232.MJ_1462"/>
    <dbReference type="EnsemblBacteria" id="AAB99476">
        <property type="protein sequence ID" value="AAB99476"/>
        <property type="gene ID" value="MJ_1462"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1462"/>
    <dbReference type="eggNOG" id="arCOG08274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2565887_0_0_2"/>
    <dbReference type="OMA" id="HATKYED"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107351" description="Uncharacterized protein MJ1462">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <sequence length="83" mass="9511" checksum="F1BD6B842BA834CD" modified="1996-11-01" version="1">MMIYGILLNIPEKHATKYEDLIRRIIGEGIARGDILSFTEARYKGDVAFVMLARSRRAAEKVYQQLKEHPIHVKVIEIEGKGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58866</accession>
    <name>Y1471_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1471</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1471</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64483">
        <property type="entry name" value="F64483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870992.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58866"/>
    <dbReference type="STRING" id="243232.MJ_1471"/>
    <dbReference type="PRIDE" id="Q58866"/>
    <dbReference type="EnsemblBacteria" id="AAB99486">
        <property type="protein sequence ID" value="AAB99486"/>
        <property type="gene ID" value="MJ_1471"/>
    </dbReference>
    <dbReference type="GeneID" id="1452376"/>
    <dbReference type="KEGG" id="mja:MJ_1471"/>
    <dbReference type="eggNOG" id="arCOG05085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1514623_0_0_2"/>
    <dbReference type="OMA" id="NRSEGWY"/>
    <dbReference type="OrthoDB" id="89695at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107357" description="Uncharacterized protein MJ1471">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="178" mass="20692" checksum="23B5B6625792B4EE" modified="1996-11-01" version="1">MINSVDLAIGTAILLIGMAYWTVSIVEHNNNYVDIVKSDYIFDKGISTMEHLSEDGTLQDAVLLYYFDRVNDSKKLLEERIPLKHYLLYIDNNLLINKSNGVNNSNSVYILTVLTLNRSEGWYVIYGNEDFVNISKERFLDYDDAYNYLKYRNYDIHMPVYLSKNVSSSRVELYILGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58867</accession>
    <name>Y1472_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1472</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1472</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64483">
        <property type="entry name" value="G64483"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870993.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58867"/>
    <dbReference type="STRING" id="243232.MJ_1472"/>
    <dbReference type="EnsemblBacteria" id="AAB99487">
        <property type="protein sequence ID" value="AAB99487"/>
        <property type="gene ID" value="MJ_1472"/>
    </dbReference>
    <dbReference type="GeneID" id="1452377"/>
    <dbReference type="KEGG" id="mja:MJ_1472"/>
    <dbReference type="eggNOG" id="arCOG03508">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041883_0_0_2"/>
    <dbReference type="OMA" id="LPYWVQT"/>
    <dbReference type="OrthoDB" id="40364at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018765">
        <property type="entry name" value="DUF2341"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10102">
        <property type="entry name" value="DUF2341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014016" description="Uncharacterized protein MJ1472">
        <location>
            <begin position="23"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="544" mass="63400" checksum="A3E93AD51C94EBF0" modified="1998-06-01" version="2" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58883</accession>
    <name>Y1488_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1488</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1488</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64485">
        <property type="entry name" value="G64485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871010.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58883"/>
    <dbReference type="SMR" id="Q58883"/>
    <dbReference type="STRING" id="243232.MJ_1488"/>
    <dbReference type="DNASU" id="1452394"/>
    <dbReference type="EnsemblBacteria" id="AAB99500">
        <property type="protein sequence ID" value="AAB99500"/>
        <property type="gene ID" value="MJ_1488"/>
    </dbReference>
    <dbReference type="GeneID" id="1452394"/>
    <dbReference type="KEGG" id="mja:MJ_1488"/>
    <dbReference type="eggNOG" id="arCOG01521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_668371_0_0_2"/>
    <dbReference type="InParanoid" id="Q58883"/>
    <dbReference type="OMA" id="ALHMIPH"/>
    <dbReference type="OrthoDB" id="20763at2157"/>
    <dbReference type="PhylomeDB" id="Q58883"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107372" description="Uncharacterized protein MJ1488">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <sequence length="393" mass="43396" checksum="AEE623668282B340" modified="1996-11-01" version="1">MRIGIVGAGLGGLLAGALLSKNHEVVVFEKLPFLGGRFTNLKYEGFQLTTGALHMIPHGNDGYLAQALRKAGANVKIINSKPDGTFLINGKEYLYKELFSLLGFKEKAKAFKLATKLKLGKVDKNISFGEFLEEIDLALKVGNAFTGWALSLTAYETPMSEIIEIAKNYHKFGGPGIPIGGCKAVTDELSRIIKKNNGKIIKEYEVKRIEIDEKAYIDDYEFDVVISNISPIETQKICNIKFLKSKPKPSKGIKISIATKEGIIKHGGVLFTPECERINGLNQVTNVDKSLAPEGWHLVMTHQTQLTNNVKKEIDLGLEDIENLFKGKDYKILHIQSYRDDWPVNHASNGTDIDNIVNDRFYLVGDGAKGRGGIEVEGIAMGVLKVVNYINSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58888</accession>
    <name>Y1493_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1493</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1493</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64486">
        <property type="entry name" value="D64486"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58888"/>
    <dbReference type="STRING" id="243232.MJ_1493"/>
    <dbReference type="EnsemblBacteria" id="AAB99511">
        <property type="protein sequence ID" value="AAB99511"/>
        <property type="gene ID" value="MJ_1493"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1493"/>
    <dbReference type="eggNOG" id="arCOG03430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1207638_0_0_2"/>
    <dbReference type="OMA" id="CKHAYAL"/>
    <dbReference type="PhylomeDB" id="Q58888"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000107377" description="Uncharacterized protein MJ1493">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="SWIM-type" evidence="1">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <sequence length="231" mass="27883" checksum="173F6F3D7F1402AC" modified="1996-11-01" version="1">MITMNYDPKIIERGKLYYRNNLVKYCIKYKNFLFGEVVGSDTYKVKVDLDNNYFGLCTCQYKYNCKHAYALIEAYENNNYVDAEEIFKEIEDKPKEEILKILKNLVVKYYLWDEFLNTDSLLNKAIGLIKLIPLERKNIYTFKSFLRNQFVKNADDEELIKVIDEMIRADLDFNNSDIIEALTIILDEIFRRENKEAIKKLINLYRKHKKELWIVGDYLIEYYDNYFDYED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58894</accession>
    <name>C2M5_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative control protein C.MjaVP</fullName>
            <shortName evidence="1">C.MjaVP</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1499</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in control of expression of the type II restriction enzyme MjaV and/or its methyltransferase M.MjaV.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64487">
        <property type="entry name" value="B64487"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871022.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58894"/>
    <dbReference type="SMR" id="Q58894"/>
    <dbReference type="STRING" id="243232.MJ_1499"/>
    <dbReference type="REBASE" id="3907">
        <property type="entry name" value="C.MjaVP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99512">
        <property type="protein sequence ID" value="AAB99512"/>
        <property type="gene ID" value="MJ_1499"/>
    </dbReference>
    <dbReference type="GeneID" id="1452406"/>
    <dbReference type="KEGG" id="mja:MJ_1499"/>
    <dbReference type="eggNOG" id="arCOG09627">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1648316_0_0_2"/>
    <dbReference type="OrthoDB" id="138957at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107379" description="Putative control protein C.MjaVP">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <sequence length="160" mass="17963" checksum="70E0B08F3E8E8C3C" modified="1996-11-01" version="1">MSKSGNKNQNCPKCNNSPWIQRANNFIAQNQNVQTGTKEYYQVEAVKYLLNNGHCGIDCRAKISDIIKGINYPKNREAFQHEVLIPLKQYGIIATLVYPGRKGGVFIPCNNDEIKKVAKQVFKRIESELENLEGSATGVQNIKNLANSLKTTVHNLKNTI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58942</accession>
    <name>Y1547_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ1547</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ1547</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1547</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable antitoxin component of a putative type VII toxin-antitoxin (TA) system. Neutralizes cognate toxic MJ1548 by di-AMPylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Probably forms a complex with cognate toxin MJ1548.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64493">
        <property type="entry name" value="B64493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871071.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58942"/>
    <dbReference type="SMR" id="Q58942"/>
    <dbReference type="STRING" id="243232.MJ_1547"/>
    <dbReference type="EnsemblBacteria" id="AAB99565">
        <property type="protein sequence ID" value="AAB99565"/>
        <property type="gene ID" value="MJ_1547"/>
    </dbReference>
    <dbReference type="GeneID" id="1452455"/>
    <dbReference type="KEGG" id="mja:MJ_1547"/>
    <dbReference type="eggNOG" id="arCOG02107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_154345_0_0_2"/>
    <dbReference type="InParanoid" id="Q58942"/>
    <dbReference type="OMA" id="SYAKNEY"/>
    <dbReference type="OrthoDB" id="115826at2157"/>
    <dbReference type="PhylomeDB" id="Q58942"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041633">
        <property type="entry name" value="Polbeta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18765">
        <property type="entry name" value="Polbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107401" description="Putative protein adenylyltransferase MJ1547">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="122" mass="14759" checksum="0992EFA439E40EF9" modified="1996-11-01" version="1">MSKVFGILLYGSYAKNEYTKRSDIDICLVGVDRNTYLEILGKLGNKYDIKIFEELPLYIKMEIIKNHKVIFGDELELSEHFYKFRKIWRDMEKRIRENQFNSVREKVMLRRRFNAKKEEILG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58944</accession>
    <name>Y1549_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1549</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1549</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64493">
        <property type="entry name" value="D64493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871073.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58944"/>
    <dbReference type="SMR" id="Q58944"/>
    <dbReference type="STRING" id="243232.MJ_1549"/>
    <dbReference type="EnsemblBacteria" id="AAB99567">
        <property type="protein sequence ID" value="AAB99567"/>
        <property type="gene ID" value="MJ_1549"/>
    </dbReference>
    <dbReference type="GeneID" id="1452457"/>
    <dbReference type="KEGG" id="mja:MJ_1549"/>
    <dbReference type="eggNOG" id="arCOG01278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035425_4_0_2"/>
    <dbReference type="InParanoid" id="Q58944"/>
    <dbReference type="OMA" id="PSLYAMM"/>
    <dbReference type="OrthoDB" id="44046at2157"/>
    <dbReference type="PhylomeDB" id="Q58944"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="SSF53901"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107402" description="Uncharacterized protein MJ1549">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <sequence length="392" mass="42148" checksum="06150891366789BA" modified="1996-11-01" version="1">MRDVAIIGYGQTKFGELWERSFRSLIVEAGVKAVEAAGIDGKDIDEMYVGNMSAGLFVGQEHIASLIAEHAGLNPIPSTRVEAACASGSLALRQAVLNVASGASDVVLVGGVEKMTDVVDATSAISSASDQEWEALFGATFPSLYAMMAQRYMYEYGLTLEELSMWSVIMHENASKNRYAQFPFKVTLEQVLNSSPVAEPLRLLHCSPVSDGAAALIVCEAEKAKEFVNKDDIIYIKASVQASDTIALHSRESITSLKAAKVASEKAYKMANIEPKDVDVAEVHDCFAINGLILMEELGFCKKGEAGKIVYDKKIAIDYDGFPAVNPSGGLKAAGHALGATGIRQVGEIYWQLKQDKEVKDRQVEIKNGYGITVNVGGTGGTVCIHILSDKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q58945</accession>
    <name>Y1550_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1550</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1550</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64493">
        <property type="entry name" value="E64493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871074.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58945"/>
    <dbReference type="STRING" id="243232.MJ_1550"/>
    <dbReference type="EnsemblBacteria" id="AAB99580">
        <property type="protein sequence ID" value="AAB99580"/>
        <property type="gene ID" value="MJ_1550"/>
    </dbReference>
    <dbReference type="GeneID" id="1452458"/>
    <dbReference type="KEGG" id="mja:MJ_1550"/>
    <dbReference type="eggNOG" id="arCOG08299">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208221_0_0_2"/>
    <dbReference type="OMA" id="VMWVGGE"/>
    <dbReference type="OrthoDB" id="126201at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107404" description="Uncharacterized protein MJ1550">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <sequence length="61" mass="7106" checksum="52C0B8011E728F12" modified="1996-11-01" version="1">MDKFCPFIKDICKKDKCVMWVGGECRLIMLIDLYISSKIYELYGYENGTLEVEDVDEDEAS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58954</accession>
    <name>NTPTH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Nucleoside-triphosphatase THEP1</fullName>
            <shortName>NTPase THEP1</shortName>
            <ecNumber>3.6.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Nucleoside triphosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1559</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Has nucleotide phosphatase activity towards ATP, GTP, CTP, TTP and UTP. May hydrolyze nucleoside diphosphates with lower efficiency.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THEP1 NTPase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99578" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.6.1.15"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99578.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64494">
        <property type="entry name" value="F64494"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58954"/>
    <dbReference type="SMR" id="Q58954"/>
    <dbReference type="STRING" id="243232.MJ_1559"/>
    <dbReference type="EnsemblBacteria" id="AAB99578">
        <property type="protein sequence ID" value="AAB99578"/>
        <property type="gene ID" value="MJ_1559"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1559"/>
    <dbReference type="eggNOG" id="arCOG01034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_103145_1_1_2"/>
    <dbReference type="InParanoid" id="Q58954"/>
    <dbReference type="OMA" id="GAMEFKC"/>
    <dbReference type="PhylomeDB" id="Q58954"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:nucleoside-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00796">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004948">
        <property type="entry name" value="Nuc-triphosphatase_THEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43146">
        <property type="entry name" value="PTHR43146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03266">
        <property type="entry name" value="NTPase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000146696" description="Nucleoside-triphosphatase THEP1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="170" mass="19088" checksum="48DBF7DA574109F6" modified="2004-10-11" version="2">MRIFITGMPGVGKTTLALKIAEKLKELGYKVGGFITKEIRDGGKRVGFKIITLDTNEETILAYVGDGKIKVGKYAVFIENLDNVGVEAIKRALKDADIIIIDELGAMEFKSRKFSEVVDEVIKSDKPLLATLHRNWVNKFKDKGELYTLTIENREKLFEEILNKILAGLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58965</accession>
    <name>Y1570_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1570</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1570</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.thermoautotrophicum MTH1706.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64496">
        <property type="entry name" value="A64496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871094.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58965"/>
    <dbReference type="STRING" id="243232.MJ_1570"/>
    <dbReference type="EnsemblBacteria" id="AAB99594">
        <property type="protein sequence ID" value="AAB99594"/>
        <property type="gene ID" value="MJ_1570"/>
    </dbReference>
    <dbReference type="GeneID" id="1452478"/>
    <dbReference type="KEGG" id="mja:MJ_1570"/>
    <dbReference type="eggNOG" id="arCOG03159">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174500_0_0_2"/>
    <dbReference type="InParanoid" id="Q58965"/>
    <dbReference type="OMA" id="HLMTERK"/>
    <dbReference type="OrthoDB" id="99698at2157"/>
    <dbReference type="PhylomeDB" id="Q58965"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025937">
        <property type="entry name" value="PDGLE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13190">
        <property type="entry name" value="PDGLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107417" description="Uncharacterized protein MJ1570">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="115" mass="12632" checksum="7DEA63DE6131C6A8" modified="1996-11-01" version="1">MNWQDPLVKKFLYLIVAMVILCPLGILLVWNYGDAWGEWGPEDVAEKVGEDKVSGLLHLADIWSYAPLPDYDIPGWDDPFHASIGYIISAIVGVILCVGAYYALIKIVNPKAAAG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58971</accession>
    <name>LEMA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Protein LemA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">lemA</name>
        <name type="ordered locus">MJ1576</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LemA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64496">
        <property type="entry name" value="G64496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871101.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58971"/>
    <dbReference type="SMR" id="Q58971"/>
    <dbReference type="STRING" id="243232.MJ_1576"/>
    <dbReference type="EnsemblBacteria" id="AAB99598">
        <property type="protein sequence ID" value="AAB99598"/>
        <property type="gene ID" value="MJ_1576"/>
    </dbReference>
    <dbReference type="GeneID" id="1452485"/>
    <dbReference type="KEGG" id="mja:MJ_1576"/>
    <dbReference type="eggNOG" id="arCOG04574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056714_3_0_2"/>
    <dbReference type="InParanoid" id="Q58971"/>
    <dbReference type="OMA" id="VETQYQR"/>
    <dbReference type="OrthoDB" id="78720at2157"/>
    <dbReference type="PhylomeDB" id="Q58971"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023353">
        <property type="entry name" value="LemA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007156">
        <property type="entry name" value="MamQ_LemA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34478">
        <property type="entry name" value="PTHR34478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04011">
        <property type="entry name" value="LemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140478">
        <property type="entry name" value="SSF140478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107422" description="Protein LemA">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="189" mass="21824" checksum="C937C05CE0DBAC9D" modified="1996-11-01" version="1">MLMLLLIIVGLILALIVLGIVIYIVSIYNRFQTLKNGAEATLGQIRVALKKRLDMINQLVEAVKSYASFEKETLTKITELRSSVLKANTAEEIQNIERESRNILGNILVAVENYPELKTSETVKELMDAIKEIEDEIARHRYTYNNIVQEFNTKIDTFPSNIVANLFGFRKMDYLQFEEEIYERPKISF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q59008</accession>
    <name>Y1613_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1613</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1613</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64501">
        <property type="entry name" value="D64501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871138.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59008"/>
    <dbReference type="STRING" id="243232.MJ_1613"/>
    <dbReference type="EnsemblBacteria" id="AAB99633">
        <property type="protein sequence ID" value="AAB99633"/>
        <property type="gene ID" value="MJ_1613"/>
    </dbReference>
    <dbReference type="GeneID" id="1452522"/>
    <dbReference type="KEGG" id="mja:MJ_1613"/>
    <dbReference type="eggNOG" id="arCOG01870">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086012_0_0_2"/>
    <dbReference type="InParanoid" id="Q59008"/>
    <dbReference type="OMA" id="PVPFWDK"/>
    <dbReference type="OrthoDB" id="49651at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107436" description="Uncharacterized protein MJ1613">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <sequence length="255" mass="28552" checksum="495F5F0DE97FCB25" modified="1996-11-01" version="1">MIEKVLEMDDWKAYKIPHTVEIDGMVEETKTLIIEFKNKRKVLSTREGFKEVKYVGNHSIPVPFWDKVHNYKDYENQVLNKIGIKKEDIALLSTGANMDNLAVAKEEFDEFYVVAFTTAGAKHNAIRLGDEEADYIEKDFKTYKIVDGKIVPKEEIGTVNIILITNANLTDGAMARAIITITEAKTNAFQELNIRSTKHPELQATGTGTDNIVVVKGFGSGVDYTGGHTKMGEMIAKAVKRSVIEALIKQDKIKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q59050</accession>
    <name>Y1656_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1656</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1656</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAH family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64506">
        <property type="entry name" value="F64506"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59050"/>
    <dbReference type="SMR" id="Q59050"/>
    <dbReference type="STRING" id="243232.MJ_1656"/>
    <dbReference type="EnsemblBacteria" id="AAB99677">
        <property type="protein sequence ID" value="AAB99677"/>
        <property type="gene ID" value="MJ_1656"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1656"/>
    <dbReference type="eggNOG" id="arCOG00235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028458_4_2_2"/>
    <dbReference type="InParanoid" id="Q59050"/>
    <dbReference type="OMA" id="ANDYIMG"/>
    <dbReference type="PhylomeDB" id="Q59050"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="SSF56529"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156838" description="Uncharacterized protein MJ1656">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="237" mass="26985" checksum="E88847ECDD94DD7C" modified="1996-11-01" version="1">MIISFEKLGEKYKIIDLNLNSIKQKIGDSLNIKEIKPTKIICVGLNYIDHAKELNMEIPEYPIIFLKPTSAIIYNEDYIIRPRISKRVDYEVELAIVIGKKCKNIKKDEANDYIMGYTILNDVTARDLQQKDGQWTRAKSFDTFCPIGPRIVKDIDPMNLNIECRVNGEIKQKSNTKNMIFDVYELVEFVASIMTLYPGDIISTGTPPGVGELKAGDVVECEIEGIGILRNYVKDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q60177</accession>
    <name>PSA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmA</name>
        <name type="ordered locus">MJ0591</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1680" last="1692">
            <title>Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome.</title>
            <authorList>
                <person name="Wilson H.L."/>
                <person name="Ou M.S."/>
                <person name="Aldrich H.C."/>
                <person name="Maupin-Furlow J."/>
            </authorList>
            <dbReference type="PubMed" id="10692374"/>
            <dbReference type="DOI" id="10.1128/JB.182.6.1680-1692.2000"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH PAN</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Mol. Cell" volume="34" first="473" last="484">
            <title>Structural insights into the regulatory particle of the proteasome from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Zhang F."/>
                <person name="Hu M."/>
                <person name="Tian G."/>
                <person name="Zhang P."/>
                <person name="Finley D."/>
                <person name="Jeffrey P.D."/>
                <person name="Shi Y."/>
            </authorList>
            <dbReference type="PubMed" id="19481527"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2009.04.021"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) IN COMPLEX WITH BETA SUBUNIT</scope>
        <scope>GATED STRUCTURE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.jannaschii proteasome is able to cleave oligopeptides after Glu, Asp, Tyr, Phe, Trp, slightly after Arg, but not after Ala. Thus, displays caspase-like and chymotrypsin-like activities and low level of trypsin-like activity.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3 4">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64373">
        <property type="entry name" value="G64373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870095.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3H4P">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.10 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-261"/>
    </dbReference>
    <dbReference type="PDBsum" id="3H4P"/>
    <dbReference type="AlphaFoldDB" id="Q60177"/>
    <dbReference type="SMR" id="Q60177"/>
    <dbReference type="STRING" id="243232.MJ_0591"/>
    <dbReference type="EnsemblBacteria" id="AAB98581">
        <property type="protein sequence ID" value="AAB98581"/>
        <property type="gene ID" value="MJ_0591"/>
    </dbReference>
    <dbReference type="GeneID" id="1451456"/>
    <dbReference type="KEGG" id="mja:MJ_0591"/>
    <dbReference type="eggNOG" id="arCOG00971">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_4_1_2"/>
    <dbReference type="InParanoid" id="Q60177"/>
    <dbReference type="OMA" id="FQVEYAR"/>
    <dbReference type="OrthoDB" id="57654at2157"/>
    <dbReference type="PhylomeDB" id="Q60177"/>
    <dbReference type="EvolutionaryTrace" id="Q60177"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000124174" description="Proteasome subunit alpha">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10692374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="19481527"/>
        </source>
    </evidence>
    <sequence length="261" mass="29321" checksum="93FC7DF277E01AD8" modified="1997-11-01" version="1">MQMVPPSAYDRAITVFSPEGRLYQVEYAREAVRRGTTAIGIACKDGVVLAVDRRITSKLVKIRSIEKIFQIDDHVAAATSGLVADARVLIDRARLEAQIYRLTYGEEISIEMLAKKICDIKQAYTQHGGVRPFGVSLLIAGIDKNEARLFETDPSGALIEYKATAIGSGRPVVMELLEKEYRDDITLDEGLELAITALTKANEDIKPENVDVCIITVKDAQFKKIPVEEIKKLIEKVKKKLNEENKKEEENREETKEKQEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q60263</accession>
    <name>Y3516_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL16</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the M.jannaschii MJ0023/MJ0349/MJ1072/MJ1074/MJ1107/MJECL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64511">
        <property type="entry name" value="H64511"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60263"/>
    <dbReference type="SMR" id="Q60263"/>
    <dbReference type="STRING" id="243232.MJ_ECL16"/>
    <dbReference type="EnsemblBacteria" id="AAC37072">
        <property type="protein sequence ID" value="AAC37072"/>
        <property type="gene ID" value="MJ_ECL16"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_ECL16"/>
    <dbReference type="eggNOG" id="arCOG09652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2748196_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q60263"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107505" description="Uncharacterized protein MJECL16">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="70" mass="8592" checksum="47267115DB4D4E16" modified="1997-11-01" version="1">MSILISNKQFNHGLKDEFATKKDLELLEERILRYVDNKFNQLDKKIDRTFYLLVFFIILWVSREAFFYLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q60264</accession>
    <name>HJA4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable archaeal histone 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL29</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or heterodimer with another histone. Dimers then assemble into higher oligomers, with the DNA wrapped around the protein core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64513">
        <property type="entry name" value="D64513"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890076.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60264"/>
    <dbReference type="SMR" id="Q60264"/>
    <dbReference type="STRING" id="243232.MJ_ECL29"/>
    <dbReference type="EnsemblBacteria" id="AAC37073">
        <property type="protein sequence ID" value="AAC37073"/>
        <property type="gene ID" value="MJ_ECL29"/>
    </dbReference>
    <dbReference type="GeneID" id="1450786"/>
    <dbReference type="KEGG" id="mja:MJ_ECL29"/>
    <dbReference type="eggNOG" id="arCOG02144">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_192667_0_0_2"/>
    <dbReference type="InParanoid" id="Q60264"/>
    <dbReference type="OMA" id="VEWANHA"/>
    <dbReference type="PhylomeDB" id="Q60264"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154990" description="Probable archaeal histone 4">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with DNA" evidence="1">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with DNA" evidence="1">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P19267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="67" mass="7476" checksum="1022FDE19747FB5C" modified="1997-11-01" version="1">MTELPVAPFERILKKVGAERVSRAAAEYLAEAFEEIALEIAKEAVDLAKHAKRKTVKVEDIKLALKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q60283</accession>
    <name>Y3524_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL24</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ParA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64512">
        <property type="entry name" value="G64512"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890071.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60283"/>
    <dbReference type="SMR" id="Q60283"/>
    <dbReference type="STRING" id="243232.MJ_ECL24"/>
    <dbReference type="EnsemblBacteria" id="AAC37095">
        <property type="protein sequence ID" value="AAC37095"/>
        <property type="gene ID" value="MJ_ECL24"/>
    </dbReference>
    <dbReference type="GeneID" id="1450808"/>
    <dbReference type="KEGG" id="mja:MJ_ECL24"/>
    <dbReference type="eggNOG" id="arCOG00586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_6_3_2"/>
    <dbReference type="InParanoid" id="Q60283"/>
    <dbReference type="OMA" id="PKLEAKH"/>
    <dbReference type="OrthoDB" id="72034at2157"/>
    <dbReference type="PhylomeDB" id="Q60283"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000201991" description="Uncharacterized protein MJECL24">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="259" mass="29031" checksum="C637DF1BFF78EFAC" modified="1997-11-01" version="1">MVVISIANQKGGVGKTTIALNLSFTLAEKGYDTLVIDLDPQFNLSFGILGMKLLDYADKNIGILLSKNSVKKKEIEESIIKINDKLDLIPSHLQLSAVEKMLVNAYAREMKLKNIINQIKENYDYIIIDNAPSLGLFLINSLVASDYIIIPCEPSYFSIAGVQLMLDTVEEIKESNLNPKLKVLGFIFNKYSKQSKIPQKRLEQLKQLYPNIPVIGVIPRTITVEKAEREGKPVFKFDANNPASVAFSELAEWVIENVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q60284</accession>
    <name>Y3525_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL25</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL25</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64512">
        <property type="entry name" value="H64512"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890072.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60284"/>
    <dbReference type="EnsemblBacteria" id="AAC37096">
        <property type="protein sequence ID" value="AAC37096"/>
        <property type="gene ID" value="MJ_ECL25"/>
    </dbReference>
    <dbReference type="GeneID" id="1450809"/>
    <dbReference type="KEGG" id="mja:MJ_ECL25"/>
    <dbReference type="eggNOG" id="arCOG05095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2165282_0_0_2"/>
    <dbReference type="InParanoid" id="Q60284"/>
    <dbReference type="OMA" id="REYMGRF"/>
    <dbReference type="PhylomeDB" id="Q60284"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107512" description="Uncharacterized protein MJECL25">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <sequence length="110" mass="12650" checksum="828470CFA71E24C9" modified="1997-11-01" version="1">MSNDDLNALKKLKEISSGTVKQITSLPKKEPQEEKMSKTLRIKNTTHEKIIEMYGKKVGSQGEVVDKGVAVLYALWKILPEEQFKRVVKLAEEDRFEEFADRLGIEIKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q60287</accession>
    <name>Y3528_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL28</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64513">
        <property type="entry name" value="C64513"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890075.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60287"/>
    <dbReference type="PRIDE" id="Q60287"/>
    <dbReference type="EnsemblBacteria" id="AAC37099">
        <property type="protein sequence ID" value="AAC37099"/>
        <property type="gene ID" value="MJ_ECL28"/>
    </dbReference>
    <dbReference type="GeneID" id="1450812"/>
    <dbReference type="KEGG" id="mja:MJ_ECL28"/>
    <dbReference type="eggNOG" id="arCOG07629">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_271775_0_0_2"/>
    <dbReference type="OMA" id="NIVAYLW"/>
    <dbReference type="OrthoDB" id="132879at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107514" description="Uncharacterized protein MJECL28">
        <location>
            <begin position="1"/>
            <end position="1272"/>
        </location>
    </feature>
    <sequence length="1272" mass="149439" checksum="88E7AE8C48629DEA" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q60326</accession>
    <name>APGM2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2</fullName>
            <shortName>BPG-independent PGAM 2</shortName>
            <shortName>Phosphoglyceromutase 2</shortName>
            <shortName>aPGAM 2</shortName>
            <ecNumber>5.4.2.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>aPGAM-Mj2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">apgM2</name>
        <name type="ordered locus">MJ0010</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="FEBS Lett." volume="517" first="190" last="194">
            <title>A divergent archaeal member of the alkaline phosphatase binuclear metalloenzyme superfamily has phosphoglycerate mutase activity.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="12062435"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(02)02619-4"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64301">
        <property type="entry name" value="B64301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869503.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60326"/>
    <dbReference type="SMR" id="Q60326"/>
    <dbReference type="STRING" id="243232.MJ_0010"/>
    <dbReference type="EnsemblBacteria" id="AAB97991">
        <property type="protein sequence ID" value="AAB97991"/>
        <property type="gene ID" value="MJ_0010"/>
    </dbReference>
    <dbReference type="GeneID" id="1450849"/>
    <dbReference type="KEGG" id="mja:MJ_0010"/>
    <dbReference type="eggNOG" id="arCOG01696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034906_1_0_2"/>
    <dbReference type="InParanoid" id="Q60326"/>
    <dbReference type="OMA" id="HIHTKEP"/>
    <dbReference type="OrthoDB" id="17268at2157"/>
    <dbReference type="PhylomeDB" id="Q60326"/>
    <dbReference type="BRENDA" id="5.4.2.12">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="PTHR31209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138135" description="2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2">
        <location>
            <begin position="1"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="12062435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="428" mass="49350" checksum="7107678524B1C168" modified="1997-11-01" version="1">MRAILILLDGLGDRASEILNNKTPLQFAKTPNLDRLAENGMCGLMTTYKEGIPLGTEVAHFLLWGYSLEEFPGRGVIEALGEDIEIEKNAIYLRASLGFVKKDEKGFLVIDRRTKDISREEIEKLVDSLPTCVDGYKFELFYSFDVHFILKIKERNGWISDKISDSDPFYKNRYVMKVKAIRELCKSEVEYSKAKDTARALNKYLLNVYKILQNHKINRKRRKLEKMPANFLLTKWASRYKRVESFKEKWGMNAVILAESSLFKGLAKFLGMDFIKIESFEEGIDLIPELDYDFIHLHTKETDEAAHTKNPLNKVKVIEKIDKLIGNLKLREDDLLIITADHSTPSVGNLIHSGESVPILFYGKNVRVDNVKEFNEISCSNGHLRIRGEELMHLILNYTDRALLYGLRSGDRLRYYIPKDDEIDLLEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q60337</accession>
    <name>THIL_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="1">TMP kinase</shortName>
            <shortName evidence="1">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="1">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiL</name>
        <name type="ordered locus">MJ0028</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + thiamine phosphate = ADP + thiamine diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64303">
        <property type="entry name" value="D64303"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869520.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60337"/>
    <dbReference type="SMR" id="Q60337"/>
    <dbReference type="STRING" id="243232.MJ_0028"/>
    <dbReference type="TCDB" id="8.A.159.1.2">
        <property type="family name" value="the march ubiquitin ligase (march) family"/>
    </dbReference>
    <dbReference type="DNASU" id="1450866"/>
    <dbReference type="EnsemblBacteria" id="AAB98008">
        <property type="protein sequence ID" value="AAB98008"/>
        <property type="gene ID" value="MJ_0028"/>
    </dbReference>
    <dbReference type="GeneID" id="1450866"/>
    <dbReference type="KEGG" id="mja:MJ_0028"/>
    <dbReference type="eggNOG" id="arCOG00638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046964_2_0_2"/>
    <dbReference type="InParanoid" id="Q60337"/>
    <dbReference type="OMA" id="HFRRDWS"/>
    <dbReference type="OrthoDB" id="104636at2157"/>
    <dbReference type="PhylomeDB" id="Q60337"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01379">
        <property type="entry name" value="thiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000096201" description="Thiamine-monophosphate kinase">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="121"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <sequence length="319" mass="36160" checksum="92607D5AD59A723F" modified="1997-11-01" version="1">MDEMKVIEIIKKTLKFSNENIVKGIDDDCAIIKIDENFYLVATTDMMVKKAHIPSILSPYEIGGRILTANVSDIASMGAKPLAFLVSISLSKEEANEKFIKELYSGLDDFSKLYDCPVVGGDTNRGDELILSGTAFGITDNPIYRRGKVGDDICVTNDLGRVYCALTLYYMLKENKISYKEFERLCQKYPKIIEKLRKPIARIKEGLLMNKLINGCCDISDGLGKEITYFKNFEIYSDRIFKLIPEDVIEFCDAFNLNPIKVALNSGEEFELLFTTSKFNKVKDSLKGYSKIYKIGKIIEDGQFIDGEEFYGGGYIHKW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q60349</accession>
    <name>Y034_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0051 protein MJ0034</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0034</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0051 (ycf24) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64304">
        <property type="entry name" value="B64304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869525.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60349"/>
    <dbReference type="SMR" id="Q60349"/>
    <dbReference type="STRING" id="243232.MJ_0034"/>
    <dbReference type="EnsemblBacteria" id="AAB98015">
        <property type="protein sequence ID" value="AAB98015"/>
        <property type="gene ID" value="MJ_0034"/>
    </dbReference>
    <dbReference type="GeneID" id="1450872"/>
    <dbReference type="KEGG" id="mja:MJ_0034"/>
    <dbReference type="eggNOG" id="arCOG01716">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_894122_0_0_2"/>
    <dbReference type="InParanoid" id="Q60349"/>
    <dbReference type="OMA" id="PVHLCFG"/>
    <dbReference type="OrthoDB" id="35631at2157"/>
    <dbReference type="PhylomeDB" id="Q60349"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="SSF101960"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147379" description="UPF0051 protein MJ0034">
        <location>
            <begin position="1"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="316" mass="35022" checksum="3657AC25CBE473D1" modified="1997-11-01" version="1">MSIKEELMEIIEAIKYTSEKPEEIVHGKGPRIIVKESRIIDVQGDEGIILEGKEEDGKIKAKIIVKKGYKFKYPIHMCFGITEENISQIIDVEIILEEDSSISLMSHCSFPKGKGIKHIMNGIIKIGKNAKFSYNEFHYHGMDGDILVKPTVKVEIDEGGIYISNFTLTKGRIGTLDIEQEIIAKKDAIIDITTRTYAIKEDVVKVNEVVKLNGENAKCIIKSRGAAMDNSKISLKLKIEGNAPYSKGHIDCAEIVKGNAEVESIPIVVVRDDKARITHEAAIGSVDKKQLETLMAKGLDEDEATEIIVKGMIGDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q60350</accession>
    <name>Y035_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ0035</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0035</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64304">
        <property type="entry name" value="C64304"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60350"/>
    <dbReference type="SMR" id="Q60350"/>
    <dbReference type="STRING" id="243232.MJ_0035"/>
    <dbReference type="EnsemblBacteria" id="AAB98016">
        <property type="protein sequence ID" value="AAB98016"/>
        <property type="gene ID" value="MJ_0035"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0035"/>
    <dbReference type="eggNOG" id="arCOG04236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q60350"/>
    <dbReference type="OMA" id="ILITHHE"/>
    <dbReference type="PhylomeDB" id="Q60350"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="PTHR43204"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093216" description="Uncharacterized ABC transporter ATP-binding protein MJ0035">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="7"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="250" mass="28258" checksum="B49671A4698ABED1" modified="1997-11-01" version="1">MVSIMLLKVEDLHVYRGNREILKGVNLTVEENEIHAIIGPNGAGKSTLAYTIMGISGYKPTKGRIIFKGVDIIDKNITERARMGMTLAWQEPARFEGIKVKNYLMLGMNEKYKKDKEIAEEKIREALKLVNLDPDKYLDRYVDETLSGGERKRIELASIICMEPDLAILDEPDSGIDIVSFDEIKRVFDYLKDKGCSLLVITHREELAEHADRVSLICAGEVIKSGDPKEVGEFYKKECGKCYKKVPDGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q60353</accession>
    <name>Y045_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0045</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0045</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64305">
        <property type="entry name" value="E64305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869536.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60353"/>
    <dbReference type="STRING" id="243232.MJ_0045"/>
    <dbReference type="PRIDE" id="Q60353"/>
    <dbReference type="EnsemblBacteria" id="AAB98025">
        <property type="protein sequence ID" value="AAB98025"/>
        <property type="gene ID" value="MJ_0045"/>
    </dbReference>
    <dbReference type="GeneID" id="1450883"/>
    <dbReference type="KEGG" id="mja:MJ_0045"/>
    <dbReference type="eggNOG" id="arCOG04855">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077876_1_0_2"/>
    <dbReference type="InParanoid" id="Q60353"/>
    <dbReference type="OMA" id="VPWGRSF"/>
    <dbReference type="OrthoDB" id="95021at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106667" description="Uncharacterized protein MJ0045">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <sequence length="221" mass="26679" checksum="B9873BF0B1171336" modified="1997-11-01" version="1">MRYLNLKDTVLLGRNFNEYVRMFNLNEDLLSNKILDVASGVSSFCAEGNKKGYNITSSDKIYNLKPEEIEEKCKKDLDFMEKHLRGMFKNNFNWNEFKTVDEWKKTRERTYKTFIEDYKTNRKRYIYTTYPKTNFKDDEFAISLVGHFLLLYDNILNYQFHKETIDELLRISEEIRIFPILNLRGEKSIFLDKILKEYKARIEKTDYEFMKGGNKVLIIRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q60379</accession>
    <name>Y073_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0073</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0073</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64309">
        <property type="entry name" value="A64309"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60379"/>
    <dbReference type="SMR" id="Q60379"/>
    <dbReference type="STRING" id="243232.MJ_0073"/>
    <dbReference type="EnsemblBacteria" id="AAB98055">
        <property type="protein sequence ID" value="AAB98055"/>
        <property type="gene ID" value="MJ_0073"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0073"/>
    <dbReference type="eggNOG" id="arCOG05114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392907_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q60379"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106681" description="Uncharacterized protein MJ0073">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <sequence length="93" mass="10335" checksum="749F111B601BBD2F" modified="1997-11-01" version="1">MKVDREKYGSEKIAKLKILEELKKENPNKKIIAIGNGNNDELLLKNADLGICVIGDEGAWSKTILSSDIVVKDINDALDLLLNENRLKATSRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2022-05-25" version="24" xmlns="http://uniprot.org/uniprot">
    <accession>P0CW38</accession>
    <name>VAPB4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative antitoxin VapB4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vapB4</name>
        <name type="ordered locus">MJ1319.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>IDENTIFICATION</scope>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possibly the antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is VapC4.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P0CW38"/>
    <dbReference type="SMR" id="P0CW38"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000408088" description="Putative antitoxin VapB4">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="57" mass="6797" checksum="4CD6D05CE6D4950A" modified="2011-05-03" version="1">MQLLYDLTKMNYSALYGEGRYLRIPAPIHYADKFVKALGKNWKIDEELLKHGFLYFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>P54021</accession>
    <name>RS11_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps11</name>
        <name type="ordered locus">MJ0191</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64323">
        <property type="entry name" value="H64323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869686.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54021"/>
    <dbReference type="SMR" id="P54021"/>
    <dbReference type="STRING" id="243232.MJ_0191"/>
    <dbReference type="EnsemblBacteria" id="AAB98171">
        <property type="protein sequence ID" value="AAB98171"/>
        <property type="gene ID" value="MJ_0191"/>
    </dbReference>
    <dbReference type="GeneID" id="1451039"/>
    <dbReference type="KEGG" id="mja:MJ_0191"/>
    <dbReference type="eggNOG" id="arCOG04240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072439_6_1_2"/>
    <dbReference type="InParanoid" id="P54021"/>
    <dbReference type="OMA" id="KWGVAHI"/>
    <dbReference type="OrthoDB" id="114285at2157"/>
    <dbReference type="PhylomeDB" id="P54021"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048027">
        <property type="term" value="F:mRNA 5'-UTR binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_S11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_S11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_S11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="PTHR11759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000123271" description="30S ribosomal protein S11">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <sequence length="129" mass="13996" checksum="67C6C99468E2F75F" modified="1996-10-01" version="1">MAEQKKEKWGIVHIYSSYNNTIIHATDITGAETIARVSGGRVTRNQRDEGSPYAAMQAAFKLAEVLKERGIENIHIKVRAPGGSGQKNPGPGAQAAIRALARAGLRIGRIEDVTPVPHDGTTPKKRFKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>P54032</accession>
    <name>RS24_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S24e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps24e</name>
        <name type="ordered locus">MJ0394</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64349">
        <property type="entry name" value="B64349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869893.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54032"/>
    <dbReference type="SMR" id="P54032"/>
    <dbReference type="STRING" id="243232.MJ_0394"/>
    <dbReference type="PRIDE" id="P54032"/>
    <dbReference type="EnsemblBacteria" id="AAB98384">
        <property type="protein sequence ID" value="AAB98384"/>
        <property type="gene ID" value="MJ_0394"/>
    </dbReference>
    <dbReference type="GeneID" id="1451251"/>
    <dbReference type="KEGG" id="mja:MJ_0394"/>
    <dbReference type="eggNOG" id="arCOG04182">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107248_3_1_2"/>
    <dbReference type="InParanoid" id="P54032"/>
    <dbReference type="OMA" id="QYIRSYF"/>
    <dbReference type="OrthoDB" id="124805at2157"/>
    <dbReference type="PhylomeDB" id="P54032"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_L23/L15e_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_S24e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_S24e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="PTHR10496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="SSF54189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000137643" description="30S ribosomal protein S24e">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <sequence length="101" mass="11842" checksum="F84A3BE414F09036" modified="1996-10-01" version="1">MEIKILSERYNPLLKRKEYRFIVDHDGPTPTFKDVKLKLAAILNANKDLLIVEKIVEEAGMQRARGYAKLYDNEEMLKLVEREHILRKNKIEEETAAEEGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>P54051</accession>
    <name>RL37A_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L37Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L43e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl37ae</name>
        <name type="ordered locus">MJ0593</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64374">
        <property type="entry name" value="A64374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870097.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54051"/>
    <dbReference type="SMR" id="P54051"/>
    <dbReference type="STRING" id="243232.MJ_0593"/>
    <dbReference type="EnsemblBacteria" id="AAB98587">
        <property type="protein sequence ID" value="AAB98587"/>
        <property type="gene ID" value="MJ_0593"/>
    </dbReference>
    <dbReference type="GeneID" id="8805446"/>
    <dbReference type="KEGG" id="mja:MJ_0593"/>
    <dbReference type="eggNOG" id="arCOG04208">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141199_2_0_2"/>
    <dbReference type="InParanoid" id="P54051"/>
    <dbReference type="OMA" id="ISTGIWQ"/>
    <dbReference type="OrthoDB" id="122290at2157"/>
    <dbReference type="PhylomeDB" id="P54051"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_L37Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_L37ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_L37ae/L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000139843" description="50S ribosomal protein L37Ae">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <sequence length="92" mass="10183" checksum="63DC201CCE508F9D" modified="1996-10-01" version="1">MFSHTKKVGPTGRFGPRYGLKIRVRVRDVEIKAKKKYKCPVCGFPKLKRASTSIWVCGKCGAKIAGGAYTPETGAGKAVMKAIRRIVERKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P54053</accession>
    <name>RL34_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L34e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl34e</name>
        <name type="ordered locus">MJ0655</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64381">
        <property type="entry name" value="G64381"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870160.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54053"/>
    <dbReference type="SMR" id="P54053"/>
    <dbReference type="STRING" id="243232.MJ_0655"/>
    <dbReference type="EnsemblBacteria" id="AAB98650">
        <property type="protein sequence ID" value="AAB98650"/>
        <property type="gene ID" value="MJ_0655"/>
    </dbReference>
    <dbReference type="GeneID" id="1451521"/>
    <dbReference type="KEGG" id="mja:MJ_0655"/>
    <dbReference type="eggNOG" id="arCOG04168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118652_2_0_2"/>
    <dbReference type="InParanoid" id="P54053"/>
    <dbReference type="OMA" id="SWRRKYV"/>
    <dbReference type="OrthoDB" id="119402at2157"/>
    <dbReference type="PhylomeDB" id="P54053"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00349">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_L34Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_L34e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="PTHR10759"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000131848" description="50S ribosomal protein L34e">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="89" mass="10524" checksum="D86DC72F53BFA0C6" modified="1996-10-01" version="1">MPAPRYRSRSYRRIYRRTPGGRIVIHYKRRKPGKPKCAICGAELHGVPRGRPVEIRKLPKSQRRPERPYGGYLCPRCLKRLMIQKARNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>P58415</accession>
    <name>COMD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Sulfopyruvate decarboxylase subunit alpha</fullName>
            <ecNumber evidence="1">4.1.1.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">comD</name>
        <name type="ordered locus">MJ0255.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="4862" last="4867">
            <title>Identification of the gene encoding sulfopyruvate decarboxylase, an enzyme involved in biosynthesis of coenzyme M.</title>
            <authorList>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10940029"/>
            <dbReference type="DOI" id="10.1128/JB.182.17.4862-4867.2000"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the coenzyme M (2-mercaptoethanesulfonic acid). Catalyzes the decarboxylation of sulfopyruvate to sulfoacetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-sulfopyruvate + H(+) = CO2 + sulfoacetaldehyde</text>
            <dbReference type="Rhea" id="RHEA:20948"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57940"/>
            <dbReference type="ChEBI" id="CHEBI:58246"/>
            <dbReference type="EC" id="4.1.1.79"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by oxygen when heated in air at 80 degrees Celsius. The enzyme is reactivated by addition of dithionite.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">0.64 mM for 3-sulfopyruvate</KM>
            <Vmax evidence="1">52.0 umol/min/mg enzyme</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="4">Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterododecamer composed of 6 subunits alpha and 6 subunits beta.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ComD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.79" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496437.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58415"/>
    <dbReference type="SMR" id="P58415"/>
    <dbReference type="GeneID" id="27929948"/>
    <dbReference type="InParanoid" id="P58415"/>
    <dbReference type="OMA" id="IPMGQWT"/>
    <dbReference type="OrthoDB" id="80332at2157"/>
    <dbReference type="PhylomeDB" id="P58415"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-2265"/>
    <dbReference type="BRENDA" id="4.1.1.79">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="P58415"/>
    <dbReference type="UniPathway" id="UPA00355">
        <property type="reaction ID" value="UER00472"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022502">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03845">
        <property type="entry name" value="sulfopyru_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000090837" description="Sulfopyruvate decarboxylase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="10940029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="10940029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="10940029"/>
        </source>
    </evidence>
    <sequence length="169" mass="19059" checksum="2FB248306F7D211C" modified="2001-12-05" version="1">MRGSLAIYNALKDSNIDFICSVPCANLKNLLKLIEEDKNIINIPATREEEAFGICAGAYLAGKKTAILMQNSGIGNSINAIASLYKTFQIPTLLIISHRGDLKEQIPAQIPMGRWIEKLLDVCEIPTYKPKTPEEAYKLIKYASSYMYKISYPVALLFDALYWEYDLEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="58" xmlns="http://uniprot.org/uniprot">
    <accession>P81321</accession>
    <name>Y80A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0808.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0808.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870319.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81321"/>
    <dbReference type="SMR" id="P81321"/>
    <dbReference type="EnsemblBacteria" id="AAB98820">
        <property type="protein sequence ID" value="AAB98820"/>
        <property type="gene ID" value="MJ_0808.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451691"/>
    <dbReference type="KEGG" id="mja:MJ_0808.1"/>
    <dbReference type="HOGENOM" id="CLU_1850646_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107058" description="Uncharacterized protein MJ0808.1">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <sequence length="138" mass="16750" checksum="DA087F74CA5A064D" modified="1998-07-15" version="1">MLKEIKNDYDKIREKMTQKIQELNQQITQIKKQIQIIEQNNLSDQQNQTIQKIKRQIYSIEFDILRVESNRSNMIYSKTFEDMCEYLDSHSGIGRIFAESFMREIEKNIQLMKQLVMMEDQIIKIKQEIRMIEKDLKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q57553</accession>
    <name>Y088_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0088</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0088</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="H64310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869580.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57553"/>
    <dbReference type="STRING" id="243232.MJ_0088"/>
    <dbReference type="EnsemblBacteria" id="AAB98075">
        <property type="protein sequence ID" value="AAB98075"/>
        <property type="gene ID" value="MJ_0088"/>
    </dbReference>
    <dbReference type="GeneID" id="1450927"/>
    <dbReference type="KEGG" id="mja:MJ_0088"/>
    <dbReference type="eggNOG" id="arCOG12714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1492985_0_0_2"/>
    <dbReference type="OMA" id="IMGYIRR"/>
    <dbReference type="OrthoDB" id="134049at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106688" description="Uncharacterized protein MJ0088">
        <location>
            <begin position="1"/>
            <end position="180"/>
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    </feature>
    <sequence length="180" mass="21797" checksum="CD2C47D358C5649F" modified="1997-11-01" version="1">MPNIWGGMLEKTVKITFIFESEKRLNWEGYVNYLIFAHYFKLIYILLRNNKELLERILKDSLDNKEDYHKLGKYLHHDIMGYIRRHGYLSFLPFTPLPFYKELCEKFMTKIPEKPIIDYKCIERIENNKIIFEFEGEEECLRCLMYLKDSSIDESIKVIKSEEMVVKILEILLYILHNLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q57556</accession>
    <name>Y091_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized membrane protein MJ0091</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0091</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
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        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB98072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C64311"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869583.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3V5S">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="A=1-302"/>
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    <dbReference type="PDB" id="3V5U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-302"/>
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    <dbReference type="PDB" id="5HWX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=1-301"/>
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    <dbReference type="PDB" id="5HWY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-300"/>
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    <dbReference type="PDB" id="5HXC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-302"/>
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    <dbReference type="PDB" id="5HXE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.29 A"/>
        <property type="chains" value="A=1-302"/>
    </dbReference>
    <dbReference type="PDB" id="5HXH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-300"/>
    </dbReference>
    <dbReference type="PDB" id="5HXR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.46 A"/>
        <property type="chains" value="A=1-300"/>
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    <dbReference type="PDB" id="5HXS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.79 A"/>
        <property type="chains" value="A=1-300"/>
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    <dbReference type="PDB" id="5HYA">
        <property type="method" value="X-ray"/>
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        <property type="chains" value="A=1-302"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.65 A"/>
        <property type="chains" value="A=1-302"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.41 A"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=1-301"/>
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    <dbReference type="PDB" id="5JDL">
        <property type="method" value="X-ray"/>
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        <property type="chains" value="A=1-301"/>
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    <dbReference type="PDB" id="5JDM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.56 A"/>
        <property type="chains" value="A=1-302"/>
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        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-302"/>
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        <property type="resolution" value="2.50 A"/>
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    <dbReference type="PDBsum" id="3V5S"/>
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    <dbReference type="PDBsum" id="5JDN"/>
    <dbReference type="PDBsum" id="5JDQ"/>
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    <dbReference type="SMR" id="Q57556"/>
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    <dbReference type="TCDB" id="2.A.19.5.3">
        <property type="family name" value="the ca(2+):cation antiporter (caca) family"/>
    </dbReference>
    <dbReference type="DNASU" id="1450930"/>
    <dbReference type="EnsemblBacteria" id="AAB98072">
        <property type="protein sequence ID" value="AAB98072"/>
        <property type="gene ID" value="MJ_0091"/>
    </dbReference>
    <dbReference type="GeneID" id="1450930"/>
    <dbReference type="KEGG" id="mja:MJ_0091"/>
    <dbReference type="eggNOG" id="arCOG02881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007948_0_1_2"/>
    <dbReference type="InParanoid" id="Q57556"/>
    <dbReference type="OMA" id="TRDYGVM"/>
    <dbReference type="OrthoDB" id="45269at2157"/>
    <dbReference type="PhylomeDB" id="Q57556"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
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        <property type="term" value="P:cellular calcium ion homeostasis"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
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        <property type="entry name" value="NaCa_Exmemb"/>
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        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
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        <property type="entry name" value="PTHR10846"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="TIGR00367"/>
        <property type="match status" value="1"/>
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000209509" description="Uncharacterized membrane protein MJ0091">
        <location>
            <begin position="1"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
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            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="247"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="2"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="38"/>
            <end position="43"/>
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    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="45"/>
            <end position="63"/>
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    </feature>
    <feature type="helix" evidence="4">
        <location>
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            <end position="83"/>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="85"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="99"/>
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        </location>
    </feature>
    <feature type="turn" evidence="4">
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            <end position="121"/>
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    <feature type="helix" evidence="4">
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    <feature type="helix" evidence="3">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="198"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="205"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="226"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="244"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5HWY"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="5HYA"/>
        </source>
    </evidence>
    <sequence length="302" mass="32213" checksum="3D4F8373ED375879" modified="1997-11-01" version="1">MLILGVGYFLLGLILLYYGSDWFVLGSERIARHFNVSNFVIGATVMAIGTSLPEILTSAYASYMHAPGISIGNAIGSCICNIGLVLGLSAIISPIIVDKNLQKNILVYLLFVIFAAVIGIDGFSWIDGVVLLILFIIYLRWTVKNGSAEIEENNDKNNPSVVFSLVLLIIGLIGVLVGAELFVDGAKKIALALDISDKVIGFTLVAFGTSLPELMVSLAAAKRNLGGMVLGNVIGSNIADIGGALAVGSLFMHLPAENVQMAVLVIMSLLLYLFAKYSKIGRWQGILFLALYIIAIASLRMG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q57577</accession>
    <name>ACDD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit delta</fullName>
            <shortName evidence="1">ACDS complex subunit delta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Corrinoid/iron-sulfur component small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhD</name>
        <name type="ordered locus">MJ0113</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). Probably maintains the overall quaternary structure of the ACDS complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdhD family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64314">
        <property type="entry name" value="A64314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57577"/>
    <dbReference type="SMR" id="Q57577"/>
    <dbReference type="STRING" id="243232.MJ_0113"/>
    <dbReference type="PRIDE" id="Q57577"/>
    <dbReference type="EnsemblBacteria" id="AAB98094">
        <property type="protein sequence ID" value="AAB98094"/>
        <property type="gene ID" value="MJ_0113"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0113"/>
    <dbReference type="eggNOG" id="arCOG01980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040403_0_0_2"/>
    <dbReference type="InParanoid" id="Q57577"/>
    <dbReference type="OMA" id="VMDPTTC"/>
    <dbReference type="PhylomeDB" id="Q57577"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01135">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004486">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00381">
        <property type="entry name" value="cdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155112" description="Acetyl-CoA decarbonylase/synthase complex subunit delta">
        <location>
            <begin position="1"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01135"/>
        </source>
    </evidence>
    <sequence length="405" mass="45344" checksum="AAD33B438559E911" modified="1996-11-01" version="1">MIYNDRLGEVMDLNTLIKIIEKVGRIEIEDIKITADELIINIPSAPPIVIPQTPSIKEKLAEEGIIEIKDVPELDWEPPVEKYPGYIREVQFGKPKSEGGRGKVVKIGGQRALYRFEEPQPNPPVVTFDIFDIPMPGLPKPIRQFFQDVMEDPCEWAKKCVKEFGADMITIHHISTDPKIKDKSPKEAAKLMEDLLQAVDVPFVIGGSGNPQKDPLVLEACAEVAEGDRCLLASANLELDYKKIVDAAMKYDHNVLAWSIMDPNMARDLNRKLVEAGLDPNRIVMDPTTCALGYGIEFSINAMVRLRLNGLKGDELVNMPMSSGTTNAIGAREAWMNNPEWGPREYRLPLWEITTGITMMMCGVDLFMMLNPISVKTLKEIGKTLTTKPGEVKLNTNNYEWIVSP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57586</accession>
    <name>R15PI_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose 1,5-bisphosphate isomerase</fullName>
            <shortName evidence="1">R15P isomerase</shortName>
            <shortName evidence="1">R15Pi</shortName>
            <ecNumber evidence="1">5.3.1.29</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribulose 1,5-bisphosphate synthase</fullName>
            <shortName evidence="1">RuBP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0122</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization of ribose 1,5-bisphosphate (R15P) to ribulose 1,5-bisphosphate (RuBP), the CO(2) acceptor and substrate for RubisCO. Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and RubisCO.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>alpha-D-ribose 1,5-bisphosphate = D-ribulose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:32243"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="5.3.1.29"/>
        </reaction>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction proceeds via a cis-phosphoenolate intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the eIF-2B alpha/beta/delta subunits family. R15P isomerase subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.29" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64315">
        <property type="entry name" value="B64315"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57586"/>
    <dbReference type="SMR" id="Q57586"/>
    <dbReference type="STRING" id="243232.MJ_0122"/>
    <dbReference type="EnsemblBacteria" id="AAB98103">
        <property type="protein sequence ID" value="AAB98103"/>
        <property type="gene ID" value="MJ_0122"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0122"/>
    <dbReference type="eggNOG" id="arCOG01124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016218_2_1_2"/>
    <dbReference type="InParanoid" id="Q57586"/>
    <dbReference type="OMA" id="GDVIMTH"/>
    <dbReference type="PhylomeDB" id="Q57586"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043917">
        <property type="term" value="F:ribose 1,5-bisphosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02230">
        <property type="entry name" value="R15P_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005250">
        <property type="entry name" value="Ribulose_e2b2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00511">
        <property type="entry name" value="ribulose_e2b2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156103" description="Ribose 1,5-bisphosphate isomerase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="208"/>
            <end position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="308" mass="34518" checksum="EC2BAE3E061E4FCE" modified="1996-11-01" version="1">MVFKMSEMDIIKETYEKIKNMEIRGAGRIGRAAAKALKEYALKISHLNEEEFKNKMREAGNILISARPTAVSLPNVVKYVLKGLNEENPKERVIERADEFINSSLKAIENIGKFGANRIKDGDTILTHCNSEAAISVIKTAYDEGKDIKVFCTETRPRNQGYLTAKTLYDYGIDVTLIVDSAVRYFIKEIDIVVVGADAITANGCLVNKIGTSQIALIANESRVPFLTAAETYKFHPKTIVGELIEIEERSPEEVAVFEDKYKGIKIRNPAFDVTPAKYIDAIITEVGLIPPQGAWYIIEKYFGWLEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q57615</accession>
    <name>PTR1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>HTH-type transcriptional regulator Ptr1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ptr1</name>
        <name type="ordered locus">MJ0151</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="EMBO J." volume="20" first="146" last="156">
            <title>A thermostable platform for transcriptional regulation: the DNA-binding properties of two Lrp homologs from the hyperthermophilic archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Ouhammouch M."/>
                <person name="Geiduschek E.P."/>
            </authorList>
            <dbReference type="PubMed" id="11226165"/>
            <dbReference type="DOI" id="10.1093/emboj/20.1.146"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="function">
        <text>Participates in positive as well as negative regulation of transcription. Binds to its own promoter.</text>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64318">
        <property type="entry name" value="H64318"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869646.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57615"/>
    <dbReference type="SMR" id="Q57615"/>
    <dbReference type="STRING" id="243232.MJ_0151"/>
    <dbReference type="EnsemblBacteria" id="AAB98133">
        <property type="protein sequence ID" value="AAB98133"/>
        <property type="gene ID" value="MJ_0151"/>
    </dbReference>
    <dbReference type="GeneID" id="1450995"/>
    <dbReference type="KEGG" id="mja:MJ_0151"/>
    <dbReference type="eggNOG" id="arCOG01580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091233_5_2_2"/>
    <dbReference type="InParanoid" id="Q57615"/>
    <dbReference type="OMA" id="NIFCKIR"/>
    <dbReference type="OrthoDB" id="97695at2157"/>
    <dbReference type="PhylomeDB" id="Q57615"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="SSF54909"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0010">Activator</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000111752" description="HTH-type transcriptional regulator Ptr1">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="HTH asnC-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00319"/>
        </source>
    </evidence>
    <sequence length="148" mass="16633" checksum="B3AAAC4B34A39982" modified="1996-11-01" version="1">MLDRIDLKILRILNGNARKSFREIGRELGISEGTVRNRVKRLTEKGIITGFHASINPKNLGFEVVAILGLYIKPSKVEETLNKLKELDEIVELYQTTGEYDAVCIAILKDIESLGKFLAEKIYPLVNVNGCKVTLVLRTFKDGSKMPI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q57622</accession>
    <name>Y158_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0425 pyridoxal phosphate-dependent protein MJ0158</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0158</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochemistry" volume="44" first="13315" last="13327">
            <title>Structural and functional investigation of a putative archaeal selenocysteine synthase.</title>
            <authorList>
                <person name="Kaiser J.T."/>
                <person name="Gromadski K."/>
                <person name="Rother M."/>
                <person name="Engelhardt H."/>
                <person name="Rodnina M.V."/>
                <person name="Wahl M.C."/>
            </authorList>
            <dbReference type="PubMed" id="16201757"/>
            <dbReference type="DOI" id="10.1021/bi051110r"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF APOPROTEIN AND COMPLEX WITH COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>PYRIDOXAL PHOSPHATE AT LYS-208</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="mass spectrometry" mass="41886.0" method="Unknown" evidence="1"/>
    <comment type="mass spectrometry" mass="42114.0" method="Unknown" evidence="1">
        <text>The measured mass is that of the PLP-bound protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0425 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Despite a certain similarity to SelA, PubMed:16201757 reported that this protein does not harbor SelA activity, i.e. it fails to bind to Ser-tRNA(Sec) and is not able to convert this substrate to selenocysteinyl-tRNA(Sec). It also does not convert O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64319">
        <property type="entry name" value="G64319"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869653.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2AEU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=1-374"/>
    </dbReference>
    <dbReference type="PDB" id="2AEV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-374"/>
    </dbReference>
    <dbReference type="PDBsum" id="2AEU"/>
    <dbReference type="PDBsum" id="2AEV"/>
    <dbReference type="AlphaFoldDB" id="Q57622"/>
    <dbReference type="SMR" id="Q57622"/>
    <dbReference type="STRING" id="243232.MJ_0158"/>
    <dbReference type="EnsemblBacteria" id="AAB98142">
        <property type="protein sequence ID" value="AAB98142"/>
        <property type="gene ID" value="MJ_0158"/>
    </dbReference>
    <dbReference type="GeneID" id="1451005"/>
    <dbReference type="KEGG" id="mja:MJ_0158"/>
    <dbReference type="eggNOG" id="arCOG00114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055443_0_0_2"/>
    <dbReference type="InParanoid" id="Q57622"/>
    <dbReference type="OMA" id="RIDFCSK"/>
    <dbReference type="OrthoDB" id="37272at2157"/>
    <dbReference type="PhylomeDB" id="Q57622"/>
    <dbReference type="EvolutionaryTrace" id="Q57622"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004125">
        <property type="term" value="F:L-seryl-tRNASec selenium transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020033">
        <property type="entry name" value="PyrdxlP-dep_transferase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018319">
        <property type="entry name" value="SelA-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03841">
        <property type="entry name" value="SelA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03576">
        <property type="entry name" value="pyridox_MJ0158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106722" description="UPF0425 pyridoxal phosphate-dependent protein MJ0158">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="13"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="27"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="44"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="77"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="99"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="113"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="124"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="131"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="142"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="175"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="182"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="194"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="200"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="208"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="216"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="222"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="234"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="256"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="282"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="288"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="296"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="331"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="334"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="342"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="350"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="357"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="16201757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2AEU"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2AEV"/>
        </source>
    </evidence>
    <sequence length="374" mass="41880" checksum="3ED87DC642AA0AB8" modified="1996-11-01" version="1">MLSDYEEFLRLEKARKIILEILNEKGRDALYDLSGLSGGFLIDEKDKALLNTYIGSSYFAEKVNEYGLKHLGGDENDKCVGFNRTSSAILATILALKPKKVIHYLPELPGHPSIERSCKIVNAKYFESDKVGEILNKIDKDTLVIITGSTMDLKVIELENFKKVINTAKNKEAIVFVDDASGARVRLLFNQPPALKLGADLVVTSTDKLMEGPRGGLLAGKKELVDKIYIEGTKFGLEAQPPLLAGIYRALKNFNLERIRKAFERAKNFDLSKIEKLNKELKAIDDNINIVYERTPTGFVIKRVYKDDTINIKKLIEIGFNLLKNYGIITITVAGMPGASKSLRIDLTSRDAERIDDNYIIKAIVESIKMAFKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q57627</accession>
    <name>Y163_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0163</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0163</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64320">
        <property type="entry name" value="D64320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869658.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57627"/>
    <dbReference type="SMR" id="Q57627"/>
    <dbReference type="STRING" id="243232.MJ_0163"/>
    <dbReference type="EnsemblBacteria" id="AAB98151">
        <property type="protein sequence ID" value="AAB98151"/>
        <property type="gene ID" value="MJ_0163"/>
    </dbReference>
    <dbReference type="GeneID" id="1451010"/>
    <dbReference type="KEGG" id="mja:MJ_0163"/>
    <dbReference type="eggNOG" id="arCOG03412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122697_0_0_2"/>
    <dbReference type="OMA" id="IEKNEMP"/>
    <dbReference type="OrthoDB" id="98206at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106725" description="Uncharacterized protein MJ0163">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="187" mass="21510" checksum="DC1FAA5F5CBA3861" modified="1996-11-01" version="1">MDAIIIFLILFIVGVLIGVGVYYYKEKERKKTYKIIEMEIIENLKELKPYVAPDEGREYTKEFDLVEIALSYDIEDIIVVNDEGLVIATTLKDADEVGATASSIFEYIKKLCGNIKKVVIFKEDSYLYIYPLKLYGENLYVIIESKIALDVIEEKEILKRITGVLKKYFSTITTIEQEIPEEALLSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q57640</accession>
    <name>Y181_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0181</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0181</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64322">
        <property type="entry name" value="F64322"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57640"/>
    <dbReference type="STRING" id="243232.MJ_0181"/>
    <dbReference type="PRIDE" id="Q57640"/>
    <dbReference type="EnsemblBacteria" id="AAB98178">
        <property type="protein sequence ID" value="AAB98178"/>
        <property type="gene ID" value="MJ_0181"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0181"/>
    <dbReference type="eggNOG" id="arCOG12715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1127094_0_0_2"/>
    <dbReference type="OrthoDB" id="87610at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106730" description="Uncharacterized protein MJ0181">
        <location>
            <begin position="1"/>
            <end position="246"/>
        </location>
    </feature>
    <sequence length="246" mass="30120" checksum="3EA28EBF3FCE8790" modified="1996-11-01" version="1">MEKIFNISFSMPKSYELKDFIKQYDFFNDDKIAEKLERFFKAINFTNPRHLKKVLNKYAILIEFKNSKIDNERLIPEIIRIENGERKRKGYLFDTVFVLYFIILYEFYYGKYLEVKRYKCRLQTNTGLQSYFERYSLLSQIMKVIKNRNANDMDRVITNLMLLYSQLGYRYNYEIKGRKFYKLVINREIRDKDYNIANELSIELKEAGITVDFWEYIKNNYEDLIEENYPNPYPFTNLFKMVETYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q57652</accession>
    <name>FER4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0199</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0199</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98183" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98183.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64324">
        <property type="entry name" value="H64324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496418.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57652"/>
    <dbReference type="SMR" id="Q57652"/>
    <dbReference type="STRING" id="243232.MJ_0199"/>
    <dbReference type="EnsemblBacteria" id="AAB98183">
        <property type="protein sequence ID" value="AAB98183"/>
        <property type="gene ID" value="MJ_0199"/>
    </dbReference>
    <dbReference type="GeneID" id="1451048"/>
    <dbReference type="KEGG" id="mja:MJ_0199"/>
    <dbReference type="eggNOG" id="arCOG00959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="InParanoid" id="Q57652"/>
    <dbReference type="OMA" id="CLSCHEI"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="PhylomeDB" id="Q57652"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159132" description="Uncharacterized ferredoxin MJ0199">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="62" mass="6650" checksum="C46FA4A11722276A" modified="1997-11-01" version="2">MAVTIDYSLCKGAECAECVNNCPMEVFEIEGDKVVVARPDDCTYCGVCEDVCPTGAVKVEPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q57663</accession>
    <name>SUCC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinate--CoA ligase [ADP-forming] subunit beta</fullName>
            <ecNumber evidence="1">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="1">SCS-beta</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sucC</name>
        <name type="ordered locus">MJ0210</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the succinate/malate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64326">
        <property type="entry name" value="C64326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869705.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57663"/>
    <dbReference type="SMR" id="Q57663"/>
    <dbReference type="STRING" id="243232.MJ_0210"/>
    <dbReference type="EnsemblBacteria" id="AAB98195">
        <property type="protein sequence ID" value="AAB98195"/>
        <property type="gene ID" value="MJ_0210"/>
    </dbReference>
    <dbReference type="GeneID" id="1451059"/>
    <dbReference type="KEGG" id="mja:MJ_0210"/>
    <dbReference type="eggNOG" id="arCOG01337">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037430_0_2_2"/>
    <dbReference type="InParanoid" id="Q57663"/>
    <dbReference type="OMA" id="ITACDEV"/>
    <dbReference type="OrthoDB" id="72275at2157"/>
    <dbReference type="PhylomeDB" id="Q57663"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00558">
        <property type="entry name" value="Succ_CoA_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_synthase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="PTHR11815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000102885" description="Succinate--CoA ligase [ADP-forming] subunit beta">
        <location>
            <begin position="1"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="1">
        <location>
            <begin position="9"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit alpha</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00558"/>
        </source>
    </evidence>
    <sequence length="364" mass="40907" checksum="16937FC54694770C" modified="1997-11-01" version="1">MKLHEYEAKNIFKKYGIPVPESFLVSKEDDLNSINVDKEVVLKAQVLVGGRGKAGGILFASNKEEFIKKAEELFNKEVKGEKVEKILVEEKLPIEKEYYVSIIIDRDAKKPLIIFSTEGGVDIEEVAEKNPEKIIKYHIDVRKPFLPYIARWIVKEAKLPSNEIGKVADVIYKLYKIFKELDATMVEINPLVITKDGNVYAADAVLHLDDDAAFRHNYEEFEEYKNKEKLPFAYVELDGDVAVIGNGAGLTLASMDIINNLGRKPACFLDIGGGADAETVKLALRKVLENKNVKGIFINILGGITRCDEVAKGIVEVLKEHPNVKFAVRMMGTNEEIGRKILEEHGIPYETSMEEAGRKLIEQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57665</accession>
    <name>ALBA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA/RNA-binding protein Alba</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">albA</name>
        <name type="ordered locus">MJ0212</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">Acetylated. Deacetylation by the SIR2-homolog deacetylase may regulate its activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the histone-like Alba family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64326">
        <property type="entry name" value="E64326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869708.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1NH9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-87"/>
    </dbReference>
    <dbReference type="PDBsum" id="1NH9"/>
    <dbReference type="AlphaFoldDB" id="Q57665"/>
    <dbReference type="SMR" id="Q57665"/>
    <dbReference type="STRING" id="243232.MJ_0212"/>
    <dbReference type="EnsemblBacteria" id="AAB98197">
        <property type="protein sequence ID" value="AAB98197"/>
        <property type="gene ID" value="MJ_0212"/>
    </dbReference>
    <dbReference type="GeneID" id="1451062"/>
    <dbReference type="KEGG" id="mja:MJ_0212"/>
    <dbReference type="eggNOG" id="arCOG01753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110989_1_0_2"/>
    <dbReference type="InParanoid" id="Q57665"/>
    <dbReference type="OMA" id="QFNEGAK"/>
    <dbReference type="OrthoDB" id="111461at2157"/>
    <dbReference type="PhylomeDB" id="Q57665"/>
    <dbReference type="EvolutionaryTrace" id="Q57665"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01122">
        <property type="entry name" value="AlbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036882">
        <property type="entry name" value="Alba-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013795">
        <property type="entry name" value="DNA/RNA-bd_Alba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002775">
        <property type="entry name" value="DNA/RNA-bd_Alba-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01918">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028732">
        <property type="entry name" value="Alba"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82704">
        <property type="entry name" value="SSF82704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00285">
        <property type="entry name" value="TIGR00285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0158">Chromosome</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000151698" description="DNA/RNA-binding protein Alba">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-acetyllysine" evidence="1">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="3"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="78"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="1NH9"/>
        </source>
    </evidence>
    <sequence length="87" mass="9675" checksum="50B16E3E9D2DB157" modified="1996-11-01" version="1">MDNVVLIGKKPVMNYVVAVLTQLTSNDEVIIKARGKAINKAVDVAEMIRNRFIKDIKIKKIEIGTDKVKNPDGREVNVSTIEIVLAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q57707</accession>
    <name>Y259_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0259</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0259</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D64332"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869756.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57707"/>
    <dbReference type="STRING" id="243232.MJ_0259"/>
    <dbReference type="EnsemblBacteria" id="AAB98250">
        <property type="protein sequence ID" value="AAB98250"/>
        <property type="gene ID" value="MJ_0259"/>
    </dbReference>
    <dbReference type="GeneID" id="1451113"/>
    <dbReference type="KEGG" id="mja:MJ_0259"/>
    <dbReference type="eggNOG" id="arCOG05028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1340754_0_0_2"/>
    <dbReference type="OMA" id="QFYPHNI"/>
    <dbReference type="OrthoDB" id="90998at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106761" description="Uncharacterized protein MJ0259">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <sequence length="202" mass="22872" checksum="208E43AF60241A7E" modified="1996-11-01" version="1">MKKLLLIIGIISLMTSMSMCLNNNNLNNLDLKKSILVEVNGTPIEIPLRATVGEAKEVKLINTTDREIYNYYHSKILIYIKGDMNISVKEGGVSIVDLVTKLEWFNQFYPHNIVVELNRTNSTVTVKSIFANGKTSITELKVNESEYLMHNNKTMVIEILKTHNTATITKINNTFIIEGNSLKELDNAETRFVIDMFKGSIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57713</accession>
    <name>FER9_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0265</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0265</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 4 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="SMR" id="Q57713"/>
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    <dbReference type="EnsemblBacteria" id="AAB98252">
        <property type="protein sequence ID" value="AAB98252"/>
        <property type="gene ID" value="MJ_0265"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0265"/>
    <dbReference type="eggNOG" id="arCOG01502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043374_3_3_2"/>
    <dbReference type="InParanoid" id="Q57713"/>
    <dbReference type="OMA" id="KACSKRC"/>
    <dbReference type="PhylomeDB" id="Q57713"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
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        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12798">
        <property type="entry name" value="Fer4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159137" description="Uncharacterized ferredoxin MJ0265">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="3"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 3" evidence="2">
        <location>
            <begin position="68"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="166" mass="18229" checksum="9173A1857002D7F6" modified="1998-06-01" version="2">MVMKKIIMTNFNCDNCGDCVKACMEKNKVGRIAIMEKDGKYIPIVCQHCASAPCKEVCPVSAIEHKDGYVYLNEDVCIGCGLCALACPFGAILMEDKAYKCILCNGDEPACVKACSKRCLELVDVNELIFAKRDKSLDLFSKMSLPTQKSDNSLISKITIDAKVKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57720</accession>
    <name>Y272_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0272</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0272</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98257" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98257.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64334">
        <property type="entry name" value="A64334"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496448.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57720"/>
    <dbReference type="SMR" id="Q57720"/>
    <dbReference type="STRING" id="243232.MJ_0272"/>
    <dbReference type="EnsemblBacteria" id="AAB98257">
        <property type="protein sequence ID" value="AAB98257"/>
        <property type="gene ID" value="MJ_0272"/>
    </dbReference>
    <dbReference type="GeneID" id="1451126"/>
    <dbReference type="KEGG" id="mja:MJ_0272"/>
    <dbReference type="eggNOG" id="arCOG01864">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066192_44_1_2"/>
    <dbReference type="InParanoid" id="Q57720"/>
    <dbReference type="OMA" id="YEPSIWL"/>
    <dbReference type="OrthoDB" id="122167at2157"/>
    <dbReference type="PhylomeDB" id="Q57720"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000149789" description="Uncharacterized HTH-type transcriptional regulator MJ0272">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="66" mass="7738" checksum="32667152D3AD810E" modified="1998-07-15" version="2">MKNKLKYYRALHNLTQEDLAKKLGVSRQTIIAIEKGKYDPSLKLAFKIAKFFGVKIEDIFIYEDDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q57730</accession>
    <name>Y282_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0282</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0282</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64335">
        <property type="entry name" value="C64335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57730"/>
    <dbReference type="SMR" id="Q57730"/>
    <dbReference type="STRING" id="243232.MJ_0282"/>
    <dbReference type="EnsemblBacteria" id="AAB98270">
        <property type="protein sequence ID" value="AAB98270"/>
        <property type="gene ID" value="MJ_0282"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0282"/>
    <dbReference type="eggNOG" id="arCOG00518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118461_1_0_2"/>
    <dbReference type="InParanoid" id="Q57730"/>
    <dbReference type="OMA" id="PIGFARP"/>
    <dbReference type="PhylomeDB" id="Q57730"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011576">
        <property type="entry name" value="Pyridox_Oxase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01243">
        <property type="entry name" value="Putative_PNPOx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106771" description="Uncharacterized protein MJ0282">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <sequence length="149" mass="16574" checksum="CD913E94F5813601" modified="1996-11-01" version="1">MKMFHKIYFGDSVVKLTEEMVKSLENEIVFIATASKDGVPNVAAMRAIKVLDAEKGIVLIADNFMNKTLKNILENPKVALTTANCKDMPYQYKGTAEYYKEGEYLKIAEEVDKALKPDLKPKGAVVIKITEIYNLKSGPDAGKLIAKDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57741</accession>
    <name>KTHY_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable thymidylate kinase</fullName>
            <ecNumber>2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tmk</name>
        <name type="ordered locus">MJ0293</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + dTMP = ADP + dTDP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the thymidylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64336">
        <property type="entry name" value="F64336"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57741"/>
    <dbReference type="SMR" id="Q57741"/>
    <dbReference type="STRING" id="243232.MJ_0293"/>
    <dbReference type="EnsemblBacteria" id="AAB98278">
        <property type="protein sequence ID" value="AAB98278"/>
        <property type="gene ID" value="MJ_0293"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0293"/>
    <dbReference type="eggNOG" id="arCOG01891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049131_1_3_2"/>
    <dbReference type="InParanoid" id="Q57741"/>
    <dbReference type="OMA" id="VMTREPG"/>
    <dbReference type="PhylomeDB" id="Q57741"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:thymidylate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009041">
        <property type="term" value="F:uridylate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155386" description="Probable thymidylate kinase">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="188" mass="21687" checksum="A7EC8A9B17F08585" modified="1997-11-01" version="1">MVDNMFIVFEGIDGSGKTTQSKLLAKKMDAFWTYEPSNSLVGKIIREILSGKTEVDNKTLALLFAADRIEHTKLIKEELKKRDVVCDRYLYSSIAYQSVAGVDENFIKSINRYALKPDIVFLLIVDIETALKRVKTKDIFEKKDFLKKVQDKYLELAEEYNFIVIDTTKKSVEEVHNEIIGYLKNIPH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57746</accession>
    <name>Y298_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0298</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0298</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64337">
        <property type="entry name" value="C64337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869796.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57746"/>
    <dbReference type="SMR" id="Q57746"/>
    <dbReference type="STRING" id="243232.MJ_0298"/>
    <dbReference type="EnsemblBacteria" id="AAB98297">
        <property type="protein sequence ID" value="AAB98297"/>
        <property type="gene ID" value="MJ_0298"/>
    </dbReference>
    <dbReference type="GeneID" id="1451153"/>
    <dbReference type="KEGG" id="mja:MJ_0298"/>
    <dbReference type="eggNOG" id="arCOG00519">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105338_0_0_2"/>
    <dbReference type="InParanoid" id="Q57746"/>
    <dbReference type="OMA" id="CRGWDTN"/>
    <dbReference type="OrthoDB" id="95849at2157"/>
    <dbReference type="PhylomeDB" id="Q57746"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026816">
        <property type="entry name" value="Flavodoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12724">
        <property type="entry name" value="Flavodoxin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106781" description="Uncharacterized protein MJ0298">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00088"/>
        </source>
    </evidence>
    <sequence length="150" mass="17228" checksum="F0655E356CBDB828" modified="1996-11-01" version="1">MKALILYKSIHHKNTEKIAKTIADELNADIYNIDKVSPDIIENYDLIGFGSGIYFGKHHKSIFKFLDKISKTNKKAFIFSTAGFPFLKSMFHKELRDKLKSKGFEILGEFCCKGYHTYGIFKLFGGLNKNHPNEDDIKKAKEFAKSILKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57767</accession>
    <name>Y319_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0319</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0319</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64339">
        <property type="entry name" value="H64339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869817.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57767"/>
    <dbReference type="SMR" id="Q57767"/>
    <dbReference type="STRING" id="243232.MJ_0319"/>
    <dbReference type="EnsemblBacteria" id="AAB98304">
        <property type="protein sequence ID" value="AAB98304"/>
        <property type="gene ID" value="MJ_0319"/>
    </dbReference>
    <dbReference type="GeneID" id="1451174"/>
    <dbReference type="KEGG" id="mja:MJ_0319"/>
    <dbReference type="eggNOG" id="arCOG04140">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179754_1_0_2"/>
    <dbReference type="InParanoid" id="Q57767"/>
    <dbReference type="OMA" id="VVHSIDM"/>
    <dbReference type="OrthoDB" id="126791at2157"/>
    <dbReference type="PhylomeDB" id="Q57767"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003831">
        <property type="entry name" value="DUF211"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023129">
        <property type="entry name" value="MTH889-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240">
        <property type="entry name" value="PTHR42240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02680">
        <property type="entry name" value="DUF211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160363">
        <property type="entry name" value="SSF160363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106791" description="Uncharacterized protein MJ0319">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <sequence length="97" mass="10920" checksum="85BB11AC11236FC5" modified="1996-11-01" version="1">MNGIRRIVLDILKPHEPKITDMALKLTSLSNIDGVNITVYEIDKETENVKVTIEGNNLDFDEIQEIIESLGGTIHSIDEVVAGKKIIEEVRTPQDRH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57789</accession>
    <name>Y343_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0343</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0343</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64342">
        <property type="entry name" value="G64342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869841.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57789"/>
    <dbReference type="SMR" id="Q57789"/>
    <dbReference type="STRING" id="243232.MJ_0343"/>
    <dbReference type="PRIDE" id="Q57789"/>
    <dbReference type="EnsemblBacteria" id="AAB98333">
        <property type="protein sequence ID" value="AAB98333"/>
        <property type="gene ID" value="MJ_0343"/>
    </dbReference>
    <dbReference type="GeneID" id="1451199"/>
    <dbReference type="KEGG" id="mja:MJ_0343"/>
    <dbReference type="eggNOG" id="arCOG11380">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_335763_0_0_2"/>
    <dbReference type="OMA" id="IHFMENI"/>
    <dbReference type="OrthoDB" id="132240at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106813" description="Uncharacterized protein MJ0343">
        <location>
            <begin position="1"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="587"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="620"/>
            <end position="640"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="849" mass="94457" checksum="D7103CAD1CAB5C5C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q57847</accession>
    <name>Y404_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0404</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0404</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64350">
        <property type="entry name" value="D64350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869903.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57847"/>
    <dbReference type="STRING" id="243232.MJ_0404"/>
    <dbReference type="EnsemblBacteria" id="AAB98391">
        <property type="protein sequence ID" value="AAB98391"/>
        <property type="gene ID" value="MJ_0404"/>
    </dbReference>
    <dbReference type="GeneID" id="1451264"/>
    <dbReference type="KEGG" id="mja:MJ_0404"/>
    <dbReference type="eggNOG" id="arCOG04903">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1567127_0_0_2"/>
    <dbReference type="InParanoid" id="Q57847"/>
    <dbReference type="OMA" id="GCARTNE"/>
    <dbReference type="OrthoDB" id="94396at2157"/>
    <dbReference type="PhylomeDB" id="Q57847"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012356">
        <property type="entry name" value="UCP018781_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09885">
        <property type="entry name" value="DUF2112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018781">
        <property type="entry name" value="UCP018781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03271">
        <property type="entry name" value="methan_mark_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106855" description="Uncharacterized protein MJ0404">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <sequence length="150" mass="16867" checksum="3D3803E0DAC73296" modified="1996-11-01" version="1">MKKIFIYPPNSLILTDLVERFGHKPLNLNIVIGKLVRNPEIDSPPMNITDEEPKKGLKYAAVEVPSGVRGRMALIGPLIEEAEAAIIMDDAPIAFGCIGCQRTNELTLYLVRRKNIPILRVKYPTNEEEAEILVNKIANFLKSLEENQEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q57906</accession>
    <name>Y481_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0481</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0481</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64360">
        <property type="entry name" value="B64360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869983.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57906"/>
    <dbReference type="EnsemblBacteria" id="AAB98472">
        <property type="protein sequence ID" value="AAB98472"/>
        <property type="gene ID" value="MJ_0481"/>
    </dbReference>
    <dbReference type="GeneID" id="1451344"/>
    <dbReference type="KEGG" id="mja:MJ_0481"/>
    <dbReference type="eggNOG" id="arCOG05031">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340193_0_0_2"/>
    <dbReference type="OMA" id="VGEIGEC"/>
    <dbReference type="OrthoDB" id="104553at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106889" description="Uncharacterized protein MJ0481">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <sequence length="98" mass="11218" checksum="6F31A408240C3CB3" modified="1996-11-01" version="1">MYNCPYCGKDCVNEAAVNIYLKMVEKFFKYQNKGSDITFEKYPTVGEVGECKETGGRIYLCPYCKKPFKAYYEKDKVVITCPNCGETLCLPATNRTFC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q57910</accession>
    <name>Y486_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uncharacterized protein MJ0486</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0486</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64360">
        <property type="entry name" value="F64360"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57910"/>
    <dbReference type="SMR" id="Q57910"/>
    <dbReference type="STRING" id="243232.MJ_0486"/>
    <dbReference type="DNASU" id="1451348"/>
    <dbReference type="EnsemblBacteria" id="AAB98477">
        <property type="protein sequence ID" value="AAB98477"/>
        <property type="gene ID" value="MJ_0486"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0486"/>
    <dbReference type="eggNOG" id="arCOG01362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060920_0_0_2"/>
    <dbReference type="InParanoid" id="Q57910"/>
    <dbReference type="OMA" id="NAGFTCP"/>
    <dbReference type="PhylomeDB" id="Q57910"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005911">
        <property type="entry name" value="YhcC-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF1">
        <property type="entry name" value="PTHR11135:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01091">
        <property type="entry name" value="uncharacterized_CHP01210-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01212">
        <property type="entry name" value="TIGR01212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000106893" description="Uncharacterized protein MJ0486">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="34"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9X0Z6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="322" mass="37390" checksum="F3D074B32180B805" modified="1996-11-01" version="1">MVVIMLNYDDIKIIDEIYSEGYLFAQYGIYIKKKFKQKIFKIPVDIGLGCPHKKNGGCIFCPEMGRPISVKYCSAKIPLKEQIKKQMENQKKKGFKKFYIYFYPGTNTYAPAEKLKEIWDFSLSYKEVIGLSIGTRPDCLEKEKLDILAEYVENGYDIWIDLGVQSMHQKTLEILNRGHDVSDIIKAIKDCHKRGIKVCGHVILGLPGESWKEMMETAKILSLLEIEAVKIYPLVVVKGTKLEEMYWKGEYRTLDENQYISLVCDFLEHLSPYVLIQRLSKDKVPESIKVSPEWYLGRLKIMNKVSEILKKRGTKQGARFFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q57912</accession>
    <name>Y488_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0488</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0488</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64360">
        <property type="entry name" value="H64360"/>
    </dbReference>
    <dbReference type="PDB" id="3WB0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.91 A"/>
        <property type="chains" value="A/B/C/D=3-158"/>
    </dbReference>
    <dbReference type="PDB" id="3WB1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B/C/D=3-158"/>
    </dbReference>
    <dbReference type="PDB" id="3WB2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.44 A"/>
        <property type="chains" value="A/B/C/D=3-158"/>
    </dbReference>
    <dbReference type="PDB" id="5D5Q">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B/C/D=1-158"/>
    </dbReference>
    <dbReference type="PDBsum" id="3WB0"/>
    <dbReference type="PDBsum" id="3WB1"/>
    <dbReference type="PDBsum" id="3WB2"/>
    <dbReference type="PDBsum" id="5D5Q"/>
    <dbReference type="AlphaFoldDB" id="Q57912"/>
    <dbReference type="SMR" id="Q57912"/>
    <dbReference type="STRING" id="243232.MJ_0488"/>
    <dbReference type="EnsemblBacteria" id="AAB98479">
        <property type="protein sequence ID" value="AAB98479"/>
        <property type="gene ID" value="MJ_0488"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0488"/>
    <dbReference type="eggNOG" id="arCOG04863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691544_0_0_2"/>
    <dbReference type="OMA" id="SMLVIMD"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010254">
        <property type="entry name" value="B12-dep_deHydtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012019">
        <property type="entry name" value="HcgB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11576">
        <property type="entry name" value="HcgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005018">
        <property type="entry name" value="UCP005018"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106894" description="Uncharacterized protein MJ0488">
        <location>
            <begin position="1"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="4"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="39"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="65"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="73"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="82"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="123"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="3WB0"/>
        </source>
    </evidence>
    <sequence length="158" mass="17784" checksum="4BB76EAE604F7078" modified="1996-11-01" version="1">MIVMEEIIKKAFIESINNIRRGDKEEELKKIQEKIVNAKKIVVATNNQKKFKVIRDIMLRVCNAEIKMLDIDTRFADLTRMPALTKGLIALDIEKADLYIARGRLGAPGSGSMLVILDEKGRVLTASLSPSSVIHKEDIEERIKKELIEALSRIGISI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q57932</accession>
    <name>Y512_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0512</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0512</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64363">
        <property type="entry name" value="H64363"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57932"/>
    <dbReference type="STRING" id="243232.MJ_0512"/>
    <dbReference type="EnsemblBacteria" id="AAB98511">
        <property type="protein sequence ID" value="AAB98511"/>
        <property type="gene ID" value="MJ_0512"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0512"/>
    <dbReference type="eggNOG" id="arCOG08280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1182896_0_0_2"/>
    <dbReference type="OMA" id="SKRCYIP"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106905" description="Uncharacterized protein MJ0512">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <sequence length="237" mass="27072" checksum="FF08A53188543448" modified="1996-11-01" version="1">MIFMVLGKIKSLLPNSQILTKVKIVDIRRKESEEGSIFYIGTMVDKDGVANFITTIPLERGKCYEIFGRITEEKSVRIVEKVIKGVKYPREIPEIPKEQLYNRGEVLDVKVPAILEVSQSTIFVNYYCKICRGIVDTKIKPRGLVYICRNCGEIDPEDVDVKIKVFGKIHFGTSSKRCYIPPATLEQFMPGILDMLEEYGIDDTIREICLKLNGKTFLVRGFEGKEGNYIITEMEDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q57937</accession>
    <name>Y517_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0517</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0517</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64364">
        <property type="entry name" value="E64364"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57937"/>
    <dbReference type="SMR" id="Q57937"/>
    <dbReference type="STRING" id="243232.MJ_0517"/>
    <dbReference type="EnsemblBacteria" id="AAB98506">
        <property type="protein sequence ID" value="AAB98506"/>
        <property type="gene ID" value="MJ_0517"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0517"/>
    <dbReference type="eggNOG" id="arCOG04835">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_133051_0_0_2"/>
    <dbReference type="OMA" id="IDLGLCW"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3070.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036606">
        <property type="entry name" value="EhaM-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012056">
        <property type="entry name" value="NiFe-hyd_3_EhaM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09218">
        <property type="entry name" value="DUF1959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101332">
        <property type="entry name" value="SSF101332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106907" description="Uncharacterized protein MJ0517">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <sequence length="140" mass="16386" checksum="2FE6B33714CE0486" modified="1996-11-01" version="1">MVIMTEIVDIDKKYVENSLKQKMNVLKDNRFLMDDVFIPIAKALKIEVEEVIEIFAKKLDFASCYELHAYAEQAKMGCLGRKVDIDLGLCWLSDFFGLIKKEEADLIRKKVVESYLLYKKPYKEALEEGRQMIIKLLKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q57978</accession>
    <name>Y558_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0558</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0558</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64369">
        <property type="entry name" value="F64369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870062.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57978"/>
    <dbReference type="SMR" id="Q57978"/>
    <dbReference type="STRING" id="243232.MJ_0558"/>
    <dbReference type="EnsemblBacteria" id="AAB98552">
        <property type="protein sequence ID" value="AAB98552"/>
        <property type="gene ID" value="MJ_0558"/>
    </dbReference>
    <dbReference type="GeneID" id="1451423"/>
    <dbReference type="KEGG" id="mja:MJ_0558"/>
    <dbReference type="eggNOG" id="arCOG04399">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090237_0_0_2"/>
    <dbReference type="InParanoid" id="Q57978"/>
    <dbReference type="OMA" id="PHIKQKE"/>
    <dbReference type="OrthoDB" id="41689at2157"/>
    <dbReference type="PhylomeDB" id="Q57978"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012015">
        <property type="entry name" value="UCP_HTH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004955">
        <property type="entry name" value="HTH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106930" description="Uncharacterized protein MJ0558">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <sequence length="261" mass="29206" checksum="706AD20896269F4F" modified="1996-11-01" version="1">MKKRNITEFQVLSEIIRKQPHIKQKEIAENLGITVQAVSEHIRNLVKEGYVKSRGRGEYVVTEKGLRKLKNWISEFKDYLDEINTAVYRYKDIWPAIADEDVKDGETVYLFMKNGLLYASKQPKGEAKAKALYGGKKGEDIAICEIKGIIDVPKGKVIVFRIPPEVVGGSRAVDFNLIKENIDNLDDYVIATMGTVAYVVACKLGLKPDIRFAVPEAIVNACNRGCNVIALITGKMAEKVIKKLDNAKISYTVLDATKENK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q57985</accession>
    <name>Y565_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0565</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0565</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64370">
        <property type="entry name" value="E64370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870069.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57985"/>
    <dbReference type="STRING" id="243232.MJ_0565"/>
    <dbReference type="EnsemblBacteria" id="AAB98561">
        <property type="protein sequence ID" value="AAB98561"/>
        <property type="gene ID" value="MJ_0565"/>
    </dbReference>
    <dbReference type="GeneID" id="1451430"/>
    <dbReference type="KEGG" id="mja:MJ_0565"/>
    <dbReference type="eggNOG" id="arCOG04938">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143944_0_0_2"/>
    <dbReference type="InParanoid" id="Q57985"/>
    <dbReference type="OMA" id="FEDWADW"/>
    <dbReference type="OrthoDB" id="119579at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106933" description="Uncharacterized protein MJ0565">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="147" mass="15900" checksum="A19D2A29BDFB36AB" modified="1996-11-01" version="1">MDIKNMRNVIVSLSLVFGLLFTVSGIIEIIIGLYSILGFKIELPLFVGDVFGGLALLAVGIAYFLGVKKAVDRDIKAVSYLFTASIIGLGIGVIAFLILISDAIGFLLGFEDWADWGFFNDLTVYLVLGMLAIIPYRIAKIISSSTT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q58009</accession>
    <name>Y589_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0589</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0589</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64373">
        <property type="entry name" value="E64373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870093.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58009"/>
    <dbReference type="STRING" id="243232.MJ_0589"/>
    <dbReference type="EnsemblBacteria" id="AAB98585">
        <property type="protein sequence ID" value="AAB98585"/>
        <property type="gene ID" value="MJ_0589"/>
    </dbReference>
    <dbReference type="GeneID" id="1451454"/>
    <dbReference type="KEGG" id="mja:MJ_0589"/>
    <dbReference type="eggNOG" id="arCOG08294">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2191035_0_0_2"/>
    <dbReference type="OMA" id="RFVENWD"/>
    <dbReference type="OrthoDB" id="106607at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106947" description="Uncharacterized protein MJ0589">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <sequence length="102" mass="12265" checksum="A613FC1184D66358" modified="1996-11-01" version="1">MRPKEVWRELLEIAKNYYDEDKIFYSKTKRGAYRIKSFTKDKIVIEKLRGKLDEVLGKKRFVENWDKLVHGVEWNIPPAIKSFLKLHPKIVENEDGNLIYKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58012</accession>
    <name>BRIX_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable Brix domain-containing ribosomal biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0594</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in the biogenesis of the ribosome.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64374">
        <property type="entry name" value="B64374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870098.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58012"/>
    <dbReference type="SMR" id="Q58012"/>
    <dbReference type="STRING" id="243232.MJ_0594"/>
    <dbReference type="EnsemblBacteria" id="AAB98595">
        <property type="protein sequence ID" value="AAB98595"/>
        <property type="gene ID" value="MJ_0594"/>
    </dbReference>
    <dbReference type="GeneID" id="1451459"/>
    <dbReference type="KEGG" id="mja:MJ_0594"/>
    <dbReference type="eggNOG" id="arCOG03247">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107897_1_0_2"/>
    <dbReference type="InParanoid" id="Q58012"/>
    <dbReference type="OMA" id="FYIEVHK"/>
    <dbReference type="OrthoDB" id="61656at2157"/>
    <dbReference type="PhylomeDB" id="Q58012"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00699">
        <property type="entry name" value="BriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023548">
        <property type="entry name" value="Brix_dom_Rbsml_bgen_prot"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="chain" id="PRO_0000120270" description="Probable Brix domain-containing ribosomal biogenesis protein">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Brix" evidence="1">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00699"/>
        </source>
    </evidence>
    <sequence length="169" mass="20053" checksum="9FF6090865C6E4B0" modified="1996-11-01" version="1">MILTTSRKPSQRTRSFARDLERTLNIPYVQRGKLSLKEIFEIDKHVLLIGEFKANPGTLVVYDVENEKRLSSFISVKLQREICGEKIYNDDGIRIKISRELKDNEEFQKYYEIYDEFLFQHLNINEDSDITLRLEKDPKYLFAIQFYKGRVKIGPLIRIKSIKLFDSLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58053</accession>
    <name>Y636_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0636</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0636</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64379">
        <property type="entry name" value="D64379"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58053"/>
    <dbReference type="SMR" id="Q58053"/>
    <dbReference type="STRING" id="243232.MJ_0636"/>
    <dbReference type="PRIDE" id="Q58053"/>
    <dbReference type="EnsemblBacteria" id="AAB98630">
        <property type="protein sequence ID" value="AAB98630"/>
        <property type="gene ID" value="MJ_0636"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0636"/>
    <dbReference type="eggNOG" id="arCOG01068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718895_0_0_2"/>
    <dbReference type="InParanoid" id="Q58053"/>
    <dbReference type="OMA" id="MNRIYYE"/>
    <dbReference type="PhylomeDB" id="Q58053"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004148">
        <property type="term" value="F:dihydrolipoyl dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106964" description="Uncharacterized protein MJ0636">
        <location>
            <begin position="1"/>
            <end position="397"/>
        </location>
    </feature>
    <sequence length="397" mass="45232" checksum="F97631613501CA3A" modified="1996-11-01" version="1">MLFGESMTLKIAVVGAGPAGRTSAMFLAKNGFDVDLFEKDRVGGTCLNYGCTYITGLREMADIINNLSILKGEKVHLEEIISFKELQEKINKIQDRIRNKLEKETKELGVNIKYKEFKNKHKNDYDYIIYATGRNYPSNYNGYEVLTHKDIPNLRELPENILIIGGGVVATEYASIFSDFGCNVVLYTRSKILKEIKDEEIRDYLMKKVINFKIINDKEELENLLKDESYTKILAIGGNGRFKTDDYLRVLNEEKVYACGDCLINGGGNTPISRMEGRVVAQNIYNEINNKPLIKPNYELIPKTIRLSLTISYVGKQTNNYKTIRSCVGKGNFFKVLSGVGINKIYYEDGKIVGAITMMPCAEILPYFTQLIRGIDVYNNFMEVHPSTDIFYKEFRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58074</accession>
    <name>Y660_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0660</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0660</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAB98653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D64382"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58074"/>
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    <dbReference type="STRING" id="243232.MJ_0660"/>
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        <property type="protein sequence ID" value="AAB98653"/>
        <property type="gene ID" value="MJ_0660"/>
    </dbReference>
    <dbReference type="GeneID" id="8805396"/>
    <dbReference type="KEGG" id="mja:MJ_0660"/>
    <dbReference type="eggNOG" id="arCOG01641">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_182721_0_0_2"/>
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    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106977" description="Uncharacterized protein MJ0660">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00208"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="77" mass="8631" checksum="A8A2ED23F22F68C0" modified="1996-11-01" version="1">MFNNKGRRNVRNNEVRRNVPVKEGETYTVTIEDMGRGGDGIARVEGFVVFVPETQKGETVNVKITAVKSKFAFAEKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q58083</accession>
    <name>H669_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable ATP-dependent RNA helicase MJ0669</fullName>
            <ecNumber>3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0669</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="1465" last="1470">
            <title>Crystal structure of a DEAD box protein from the hyperthermophile Methanococcus jannaschii.</title>
            <authorList>
                <person name="Story R.M."/>
                <person name="Li H."/>
                <person name="Abelson J.N."/>
            </authorList>
            <dbReference type="PubMed" id="11171974"/>
            <dbReference type="DOI" id="10.1073/pnas.98.4.1465"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS)</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64383">
        <property type="entry name" value="E64383"/>
    </dbReference>
    <dbReference type="PDB" id="1HV8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A/B=1-367"/>
    </dbReference>
    <dbReference type="PDBsum" id="1HV8"/>
    <dbReference type="AlphaFoldDB" id="Q58083"/>
    <dbReference type="SMR" id="Q58083"/>
    <dbReference type="STRING" id="243232.MJ_0669"/>
    <dbReference type="REBASE" id="203805">
        <property type="entry name" value="Ppe892ORF686P"/>
    </dbReference>
    <dbReference type="REBASE" id="205577">
        <property type="entry name" value="Ppe194ORF513P"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q58083"/>
    <dbReference type="EnsemblBacteria" id="AAB98663">
        <property type="protein sequence ID" value="AAB98663"/>
        <property type="gene ID" value="MJ_0669"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0669"/>
    <dbReference type="eggNOG" id="arCOG00558">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_3_2"/>
    <dbReference type="InParanoid" id="Q58083"/>
    <dbReference type="OMA" id="EHKDGQR"/>
    <dbReference type="PhylomeDB" id="Q58083"/>
    <dbReference type="EvolutionaryTrace" id="Q58083"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
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        <location>
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        </location>
    </feature>
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            <begin position="38"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="213"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAD box">
        <location>
            <begin position="154"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="4">
        <location>
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            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="31"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="45"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="53"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
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    <feature type="strand" evidence="4">
        <location>
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    </feature>
    <feature type="strand" evidence="4">
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    </feature>
    <feature type="helix" evidence="4">
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    <feature type="strand" evidence="4">
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    <feature type="helix" evidence="4">
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    <feature type="strand" evidence="4">
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    </feature>
    <feature type="strand" evidence="4">
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    <feature type="helix" evidence="4">
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        </location>
    </feature>
    <feature type="turn" evidence="4">
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    <feature type="strand" evidence="4">
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    <feature type="helix" evidence="4">
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    <evidence type="ECO:0000255" key="1">
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    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
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        <source>
            <dbReference type="PDB" id="1HV8"/>
        </source>
    </evidence>
    <sequence length="367" mass="41920" checksum="1B9102E8DF50CB37" modified="1997-11-01" version="1">MEVEYMNFNELNLSDNILNAIRNKGFEKPTDIQMKVIPLFLNDEYNIVAQARTGSGKTASFAIPLIELVNENNGIEAIILTPTRELAIQVADEIESLKGNKNLKIAKIYGGKAIYPQIKALKNANIVVGTPGRILDHINRGTLNLKNVKYFILDEADEMLNMGFIKDVEKILNACNKDKRILLFSATMPREILNLAKKYMGDYSFIKAKINANIEQSYVEVNENERFEALCRLLKNKEFYGLVFCKTKRDTKELASMLRDIGFKAGAIHGDLSQSQREKVIRLFKQKKIRILIATDVMSRGIDVNDLNCVINYHLPQNPESYMHRIGRTGRAGKKGKAISIINRREYKKLRYIERAMKLKIKKLKFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58121</accession>
    <name>Y711_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0711</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0711</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64388">
        <property type="entry name" value="G64388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870217.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58121"/>
    <dbReference type="STRING" id="243232.MJ_0711"/>
    <dbReference type="EnsemblBacteria" id="AAB98714">
        <property type="protein sequence ID" value="AAB98714"/>
        <property type="gene ID" value="MJ_0711"/>
    </dbReference>
    <dbReference type="GeneID" id="1451589"/>
    <dbReference type="KEGG" id="mja:MJ_0711"/>
    <dbReference type="eggNOG" id="arCOG07502">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867711_0_0_2"/>
    <dbReference type="InParanoid" id="Q58121"/>
    <dbReference type="OMA" id="DIIELAC"/>
    <dbReference type="OrthoDB" id="38971at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106999" description="Uncharacterized protein MJ0711">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="322" mass="38631" checksum="D756464175044F27" modified="1996-11-01" version="1">MDSVLSGKIVQILVGYLKENIYSEQMIKLRMKRICSYEEFLPTYSLIERITEESKEIAIKVYEKNIIVEIVKDFKNKDLIELFELKEELFDEALSYLKKYNADKFLESYTLYCFSEYSDPDSFIKENKSILTKLLRNQYEEVPEEYINELLKSKIKYSTKDLIILDWDNGIILDKNEDFWEEVDIIELACIRVLNLRVFDSMLSEAIQYFTRLQWEKLGYFKLKKLSKDLYLQRISYISYFDSIENVLMLYGDRYYAELYERLCKIFYVSEWIKRVEKKMEMISDIYTMTRQHLTEFYGLLLEGTIVALILLEIILALAKIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58132</accession>
    <name>FER7_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0722</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0722</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ferredoxins are iron-sulfur proteins that transfer electrons probably in the CO-dehydrogenase complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64390">
        <property type="entry name" value="B64390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870227.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58132"/>
    <dbReference type="SMR" id="Q58132"/>
    <dbReference type="STRING" id="243232.MJ_0722"/>
    <dbReference type="EnsemblBacteria" id="AAB98718">
        <property type="protein sequence ID" value="AAB98718"/>
        <property type="gene ID" value="MJ_0722"/>
    </dbReference>
    <dbReference type="GeneID" id="1451599"/>
    <dbReference type="KEGG" id="mja:MJ_0722"/>
    <dbReference type="eggNOG" id="arCOG02587">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_5_2"/>
    <dbReference type="InParanoid" id="Q58132"/>
    <dbReference type="OMA" id="CYDVCPK"/>
    <dbReference type="OrthoDB" id="111853at2157"/>
    <dbReference type="PhylomeDB" id="Q58132"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159135" description="Uncharacterized ferredoxin MJ0722">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="3"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="36"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="77" mass="8847" checksum="7514C8E87906A879" modified="1996-11-01" version="1">MAVEIIVDREKCIGCGRCYDVCPKGPLIWTKDENGKYYAYDVEYCHNCKFCAGRCPTNAILIKVVKPKKKDENKNKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58139</accession>
    <name>Y729_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CBS domain-containing protein MJ0729</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0729</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Acta Crystallogr. F" volume="64" first="605" last="609">
            <title>Crystallization and preliminary crystallographic analysis of merohedrally twinned crystals of MJ0729, a CBS-domain protein from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Fernandez-Millan P."/>
                <person name="Kortazar D."/>
                <person name="Lucas M."/>
                <person name="Martinez-Chantar M.L."/>
                <person name="Astigarraga E."/>
                <person name="Fernandez J.A."/>
                <person name="Sabas O."/>
                <person name="Albert A."/>
                <person name="Mato J.M."/>
                <person name="Martinez-Cruz L.A."/>
            </authorList>
            <dbReference type="PubMed" id="18607087"/>
            <dbReference type="DOI" id="10.1107/s1744309108013432"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Biochemistry" volume="48" first="2760" last="2776">
            <title>The CBS domain protein MJ0729 of Methanocaldococcus jannaschii is a thermostable protein with a pH-dependent self-oligomerization.</title>
            <authorList>
                <person name="Martinez-Cruz L.A."/>
                <person name="Encinar J.A."/>
                <person name="Kortazar D."/>
                <person name="Prieto J."/>
                <person name="Gomez J."/>
                <person name="Fernandez-Millan P."/>
                <person name="Lucas M."/>
                <person name="Arribas E.A."/>
                <person name="Fernandez J.A."/>
                <person name="Martinez-Chantar M.L."/>
                <person name="Mato J.M."/>
                <person name="Neira J.L."/>
            </authorList>
            <dbReference type="PubMed" id="19267448"/>
            <dbReference type="DOI" id="10.1021/bi801920r"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="FEBS Lett." volume="584" first="4485" last="4489">
            <title>The CBS domain protein MJ0729 of Methanocaldococcus jannaschii binds DNA.</title>
            <authorList>
                <person name="Aguado-Llera D."/>
                <person name="Oyenarte I."/>
                <person name="Martinez-Cruz L.A."/>
                <person name="Neira J.L."/>
            </authorList>
            <dbReference type="PubMed" id="20934423"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2010.10.006"/>
        </citation>
        <scope>DNA-BINDING</scope>
        <scope>DOMAIN</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2011" name="Protein Eng. Des. Sel." volume="24" first="161" last="169">
            <title>Nucleotide-induced conformational transitions in the CBS domain protein MJ0729 of Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Martinez-Cruz L.A."/>
                <person name="Encinar J.A."/>
                <person name="Sevilla P."/>
                <person name="Oyenarte I."/>
                <person name="Gomez-Garcia I."/>
                <person name="Aguado-Llera D."/>
                <person name="Garcia-Blanco F."/>
                <person name="Gomez J."/>
                <person name="Neira J.L."/>
            </authorList>
            <dbReference type="PubMed" id="20959390"/>
            <dbReference type="DOI" id="10.1093/protein/gzq073"/>
        </citation>
        <scope>DOMAIN</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2 3">Exhibits a pH-dependent oligomerization state: at pH 7, the dominant species is a dimer, where each monomer is a two-CBS domain protein, and at pH 4.5-4.8, the dominant species is a tetramer, with an oblong shape (PubMed:18607087, PubMed:19267448). At pH 2.5, there is formation of intermolecular hydrogen bonds, suggesting the presence of high-molecular weight species (PubMed:19267448). The physiological dimeric species is thermal and chemically very stable (PubMed:19267448).</text>
    </comment>
    <comment type="domain">
        <text evidence="4 5">The CBS domains bind adenosine derivatives (AMP, ATP, NADP and SAM) (PubMed:20959390). The CBS domains can also bind calf-thymus DNA and E-boxes recognized by transcription factors (PubMed:20934423). Binding of the nucleotides induces protein conformational changes (PubMed:20934423, PubMed:20959390).</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64391">
        <property type="entry name" value="A64391"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58139"/>
    <dbReference type="SMR" id="Q58139"/>
    <dbReference type="STRING" id="243232.MJ_0729"/>
    <dbReference type="EnsemblBacteria" id="AAB98725">
        <property type="protein sequence ID" value="AAB98725"/>
        <property type="gene ID" value="MJ_0729"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0729"/>
    <dbReference type="eggNOG" id="arCOG00629">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2044437_0_0_2"/>
    <dbReference type="OMA" id="CKHSKIE"/>
    <dbReference type="PhylomeDB" id="Q58139"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000107005" description="CBS domain-containing protein MJ0729">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="10"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="70"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18607087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="19267448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="20934423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="20959390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="124" mass="14303" checksum="1C0AAF097936B34A" modified="1996-11-01" version="1">MMIMKVKEVMNKDFIKISPNDIGGEVVQTLYKEKKNYAPVIEDGKLVGWITALDLLIGCKHSKIEDLMLLIDEIKILKENDEVTDELIDEIIKNEDIAYPVINDRDEVVGTLSVFDLLKYYKNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58141</accession>
    <name>ISF1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Iron-sulfur flavoprotein MJ0731</fullName>
            <shortName>Isf-1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>MCJ-1</fullName>
            <shortName>Mj1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0731</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="5309" last="5316">
            <title>Site-specific mutational analysis of a novel cysteine motif proposed to ligate the 4Fe-4S cluster in the iron-sulfur flavoprotein of the thermophilic methanoarchaeon Methanosarcina thermophila.</title>
            <authorList>
                <person name="Leartsakulpanich U."/>
                <person name="Antonkine M.L."/>
                <person name="Ferry J.G."/>
            </authorList>
            <dbReference type="PubMed" id="10986231"/>
            <dbReference type="DOI" id="10.1128/JB.182.19.5309-5316.2000"/>
        </citation>
        <scope>PROTEIN FAMILY</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Redox-active protein probably involved in electron transport.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SsuE family. Isf subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64391">
        <property type="entry name" value="C64391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870236.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58141"/>
    <dbReference type="SMR" id="Q58141"/>
    <dbReference type="STRING" id="243232.MJ_0731"/>
    <dbReference type="EnsemblBacteria" id="AAB98727">
        <property type="protein sequence ID" value="AAB98727"/>
        <property type="gene ID" value="MJ_0731"/>
    </dbReference>
    <dbReference type="GeneID" id="1451608"/>
    <dbReference type="KEGG" id="mja:MJ_0731"/>
    <dbReference type="eggNOG" id="arCOG02573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_3_0_2"/>
    <dbReference type="InParanoid" id="Q58141"/>
    <dbReference type="OMA" id="NLGATFW"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="PhylomeDB" id="Q58141"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000160599" description="Iron-sulfur flavoprotein MJ0731">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="198" mass="22220" checksum="FBED0E57A4C77017" modified="1996-11-01" version="1">MKVFGISGSPRLQGTHFAVNYALNYLKEKGAEVRYFSVSRKKINFCLHCDYCIKKKEGCIHKDDMEEVYENLIWADGVIIGTPVYQGNVTGQLKTLMDRCRAILAKNPKVLRGRVGMAIAVGGDRNGGQEIALRTIHDFFIINEMIPVGGGSFGANLGATFWSKDRGKKGVEEDEEGLRVLRKTLNRFYEVLKEKRGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58150</accession>
    <name>RUBR2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable Rubredoxin-2</fullName>
            <shortName>RD 2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0740</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64392">
        <property type="entry name" value="D64392"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58150"/>
    <dbReference type="SMR" id="Q58150"/>
    <dbReference type="STRING" id="243232.MJ_0740"/>
    <dbReference type="EnsemblBacteria" id="AAB98734">
        <property type="protein sequence ID" value="AAB98734"/>
        <property type="gene ID" value="MJ_0740"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0740"/>
    <dbReference type="eggNOG" id="arCOG04391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_1_1_2"/>
    <dbReference type="InParanoid" id="Q58150"/>
    <dbReference type="OMA" id="CLECHYI"/>
    <dbReference type="PhylomeDB" id="Q58150"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043448">
        <property type="term" value="P:alkane catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000135059" description="Probable Rubredoxin-2">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="55" mass="6331" checksum="9A0DF94A976661D7" modified="1996-11-01" version="1">MIEMARYQCMCGWVYDEDKGEPSQNIPPGTKFEDLPDTFRCPQCGLGKNAFRKID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58169</accession>
    <name>Y759_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0759</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0759</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64394">
        <property type="entry name" value="G64394"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58169"/>
    <dbReference type="SMR" id="Q58169"/>
    <dbReference type="STRING" id="243232.MJ_0759"/>
    <dbReference type="EnsemblBacteria" id="AAB98750">
        <property type="protein sequence ID" value="AAB98750"/>
        <property type="gene ID" value="MJ_0759"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0759"/>
    <dbReference type="eggNOG" id="arCOG04884">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177897_0_0_2"/>
    <dbReference type="OMA" id="MRLMPHF"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035350">
        <property type="entry name" value="DUF5400"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17379">
        <property type="entry name" value="DUF5400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107018" description="Uncharacterized protein MJ0759">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="118" mass="12908" checksum="DDC37AA7855ACACE" modified="1996-11-01" version="1">MVIKKGEIMNEIISLVSLSVIFGAMLSGFATFRLTGMRLMPHFASLMIAFILTLASLFISNNIIGYLAIAFQVITPLTVCPTICNILKTQFQNTGIYSAHLALMGMMFILALGNVILF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58179</accession>
    <name>Y769_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0769</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0769</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="A64396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870274.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58179"/>
    <dbReference type="STRING" id="243232.MJ_0769"/>
    <dbReference type="EnsemblBacteria" id="AAB98774">
        <property type="protein sequence ID" value="AAB98774"/>
        <property type="gene ID" value="MJ_0769"/>
    </dbReference>
    <dbReference type="GeneID" id="1451646"/>
    <dbReference type="KEGG" id="mja:MJ_0769"/>
    <dbReference type="eggNOG" id="arCOG03352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101656_0_0_2"/>
    <dbReference type="OMA" id="KSVYEYM"/>
    <dbReference type="OrthoDB" id="116373at2157"/>
    <dbReference type="PhylomeDB" id="Q58179"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeobox-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006120">
        <property type="entry name" value="Resolvase_HTH_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02796">
        <property type="entry name" value="HTH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="SSF46689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107024" description="Uncharacterized protein MJ0769">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <sequence length="169" mass="20167" checksum="C1DEDFB1EF123898" modified="1996-11-01" version="1">MIEIKISKIPRWDEINKIVKLREKDLVLLKLPKSVYEHPKMAYKLEYLKKKGIFIEVENAKRGRKRKVDDETVKKIHELIIEGYSVREIGNILGIGKSTVWDYAKDCIKELKLERFKKLVWEYREYLINKGKYSPSLQVLFLELEATVDYDLEKAKKILEDIIKHVKEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58205</accession>
    <name>Y795_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0795</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0795</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ1506 and MJ1561.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64399">
        <property type="entry name" value="C64399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870303.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58205"/>
    <dbReference type="STRING" id="243232.MJ_0795"/>
    <dbReference type="EnsemblBacteria" id="AAB98798">
        <property type="protein sequence ID" value="AAB98798"/>
        <property type="gene ID" value="MJ_0795"/>
    </dbReference>
    <dbReference type="GeneID" id="1451675"/>
    <dbReference type="KEGG" id="mja:MJ_0795"/>
    <dbReference type="eggNOG" id="arCOG04400">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_540387_0_0_2"/>
    <dbReference type="InParanoid" id="Q58205"/>
    <dbReference type="OMA" id="QYLEPSK"/>
    <dbReference type="OrthoDB" id="17668at2157"/>
    <dbReference type="PhylomeDB" id="Q58205"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107046" description="Uncharacterized protein MJ0795">
        <location>
            <begin position="1"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="504" mass="57147" checksum="A1A659DB7EAEC74B" modified="1996-11-01" version="1">MKRLKNLFIIFIFLFLLSQVSAYITFKNIDYNAQYLEPSKTYDLYITIESDKEINNTVVYIEPTNQISKENIEIIRGKQWIGHLFPYEYGVAHLIIKINPNAPNYDYKITGYCNYTKGNQQYSENRIFTLPVRGKANLVIETTNNILKVGNNQILLLLTNKGTGTAENIKIEFQNSNNLMVLGDNTFTISALGSKTSTYIPLTIFAKKEGVYSINYKISYKNPYNLLELTQKSETIEGDSKTETLTYQNKNIVEETGILTFNVFPNELISINIKNPTITVGKIENLTISIKNNYKDSLFVVQISKYFIGNNQKSIFIKKGETKNLTFQIKVDKEGVTSIPIVIYFDNNQIEKNLTINVVGKADLVLSGIDIESSFNEIKITGDIDNIGTGKAKSVLISIEKTKNIIPKKPYENYFVGTLNPDDYGSFELHCQINGNVNEIPLKISYRDEDNNLITIYKTVKISKEVVSLKTNNNGGINYLVVGIAILFCVGVVYLIYRGFVKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58210</accession>
    <name>Y800_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0800</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0800</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64399">
        <property type="entry name" value="H64399"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58210"/>
    <dbReference type="SMR" id="Q58210"/>
    <dbReference type="STRING" id="243232.MJ_0800"/>
    <dbReference type="PRIDE" id="Q58210"/>
    <dbReference type="EnsemblBacteria" id="AAB98795">
        <property type="protein sequence ID" value="AAB98795"/>
        <property type="gene ID" value="MJ_0800"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0800"/>
    <dbReference type="eggNOG" id="arCOG02679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_666680_0_0_2"/>
    <dbReference type="InParanoid" id="Q58210"/>
    <dbReference type="OMA" id="ATCFRCA"/>
    <dbReference type="PhylomeDB" id="Q58210"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017676">
        <property type="entry name" value="Methan_mark_15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03286">
        <property type="entry name" value="methan_mark_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107051" description="Uncharacterized protein MJ0800">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="415" mass="44842" checksum="36365D04F28D28F3" modified="1997-11-01" version="1">MKLMVKIALLTCGAEWSGVYHEIEKAAQKVGGELIFPEVDLSYIDEVEDRLGFKVGSANLKLMFARAMSIIEGNTDAEAVFIATCFRCAEGALVRNEVRKLIQQNTNLPVVMYSFTERTKASELLTRMEALTTIVERKSLLARKKQEGISLGIDSGSTTTKAVVMIDDEVAGTGWIYTKDVIESAKEAVNNALKEAGISLDQVETIGTTGYGRYTVGEYFKADLIQEELTVNSKGAAYLADKQEGEATVIDIGGMDNKAISLYDAIPDGFTMGGICAGASGRFFEITARRLGVSLQELGELAAKGDWRKIKMNSYCIVFGIQDLVTALAEGAKAEDVAAAAAHSVAEQVFEQQLQEVDVRDPVILVGGSSLLKGLVIAMEEVLGRKIIVPRYSQLIGAVGAALLSSGYRYKKMKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58215</accession>
    <name>Y805_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0805</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0805</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64400">
        <property type="entry name" value="E64400"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58215"/>
    <dbReference type="SMR" id="Q58215"/>
    <dbReference type="STRING" id="243232.MJ_0805"/>
    <dbReference type="EnsemblBacteria" id="AAB98805">
        <property type="protein sequence ID" value="AAB98805"/>
        <property type="gene ID" value="MJ_0805"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0805"/>
    <dbReference type="eggNOG" id="arCOG04875">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113977_0_0_2"/>
    <dbReference type="InParanoid" id="Q58215"/>
    <dbReference type="OMA" id="FIIEHFD"/>
    <dbReference type="PhylomeDB" id="Q58215"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007162">
        <property type="entry name" value="DUF366"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04017">
        <property type="entry name" value="DUF366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006503">
        <property type="entry name" value="UCP006503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107055" description="Uncharacterized protein MJ0805">
        <location>
            <begin position="1"/>
            <end position="221"/>
        </location>
    </feature>
    <sequence length="221" mass="25926" checksum="6067DA093C185725" modified="1998-07-15" version="3">MMYSRKFQILKENIGLYHGDFMDFEVYDTEYMSIIFVKDRLDYTGKEIEPLWAFKTFDIQKDSIVVFRGRMEVTTENMKDLKDIKREKDIKTPIKSEDAINFVVEHFDVIDLKTIYLRQRLLVFIAKEVIESYNIKLKRDGDDLYFEDKKLSVCIACKGTVSAKIHLGINVKSKGAEHVKIIGLEDLGIKDIDKVMREIAINYAKEIDKIERDLRKTLPLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58230</accession>
    <name>Y820_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative uncharacterized protein MJ0820</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0820</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Could be the product of a pseudogene. MJ0821, MJ0820 and MJ0819 respectively represent the N-terminal, central and C-terminal sections of other members of this family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64402">
        <property type="entry name" value="D64402"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58230"/>
    <dbReference type="SMR" id="Q58230"/>
    <dbReference type="STRING" id="243232.MJ_0820"/>
    <dbReference type="EnsemblBacteria" id="AAB98832">
        <property type="protein sequence ID" value="AAB98832"/>
        <property type="gene ID" value="MJ_0820"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0820"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3163119_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="uncertain"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184682" description="Putative uncharacterized protein MJ0820">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="47" mass="5565" checksum="AB1B30CCE652247B" modified="1996-11-01" version="1">MDDFDKETAIKFMDFLAEEILNKKLSEDEKELIYSYVGGKPINRNCY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58247</accession>
    <name>MPTB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Dihydroneopterin 2',3'-cyclic phosphate phosphodiesterase</fullName>
            <shortName>H2N-cP phosphodiesterase</shortName>
            <shortName>H2Neo-cP phosphodiesterase</shortName>
            <ecNumber>3.1.4.56</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>7,8-dihydro-D-neopterin 2',3'-cyclic phosphate phosphodiesterase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mptB</name>
        <name type="ordered locus">MJ0837</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Biochemistry" volume="48" first="9384" last="9392">
            <title>An Fe2+-dependent cyclic phosphodiesterase catalyzes the hydrolysis of 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate in methanopterin biosynthesis.</title>
            <authorList>
                <person name="Mashhadi Z."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="19746965"/>
            <dbReference type="DOI" id="10.1021/bi9010336"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>GENE NAME</scope>
        <scope>PATHWAY</scope>
        <scope>PH DEPENDENCE</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF HIS-61; HIS-96 AND ASP-167</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Cyclic phosphodiesterase that hydrolyzes the cyclic phosphate of 7,8-dihydroneopterin 2',3'-cyclic phosphate (H2N-cP) and converts it to a mixture of 7,8-dihydroneopterin 2'-phosphate (H2N-2'P) and 7,8-dihydroneopterin 3'-phosphate (H2N-3'P). Is also able to utilize other phosphodiesters as substrates in vitro: hydrolysis of bis-pNPP and pNPPC produces nitrophenyl phosphate, and that of 2',3'-cAMP produces 3'-AMP. ATP, 3',5'-cAMP, GTP, 3',5'-cGMP, and 4',5'-cFMN cannot serve as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7,8-dihydroneopterin 2',3'-cyclic phosphate + H2O = 7,8-dihydroneopterin 3'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58762"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="EC" id="3.1.4.56"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7,8-dihydroneopterin 2',3'-cyclic phosphate + H2O = 7,8-dihydroneopterin 2'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35803"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="ChEBI" id="CHEBI:72777"/>
            <dbReference type="EC" id="3.1.4.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Binds 1 Fe(2+) ion per subunit. Since MptB requires Fe2(+) for activity, the Fe(2+) ion likely has a catalytic role. The enzyme is also active under high concentrations of Mn(2+) in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 Zn(2+) ion per subunit. The Zn(2+) ion is proposed to have a structural role.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 7.5 with bis-pNPP as substrate.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homododecamer.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.56"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64404">
        <property type="entry name" value="E64404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870351.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58247"/>
    <dbReference type="STRING" id="243232.MJ_0837"/>
    <dbReference type="EnsemblBacteria" id="AAB98842">
        <property type="protein sequence ID" value="AAB98842"/>
        <property type="gene ID" value="MJ_0837"/>
    </dbReference>
    <dbReference type="GeneID" id="1451723"/>
    <dbReference type="KEGG" id="mja:MJ_0837"/>
    <dbReference type="eggNOG" id="arCOG01861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109506_0_0_2"/>
    <dbReference type="OMA" id="IRICQAR"/>
    <dbReference type="OrthoDB" id="56504at2157"/>
    <dbReference type="PhylomeDB" id="Q58247"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-16557"/>
    <dbReference type="BRENDA" id="3.1.4.56">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044682">
        <property type="term" value="F:archaeal-specific GTP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000107074" description="Dihydroneopterin 2',3'-cyclic phosphate phosphodiesterase">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="58"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Large reduction in catalytic activity." evidence="2">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Slight reduction in catalytic activity with H2N-cP and 2',3'-cAMP as substrates, but 2-fold increase in activity with bis-pNPP." evidence="2">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Large reduction in catalytic activity." evidence="2">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19746965"/>
        </source>
    </evidence>
    <sequence length="249" mass="28523" checksum="E8A59C478718E613" modified="1996-11-01" version="1">MERLIKLAEQIKDEELRKKVIEFLKNPKATHPGIVDTGISVEEAPASINWHHRYEGGLIEHTISVTKIALKMADVLEEVYGVEVNRDYIIAGALLHDIMKPYNYIRKEDGTFDHYDMFNLDHLTLAVAELYKRDFPIEVIKIVASHHGDHSPTRPNSIEAYIVHYADEADSKINDVAVRVCQARSRDLGISEQEIYKAVNPLKVYEVRSREGKLKTIEFLKDILKSIGIISEDEKKDENNESLENKESN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q58255</accession>
    <name>MCRG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase I subunit gamma</fullName>
            <shortName>MCR I gamma</shortName>
            <ecNumber evidence="1">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme-B sulfoethylthiotransferase gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mcrG</name>
        <name type="ordered locus">MJ0845</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="1">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-coenzyme M reductase gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64405">
        <property type="entry name" value="E64405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870359.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58255"/>
    <dbReference type="SMR" id="Q58255"/>
    <dbReference type="STRING" id="243232.MJ_0845"/>
    <dbReference type="PRIDE" id="Q58255"/>
    <dbReference type="EnsemblBacteria" id="AAB98850">
        <property type="protein sequence ID" value="AAB98850"/>
        <property type="gene ID" value="MJ_0845"/>
    </dbReference>
    <dbReference type="GeneID" id="1451733"/>
    <dbReference type="KEGG" id="mja:MJ_0845"/>
    <dbReference type="eggNOG" id="arCOG04858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1092436_0_0_2"/>
    <dbReference type="InParanoid" id="Q58255"/>
    <dbReference type="OMA" id="IMGHRQP"/>
    <dbReference type="OrthoDB" id="51032at2157"/>
    <dbReference type="PhylomeDB" id="Q58255"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00539">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003178">
        <property type="entry name" value="Me_CoM_Rdtase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036994">
        <property type="entry name" value="Me_CoM_Rdtase_gsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02240">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000264">
        <property type="entry name" value="Meth_CoM_rd_gama"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03259">
        <property type="entry name" value="met_CoM_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147476" description="Methyl-coenzyme M reductase I subunit gamma">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P11562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="260" mass="30174" checksum="34413840D8EADACE" modified="1996-11-01" version="1">MAYKPQFYPGQTKIAQNRRDHMNPDVQLEKLRDIPDDDVVKIMGHRQPGEDYKTVHPPLEEMDLPEDYVRDLVEPLNGAKEGHRIRYIQFTDSMYFAPAQPYDRARTYMWRFRGVDTGTLSGRQVIEMRESDLEALSKNFLIDTAFFDPARIGIRGATVHGHSLRLDENGLMFDALQRYVYDEKTGHVLYVKDQVGRPLDEPVDVGEPLPEEKLKEITTIYRIDGVPMREDEELLTVVKRIHRARTLGGFLPVEDVFEKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58286</accession>
    <name>Y876_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ABC transporter permease protein MJ0876</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0876</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Probably part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64409">
        <property type="entry name" value="D64409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870391.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58286"/>
    <dbReference type="SMR" id="Q58286"/>
    <dbReference type="STRING" id="243232.MJ_0876"/>
    <dbReference type="EnsemblBacteria" id="AAB98879">
        <property type="protein sequence ID" value="AAB98879"/>
        <property type="gene ID" value="MJ_0876"/>
    </dbReference>
    <dbReference type="GeneID" id="1451765"/>
    <dbReference type="KEGG" id="mja:MJ_0876"/>
    <dbReference type="eggNOG" id="arCOG01007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_0_2"/>
    <dbReference type="InParanoid" id="Q58286"/>
    <dbReference type="OMA" id="LLIRNPM"/>
    <dbReference type="OrthoDB" id="36067at2157"/>
    <dbReference type="PhylomeDB" id="Q58286"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060306" description="Putative ABC transporter permease protein MJ0876">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="322"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="351" mass="38580" checksum="764EA88C29AD4F16" modified="1996-11-01" version="1">MNKVGILLILFILSLILPFTALYLAGDTHLITVKDIINFLLKGTTGNEFKDIIIKDVRLPPIIGAVLIGLTISVAGLMLQTLFRNLLASPYTTGISSGVLMVVALVIFIDSLSHLFEIFGEKSILVAGWCGGIFSMILLIIIALRVREANGVIIVALLLSYFFMGLRAYLIANAEELKIQEYWGFTIGSLSKITLGDVIPMTICSIIFIIGVMFLIKSLNALLFGEQYAKSFGLDIKKTRLLVLFFASFITGAIIPYVGLIAFIGIIAPYLARPLIKTSDHRYLVPATMFLGVILMVSCHILSLKYYLPIHYLYGINRPASPLPIGAVLDILGGMLVVYLVYKGEKKIKID</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58287</accession>
    <name>Y877_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ABC transporter permease protein MJ0877</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0877</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Probably part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64409">
        <property type="entry name" value="E64409"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58287"/>
    <dbReference type="SMR" id="Q58287"/>
    <dbReference type="InParanoid" id="Q58287"/>
    <dbReference type="OMA" id="HIIRMAI"/>
    <dbReference type="PhylomeDB" id="Q58287"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060307" description="Putative ABC transporter permease protein MJ0877">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="123" mass="13107" checksum="BE821011FE767D86" modified="1996-11-01" version="1">MDANLLGEKYAISVGVDIKSLRMWLIILSCVLTATVVAFTGPIAFVGITCPILARMICGTSKHIYVIPVTMLLGAVFLVVADILTRPGVLISSTNVLPLLCPLSIIGAPIAIIIYLKIRKMGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58329</accession>
    <name>Y919_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0919</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0919</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64414">
        <property type="entry name" value="G64414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870433.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58329"/>
    <dbReference type="SMR" id="Q58329"/>
    <dbReference type="STRING" id="243232.MJ_0919"/>
    <dbReference type="EnsemblBacteria" id="AAB98927">
        <property type="protein sequence ID" value="AAB98927"/>
        <property type="gene ID" value="MJ_0919"/>
    </dbReference>
    <dbReference type="GeneID" id="1451808"/>
    <dbReference type="KEGG" id="mja:MJ_0919"/>
    <dbReference type="eggNOG" id="arCOG02263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_157714_0_0_2"/>
    <dbReference type="OMA" id="IIRKMRN"/>
    <dbReference type="OrthoDB" id="122071at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090529">
        <property type="term" value="P:cell septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107104" description="Uncharacterized protein MJ0919">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="149" mass="16953" checksum="07B1A6483F957AE4" modified="1996-11-01" version="1">MLEKLKKLLSKKGDNFSTPAPVSVDDYLEEIEEIPLTPVEEEKVIIKVCSIEDEKDAVNAIVMAEAGYIVIAKTPNLEKEIDDEFIEIIRKMRNEVAKFGGMLLALGDEHLLITPRNVVIEKLIKEKKEESNVTKENIEIKEEKEENSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58349</accession>
    <name>Y939_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0939</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0939</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64417">
        <property type="entry name" value="C64417"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58349"/>
    <dbReference type="SMR" id="Q58349"/>
    <dbReference type="STRING" id="243232.MJ_0939"/>
    <dbReference type="EnsemblBacteria" id="AAB98946">
        <property type="protein sequence ID" value="AAB98946"/>
        <property type="gene ID" value="MJ_0939"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0939"/>
    <dbReference type="eggNOG" id="arCOG05059">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1021630_0_0_2"/>
    <dbReference type="OMA" id="FMVKWNG"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107113" description="Uncharacterized protein MJ0939">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <sequence length="276" mass="33454" checksum="97BD69D392BC8FDF" modified="1996-11-01" version="1">MMPMDWEITFKGITYECINCAYCCSCKGWRIYLNYFDRLKLKDYEYAIEPCEGEFKYRLKVNEKGCVLLNNNLCRIHLEKGYEFKPLMCMIFPFSCMIKWDGTPLLIIKHYCSGIKKGKISKKVVNEAIELIKELYFDMFEEIIENGMEHSSKTEIFENFRVDWEEREDFGRYIFSSKTFDEMFERCREIFGNKINKLNLEEIDEIKNNLQRYNTKENEEEILRYLLELNRREHFRKLPFYREVNKLINIGNYLTKYKNVFKGEGEVDKKLFLNLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-09" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58360</accession>
    <name>RFRNP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-free ribonuclease P</fullName>
            <shortName evidence="1">RNA-free RNase P</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Protein-only RNase P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0950</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">RNA-free RNase P that catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HARP family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64418">
        <property type="entry name" value="F64418"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58360"/>
    <dbReference type="SMR" id="Q58360"/>
    <dbReference type="STRING" id="243232.MJ_0950"/>
    <dbReference type="EnsemblBacteria" id="AAB98951">
        <property type="protein sequence ID" value="AAB98951"/>
        <property type="gene ID" value="MJ_0950"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0950"/>
    <dbReference type="eggNOG" id="arCOG00720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109672_0_0_2"/>
    <dbReference type="InParanoid" id="Q58360"/>
    <dbReference type="OMA" id="YIIKKPP"/>
    <dbReference type="PhylomeDB" id="Q58360"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01078">
        <property type="entry name" value="RNA_free_RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014856">
        <property type="entry name" value="RNA_free_RNase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41173">
        <property type="entry name" value="PTHR41173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08745">
        <property type="entry name" value="PIN_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03875">
        <property type="entry name" value="RNA_lig_partner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000136079" description="RNA-free ribonuclease P">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01078"/>
        </source>
    </evidence>
    <sequence length="233" mass="26940" checksum="98B903D6B9523613" modified="1996-11-01" version="1">MGRGMQKQRFCLDTSAFTEPSVRKALGVKTVTELTDKVMDLIAEARIKLNISCHIPYPTVYNELMGFLENENCPRDVIVKVDTWLVKKTPNRYEIKIPSEIFYEYVKDLRERINKGMRIGEEHIIKATDMVYELSKKHPEMGKNEIINKVLSKTINTFRNKYRSALRVGTLDSAPDLDVLLLAKELDAAVVASDGGIEKWAQRLGLRFVDASDFPFMLEEYLKHNDRHLRIKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58368</accession>
    <name>Y958_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0958</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0958</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64419">
        <property type="entry name" value="F64419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870472.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58368"/>
    <dbReference type="STRING" id="243232.MJ_0958"/>
    <dbReference type="EnsemblBacteria" id="AAB98971">
        <property type="protein sequence ID" value="AAB98971"/>
        <property type="gene ID" value="MJ_0958"/>
    </dbReference>
    <dbReference type="GeneID" id="1451856"/>
    <dbReference type="KEGG" id="mja:MJ_0958"/>
    <dbReference type="eggNOG" id="arCOG09676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_651545_0_0_2"/>
    <dbReference type="OMA" id="YENISWE"/>
    <dbReference type="OrthoDB" id="32116at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014006" description="Uncharacterized protein MJ0958">
        <location>
            <begin position="24"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="426" mass="50695" checksum="25317BE81E203A99" modified="1996-11-01" version="1" precursor="true">MKKFILFLIILLFSIYFLNVSSAEVCPFKDGFIIIYHDIGYDKLFGYTYNDSEILYFNNKNLMDITPISDYYFPELYHNITYFECGSTNNLTILSFGYFYFPKNGDGIVFLGVLVYTTKNNKINYTEKILWADVFYSVDDFDISPPACSPNEALLVYCYKMKADYPENILVLINNTNITELKKFEDDDYYTTFQHYIYQPIFIFTTYDSKAKKFYILDGRLSNTSFPLYSYYGGKIHFEDNITLPKYENISWECMDFYSINGTLYIVMKKLNCSNINYAGWYEDYLNQKPYLLIWKNKTIKIISNCSNPYYFVKIKGGEIPIEKSYLKKIFGNKYESIRITDLAYNNGILLIETNENHKLHYYIVKNNSIEEIKLKNIIKLYKKKHSLWDDIKKELEPKIYWVIHNWYIIVLIIAGLLWMAILWKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58384</accession>
    <name>VAPC2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease VapC2</fullName>
            <shortName evidence="1">RNase VapC2</shortName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Putative toxin VapC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">vapC2</name>
        <name type="ordered locus">MJ0974</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB2.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64421">
        <property type="entry name" value="F64421"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58384"/>
    <dbReference type="SMR" id="Q58384"/>
    <dbReference type="STRING" id="243232.MJ_0974"/>
    <dbReference type="EnsemblBacteria" id="AAB98979">
        <property type="protein sequence ID" value="AAB98979"/>
        <property type="gene ID" value="MJ_0974"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0974"/>
    <dbReference type="eggNOG" id="arCOG00710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134210_1_1_2"/>
    <dbReference type="InParanoid" id="Q58384"/>
    <dbReference type="OMA" id="KFRFFID"/>
    <dbReference type="PhylomeDB" id="Q58384"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107125" description="Ribonuclease VapC2">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="11"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <sequence length="149" mass="17733" checksum="58C7AF9A12FC27C2" modified="1997-11-01" version="1">MRRLKMEENKIFFDSNILIYHLCGKVEAKKLIEKVENKEICGFINPIVISEVLFFYIRATTNKRHYDIKKHPEILKSLDLDIVFELFSIFQILDLNSEIVKISREIIKKYCLLPNDALICSTCKFYKINKICSFDDDFKRVDFLEIIEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58388</accession>
    <name>Y978_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0978</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0978</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1086 N-terminal region.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64422">
        <property type="entry name" value="B64422"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58388"/>
    <dbReference type="SMR" id="Q58388"/>
    <dbReference type="STRING" id="243232.MJ_0978"/>
    <dbReference type="PRIDE" id="Q58388"/>
    <dbReference type="EnsemblBacteria" id="AAB98993">
        <property type="protein sequence ID" value="AAB98993"/>
        <property type="gene ID" value="MJ_0978"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0978"/>
    <dbReference type="eggNOG" id="arCOG05125">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086561_0_0_2"/>
    <dbReference type="InParanoid" id="Q58388"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020209">
        <property type="entry name" value="Cas6b_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17262">
        <property type="entry name" value="Cas6b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107127" description="Uncharacterized protein MJ0978">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="147" mass="17112" checksum="28A5962011ED2FCB" modified="1996-11-01" version="1">MGEIILNIRELNLKGEVYRVVNGYAKVKFEEFGVAENMIKYKFISPWIALNEKNYLEYKELDEDGKKELLEKILVGNILSMSKYLDYTVEEKLKAGLLEYEDFVVKYKGNKFIGFWGEFLVNFNIPNYLGIGRKVSKGFGSVIRVEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58432</accession>
    <name>THIC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="1">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="1">HMP-P synthase</shortName>
            <shortName evidence="1">HMP-phosphate synthase</shortName>
            <shortName evidence="1">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiC</name>
        <name type="ordered locus">MJ1026</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 5'-deoxyadenosine + CO + formate + 3 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiC family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99030" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99030.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64428">
        <property type="entry name" value="A64428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870539.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58432"/>
    <dbReference type="SMR" id="Q58432"/>
    <dbReference type="STRING" id="243232.MJ_1026"/>
    <dbReference type="EnsemblBacteria" id="AAB99030">
        <property type="protein sequence ID" value="AAB99030"/>
        <property type="gene ID" value="MJ_1026"/>
    </dbReference>
    <dbReference type="GeneID" id="1451923"/>
    <dbReference type="KEGG" id="mja:MJ_1026"/>
    <dbReference type="eggNOG" id="arCOG02741">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013181_2_2_2"/>
    <dbReference type="InParanoid" id="Q58432"/>
    <dbReference type="OMA" id="TWELFRD"/>
    <dbReference type="OrthoDB" id="25235at2157"/>
    <dbReference type="PhylomeDB" id="Q58432"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="PTHR30557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000152862" description="Phosphomethylpyrimidine synthase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="225"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="426" mass="47477" checksum="7B17477FA327E8EB" modified="2002-11-01" version="2">MTQMDDAKNGIITEEMKIVAEKEKIDIEKLRKLIAKGYVVILKNVNRDTNPVGIGQSLRTKVNANIGTSPDCVDIELEIKKAKIAEKYGADAVMDLSTGGNLEEIRKAIMDAVKIPIGTVPIYEVGKLAREKYGRVIDMNEDLMFKVIEKQAKEGVDFMTLHCGITKQSVERLKRSGRIMGVVSRGGAFLTAYILYHNEENPLYKNFDYLLDILKEHDVTISLGDGMRPGCLADNTDRAQIEELITLGELVERCREKGVQCMVEGPGHIPINYIETNIRLQKSLCKNAPFYVLGPIVTDIAPGYDHITAAIGGALAGYYGADFLCYVTPSEHLRLPTIEDVKEGVIATKIAAQAADVAKGNKLAWEKETEMAYARKNHDWEKQFELAIDKEKARKMREEIPSKEEKACSICGDYCALLMVEELGKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58461</accession>
    <name>Y1061_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized membrane protein MJ1061</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1061</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64432">
        <property type="entry name" value="D64432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870574.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58461"/>
    <dbReference type="SMR" id="Q58461"/>
    <dbReference type="STRING" id="243232.MJ_1061"/>
    <dbReference type="EnsemblBacteria" id="AAB99064">
        <property type="protein sequence ID" value="AAB99064"/>
        <property type="gene ID" value="MJ_1061"/>
    </dbReference>
    <dbReference type="GeneID" id="1451958"/>
    <dbReference type="KEGG" id="mja:MJ_1061"/>
    <dbReference type="eggNOG" id="arCOG01375">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013560_4_1_2"/>
    <dbReference type="InParanoid" id="Q58461"/>
    <dbReference type="OMA" id="HEVMIPK"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="PhylomeDB" id="Q58461"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd05237">
        <property type="entry name" value="UDP_invert_4-6DH_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003869">
        <property type="entry name" value="Polysac_CapD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02719">
        <property type="entry name" value="Polysacc_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000166459" description="Uncharacterized membrane protein MJ1061">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="333" mass="37846" checksum="DA8F3AB5129344B6" modified="1996-11-01" version="1">MFQDISNFYKDKTILVTGGTGSIGKEIVKTLLKFNPKTIRVLDINETALFELEHELNSEKIRCFIGDVRDKDRLKRAIEEVDVVFHAAALKHVPLCEYNPFEAVKTNVIGTQNLIEVAMDEEVEKFITISTDKAVNPVNVMGATKLLAERLTISANLYKGKRKTAFSVVRFGNVLNSRGSILPLLKEQIKKGGPVTLTHPDMTRFIMSINEAVKLVLKACYLAKGGEIFILKMPSVRIKDLIEVVIEELAPKYGYKPEDIEIKIIGKRPGEKLYEELIIEEEIYNLEELEDMFVVYPYGVDGNKNNKIIYNSKDAKFLNKEKIKKILKEISYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58464</accession>
    <name>Y1064_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized acetyltransferase MJ1064</fullName>
            <ecNumber>2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1064</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64432">
        <property type="entry name" value="G64432"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58464"/>
    <dbReference type="SMR" id="Q58464"/>
    <dbReference type="STRING" id="243232.MJ_1064"/>
    <dbReference type="EnsemblBacteria" id="AAB99067">
        <property type="protein sequence ID" value="AAB99067"/>
        <property type="gene ID" value="MJ_1064"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1064"/>
    <dbReference type="eggNOG" id="arCOG01848">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051638_7_0_2"/>
    <dbReference type="InParanoid" id="Q58464"/>
    <dbReference type="PhylomeDB" id="Q58464"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14602">
        <property type="entry name" value="Hexapep_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000068760" description="Uncharacterized acetyltransferase MJ1064">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="214" mass="23402" checksum="908D40935BC72E76" modified="1996-11-01" version="1">MSMIKLLKNTLKDPKKIMRALEFAPSFVFGKIYLSIFGINPLKVHNFGKIHIRKYDSSTIIIKSGILLRDVEIAARGNGKIIIGENFHCEPYVRLNVFEEGILEIGDNCGIGSFSIINATKKITIGSNVLISSHVHIIDGDHGFKKGELIRNQKMVSEPIEIGDDVWIGTGVKILKGVKIGEGAVIGAGSVVTRDIPPYSVAVGVPARVIKKRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58516</accession>
    <name>ASNH1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative asparagine synthetase [glutamine-hydrolyzing] 1</fullName>
            <ecNumber>6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1116</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the asparagine synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99117" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.5.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99117.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64439">
        <property type="entry name" value="C64439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496729.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58516"/>
    <dbReference type="SMR" id="Q58516"/>
    <dbReference type="STRING" id="243232.MJ_1116"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="PRIDE" id="Q58516"/>
    <dbReference type="DNASU" id="1452012"/>
    <dbReference type="EnsemblBacteria" id="AAB99117">
        <property type="protein sequence ID" value="AAB99117"/>
        <property type="gene ID" value="MJ_1116"/>
    </dbReference>
    <dbReference type="GeneID" id="1452012"/>
    <dbReference type="KEGG" id="mja:MJ_1116"/>
    <dbReference type="eggNOG" id="arCOG00071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014658_4_0_2"/>
    <dbReference type="InParanoid" id="Q58516"/>
    <dbReference type="OMA" id="DWSGIYS"/>
    <dbReference type="OrthoDB" id="12911at2157"/>
    <dbReference type="PhylomeDB" id="Q58516"/>
    <dbReference type="UniPathway" id="UPA00134">
        <property type="reaction ID" value="UER00195"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070981">
        <property type="term" value="P:L-asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000056937" description="Putative asparagine synthetase [glutamine-hydrolyzing] 1">
        <location>
            <begin position="2"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="68"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="363"/>
            <end position="364"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="541" mass="62655" checksum="9B3FA64E39A957BB" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58573</accession>
    <name>RELE4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative toxin RelE4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">relE4</name>
        <name type="ordered locus">MJ1173</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. Its cognate antitoxin is RelB4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelE toxin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64446">
        <property type="entry name" value="D64446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870686.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58573"/>
    <dbReference type="SMR" id="Q58573"/>
    <dbReference type="STRING" id="243232.MJ_1173"/>
    <dbReference type="EnsemblBacteria" id="AAB99176">
        <property type="protein sequence ID" value="AAB99176"/>
        <property type="gene ID" value="MJ_1173"/>
    </dbReference>
    <dbReference type="GeneID" id="1452071"/>
    <dbReference type="KEGG" id="mja:MJ_1173"/>
    <dbReference type="eggNOG" id="arCOG02414">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155761_4_0_2"/>
    <dbReference type="InParanoid" id="Q58573"/>
    <dbReference type="OMA" id="HIDSHFV"/>
    <dbReference type="OrthoDB" id="113687at2157"/>
    <dbReference type="PhylomeDB" id="Q58573"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007712">
        <property type="entry name" value="RelE/ParE_toxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035093">
        <property type="entry name" value="RelE/ParE_toxin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004386">
        <property type="entry name" value="Toxin_YafQ-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05016">
        <property type="entry name" value="ParE_toxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143011">
        <property type="entry name" value="SSF143011"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02385">
        <property type="entry name" value="RelE_StbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00053">
        <property type="entry name" value="TIGR00053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107200" description="Putative toxin RelE4">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="94" mass="11450" checksum="85FDCBCD4A8E4A05" modified="1996-11-01" version="1">MYEIEIMPSLDKILQKLSKRDKKKLKAILKKMEEITQNPHHYKNLRHPLNDFKRVHIDKSFVLVFTVDENNKTVIFVDFDHHDNIYKKKKLFKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58577</accession>
    <name>Y1178_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized glycosyltransferase MJ1178</fullName>
            <ecNumber>2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1178</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64446">
        <property type="entry name" value="H64446"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58577"/>
    <dbReference type="SMR" id="Q58577"/>
    <dbReference type="STRING" id="243232.MJ_1178"/>
    <dbReference type="CAZy" id="GT4">
        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99181">
        <property type="protein sequence ID" value="AAB99181"/>
        <property type="gene ID" value="MJ_1178"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1178"/>
    <dbReference type="eggNOG" id="arCOG01403">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009583_2_5_2"/>
    <dbReference type="InParanoid" id="Q58577"/>
    <dbReference type="OMA" id="APVPWFP"/>
    <dbReference type="PhylomeDB" id="Q58577"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000080323" description="Uncharacterized glycosyltransferase MJ1178">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="351" mass="39863" checksum="49998FDC44523FFA" modified="1996-11-01" version="1">MIIMKVLMPSIYYPYIGGITLHVENLVKRLKDIEFHILTYDSYEENEYKNVIIHNVPHLKKFRGISYLINAYKIGKNIIESEGIDLIHSHYAFPQGCVGALLKNKLSIPHILTLHGSDALILKNSIKGRYFFKYATTNSDKIICVSKYIKNQLDENLKNRAIVIYNGVNKEILYNEGDYNFGLFVGAFVPQKGVDILIDAIKDIDFNFKLIGDGKLYKKIENFVVKNNLSHIELLGRKSFDEVASFMRKCSFLVVPSRSEGFGMVAVEGMACSKPVIATRVGGLGEIVIDGYNGLLAEKNNPNDLKEKILELINNEELRKTLGENGKEFSKKFSWEKCVMGVRKVYEELSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58598</accession>
    <name>Y1198_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1198</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1198</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64449">
        <property type="entry name" value="E64449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870710.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2K52">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=131-202"/>
    </dbReference>
    <dbReference type="PDBsum" id="2K52"/>
    <dbReference type="AlphaFoldDB" id="Q58598"/>
    <dbReference type="BMRB" id="Q58598"/>
    <dbReference type="SMR" id="Q58598"/>
    <dbReference type="STRING" id="243232.MJ_1198"/>
    <dbReference type="EnsemblBacteria" id="AAB99202">
        <property type="protein sequence ID" value="AAB99202"/>
        <property type="gene ID" value="MJ_1198"/>
    </dbReference>
    <dbReference type="GeneID" id="1452093"/>
    <dbReference type="KEGG" id="mja:MJ_1198"/>
    <dbReference type="eggNOG" id="arCOG00429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018957_0_0_2"/>
    <dbReference type="InParanoid" id="Q58598"/>
    <dbReference type="OMA" id="IGYGPDF"/>
    <dbReference type="OrthoDB" id="1910at2157"/>
    <dbReference type="PhylomeDB" id="Q58598"/>
    <dbReference type="EvolutionaryTrace" id="Q58598"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10719">
        <property type="entry name" value="DnaJ_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001305">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036410">
        <property type="entry name" value="HSP_DnaJ_Cys-rich_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57938">
        <property type="entry name" value="SSF57938"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51188">
        <property type="entry name" value="ZF_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000107211" description="Uncharacterized protein MJ1198">
        <location>
            <begin position="1"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="135"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="CR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="149"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="158"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="180"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="190"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="194"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00546"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2K52"/>
        </source>
    </evidence>
    <sequence length="761" mass="85939" checksum="FB8C3D16923E2E12" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58618</accession>
    <name>Y1221_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1221</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1221</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0678.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64452">
        <property type="entry name" value="D64452"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58618"/>
    <dbReference type="SMR" id="Q58618"/>
    <dbReference type="STRING" id="243232.MJ_1221"/>
    <dbReference type="EnsemblBacteria" id="AAB99234">
        <property type="protein sequence ID" value="AAB99234"/>
        <property type="gene ID" value="MJ_1221"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1221"/>
    <dbReference type="eggNOG" id="arCOG02264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_945320_0_0_2"/>
    <dbReference type="InParanoid" id="Q58618"/>
    <dbReference type="OMA" id="TIMPANI"/>
    <dbReference type="PhylomeDB" id="Q58618"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="PTHR20992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107222" description="Uncharacterized protein MJ1221">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="299" mass="32538" checksum="82A1E3BE398C9DFF" modified="1996-11-01" version="1">MWFGERMRYMKIIIPKKFLNTVEEILKKNNAYSISIIEPLKTSIEDGIIITCNADARDAEKIVLELKKLGLGEKGHGSVTIMPANITFSCREEGIASTSLSPLELYYKAKTMVKITKNVIIKVILASIMGVIGLIEHNIPTLIGAMIIAPLVDTVMGSAIGTVLGDKELFIQGMKKELLCSGIVIVCAFIPSLFFVSKELVLQYLSETSIILSAIVAIIAGISGGMSIASGKEYEIIGVTIDVSILIPALLMGMALATMDLYLIYITFILLAINIVLLDVGGYIGLKYKVGKINQKIKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58619</accession>
    <name>Y1222_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1222</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1222</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64452">
        <property type="entry name" value="E64452"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58619"/>
    <dbReference type="SMR" id="Q58619"/>
    <dbReference type="STRING" id="243232.MJ_1222"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99226">
        <property type="protein sequence ID" value="AAB99226"/>
        <property type="gene ID" value="MJ_1222"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1222"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_7_4_2"/>
    <dbReference type="InParanoid" id="Q58619"/>
    <dbReference type="OMA" id="IYTEYSM"/>
    <dbReference type="PhylomeDB" id="Q58619"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107223" description="Uncharacterized protein MJ1222">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <sequence length="243" mass="27428" checksum="23BE405026EA5A44" modified="1996-11-01" version="1">MEIMKKQNSQINEINKDEIFVVVPAFNEEKMIGETLKNLKKEGYKNIVVVDDGSMDKTSEIAKKEGVIVCRHILNRGLGGALGTGIKCALLYKPKIIITFDADGQHHPKDVEKVVKPVLFEGYDMAIGSRMMDKNELKNMPLVKRIGNFGLNFITYLMGGYFVTDSQSGLRAFSYEAAKKIIGDLKSDRYEVSSEFIILAKKHGLKLKEVPIKTIYTEYSMSRGTNVITGFKILFKLIMQKIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58620</accession>
    <name>Y1223_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1223</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1223</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.thermoautotrophicum MTH1250.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64452">
        <property type="entry name" value="F64452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870735.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58620"/>
    <dbReference type="SMR" id="Q58620"/>
    <dbReference type="STRING" id="243232.MJ_1223"/>
    <dbReference type="EnsemblBacteria" id="AAB99235">
        <property type="protein sequence ID" value="AAB99235"/>
        <property type="gene ID" value="MJ_1223"/>
    </dbReference>
    <dbReference type="GeneID" id="1452119"/>
    <dbReference type="KEGG" id="mja:MJ_1223"/>
    <dbReference type="eggNOG" id="arCOG04878">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189621_0_0_2"/>
    <dbReference type="OMA" id="FAFCKVI"/>
    <dbReference type="OrthoDB" id="115824at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107224" description="Uncharacterized protein MJ1223">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="92" mass="9739" checksum="DDBCA78E28CCF164" modified="1996-11-01" version="1">MNIYVWLFAIIALSFSALVGLRLSFKKGTANVLVGESIITVVAGTLIVVISQKYNLAFADTIALAIFICGVVGAFAFCKVIGGDNEKAKQPN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58630</accession>
    <name>Y1233_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1233</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1233</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64453">
        <property type="entry name" value="H64453"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58630"/>
    <dbReference type="SMR" id="Q58630"/>
    <dbReference type="STRING" id="243232.MJ_1233"/>
    <dbReference type="EnsemblBacteria" id="AAB99243">
        <property type="protein sequence ID" value="AAB99243"/>
        <property type="gene ID" value="MJ_1233"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1233"/>
    <dbReference type="eggNOG" id="arCOG00054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057700_0_0_2"/>
    <dbReference type="InParanoid" id="Q58630"/>
    <dbReference type="OMA" id="LDTCMGL"/>
    <dbReference type="PhylomeDB" id="Q58630"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107229" description="Uncharacterized protein MJ1233">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <sequence length="288" mass="32912" checksum="E8050E04ECF009DA" modified="1996-11-01" version="1">MSKITLVLTMNYITSKIAKEILNSQSEEIFLNLDLNKTEKKEKILIDREKKIAKFPEGDVSFDILKKIAKDEGHIYFIKDGEVFKAAISNNGYYKLVPTIPPTIEINGIRMHRTKEVNPYEDTLNKINAVKVKKGEKVLDTCMGLGYTAIEAYRRGAEVITIEKNPNVLELAKINPYSEELFKGGIKIILGDAYDVIKRFKDEEFDVVIHDPPRFSLAGHLYSEEFYKEIFRVLKPGGRLFHYVGNPGKKYRGKDLQKGVMERLRKVGFVNVKRVEEALGVVAVKPRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58636</accession>
    <name>Y1239_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1239</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1239</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64454">
        <property type="entry name" value="F64454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870751.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58636"/>
    <dbReference type="STRING" id="243232.MJ_1239"/>
    <dbReference type="EnsemblBacteria" id="AAB99244">
        <property type="protein sequence ID" value="AAB99244"/>
        <property type="gene ID" value="MJ_1239"/>
    </dbReference>
    <dbReference type="GeneID" id="1452135"/>
    <dbReference type="KEGG" id="mja:MJ_1239"/>
    <dbReference type="eggNOG" id="arCOG05074">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1100981_0_0_2"/>
    <dbReference type="OMA" id="SYDFDIC"/>
    <dbReference type="OrthoDB" id="59518at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107232" description="Uncharacterized protein MJ1239">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <sequence length="225" mass="26424" checksum="FD0677EE457AB5AF" modified="1996-11-01" version="1">MIGIAIKPSKINEDIVDFGHFIGRYFDYYIIDAENEVIKDEEILERLEKISKVVMTVQAKRVDAFELLELYASYGFDLCVVLGNKQYLTEKERKFKNYRILDVIKEAMRCSRDIWVGIEGVESLVKDIVEEHNLIAYYLYGQECSINSKRAIYVPYATKINENALESMKGYLNRRKNYRGNWRDFILSLNDEESIERIKNNDIVVGYPIIPNKEEILNFVRCFSV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58640</accession>
    <name>Y1243_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0251 protein MJ1243</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1243</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0251 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64455">
        <property type="entry name" value="B64455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870755.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58640"/>
    <dbReference type="STRING" id="243232.MJ_1243"/>
    <dbReference type="PRIDE" id="Q58640"/>
    <dbReference type="EnsemblBacteria" id="AAB99248">
        <property type="protein sequence ID" value="AAB99248"/>
        <property type="gene ID" value="MJ_1243"/>
    </dbReference>
    <dbReference type="GeneID" id="1452139"/>
    <dbReference type="KEGG" id="mja:MJ_1243"/>
    <dbReference type="eggNOG" id="arCOG02238">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094511_0_1_2"/>
    <dbReference type="InParanoid" id="Q58640"/>
    <dbReference type="OMA" id="VDYLDYT"/>
    <dbReference type="OrthoDB" id="77586at2157"/>
    <dbReference type="PhylomeDB" id="Q58640"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00674">
        <property type="entry name" value="UPF0251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002852">
        <property type="entry name" value="UPF0251"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37478">
        <property type="entry name" value="PTHR37478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02001">
        <property type="entry name" value="DUF134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147576" description="UPF0251 protein MJ1243">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="123" mass="14391" checksum="2DC553451B5E1F19" modified="1996-11-01" version="1">MSRRGRPKIPRFISEEPKFRIFKPHGVSLTEVDKVILSVDELEAIRLVDYLDYTQEEASKLMGISRRVLWSLLTEGRKKIADALINGKAIVIEGGEYKIRECGFCMRHRFGIKKHCRTWREEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58675</accession>
    <name>Y1279_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1279</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1279</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64459">
        <property type="entry name" value="F64459"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58675"/>
    <dbReference type="STRING" id="243232.MJ_1279"/>
    <dbReference type="PRIDE" id="Q58675"/>
    <dbReference type="EnsemblBacteria" id="AAB99285">
        <property type="protein sequence ID" value="AAB99285"/>
        <property type="gene ID" value="MJ_1279"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1279"/>
    <dbReference type="eggNOG" id="arCOG10917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1574965_0_0_2"/>
    <dbReference type="OMA" id="VIGARMF"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107247" description="Uncharacterized protein MJ1279">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <sequence length="185" mass="21379" checksum="8727A0497D57DDC1" modified="1996-11-01" version="1">MVILMDKRFIELIKKGWKLKNEENKATYIDEVFLGAIITTLTDNGYVLMDIASNGNFHYFMFEHLESWDRIKIVAEVLPHSLTDVKVIGARMFIEFSYGVMIKGIPPSLFGLGLKGYLSQMLSNIGSIRYEYDGYYTFVNCATYLLINDYIDFDTLTIDWEKLNNDINAIISSLAKYLEIHKKVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58686</accession>
    <name>Y1290_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1290</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1290</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64461">
        <property type="entry name" value="A64461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870806.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58686"/>
    <dbReference type="STRING" id="243232.MJ_1290"/>
    <dbReference type="EnsemblBacteria" id="AAB99296">
        <property type="protein sequence ID" value="AAB99296"/>
        <property type="gene ID" value="MJ_1290"/>
    </dbReference>
    <dbReference type="GeneID" id="1452191"/>
    <dbReference type="KEGG" id="mja:MJ_1290"/>
    <dbReference type="eggNOG" id="arCOG08273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978634_0_0_2"/>
    <dbReference type="OMA" id="GKYILEY"/>
    <dbReference type="OrthoDB" id="81240at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107257" description="Uncharacterized protein MJ1290">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="312" mass="35823" checksum="0E36803803E7605B" modified="1998-06-01" version="2">MKKAIYLLILCIFGLFSVYFTYAENISDISNTTSKNISSSNISHNNIIYSNINYNEILYIIVKNNTAYVKDVINGTNNPYHIKSAGIILYEKIYGYNYSNLLYRNSSNSLIFYYNFSVDKINYTINITIPQIEDYVGSLGGPIRMRIPPNNVKIIIVAENKLAETNGKYILEYNKTDKKVISLIYLDNVSSICNIYYTKFFNSSEFYGYAVANVTSITENRTSYTIKNPKGTFTFDRKYNVFVSNKTAYLKEPYLYVKLYNSTIDDIIILENNKISENSTKFMSNYLLSFIGIIIGFGIIGLAIYLSKRGRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58698</accession>
    <name>Y1302_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized polyferredoxin-like protein MJ1302</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1302</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64462">
        <property type="entry name" value="E64462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870819.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58698"/>
    <dbReference type="STRING" id="243232.MJ_1302"/>
    <dbReference type="EnsemblBacteria" id="AAB99311">
        <property type="protein sequence ID" value="AAB99311"/>
        <property type="gene ID" value="MJ_1302"/>
    </dbReference>
    <dbReference type="GeneID" id="1452204"/>
    <dbReference type="KEGG" id="mja:MJ_1302"/>
    <dbReference type="eggNOG" id="arCOG01543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108340_0_0_2"/>
    <dbReference type="InParanoid" id="Q58698"/>
    <dbReference type="OMA" id="YCHDFCP"/>
    <dbReference type="OrthoDB" id="108931at2157"/>
    <dbReference type="PhylomeDB" id="Q58698"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159152" description="Uncharacterized polyferredoxin-like protein MJ1302">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="91"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="168" mass="19169" checksum="6BBC523D3DB9AD03" modified="1996-11-01" version="1">MVIELAELKNFAKIFLTGIYENLERIIFGSGRYTSLEMRNAILTGTVKIPKTVIEELCIGCEGCANVCPTKAIEMIPIEPVKITDNYVKDKIPKINPEKCVYCLYCHDFCPVFSVFNEISPIHPRDVGEEYIEIDISKLLQKKIEISEEQINKISSLLSINLRRIIKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58714</accession>
    <name>Y1318_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1318</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1318</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S16 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64464">
        <property type="entry name" value="E64464"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58714"/>
    <dbReference type="SMR" id="Q58714"/>
    <dbReference type="STRING" id="243232.MJ_1318"/>
    <dbReference type="MEROPS" id="S16.A12"/>
    <dbReference type="EnsemblBacteria" id="AAB99325">
        <property type="protein sequence ID" value="AAB99325"/>
        <property type="gene ID" value="MJ_1318"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1318"/>
    <dbReference type="eggNOG" id="arCOG01937">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027628_0_0_2"/>
    <dbReference type="InParanoid" id="Q58714"/>
    <dbReference type="OMA" id="VMMTGMI"/>
    <dbReference type="PhylomeDB" id="Q58714"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107273" description="Uncharacterized protein MJ1318">
        <location>
            <begin position="1"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="2">
        <location>
            <begin position="25"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="602" mass="67257" checksum="A5FBE1C388160AA3" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58739</accession>
    <name>AMT2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ammonium transporter Amt2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">amt2</name>
        <name type="ordered locus">MJ1343</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3)) (By similarity). Transport is electrogenic.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer (By similarity). Interacts and forms a complex with GlnK2.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-7201308">
            <id>Q58739</id>
        </interactant>
        <interactant intactId="EBI-7201321">
            <id>Q60381</id>
            <label>glnK1</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64467">
        <property type="entry name" value="F64467"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58739"/>
    <dbReference type="SMR" id="Q58739"/>
    <dbReference type="IntAct" id="Q58739">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="Q58739"/>
    <dbReference type="STRING" id="243232.MJ_1343"/>
    <dbReference type="EnsemblBacteria" id="AAB99352">
        <property type="protein sequence ID" value="AAB99352"/>
        <property type="gene ID" value="MJ_1343"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1343"/>
    <dbReference type="eggNOG" id="arCOG04397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_2"/>
    <dbReference type="InParanoid" id="Q58739"/>
    <dbReference type="OMA" id="IWLSIMS"/>
    <dbReference type="PhylomeDB" id="Q58739"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0924">Ammonia transport</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000139765" description="Ammonium transporter Amt2">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="339"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="site" description="Twin-His motif. Important for optimum substrate conductance" evidence="2">
        <location>
            <position position="186"/>
        </location>
    </feature>
    <feature type="site" description="Twin-His motif. Important for optimum substrate conductance" evidence="2">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O29285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P69681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q60366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4"/>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="420" mass="43786" checksum="F7AE2554D19DACA2" modified="1997-11-01" version="1">MWGENIATADLFANATDIHSIVQALTTLANASDVFFLVVMGVLVFMMQWGFAMLEGGQVRKKNVNNVMMKNMVDWLIGCVAWLFIGGILCSKGFDLSAFIDWWKQILGTNWPNNGLDLASWFFGLVFCATAATIVSGGVAERIKFSAYVLISLIITGLLYPLFVYLGPWGASIVPWHDYAGSLVVHGLGGFLALGAIAALGPRIGRFVDGRPVPILGHNIPMAVFGAFALAIGWYGFNVGSSLALGDISGLVCATTTMAMAGGGIGALIASRNDVLFTANGIVAGLVAICSGTDVVSPIGGLIIGLIAGLQVPIVYKLVEKAGLDDVCGVVPVHGTAGVIGAILTGILGLKIFGGAGGVSLIDQIIGAVFCIIYGTGLGYILAKIVGIALGGLRVSEEEEKMGLDMAEHKMPAYPEETVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58764</accession>
    <name>Y1369_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1369</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1369</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64470">
        <property type="entry name" value="H64470"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58764"/>
    <dbReference type="SMR" id="Q58764"/>
    <dbReference type="STRING" id="243232.MJ_1369"/>
    <dbReference type="EnsemblBacteria" id="AAB99386">
        <property type="protein sequence ID" value="AAB99386"/>
        <property type="gene ID" value="MJ_1369"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1369"/>
    <dbReference type="eggNOG" id="arCOG02727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1691555_0_0_2"/>
    <dbReference type="OMA" id="FPWRVSF"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107301" description="Uncharacterized protein MJ1369">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <sequence length="155" mass="18595" checksum="CC47AC47A0A684EB" modified="1996-11-01" version="1">MRDIVMTYWLFSSNNIRNIEICYNHMIWGFWDRNAGEKQKKNWRSFIRKYNQIKPFDVAVFQIAKTGEIHAIGVIKETYYDDQTPIWDNEINLNKVTFPWRVSFSVIIFSKEAVIKRFIKIQDYIDGYGLGELEHHDFNEILKAFQKKFGMISIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-02-23" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58791</accession>
    <name>Y1396_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1396</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1396</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64474">
        <property type="entry name" value="C64474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870913.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1396"/>
    <dbReference type="EnsemblBacteria" id="AAB99406">
        <property type="protein sequence ID" value="AAB99406"/>
        <property type="gene ID" value="MJ_1396"/>
    </dbReference>
    <dbReference type="GeneID" id="1452299"/>
    <dbReference type="KEGG" id="mja:MJ_1396"/>
    <dbReference type="eggNOG" id="arCOG02499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_228138_0_0_2"/>
    <dbReference type="InParanoid" id="Q58791"/>
    <dbReference type="OMA" id="TWNKDIT"/>
    <dbReference type="OrthoDB" id="10at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039448">
        <property type="entry name" value="Beta_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006626">
        <property type="entry name" value="PbH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13229">
        <property type="entry name" value="Beta_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00710">
        <property type="entry name" value="PbH1"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="SSF49899"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="SSF51126"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107311" description="Uncharacterized protein MJ1396">
        <location>
            <begin position="1"/>
            <end position="2894"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 1">
        <location>
            <begin position="543"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 2">
        <location>
            <begin position="2085"/>
            <end position="2107"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 3">
        <location>
            <begin position="2135"/>
            <end position="2156"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 4">
        <location>
            <begin position="2158"/>
            <end position="2180"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 5">
        <location>
            <begin position="2201"/>
            <end position="2223"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 6">
        <location>
            <begin position="2224"/>
            <end position="2244"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 7">
        <location>
            <begin position="2245"/>
            <end position="2266"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 8">
        <location>
            <begin position="2267"/>
            <end position="2289"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 9">
        <location>
            <begin position="2290"/>
            <end position="2311"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 10">
        <location>
            <begin position="2341"/>
            <end position="2363"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 11">
        <location>
            <begin position="2367"/>
            <end position="2389"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 12">
        <location>
            <begin position="2390"/>
            <end position="2419"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 13">
        <location>
            <begin position="2422"/>
            <end position="2444"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 14">
        <location>
            <begin position="2455"/>
            <end position="2477"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 15">
        <location>
            <begin position="2479"/>
            <end position="2501"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 16">
        <location>
            <begin position="2512"/>
            <end position="2542"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 17">
        <location>
            <begin position="2550"/>
            <end position="2582"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 18">
        <location>
            <begin position="2589"/>
            <end position="2611"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 19">
        <location>
            <begin position="2612"/>
            <end position="2633"/>
        </location>
    </feature>
    <feature type="repeat" description="PbH1 20">
        <location>
            <begin position="2638"/>
            <end position="2660"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="2894" mass="322363" checksum="8B64F786537A16DF" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58796</accession>
    <name>Y1401_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable ATP-dependent helicase MJ1401</fullName>
            <ecNumber>3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1401</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64474">
        <property type="entry name" value="H64474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870918.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58796"/>
    <dbReference type="SMR" id="Q58796"/>
    <dbReference type="STRING" id="243232.MJ_1401"/>
    <dbReference type="EnsemblBacteria" id="AAB99409">
        <property type="protein sequence ID" value="AAB99409"/>
        <property type="gene ID" value="MJ_1401"/>
    </dbReference>
    <dbReference type="GeneID" id="1452304"/>
    <dbReference type="KEGG" id="mja:MJ_1401"/>
    <dbReference type="eggNOG" id="arCOG00554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010611_0_0_2"/>
    <dbReference type="InParanoid" id="Q58796"/>
    <dbReference type="OMA" id="SRRRCHE"/>
    <dbReference type="OrthoDB" id="3149at2157"/>
    <dbReference type="PhylomeDB" id="Q58796"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043852">
        <property type="entry name" value="DUF5814"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19131">
        <property type="entry name" value="DUF5814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102014" description="Probable ATP-dependent helicase MJ1401">
        <location>
            <begin position="1"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="221"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="396"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif">
        <location>
            <begin position="189"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEIH box">
        <location>
            <begin position="336"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="234"/>
            <end position="241"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="808" mass="92933" checksum="C4F0DD8299BC0F0C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58798</accession>
    <name>Y1403_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1403</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1403</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64475">
        <property type="entry name" value="B64475"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870920.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58798"/>
    <dbReference type="SMR" id="Q58798"/>
    <dbReference type="EnsemblBacteria" id="AAB99413">
        <property type="protein sequence ID" value="AAB99413"/>
        <property type="gene ID" value="MJ_1403"/>
    </dbReference>
    <dbReference type="GeneID" id="1452306"/>
    <dbReference type="KEGG" id="mja:MJ_1403"/>
    <dbReference type="eggNOG" id="arCOG05987">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775263_0_0_2"/>
    <dbReference type="OMA" id="HLNDNYQ"/>
    <dbReference type="OrthoDB" id="48031at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107312" description="Uncharacterized protein MJ1403">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="374" mass="41002" checksum="BC56B019D75D7E59" modified="1996-11-01" version="1">MTVSHGGILEGSSRGGKMMDWLKNKKAISPILALLIVLGVTIVVGAVFYAWGSNLFGNSQEKTQAAVEGTATNMFYDAGAIRVAATCIDKIRYQDADDSDSWLGYPNGNGKIAKPSTSNGCYNSTYGTVFYDERFIVEIPVTIDTQDYKLTGVKVVGGIPKIVDMGGTYTNAFEDISAKFYAFWLHLNDNYQLLKKDGTLFVGYVNKSGMFEVSNGYVIAWNQTRDTYGKLASSVGATSDSSWDAVNTTTGVAPLVETSWPYYGTYCSNVKLYTATGEELKPGFGSGTLVAQWFCSSATYLDKLFNNPEYVVGTLPKNSEKTVKTYLFFNTLYLPNYKGSTNDGYVTFEVPLKVVSNEGVTKEVKVKFTVYDDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58813</accession>
    <name>FUCA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-fuculose phosphate aldolase</fullName>
            <ecNumber evidence="2 3 4">4.1.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">L-fuculose-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">fucA</name>
        <name type="ordered locus">MJ1418</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="J. Microbiol. Biotechnol." volume="11" first="709" last="711">
            <title>Mutagenic characterization of a conserved functional amino acid in fuculose-1-phosphate aldolase from Methanococcus jannaschii, a hyperthermophic archaea.</title>
            <authorList>
                <person name="Yoon H.-S."/>
                <person name="Kwon S.-J."/>
                <person name="Han M.-S."/>
                <person name="Yu Y.-G."/>
                <person name="Yoon M.-Y."/>
            </authorList>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF ASN-25</scope>
        <scope>COFACTOR</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="J. Bacteriol." volume="188" first="2836" last="2844">
            <title>Identification of lactaldehyde dehydrogenase in Methanocaldococcus jannaschii and its involvement in production of lactate for F420 biosynthesis.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="16585745"/>
            <dbReference type="DOI" id="10.1128/JB.188.8.2836-2844.2006"/>
        </citation>
        <scope>FUNCTION IN F420 BIOSYNTHESIS</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="J. Biochem. Mol. Biol." volume="40" first="801" last="804">
            <title>Characterization of aldolase from Methanococcus jannaschii by gas chromatography.</title>
            <authorList>
                <person name="Nam Shin J.E."/>
                <person name="Kim M.J."/>
                <person name="Choi J.A."/>
                <person name="Chun K.H."/>
            </authorList>
            <dbReference type="PubMed" id="17927915"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2012" name="Enzyme Microb. Technol." volume="50" first="209" last="214">
            <title>Kinetic mechanism of fuculose-1-phosphate aldolase from the hyperthermophilic archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Park H.C."/>
                <person name="Park J.S."/>
                <person name="Choi J.D."/>
                <person name="Dabrowski M."/>
                <person name="Atkins W.M."/>
                <person name="Yoon M.Y."/>
            </authorList>
            <dbReference type="PubMed" id="22418259"/>
            <dbReference type="DOI" id="10.1016/j.enzmictec.2012.01.001"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 4 5">Involved in the biosynthesis of the coenzyme F420 which requires phospholactate produced via the aldol cleavage of L-fuculose 1-phosphate and the NAD(+)-dependent oxidation of (S)-lactaldehyde (PubMed:16585745). Catalyzes the reversible cleavage of L-fuculose 1-phosphate (Fuc1P) to yield dihydroxyacetone phosphate (DHAP) and S-lactaldehyde (Ref.2, PubMed:16585745, PubMed:17927915, PubMed:22418259). FucA possesses a high specificity for the dihydroxyacetone phosphate (DHAP), but accepts a great variety of different aldehydes such as DL-glyceraldehyde and glycolaldehyde (PubMed:16585745, PubMed:17927915).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3 4 5">
            <text>L-fuculose 1-phosphate = (S)-lactaldehyde + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:12933"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57846"/>
            <dbReference type="EC" id="4.1.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Trimethyl phosphonoacetate and DL-threose are competitive inhibitors with respect to dihydroxyacetone phosphate, and uncompetitive inhibitors with respect to DL-glyceraldehyde.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="4">90 uM for dihydroxyacetone phosphate (DHAP)</KM>
            <KM evidence="4">743 uM for DL-glyceraldehyde</KM>
            <KM evidence="5">7.8 mM for DL-glyceraldehyde (DHAP)</KM>
            <KM evidence="5">1.01 mM for dihydroxyacetone phosphate (DHAP)</KM>
            <Vmax evidence="2">0.026 umol/min/mg enzyme (in the direction of aldol condensation)</Vmax>
            <Vmax evidence="2">0.013 umol/min/mg enzyme (in the direction of hydrolysis)</Vmax>
            <Vmax evidence="2">570.0 nmol/min/mg enzyme with DL-lactaldehyde as substrate (at pH 8 and at 70 degrees Celsius)</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the aldolase class II family. AraD/FucA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.17" evidence="2 3 4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64477">
        <property type="entry name" value="A64477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870936.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58813"/>
    <dbReference type="SMR" id="Q58813"/>
    <dbReference type="STRING" id="243232.MJ_1418"/>
    <dbReference type="EnsemblBacteria" id="AAB99428">
        <property type="protein sequence ID" value="AAB99428"/>
        <property type="gene ID" value="MJ_1418"/>
    </dbReference>
    <dbReference type="GeneID" id="1452322"/>
    <dbReference type="KEGG" id="mja:MJ_1418"/>
    <dbReference type="eggNOG" id="arCOG04226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006033_3_1_2"/>
    <dbReference type="InParanoid" id="Q58813"/>
    <dbReference type="OMA" id="ICRYGRS"/>
    <dbReference type="OrthoDB" id="101802at2157"/>
    <dbReference type="PhylomeDB" id="Q58813"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-12176"/>
    <dbReference type="BRENDA" id="4.1.2.17">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008738">
        <property type="term" value="F:L-fuculose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000162932" description="L-fuculose phosphate aldolase">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="24"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="39"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="66"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="It shows a 3-fold increase of the affinty for dihydroxyacetone phosphate (DHAP) and a 3-fold decrease of the affinity for DL-glyceraldehyde compared to the wild-type." evidence="5">
        <original>N</original>
        <variation>L</variation>
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="It shows a 5-fold decrease of the affinty for dihydroxyacetone phosphate (DHAP), but has the same affinity for DL-glyceraldehyde compared to the wild-type." evidence="5">
        <original>N</original>
        <variation>T</variation>
        <location>
            <position position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0AB87"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16585745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17927915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22418259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source ref="2"/>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="16585745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="16585745"/>
        </source>
    </evidence>
    <sequence length="181" mass="20470" checksum="E5F3BF13722145B0" modified="1996-11-01" version="1">MDKKQFIKICRKLYDRKYVVGSGGNVSVKEGDKIYLTPTGSILGFLKEDDIAEMDLDGNVIKGKPTSEKNLHLMIYRKRNDINAIIHTHSLISTFLSTINKEIELLTPEGKIFLKKIGYVDYYEAGSLKLAEETAKRDEDVIILKNHGVVCLGKDLIDAYIKVEVLEEQAKLTLLNLLVKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58842</accession>
    <name>FAEHP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Bifunctional enzyme Fae/Hps</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">5,6,7,8-tetrahydromethanopterin hydro-lyase</fullName>
                <ecNumber evidence="1">4.2.1.147</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1 3">Formaldehyde-activating enzyme</fullName>
                <shortName evidence="1 3">Fae</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1 3">3-hexulose-6-phosphate synthase</fullName>
                <shortName evidence="1 3">HPS</shortName>
                <ecNumber evidence="1 2">4.1.2.43</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="1">D-arabino-3-hexulose-6-phosphate formaldehyde lyase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="1" type="primary">fae-hps</name>
        <name type="ordered locus">MJ1447</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Bacteriol." volume="187" first="7382" last="7389">
            <title>Ribose-5-phosphate biosynthesis in Methanocaldococcus jannaschii occurs in the absence of a pentose-phosphate pathway.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="16237021"/>
            <dbReference type="DOI" id="10.1128/JB.187.21.7382-7389.2005"/>
        </citation>
        <scope>FUNCTION AS A HPS</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Catalyzes the condensation of formaldehyde with tetrahydromethanopterin (H(4)MPT) to 5,10-methylenetetrahydromethanopterin.</text>
    </comment>
    <comment type="function">
        <text evidence="1 2">Catalyzes the reversible formation of ribulose-5-phosphate and formaldehyde from 3-hexulose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,6,7,8-tetrahydromethanopterin + formaldehyde = 5,10-methylenetetrahydromethanopterin + H2O</text>
            <dbReference type="Rhea" id="RHEA:24678"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="EC" id="4.2.1.147"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>D-ribulose 5-phosphate + formaldehyde = D-arabino-hex-3-ulose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:25201"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58542"/>
            <dbReference type="EC" id="4.1.2.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 2">Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the formaldehyde-activating enzyme family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the HPS/KGPDC family. HPS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Ile-16 is present instead of the conserved His which is expected to be an active site residue. Substrate-binding sites are also not conserved. Thus, this enzyme may not display fae activity.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.147" evidence="1"/>
    <dbReference type="EC" id="4.1.2.43" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64480">
        <property type="entry name" value="F64480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870967.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58842"/>
    <dbReference type="SMR" id="Q58842"/>
    <dbReference type="STRING" id="243232.MJ_1447"/>
    <dbReference type="DNASU" id="1452351"/>
    <dbReference type="EnsemblBacteria" id="AAB99456">
        <property type="protein sequence ID" value="AAB99456"/>
        <property type="gene ID" value="MJ_1447"/>
    </dbReference>
    <dbReference type="GeneID" id="1452351"/>
    <dbReference type="KEGG" id="mja:MJ_1447"/>
    <dbReference type="eggNOG" id="arCOG00103">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_701335_0_0_2"/>
    <dbReference type="InParanoid" id="Q58842"/>
    <dbReference type="OMA" id="LWDPPYL"/>
    <dbReference type="OrthoDB" id="24053at2157"/>
    <dbReference type="PhylomeDB" id="Q58842"/>
    <dbReference type="UniPathway" id="UPA00293"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033982">
        <property type="term" value="F:3-dehydro-L-gulonate-6-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043801">
        <property type="term" value="F:hexulose-6-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019854">
        <property type="term" value="P:L-ascorbic acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01268">
        <property type="entry name" value="Fae_Hps"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020868">
        <property type="entry name" value="Fae/Hps"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014826">
        <property type="entry name" value="HCHO-activating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037075">
        <property type="entry name" value="HCHO-activating_enzyme_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08714">
        <property type="entry name" value="Fae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000212107" description="Bifunctional enzyme Fae/Hps">
        <location>
            <begin position="1"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Formaldehyde-activating enzyme" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-hexulose-6-phosphate synthase" evidence="1">
        <location>
            <begin position="151"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16237021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="16237021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="381" mass="42990" checksum="BB4C460AE796FAA3" modified="1996-11-01" version="1">MIKFGEAVLGNEIKAIVNVALGKGELIENTFTNALTRGNCVFANLRPNLIVKPLTLVVPRHNIESEIQDELFQGVIQYAVAKAVADLDLDEDLKVVVSVNVPEVPITNLNKRKLFQYFYASAKLAINRALNEYPSKEKVKKEKYRALHPLVGFRDVRLEYPPYLQIALDVPTMENLEFLLQTIPNSDHIILEAGTPLIKKFGLEVIEIMREYFDGFIVADLKTLDTGRVEVRLAFEATANAVAISGVAPKSTIIKAIHECQKCGLISYLDMMNVSEPQKLYDSLKLKPDVVILHRGIDEETFGIKKEWKFKENCLLAIAGGVGVENVEELLKEYQILIVGRAITKSKDPGRVIRMFINKMGYDIDTYRLYFDEDEDIGEEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58862</accession>
    <name>Y1467_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1467</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1467</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64483">
        <property type="entry name" value="B64483"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58862"/>
    <dbReference type="STRING" id="243232.MJ_1467"/>
    <dbReference type="PRIDE" id="Q58862"/>
    <dbReference type="EnsemblBacteria" id="AAB99483">
        <property type="protein sequence ID" value="AAB99483"/>
        <property type="gene ID" value="MJ_1467"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1467"/>
    <dbReference type="eggNOG" id="arCOG06584">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125809_0_0_2"/>
    <dbReference type="OMA" id="NVLNMAK"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107353" description="Uncharacterized protein MJ1467">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <sequence length="184" mass="20993" checksum="5659B7F6E9401828" modified="1996-11-01" version="1">MEKYKLRKNNTILLYDYSINFSIKDNVKLEKKRFNMNKNKIKKRGQLSVDFVLAILFLMLVSLFIYYNALTFTNNTTDALIVDRMYSIADTFENYAILSYTKNETIVLKLKPIGDLGYVIHVSNKIINVSYKTLIVFTPTDNGVIISGSNIETAPVDIGKNISITVTIDKNNITICKELTINIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58917</accession>
    <name>CBIE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable cobalt-precorrin-7 C(5)-methyltransferase</fullName>
            <ecNumber>2.1.1.289</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cobalt-precorrin-6Y C(5)-methyltransferase</fullName>
            <shortName>Cobalt-precorrin-6 methyltransferase</shortName>
            <shortName>Cobalt-precorrin-6Y methylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cbiE</name>
        <name type="synonym">cobL</name>
        <name type="ordered locus">MJ1522</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-5 in cobalt-precorrin-7 to form cobalt-precorrin-8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Co-precorrin-7 + S-adenosyl-L-methionine = Co-precorrin-8X + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:34591"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:70792"/>
            <dbReference type="EC" id="2.1.1.289"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.289"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64490">
        <property type="entry name" value="A64490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871046.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58917"/>
    <dbReference type="SMR" id="Q58917"/>
    <dbReference type="STRING" id="243232.MJ_1522"/>
    <dbReference type="EnsemblBacteria" id="AAB99541">
        <property type="protein sequence ID" value="AAB99541"/>
        <property type="gene ID" value="MJ_1522"/>
    </dbReference>
    <dbReference type="GeneID" id="1452430"/>
    <dbReference type="KEGG" id="mja:MJ_1522"/>
    <dbReference type="eggNOG" id="arCOG00650">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089162_2_0_2"/>
    <dbReference type="InParanoid" id="Q58917"/>
    <dbReference type="OMA" id="DPCFSGL"/>
    <dbReference type="OrthoDB" id="90646at2157"/>
    <dbReference type="PhylomeDB" id="Q58917"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043777">
        <property type="term" value="F:cobalt-precorrin-7 C15-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150410" description="Probable cobalt-precorrin-7 C(5)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="211" mass="23806" checksum="279A1A2B14369510" modified="1996-11-01" version="1">MIYIVGIGPGDREYLTLKAIKIVENADLVVGSKRALELFNIDEDKKITLTKNLIGELKELIKNENIKNKKIAILSTGDPCFSGLLKTLLKIGAKKEDIEAISGISSIQIAAAKLKISWEDYYIITLHGKEENRKKLLNLIKNHEKVIFLPNNLKEDAKFLINNGINPDTKIWVLENLTYENEKISLKSLKEIANGDFSYLTVCVYEGDEEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58937</accession>
    <name>Y1542_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1542</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1542</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64492">
        <property type="entry name" value="E64492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871066.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58937"/>
    <dbReference type="STRING" id="243232.MJ_1542"/>
    <dbReference type="EnsemblBacteria" id="AAB99570">
        <property type="protein sequence ID" value="AAB99570"/>
        <property type="gene ID" value="MJ_1542"/>
    </dbReference>
    <dbReference type="GeneID" id="1452450"/>
    <dbReference type="KEGG" id="mja:MJ_1542"/>
    <dbReference type="eggNOG" id="arCOG03240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_367870_0_0_2"/>
    <dbReference type="InParanoid" id="Q58937"/>
    <dbReference type="OMA" id="YSKENPW"/>
    <dbReference type="OrthoDB" id="11692at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13304">
        <property type="entry name" value="AAA_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107399" description="Uncharacterized protein MJ1542">
        <location>
            <begin position="1"/>
            <end position="808"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="808" mass="95564" checksum="CD716B4B9AB7B04C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58946</accession>
    <name>Y1551_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1551</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1551</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64493">
        <property type="entry name" value="F64493"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58946"/>
    <dbReference type="SMR" id="Q58946"/>
    <dbReference type="STRING" id="243232.MJ_1551"/>
    <dbReference type="EnsemblBacteria" id="AAB99571">
        <property type="protein sequence ID" value="AAB99571"/>
        <property type="gene ID" value="MJ_1551"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1551"/>
    <dbReference type="eggNOG" id="arCOG04856">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200895_3_0_2"/>
    <dbReference type="OMA" id="CSCNLYF"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019300">
        <property type="entry name" value="CooT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10133">
        <property type="entry name" value="CooT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107405" description="Uncharacterized protein MJ1551">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <sequence length="57" mass="6515" checksum="0B6F2B918406A96C" modified="1996-11-01" version="1">MCSCNLYFNGELVMEDVMIVEKKGDKVIAIDLFGDKKEFVGEIKKIDLNENKIFIEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58977</accession>
    <name>Y1582_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Uncharacterized metal-dependent hydrolase MJ1582</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1582</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64497">
        <property type="entry name" value="E64497"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871107.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58977"/>
    <dbReference type="SMR" id="Q58977"/>
    <dbReference type="STRING" id="243232.MJ_1582"/>
    <dbReference type="EnsemblBacteria" id="AAB99602">
        <property type="protein sequence ID" value="AAB99602"/>
        <property type="gene ID" value="MJ_1582"/>
    </dbReference>
    <dbReference type="GeneID" id="1452491"/>
    <dbReference type="KEGG" id="mja:MJ_1582"/>
    <dbReference type="eggNOG" id="arCOG00891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031506_5_2_2"/>
    <dbReference type="InParanoid" id="Q58977"/>
    <dbReference type="OMA" id="PNEAPRI"/>
    <dbReference type="OrthoDB" id="48998at2157"/>
    <dbReference type="PhylomeDB" id="Q58977"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004536">
        <property type="term" value="F:deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015991">
        <property type="entry name" value="Hydrolase_TatD-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00010">
        <property type="entry name" value="TIGR00010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01091">
        <property type="entry name" value="TATD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000202012" description="Uncharacterized metal-dependent hydrolase MJ1582">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0AFQ7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="249" mass="28900" checksum="C83FC884683F0361" modified="1996-11-01" version="1">MRDVKYVDAHCHIEDKAFNKNRDEVIERAKKEDVIIVTSGASLGGCLRALELRKKYNIYLTLGYHPSRVKADDKVIEKVYNLIKNNEYEILAIGEIGMDIKDENYKRQEEIFKKFLSLAEELNKPIVVHARGFERKIFDIAKDKVDIMFHCYSGDVELAKEIGKEGHLISISTLVCFSEHHKKLVESLDLEYLTTETDSPYLSPIKGTKNEPKNVKLVIEEIAKIKEMEVEEVKDVIYKNTCKFFKRRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="140" xmlns="http://uniprot.org/uniprot">
    <accession>Q59007</accession>
    <name>APGM1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1</fullName>
            <shortName>BPG-independent PGAM 1</shortName>
            <shortName>Phosphoglyceromutase 1</shortName>
            <shortName>aPGAM 1</shortName>
            <ecNumber>5.4.2.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>aPGAM-Mj1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">apgM1</name>
        <name type="ordered locus">MJ1612</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="FEBS Lett." volume="517" first="190" last="194">
            <title>A divergent archaeal member of the alkaline phosphatase binuclear metalloenzyme superfamily has phosphoglycerate mutase activity.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="12062435"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(02)02619-4"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="FEMS Microbiol. Lett." volume="212" first="111" last="120">
            <title>Molecular characterization of phosphoglycerate mutase in archaea.</title>
            <authorList>
                <person name="van der Oost J."/>
                <person name="Huynen M.A."/>
                <person name="Verhees C.H."/>
            </authorList>
            <dbReference type="PubMed" id="12076796"/>
            <dbReference type="DOI" id="10.1016/S0378-1097(02)00720-6"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited to approximately 20% by EDTA.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 8.0.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64501">
        <property type="entry name" value="C64501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871137.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59007"/>
    <dbReference type="SMR" id="Q59007"/>
    <dbReference type="STRING" id="243232.MJ_1612"/>
    <dbReference type="DNASU" id="1452521"/>
    <dbReference type="EnsemblBacteria" id="AAB99632">
        <property type="protein sequence ID" value="AAB99632"/>
        <property type="gene ID" value="MJ_1612"/>
    </dbReference>
    <dbReference type="GeneID" id="1452521"/>
    <dbReference type="KEGG" id="mja:MJ_1612"/>
    <dbReference type="eggNOG" id="arCOG01696">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034906_2_0_2"/>
    <dbReference type="InParanoid" id="Q59007"/>
    <dbReference type="OMA" id="IAFRCNF"/>
    <dbReference type="OrthoDB" id="17268at2157"/>
    <dbReference type="PhylomeDB" id="Q59007"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-4905"/>
    <dbReference type="BRENDA" id="5.4.2.12">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046537">
        <property type="term" value="F:2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="PTHR31209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="SSF53649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138134" description="2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1">
        <location>
            <begin position="1"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="12062435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12076796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="12076796"/>
        </source>
    </evidence>
    <sequence length="411" mass="45226" checksum="47A986FD09BA1099" modified="1996-11-01" version="1">MKKGKCVIFIIDGLGDRPNEKGLTPLKEAKTPTMDKIAKEGICGLMNAIDIGIRPGSDTAHLAILGYNPYEVYTGRGPLEAFGVGLDLKEGDIAFRCNFATVDENFVVLDRRAGRISPEEAEELEKEIDGLEIDGVKVIFKSSKGYRGALVLRGEGLSCRVSDGDPHEEGVKVSEIKPLDDSEEAKRTAEILNKLLKIVYEKLNNHPINEERRKKGLPPANIILPRGAGVVPKIEKFSEKYNMKGACICGTGLIKGMAKMIGLDVIEVEGATGTPKTNFMGKAKALVEALKEYDFVLVNVKGADEASHDGNYELKKEVLEKIDEMLAYVFEHINKDEVYFVLTGDHSTPIEMKDHSADPIPIVIWGKSVRVDDVTEFNEFACAKGALHWIKGEHVMKILLDLTGRNEKFGA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q59022</accession>
    <name>Y1628_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1628</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1628</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64503">
        <property type="entry name" value="B64503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871152.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59022"/>
    <dbReference type="STRING" id="243232.MJ_1628"/>
    <dbReference type="EnsemblBacteria" id="AAB99655">
        <property type="protein sequence ID" value="AAB99655"/>
        <property type="gene ID" value="MJ_1628"/>
    </dbReference>
    <dbReference type="GeneID" id="1452537"/>
    <dbReference type="KEGG" id="mja:MJ_1628"/>
    <dbReference type="eggNOG" id="arCOG01746">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_598017_0_0_2"/>
    <dbReference type="InParanoid" id="Q59022"/>
    <dbReference type="OMA" id="MAYQIQR"/>
    <dbReference type="OrthoDB" id="60568at2157"/>
    <dbReference type="PhylomeDB" id="Q59022"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019195">
        <property type="entry name" value="ABC_ATPase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38149">
        <property type="entry name" value="PTHR38149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09818">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107447" description="Uncharacterized protein MJ1628">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="257"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="448" mass="51262" checksum="122A0233DFEFFDA2" modified="1996-11-01" version="1">MFVDEVINNLKGNEFLNTTLLTKSNKNKLYYAVKQPDGNIKVVLPFVFENKNFLKLSEYKDGIEGATQRVIEEIKQEIIKKKRFLPLAGYFGRIYKALYEPLTVVNCNLNLGYDLWKVDKYNYIEGDKIYLMLRMIFKEKDSKEIVKQINELCNDLDKFIKKIPIDLLIDEAKNIINQKYLRDKLDELGLVCFIANNSKPARKYTEVRRHYRIAGPKDVNIPFECPEELEPIEIELKYGKKVKGLGIKKKEIFIITGRNAQGKTTLLQAIDSGRDDHLIGDGREFIITTKSLSKASTGSMEMSGQDISLFFQKLPPGIKGSPKAVYGTASGSMYMAYQIQRAIKNKTKLILIDEDNSAVNLLVSGVLSKWFEGVKSLAEIIMEDREKLGDSSFIIVTSSLDLLTALGDRAIYLEDHKAKYLDLTYFREELGRYYLELASKFIGVKIRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="155" xmlns="http://uniprot.org/uniprot">
    <accession>Q60176</accession>
    <name>MDH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxycarboxylate dehydrogenase (NAD(P)(+))</fullName>
            <ecNumber evidence="2 3 6">1.1.1.375</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">MdhII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mdh</name>
        <name type="synonym">mdhB</name>
        <name type="ordered locus">MJ0490</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="3688" last="3692">
            <title>Identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea.</title>
            <authorList>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10850983"/>
            <dbReference type="DOI" id="10.1128/JB.182.13.3688-3692.2000"/>
        </citation>
        <scope>FUNCTION AS MALATE/SULFOLACTATE DEHYDROGENASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="Mol. Microbiol." volume="37" first="1515" last="1520">
            <title>The putative L-lactate dehydrogenase from Methanococcus jannaschii is an NADPH-dependent L-malate dehydrogenase.</title>
            <authorList>
                <person name="Madern D."/>
            </authorList>
            <dbReference type="PubMed" id="10998181"/>
            <dbReference type="DOI" id="10.1046/j.1365-2958.2000.02113.x"/>
        </citation>
        <scope>FUNCTION AS A MALATE DEHYDROGENASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2001" name="Biochemistry" volume="40" first="10310" last="10316">
            <title>Differences in the oligomeric states of the LDH-like L-MalDH from the hyperthermophilic archaea Methanococcus jannaschii and Archaeoglobus fulgidus.</title>
            <authorList>
                <person name="Madern D."/>
                <person name="Ebel C."/>
                <person name="Dale H.A."/>
                <person name="Lien T."/>
                <person name="Steen I.H."/>
                <person name="Birkeland N.-K."/>
                <person name="Zaccai G."/>
            </authorList>
            <dbReference type="PubMed" id="11513609"/>
            <dbReference type="DOI" id="10.1021/bi010168c"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2009" name="Biochim. Biophys. Acta" volume="1794" first="1496" last="1504">
            <title>Refolding, characterization and crystal structure of (S)-malate dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix.</title>
            <authorList>
                <person name="Kawakami R."/>
                <person name="Sakuraba H."/>
                <person name="Goda S."/>
                <person name="Tsuge H."/>
                <person name="Ohshima T."/>
            </authorList>
            <dbReference type="PubMed" id="19555779"/>
            <dbReference type="DOI" id="10.1016/j.bbapap.2009.06.014"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2001" name="J. Mol. Biol." volume="307" first="1351" last="1362">
            <title>Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases.</title>
            <authorList>
                <person name="Lee B.I."/>
                <person name="Chang C."/>
                <person name="Cho S.-J."/>
                <person name="Eom S.H."/>
                <person name="Kim K.K."/>
                <person name="Yu Y.G."/>
                <person name="Suh S.W."/>
            </authorList>
            <dbReference type="PubMed" id="11292347"/>
            <dbReference type="DOI" id="10.1006/jmbi.2001.4532"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH NADP</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 6">Catalyzes the reversible oxidation of (S)-malate and (S)-sulfolactate to oxaloacetate and sulfopyruvate, respectively. Can use both NADH and NADPH, although activity is higher with NADPH. Oxidation of (S)-sulfolactate is observed only in the presence of NADP(+). Can also oxidize tartrate. Cannot reduce pyruvate, nor alpha-ketoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3 6">
            <text>a (2S)-2-hydroxycarboxylate + NAD(+) = a 2-oxocarboxylate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:34555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.1.375"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3 6">
            <text>a (2S)-2-hydroxycarboxylate + NADP(+) = a 2-oxocarboxylate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:42768"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.375"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.15 mM for (S)-malate (in the presence of NAD(+))</KM>
            <KM evidence="6">0.41 mM for (S)-malate (in the presence of NAD(+))</KM>
            <KM evidence="2">0.025 mM for (S)-malate (in the presence of NADP(+))</KM>
            <KM evidence="6">0.084 mM for (S)-malate (in the presence of NADP(+))</KM>
            <KM evidence="2">4.6 mM for (S)-sulfolactate (in the presence of NADP(+))</KM>
            <KM evidence="6">11 mM for (2S,3S)-tartrate (in the presence of NAD(+))</KM>
            <KM evidence="6">5.1 mM for (2S,3S)-tartrate (in the presence of NADP(+))</KM>
            <KM evidence="2">0.25 mM for oxaloacetate (in the presence of NADH)</KM>
            <KM evidence="2">0.3 mM for oxaloacetate (in the presence of NADPH)</KM>
            <KM evidence="2">1.3 mM for sulfopyruvate (in the presence of NADH)</KM>
            <KM evidence="2">0.19 mM for sulfopyruvate (in the presence of NADPH)</KM>
            <KM evidence="3">0.14 mM for NADH</KM>
            <KM evidence="3">0.02 mM for NADPH</KM>
            <Vmax evidence="2">0.6 umol/min/mg enzyme toward (S)-malate (in the presence of NAD(+))</Vmax>
            <Vmax evidence="2">3.0 umol/min/mg enzyme toward (S)-malate (in the presence of NADP(+))</Vmax>
            <Vmax evidence="2">3.0 umol/min/mg enzyme toward (S)-sulfolactate (in the presence of NADP(+))</Vmax>
            <Vmax evidence="2">29.0 umol/min/mg enzyme toward oxaloacetate (in the presence of NADH)</Vmax>
            <Vmax evidence="2">49.0 umol/min/mg enzyme toward oxaloacetate (in the presence of NADPH)</Vmax>
            <Vmax evidence="2">43.0 umol/min/mg enzyme toward sulfopyruvate (in the presence of NADH)</Vmax>
            <Vmax evidence="2">69.0 umol/min/mg enzyme toward sulfopyruvate (in the presence of NADPH)</Vmax>
            <text evidence="6">kcat is 0.33 sec(-1) for NAD-dependent malate oxidation. kcat is 0.069 sec(-1) for NADP-dependent malate oxidation.</text>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="3 4 5">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.375" evidence="2 3 6"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64361">
        <property type="entry name" value="B64361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869991.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1HYE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-313"/>
    </dbReference>
    <dbReference type="PDB" id="1HYG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-313"/>
    </dbReference>
    <dbReference type="PDBsum" id="1HYE"/>
    <dbReference type="PDBsum" id="1HYG"/>
    <dbReference type="AlphaFoldDB" id="Q60176"/>
    <dbReference type="SMR" id="Q60176"/>
    <dbReference type="STRING" id="243232.MJ_0490"/>
    <dbReference type="EnsemblBacteria" id="AAB98481">
        <property type="protein sequence ID" value="AAB98481"/>
        <property type="gene ID" value="MJ_0490"/>
    </dbReference>
    <dbReference type="GeneID" id="1451352"/>
    <dbReference type="KEGG" id="mja:MJ_0490"/>
    <dbReference type="eggNOG" id="arCOG00246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045401_2_2_2"/>
    <dbReference type="InParanoid" id="Q60176"/>
    <dbReference type="OMA" id="ASCAEYI"/>
    <dbReference type="OrthoDB" id="69007at2157"/>
    <dbReference type="PhylomeDB" id="Q60176"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-18778"/>
    <dbReference type="BRENDA" id="1.1.1.375">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q60176"/>
    <dbReference type="EvolutionaryTrace" id="Q60176"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102443">
        <property type="term" value="F:L-2-hydroxycarboxylate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018177">
        <property type="entry name" value="L-lactate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="SSF56327"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000113485" description="L-2-hydroxycarboxylate dehydrogenase (NAD(P)(+))">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="7"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="2"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="7"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="28"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="60"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="69"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="76"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="125"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="149"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="167"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="174"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="182"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="186"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="200"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="219"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="225"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="249"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="265"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="279"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P61889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10850983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="10998181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="11292347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="11513609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="19555779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="7">
        <source>
            <dbReference type="PubMed" id="10850983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8"/>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="1HYE"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="1HYG"/>
        </source>
    </evidence>
    <sequence length="313" mass="34609" checksum="A7C0DB67C50151CC" modified="1997-11-01" version="1">MKVTIIGASGRVGSATALLLAKEPFMKDLVLIGREHSINKLEGLREDIYDALAGTRSDANIYVESDENLRIIDESDVVIITSGVPRKEGMSRMDLAKTNAKIVGKYAKKIAEICDTKIFVITNPVDVMTYKALVDSKFERNQVFGLGTHLDSLRFKVAIAKFFGVHIDEVRTRIIGEHGDSMVPLLSATSIGGIPIQKFERFKELPIDEIIEDVKTKGEQIIRLKGGSEFGPAAAILNVVRCIVNNEKRLLTLSAYVDGEFDGIRDVCIGVPVKIGRDGIEEVVSIELDKDEIIAFRKSAEIIKKYCEEVKNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q60279</accession>
    <name>Y3519_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL19</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64512">
        <property type="entry name" value="B64512"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890066.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60279"/>
    <dbReference type="SMR" id="Q60279"/>
    <dbReference type="STRING" id="243232.MJ_ECL19"/>
    <dbReference type="EnsemblBacteria" id="AAC37090">
        <property type="protein sequence ID" value="AAC37090"/>
        <property type="gene ID" value="MJ_ECL19"/>
    </dbReference>
    <dbReference type="GeneID" id="1450803"/>
    <dbReference type="KEGG" id="mja:MJ_ECL19"/>
    <dbReference type="eggNOG" id="arCOG03827">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1536670_0_0_2"/>
    <dbReference type="InParanoid" id="Q60279"/>
    <dbReference type="PhylomeDB" id="Q60279"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107507" description="Uncharacterized protein MJECL19">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <sequence length="174" mass="20273" checksum="D1082FF3171527E4" modified="1997-11-01" version="1">MESKEFDDDKIKIISMTPKSEKSDKIVMQIVEEFINSFPDDRYKFRVLLKVAELICKNGLCNEAFLILDKIPDSYYKSSALYKMADILYRNKEHDRLIQIAEKIPDDYKKSEVLLKVVELLCESGKYDEAINIAEKIPDNYYKSEALFKIAETLSNKGYYDKAVEIAEKIPDNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q60288</accession>
    <name>VAPC6_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease VapC6</fullName>
            <shortName evidence="1">RNase VapC6</shortName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Putative toxin VapC6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">vapC6</name>
        <name type="ordered locus">MJECL30</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. An RNase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64513">
        <property type="entry name" value="E64513"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890077.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60288"/>
    <dbReference type="SMR" id="Q60288"/>
    <dbReference type="STRING" id="243232.MJ_ECL30"/>
    <dbReference type="EnsemblBacteria" id="AAC37100">
        <property type="protein sequence ID" value="AAC37100"/>
        <property type="gene ID" value="MJ_ECL30"/>
    </dbReference>
    <dbReference type="GeneID" id="1450813"/>
    <dbReference type="KEGG" id="mja:MJ_ECL30"/>
    <dbReference type="eggNOG" id="arCOG00710">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134210_1_1_2"/>
    <dbReference type="InParanoid" id="Q60288"/>
    <dbReference type="OMA" id="DRYAWVY"/>
    <dbReference type="OrthoDB" id="88061at2157"/>
    <dbReference type="PhylomeDB" id="Q60288"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107515" description="Ribonuclease VapC6">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="6"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <sequence length="153" mass="17845" checksum="BDD035D67F615755" modified="1997-11-01" version="1">MSPSNVFIDSSVMVGLFIGDEKAHKLLSKLINDGFMLCINPIVFSETMFKVTFHIALEDGIRGVYDLKKNLNRYSWVYEEVREKIDKMIKMNYLKILDTNWEVLKLAPEIGKKYNLLTNDAIIIATCKYYRINKLATFDSDFEKVDFIEIIKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q60295</accession>
    <name>T1RH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative type I restriction enzyme MjaIXP endonuclease subunit</fullName>
            <shortName evidence="4">MjaIXP</shortName>
            <shortName evidence="5">R protein</shortName>
            <ecNumber evidence="1">3.1.21.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative type-1 restriction enzyme MjaXP R protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL40</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 4">The restriction (R) subunit of a type I restriction enzyme that recognizes 5'-CCAN(5)GTR-3' and cleaves a random distance away. The R subunit is required for both nuclease and ATPase activities, but not for modification. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HsdR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.3" evidence="1"/>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64514">
        <property type="entry name" value="G64514"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60295"/>
    <dbReference type="SMR" id="Q60295"/>
    <dbReference type="STRING" id="243232.MJ_ECL40"/>
    <dbReference type="REBASE" id="191891">
        <property type="entry name" value="Apa1468ORF2715P"/>
    </dbReference>
    <dbReference type="REBASE" id="3908">
        <property type="entry name" value="MjaIXP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAC37109">
        <property type="protein sequence ID" value="AAC37109"/>
        <property type="gene ID" value="MJ_ECL40"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_ECL40"/>
    <dbReference type="eggNOG" id="arCOG00878">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005762_1_0_2"/>
    <dbReference type="InParanoid" id="Q60295"/>
    <dbReference type="OMA" id="KCARFHQ"/>
    <dbReference type="PhylomeDB" id="Q60295"/>
    <dbReference type="PRO" id="PR:Q60295"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007409">
        <property type="entry name" value="Restrct_endonuc_type1_HsdR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004473">
        <property type="entry name" value="Restrct_endonuc_typeI_HsdR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040980">
        <property type="entry name" value="SWI2_SNF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021810">
        <property type="entry name" value="T1RH-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11867">
        <property type="entry name" value="DUF3387"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04313">
        <property type="entry name" value="HSDR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18766">
        <property type="entry name" value="SWI2_SNF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00348">
        <property type="entry name" value="hsdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000077273" description="Putative type I restriction enzyme MjaIXP endonuclease subunit">
        <location>
            <begin position="1"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="323"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="3">
        <location>
            <begin position="551"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box" evidence="2">
        <location>
            <begin position="439"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P08956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="1042" mass="121523" checksum="3C8E5A103E7B59D3" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q60296</accession>
    <name>T1SH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Type I restriction enzyme MjaIX specificity subunit</fullName>
            <shortName>S protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Type I specificity subunit S.MjaIX</fullName>
            <shortName evidence="2">S.MjaIX</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Type-1 restriction enzyme MjaXP specificity subunit</fullName>
            <shortName>S.MjaXP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hsdS</name>
        <name type="ordered locus">MJECL41</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">The specificity (S) subunit of a type I restriction enzyme; this subunit dictates DNA sequence specificity. The M and S subunits together form a methyltransferase (MTase) that methylates A-3 on the top and A-2 on the bottom strand of the sequence 5'-CCAN(5)GTR-3'. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg(2+) as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64514">
        <property type="entry name" value="H64514"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60296"/>
    <dbReference type="SMR" id="Q60296"/>
    <dbReference type="STRING" id="243232.MJ_ECL41"/>
    <dbReference type="REBASE" id="191890">
        <property type="entry name" value="S2.Apa1468ORF3072P"/>
    </dbReference>
    <dbReference type="REBASE" id="3909">
        <property type="entry name" value="S.MjaIX"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAC37110">
        <property type="protein sequence ID" value="AAC37110"/>
        <property type="gene ID" value="MJ_ECL41"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_ECL41"/>
    <dbReference type="eggNOG" id="arCOG02628">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021095_2_3_2"/>
    <dbReference type="InParanoid" id="Q60296"/>
    <dbReference type="OMA" id="KRWFVDF"/>
    <dbReference type="PhylomeDB" id="Q60296"/>
    <dbReference type="PRO" id="PR:Q60296"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000198053" description="Type I restriction enzyme MjaIX specificity subunit">
        <location>
            <begin position="1"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P05719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="432" mass="50160" checksum="84CD44CF20EBC42C" modified="1996-11-01" version="1">MLFMVKFRWETEFKETDIGKIPKDWDVKKIKDIGEVAGGSTPSTKIKEYWGGDIPWITPKDLANYEYIYISRGERNITEKAVKECSLRIFPKGTILLTSRAPIGYVAIAKNPLTTNQGFRNIIPKDGVVSEYLYYLFKTKTMSEYLKDISGGSTFPELKGSTLKEVEIPYPSPEEQQKIATVLSYFDDLIENKKKQNEILEKIALELFKNWFIDFEPFKNEEFVYNDELDKEIPKGWEVKRLGDILKVESGSNAPQREIYFENAKIPFVRVKHLVKGVCIESSDFINELALKDYKMKLYNEKSIIFQKSGESLKEARVNIVPFKFTAVNHLAVIDSSMLNEKHYFIYCLLRFLLKEIVYSVKGTTLPYLKISDIENKYIIIPPQPILQKFHSLVQPLFEKIINNQKQIMVLKKIRDALLPKLVFGELRVEEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q60345</accession>
    <name>Y038_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0038</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0038</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64304">
        <property type="entry name" value="F64304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869529.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60345"/>
    <dbReference type="SMR" id="Q60345"/>
    <dbReference type="STRING" id="243232.MJ_0038"/>
    <dbReference type="EnsemblBacteria" id="AAB98019">
        <property type="protein sequence ID" value="AAB98019"/>
        <property type="gene ID" value="MJ_0038"/>
    </dbReference>
    <dbReference type="GeneID" id="1450876"/>
    <dbReference type="KEGG" id="mja:MJ_0038"/>
    <dbReference type="eggNOG" id="arCOG04130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076814_1_0_2"/>
    <dbReference type="InParanoid" id="Q60345"/>
    <dbReference type="OMA" id="IGKKLMW"/>
    <dbReference type="OrthoDB" id="112466at2157"/>
    <dbReference type="PhylomeDB" id="Q60345"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="PTHR40734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106662" description="Uncharacterized protein MJ0038">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <sequence length="217" mass="26274" checksum="00C21A7773BD9813" modified="1997-11-01" version="1">MVRGQYKKGNDERMRFPKKNKPQKFENYAWVLDYLPYGYPDKPDEPIVQGLGEYQFLLMEMIPKPNVDIELGERVYIGKGKRDKIDHVRRMIKYEQLTPTAKSELLYVVMEAVKIQEDRFVRFFNECPPITTRLHTLELLPEIKKKYMWKIIEEREAKKFESFKDFEERIGKNPVRIIAKRIEKELSDDKKDKYYLFVKWKKGIILNEDNMTFYLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q60347</accession>
    <name>Y042_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0042</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0042</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64305">
        <property type="entry name" value="B64305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869533.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60347"/>
    <dbReference type="STRING" id="243232.MJ_0042"/>
    <dbReference type="EnsemblBacteria" id="AAB98028">
        <property type="protein sequence ID" value="AAB98028"/>
        <property type="gene ID" value="MJ_0042"/>
    </dbReference>
    <dbReference type="GeneID" id="1450880"/>
    <dbReference type="KEGG" id="mja:MJ_0042"/>
    <dbReference type="HOGENOM" id="CLU_1280805_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011723">
        <property type="entry name" value="Znf/thioredoxin_put"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02098">
        <property type="entry name" value="MJ0042_CXXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106665" description="Uncharacterized protein MJ0042">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <sequence length="215" mass="24892" checksum="D1F39FD383A3A0A1" modified="1997-11-01" version="1">MNVKCPECGAWIYVVEEDSGGDAMEVKCPKCGTSIYVVKPMGEKMKNKRDKDFLDVKILEIEETKKTSPYKDTKSEDVLKALRVKANINGEIYEFRIWQIAKKPEYRGMVYVVKSVSHYCGSVKTKNFQVDEDNDIYVKQKFGIIEGVNKSKIKLPKERMEEIAEKLGFELKEGDEGLRLYLGEKYSENPPLSQRPELIEKLIKCWIAFWEPTMI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q60363</accession>
    <name>PTH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-tRNA hydrolase</fullName>
            <shortName evidence="1">PTH</shortName>
            <ecNumber evidence="1">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pth</name>
        <name type="synonym">pth2</name>
        <name type="ordered locus">MJ0051</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Proc. Natl. Acad. Sci. U.S.A." volume="99" first="16707" last="16712">
            <title>Orthologs of a novel archaeal and of the bacterial peptidyl-tRNA hydrolase are nonessential in yeast.</title>
            <authorList>
                <person name="Rosas-Sandoval G."/>
                <person name="Ambrogelly A."/>
                <person name="Rinehart J."/>
                <person name="Wei D."/>
                <person name="Cruz-Vera L.R."/>
                <person name="Graham D.E."/>
                <person name="Stetter K.O."/>
                <person name="Guarneros G."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="12475929"/>
            <dbReference type="DOI" id="10.1073/pnas.222659199"/>
        </citation>
        <scope>PARTIAL PROTEIN SEQUENCE</scope>
        <scope>FUNCTION</scope>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PTH2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64306">
        <property type="entry name" value="C64306"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869543.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2ZV3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I=1-115"/>
    </dbReference>
    <dbReference type="PDBsum" id="2ZV3"/>
    <dbReference type="AlphaFoldDB" id="Q60363"/>
    <dbReference type="SMR" id="Q60363"/>
    <dbReference type="STRING" id="243232.MJ_0051"/>
    <dbReference type="EnsemblBacteria" id="AAB98032">
        <property type="protein sequence ID" value="AAB98032"/>
        <property type="gene ID" value="MJ_0051"/>
    </dbReference>
    <dbReference type="GeneID" id="1450890"/>
    <dbReference type="KEGG" id="mja:MJ_0051"/>
    <dbReference type="eggNOG" id="arCOG04228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073661_2_2_2"/>
    <dbReference type="InParanoid" id="Q60363"/>
    <dbReference type="OMA" id="GHAAVEC"/>
    <dbReference type="OrthoDB" id="115947at2157"/>
    <dbReference type="PhylomeDB" id="Q60363"/>
    <dbReference type="BRENDA" id="3.1.1.29">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q60363"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:aminoacyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00628">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034759">
        <property type="entry name" value="Pept_tRNA_hydro_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PTHR12649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="SSF102462"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000120292" description="Peptidyl-tRNA hydrolase">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="15"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="38"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="51"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="76"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="102"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12475929"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2ZV3"/>
        </source>
    </evidence>
    <sequence length="115" mass="12670" checksum="FFBC33D185B4A8A2" modified="1997-11-01" version="1">MKMVVVIRNDLGMGKGKMVAQGGHAIIEAFLDAKRKNPRAVDEWLREGQKKVVVKVNSEKELIDIYNKARSEGLPCSIIRDAGHTQLEPGTLTAVAIGPEKDEKIDKITGHLKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q60376</accession>
    <name>Y064_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0064</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0064</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64307">
        <property type="entry name" value="H64307"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60376"/>
    <dbReference type="SMR" id="Q60376"/>
    <dbReference type="STRING" id="243232.MJ_0064"/>
    <dbReference type="PRIDE" id="Q60376"/>
    <dbReference type="EnsemblBacteria" id="AAB98051">
        <property type="protein sequence ID" value="AAB98051"/>
        <property type="gene ID" value="MJ_0064"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0064"/>
    <dbReference type="eggNOG" id="arCOG03876">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444673_0_0_2"/>
    <dbReference type="OMA" id="EIHYGLK"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106675" description="Uncharacterized protein MJ0064">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <sequence length="189" mass="21635" checksum="BC45A1FC9AD8DB58" modified="1997-11-01" version="1">MIKMNDKQADWLNKLIEELDKSSSSVLETEIVLDEIITKLNNLISEINNYITEIHYGLRITNERINLSVSELDELEKKLKEYLDFAENMKKSVENTHNAIIAVKNEMRDIKHSLEESSLKISNRYNLMEDTLKHHSKKIESLSNSIKSLSKTQESIINSINSTKMLLYITIALTVVNLIISLKTSGIIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-04-05" modified="2022-05-25" version="22" xmlns="http://uniprot.org/uniprot">
    <accession>P0CL56</accession>
    <name>RELB3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Antitoxin RelB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">relB3</name>
        <name type="synonym">relB</name>
        <name type="ordered locus">MJ1103.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>IDENTIFICATION</scope>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="J. Mol. Biol." volume="393" first="898" last="908">
            <title>Crystal structure of the antitoxin-toxin protein complex RelB-RelE from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Francuski D."/>
                <person name="Saenger W."/>
            </authorList>
            <dbReference type="PubMed" id="19712680"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2009.08.048"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-52</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Antitoxin component of a type II toxin-antitoxin (TA) system. Probably neutralizes the toxic activity of cognate toxin RelE.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms heterodimers with RelE and possibly a heterotetramer RelE3-RelB3(2)-RelE3 from 2 heterodimers. The heterotetramer is probably not very stable in solution.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="3BPQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/C=1-52"/>
    </dbReference>
    <dbReference type="PDBsum" id="3BPQ"/>
    <dbReference type="AlphaFoldDB" id="P0CL56"/>
    <dbReference type="SMR" id="P0CL56"/>
    <dbReference type="EvolutionaryTrace" id="P0CL56"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000406203" description="Antitoxin RelB3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="43"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19712680"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3BPQ"/>
        </source>
    </evidence>
    <sequence length="53" mass="6440" checksum="6F309BD5CBD5CF5F" modified="2011-04-05" version="1">MRLKKRFKKFFISRKEYEKIEEILDIGLAKAMEETKDDELLTYDEIKELLGDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P54011</accession>
    <name>RL37_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L37e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl37e</name>
        <name type="ordered locus">MJ0098</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64312">
        <property type="entry name" value="B64312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869590.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54011"/>
    <dbReference type="SMR" id="P54011"/>
    <dbReference type="STRING" id="243232.MJ_0098"/>
    <dbReference type="EnsemblBacteria" id="AAB98078">
        <property type="protein sequence ID" value="AAB98078"/>
        <property type="gene ID" value="MJ_0098"/>
    </dbReference>
    <dbReference type="GeneID" id="1450937"/>
    <dbReference type="KEGG" id="mja:MJ_0098"/>
    <dbReference type="eggNOG" id="arCOG04126">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_208825_0_0_2"/>
    <dbReference type="InParanoid" id="P54011"/>
    <dbReference type="OMA" id="RIRRYSW"/>
    <dbReference type="OrthoDB" id="124429at2157"/>
    <dbReference type="PhylomeDB" id="P54011"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_L37ae/L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_L37e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_L37e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000139729" description="50S ribosomal protein L37e">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="61" mass="7258" checksum="435283F8858B20CE" modified="1996-10-01" version="1">MSKGTPSMGKRNKGSYHIRCRRCGRRAYHVRKKRCAACGFPNKRMRKYSWQNKKVNGKRIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>P54028</accession>
    <name>RS27_METJA</name>
    <protein>
        <recommendedName>
            <fullName>30S ribosomal protein S27e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rps27e</name>
        <name type="ordered locus">MJ0250</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64331">
        <property type="entry name" value="C64331"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54028"/>
    <dbReference type="SMR" id="P54028"/>
    <dbReference type="STRING" id="243232.MJ_0250"/>
    <dbReference type="EnsemblBacteria" id="AAB98237">
        <property type="protein sequence ID" value="AAB98237"/>
        <property type="gene ID" value="MJ_0250"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0250"/>
    <dbReference type="eggNOG" id="arCOG04108">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199465_0_0_2"/>
    <dbReference type="InParanoid" id="P54028"/>
    <dbReference type="OMA" id="DCENEQV"/>
    <dbReference type="PhylomeDB" id="P54028"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_S27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_S27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000149072" description="30S ribosomal protein S27e">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="62" mass="6797" checksum="0BD2AD7F93157BCB" modified="1996-10-01" version="1">MMELIPQPRTKFLRVQCPECNNEQIVFGSPATVVKCLTCGKVLVEPRGGKGKVKAKILEILG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>P54043</accession>
    <name>RL19E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl19e</name>
        <name type="ordered locus">MJ0473</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64359">
        <property type="entry name" value="A64359"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54043"/>
    <dbReference type="SMR" id="P54043"/>
    <dbReference type="STRING" id="243232.MJ_0473"/>
    <dbReference type="EnsemblBacteria" id="AAB98462">
        <property type="protein sequence ID" value="AAB98462"/>
        <property type="gene ID" value="MJ_0473"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0473"/>
    <dbReference type="eggNOG" id="arCOG04089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_1_1_2"/>
    <dbReference type="InParanoid" id="P54043"/>
    <dbReference type="OMA" id="NRVWIDP"/>
    <dbReference type="PhylomeDB" id="P54043"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01418">
        <property type="entry name" value="Ribosomal_L19e_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_L19/L19e_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_L19/L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_L19/L19e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_L19/L19e_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033936">
        <property type="entry name" value="Ribosomal_L19e_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="RPL19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="PTHR10722"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="SSF48140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131189" description="50S ribosomal protein L19e">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="57"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="151" mass="17630" checksum="BFB46E99A0520FF0" modified="1996-10-01" version="1">MIIMDVSVQRRMAAEILKCGIERVWIDPTQLDRVKMAMSKDDIRALIKEGVIKKKQKKGISSARVKKLKEQRKKGRRRGPGSRRGAAGARTPPKERWMATIRALRKTLKQLRDSGKIDRKVYRKLYRMAKGGAFRSRSHLFLYMREHELLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>P54057</accession>
    <name>RS19E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps19e</name>
        <name type="ordered locus">MJ0692</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64386">
        <property type="entry name" value="D64386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870197.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54057"/>
    <dbReference type="SMR" id="P54057"/>
    <dbReference type="STRING" id="243232.MJ_0692"/>
    <dbReference type="EnsemblBacteria" id="AAB98687">
        <property type="protein sequence ID" value="AAB98687"/>
        <property type="gene ID" value="MJ_0692"/>
    </dbReference>
    <dbReference type="GeneID" id="1451558"/>
    <dbReference type="KEGG" id="mja:MJ_0692"/>
    <dbReference type="eggNOG" id="arCOG01344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_1_0_2"/>
    <dbReference type="InParanoid" id="P54057"/>
    <dbReference type="OMA" id="YIDGPVG"/>
    <dbReference type="OrthoDB" id="92409at2157"/>
    <dbReference type="PhylomeDB" id="P54057"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_S19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_S19e_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_S19e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="PTHR11710"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153840" description="30S ribosomal protein S19e">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <sequence length="148" mass="17030" checksum="CEE6467899CF89BE" modified="1996-10-01" version="1">MVTVYDVPADKLIQKTAEKLKEMNIGVPEWVDFVKTGVSRERRPDQDDWWYIRCASILRKIYIYGPVGVSRLRTAYGGRKNRGHEPEHFYKGSGNIIRKALQELEKLGLVEKTPEGRVVTPKGRSFLDNIAKEVRDEIINEIPALAKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>P54066</accession>
    <name>RL7A_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L7Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein L8e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl7ae</name>
        <name type="ordered locus">MJ1203</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Structure" volume="12" first="893" last="903">
            <title>Structure of protein L7Ae bound to a K-turn derived from an archaeal box H/ACA sRNA at 1.8 A resolution.</title>
            <authorList>
                <person name="Hamma T."/>
                <person name="Ferre-D'Amare A.R."/>
            </authorList>
            <dbReference type="PubMed" id="15130481"/>
            <dbReference type="DOI" id="10.1016/j.str.2004.03.015"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS)</scope>
        <scope>RNA-BINDING</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Biochemistry" volume="44" first="9657" last="9672">
            <title>The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs.</title>
            <authorList>
                <person name="Suryadi J."/>
                <person name="Tran E.J."/>
                <person name="Maxwell E.S."/>
                <person name="Brown B.A. II"/>
            </authorList>
            <dbReference type="PubMed" id="16008351"/>
            <dbReference type="DOI" id="10.1021/bi050568q"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text>Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-2944269">
            <id>P54066</id>
        </interactant>
        <interactant intactId="EBI-2944259">
            <id>Q58105</id>
            <label>MJ0694</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>4</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870715.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1RA4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.86 A"/>
        <property type="chains" value="A=1-117"/>
    </dbReference>
    <dbReference type="PDB" id="1SDS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B/C=1-117"/>
    </dbReference>
    <dbReference type="PDB" id="1XBI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.45 A"/>
        <property type="chains" value="A=1-117"/>
    </dbReference>
    <dbReference type="PDB" id="3PAF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B=1-117"/>
    </dbReference>
    <dbReference type="PDB" id="6K0A">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.60 A"/>
        <property type="chains" value="I/J=1-117"/>
    </dbReference>
    <dbReference type="PDB" id="6K0B">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.30 A"/>
        <property type="chains" value="I/J=1-117"/>
    </dbReference>
    <dbReference type="PDBsum" id="1RA4"/>
    <dbReference type="PDBsum" id="1SDS"/>
    <dbReference type="PDBsum" id="1XBI"/>
    <dbReference type="PDBsum" id="3PAF"/>
    <dbReference type="PDBsum" id="6K0A"/>
    <dbReference type="PDBsum" id="6K0B"/>
    <dbReference type="AlphaFoldDB" id="P54066"/>
    <dbReference type="SMR" id="P54066"/>
    <dbReference type="IntAct" id="P54066">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1203"/>
    <dbReference type="MoonProt" id="P54066"/>
    <dbReference type="EnsemblBacteria" id="AAB99207">
        <property type="protein sequence ID" value="AAB99207"/>
        <property type="gene ID" value="MJ_1203"/>
    </dbReference>
    <dbReference type="GeneID" id="1452098"/>
    <dbReference type="KEGG" id="mja:MJ_1203"/>
    <dbReference type="eggNOG" id="arCOG01751">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084513_4_0_2"/>
    <dbReference type="InParanoid" id="P54066"/>
    <dbReference type="OMA" id="KPFYVRF"/>
    <dbReference type="OrthoDB" id="114541at2157"/>
    <dbReference type="PhylomeDB" id="P54066"/>
    <dbReference type="EvolutionaryTrace" id="P54066"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D RNP complex"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034512">
        <property type="term" value="F:box C/D RNA binding"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_L7Ae_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_L7Ae/L8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_L7Ae_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_L7Ae_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000948">
        <property type="entry name" value="Ribosomal_L7Ae_prok"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000136793" description="50S ribosomal protein L7Ae">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="25"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="31"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="44"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="61"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="72"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="78"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="92"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="102"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="1XBI"/>
        </source>
    </evidence>
    <sequence length="117" mass="12686" checksum="7CAB706F09371BA6" modified="1998-07-15" version="2">MAVYVKFKVPEEIQKELLDAVAKAQKIKKGANEVTKAVERGIAKLVIIAEDVKPEEVVAHLPYLCEEKGIPYAYVASKQDLGKAAGLEVAASSVAIINEGDAEELKVLIEKVNVLKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P54067</accession>
    <name>RS6E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S6e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps6e</name>
        <name type="ordered locus">MJ1260</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64457">
        <property type="entry name" value="C64457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870773.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54067"/>
    <dbReference type="SMR" id="P54067"/>
    <dbReference type="STRING" id="243232.MJ_1260"/>
    <dbReference type="EnsemblBacteria" id="AAB99263">
        <property type="protein sequence ID" value="AAB99263"/>
        <property type="gene ID" value="MJ_1260"/>
    </dbReference>
    <dbReference type="GeneID" id="1452158"/>
    <dbReference type="KEGG" id="mja:MJ_1260"/>
    <dbReference type="eggNOG" id="arCOG01946">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109671_1_1_2"/>
    <dbReference type="InParanoid" id="P54067"/>
    <dbReference type="OMA" id="DGFPMRP"/>
    <dbReference type="OrthoDB" id="112720at2157"/>
    <dbReference type="PhylomeDB" id="P54067"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_S6e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_S6e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_S6e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="PTHR11502"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000137348" description="30S ribosomal protein S6e">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="131" mass="14372" checksum="6BFD365C1BFF006D" modified="1996-10-01" version="1">MPTAKFVVADPKTGRCYQIEADNTPLVGKKIGEVFDGKIIGLEGYKLQIRGGTDSSGFPMRPDIHGSRKVRVLLSAPPGFKPKRKGERRRKTVRGNTIAPDIVQINVKVVEYGEKSIPELLGLEGGENQEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>P60200</accession>
    <name>HDRA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CoB--CoM heterodisulfide reductase iron-sulfur subunit A</fullName>
            <ecNumber evidence="5">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrA</name>
        <name type="ordered locus">MJ1190</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="J. Mol. Biol." volume="266" first="637" last="641">
            <title>Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion.</title>
            <authorList>
                <person name="Wilting R."/>
                <person name="Schorling S."/>
                <person name="Persson B.C."/>
                <person name="Boeck A."/>
            </authorList>
            <dbReference type="PubMed" id="9102456"/>
            <dbReference type="DOI" id="10.1006/jmbi.1996.0812"/>
        </citation>
        <scope>PROBABLE SELENOCYSTEINE AT SEC-196</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 4 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The ferredoxin:CoB-CoM heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HdrA family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="5"/>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="P60200"/>
    <dbReference type="OMA" id="CPSGAMQ"/>
    <dbReference type="PhylomeDB" id="P60200"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039650">
        <property type="entry name" value="HdrA-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43498">
        <property type="entry name" value="PTHR43498"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <feature type="chain" id="PRO_0000150057" description="CoB--CoM heterodisulfide reductase iron-sulfur subunit A">
        <location>
            <begin position="1"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="4">
        <location>
            <begin position="235"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="4">
        <location>
            <begin position="283"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 3" evidence="4">
        <location>
            <begin position="574"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 4" evidence="4">
        <location>
            <begin position="607"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="586"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="589"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="593"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="616"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="619"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="622"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="626"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="5">
        <location>
            <position position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LWL2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8TM02"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="657" mass="71922" checksum="D3607042434DBC32" modified="2008-02-26" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>P81230</accession>
    <name>YC4B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1249.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1249.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0795.1 and MJ0785.1.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81230"/>
    <dbReference type="SMR" id="P81230"/>
    <dbReference type="STRING" id="243232.MJ_1249.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99259">
        <property type="protein sequence ID" value="AAB99259"/>
        <property type="gene ID" value="MJ_1249.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1249.1"/>
    <dbReference type="eggNOG" id="arCOG09674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451435_0_0_2"/>
    <dbReference type="BRENDA" id="1.4.1.24">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107240" description="Uncharacterized protein MJ1249.1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="166" mass="19749" checksum="FEA9B13E58F3A725" modified="1998-07-15" version="1">MRGINPVLFKISFLLIILVSLILSLFYYNFLFAFLLSFILFGITWAYCYIKIESTDKGKLLYEIKRPGIETLRFLFILMIISVFIKSLLHSNSFFPYISFLLSNLILGLVLFDDYILGNPTIKFYEKGVVFDRVAFYNWEELDIKEDEGYLKIKIKYYPKEIMYKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>P81324</accession>
    <name>Y83C_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0835.2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0835.2</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.thermoautotrophicum MTH421.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870349.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81324"/>
    <dbReference type="STRING" id="243232.MJ_0835.2"/>
    <dbReference type="MEROPS" id="A24.018"/>
    <dbReference type="EnsemblBacteria" id="AAB98835">
        <property type="protein sequence ID" value="AAB98835"/>
        <property type="gene ID" value="MJ_0835.2"/>
    </dbReference>
    <dbReference type="GeneID" id="1451721"/>
    <dbReference type="KEGG" id="mja:MJ_0835.2"/>
    <dbReference type="eggNOG" id="arCOG02300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_805645_0_0_2"/>
    <dbReference type="InParanoid" id="P81324"/>
    <dbReference type="OMA" id="WKDIIPS"/>
    <dbReference type="OrthoDB" id="49068at2157"/>
    <dbReference type="PhylomeDB" id="P81324"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009639">
        <property type="entry name" value="Pept_A24A_C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06819">
        <property type="entry name" value="Arc_PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107071" description="Uncharacterized protein MJ0835.2">
        <location>
            <begin position="1"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="204"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="339" mass="39339" checksum="7A8C60062CEF5BFA" modified="1998-07-15" version="1">MDLLNVVYIINFILLLLAAITDIKERIIPHKYTIAMIIINLVVGYYYFGFNAIIAFFSTLILCLILSIGMGGGDVKLFTALAPIFAYPNSFVFYIPKYILYLIAISMFIAAVFPMYKILMRYWKDIIPSACYLTMMLGILYYFINIYEIPYASIIIWAYIVLSIFVSRKVPKYKEYTKKLGYLFPAYLLFLYIIDTTYFIKYNVLLTSIIYLCEIILISIVIYALTGVETSDKKHIEELKEGDILRDVIIIDKDGVEVKNLNIMKRIKFLLEHEIKENEKEIILTDGEGLSNEDIRKIKKLYMEGKIPDKLNVIKTYPFVPFVVIGYVIVLMLMKLAII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q49593</accession>
    <accession>Q58279</accession>
    <name>RADA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">radA</name>
        <name type="ordered locus">MJ0869</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Nucleic Acids Res." volume="24" first="2125" last="2132">
            <title>recA-like genes from three archaean species with putative protein products similar to Rad51 and Dmc1 proteins of the yeast Saccharomyces cerevisiae.</title>
            <authorList>
                <person name="Sandler S.J."/>
                <person name="Satin L.H."/>
                <person name="Samra H.S."/>
                <person name="Clark A.J."/>
            </authorList>
            <dbReference type="PubMed" id="8668545"/>
            <dbReference type="DOI" id="10.1093/nar/24.11.2125"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <dbReference type="EMBL" id="U45312">
        <property type="protein sequence ID" value="AAC44122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64408">
        <property type="entry name" value="E64408"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q49593"/>
    <dbReference type="SMR" id="Q49593"/>
    <dbReference type="STRING" id="243232.MJ_0869"/>
    <dbReference type="EnsemblBacteria" id="AAB98875">
        <property type="protein sequence ID" value="AAB98875"/>
        <property type="gene ID" value="MJ_0869"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0869"/>
    <dbReference type="eggNOG" id="arCOG00415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041732_0_0_2"/>
    <dbReference type="InParanoid" id="Q49593"/>
    <dbReference type="OMA" id="TFRIYLR"/>
    <dbReference type="PhylomeDB" id="Q49593"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01123">
        <property type="entry name" value="Rad51_DMC1_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00348">
        <property type="entry name" value="RadA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="DNA_recomb/repair_Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011938">
        <property type="entry name" value="DNA_recomb/repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033925">
        <property type="entry name" value="Rad51_DMC1_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005856">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="SSF47794"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02236">
        <property type="entry name" value="recomb_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50163">
        <property type="entry name" value="RECA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150095" description="DNA repair and recombination protein RadA">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="136"/>
            <end position="143"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="352" mass="39084" checksum="861579C0C59AA29C" modified="1997-11-01" version="1">MITFIYFFGNIIYYLPIYSYINNYKFMLMVIIMDDLTQLPGVGPTTAEKLKEAGYTDFMKIATASIGELTEIDGISEKAAARIIEAARELCNLGFKSGTEVLSQRKNIWKLSTGSKNLDEILGGGLESQSVTEFAGMFGSGKTQIAHQACVNLQCPERIVADDAIKDEILNEPKAVYIDTEGTFRPERIVQMAEALGLDGNEVLNNIFVARAYNSDMQMLYAENVENLIREGHNIKLVIVDSLTSTFRTEYIGRGKLAERQQKLGRHMATLNKLADIYNCVVIVTNQVAARPDALFGPSEQAIGGHIVGHAATFRIFLRKAKGDKRVAKLYDSPHLPDAEAMFRITEKGIHD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57551</accession>
    <name>Y086_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0086</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0086</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64310">
        <property type="entry name" value="F64310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869578.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57551"/>
    <dbReference type="SMR" id="Q57551"/>
    <dbReference type="STRING" id="243232.MJ_0086"/>
    <dbReference type="EnsemblBacteria" id="AAB98068">
        <property type="protein sequence ID" value="AAB98068"/>
        <property type="gene ID" value="MJ_0086"/>
    </dbReference>
    <dbReference type="GeneID" id="1450925"/>
    <dbReference type="KEGG" id="mja:MJ_0086"/>
    <dbReference type="eggNOG" id="arCOG03411">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005533_4_3_2"/>
    <dbReference type="InParanoid" id="Q57551"/>
    <dbReference type="OMA" id="CKCWELQ"/>
    <dbReference type="OrthoDB" id="44342at2157"/>
    <dbReference type="PhylomeDB" id="Q57551"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside-triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031725">
        <property type="entry name" value="ASMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003697">
        <property type="entry name" value="Maf-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="O_MeTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43213">
        <property type="entry name" value="PTHR43213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16864">
        <property type="entry name" value="Dimerisation2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106687" description="Uncharacterized protein MJ0086">
        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="227"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01020"/>
        </source>
    </evidence>
    <sequence length="372" mass="43285" checksum="E2665D92DE83E317" modified="1997-11-01" version="1">MLLKSPDKSPEKILKLFDEVYSKARIFYLLRTAIDLNLFEYLSSFKTAKELAEILDADLILMEYMLKILNELDLIESKVVSERIYYKNAEITNIYLKKDSNYSIINPIYSYFENIKNWENLADILKNKSNCSNMDVDNFFPKVVRRMADECKCWELQKVLNYMAKYEEFKNAKKLLDLAGGHGLYAIGFSMLNRNLKCYVFDLPNVIEETKKFIKKYNAKNVFTITGDFYKDDIGKGYDIIFCSYNPGGKNPKIAEKVYNALNEGGLFINKQFFPDKEEGIEDYINNMEWNFSKPEGLKKGKIRYTFEGDLNLNDYLKYLEDLGFKILEVVDMSELLGLDENSSSFRKSANPSDSLRFKDNSPAKMIVAKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q57554</accession>
    <name>Y089_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ0089</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0089</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64311">
        <property type="entry name" value="A64311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57554"/>
    <dbReference type="SMR" id="Q57554"/>
    <dbReference type="STRING" id="243232.MJ_0089"/>
    <dbReference type="EnsemblBacteria" id="AAB98070">
        <property type="protein sequence ID" value="AAB98070"/>
        <property type="gene ID" value="MJ_0089"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0089"/>
    <dbReference type="eggNOG" id="arCOG00198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="InParanoid" id="Q57554"/>
    <dbReference type="OMA" id="LPMYQRA"/>
    <dbReference type="PhylomeDB" id="Q57554"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093217" description="Uncharacterized ABC transporter ATP-binding protein MJ0089">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="6"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="254" mass="28528" checksum="379CB9A38BA787FA" modified="1997-11-01" version="1">MSLMILSVDGVEFAYKSRQILNNIKFEVKRGEVVSILGVNGAGKSTLLKCINKILKPKRGTILIDNFDIKNLDNLELAKKVGYVPQRAEGNYMTVFDAVLLGRKPHIKWEVSDRDIEITHKVLKLLNLEDYALRYTNELSGGELQKVIIARALVQEPQILLLDEPTNNLDLKNQLEVMKIIMDISKSQNIASIVVMHDLNLALRYSDKFIMLKDGVIYAEGGREVINPENIKAVYGVDAYIENVRGIPVVVPIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57588</accession>
    <name>T1R1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative type I restriction enzyme MjaVIIP endonuclease subunit</fullName>
            <shortName evidence="2">MjaVIIP</shortName>
            <shortName evidence="3">R protein</shortName>
            <ecNumber evidence="1">3.1.21.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0124</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">The restriction (R) subunit of a type I restriction enzyme that recognizes 5'-CAAN(7)TGG-3' and cleaves a random distance away. The R subunit is required for both endonuclease and ATPase activities but not for modification. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HsdR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57588"/>
    <dbReference type="STRING" id="243232.MJ_0124"/>
    <dbReference type="REBASE" id="154995">
        <property type="entry name" value="VscVS12ORF1031P"/>
    </dbReference>
    <dbReference type="REBASE" id="191862">
        <property type="entry name" value="Apa1447ORF2453P"/>
    </dbReference>
    <dbReference type="REBASE" id="191879">
        <property type="entry name" value="Apa1468ORF2954P"/>
    </dbReference>
    <dbReference type="REBASE" id="191891">
        <property type="entry name" value="Apa1468ORF2715P"/>
    </dbReference>
    <dbReference type="REBASE" id="203827">
        <property type="entry name" value="Bli141ORF4598P"/>
    </dbReference>
    <dbReference type="REBASE" id="203830">
        <property type="entry name" value="Bli27ORF807P"/>
    </dbReference>
    <dbReference type="REBASE" id="3900">
        <property type="entry name" value="MjaVIIP"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q57588"/>
    <dbReference type="EnsemblBacteria" id="AAB98104">
        <property type="protein sequence ID" value="AAB98104"/>
        <property type="gene ID" value="MJ_0124"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0124"/>
    <dbReference type="eggNOG" id="arCOG00878">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005762_0_0_2"/>
    <dbReference type="InParanoid" id="Q57588"/>
    <dbReference type="OMA" id="FFIANYF"/>
    <dbReference type="PhylomeDB" id="Q57588"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007409">
        <property type="entry name" value="Restrct_endonuc_type1_HsdR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004473">
        <property type="entry name" value="Restrct_endonuc_typeI_HsdR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040980">
        <property type="entry name" value="SWI2_SNF2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04313">
        <property type="entry name" value="HSDR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18766">
        <property type="entry name" value="SWI2_SNF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00348">
        <property type="entry name" value="hsdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000106704" description="Putative type I restriction enzyme MjaVIIP endonuclease subunit">
        <location>
            <begin position="1"/>
            <end position="1075"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P08956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1075" mass="127797" checksum="4F765E19E0B52889" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57626</accession>
    <name>Y162_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0162</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0162</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64320">
        <property type="entry name" value="C64320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869657.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57626"/>
    <dbReference type="SMR" id="Q57626"/>
    <dbReference type="STRING" id="243232.MJ_0162"/>
    <dbReference type="EnsemblBacteria" id="AAB98146">
        <property type="protein sequence ID" value="AAB98146"/>
        <property type="gene ID" value="MJ_0162"/>
    </dbReference>
    <dbReference type="GeneID" id="1451009"/>
    <dbReference type="KEGG" id="mja:MJ_0162"/>
    <dbReference type="eggNOG" id="arCOG00544">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="InParanoid" id="Q57626"/>
    <dbReference type="OMA" id="FSAHGDY"/>
    <dbReference type="OrthoDB" id="39954at2157"/>
    <dbReference type="PhylomeDB" id="Q57626"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106724" description="Uncharacterized protein MJ0162">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="421" mass="47477" checksum="5F5643116A67BABB" modified="1997-11-01" version="1">MVLLKFHGGCQQIGMSCVEVETQKGRVLLDCGMSPDTGEIPKVDDKAVDAVIVSHAHLDHCGAIPFYKFKKIYCTHPTADLMFITWRDTLNLTKAYKEEDIQHAMENIECLNYYEERQITENIKFKFYNAGHILGSASIYLEVDGKKILYTGDINEGVSRTLLPADTDIDEIDVLIIESTYGSPLDIKPARKTLERQLIEEISETIENGGKVIIPVFAIGRAQEILLIINNYIRSGKLRDVPIYTDGSLIHATAVYMSYINWLNPKIKNMVENRINPFGEIKKADESLVFNKEPCIIVSTSGMVQGGPVLKYLKLLKDPKNKLILTGYQAEGTLGRELEEGAKEIQPFKNKIPIRGKVVKIEFSAHGDYNSLVRYIKKIPKPEKAIVMHGERYQSLSFAMTIWKTLKIPTFVPVRGTILPI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57629</accession>
    <name>Y165_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0165</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0165</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64320">
        <property type="entry name" value="F64320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869660.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57629"/>
    <dbReference type="SMR" id="Q57629"/>
    <dbReference type="STRING" id="243232.MJ_0165"/>
    <dbReference type="EnsemblBacteria" id="AAB98147">
        <property type="protein sequence ID" value="AAB98147"/>
        <property type="gene ID" value="MJ_0165"/>
    </dbReference>
    <dbReference type="GeneID" id="1451012"/>
    <dbReference type="KEGG" id="mja:MJ_0165"/>
    <dbReference type="eggNOG" id="arCOG02465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065705_0_0_2"/>
    <dbReference type="InParanoid" id="Q57629"/>
    <dbReference type="OMA" id="EVIWGPG"/>
    <dbReference type="OrthoDB" id="73714at2157"/>
    <dbReference type="PhylomeDB" id="Q57629"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039476">
        <property type="entry name" value="P2CMN_synthase_LarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064">
        <property type="entry name" value="PTHR43064"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106727" description="Uncharacterized protein MJ0165">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="256" mass="28107" checksum="AB9DE5847D62D928" modified="1996-11-01" version="1">MGKNLRDLLLAFKNGDISLDEIEKQIKLNYYEEIEERLKLDINRQFRTGVPEVVYGKGKDIDEIIKATLKLVEKNGIALATKIEDIEKLSDEIRKWNLKNYDIKINKKAKTLIIKNKNYEVKKIGKVGILTAGTSDIPVAEEAKDTLEIMGVEAITAYDVGIAGIHRLFPALKRMIEEDVCCIIVVAGMEGALPSVIASMVDIPVIGVPTSTSYGIKITPLLTMLHSCSPGIAVVNIDNGFGAGVFAGLIAKIMHK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57726</accession>
    <name>FKBPS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Short-type peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">Short-type PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">FKBP-type peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">FKBP-type PPIase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Rotamase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0278</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the cis-trans isomerization of peptidyl prolyl bonds and accelerates protein folding. Also exhibits chaperone-like activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains an N-terminal PPIase domain and an IF (Insert in the Flap) domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64334">
        <property type="entry name" value="G64334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57726"/>
    <dbReference type="SMR" id="Q57726"/>
    <dbReference type="STRING" id="243232.MJ_0278"/>
    <dbReference type="EnsemblBacteria" id="AAB98266">
        <property type="protein sequence ID" value="AAB98266"/>
        <property type="gene ID" value="MJ_0278"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0278"/>
    <dbReference type="eggNOG" id="arCOG00981">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098197_2_0_2"/>
    <dbReference type="InParanoid" id="Q57726"/>
    <dbReference type="OMA" id="HSHEGGC"/>
    <dbReference type="PhylomeDB" id="Q57726"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0697">Rotamase</keyword>
    <feature type="chain" id="PRO_0000075376" description="Short-type peptidyl-prolyl cis-trans isomerase">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="IF" evidence="2">
        <location>
            <begin position="86"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O52980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q58235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="157" mass="17680" checksum="36C895C541FA0D44" modified="1996-11-01" version="1">MINLIKKGDYVKVDYILEVDGKVIDTSIEEVAKENKIYYPEREYEPIGFIVGNGELIEGFEEAVIGMEVGEEKTVTIPPEKGYGLRDERLIQEIPKEMFADADFEPQEGMLILASGIPAKIIKVTDDTVTLDFNHELAGKELKFTIKVRDVQPAESE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q57803</accession>
    <name>Y357_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0357</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0357</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64344">
        <property type="entry name" value="E64344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869856.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57803"/>
    <dbReference type="SMR" id="Q57803"/>
    <dbReference type="STRING" id="243232.MJ_0357"/>
    <dbReference type="EnsemblBacteria" id="AAB98349">
        <property type="protein sequence ID" value="AAB98349"/>
        <property type="gene ID" value="MJ_0357"/>
    </dbReference>
    <dbReference type="GeneID" id="1451214"/>
    <dbReference type="KEGG" id="mja:MJ_0357"/>
    <dbReference type="HOGENOM" id="CLU_136678_1_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051262">
        <property type="term" value="P:protein tetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003708">
        <property type="entry name" value="SecB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035958">
        <property type="entry name" value="SecB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36918">
        <property type="entry name" value="PTHR36918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02556">
        <property type="entry name" value="SecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54611">
        <property type="entry name" value="SSF54611"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106828" description="Uncharacterized protein MJ0357">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <sequence length="155" mass="17715" checksum="FBE28D58597DEEF7" modified="1996-11-01" version="1">MEAPPKCALKFSNYIVKHIEFILNEVPEKDEKIRLNVNIDTEIRYNKNEPNKFITIIKITAGEKKDFAKSPVYLSVEVWGFFEVIEEAIDKVRQFAEINSVAILFPYVRALISTITANANIPPVILPPINVAGMMANIEEVKEENTEKQETEAYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q57807</accession>
    <name>Y361_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0361</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0361</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64345">
        <property type="entry name" value="A64345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869860.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57807"/>
    <dbReference type="SMR" id="Q57807"/>
    <dbReference type="STRING" id="243232.MJ_0361"/>
    <dbReference type="EnsemblBacteria" id="AAB98353">
        <property type="protein sequence ID" value="AAB98353"/>
        <property type="gene ID" value="MJ_0361"/>
    </dbReference>
    <dbReference type="GeneID" id="1451218"/>
    <dbReference type="KEGG" id="mja:MJ_0361"/>
    <dbReference type="eggNOG" id="arCOG01057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_153628_1_0_2"/>
    <dbReference type="InParanoid" id="Q57807"/>
    <dbReference type="OMA" id="YQKEDKM"/>
    <dbReference type="OrthoDB" id="130706at2157"/>
    <dbReference type="PhylomeDB" id="Q57807"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000160632" description="Uncharacterized HTH-type transcriptional regulator MJ0361">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <sequence length="107" mass="12343" checksum="A1BF198CE20561EE" modified="1996-11-01" version="1">MILKILAKKHVKDVLKLLNSKDMYFSELQKTLNLHPKILDSILSDLVNEGFVEKREGESPYKFGKVYYSITPRGKRALEILDLIETFDTLREGQDIVINYKIVNSTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q57836</accession>
    <name>CBIT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)</fullName>
            <ecNumber evidence="1">2.1.1.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiT</name>
        <name type="ordered locus">MJ0391</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2013" name="BMC Struct. Biol." volume="13" first="10" last="10">
            <title>Crystal structure of putative CbiT from Methanocaldococcus jannaschii: an intermediate enzyme activity in cobalamin (vitamin B12) biosynthesis.</title>
            <authorList>
                <person name="Padmanabhan B."/>
                <person name="Yokoyama S."/>
                <person name="Bessho Y."/>
            </authorList>
            <dbReference type="PubMed" id="23688113"/>
            <dbReference type="DOI" id="10.1186/1472-6807-13-10"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 + CO2 + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36067"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="2.1.1.196"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64348">
        <property type="entry name" value="G64348"/>
    </dbReference>
    <dbReference type="PDB" id="2YXD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B=1-183"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YXD"/>
    <dbReference type="AlphaFoldDB" id="Q57836"/>
    <dbReference type="SMR" id="Q57836"/>
    <dbReference type="STRING" id="243232.MJ_0391"/>
    <dbReference type="EnsemblBacteria" id="AAB98376">
        <property type="protein sequence ID" value="AAB98376"/>
        <property type="gene ID" value="MJ_0391"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0391"/>
    <dbReference type="eggNOG" id="arCOG00977">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094143_0_0_2"/>
    <dbReference type="InParanoid" id="Q57836"/>
    <dbReference type="OMA" id="RCKFVYA"/>
    <dbReference type="PhylomeDB" id="Q57836"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57836"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043776">
        <property type="term" value="F:cobalt-precorrin-6B C5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046140">
        <property type="term" value="P:corrin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00786">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023475">
        <property type="entry name" value="CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134938" description="Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="38"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="48"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="67"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="84"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="92"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="102"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="126"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="167"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="176"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00786"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2YXD"/>
        </source>
    </evidence>
    <sequence length="183" mass="20502" checksum="CB47419BB8781340" modified="1996-11-01" version="1">MKYMIPDEEFIRREGVPITKEEIRAVSIGKLNLNKDDVVVDVGCGSGGMTVEIAKRCKFVYAIDYLDGAIEVTKQNLAKFNIKNCQIIKGRAEDVLDKLEFNKAFIGGTKNIEKIIEILDKKKINHIVANTIVLENAAKIINEFESRGYNVDAVNVFISYAKKIPSGHMFLAKNPITIIKAVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57839</accession>
    <name>SPT4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">spt4</name>
        <name type="synonym">rpoE''</name>
        <name evidence="3" type="synonym">rpoE2</name>
        <name type="ordered locus">MJ0396</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="Nucleic Acids Res." volume="38" first="4040" last="4051">
            <title>Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif.</title>
            <authorList>
                <person name="Hirtreiter A."/>
                <person name="Damsma G.E."/>
                <person name="Cheung A.C."/>
                <person name="Klose D."/>
                <person name="Grohmann D."/>
                <person name="Vojnic E."/>
                <person name="Martin A.C."/>
                <person name="Cramer P."/>
                <person name="Werner F."/>
            </authorList>
            <dbReference type="PubMed" id="20197319"/>
            <dbReference type="DOI" id="10.1093/nar/gkq135"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH ZINC</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15739363">
            <id>Q57839</id>
        </interactant>
        <interactant intactId="EBI-15739382">
            <id>Q57818</id>
            <label>spt5</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64349">
        <property type="entry name" value="D64349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869895.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3LPE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="B/D/F/H=1-59"/>
    </dbReference>
    <dbReference type="PDB" id="4ZN1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="B=2-59"/>
    </dbReference>
    <dbReference type="PDB" id="4ZN3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="B=2-59"/>
    </dbReference>
    <dbReference type="PDBsum" id="3LPE"/>
    <dbReference type="PDBsum" id="4ZN1"/>
    <dbReference type="PDBsum" id="4ZN3"/>
    <dbReference type="AlphaFoldDB" id="Q57839"/>
    <dbReference type="SMR" id="Q57839"/>
    <dbReference type="DIP" id="DIP-46332N"/>
    <dbReference type="IntAct" id="Q57839">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0396"/>
    <dbReference type="EnsemblBacteria" id="AAB98386">
        <property type="protein sequence ID" value="AAB98386"/>
        <property type="gene ID" value="MJ_0396"/>
    </dbReference>
    <dbReference type="GeneID" id="1451253"/>
    <dbReference type="KEGG" id="mja:MJ_0396"/>
    <dbReference type="eggNOG" id="arCOG04077">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199467_0_0_2"/>
    <dbReference type="InParanoid" id="Q57839"/>
    <dbReference type="OMA" id="VCRECHR"/>
    <dbReference type="OrthoDB" id="119723at2157"/>
    <dbReference type="PhylomeDB" id="Q57839"/>
    <dbReference type="EvolutionaryTrace" id="Q57839"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="PTHR40704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="SSF63393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000074037" description="Transcription elongation factor Spt4">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="2"/>
            <end position="4"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="5"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="9"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="17"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="22"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="30"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="20197319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="8688087"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3LPE"/>
        </source>
    </evidence>
    <sequence length="59" mass="6578" checksum="CFC635FF7C1B0919" modified="1997-11-01" version="1">MRACLKCKYLTNDEICPICHSPTSENWIGLLIVINPEKSEIAKKAGIDIKGKYALSVKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q57876</accession>
    <name>Y434_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Gamma-glutamylcyclotransferase and putative RNase MJ0434</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName>Gamma-glutamylcyclotransferase family protein MJ0434</fullName>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>Putative RNase MJ0434</fullName>
                <ecNumber evidence="2">3.1.-.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Putative toxin MJ0434</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name type="ordered locus">MJ0434</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable toxic component of a putative type VII toxin-antitoxin (TA) system, probably an RNase. Probably neutralized by cognate antitoxin MJ0435. Neutralization may be due to AMPylation by MJ0435.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer, probably forms a complex with cognate antitoxin MJ0435.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Modified by cognate antitoxin MJ0435; probably at least 2 successive AMPylation events occur on Tyr-82.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HepT RNase toxin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the gamma-glutamylcyclotransferase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64354">
        <property type="entry name" value="B64354"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57876"/>
    <dbReference type="SMR" id="Q57876"/>
    <dbReference type="STRING" id="243232.MJ_0434"/>
    <dbReference type="EnsemblBacteria" id="AAB98422">
        <property type="protein sequence ID" value="AAB98422"/>
        <property type="gene ID" value="MJ_0434"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0434"/>
    <dbReference type="eggNOG" id="arCOG05024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG05099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243026_0_0_2"/>
    <dbReference type="InParanoid" id="Q57876"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="SSF110857"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000158262" description="Gamma-glutamylcyclotransferase and putative RNase MJ0434">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RX(4)HXY motif" evidence="1">
        <location>
            <begin position="75"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-di-AMP-tyrosine" evidence="1">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJR1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="222" mass="26742" checksum="F0FA33F1634D1A11" modified="1997-11-01" version="1">MRKDVKIYLNHILESIELIEEYTKDKTEDDFFTSKFLQDAVIRRIEIIGEAIKNLPMEFREKYNHIPWKEFAEMRDILIRKYFGVDLGLTWEVVKKDIPKLKEEILKIMEELDKNKNNKYNVFAYGELMKKERLLELINRVPKMIEGRVYGYEKFFDETIGYYGARKKEGSYIDGIILLDITDKELGIFDDYEDLDVYYIREKTTAVSEDGRKYDVYIYLRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q57895</accession>
    <name>Y453_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0453</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0453</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64356">
        <property type="entry name" value="E64356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869952.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57895"/>
    <dbReference type="SMR" id="Q57895"/>
    <dbReference type="STRING" id="243232.MJ_0453"/>
    <dbReference type="EnsemblBacteria" id="AAB98442">
        <property type="protein sequence ID" value="AAB98442"/>
        <property type="gene ID" value="MJ_0453"/>
    </dbReference>
    <dbReference type="GeneID" id="1451314"/>
    <dbReference type="KEGG" id="mja:MJ_0453"/>
    <dbReference type="eggNOG" id="arCOG04844">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159086_1_0_2"/>
    <dbReference type="InParanoid" id="Q57895"/>
    <dbReference type="OMA" id="TKPCYGT"/>
    <dbReference type="OrthoDB" id="100968at2157"/>
    <dbReference type="PhylomeDB" id="Q57895"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012031">
        <property type="entry name" value="UCP006577"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08979">
        <property type="entry name" value="DUF1894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006577">
        <property type="entry name" value="UCP006577"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106883" description="Uncharacterized protein MJ0453">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="107" mass="12603" checksum="4E3723628AEF3F8F" modified="1996-11-01" version="1">MGCIDKLNYEILYKGGFKECAEYIRKNFKNIKEMEAGYEIFEGIFLIGIPPIPVAYEDNYVIFPYTKPCYGTFVLKINLDEINKDKKEEKKEKDKGKKGLLSRLKFW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57942</accession>
    <name>Y522_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0522</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0522</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64365">
        <property type="entry name" value="B64365"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870025.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57942"/>
    <dbReference type="SMR" id="Q57942"/>
    <dbReference type="STRING" id="243232.MJ_0522"/>
    <dbReference type="EnsemblBacteria" id="AAB98514">
        <property type="protein sequence ID" value="AAB98514"/>
        <property type="gene ID" value="MJ_0522"/>
    </dbReference>
    <dbReference type="GeneID" id="1451386"/>
    <dbReference type="KEGG" id="mja:MJ_0522"/>
    <dbReference type="eggNOG" id="arCOG04833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253609_0_0_2"/>
    <dbReference type="OMA" id="EMFRTPG"/>
    <dbReference type="OrthoDB" id="75706at2157"/>
    <dbReference type="PhylomeDB" id="Q57942"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017059">
        <property type="entry name" value="NiFe-hyd_3_EhaH_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10125">
        <property type="entry name" value="NADHdeh_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036536">
        <property type="entry name" value="EhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106911" description="Uncharacterized protein MJ0522">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="218" mass="23897" checksum="2E2EC3ACE9714CA5" modified="1996-11-01" version="1">MIESITGYLFGIVPFGDIVFGFSEFSIIGFITAVIFTIIVYLTKPEKQLEAQKFKIEDKLEVVTLNELKIRRMMAIVCGIATAGAMLTYDLFDYALFLTLVGIANIGIVSAVKREWVLNASYQYGLIAMIATLPLFGSAGMILAKTGTLSIFELPKIQTSLLFEKIIFAAGMAGETGIAPFYAAKAEMFRAPGSPYILMIHLSSLLLIVRTVEILLTI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q57954</accession>
    <name>Y534_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0534</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0534</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64366">
        <property type="entry name" value="F64366"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57954"/>
    <dbReference type="SMR" id="Q57954"/>
    <dbReference type="STRING" id="243232.MJ_0534"/>
    <dbReference type="PRIDE" id="Q57954"/>
    <dbReference type="EnsemblBacteria" id="AAB98525">
        <property type="protein sequence ID" value="AAB98525"/>
        <property type="gene ID" value="MJ_0534"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0534"/>
    <dbReference type="eggNOG" id="arCOG00509">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017490_0_0_2"/>
    <dbReference type="InParanoid" id="Q57954"/>
    <dbReference type="OMA" id="AFGLHYC"/>
    <dbReference type="PhylomeDB" id="Q57954"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106919" description="Uncharacterized protein MJ0534">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="247"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00088"/>
        </source>
    </evidence>
    <sequence length="391" mass="45532" checksum="C08636FC7C18D516" modified="1998-12-15" version="2">MVKYMVLKIKDNIYCMSFIEWKIKEYRGLDIEKGTTYNSYLILDKNNVIIDTTRIKYFDELLSYLKDVANLKLDYIISNHISPDHNECIEKLIELTEAKIVTTKIRKYYLDAQFNTKDWEFVIVKNGDELNIGNRTLKFITDDKCEYMLTYCVEDKILFSNDLFSQHVVYKEKIDSDIGHKIMLDAKEYFANILLPYRKSILKILNILKDLDLEYICPSHGVIWHIMIDEILTKYRMWCSDSYKNTAVIVYATIYSSTEKIAKALGEGLSEEGVDVIYHRLDTSPLNIIMRDILDAKYVLVGSPTINMNVHPKVGMLLTYIEGLKPSNKKIGVAFGSYGWKECATKKIIEAFKRLGFKIVDDKILTFRFAPKENDIKKIKEFGRKLAKINV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57955</accession>
    <name>Y535_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0535</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0535</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative deacetylase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone deacetylase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64366">
        <property type="entry name" value="G64366"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57955"/>
    <dbReference type="SMR" id="Q57955"/>
    <dbReference type="STRING" id="243232.MJ_0535"/>
    <dbReference type="EnsemblBacteria" id="AAB98526">
        <property type="protein sequence ID" value="AAB98526"/>
        <property type="gene ID" value="MJ_0535"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0535"/>
    <dbReference type="eggNOG" id="arCOG00324">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007727_8_2_2"/>
    <dbReference type="InParanoid" id="Q57955"/>
    <dbReference type="OMA" id="GRAMGFC"/>
    <dbReference type="PhylomeDB" id="Q57955"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016575">
        <property type="term" value="P:histone deacetylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="His_deacetylse"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="SSF52768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000114745" description="Uncharacterized protein MJ0535">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="343" mass="38174" checksum="8848EDB757FDC233" modified="1996-11-01" version="1">MVSIMPKILYNPEVLGHKPKSYHVENPERVLTILNSLKSNGFDDIVLIEGKSTINEILEIHSRDYVYSIINLSKSFNYYDGDTYLCDRTLDAALTAFKLAKEAVKLALKDRDLYFALTRPPGHHAGISGRALGAMSNGFCIFNNIAGAARLAKNYMKKVIIIDFDVHHGNGTQEIFWNDNRVIHIDFHQRGIYPGTGDILDIGGEEAKGTKINLPFPAHSTDADYIFAWNEIVEPILNYFSPDTVLVSAGFDAFINDGLASMDLTETFYRFVGAKLSGYSVTAVLEGGYSIGLKYAPPAFLDGYVDAKDVLDNLEDYTVINSNEVKSMVKNVKKIIGEYLDIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58048</accession>
    <name>Y631_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative hydrogenase maturation protease MJ0631</fullName>
            <ecNumber>3.4.23.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0631</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64378">
        <property type="entry name" value="G64378"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870136.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58048"/>
    <dbReference type="SMR" id="Q58048"/>
    <dbReference type="STRING" id="243232.MJ_0631"/>
    <dbReference type="MEROPS" id="A31.003"/>
    <dbReference type="EnsemblBacteria" id="AAB98623">
        <property type="protein sequence ID" value="AAB98623"/>
        <property type="gene ID" value="MJ_0631"/>
    </dbReference>
    <dbReference type="GeneID" id="1451497"/>
    <dbReference type="KEGG" id="mja:MJ_0631"/>
    <dbReference type="eggNOG" id="arCOG04429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099037_4_1_2"/>
    <dbReference type="InParanoid" id="Q58048"/>
    <dbReference type="OMA" id="EMCAANP"/>
    <dbReference type="OrthoDB" id="124075at2157"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036211">
        <property type="term" value="P:protein modification process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06067">
        <property type="entry name" value="H2MP_MemB-H2evol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004420">
        <property type="entry name" value="Pept_A31_hyd_mat_HycI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00142">
        <property type="entry name" value="hycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0064">Aspartyl protease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000201949" description="Putative hydrogenase maturation protease MJ0631">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="169" mass="19271" checksum="398028DD88236EE7" modified="1996-11-01" version="1">MKEMLLDKLKNCKKLVIMGIGNELKGDDAVGIYVVKKLMRYFGEEKEFINIKNLYLINAGTVPDFFTDILKEIKPTHILIIDCALMDKDVGEVKIIKEDEIINYSFSTHTLPLSIIVKYLKKFINAEIIILGIQPKIIDFCPISEEVKLSGDKLVNTLIEIIEELKLAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58069</accession>
    <name>Y653_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0653</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0653</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64381">
        <property type="entry name" value="E64381"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870158.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58069"/>
    <dbReference type="SMR" id="Q58069"/>
    <dbReference type="STRING" id="243232.MJ_0653"/>
    <dbReference type="EnsemblBacteria" id="AAB98648">
        <property type="protein sequence ID" value="AAB98648"/>
        <property type="gene ID" value="MJ_0653"/>
    </dbReference>
    <dbReference type="GeneID" id="1451519"/>
    <dbReference type="KEGG" id="mja:MJ_0653"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_7_0_2"/>
    <dbReference type="InParanoid" id="Q58069"/>
    <dbReference type="OMA" id="APIKVYM"/>
    <dbReference type="OrthoDB" id="83765at2157"/>
    <dbReference type="PhylomeDB" id="Q58069"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044065">
        <property type="entry name" value="ACP_MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51901">
        <property type="entry name" value="ACP_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000106972" description="Uncharacterized protein MJ0653">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="ACP-type MB" evidence="2">
        <location>
            <begin position="159"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01249"/>
        </source>
    </evidence>
    <sequence length="194" mass="21723" checksum="33921320EF82E5DC" modified="1996-11-01" version="1">MKIACDIPVSEVMSFPVIKATKNMSIYDIANIMTENNIGAVVIVENNKPIGIVTERDIVKRVVSKNLKPKDVLAEEVMSKKIITIPQNASITEAAKIMATHGIKRLPVVKDGELVGIVTQSDIVRVSPELLEIVIEYASITPEEKEVISLDTDEFSEEYINGICENCGYQGRVRLYQGRYLCDECIEEFEEKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58087</accession>
    <name>Y674_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0674</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0674</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64384">
        <property type="entry name" value="B64384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870179.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58087"/>
    <dbReference type="SMR" id="Q58087"/>
    <dbReference type="STRING" id="243232.MJ_0674"/>
    <dbReference type="PRIDE" id="Q58087"/>
    <dbReference type="EnsemblBacteria" id="AAB98668">
        <property type="protein sequence ID" value="AAB98668"/>
        <property type="gene ID" value="MJ_0674"/>
    </dbReference>
    <dbReference type="GeneID" id="1451540"/>
    <dbReference type="KEGG" id="mja:MJ_0674"/>
    <dbReference type="eggNOG" id="arCOG00934">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062674_0_1_2"/>
    <dbReference type="InParanoid" id="Q58087"/>
    <dbReference type="OMA" id="GGEPTPN"/>
    <dbReference type="OrthoDB" id="34153at2157"/>
    <dbReference type="PhylomeDB" id="Q58087"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040085">
        <property type="entry name" value="MJ0674-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43075">
        <property type="entry name" value="PTHR43075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01099">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000106984" description="Uncharacterized protein MJ0674">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="111"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="338" mass="40020" checksum="96923543AEB9C705" modified="1996-11-01" version="1">MKLGRYLAVSKDLAPAKFIIAKCIEVEEFKGLELNELWEIHNKVLKKVDFDNFDFNDLEYVKPNLLDLKVEIAKNIFKNCHFCEHRCYVNRETERGFCRIKESYYSTEFLHLGEERVLVPSHTIFFCGCNFKCVFCQNWDISQVYFDKTIPNHCIPYNPKEMAKIIKHKRDYSKNVNFVGGDPTPHLLSILKTLSYLDKNIPVVWNSNMYLTVEGMHLLKGVVDVYLTDFKFGNNECGERLSKVKNYFDIIKRNHLLIKDEEVIIRHLVMPNHLDCCTEKIFDFISKNLDNAVVNVMFQYRPEYKAKEYPDINRRLTYEEIEKALELAEKYNLDLIYD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58093</accession>
    <name>RPE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="1">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpe</name>
        <name type="ordered locus">MJ0680</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64384">
        <property type="entry name" value="H64384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870185.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58093"/>
    <dbReference type="SMR" id="Q58093"/>
    <dbReference type="STRING" id="243232.MJ_0680"/>
    <dbReference type="EnsemblBacteria" id="AAB98675">
        <property type="protein sequence ID" value="AAB98675"/>
        <property type="gene ID" value="MJ_0680"/>
    </dbReference>
    <dbReference type="GeneID" id="1451546"/>
    <dbReference type="KEGG" id="mja:MJ_0680"/>
    <dbReference type="eggNOG" id="arCOG05046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054856_2_1_2"/>
    <dbReference type="InParanoid" id="Q58093"/>
    <dbReference type="OMA" id="WLQVDGG"/>
    <dbReference type="OrthoDB" id="78990at2157"/>
    <dbReference type="PhylomeDB" id="Q58093"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044262">
        <property type="term" value="P:cellular carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="PTHR11749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000171584" description="Ribulose-phosphate 3-epimerase">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="194"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <sequence length="234" mass="25838" checksum="070E2CF7A2BC9D7F" modified="1996-11-01" version="1">MIKIGASILSADFGHLREEIKKAEEAGVDFFHVDMMDGHFVPNISMGIGIAKHVKKLTELPVEVHLMVENVDLFVNEFEEMDYITFHIEAVKFPFRIINRIKSIGAKPIVALNPATPLDAIEYILGDVYAVLVMTVEPGFSGQKFIPVMTKKIRKLKSMIVENGYDTKIFVDGGINVETAPLAVKAGADVLVAASAIFGKDDVKTAVKNLREAALEALNKDFLTKSFNSNEEKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58099</accession>
    <name>Y686_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0686</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0686</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64385">
        <property type="entry name" value="F64385"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870191.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58099"/>
    <dbReference type="SMR" id="Q58099"/>
    <dbReference type="STRING" id="243232.MJ_0686"/>
    <dbReference type="EnsemblBacteria" id="AAB98681">
        <property type="protein sequence ID" value="AAB98681"/>
        <property type="gene ID" value="MJ_0686"/>
    </dbReference>
    <dbReference type="GeneID" id="1451552"/>
    <dbReference type="KEGG" id="mja:MJ_0686"/>
    <dbReference type="eggNOG" id="arCOG05223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021082_0_0_2"/>
    <dbReference type="InParanoid" id="Q58099"/>
    <dbReference type="OMA" id="KECEAGM"/>
    <dbReference type="OrthoDB" id="106124at2157"/>
    <dbReference type="PhylomeDB" id="Q58099"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004919">
        <property type="entry name" value="DUF262"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03235">
        <property type="entry name" value="DUF262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106989" description="Uncharacterized protein MJ0686">
        <location>
            <begin position="1"/>
            <end position="580"/>
        </location>
    </feature>
    <sequence length="580" mass="68393" checksum="C23CA146575B2506" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58107</accession>
    <name>Y696_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0696</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0696</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0803.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64386">
        <property type="entry name" value="H64386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870201.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58107"/>
    <dbReference type="SMR" id="Q58107"/>
    <dbReference type="STRING" id="243232.MJ_0696"/>
    <dbReference type="EnsemblBacteria" id="AAB98697">
        <property type="protein sequence ID" value="AAB98697"/>
        <property type="gene ID" value="MJ_0696"/>
    </dbReference>
    <dbReference type="GeneID" id="1451563"/>
    <dbReference type="KEGG" id="mja:MJ_0696"/>
    <dbReference type="eggNOG" id="arCOG09672">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1478917_0_0_2"/>
    <dbReference type="OMA" id="ILTICTI"/>
    <dbReference type="OrthoDB" id="119846at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009328">
        <property type="entry name" value="DUF986"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06173">
        <property type="entry name" value="DUF986"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106993" description="Uncharacterized protein MJ0696">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="182" mass="21487" checksum="62D4DD70A40952AB" modified="1996-11-01" version="1">MESILFIAIAFLINSFISYKITNMQPKIKSRIFKRVKMHYLNLIEGKKAEFDKKAMPILFGFMIIALISFNILLYVVYNCPVSITSIIAEILIIISMIIIWKAFNKEISVYLCDDGIYYSNKFISWKNIENVKKDDGFIVLFGKKKKILGRKLYLLQRIYLKYDEEIENIIKNQIEKFRDKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58112</accession>
    <name>PRIL_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priL</name>
        <name type="ordered locus">MJ0701</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64387">
        <property type="entry name" value="E64387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870206.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58112"/>
    <dbReference type="STRING" id="243232.MJ_0701"/>
    <dbReference type="EnsemblBacteria" id="AAB98698">
        <property type="protein sequence ID" value="AAB98698"/>
        <property type="gene ID" value="MJ_0701"/>
    </dbReference>
    <dbReference type="GeneID" id="1451568"/>
    <dbReference type="KEGG" id="mja:MJ_0701"/>
    <dbReference type="eggNOG" id="arCOG03013">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_759942_0_0_2"/>
    <dbReference type="InParanoid" id="Q58112"/>
    <dbReference type="OMA" id="HYARRSF"/>
    <dbReference type="OrthoDB" id="29893at2157"/>
    <dbReference type="PhylomeDB" id="Q58112"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007238">
        <property type="entry name" value="DNA_primase_lsu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106995" description="DNA primase large subunit PriL">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="370"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="376"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <sequence length="414" mass="49101" checksum="B53D6FEEB506CAF1" modified="1996-11-01" version="1">MIIMLSEYLEEFKEYLERFKNIDINFSDVLKMSKKFIIWRLKQIFGDSSTIFTNISSEITIFDKIFQMIDYDIDGEVEKRLPKDESRFMIGVRREKEIEIKKEIITNLLDFLLIILLSHTPYFNAFVRKYAEIKKIKVIKKLPNKISVWEFIKIASRSRINDLHLERLDLENGFVDITKIKEIFAKEIIRVELMKLGENIKKRKLPDDSVVKELLNEISDYLKDKVKYEQISGIKALNYKGNIPLEWHPPCIRGILNDILSGGSPSHYARRSFVVYWFCAKFNPNLRPLDKNGNLVNVSATDIASEEEIERFIDELIEMLFKNVEDFDEKKTRYYIMHNIGYKVGHGRLTHCEYCKNWQDDGGKGLSYYCKPDELCKKKFIIHPLDYLCYNINKHLKKERFKKIKKEDKNGDNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58114</accession>
    <name>Y703_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0703</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0703</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64387">
        <property type="entry name" value="G64387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870208.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58114"/>
    <dbReference type="SMR" id="Q58114"/>
    <dbReference type="STRING" id="243232.MJ_0703"/>
    <dbReference type="EnsemblBacteria" id="AAB98695">
        <property type="protein sequence ID" value="AAB98695"/>
        <property type="gene ID" value="MJ_0703"/>
    </dbReference>
    <dbReference type="GeneID" id="1451570"/>
    <dbReference type="KEGG" id="mja:MJ_0703"/>
    <dbReference type="eggNOG" id="arCOG00616">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_2_0_2"/>
    <dbReference type="InParanoid" id="Q58114"/>
    <dbReference type="OMA" id="LWVDAGF"/>
    <dbReference type="OrthoDB" id="84942at2157"/>
    <dbReference type="PhylomeDB" id="Q58114"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004650">
        <property type="entry name" value="HisA/F-archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="PTHR43090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00734">
        <property type="entry name" value="hisAF_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142292" description="Uncharacterized protein MJ0703">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="226" mass="25067" checksum="0B26652F68FF54EE" modified="1996-11-01" version="1">MKIIPVIDLKDKIAVHGKSGNRDEYKPLESVICKSSNPIEVAKAYKERGAKTIYIADLNFIMGNGDNFDIIKEIDFINKIVDIGVKSREDLETIKKVLNKDDRAIVATETLKDIELLKEKDIVVSLDFKNGNLLNYSLDEILSCVRDDTPLIILDISSVGTQRGVNAELIKYVLDKTNNPVYVGGGIKGMEDLELCYNLGVDAVLIATAIHKGVLDLEEIINKFGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q58123</accession>
    <name>HYPF_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable carbamoyltransferase HypF</fullName>
            <ecNumber evidence="1">6.2.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Carbamoyl phosphate-converting enzyme HypF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>[NiFe]-hydrogenase maturation factor HypF</fullName>
            <shortName>Hydrogenase maturation protein HypF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hypF</name>
        <name type="ordered locus">MJ0713</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the maturation of [NiFe] hydrogenases. Along with HypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases. HypF functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carboxamido moiety in an ATP-dependent reaction to the thiolate of the C-terminal cysteine of HypE yielding a protein-S-carboxamide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + diphosphate + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:55636"/>
            <dbReference type="Rhea" id="RHEA-COMP:14247"/>
            <dbReference type="Rhea" id="RHEA-COMP:14392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:76913"/>
            <dbReference type="ChEBI" id="CHEBI:139126"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; [NiFe] hydrogenase maturation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the carbamoyltransferase HypF family.</text>
    </comment>
    <dbReference type="EC" id="6.2.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64389">
        <property type="entry name" value="A64389"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58123"/>
    <dbReference type="SMR" id="Q58123"/>
    <dbReference type="STRING" id="243232.MJ_0713"/>
    <dbReference type="EnsemblBacteria" id="AAB98708">
        <property type="protein sequence ID" value="AAB98708"/>
        <property type="gene ID" value="MJ_0713"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0713"/>
    <dbReference type="eggNOG" id="arCOG01187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009164_0_0_2"/>
    <dbReference type="InParanoid" id="Q58123"/>
    <dbReference type="OMA" id="VQHHYAH"/>
    <dbReference type="PhylomeDB" id="Q58123"/>
    <dbReference type="UniPathway" id="UPA00335"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046944">
        <property type="term" value="P:protein carbamoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004421">
        <property type="entry name" value="Carbamoyltransferase_HypF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041440">
        <property type="entry name" value="HypF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011125">
        <property type="entry name" value="Znf_HypF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17788">
        <property type="entry name" value="HypF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07503">
        <property type="entry name" value="zf-HYPF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006256">
        <property type="entry name" value="CMPcnvr_hdrg_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00143">
        <property type="entry name" value="hypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000071619" description="Probable carbamoyltransferase HypF">
        <location>
            <begin position="1"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="3">
        <location>
            <begin position="11"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="YrdC-like" evidence="2">
        <location>
            <begin position="209"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="167"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P30131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="766" mass="87605" checksum="1B81EAB8D16CBFAD" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58134</accession>
    <name>OGG1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">8-oxoguanine DNA glycosylase/AP lyase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1 5">8-oxoguanine DNA glycosylase</fullName>
                <shortName evidence="1">8-oxoG DNA glycosylase</shortName>
                <ecNumber evidence="1 2 3">3.2.2.-</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1 6">DNA-(apurinic or apyrimidinic site) lyase</fullName>
                <shortName evidence="1 6">AP lyase</shortName>
                <ecNumber evidence="1 2 3">4.2.99.18</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">ogg</name>
        <name type="ordered locus">MJ0724</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Biol. Chem." volume="274" first="30447" last="30450">
            <title>Characterization of an 8-oxoguanine DNA glycosylase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Gogos A."/>
                <person name="Clarke N.D."/>
            </authorList>
            <dbReference type="PubMed" id="10521423"/>
            <dbReference type="DOI" id="10.1074/jbc.274.43.30447"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF LYS-129</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="journal article" date="2009" name="Structure" volume="17" first="703" last="712">
            <title>Crystal structures of two archaeal 8-oxoguanine DNA glycosylases provide structural insight into guanine/8-oxoguanine distinction.</title>
            <authorList>
                <person name="Faucher F."/>
                <person name="Duclos S."/>
                <person name="Bandaru V."/>
                <person name="Wallace S.S."/>
                <person name="Doublie S."/>
            </authorList>
            <dbReference type="PubMed" id="19446526"/>
            <dbReference type="DOI" id="10.1016/j.str.2009.03.007"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVE SITE</scope>
        <scope>MUTAGENESIS OF 205-VAL--LYS-207</scope>
    </reference>
    <reference evidence="9" key="4">
        <citation type="journal article" date="2010" name="J. Mol. Biol." volume="397" first="46" last="56">
            <title>The C-terminal lysine of Ogg2 DNA glycosylases is a major molecular determinant for guanine/8-oxoguanine distinction.</title>
            <authorList>
                <person name="Faucher F."/>
                <person name="Wallace S.S."/>
                <person name="Doublie S."/>
            </authorList>
            <dbReference type="PubMed" id="20083120"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2010.01.024"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF MUTANT GLN-129 IN COMPLEX WITH DNA CONTAINING 8-OXOGUANINE</scope>
        <scope>DOMAIN</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the excision of an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine = 8-oxoG) from DNA (PubMed:10521423, PubMed:19446526). Also cleaves the DNA backbone at apurinic/apyrimidinic sites (AP sites) (PubMed:10521423, PubMed:19446526). Has little specificity for the base opposite oxoG (PubMed:10521423).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 8.5.</text>
        </phDependence>
    </comment>
    <comment type="domain">
        <text evidence="4">Contains two domains separated by the central HhH motif. The C-terminal domain undergoes a conformational change upon DNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the type-2 OGG1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="1 2 3"/>
    <dbReference type="EC" id="4.2.99.18" evidence="1 2 3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64390">
        <property type="entry name" value="D64390"/>
    </dbReference>
    <dbReference type="PDB" id="3FHF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-207"/>
    </dbReference>
    <dbReference type="PDB" id="3KNT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B/C/D=1-207"/>
    </dbReference>
    <dbReference type="PDBsum" id="3FHF"/>
    <dbReference type="PDBsum" id="3KNT"/>
    <dbReference type="AlphaFoldDB" id="Q58134"/>
    <dbReference type="SMR" id="Q58134"/>
    <dbReference type="STRING" id="243232.MJ_0724"/>
    <dbReference type="PRIDE" id="Q58134"/>
    <dbReference type="EnsemblBacteria" id="AAB98720">
        <property type="protein sequence ID" value="AAB98720"/>
        <property type="gene ID" value="MJ_0724"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0724"/>
    <dbReference type="eggNOG" id="arCOG04357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104937_0_0_2"/>
    <dbReference type="InParanoid" id="Q58134"/>
    <dbReference type="OMA" id="FCILTAN"/>
    <dbReference type="PhylomeDB" id="Q58134"/>
    <dbReference type="BRENDA" id="3.2.2.B5">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="4.2.99.18">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58134"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016799">
        <property type="term" value="F:hydrolase activity, hydrolyzing N-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00241">
        <property type="entry name" value="Ogg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012092">
        <property type="entry name" value="DNA_glyclase/AP_lyase_Ogg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005954">
        <property type="entry name" value="Thrmst_ogg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159562" description="8-oxoguanine DNA glycosylase/AP lyase">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 7">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" evidence="1 7">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="site" description="Important for guanine/8-oxoguanine distinction" evidence="1 3 4">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="2">
        <original>K</original>
        <variation>S</variation>
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Unable to excise the damaged base. Slight decrease in lyase activity." evidence="3">
        <location>
            <begin position="205"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="34"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="52"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="65"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="102"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="148"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="159"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10521423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="19446526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="20083120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="19446526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="19446526"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="3FHF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="3KNT"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="3FHF"/>
        </source>
    </evidence>
    <sequence length="207" mass="24827" checksum="D17747917BF10B45" modified="1996-11-01" version="1">MMLIKKIEELKNSEIKDIIDKRIQEFKSFKNKSNEEWFKELCFCILTANFTAEGGIRIQKEIGDGFLTLPREELEEKLKNLGHRFYRKRAEYIVLARRFKNIKDIVESFENEKVAREFLVRNIKGIGYKEASHFLRNVGYDDVAIIDRHILRELYENNYIDEIPKTLSRRKYLEIENILRDIGEEVNLKLSELDLYIWYLRTGKVLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58143</accession>
    <name>Y733_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0733</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0733</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64391">
        <property type="entry name" value="E64391"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58143"/>
    <dbReference type="SMR" id="Q58143"/>
    <dbReference type="STRING" id="243232.MJ_0733"/>
    <dbReference type="EnsemblBacteria" id="AAB98743">
        <property type="protein sequence ID" value="AAB98743"/>
        <property type="gene ID" value="MJ_0733"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0733"/>
    <dbReference type="eggNOG" id="arCOG01670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167520_0_0_2"/>
    <dbReference type="OMA" id="ENDLFGH"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DrsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="SSF75169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107008" description="Uncharacterized protein MJ0733">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <sequence length="121" mass="14094" checksum="D1A69F7091B27BC7" modified="1996-11-01" version="1">MRWGFMKVAFLIFSYFHKNQPNMPVMMHTLLFANELKEKGDEVKIILEGEAVLWAKDLLSENHPLKSHFEKVKDDFVVCEACASMFNVKEEIKGKLKLENDLFGHVSLKKYLDGGYRIIEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58153</accession>
    <name>HDRB1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CoB--CoM heterodisulfide reductase subunit B 1</fullName>
            <ecNumber evidence="2">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrB1</name>
        <name type="ordered locus">MJ0743</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HdrB family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64392">
        <property type="entry name" value="G64392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870248.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58153"/>
    <dbReference type="SMR" id="Q58153"/>
    <dbReference type="STRING" id="243232.MJ_0743"/>
    <dbReference type="PRIDE" id="Q58153"/>
    <dbReference type="EnsemblBacteria" id="AAB98737">
        <property type="protein sequence ID" value="AAB98737"/>
        <property type="gene ID" value="MJ_0743"/>
    </dbReference>
    <dbReference type="GeneID" id="1451620"/>
    <dbReference type="KEGG" id="mja:MJ_0743"/>
    <dbReference type="eggNOG" id="arCOG00338">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052147_1_0_2"/>
    <dbReference type="InParanoid" id="Q58153"/>
    <dbReference type="OMA" id="SCCGASH"/>
    <dbReference type="OrthoDB" id="73750at2157"/>
    <dbReference type="PhylomeDB" id="Q58153"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017678">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03288">
        <property type="entry name" value="CoB_CoM_SS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150065" description="CoB--CoM heterodisulfide reductase subunit B 1">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LY38"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="294" mass="33310" checksum="C8D5DDCE6FDC8560" modified="1996-11-01" version="1">MEFVFFLGCIAPNRYPGIEKATYITMEKLGIKLHPFEKASCCPAPGVFGSFDLKTWLTLAARNLCMAEEVEMDILTICNGCYGSLYEANHLLKENEKARKMVNEILSKYGLEYKGKVRVRHLPEVLYYDLGVDRIKEEITNPLNVNVAVHYGCHYLKPTDIKKLESSERPRSFDELVEALGAVSVNYKDKNMCCGAGGGVRARNLDVALKMTKTKLENIKEAKADCITEVCPFCHLQFDRGQVEIKEKFGEEYNIPVIHYSQLLGLAMGMSPKDVALDLHFIPTDEFIKKIDRH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58165</accession>
    <name>Y755_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0755</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0755</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64394">
        <property type="entry name" value="C64394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870260.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58165"/>
    <dbReference type="SMR" id="Q58165"/>
    <dbReference type="STRING" id="243232.MJ_0755"/>
    <dbReference type="EnsemblBacteria" id="AAB98748">
        <property type="protein sequence ID" value="AAB98748"/>
        <property type="gene ID" value="MJ_0755"/>
    </dbReference>
    <dbReference type="GeneID" id="1451632"/>
    <dbReference type="KEGG" id="mja:MJ_0755"/>
    <dbReference type="eggNOG" id="arCOG00388">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054697_1_0_2"/>
    <dbReference type="OMA" id="WGNFSND"/>
    <dbReference type="OrthoDB" id="48337at2157"/>
    <dbReference type="PhylomeDB" id="Q58165"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013995" description="Uncharacterized protein MJ0755">
        <location>
            <begin position="19"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="342" mass="38778" checksum="BD25220A7EEAD85C" modified="1996-11-01" version="1" precursor="true">MWKKLMLLLLMAIPLVSAVAIPSISATDVVLVSDNCADQCTALEVADALNATVITTEWGIYNESLIDEILALNPDKVIIIGGPLAVVENYTTALENVGITVERIGGSNRYETNANVTLRFQNQFRYAFGNNTTVCVCHGFDDIALNETMGLIKNGTCLVLLTNGVNLSVEPQKLQLRINKVEIIENPICPFCNYSKLMLKLQKNGLKIEIKQIPKVKVKLMLQNRIRIMERRILMLKRMGVNVTDLEEKLKEVEQLMEQNRYQEAYRIMVQLQEEQMVRVKLHLHPMWSKMKRGKIQENKNASHIYHQNINNLTNELNTSRGGIGGINAPHIYHQRINSTIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58171</accession>
    <name>Y761_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Uncharacterized metal-dependent hydrolase MJ0761</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0761</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. TatD-type hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64395">
        <property type="entry name" value="A64395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870266.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58171"/>
    <dbReference type="SMR" id="Q58171"/>
    <dbReference type="STRING" id="243232.MJ_0761"/>
    <dbReference type="EnsemblBacteria" id="AAB98752">
        <property type="protein sequence ID" value="AAB98752"/>
        <property type="gene ID" value="MJ_0761"/>
    </dbReference>
    <dbReference type="GeneID" id="1451638"/>
    <dbReference type="KEGG" id="mja:MJ_0761"/>
    <dbReference type="eggNOG" id="arCOG00892">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061552_1_0_2"/>
    <dbReference type="InParanoid" id="Q58171"/>
    <dbReference type="OMA" id="HPMGYPK"/>
    <dbReference type="OrthoDB" id="44748at2157"/>
    <dbReference type="PhylomeDB" id="Q58171"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="PTHR42658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000202011" description="Uncharacterized metal-dependent hydrolase MJ0761">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0AFQ7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="251" mass="28838" checksum="ADE2B8F498D57970" modified="1996-11-01" version="1">MIDAHTHLDVRSFEDLEKMALSGIETIITCAHDPYKMSTPEVYLDHWDRLINLEVKRGEMAGVEVKVAVGVHPMGYPKNWEVLIKKLPEFLDNENVVAIGETGLHYLTEDEKNLLREQLYLAKDYNMPIIIHTPEKNKKEALIEILKILDEVKIKDSLVMIDHINKETVDLIDRDVYVGLTVQPSMKLTHEEAAEIIKNYNKKFILSSDLGSLKADIYALPRTKLYMKNIGVDEEKIIASVYKNAKGFYRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58214</accession>
    <name>Y804_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0804</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0804</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64400">
        <property type="entry name" value="D64400"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58214"/>
    <dbReference type="SMR" id="Q58214"/>
    <dbReference type="STRING" id="243232.MJ_0804"/>
    <dbReference type="EnsemblBacteria" id="AAB98804">
        <property type="protein sequence ID" value="AAB98804"/>
        <property type="gene ID" value="MJ_0804"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0804"/>
    <dbReference type="eggNOG" id="arCOG00955">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061501_0_0_2"/>
    <dbReference type="InParanoid" id="Q58214"/>
    <dbReference type="OMA" id="CIFCYSN"/>
    <dbReference type="PhylomeDB" id="Q58214"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000107054" description="Uncharacterized protein MJ0804">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="36"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="286" mass="32920" checksum="CACC47C3A3B4E63A" modified="1998-12-15" version="2">MIVLRNEICDKLENIAKILKFVRHCIGCEGINLEIENPQHHPSIELTQKCNLNCIYCYSRLKTVKRGIYGNLEEAETVTISQYGEPLLDLEGVKKAIEFCKDLGLRVDLQTNGTLLNEEIIKELKDLGLDLIMISLSSFSREKYKLLTGKDYFNRVLNNIKIASKYLHTIVRSIYIPGFNDNELLNLAKELNNYADEIMVHQLISYKENENLLKNAGIDLNNLGRIRDLLLIVDEMQKNAPKINVTIKGCLLVQLKEMDGFILNNITYDVFSEVPDIKREHRPLPW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58216</accession>
    <name>Y806_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized peptidase MJ0806</fullName>
            <ecNumber>3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0806</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64400">
        <property type="entry name" value="F64400"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58216"/>
    <dbReference type="SMR" id="Q58216"/>
    <dbReference type="STRING" id="243232.MJ_0806"/>
    <dbReference type="EnsemblBacteria" id="AAB98806">
        <property type="protein sequence ID" value="AAB98806"/>
        <property type="gene ID" value="MJ_0806"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0806"/>
    <dbReference type="eggNOG" id="arCOG01000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017266_4_2_2"/>
    <dbReference type="InParanoid" id="Q58216"/>
    <dbReference type="OMA" id="YCSDRTR"/>
    <dbReference type="PhylomeDB" id="Q58216"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_NTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000587">
        <property type="entry name" value="Creatinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="SSF53092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="SSF55920"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000185104" description="Uncharacterized peptidase MJ0806">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="312"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="347" mass="40549" checksum="60A4AA7224217C4D" modified="1996-11-01" version="1">MQKTIGDIMNNRIERFLKYMESEGIKKAVILKKENINYFLGKYFMSFSVLVFEEQPYLYVGKLDKDYAEEHFNFLEIREFKSWEEIFKGCDGVEKELSIGYLKYIDKEYKIISDKIKEMRMIKDKEEIKLIKKAAEISDKAINWVLNNLDEVKNLTEYELVAEIEYIMKKHGSIKPAFDSIVVSGKKTSFPHALPTKDKIADILLVDIGAVYEGYCSDITRTFLLKDDEEMKKIYNLVYEAKKVAEEHLKEGISAKQIDNIVREFFNDYKELFIHSLGHGVGLEVHEEPRLSNKLKDDEDIILKEGMVVTIEPGLYLKDKFGVRIEDLYLVKKNGFEKLSKAEISEY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58222</accession>
    <name>Y812_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0812</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0812</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64401">
        <property type="entry name" value="D64401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870323.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58222"/>
    <dbReference type="SMR" id="Q58222"/>
    <dbReference type="STRING" id="243232.MJ_0812"/>
    <dbReference type="EnsemblBacteria" id="AAB98811">
        <property type="protein sequence ID" value="AAB98811"/>
        <property type="gene ID" value="MJ_0812"/>
    </dbReference>
    <dbReference type="GeneID" id="1451695"/>
    <dbReference type="KEGG" id="mja:MJ_0812"/>
    <dbReference type="eggNOG" id="arCOG00436">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018678_0_1_2"/>
    <dbReference type="InParanoid" id="Q58222"/>
    <dbReference type="OMA" id="TRFTEPD"/>
    <dbReference type="OrthoDB" id="43030at2157"/>
    <dbReference type="PhylomeDB" id="Q58222"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045427">
        <property type="entry name" value="MoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041538">
        <property type="entry name" value="RavA-like_AAA_lid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17868">
        <property type="entry name" value="AAA_lid_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20030">
        <property type="entry name" value="bpMoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107060" description="Uncharacterized protein MJ0812">
        <location>
            <begin position="1"/>
            <end position="373"/>
        </location>
    </feature>
    <sequence length="373" mass="43183" checksum="D7168B1717DDB6AD" modified="1997-11-01" version="1">MLEKIREELNSYFLERREEIDIALTSILANEHTVFLGNPGVAKSQLIRAIASHINANYFEKLITRFTTEDELFGPLSIKELKDNDRFVRKTSGYLPTAEIAFLDEVFKANSSILNALLSIINERIYHNGDRIEKVPLISLFGASNELPEENELLAFYDRFLFRKVVRGIRSYENLSKLIDLEEEYKPKTIIDVEDVKKMQNEALKVDISNIKDDLIKIKLSLESEGIRISDRRFKKSVKAVKCFAYLNGKEKADENDLDILRHIYWNEPDEFYKVSVEIFKISNHFAGFALEQREILDSLMNEIKKINKDRIKLGGIEYRKCLEILGKLNSMSITLKDVKNKAIEANKPYELVEDVLKEVEGFKKYVEGLLKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58225</accession>
    <name>MFND_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tyramine--L-glutamate ligase</fullName>
            <ecNumber evidence="1">6.3.4.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mfnD</name>
        <name type="ordered locus">MJ0815</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of an amide bond between tyramine and the gamma carboxy group of L-glutamate. The enzyme also accepts phenylethylamine in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-glutamate + tyramine = ADP + gamma-L-glutamyltyramine + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43544"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83425"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.24" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G64401"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58225"/>
    <dbReference type="SMR" id="Q58225"/>
    <dbReference type="STRING" id="243232.MJ_0815"/>
    <dbReference type="EnsemblBacteria" id="AAB98814">
        <property type="protein sequence ID" value="AAB98814"/>
        <property type="gene ID" value="MJ_0815"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0815"/>
    <dbReference type="eggNOG" id="arCOG01592">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059501_1_1_2"/>
    <dbReference type="InParanoid" id="Q58225"/>
    <dbReference type="OMA" id="DVNPRPT"/>
    <dbReference type="PhylomeDB" id="Q58225"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024710">
        <property type="entry name" value="MfnD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040803">
        <property type="entry name" value="MfnD_preATP-grasp"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18301">
        <property type="entry name" value="preATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016766">
        <property type="entry name" value="UCP016766_ATPgrasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000013998" description="Tyramine--L-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="89"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="115"/>
            <end position="192"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="C7P8V7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <sequence length="308" mass="35061" checksum="08C458E046749061" modified="1996-11-01" version="1">MLLLQSILITKIMVIQLILFFEYALASGFEDKNILKEGKMMFDTLLKQFLEIDKVISLLYKDFVDNYIDFKNLEIVKIKKENEIENKLKSLLKSENIDYALVVAPEDEDILYNLTKIIESYPVKNLGCSSEAIKIAGNKYLTYLAIKDAVKTPKTFPPKKYVVKKIDSCGGKFNLFDENFLIQEFIDGENLSVSLIVGKKIHPLSLNRQYIDKRGFVGGEVNINHKLKDKIFNEAIKAVKCINGLNGYVGVDVIVNNDGIYIIEINPRITTTIYGLKTNPSLAELLIKNANNEELKFKVKGEKFTIDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58241</accession>
    <name>Y831_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0831</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0831</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0977.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="G64403">
        <property type="entry name" value="G64403"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870342.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6TVV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-432"/>
    </dbReference>
    <dbReference type="PDBsum" id="6TVV"/>
    <dbReference type="AlphaFoldDB" id="Q58241"/>
    <dbReference type="SMR" id="Q58241"/>
    <dbReference type="STRING" id="243232.MJ_0831"/>
    <dbReference type="PRIDE" id="Q58241"/>
    <dbReference type="EnsemblBacteria" id="AAB98830">
        <property type="protein sequence ID" value="AAB98830"/>
        <property type="gene ID" value="MJ_0831"/>
    </dbReference>
    <dbReference type="GeneID" id="1451714"/>
    <dbReference type="KEGG" id="mja:MJ_0831"/>
    <dbReference type="eggNOG" id="arCOG00427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042622_0_0_2"/>
    <dbReference type="InParanoid" id="Q58241"/>
    <dbReference type="OMA" id="AVGDMQE"/>
    <dbReference type="OrthoDB" id="45290at2157"/>
    <dbReference type="PhylomeDB" id="Q58241"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
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    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107067" description="Uncharacterized protein MJ0831">
        <location>
            <begin position="1"/>
            <end position="432"/>
        </location>
    </feature>
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        <location>
            <begin position="4"/>
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        </location>
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        <location>
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        </location>
    </feature>
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        <location>
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        <location>
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    </feature>
    <feature type="strand" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="367"/>
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    </feature>
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            <begin position="376"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="385"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="390"/>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="402"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="406"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="412"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="425"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="6TVV"/>
        </source>
    </evidence>
    <sequence length="432" mass="48835" checksum="0EB95D188D2B41F3" modified="1996-11-01" version="1">MMEKLKEIEKVTKAIKEKILNHYGYIRVITHHDTDGLSSGGILAKMLMRTNKLFHLTVVEHLSKEVIEKLAKENEVNKPLFIFADMGSGQIEEIIKHNFNAIILDHHPPVIKDSFINENIIQLNPHIFGVDGSREITASGVCYLVAREFGYYDLSVLAIVGIIGDMQYNPLLGLNKFIVNEAREYRYVKIMNDIVYNIYDVEIYKAIAYCTKPYIPDLASEGKAFKFLKDIGIDPNKKQLDDTDKKKLLSAIIFKYPKIENLLIDRYLIEHKVRDAFLLSEMLNAVGRNGLFAVGIGICLEDDECIKIGNQILWEYKKNLINELKSVKLKKLNNIYYFEGKKGMIGIIASILVDDKPVIGYHIEGDIAKFSARGNRDLVNRGLNLSVAMAVAKEFGGNGGGHDVASGAVVSKDKVQEFLKRVDEIIGEQLRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58261</accession>
    <name>MTRA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrahydromethanopterin S-methyltransferase subunit A</fullName>
            <ecNumber evidence="1">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtrA</name>
        <name type="ordered locus">MJ0851</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
        </cofactor>
        <text evidence="1">Binds 1 5-hydroxybenzimidazolylcobamide group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtrA family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98856" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98856.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64406">
        <property type="entry name" value="C64406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496640.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="5L8X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B=1-170"/>
    </dbReference>
    <dbReference type="PDB" id="6ZW0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.05 A"/>
        <property type="chains" value="C/D=140-169"/>
    </dbReference>
    <dbReference type="PDBsum" id="5L8X"/>
    <dbReference type="PDBsum" id="6ZW0"/>
    <dbReference type="AlphaFoldDB" id="Q58261"/>
    <dbReference type="SMR" id="Q58261"/>
    <dbReference type="IntAct" id="Q58261">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="Q58261"/>
    <dbReference type="STRING" id="243232.MJ_0851"/>
    <dbReference type="DNASU" id="1451739"/>
    <dbReference type="EnsemblBacteria" id="AAB98856">
        <property type="protein sequence ID" value="AAB98856"/>
        <property type="gene ID" value="MJ_0851"/>
    </dbReference>
    <dbReference type="GeneID" id="1451739"/>
    <dbReference type="KEGG" id="mja:MJ_0851"/>
    <dbReference type="eggNOG" id="arCOG03221">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100863_0_0_2"/>
    <dbReference type="InParanoid" id="Q58261"/>
    <dbReference type="OMA" id="EVKGHIT"/>
    <dbReference type="OrthoDB" id="89197at2157"/>
    <dbReference type="PhylomeDB" id="Q58261"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01093">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030688">
        <property type="entry name" value="MeTrfase_MtrA/MtxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005778">
        <property type="entry name" value="MtrA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04208">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500207">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009452">
        <property type="entry name" value="MtrA_MtxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01111">
        <property type="entry name" value="mtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147503" description="Tetrahydromethanopterin S-methyltransferase subunit A">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
            <note>cofactor</note>
        </ligand>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="26"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="38"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="74"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="103"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="116"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="120"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="132"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="165"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5L8X"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="6ZW0"/>
        </source>
    </evidence>
    <sequence length="241" mass="25880" checksum="155EAE6418E09B8D" modified="2005-03-29" version="2">MANKREPAPGWPIVSGEYVVGNPESCVGVVTLGSHGLEQACIDAGAAIAGPCHTENLGIEKVVANYISNPNIRFMILCGSEVQGHITGQCFKALWENGIGDDGGIIGAKGAIPFLENVNKEAVERFRRQIVEVVDLIDCEDIGKITQAIKECLSKDPGAIDEDPFIIELEGGKGGGEEEEGVIKPITPEMAIIESRMRLIGNEMCYNGLLAKWQAGYYNGKIQGIATGLFLMLLIMGILMF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58281</accession>
    <name>Y871_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0871</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0871</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0880, MJ1556 and MJ1589.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64408">
        <property type="entry name" value="G64408"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58281"/>
    <dbReference type="STRING" id="243232.MJ_0871"/>
    <dbReference type="EnsemblBacteria" id="AAB98877">
        <property type="protein sequence ID" value="AAB98877"/>
        <property type="gene ID" value="MJ_0871"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0871"/>
    <dbReference type="eggNOG" id="arCOG00360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048086_2_0_2"/>
    <dbReference type="InParanoid" id="Q58281"/>
    <dbReference type="OMA" id="YGIWGAE"/>
    <dbReference type="OrthoDB" id="68115at2157"/>
    <dbReference type="PhylomeDB" id="Q58281"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="PTHR38139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107086" description="Uncharacterized protein MJ0871">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="317" mass="35128" checksum="4F6346E8A10BCB5D" modified="1996-11-01" version="1">MIVVDYITPLMESMKISAYYTIRISIIVLTTVFIVNYIMSTGIMKKLSNMLSPILRRLKVNPLSISSTLACFFSPTVGYSILAEGLKENKVNEREVIGASLANSFPSVLSHTFTFFIPVVVPILGHTGVLYVLIRLGVALAKTIIGFLYLSIISEDYSFEMPEINKLNKKENAKKSFKSTIRFAKRLIPIMFFMMTLVLYLSKIGFFDYVEKFVQPITNLLNLNPNVGILALTEIMNVQAAIVMAGGFLNEGILSSKEVLIGLIIGNVLTFSTRYVKHSLPLHVSLFGAKLGTKIVMVNAAITLLLDIFIIAGLLLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58337</accession>
    <name>GCH1L_METJA</name>
    <protein>
        <recommendedName>
            <fullName>GTP cyclohydrolase 1 type 2 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0927</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2013" name="Acta Crystallogr. F Struct. Biol. Commun." volume="69" first="80" last="82">
            <title>Crystallization and preliminary X-ray diffraction analysis of the Nif3-family protein MJ0927 from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Kuan S.M."/>
                <person name="Chen H.C."/>
                <person name="Huang C.H."/>
                <person name="Chang C.H."/>
                <person name="Chen S.C."/>
                <person name="Yang C.S."/>
                <person name="Chen Y."/>
            </authorList>
            <dbReference type="PubMed" id="23295494"/>
            <dbReference type="DOI" id="10.1107/S1744309112049408"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2014" name="Biomed. Res. Int." volume="2014" first="171263" last="171263">
            <title>Crystal structure of a conserved hypothetical protein MJ0927 from Methanocaldococcus jannaschii reveals a novel quaternary assembly in the Nif3 family.</title>
            <authorList>
                <person name="Chen S.C."/>
                <person name="Huang C.H."/>
                <person name="Yang C.S."/>
                <person name="Kuan S.M."/>
                <person name="Lin C.T."/>
                <person name="Chou S.H."/>
                <person name="Chen Y."/>
            </authorList>
            <dbReference type="PubMed" id="25243119"/>
            <dbReference type="DOI" id="10.1155/2014/171263"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.47 ANGSTROMS)</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <comment type="function">
        <text evidence="2">DNA-binding protein exhibiting the ability to bind to both single-stranded and double-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homohexamer; trimer of dimers, that forms a hollow cage-like architecture.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98929" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98929.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64415">
        <property type="entry name" value="G64415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496667.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4IWG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.47 A"/>
        <property type="chains" value="A/B/C=1-244"/>
    </dbReference>
    <dbReference type="PDB" id="4IWM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-244"/>
    </dbReference>
    <dbReference type="PDBsum" id="4IWG"/>
    <dbReference type="PDBsum" id="4IWM"/>
    <dbReference type="AlphaFoldDB" id="Q58337"/>
    <dbReference type="SMR" id="Q58337"/>
    <dbReference type="MINT" id="Q58337"/>
    <dbReference type="STRING" id="243232.MJ_0927"/>
    <dbReference type="EnsemblBacteria" id="AAB98929">
        <property type="protein sequence ID" value="AAB98929"/>
        <property type="gene ID" value="MJ_0927"/>
    </dbReference>
    <dbReference type="GeneID" id="1451816"/>
    <dbReference type="KEGG" id="mja:MJ_0927"/>
    <dbReference type="eggNOG" id="arCOG04454">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037423_2_0_2"/>
    <dbReference type="InParanoid" id="Q58337"/>
    <dbReference type="OMA" id="THHSKIL"/>
    <dbReference type="OrthoDB" id="40682at2157"/>
    <dbReference type="PhylomeDB" id="Q58337"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="PTHR13799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="SSF102705"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147348" description="GTP cyclohydrolase 1 type 2 homolog">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="3"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="16"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="26"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="39"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="48"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="60"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="78"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="93"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="99"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="108"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="119"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="139"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="155"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="167"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="178"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="189"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="210"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="216"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="237"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0AFP6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="25243119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4IWG"/>
        </source>
    </evidence>
    <sequence length="244" mass="27363" checksum="BC42E5E29AAE399F" modified="2002-05-15" version="2">MKAKEIIEFIETFAPKDLAIEGDNIGLQVGDNLDKEIKKLGIALDPSLSVIKKAEKEGVDFLFTHHPLLKDPIRNFTGVIYKKLKILMENDIILYSAHTNLDICKNGLNDALAELYNLENPKPLYDNGLGRVGIFKGSFEEFLEITKKYIHKNPIVVKSKEVDDNFKLAVLSGYGLSQSSIKYVAEKADVYLSGDLTHHSKILAEELGLVVVDATHYSTEVFGLKKFKEFLSSNLDLEIISLDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58369</accession>
    <name>Y959_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized aminotransferase MJ0959</fullName>
            <ecNumber>2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0959</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64419">
        <property type="entry name" value="G64419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870473.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58369"/>
    <dbReference type="SMR" id="Q58369"/>
    <dbReference type="STRING" id="243232.MJ_0959"/>
    <dbReference type="EnsemblBacteria" id="AAB98961">
        <property type="protein sequence ID" value="AAB98961"/>
        <property type="gene ID" value="MJ_0959"/>
    </dbReference>
    <dbReference type="GeneID" id="1451857"/>
    <dbReference type="KEGG" id="mja:MJ_0959"/>
    <dbReference type="eggNOG" id="arCOG00082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027686_1_1_2"/>
    <dbReference type="InParanoid" id="Q58369"/>
    <dbReference type="OMA" id="KNWLPIM"/>
    <dbReference type="OrthoDB" id="20398at2157"/>
    <dbReference type="PhylomeDB" id="Q58369"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-15919"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024169">
        <property type="entry name" value="SP_NH2Trfase/AEP_transaminase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000524">
        <property type="entry name" value="SPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150343" description="Uncharacterized aminotransferase MJ0959">
        <location>
            <begin position="1"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="385" mass="42413" checksum="7DA5410050D3FB1A" modified="1996-11-01" version="1">MKIDAVKKLLMIPGPTMVPPEVLNAMALPVIGHRTKDYSNLLEDTIEKLKKVFITENDTFLITGSGTAAMDMAISNIIKRGDKVLNIVTGNFGERFANIVKAYKGEAIRLDVEWGDMAEPEAVKEILDKYDDIKAVTVVHNETSTGARNPIKEIGEVVKDYDALYIVDTVSSLGGDYVNVDKFHIDICVTGSQKCLAAPPGLAAITVSEKAWEVIKKNDDKVGFYLDLLAYKKYYEEKKQTPYTPSVNLTYALNVALDLVLEEGIENRVKRHERLAKATRAGLEAMGIELFAKERARSVTVTSAKYPEGIEDSKFRGILSNKYNIVVAGGQKHLAGKIFRIGHMGICGEKEVLATLACVELALKELGFEVKESGVEVAKEVLLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58381</accession>
    <name>Y971_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0971</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0971</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To S.solfataricus C04034.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64421">
        <property type="entry name" value="C64421"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58381"/>
    <dbReference type="STRING" id="243232.MJ_0971"/>
    <dbReference type="EnsemblBacteria" id="AAB98976">
        <property type="protein sequence ID" value="AAB98976"/>
        <property type="gene ID" value="MJ_0971"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0971"/>
    <dbReference type="eggNOG" id="arCOG04064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042134_1_0_2"/>
    <dbReference type="InParanoid" id="Q58381"/>
    <dbReference type="OMA" id="TIPLWYG"/>
    <dbReference type="PhylomeDB" id="Q58381"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PTHR13325"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13180">
        <property type="entry name" value="PDZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="SSF50156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107124" description="Uncharacterized protein MJ0971">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="365" mass="41082" checksum="F1608E3149691942" modified="1996-11-01" version="1">MNMDTSKVILIVAIIIWIILYSIRDSINLKTYGGIFGILRTKLGLKTIEKLGKYKIWQKIGIISIPICVILGFFMLLNIIDMSIRLLSGTLPKEAAKPVVFLFGDVIPWIPGIIALLIAISVHELAHGIFAKSFGIKVKSSGILLLLGLPLGAFVELGDEFKTADKKIRGAIASAGPLANLIIFLTSIPLLSFSYTLPTELKIIDVKEPASEFLQKGDIIYEINGKKINSLEDFKEFAKTIEPKKEYEIKILRDNKILTYKIVSSNEGKLGIMVSPTKNTALFINTIYWTYWFNFLLALFNLLPAMPLDGFHVWNAFPELLKERKNRFISKVGQILELFINEKTLGSITLLVWWVILGSILYSMW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58382</accession>
    <name>Y972_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0056 membrane protein MJ0972</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0972</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64421">
        <property type="entry name" value="D64421"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58382"/>
    <dbReference type="STRING" id="243232.MJ_0972"/>
    <dbReference type="EnsemblBacteria" id="AAB98977">
        <property type="protein sequence ID" value="AAB98977"/>
        <property type="gene ID" value="MJ_0972"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0972"/>
    <dbReference type="eggNOG" id="arCOG01997">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079909_1_0_2"/>
    <dbReference type="InParanoid" id="Q58382"/>
    <dbReference type="OMA" id="MMAIVML"/>
    <dbReference type="PhylomeDB" id="Q58382"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="PTHR33508"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00427">
        <property type="entry name" value="TIGR00427"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000156917" description="UPF0056 membrane protein MJ0972">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="228" mass="25360" checksum="D259B7E8C5F38D6E" modified="1996-11-01" version="1">MVGYSGEHFIFNVVKYMDILNFYIYGFVSLFITIDPIGLIPIVHSLTYPYPKEQRIRIIKKAIISSTVVLLLFALFGNYIFGYFGITIDAFRVAGGILLFKIAWDMLHAEIPKTKHKPDERLDLEDIDSIVYVPLAIPLISGPGAITTTMILISKTQSILEKGVVVLSILSAMLVSGIILSLTDFIIRRVNIYGINAFVRIMGLLLVAISVQIIFTGIVGLYNSISVQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58385</accession>
    <name>VAPB2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative antitoxin MJ0975</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vapB2</name>
        <name type="ordered locus">MJ0975</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possibly the antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is VapC2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0165 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98981" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98981.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64421">
        <property type="entry name" value="G64421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496686.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58385"/>
    <dbReference type="STRING" id="243232.MJ_0975"/>
    <dbReference type="EnsemblBacteria" id="AAB98981">
        <property type="protein sequence ID" value="AAB98981"/>
        <property type="gene ID" value="MJ_0975"/>
    </dbReference>
    <dbReference type="GeneID" id="1451873"/>
    <dbReference type="KEGG" id="mja:MJ_0975"/>
    <dbReference type="eggNOG" id="arCOG08303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200885_3_1_2"/>
    <dbReference type="InParanoid" id="Q58385"/>
    <dbReference type="OMA" id="CEVEIKI"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008203">
        <property type="entry name" value="AF2212-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024069">
        <property type="entry name" value="AF2212-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01954">
        <property type="entry name" value="DUF104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000156858" description="Putative antitoxin MJ0975">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="64" mass="7214" checksum="C10D5F4CA7C7094F" modified="2001-06-20" version="2">MEIVVDAIYEKGVLKLKKSINLPEGCEVEIKIIPKKISEKTFGILKLSDKEIKEILEEIENGGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58401</accession>
    <name>HEM4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative uroporphyrinogen-III synthase</fullName>
            <shortName>UROS</shortName>
            <ecNumber>4.2.1.75</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Hydroxymethylbilane hydrolyase [cyclizing]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Uroporphyrinogen-III cosynthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hemD</name>
        <name type="ordered locus">MJ0994</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>hydroxymethylbilane = H2O + uroporphyrinogen III</text>
            <dbReference type="Rhea" id="RHEA:18965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="EC" id="4.2.1.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 3/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the uroporphyrinogen-III synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.75"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64424">
        <property type="entry name" value="B64424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870508.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58401"/>
    <dbReference type="SMR" id="Q58401"/>
    <dbReference type="STRING" id="243232.MJ_0994"/>
    <dbReference type="EnsemblBacteria" id="AAB98999">
        <property type="protein sequence ID" value="AAB98999"/>
        <property type="gene ID" value="MJ_0994"/>
    </dbReference>
    <dbReference type="GeneID" id="1451892"/>
    <dbReference type="KEGG" id="mja:MJ_0994"/>
    <dbReference type="eggNOG" id="arCOG02048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_9_5_2"/>
    <dbReference type="InParanoid" id="Q58401"/>
    <dbReference type="OMA" id="VWAVGKT"/>
    <dbReference type="OrthoDB" id="106625at2157"/>
    <dbReference type="PhylomeDB" id="Q58401"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00320"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004852">
        <property type="term" value="F:uroporphyrinogen-III synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006780">
        <property type="term" value="P:uroporphyrinogen III biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06578">
        <property type="entry name" value="HemD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10090">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036108">
        <property type="entry name" value="4pyrrol_syn_uPrphyn_synt_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003754">
        <property type="entry name" value="4pyrrol_synth_uPrphyn_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039793">
        <property type="entry name" value="UROS/Hem4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38042">
        <property type="entry name" value="PTHR38042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02602">
        <property type="entry name" value="HEM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69618">
        <property type="entry name" value="SSF69618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135249" description="Putative uroporphyrinogen-III synthase">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="240" mass="27614" checksum="F0AA2E8CCEF9BAE8" modified="1996-11-01" version="1">MKVVITRPKERADVFASLLKKEGFEPIIFPTLEIVYNKDLDVNLDSYDWIAFTSPSGVIGLYNILTENERENVKNKKIAVIGEKTAKTFKKYFGRDPDIMPNEYTAESLLREIKKVSKEEEKFLIPTTPSTRDVLKNNLNADLLFVYKSAEPENLKEDIKKLKELIAKDKFILTFTSGLTAKNFFKYVDDEFAEIIKDNYIVAIGPITAKVIEKFGFKPLIPKVYTIEGMLEVIRTLKER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58402</accession>
    <name>Y995_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0995</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0995</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64424">
        <property type="entry name" value="C64424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870509.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58402"/>
    <dbReference type="STRING" id="243232.MJ_0995"/>
    <dbReference type="EnsemblBacteria" id="AAB99000">
        <property type="protein sequence ID" value="AAB99000"/>
        <property type="gene ID" value="MJ_0995"/>
    </dbReference>
    <dbReference type="GeneID" id="1451893"/>
    <dbReference type="KEGG" id="mja:MJ_0995"/>
    <dbReference type="eggNOG" id="arCOG06579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264574_0_0_2"/>
    <dbReference type="OMA" id="VYVYLEF"/>
    <dbReference type="OrthoDB" id="81436at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107136" description="Uncharacterized protein MJ0995">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="227" mass="26360" checksum="F9D84BB3E76BB568" modified="1996-11-01" version="1">MYGDYMKYMKKIVLFLIINILPILILGLYLYANIGGAEDVKEVIENSPFKEFTYIDHKTLMMLKNDVNLKNMPEFYKESIILINGIYIGNHGSFGIKIPLGFLIKYIPIDNFKYYNGVLIKNLNEDDLGKAEMNDLVNTIPPNYKDVLIYRENYTIGIYYDLNSNKTYLIEVFRKPNNQEIDTEKLRNELLQKTNAVDCNVVDMGDKVYVYLEFNGIDLNLINNGIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58436</accession>
    <name>COAD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="1">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">coaD</name>
        <name type="ordered locus">MJ1030</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic CoaD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64428">
        <property type="entry name" value="E64428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870543.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58436"/>
    <dbReference type="SMR" id="Q58436"/>
    <dbReference type="STRING" id="243232.MJ_1030"/>
    <dbReference type="PRIDE" id="Q58436"/>
    <dbReference type="EnsemblBacteria" id="AAB99034">
        <property type="protein sequence ID" value="AAB99034"/>
        <property type="gene ID" value="MJ_1030"/>
    </dbReference>
    <dbReference type="GeneID" id="1451927"/>
    <dbReference type="KEGG" id="mja:MJ_1030"/>
    <dbReference type="eggNOG" id="arCOG01223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035272_5_0_2"/>
    <dbReference type="InParanoid" id="Q58436"/>
    <dbReference type="OMA" id="FDTLHSG"/>
    <dbReference type="OrthoDB" id="93412at2157"/>
    <dbReference type="PhylomeDB" id="Q58436"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004140">
        <property type="term" value="F:dephospho-CoA kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00647">
        <property type="entry name" value="PPAT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156320" description="Phosphopantetheine adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00647"/>
        </source>
    </evidence>
    <sequence length="147" mass="16669" checksum="999471C211B1B1C9" modified="1996-11-01" version="1">MKVVVGGTFDILHRGHKELLKFASSLGKLTIGITSDEFAKKYKTHKINDLKTRIENLKKFLDSIKADYEIKVINDAYGDAITEDYDIIVVTQETLKNAEKINKIRESKGLKPLKIVIFKPILAEDGKPISTTRIRKGEIDEEGRIIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58440</accession>
    <name>SRP19_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle 19 kDa protein</fullName>
            <shortName evidence="1">SRP19</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">srp19</name>
        <name type="ordered locus">MJ1034</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SRP19 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64429">
        <property type="entry name" value="A64429"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870547.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1L9A">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A=1-87"/>
    </dbReference>
    <dbReference type="PDB" id="1LNG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-87"/>
    </dbReference>
    <dbReference type="PDB" id="2V3C">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-87"/>
    </dbReference>
    <dbReference type="PDB" id="3NDB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=1-87"/>
    </dbReference>
    <dbReference type="PDB" id="4XCO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B=1-87"/>
    </dbReference>
    <dbReference type="PDBsum" id="1L9A"/>
    <dbReference type="PDBsum" id="1LNG"/>
    <dbReference type="PDBsum" id="2V3C"/>
    <dbReference type="PDBsum" id="3NDB"/>
    <dbReference type="PDBsum" id="4XCO"/>
    <dbReference type="AlphaFoldDB" id="Q58440"/>
    <dbReference type="SMR" id="Q58440"/>
    <dbReference type="DIP" id="DIP-46443N"/>
    <dbReference type="IntAct" id="Q58440">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1034"/>
    <dbReference type="EnsemblBacteria" id="AAB99038">
        <property type="protein sequence ID" value="AAB99038"/>
        <property type="gene ID" value="MJ_1034"/>
    </dbReference>
    <dbReference type="GeneID" id="1451931"/>
    <dbReference type="KEGG" id="mja:MJ_1034"/>
    <dbReference type="eggNOG" id="arCOG01217">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_169299_1_0_2"/>
    <dbReference type="InParanoid" id="Q58440"/>
    <dbReference type="OMA" id="VIWPAYL"/>
    <dbReference type="OrthoDB" id="121943at2157"/>
    <dbReference type="PhylomeDB" id="Q58440"/>
    <dbReference type="EvolutionaryTrace" id="Q58440"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00305">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022938">
        <property type="entry name" value="SRP19_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="PTHR17453"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SSF69695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0733">Signal recognition particle</keyword>
    <feature type="chain" id="PRO_0000135217" description="Signal recognition particle 19 kDa protein">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="5"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="14"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="26"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="63"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00305"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="1LNG"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3NDB"/>
        </source>
    </evidence>
    <sequence length="87" mass="10352" checksum="0BF272957E69CBE8" modified="1997-11-01" version="1">MIIWPSYIDKKKSRREGRKVPEELAIEKPSLKDIEKALKKLGLEPKIYRDKRYPRQHWEICGCVEVDYKGNKLQLLKEICKIIKGKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58451</accession>
    <name>Y1051_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1051</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1051</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NodU/CmcH family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64431">
        <property type="entry name" value="B64431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870564.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58451"/>
    <dbReference type="SMR" id="Q58451"/>
    <dbReference type="STRING" id="243232.MJ_1051"/>
    <dbReference type="EnsemblBacteria" id="AAB99054">
        <property type="protein sequence ID" value="AAB99054"/>
        <property type="gene ID" value="MJ_1051"/>
    </dbReference>
    <dbReference type="GeneID" id="1451948"/>
    <dbReference type="KEGG" id="mja:MJ_1051"/>
    <dbReference type="eggNOG" id="arCOG01188">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014411_2_0_2"/>
    <dbReference type="InParanoid" id="Q58451"/>
    <dbReference type="OMA" id="RTEMDYL"/>
    <dbReference type="OrthoDB" id="34157at2157"/>
    <dbReference type="PhylomeDB" id="Q58451"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031730">
        <property type="entry name" value="Carbam_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038152">
        <property type="entry name" value="Carbam_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003696">
        <property type="entry name" value="Carbtransf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16861">
        <property type="entry name" value="Carbam_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02543">
        <property type="entry name" value="Carbam_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000207857" description="Uncharacterized protein MJ1051">
        <location>
            <begin position="1"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="513" mass="58662" checksum="C91414C702AE4A2B" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58507</accession>
    <name>Y1107_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1107</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1107</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the M.jannaschii MJ0023/MJ0349/MJ1072/MJ1074/MJ1107/MJECL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64438">
        <property type="entry name" value="B64438"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58507"/>
    <dbReference type="SMR" id="Q58507"/>
    <dbReference type="STRING" id="243232.MJ_1107"/>
    <dbReference type="PRIDE" id="Q58507"/>
    <dbReference type="EnsemblBacteria" id="AAB99111">
        <property type="protein sequence ID" value="AAB99111"/>
        <property type="gene ID" value="MJ_1107"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1107"/>
    <dbReference type="eggNOG" id="arCOG09652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1821019_0_0_2"/>
    <dbReference type="OMA" id="IILANYA"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107171" description="Uncharacterized protein MJ1107">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="149" mass="17603" checksum="7DC7EC894464EF50" modified="1997-11-01" version="1">MKFFVVFVMAVAYSKLYELIKNVKDEKEAEELCKIIEEFFEKQCKENVSKKFEEQKPVLKLELKEELRKELTTKEDLELIGEKILRYVDNKINQVIEKINQLDKKIDEGFYQLDKKVDTLKRDIIIIALIIILANYAPSIIGKILSFLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58538</accession>
    <name>Y1138_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1138</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1138</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64442">
        <property type="entry name" value="A64442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870649.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58538"/>
    <dbReference type="SMR" id="Q58538"/>
    <dbReference type="EnsemblBacteria" id="AAB99145">
        <property type="protein sequence ID" value="AAB99145"/>
        <property type="gene ID" value="MJ_1138"/>
    </dbReference>
    <dbReference type="GeneID" id="1452034"/>
    <dbReference type="KEGG" id="mja:MJ_1138"/>
    <dbReference type="eggNOG" id="arCOG03788">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1559485_0_0_2"/>
    <dbReference type="InParanoid" id="Q58538"/>
    <dbReference type="OMA" id="SGCLEQP"/>
    <dbReference type="OrthoDB" id="94168at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009269">
        <property type="term" value="P:response to desiccation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013990">
        <property type="entry name" value="WHy-dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00769">
        <property type="entry name" value="WHy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107185" description="Uncharacterized protein MJ1138">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="174" mass="18948" checksum="CC03739B5FDF5764" modified="1996-11-01" version="1">MKSVKKLLLLAFAVCLAVGFSGCLEQPKIEVVGQKIQKVDADNTKIEIQVLVDNPNPIGISIDKISFDIYALVGGDKIYLGHGEQSNIKITSGNTTFTLPVTISNKKLVEVALKEKSTKIPIEIKGDIAVNLFITKVNIPIDIQQEIDVSAIAKEEVLNQLNNLNPNQIQSIAQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58562</accession>
    <name>Y1162_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1162</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1162</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64445">
        <property type="entry name" value="A64445"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58562"/>
    <dbReference type="SMR" id="Q58562"/>
    <dbReference type="STRING" id="243232.MJ_1162"/>
    <dbReference type="EnsemblBacteria" id="AAB99164">
        <property type="protein sequence ID" value="AAB99164"/>
        <property type="gene ID" value="MJ_1162"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1162"/>
    <dbReference type="eggNOG" id="arCOG01145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041441_5_0_2"/>
    <dbReference type="InParanoid" id="Q58562"/>
    <dbReference type="OMA" id="AVPGNCD"/>
    <dbReference type="PhylomeDB" id="Q58562"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07392">
        <property type="entry name" value="MPP_PAE1087"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041733">
        <property type="entry name" value="PAE1087_MPP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107198" description="Uncharacterized protein MJ1162">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <sequence length="218" mass="24676" checksum="2F9D763416039EE4" modified="1996-11-01" version="1">MVKYMKIVGITDLHGKLPPAVREFKDFADVLVVCGDITHFGKGIEVIEKLAELSDYMEVLCVPGNCDTKEVIDELNSFKLNIDRKVKKIENINFVGIGGSNKTPFNTPNEYTEEEIYNKLINVVKNLKNIFLVSHAPPYNTMADIVDLDKDIHVGSKSIRKIIEDFNENIRFCACGHIHESRCIDKIGNTIVVNPSPKSYFVYDTKKNMVVLDDFNGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-18" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58563</accession>
    <name>Y1163_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0173 metal-dependent hydrolase MJ1163</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1163</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0173 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64445">
        <property type="entry name" value="B64445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870676.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58563"/>
    <dbReference type="SMR" id="Q58563"/>
    <dbReference type="STRING" id="243232.MJ_1163"/>
    <dbReference type="EnsemblBacteria" id="AAB99165">
        <property type="protein sequence ID" value="AAB99165"/>
        <property type="gene ID" value="MJ_1163"/>
    </dbReference>
    <dbReference type="GeneID" id="1452061"/>
    <dbReference type="KEGG" id="mja:MJ_1163"/>
    <dbReference type="eggNOG" id="arCOG00497">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070010_4_0_2"/>
    <dbReference type="InParanoid" id="Q58563"/>
    <dbReference type="OMA" id="WHGHSTW"/>
    <dbReference type="OrthoDB" id="76909at2157"/>
    <dbReference type="PhylomeDB" id="Q58563"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156393" description="UPF0173 metal-dependent hydrolase MJ1163">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <sequence length="217" mass="23516" checksum="A2FE84E38D9BC616" modified="1996-11-01" version="1">MITWYGHACFKVDNVLIDPFVPNPLCDLPYDEIMEGVEVIAVTHGHADHLGNAEELAKTYNVPVVTNHEISVYLSERGVCAEGMNIGGTIEINGAKLTMVKAEHSSDISPTISGGVAAGFIINDRVYHAGDTGLFGDMELIGEIYAPQIALLPIGGRYTMGIDEALVAIELIYPEIVIPMHYNTFPLIEVDVNEFVKKAEALGVEVIVPKIGEPLEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58565</accession>
    <name>FWDE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Ferredoxin-type protein FwdE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fwdE</name>
        <name type="ordered locus">MJ1165</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64445">
        <property type="entry name" value="D64445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870678.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58565"/>
    <dbReference type="STRING" id="243232.MJ_1165"/>
    <dbReference type="EnsemblBacteria" id="AAB99167">
        <property type="protein sequence ID" value="AAB99167"/>
        <property type="gene ID" value="MJ_1165"/>
    </dbReference>
    <dbReference type="GeneID" id="1452063"/>
    <dbReference type="KEGG" id="mja:MJ_1165"/>
    <dbReference type="eggNOG" id="arCOG04891">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145181_0_0_2"/>
    <dbReference type="OMA" id="LCASCIF"/>
    <dbReference type="OrthoDB" id="91206at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000159139" description="Ferredoxin-type protein FwdE">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="146" mass="16403" checksum="5BB26ED1CB2FDADD" modified="1996-11-01" version="1">MPEHILSGIKAIVAMKLRRKGLLQKEIAKIIKSDRSIVSHYLSGRYPKEKILNVAKIIEEMPPQYGAKFIHSLTDNKELAKNLIKELYGIKLFWDENSCIACGSCLGCAALTLDNFTVGIDEDTCHLCASCIFRCPTNSLKFIKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58588</accession>
    <name>Y1187_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1187</fullName>
            <ecNumber>3.2.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1187</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64448">
        <property type="entry name" value="C64448"/>
    </dbReference>
    <dbReference type="PDB" id="1T5J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A=2-301"/>
    </dbReference>
    <dbReference type="PDBsum" id="1T5J"/>
    <dbReference type="AlphaFoldDB" id="Q58588"/>
    <dbReference type="SMR" id="Q58588"/>
    <dbReference type="STRING" id="243232.MJ_1187"/>
    <dbReference type="EnsemblBacteria" id="AAB99191">
        <property type="protein sequence ID" value="AAB99191"/>
        <property type="gene ID" value="MJ_1187"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1187"/>
    <dbReference type="eggNOG" id="arCOG04448">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024566_7_0_2"/>
    <dbReference type="InParanoid" id="Q58588"/>
    <dbReference type="OMA" id="IPPSWEQ"/>
    <dbReference type="PhylomeDB" id="Q58588"/>
    <dbReference type="EvolutionaryTrace" id="Q58588"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="SSF101478"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000157289" description="Uncharacterized protein MJ1187">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="30"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="49"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="100"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="129"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="157"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="195"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="202"/>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="210"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="240"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="254"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="271"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="276"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="285"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="1T5J"/>
        </source>
    </evidence>
    <sequence length="301" mass="33568" checksum="525E5CE4D369C201" modified="1996-11-01" version="1">MVKMRDKILGSVFGAVIGDALGMPTENLTKEEIKKLYGFVDSYVEPKNYLAGKLNKGEWTDDTEQAICLIKSLTKEGIDIKKFANCLIAWKNKNPPDIGLTSLMAIDKLENNDYSGVDSSSCGAAMRIYPLGIVFHNNLKKLKEEVIKASKITHNNKTAIAGALAIAFFVSSALKDRKDFSLLDECYNYIKDIDEEFAKKLLEIKNFNNLDYIYDYFGTGVKTDEVVPSAIATYLLTDNFKEGMLKCINAGGDTDSLASMYGAMAGAYYGFKNIPKEWIDGLKNKEVIFELAERLYHLATE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58593</accession>
    <name>VHUB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Polyferredoxin protein VhuB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vhuB</name>
        <name type="ordered locus">MJ1193</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 10 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64448">
        <property type="entry name" value="H64448"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58593"/>
    <dbReference type="STRING" id="243232.MJ_1193"/>
    <dbReference type="PRIDE" id="Q58593"/>
    <dbReference type="EnsemblBacteria" id="AAB99195">
        <property type="protein sequence ID" value="AAB99195"/>
        <property type="gene ID" value="MJ_1193"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1193"/>
    <dbReference type="eggNOG" id="arCOG02181">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_693748_0_0_2"/>
    <dbReference type="InParanoid" id="Q58593"/>
    <dbReference type="OMA" id="ECIGCEK"/>
    <dbReference type="PhylomeDB" id="Q58593"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159146" description="Polyferredoxin protein VhuB">
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            <begin position="1"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
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            <begin position="4"/>
            <end position="33"/>
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            <begin position="26"/>
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            <begin position="64"/>
            <end position="93"/>
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            <begin position="133"/>
            <end position="162"/>
        </location>
    </feature>
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        <location>
            <begin position="162"/>
            <end position="191"/>
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    </feature>
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            <begin position="201"/>
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        </location>
    </feature>
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        <location>
            <begin position="231"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 9" evidence="2">
        <location>
            <begin position="292"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 10" evidence="2">
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            <begin position="334"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
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            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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        <source>
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    <evidence type="ECO:0000305" key="3"/>
    <sequence length="394" mass="43343" checksum="08C53D5F6C41D104" modified="1996-11-01" version="1">MRLMSITIQKDACLVCYACQAECPTKAIDIDSFKVCNLCMECVKVCPTGALVEEEIEVNGKKLKRVNYLAHKCEKCGQCAEACPIGIKKVDDDFPYSKGHCVLCQKCIDVCPIEIISLPGVIDKPKKEIKPPKEPIAVTDACVGCGICVPECPVNAITLENNKAVIDKSKCIYCSICAQTCPWNAIFVAGKIPKKRRKEVKKFEVNAEKCIYCLKCVEVCPGDMIKVDEENLIVIPPKSCPACKLCVNICPVDALDLEVKLSSPHPITDEGLVIVEEDFEVLKKCASVCPTEAIVVDEEKKEVRMCIVCGACTVACPTGALKLGKIEHNGKEYNRIEFSPYLCDKCGKCVEVCPMKTLRLSRGKLPLKGYCVMCLLCLSVAEKEKKGKKVLELR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58600</accession>
    <name>MTM7_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative type II methyltransferase M.MjaORF1200P</fullName>
            <shortName evidence="2">M.MjaORF1200P</shortName>
            <ecNumber>2.1.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cytosine-specific methyltransferase MJ1200</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1200</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">A putative methylase that probably protects DNA from cleavage by the MjaORF1200P endonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64449">
        <property type="entry name" value="G64449"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58600"/>
    <dbReference type="SMR" id="Q58600"/>
    <dbReference type="STRING" id="243232.MJ_1200"/>
    <dbReference type="REBASE" id="3906">
        <property type="entry name" value="M.MjaORF1200P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99203">
        <property type="protein sequence ID" value="AAB99203"/>
        <property type="gene ID" value="MJ_1200"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1200"/>
    <dbReference type="eggNOG" id="arCOG04157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006958_2_2_2"/>
    <dbReference type="InParanoid" id="Q58600"/>
    <dbReference type="OMA" id="EPFTGAN"/>
    <dbReference type="PhylomeDB" id="Q58600"/>
    <dbReference type="PRO" id="PR:Q58600"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000087917" description="Putative type II methyltransferase M.MjaORF1200P">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="domain" description="SAM-dependent MTase C5-type" evidence="1">
        <location>
            <begin position="5"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <sequence length="366" mass="42269" checksum="9D5C60CA603FBCFE" modified="1996-11-01" version="1">MVIMLKFIDLFCGCGGFSRGFVEEGFEPLVAIELNEDAAFSYALNFNGQIYEKIRPGEFKLKELKGYVGIYPFKFPFEEEDIKWLKRLGTLNEKTKKLSPVVINDDIREIHAIEIEKFIKNKKVDVIIGGPPCEGYTGANPKREKNPYDRLYKDETGRLVLEYIRIVGDLQPKIFVMENVPGIKEVRGAIIKEFREIGYEDVYFNTLRAEDYGNPSVRRRVFVSNIEINPEKTQPKTVIEAIGDLMYKGRDVPNHEFAALPARFRKRVHKLGWGDAFIYFKGANRRLGNYIRLHPLKLAETVMGKRFFIHPYEDRLLTPREQARLMSYPDYHLFAGGIRSCYNQIGESVPVALSRAIARVIKENLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58611</accession>
    <name>T1R2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative type I restriction enzyme MjaVIIIP endonuclease subunit</fullName>
            <shortName evidence="2">MjaVIIIP</shortName>
            <shortName evidence="3">R protein</shortName>
            <ecNumber evidence="1">3.1.21.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1214</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">The restriction (R) subunit of a type I restriction enzyme that recognizes 5'-GAYN(5)GTAA-3' and cleaves a random distance away. The R subunit is required for both endonuclease and ATPase activities but not for modification. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and magnesium as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HsdR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64451">
        <property type="entry name" value="E64451"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58611"/>
    <dbReference type="SMR" id="Q58611"/>
    <dbReference type="STRING" id="243232.MJ_1214"/>
    <dbReference type="REBASE" id="3903">
        <property type="entry name" value="MjaVIIIP"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99215">
        <property type="protein sequence ID" value="AAB99215"/>
        <property type="gene ID" value="MJ_1214"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1214"/>
    <dbReference type="eggNOG" id="arCOG00878">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005762_0_0_2"/>
    <dbReference type="InParanoid" id="Q58611"/>
    <dbReference type="OMA" id="KQYEAGM"/>
    <dbReference type="PhylomeDB" id="Q58611"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007409">
        <property type="entry name" value="Restrct_endonuc_type1_HsdR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004473">
        <property type="entry name" value="Restrct_endonuc_typeI_HsdR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040980">
        <property type="entry name" value="SWI2_SNF2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04313">
        <property type="entry name" value="HSDR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18766">
        <property type="entry name" value="SWI2_SNF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00348">
        <property type="entry name" value="hsdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000107217" description="Putative type I restriction enzyme MjaVIIIP endonuclease subunit">
        <location>
            <begin position="1"/>
            <end position="1018"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P08956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1018" mass="120993" checksum="97D383541BE128E2" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58639</accession>
    <name>Y1242_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ1242</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1242</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64455">
        <property type="entry name" value="A64455"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58639"/>
    <dbReference type="STRING" id="243232.MJ_1242"/>
    <dbReference type="EnsemblBacteria" id="AAB99247">
        <property type="protein sequence ID" value="AAB99247"/>
        <property type="gene ID" value="MJ_1242"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1242"/>
    <dbReference type="eggNOG" id="arCOG00185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_86_2_2"/>
    <dbReference type="InParanoid" id="Q58639"/>
    <dbReference type="OMA" id="AQADKMR"/>
    <dbReference type="PhylomeDB" id="Q58639"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019700">
        <property type="term" value="P:organic phosphonate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017669">
        <property type="entry name" value="Me_Coenz_M_Rdtase_A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03269">
        <property type="entry name" value="met_CoM_red_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093224" description="Uncharacterized ABC transporter ATP-binding protein MJ1242">
        <location>
            <begin position="1"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 1" evidence="1">
        <location>
            <begin position="9"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 2" evidence="1">
        <location>
            <begin position="288"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="325"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="539" mass="60707" checksum="26E5A58A897FE952" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q58647</accession>
    <name>GGGPS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="1">GGGP synthase</shortName>
            <shortName evidence="1">GGGPS</shortName>
            <ecNumber evidence="1">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1250</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2E,6E,10E)-geranylgeranyl diphosphate + sn-glycerol 1-phosphate = diphosphate + sn-3-O-(geranylgeranyl)glycerol 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64456">
        <property type="entry name" value="A64456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870763.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58647"/>
    <dbReference type="SMR" id="Q58647"/>
    <dbReference type="STRING" id="243232.MJ_1250"/>
    <dbReference type="DNASU" id="1452148"/>
    <dbReference type="EnsemblBacteria" id="AAB99253">
        <property type="protein sequence ID" value="AAB99253"/>
        <property type="gene ID" value="MJ_1250"/>
    </dbReference>
    <dbReference type="GeneID" id="1452148"/>
    <dbReference type="KEGG" id="mja:MJ_1250"/>
    <dbReference type="eggNOG" id="arCOG01085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068610_0_0_2"/>
    <dbReference type="InParanoid" id="Q58647"/>
    <dbReference type="OMA" id="ADAIFFM"/>
    <dbReference type="OrthoDB" id="94629at2157"/>
    <dbReference type="PhylomeDB" id="Q58647"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000138732" description="Geranylgeranylglyceryl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="172"/>
            <end position="178"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="203"/>
            <end position="204"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="225"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <sequence length="253" mass="27797" checksum="02EA882E3D718C0F" modified="1996-11-01" version="1">MKIKIGKVEKRLNQIIEEEGAVYLTLLDPEEENIEEIAENVKDYADAIMVGGSIGIVNLDETVKKIKKITKLPIILFPGNVDGLSRYADAVFYMSLMNSANTYWVVTAPTLGAITILKYNLEPIPMAYLCIEPAKKTAVGYVGEIREIPQNKPKITAMYCLSAKFFGMRWAYLEAGSGASYPVNNETIALSKKLSGINIIVGGGIRKPEIAYEKVLAGADAIVTGNLLEENPKAVEMMYDAIKKAGKEKLKNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q58660</accession>
    <name>PURL_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="1">FGAR amidotransferase II</shortName>
            <shortName evidence="1">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purL</name>
        <name type="ordered locus">MJ1264</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FGAMS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64457">
        <property type="entry name" value="G64457"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870777.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58660"/>
    <dbReference type="SMR" id="Q58660"/>
    <dbReference type="STRING" id="243232.MJ_1264"/>
    <dbReference type="PRIDE" id="Q58660"/>
    <dbReference type="EnsemblBacteria" id="AAB99270">
        <property type="protein sequence ID" value="AAB99270"/>
        <property type="gene ID" value="MJ_1264"/>
    </dbReference>
    <dbReference type="GeneID" id="1452162"/>
    <dbReference type="KEGG" id="mja:MJ_1264"/>
    <dbReference type="eggNOG" id="arCOG00641">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003100_0_1_2"/>
    <dbReference type="InParanoid" id="Q58660"/>
    <dbReference type="OMA" id="FIEPYQG"/>
    <dbReference type="OrthoDB" id="860at2157"/>
    <dbReference type="PhylomeDB" id="Q58660"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PTHR43555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100513" description="Phosphoribosylformylglycinamidine synthase subunit PurL">
        <location>
            <begin position="1"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="82"/>
            <end position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="301"/>
            <end position="303"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="519"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="520"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="522"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <sequence length="733" mass="80866" checksum="E0923221CB37722D" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q58692</accession>
    <name>BIOB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Biotin synthase</fullName>
            <ecNumber evidence="1">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioB</name>
        <name type="ordered locus">MJ1296</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64461">
        <property type="entry name" value="G64461"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58692"/>
    <dbReference type="SMR" id="Q58692"/>
    <dbReference type="STRING" id="243232.MJ_1296"/>
    <dbReference type="EnsemblBacteria" id="AAB99303">
        <property type="protein sequence ID" value="AAB99303"/>
        <property type="gene ID" value="MJ_1296"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1296"/>
    <dbReference type="eggNOG" id="arCOG00658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_2_1_2"/>
    <dbReference type="InParanoid" id="Q58692"/>
    <dbReference type="OMA" id="ADRFCMG"/>
    <dbReference type="PhylomeDB" id="Q58692"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000185564" description="Biotin synthase">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="47"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="358" mass="40722" checksum="7EB75DC2ED9996BF" modified="1996-11-01" version="1">MIFMEIETFLKKSLKNKIDFDDALYLYNNFSAIDLLYLAFKIKNRIKNNSKIKLCAIINAKSGKCKEDCIFCSQSIYSKCNIPIYPLKSKKEILEYAKKIIDECSKISSSIERGTLIGAESPNLMDVGYPNRGFPLWVERFSIVTSGKKINDDEFIEIVEAIELIKEETNLKVCCSLGLLDREKLKELKKLDVRIHNNLEASKNYFKNICSTHSYEDKVKVIKEAKKLDLEVCSGGIFGLGESVEERIKMAFELKELGVDSVPINILHPIEGTKAYEKIKNGEIKPISVSDALKLIALYKIIMPYAEIRLAGGRIYNLRDFQSYALMVLDGLMVGNYLTTKGRCLEDDLKMIADFHSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58702</accession>
    <name>Y1306_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1306</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1306</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64463">
        <property type="entry name" value="A64463"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58702"/>
    <dbReference type="STRING" id="243232.MJ_1306"/>
    <dbReference type="DNASU" id="1452208"/>
    <dbReference type="EnsemblBacteria" id="AAB99314">
        <property type="protein sequence ID" value="AAB99314"/>
        <property type="gene ID" value="MJ_1306"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1306"/>
    <dbReference type="eggNOG" id="arCOG06490">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_198706_0_0_2"/>
    <dbReference type="OMA" id="EAIPFSH"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107264" description="Uncharacterized protein MJ1306">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="83" mass="9337" checksum="0D413F375A61DEA4" modified="1996-11-01" version="1">MLDAILSNYLYYPSILAFLFGVLMGAKYRHKIGNIFGYLILTVVIAYFLKAFPYYDLLPLSCSYLSAVIGIIIGNRLFGGKMI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58722</accession>
    <name>Y1326_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized GTP-binding protein MJ1326</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1326</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64465">
        <property type="entry name" value="E64465"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58722"/>
    <dbReference type="SMR" id="Q58722"/>
    <dbReference type="STRING" id="243232.MJ_1326"/>
    <dbReference type="EnsemblBacteria" id="AAB99336">
        <property type="protein sequence ID" value="AAB99336"/>
        <property type="gene ID" value="MJ_1326"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1326"/>
    <dbReference type="eggNOG" id="arCOG00358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_2"/>
    <dbReference type="InParanoid" id="Q58722"/>
    <dbReference type="OMA" id="SAKHPGQ"/>
    <dbReference type="PhylomeDB" id="Q58722"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006074">
        <property type="entry name" value="GTP1-OBG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="PTHR43127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00905">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000205445" description="Uncharacterized GTP-binding protein MJ1326">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="1">
        <location>
            <begin position="85"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="2">
        <location>
            <begin position="314"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="91"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="137"/>
            <end position="141"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="267"/>
            <end position="270"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01228"/>
        </source>
    </evidence>
    <sequence length="391" mass="43649" checksum="1926F930A16FDD96" modified="1996-11-01" version="1">MLFLLSLKRIFIFRNHKFNIVGDVMGIEEEIRRIEEELKKTPYNKATQKHIGRLKAKLAKLREQAQSRGGGGGGKGYAVKKSGDATAAFVGFPSVGKSTLLNKLTNAKSEVGAYAFTTLTIVPGILEYKGAKIQLLDAPGIIVGASSGKGRGTEVLSAVRSADLILLTVDIYTLDHLPVLEKELYNVGIRLDQTPPDVKIKVKERGGINVSSTVPLTHIDEDTIEAILNEYRIHNADVVIREDITLEQFIDVVAGNRVYIPSLVVVNKIDLADEEYLKYIKQKLEEFGKDYILVSGNKGINLDLLKEKIYEKLGFIKIYLKPQGKKPDFDEPLIMRRGATVKDVCEKLHKDFVRNFRYAQVWGKSAKHPGQRVGLDHKLEDGDILTIVIKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-04" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58750</accession>
    <name>Y1355_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1355</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1355</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64469">
        <property type="entry name" value="B64469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870872.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58750"/>
    <dbReference type="STRING" id="243232.MJ_1355"/>
    <dbReference type="PRIDE" id="Q58750"/>
    <dbReference type="EnsemblBacteria" id="AAB99364">
        <property type="protein sequence ID" value="AAB99364"/>
        <property type="gene ID" value="MJ_1355"/>
    </dbReference>
    <dbReference type="GeneID" id="1452257"/>
    <dbReference type="KEGG" id="mja:MJ_1355"/>
    <dbReference type="eggNOG" id="arCOG05079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_450283_0_0_2"/>
    <dbReference type="InParanoid" id="Q58750"/>
    <dbReference type="OMA" id="VPIAEWV"/>
    <dbReference type="OrthoDB" id="6731at2157"/>
    <dbReference type="PhylomeDB" id="Q58750"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007548">
        <property type="entry name" value="DUF505"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04458">
        <property type="entry name" value="DUF505"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029056">
        <property type="entry name" value="DUF505"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107294" description="Uncharacterized protein MJ1355">
        <location>
            <begin position="1"/>
            <end position="571"/>
        </location>
    </feature>
    <sequence length="571" mass="65700" checksum="72DFE8C090E14C62" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58811</accession>
    <name>Y1416_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1416</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1416</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64476">
        <property type="entry name" value="G64476"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58811"/>
    <dbReference type="SMR" id="Q58811"/>
    <dbReference type="STRING" id="243232.MJ_1416"/>
    <dbReference type="DNASU" id="1452319"/>
    <dbReference type="EnsemblBacteria" id="AAB99426">
        <property type="protein sequence ID" value="AAB99426"/>
        <property type="gene ID" value="MJ_1416"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1416"/>
    <dbReference type="eggNOG" id="arCOG00637">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_648312_0_0_2"/>
    <dbReference type="InParanoid" id="Q58811"/>
    <dbReference type="OMA" id="IGGDMVI"/>
    <dbReference type="PhylomeDB" id="Q58811"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02691">
        <property type="entry name" value="PurM-like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009186">
        <property type="entry name" value="Ni_metllenz_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006346">
        <property type="entry name" value="Ni_metllenz_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000201463" description="Uncharacterized protein MJ1416">
        <location>
            <begin position="1"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="452" mass="50076" checksum="8B4C364CEDBABF92" modified="1996-11-01" version="1">MMDLEGYVRRCLRKKIPENKIIEDGFKRILEIKEDVDEEFAKKFIKAILEEVKTTEKFREIDDENLKTLLKYPKSGVTMGRMGVGSRGEGDFFVHREIARIVKSTKVKAYVSAEEQDDAGIVRADAKYIVAAIDGTHSRLSDFPFLGGFHVTRAALRDIYVMGAEAVALISDVHLADDGDVGKIFDFTAGICAVSEAVNVPLIGGSTLRVGGDMVIGDRLVSAVGAIGVIKEGEPTARRNAEVGDVILMTEGSGGGTITTTALYYGWFDVIYETLNVDFIKACQNLIRSGLIKKIHAMTDVTNGGLRGDAYEISKTAKVSLIFDKEKVYKTINPKVLEMLEVLNIDPLGVSTDSLMIICPEEYADDIKKVTGAIEVGYVEEGEESYLVDGNKKIPLKPMFRESAYTPVKKVVGERKPGDFEEMKEKVRRACDEAIKKKDFVVELLKERKKKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58845</accession>
    <name>Y1450_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1450</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64481">
        <property type="entry name" value="A64481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870970.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58845"/>
    <dbReference type="SMR" id="Q58845"/>
    <dbReference type="STRING" id="243232.MJ_1450"/>
    <dbReference type="DNASU" id="1452354"/>
    <dbReference type="EnsemblBacteria" id="AAB99458">
        <property type="protein sequence ID" value="AAB99458"/>
        <property type="gene ID" value="MJ_1450"/>
    </dbReference>
    <dbReference type="GeneID" id="1452354"/>
    <dbReference type="KEGG" id="mja:MJ_1450"/>
    <dbReference type="eggNOG" id="arCOG02232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052500_0_0_2"/>
    <dbReference type="InParanoid" id="Q58845"/>
    <dbReference type="OMA" id="CDAKWIP"/>
    <dbReference type="OrthoDB" id="43345at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107337" description="Uncharacterized protein MJ1450">
        <location>
            <begin position="1"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="371" mass="42027" checksum="8B76588950AF5992" modified="1996-11-01" version="1">MYDFAIIGSGVAGATLAKELRYRYKVAVIEKGKKPSYASEGKNVEINYVYGLGGSGVYSLGNAIKTEIKGYKIDKDIYKEIWEELKIKAPKDDFLNDIDKAFIELGFEKMEKFIDFDRCNKCGECARKICKAKWTPLNYLKESNANIITEFNIKAINYSNYYEILDDKGRKIKAKNLIISAGGINSPRILKKMIDDENIGKNLFIDTFVTVGGILEDSYLNKDISMLVYKKYKNFMLATHYSKLLINEIKKDYKDVKEKDIVGIMIKIKDENNGVVLDNDVKKEITKEDFKTLARGICKATKYLYKLGVDDIYTTIPRGSHPGGSLSLVVDEFEVREGLYVCDASLFKEALGVPPIVSIIALSKKFVREIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58850</accession>
    <name>Y1455_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1455</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1455</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64481">
        <property type="entry name" value="F64481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870975.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58850"/>
    <dbReference type="STRING" id="243232.MJ_1455"/>
    <dbReference type="DNASU" id="1452359"/>
    <dbReference type="EnsemblBacteria" id="AAB99469">
        <property type="protein sequence ID" value="AAB99469"/>
        <property type="gene ID" value="MJ_1455"/>
    </dbReference>
    <dbReference type="GeneID" id="1452359"/>
    <dbReference type="KEGG" id="mja:MJ_1455"/>
    <dbReference type="eggNOG" id="arCOG01894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_955177_0_0_2"/>
    <dbReference type="OMA" id="MILFEWG"/>
    <dbReference type="OrthoDB" id="45313at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107344" description="Uncharacterized protein MJ1455">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <sequence length="305" mass="35009" checksum="BA911EEC3B248C40" modified="1996-11-01" version="1">MILFEWGTYNALSTLKQAALLGTRITEIPPAVLSRRLPSGYYESYKKLGGEYFTSILAHGPYYSLSSEKGLKGHLSAIEKATLCGAEIYNYHLGKRVGDDLNYHLEVLKKFSEVNNEMIYSPEPATNIGEFGTLDELEELIKAAKEEDIKIIPSLQLENIFLNELGVYEKDDLDEAAEKADVDWWLKIFRRMDKISDYIMHFRFSQVIGLKYGKRFYKKRVPLGKGYPPVEPLTEALATYLVDNATRGGFKKVLFVYTGLPEVKYRDLIDLYAMIMKKSIDKLMSRESQVEYGDFYKVMSSEEEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58869</accession>
    <name>NOB1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Endoribonuclease Nob1</fullName>
            <shortName>RNase Nob1</shortName>
            <ecNumber evidence="3">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Endonuclease VapC5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Putative toxin VapC5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">nob1</name>
        <name type="synonym">vapC5</name>
        <name type="ordered locus">MJ1474</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature 3' end.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Has 2 structurally independent domains; the N-terminal PINc domain which binds Mn(2+), rRNA substrate and probably has endoribonuclease activity, and the C-terminal zinc ribbon domain which also binds rRNA substrate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64484">
        <property type="entry name" value="A64484"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58869"/>
    <dbReference type="SMR" id="Q58869"/>
    <dbReference type="STRING" id="243232.MJ_1474"/>
    <dbReference type="EnsemblBacteria" id="AAB99480">
        <property type="protein sequence ID" value="AAB99480"/>
        <property type="gene ID" value="MJ_1474"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1474"/>
    <dbReference type="eggNOG" id="arCOG00721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109674_0_0_2"/>
    <dbReference type="InParanoid" id="Q58869"/>
    <dbReference type="OMA" id="GCGREFD"/>
    <dbReference type="PhylomeDB" id="Q58869"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="PTHR12814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000156045" description="Endoribonuclease Nob1">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="PINc" evidence="3">
        <location>
            <begin position="35"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Flexible linker" evidence="1">
        <location>
            <begin position="147"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Zinc ribbon" evidence="1">
        <location>
            <begin position="153"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="197" mass="22781" checksum="70075BB626934F58" modified="1996-11-01" version="1">MLSLPPPEFYKLWIINKSPYQLFNIYRSGIMKVKVLDASAIIHGYNPIIEEGEHYTTPEVLEEIESKKIIVEQALDFGKLKIMSPNREYIKKVEEVVKKTGDNLSQQDIGVLALALNLNAILYTDDYGIQNVAKKLNIEVRGIAFEPTNKDFIWRKICEGCKKLYPVDYEEDICEICGSPLKRKMVKSRLKKKRKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58897</accession>
    <name>RNZ_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease Z</fullName>
            <shortName evidence="1">RNase Z</shortName>
            <ecNumber evidence="1">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnz</name>
        <name type="ordered locus">MJ1502</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="EMBO J." volume="21" first="2769" last="2777">
            <title>Assigning a function to a conserved group of proteins: the tRNA 3' - processing enzymes.</title>
            <authorList>
                <person name="Schiffer S."/>
                <person name="Roesch S."/>
                <person name="Marchfelder A."/>
            </authorList>
            <dbReference type="PubMed" id="12032089"/>
            <dbReference type="DOI" id="10.1093/emboj/21.11.2769"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99515" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99515.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64487">
        <property type="entry name" value="E64487"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58897"/>
    <dbReference type="SMR" id="Q58897"/>
    <dbReference type="STRING" id="243232.MJ_1502"/>
    <dbReference type="EnsemblBacteria" id="AAB99515">
        <property type="protein sequence ID" value="AAB99515"/>
        <property type="gene ID" value="MJ_1502"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1502"/>
    <dbReference type="eggNOG" id="arCOG00501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031317_2_1_2"/>
    <dbReference type="InParanoid" id="Q58897"/>
    <dbReference type="OMA" id="GTQRQMM"/>
    <dbReference type="PhylomeDB" id="Q58897"/>
    <dbReference type="BRENDA" id="3.1.26.11">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042779">
        <property type="term" value="P:tRNA 3'-trailer cleavage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000155924" description="Ribonuclease Z">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12032089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="317" mass="36034" checksum="9D8EE7E824063ED3" modified="2004-01-16" version="2">MIIMKLIFLGTGAAVPSKNRNHIGIAFKFGGEVFLFDCGENIQRQMLFTEVSPMKINHIFITHLHGDHILGIPGLLQSMGFFGREKELKIFGPEGTKEIIENSLKLGTHYIEFPIKVYEIYTKEPITIYKEENYEIIAYPTEHGIPSYAYIFKEIKKPRLDIEKAKKLGVKIGPDLKKLKNGEAVKNIYGEIIKPEYVLLPPKKGFCLAYSGDTLPLEDFGKYLKELGCDVLIHEATFDDSAKDAAKENMHSTIGDAVNIAKLANVKALILTHISARYDKEEYFNLYKMNVKQYNESFKIIISEDLKSYDIKKDLLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58922</accession>
    <name>Y1527_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0132 membrane protein MJ1527</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1527</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0132 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64490">
        <property type="entry name" value="F64490"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58922"/>
    <dbReference type="STRING" id="243232.MJ_1527"/>
    <dbReference type="EnsemblBacteria" id="AAB99545">
        <property type="protein sequence ID" value="AAB99545"/>
        <property type="gene ID" value="MJ_1527"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1527"/>
    <dbReference type="eggNOG" id="arCOG04344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095018_3_0_2"/>
    <dbReference type="InParanoid" id="Q58922"/>
    <dbReference type="OMA" id="FIRFHAM"/>
    <dbReference type="PhylomeDB" id="Q58922"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019109">
        <property type="entry name" value="DUF4870"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09685">
        <property type="entry name" value="DUF4870"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000158603" description="UPF0132 membrane protein MJ1527">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="140" mass="16481" checksum="1FD660864492BC72" modified="1996-11-01" version="1">MNIYLISKVFIKYHLFQNILKSYLLNFLVRLMALGLDRNMEGVLCYLLFWISGLIFLLLEREDDFIRFHAMQSFITFLSLNLIAIIVSAIPIIGWVASTLINIAIIILWIVGMIKAYNGERYKFPVFGDIAERYYREFLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58951</accession>
    <name>Y1556_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1556</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1556</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64494">
        <property type="entry name" value="C64494"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58951"/>
    <dbReference type="STRING" id="243232.MJ_1556"/>
    <dbReference type="PRIDE" id="Q58951"/>
    <dbReference type="DNASU" id="1452464"/>
    <dbReference type="EnsemblBacteria" id="AAB99576">
        <property type="protein sequence ID" value="AAB99576"/>
        <property type="gene ID" value="MJ_1556"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1556"/>
    <dbReference type="eggNOG" id="arCOG00360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048086_2_1_2"/>
    <dbReference type="InParanoid" id="Q58951"/>
    <dbReference type="OMA" id="RMANIML"/>
    <dbReference type="PhylomeDB" id="Q58951"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="PTHR38139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107410" description="Uncharacterized protein MJ1556">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="312" mass="35563" checksum="3185414AE35B24D1" modified="1998-12-15" version="2">MMDKIIQTLTLTFTFLYYSIPMLIVGLFISQILIESNIIKKIYFIGKIFTRLANLPEECGIAITTSFIEPRMANIMLVDFYKKGIINKKELYISSLIDAFPAMLRHWDSLLPILLATLGFFGIIYFIILVLIGFIQTLIFMAIGKITLKNREYKEDNTDKKIKLNKDVVYTAFKNTIKYGIPIIRDITIASIITSFLIEFGFFDYITEIIKNKAYYLPLSVEEITVAVTQPINYIGAFVLAGEFLNRGILDEIEVVRALLLGSILSSIPALRFLAPYYIGIYGFKDGFNLMMISTLVRILITALFVIITLIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58961</accession>
    <name>Y1566_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1566</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1566</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64495">
        <property type="entry name" value="E64495"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871090.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58961"/>
    <dbReference type="SMR" id="Q58961"/>
    <dbReference type="STRING" id="243232.MJ_1566"/>
    <dbReference type="EnsemblBacteria" id="AAB99592">
        <property type="protein sequence ID" value="AAB99592"/>
        <property type="gene ID" value="MJ_1566"/>
    </dbReference>
    <dbReference type="GeneID" id="1452474"/>
    <dbReference type="KEGG" id="mja:MJ_1566"/>
    <dbReference type="eggNOG" id="arCOG08283">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_611953_0_0_2"/>
    <dbReference type="InParanoid" id="Q58961"/>
    <dbReference type="OMA" id="TEWIPFG"/>
    <dbReference type="OrthoDB" id="22718at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107414" description="Uncharacterized protein MJ1566">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="447" mass="51527" checksum="1643E9D3633E4378" modified="1996-11-01" version="1">MGFKYLKIKNPKVILTEWIPFGKNYMTEFIDRITLKEYQRKRIKYFTASERRDIRYKAVFETSEYQTTVNIIEFIPETSVKFTAEIIGERKKDVFIYVDYLGRCIYSSEITKAGDEEEIVSLDNLSFVIPDLILDSSRIMSHLISPPQRYLLETLYGEIKVYKHVTVLTETVVNIDENTILEISQVIGAVKNIIEIDDGLIIFGDFGIFISHKNPEKFEKFIYYYPFIRSITGVSRDLFFKLNNIASKLEVISNTLASGVDLEDITEIRGELSRIDRELAVIEIVCGYLKEIVEFLNSSYPPNFGDFDLMILEKVEAERKLRRLIYRIAEIENILKSNDSLATSLTRLLTTISEDLERKIANQLAENTKYQVAIGEAMEVLEIGIFGVYALEAAHILLLTSGKDEILHHIKILGFPLEFWIILVVTILGVYVGKIVIEYRKKKVLGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58982</accession>
    <name>Y1587_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1587</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1587</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0417.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64498">
        <property type="entry name" value="B64498"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58982"/>
    <dbReference type="SMR" id="Q58982"/>
    <dbReference type="STRING" id="243232.MJ_1587"/>
    <dbReference type="EnsemblBacteria" id="AAB99616">
        <property type="protein sequence ID" value="AAB99616"/>
        <property type="gene ID" value="MJ_1587"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1587"/>
    <dbReference type="eggNOG" id="arCOG00306">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072983_0_0_2"/>
    <dbReference type="InParanoid" id="Q58982"/>
    <dbReference type="OMA" id="CITDHND"/>
    <dbReference type="PhylomeDB" id="Q58982"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' exodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107428" description="Uncharacterized protein MJ1587">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="171" mass="19198" checksum="7D4F7B936C5C7472" modified="1996-11-01" version="1">MKADLHIHTKYSGIGKFWKLKFPDSVEEPRNILKVAKKKGIEVVAITDHNTIRGGVETKKLEKEFGVEVVIGSEIMTTEGEIIGLFLNEDIPKGLSPEETIEKIKEQGGLAIAPHPYSPICKALGDRIFDLDLDGVEVFNAYHRDGIVNNIALNKVIKELPQKAFCIYWRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q59009</accession>
    <name>Y1614_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1614</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1614</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1311.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64501">
        <property type="entry name" value="E64501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871139.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59009"/>
    <dbReference type="SMR" id="Q59009"/>
    <dbReference type="STRING" id="243232.MJ_1614"/>
    <dbReference type="EnsemblBacteria" id="AAB99634">
        <property type="protein sequence ID" value="AAB99634"/>
        <property type="gene ID" value="MJ_1614"/>
    </dbReference>
    <dbReference type="GeneID" id="1452523"/>
    <dbReference type="KEGG" id="mja:MJ_1614"/>
    <dbReference type="eggNOG" id="arCOG01895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_7_2_2"/>
    <dbReference type="InParanoid" id="Q59009"/>
    <dbReference type="OMA" id="PTIDLNP"/>
    <dbReference type="OrthoDB" id="46548at2157"/>
    <dbReference type="PhylomeDB" id="Q59009"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107437" description="Uncharacterized protein MJ1614">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="251" mass="28930" checksum="7C1AFDABF506E26A" modified="1998-06-01" version="2">MKLGVSTSLFLDTDKNLSDALEILEERVKYVELGCDGNLNVMSDGNIELAQSYDLKYTLHCPITDLNLSSYRERIRKVSLDFVRDVLEVAIKVDAKLIVLHPGYCVFKYDYEKALNSLIKSLNDLNNIQEEFGVQITIENMPSYDMFMFRNPDKEIIENLGELKITLDIGHSFLNKNIENFLKISDKIAHIHIHDNNGEFDEHLCIGKGKINFNNFKKDLKKINAIKMIELQNKSIDDLDLCIDNLKEILR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="140" xmlns="http://uniprot.org/uniprot">
    <accession>Q59011</accession>
    <name>IMDH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="1">IMP dehydrogenase</shortName>
            <shortName evidence="1">IMPD</shortName>
            <shortName evidence="1">IMPDH</shortName>
            <ecNumber evidence="1">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaB</name>
        <name type="ordered locus">MJ1616</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + IMP + NAD(+) = H(+) + NADH + XMP</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="G64501">
        <property type="entry name" value="G64501"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="STRING" id="243232.MJ_1616"/>
    <dbReference type="PRIDE" id="Q59011"/>
    <dbReference type="EnsemblBacteria" id="AAB99638">
        <property type="protein sequence ID" value="AAB99638"/>
        <property type="gene ID" value="MJ_1616"/>
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    <dbReference type="GeneID" id="1452525"/>
    <dbReference type="KEGG" id="mja:MJ_1616"/>
    <dbReference type="eggNOG" id="arCOG00612">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022552_2_1_2"/>
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    <dbReference type="OMA" id="MGYCGAK"/>
    <dbReference type="OrthoDB" id="58700at2157"/>
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        <property type="reaction ID" value="UER00295"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_01964">
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
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    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
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    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="PTHR11911"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
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    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000093719" description="Inosine-5'-monophosphate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="96"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="156"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="1">
        <location>
            <position position="306"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="405"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="299"/>
            <end position="301"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="339"/>
            <end position="341"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="362"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="386"/>
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        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="423"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="478"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <sequence length="496" mass="53317" checksum="C3E03FDDF3898396" modified="1997-11-01" version="1">MFLKKLIEAKKAYTFDDVLLVPNASWVEPKDTDVSTDLAGLKLNIPIVSAAMDTVTEKEMAIALARLGGLGVIHRNMSIEEQVHQVQAVKKADEVVIKDVITVSPDDTVGEAINVMETYSISGLPVVDNEDKLVGIITHRDVKAIEDKTKKVKDVMTKDVVCAKEDVEEEEALELMYANRVERLPIVDDENRLIGIITLRDILKRRKYPQAARDKKGRLLVAAACGPHDFERAKALIEAEVDAIAIDCAHAHNMRVVENVKKFKEMLEGTDIKLIVGNIATKEAAEDLIKAGADVLKVGIGPGSICTTRVVAGVGVPQLTAVAEVADVAKEHNVPIIADGGIRYSGDIAKAIAAGADAVMLGSLLAGTDEAPGQLMVINGRKYKQYRGMGSLGAMTGGVGAGADRYFQAPAKSHMKHVKLVPEGVEGAVPYKGPVSEVVFQLIGGLRASMGYCGAKNLKEMQEKARFVIITPSGQVESHPHDIIITNEAPNYPLGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q59014</accession>
    <name>BPL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative biotin ligase</fullName>
            <ecNumber>6.3.4.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1619</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) + N(6)-biotinyl-L-lysyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:11756"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10505"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:83144"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.15"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the biotin--protein ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.15"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64502">
        <property type="entry name" value="B64502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871144.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2EJ9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-237"/>
    </dbReference>
    <dbReference type="PDBsum" id="2EJ9"/>
    <dbReference type="AlphaFoldDB" id="Q59014"/>
    <dbReference type="SMR" id="Q59014"/>
    <dbReference type="STRING" id="243232.MJ_1619"/>
    <dbReference type="EnsemblBacteria" id="AAB99640">
        <property type="protein sequence ID" value="AAB99640"/>
        <property type="gene ID" value="MJ_1619"/>
    </dbReference>
    <dbReference type="GeneID" id="1452528"/>
    <dbReference type="KEGG" id="mja:MJ_1619"/>
    <dbReference type="eggNOG" id="arCOG01940">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051096_3_1_2"/>
    <dbReference type="InParanoid" id="Q59014"/>
    <dbReference type="OMA" id="AVWKHIE"/>
    <dbReference type="OrthoDB" id="42041at2157"/>
    <dbReference type="PhylomeDB" id="Q59014"/>
    <dbReference type="EvolutionaryTrace" id="Q59014"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin-[acetyl-CoA-carboxylase] ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0092">Biotin</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000064982" description="Putative biotin ligase">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="BPL/LPL catalytic" evidence="1">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="26"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="51"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="65"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="81"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="95"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="116"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="133"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="142"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="188"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="194"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="204"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="214"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="224"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="230"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="233"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2EJ9"/>
        </source>
    </evidence>
    <sequence length="237" mass="27185" checksum="602506218437B1F6" modified="1996-11-01" version="1">MEIIHLSEIDSTNDYAKELAKEGKRNFIVLADKQNNGKGRWGRVWYSDEGGLYFSMVLDSKLYNPKVINLLVPICIIEVLKNYVDKELGLKFPNDIMVKVNDNYKKLGGILTELTDDYMIIGIGINVNNQIRNEIREIAISLKEITGKELDKVEILSNFLKTFESYLEKLKNKEIDDYEILKKYKKYSITIGKQVKILLSNNEIITGKVYDIDFDGIVLGTEKGIERIPSGICIHVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q59018</accession>
    <name>Y1624_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0292 protein MJ1624</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1624</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds two Mg(2+) per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0292 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99642" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99642.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64502">
        <property type="entry name" value="F64502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496879.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59018"/>
    <dbReference type="SMR" id="Q59018"/>
    <dbReference type="STRING" id="243232.MJ_1624"/>
    <dbReference type="EnsemblBacteria" id="AAB99642">
        <property type="protein sequence ID" value="AAB99642"/>
        <property type="gene ID" value="MJ_1624"/>
    </dbReference>
    <dbReference type="GeneID" id="1452533"/>
    <dbReference type="KEGG" id="mja:MJ_1624"/>
    <dbReference type="eggNOG" id="arCOG01486">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140789_0_0_2"/>
    <dbReference type="InParanoid" id="Q59018"/>
    <dbReference type="OMA" id="VMILTDF"/>
    <dbReference type="OrthoDB" id="99443at2157"/>
    <dbReference type="PhylomeDB" id="Q59018"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01027">
        <property type="entry name" value="TOPRIM_RNase_M5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01095">
        <property type="entry name" value="UPF0292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034141">
        <property type="entry name" value="TOPRIM_RNase_M5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022972">
        <property type="entry name" value="UPF0292"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13662">
        <property type="entry name" value="Toprim_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000143951" description="UPF0292 protein MJ1624">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="23"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="128" mass="15196" checksum="157D50F40100229F" modified="2003-11-28" version="2">MRRDEYFEKLLEVIEELKIEAEEKPIIVEGKRDVESLEKLGVEGTFIIIAKTPIYLIADELVRKRVKEVILLTDFDRRGRMLAKAIIEEFRHRGIKVNTKIRHEIFIYTNSGIRDIESLFSYVNKRLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q59035</accession>
    <name>Y1641_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1641</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1641</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64504">
        <property type="entry name" value="G64504"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59035"/>
    <dbReference type="SMR" id="Q59035"/>
    <dbReference type="STRING" id="243232.MJ_1641"/>
    <dbReference type="EnsemblBacteria" id="AAB99661">
        <property type="protein sequence ID" value="AAB99661"/>
        <property type="gene ID" value="MJ_1641"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1641"/>
    <dbReference type="eggNOG" id="arCOG00318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_989064_0_0_2"/>
    <dbReference type="OMA" id="DRCVNIV"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107454" description="Uncharacterized protein MJ1641">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <sequence length="287" mass="33433" checksum="2DAE68A6F06B7642" modified="1996-11-01" version="1">MNLWDNMLRGKEATLAGIIRVIIEEEPETQDEIAEKLGISRRYVAKLLKPLIDEKIVRHPYIVDMSKLHKINLEFDEYILMKEIKTTLEKMEKTLLNNLDLVYTALKNSDKKLAEDIIIKDYALNKMEEEVRILLSMNALKYLPGAYANALATIASNLERLGDYIANIAEEVVHGLKLDKDIENEVNMIFTLLKEMLTEAIDVVKSKKKETKIHELEEKLHKNLELLLNKVLENKREDLNFYVQFGMFLKDIERFGDRCVNIVDIALELYHNIPRNPIPERLKRGML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="57" xmlns="http://uniprot.org/uniprot">
    <accession>Q60267</accession>
    <name>Y3502_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL02</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL02</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64510">
        <property type="entry name" value="B64510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890049.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60267"/>
    <dbReference type="SMR" id="Q60267"/>
    <dbReference type="STRING" id="243232.MJ_ECL02"/>
    <dbReference type="EnsemblBacteria" id="AAC37076">
        <property type="protein sequence ID" value="AAC37076"/>
        <property type="gene ID" value="MJ_ECL02"/>
    </dbReference>
    <dbReference type="GeneID" id="1450789"/>
    <dbReference type="KEGG" id="mja:MJ_ECL02"/>
    <dbReference type="HOGENOM" id="CLU_1943864_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107494" description="Uncharacterized protein MJECL02">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <sequence length="129" mass="15215" checksum="F6C24D21B1495B77" modified="1997-11-01" version="1">MLMMSIILSMCIQMIRIKSGLSSVICVYSQMNNFRIFWWDIMTAFEELLELPTIEAIYKLKKLILKMESSKNSEVYYYQGVIKRIIQRLSLLDQEKSLKENLKFCVDNYILSQRTIEDLKTAIEIASKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q60274</accession>
    <name>Y3512_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL12</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64511">
        <property type="entry name" value="D64511"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890059.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60274"/>
    <dbReference type="SMR" id="Q60274"/>
    <dbReference type="STRING" id="243232.MJ_ECL12"/>
    <dbReference type="PRIDE" id="Q60274"/>
    <dbReference type="EnsemblBacteria" id="AAC37085">
        <property type="protein sequence ID" value="AAC37085"/>
        <property type="gene ID" value="MJ_ECL12"/>
    </dbReference>
    <dbReference type="GeneID" id="1450798"/>
    <dbReference type="KEGG" id="mja:MJ_ECL12"/>
    <dbReference type="eggNOG" id="arCOG05094">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243068_0_0_2"/>
    <dbReference type="InParanoid" id="Q60274"/>
    <dbReference type="OMA" id="HANGKDN"/>
    <dbReference type="OrthoDB" id="82593at2157"/>
    <dbReference type="PhylomeDB" id="Q60274"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024508">
        <property type="entry name" value="DUF3226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11536">
        <property type="entry name" value="DUF3226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107504" description="Uncharacterized protein MJECL12">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <sequence length="241" mass="27268" checksum="C5D3C7742A35A097" modified="1997-11-01" version="1">MRILLLEGITDVAFFIPILKKLYGFSEISCDGIIRAEKMGDISKPICLENEDVKLIVFHSGGKSKQKHALTAMLTAIKMGYLSNIKILGIARDIDQEHDVKNWTKSIIKNAGFEVKEGDKFLIIEDLNLKIAVLGIANYDEDDFNIPSFELKRELEAVITDMAKEISIIEKFKNSLESLSNDAERRLKPKDITHVLAIAKNFDGDSMSGLYRKFIEEQINNKNKVNFLLTLICILPCLTIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q60280</accession>
    <name>Y3520_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL20</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0215.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64512">
        <property type="entry name" value="C64512"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890067.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60280"/>
    <dbReference type="SMR" id="Q60280"/>
    <dbReference type="STRING" id="243232.MJ_ECL20"/>
    <dbReference type="EnsemblBacteria" id="AAC37091">
        <property type="protein sequence ID" value="AAC37091"/>
        <property type="gene ID" value="MJ_ECL20"/>
    </dbReference>
    <dbReference type="GeneID" id="1450804"/>
    <dbReference type="KEGG" id="mja:MJ_ECL20"/>
    <dbReference type="eggNOG" id="arCOG03413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1700308_0_0_2"/>
    <dbReference type="InParanoid" id="Q60280"/>
    <dbReference type="OrthoDB" id="75163at2157"/>
    <dbReference type="PhylomeDB" id="Q60280"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="SSF52799"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107508" description="Uncharacterized protein MJECL20">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="147" mass="16976" checksum="9F615C1406747570" modified="1997-11-01" version="1">MGKCRHNGEVSIFGVRPASFPYFPFNLMDRIGGFVILDELWLRRWCEIIEYPMKIPTLYVPIEDYGIPTVEDMDLIVDFIKYHVSNGREVVVSCIGGHGRTGTVLAIWAGLNGVENPIEYVRECYCECAVETEEQEEFVMEYLKKRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q60281</accession>
    <name>Y3521_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL21</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64512">
        <property type="entry name" value="D64512"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890068.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60281"/>
    <dbReference type="PRIDE" id="Q60281"/>
    <dbReference type="EnsemblBacteria" id="AAC37092">
        <property type="protein sequence ID" value="AAC37092"/>
        <property type="gene ID" value="MJ_ECL21"/>
    </dbReference>
    <dbReference type="GeneID" id="1450805"/>
    <dbReference type="KEGG" id="mja:MJ_ECL21"/>
    <dbReference type="HOGENOM" id="CLU_634033_0_0_2"/>
    <dbReference type="OrthoDB" id="144347at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107509" description="Uncharacterized protein MJECL21">
        <location>
            <begin position="1"/>
            <end position="432"/>
        </location>
    </feature>
    <sequence length="432" mass="51082" checksum="DBADF2C5C43A4F90" modified="1997-11-01" version="1">MWRGVGMVVQQFINRIRNCPLARDIIKKFFIPVIKERKNIEKEVEDILKVLRKNYCSGEYANKDCICAWFKFFEDVGRLHEALELKAFWEIIFPNHLNDLSESLQLFISNYAYMRPFGSSYSSIRVRIHGFIGAVCIDRNFNLHNNTWISNICNCYENNLRMLGLGFYTSDTLDPRYNINPSCNINSTTTNKCGLLYIISNTQITASLVSYLLNLLRNGNIYDAYNFLIRIRGVGDKIACLFLRDISIIHNLMLYGDCRDLLYKPIDRVVKKVIEELVKCKCKLNYNFDINEVLEKFDYTECRCIQKLRKNAKIDSNDFKTLYPYKIFLYEYSKKCKLDQRYVEMGMWFFNSKVAKYYNRRLCKYTIQEACKDLIKRLRKAINNTNIRYIGNNQFEININGKIIIVSEDELIDLILKGEIEIYDELLDIIFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q60293</accession>
    <name>Y3538_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL38</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL38</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64514">
        <property type="entry name" value="E64514"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890085.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60293"/>
    <dbReference type="DNASU" id="1450820"/>
    <dbReference type="EnsemblBacteria" id="AAC37107">
        <property type="protein sequence ID" value="AAC37107"/>
        <property type="gene ID" value="MJ_ECL38"/>
    </dbReference>
    <dbReference type="GeneID" id="1450820"/>
    <dbReference type="KEGG" id="mja:MJ_ECL38"/>
    <dbReference type="eggNOG" id="arCOG02121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1072047_0_0_2"/>
    <dbReference type="InParanoid" id="Q60293"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10130">
        <property type="entry name" value="PIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107521" description="Uncharacterized protein MJECL38">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="259" mass="30076" checksum="00AEC07D21973C0A" modified="1997-11-01" version="1">MANMQSLTNIEVQRFHDCEWEYFKEFDDEFNKLWNEIEKTLGRDFINYLSAYFQKNLVYMLGKEFKLKLVVDTNIIFSQVLSYVTKGELPWILDFINNPFIELYAPQLIVDELKNKIENVLPKKCKKKNIDENKAKSKAIKIANIILSNIKIINDKKSNNWSKIAYNLIGHRDVKDIPFVTLALSLDTHGIITRDKDFKDQKIIKIWKVGEVKVVLTELSQGSFSFCIMNVTAPLAFKICTSIIITILEIVTSIIKKTN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q60302</accession>
    <name>Y3403_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS03</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS03</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64516">
        <property type="entry name" value="C64516"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890096.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60302"/>
    <dbReference type="SMR" id="Q60302"/>
    <dbReference type="STRING" id="243232.MJ_ECS03"/>
    <dbReference type="EnsemblBacteria" id="AAC37061">
        <property type="protein sequence ID" value="AAC37061"/>
        <property type="gene ID" value="MJ_ECS03"/>
    </dbReference>
    <dbReference type="GeneID" id="1450830"/>
    <dbReference type="KEGG" id="mja:MJ_ECS03"/>
    <dbReference type="eggNOG" id="arCOG04492">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151711_0_0_2"/>
    <dbReference type="InParanoid" id="Q60302"/>
    <dbReference type="OMA" id="IFYASRE"/>
    <dbReference type="OrthoDB" id="103001at2157"/>
    <dbReference type="PhylomeDB" id="Q60302"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007176">
        <property type="entry name" value="DUF365"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04033">
        <property type="entry name" value="DUF365"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006031">
        <property type="entry name" value="UCP006031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107484" description="Uncharacterized protein MJECS03">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <sequence length="108" mass="13210" checksum="478DFC06482D0CDE" modified="1997-11-01" version="1">MKIIFYASREEQGFYGEAEIEEVEFFENPMKILEKYKNNLFLTEEEFKKYIEDSNKKWGYGKKKKKPWIVIILKNIRKYPKVVKPKRFIPVCGKYVKEDEYEQILKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="54" xmlns="http://uniprot.org/uniprot">
    <accession>Q60303</accession>
    <name>Y3404_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS04</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS04</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64516">
        <property type="entry name" value="D64516"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890097.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60303"/>
    <dbReference type="SMR" id="Q60303"/>
    <dbReference type="EnsemblBacteria" id="AAC37062">
        <property type="protein sequence ID" value="AAC37062"/>
        <property type="gene ID" value="MJ_ECS04"/>
    </dbReference>
    <dbReference type="GeneID" id="1450831"/>
    <dbReference type="KEGG" id="mja:MJ_ECS04"/>
    <dbReference type="HOGENOM" id="CLU_1881088_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038400">
        <property type="entry name" value="NSP3_SUD-M_sf_bCoV"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107485" description="Uncharacterized protein MJECS04">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <sequence length="135" mass="15786" checksum="3C4A9DFB71D51AC0" modified="1997-11-01" version="1">MLKKAIIKLLGIDNYIEKIEELEGERKEIFENFEKLNKKLDNFENELNFIKKEIERIQLEFNNVINSNNNDYVSKKELNDIVNQLNTLSTLVNGLIINISHGNNLKESSNTVDDVKNRLLELLQSEKDYCITEIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q60322</accession>
    <name>Y016_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized IS-like element protein MJ0016/MJ1621.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0016</name>
    </gene>
    <gene>
        <name type="ordered locus">MJ1621.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64301">
        <property type="entry name" value="H64301"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60322"/>
    <dbReference type="EnsemblBacteria" id="AAB98001">
        <property type="protein sequence ID" value="AAB98001"/>
        <property type="gene ID" value="MJ_0016"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99645">
        <property type="protein sequence ID" value="AAB99645"/>
        <property type="gene ID" value="MJ_1621.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0016"/>
    <dbReference type="KEGG" id="mja:MJ_1621.1"/>
    <dbReference type="HOGENOM" id="CLU_2968473_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106656" description="Uncharacterized IS-like element protein MJ0016/MJ1621.1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <sequence length="58" mass="6622" checksum="1667E9FB91E42B63" modified="1997-11-01" version="1">MLFIDAMPIKTKELVRKTRHERIGISKLIKKTVVLVTIHRKNVGILDIKQLSLPMGSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q60380</accession>
    <name>Y074_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0074</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0074</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64309">
        <property type="entry name" value="B64309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869566.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60380"/>
    <dbReference type="SMR" id="Q60380"/>
    <dbReference type="STRING" id="243232.MJ_0074"/>
    <dbReference type="EnsemblBacteria" id="AAB98056">
        <property type="protein sequence ID" value="AAB98056"/>
        <property type="gene ID" value="MJ_0074"/>
    </dbReference>
    <dbReference type="GeneID" id="1450913"/>
    <dbReference type="KEGG" id="mja:MJ_0074"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q60380"/>
    <dbReference type="OMA" id="YVIEECK"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="PhylomeDB" id="Q60380"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184665" description="Uncharacterized ATP-binding protein MJ0074">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="358" mass="42310" checksum="34BBAEF2DFCF4876" modified="1997-11-01" version="1">MKFFDREKEIAEILHILNREPDDVYFIYGPINSGKTALINEIINNRLDKDKYVVFYFDLREIFISKYDDFIEVLFEEYEGNKKPVEIIKSLIKDVPSLCGIPAPKNTLEEILKKKTTKNVFRYITKVLMDIKKEGKQPILIIDELQKIGDMKINGFLIYELFNYFVSLTKHKHLCHVFCLSSDSLFIERVYNEAMLDGRAKYLLVDDFDKETALKFMDFLAKENNISLTNEDKELIYNYVGGKPKDIKYVVEESNFKDLKEVLDYLLNDEISKLDMFLEILDYSKPRVEVGNEVIEINKEDIIKALRLFKDKYEIPKKDIPTPVYVYLVKENILFLNPQKRILKPQSYLVWNAIKRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q60382</accession>
    <name>ARGB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetylglutamate kinase</fullName>
            <ecNumber evidence="1">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">NAG kinase</fullName>
            <shortName evidence="1">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argB</name>
        <name type="ordered locus">MJ0069</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamyl 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64308">
        <property type="entry name" value="E64308"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60382"/>
    <dbReference type="SMR" id="Q60382"/>
    <dbReference type="STRING" id="243232.MJ_0069"/>
    <dbReference type="EnsemblBacteria" id="AAB98048">
        <property type="protein sequence ID" value="AAB98048"/>
        <property type="gene ID" value="MJ_0069"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0069"/>
    <dbReference type="eggNOG" id="arCOG00862">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053680_0_0_2"/>
    <dbReference type="InParanoid" id="Q60382"/>
    <dbReference type="OMA" id="EGLYEDW"/>
    <dbReference type="PhylomeDB" id="Q60382"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="SSF53633"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000112694" description="Acetylglutamate kinase">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="68"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <sequence length="300" mass="32725" checksum="B54C58AAFA43F7DA" modified="1997-11-01" version="1">MGMDMIEMIEKAEILMEALPFIQKFYGKIFVIKYGGHAMIDEKAKNWTAQDVVLLKYVGINPVVVHGGGPEINKAMEKMGKKPEFVHGLRVTDEETLDIVEMVLAGKINGDIVSKLSKFGGKAVGLSGKSGRIILAKKKLKKIKTEKGEEIEVDLGRVGETVEVNTELLEILINNGYIPVVSPIGLDEKGEAYNLNADTVAGDIAGALKAEKLILITDVDGIMDDINNPETLHRKLTASELKEMIEDGRIKGGMIPKAESALYALEHGVKSVHIINGKIPHALLLEIFTEEGIGTMITRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q60384</accession>
    <name>Y077_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0077</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0077</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64309">
        <property type="entry name" value="D64309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869568.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60384"/>
    <dbReference type="SMR" id="Q60384"/>
    <dbReference type="STRING" id="243232.MJ_0077"/>
    <dbReference type="PRIDE" id="Q60384"/>
    <dbReference type="EnsemblBacteria" id="AAB98058">
        <property type="protein sequence ID" value="AAB98058"/>
        <property type="gene ID" value="MJ_0077"/>
    </dbReference>
    <dbReference type="GeneID" id="1450915"/>
    <dbReference type="KEGG" id="mja:MJ_0077"/>
    <dbReference type="eggNOG" id="arCOG00442">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036888_0_0_2"/>
    <dbReference type="InParanoid" id="Q60384"/>
    <dbReference type="OMA" id="FYLFFKY"/>
    <dbReference type="OrthoDB" id="13269at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032781">
        <property type="term" value="P:positive regulation of ATP-dependent activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106683" description="Uncharacterized protein MJ0077">
        <location>
            <begin position="1"/>
            <end position="382"/>
        </location>
    </feature>
    <sequence length="382" mass="44917" checksum="EC9C2F7C4EBEA695" modified="1997-11-01" version="1">MIRYDKYDKMVWEGCKNKITFHLSERETEIVFYLFFKYEVEILTETDLIKKIVRDRRFKNVKSITTLDENYSLIATEFFCEKLKELKEKGREEDISELLDELESYMENITSSFSSFGSGEGYKSYTDPKKKLELTEKLLKNNKLKEFMKVLGKFKRMAIKKYKTKIKHFSGEKYSINLGNNLINLLSSEYKNFAEEILFVDLLRRYNENKPLNYKILENNENCGDFVVCLDLSGSMRGNKEIWAKAIALCLMDISLKRNKRYISILFDDGVRDIKIYEKKVSFDEILEFASVFYGGGTNFEKPLREALKFNGDIVFITDGECEVSLEFLEKIKEEKQRRKIKIYSICINTKPTVSLRQISDVSVTIYELTSKTAEKVFDMLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q60387</accession>
    <name>MCRZ_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase II subunit gamma</fullName>
            <shortName>MCR II gamma</shortName>
            <ecNumber evidence="1">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme-B sulfoethylthiotransferase gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mrtG</name>
        <name type="synonym">mtrG</name>
        <name type="ordered locus">MJ0082</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="1">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-coenzyme M reductase gamma subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64310">
        <property type="entry name" value="B64310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869574.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60387"/>
    <dbReference type="SMR" id="Q60387"/>
    <dbReference type="STRING" id="243232.MJ_0082"/>
    <dbReference type="PRIDE" id="Q60387"/>
    <dbReference type="EnsemblBacteria" id="AAB98062">
        <property type="protein sequence ID" value="AAB98062"/>
        <property type="gene ID" value="MJ_0082"/>
    </dbReference>
    <dbReference type="GeneID" id="1450921"/>
    <dbReference type="KEGG" id="mja:MJ_0082"/>
    <dbReference type="eggNOG" id="arCOG04858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1092436_0_0_2"/>
    <dbReference type="InParanoid" id="Q60387"/>
    <dbReference type="OMA" id="GHRNPGE"/>
    <dbReference type="OrthoDB" id="51032at2157"/>
    <dbReference type="PhylomeDB" id="Q60387"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00539">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003178">
        <property type="entry name" value="Me_CoM_Rdtase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036994">
        <property type="entry name" value="Me_CoM_Rdtase_gsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02240">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000264">
        <property type="entry name" value="Meth_CoM_rd_gama"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03259">
        <property type="entry name" value="met_CoM_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147477" description="Methyl-coenzyme M reductase II subunit gamma">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P11562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="266" mass="30764" checksum="51E7B3C7F984157A" modified="1997-11-01" version="1">MAYKPQFYPGNTLIAENRRKHMNPEVELKKLRDIPDDEIVKILGHRNPGESYKTVHPPLEEMDFEEDPIKDIVEPIQGAKEGVRVRYIQFADSMYNAPAQPYDRARTYMWRFRGIDTGTLSGRQVIEMRELDLEKISKNFLIDTEFFDPATCGIRGATVHGHSLRLDENGLMFDGLQRYIYDEKTGHVLYVKDQVGRPLDEPVDVGEPLPHDYLAKITTIYRKDNIGMREDKEALEVVQIIHEARTKGGFGLEVFKKDLKKRLGEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q60388</accession>
    <name>Y075_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0075</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0075</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64309">
        <property type="entry name" value="C64309"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60388"/>
    <dbReference type="SMR" id="Q60388"/>
    <dbReference type="STRING" id="243232.MJ_0075"/>
    <dbReference type="DNASU" id="1450914"/>
    <dbReference type="EnsemblBacteria" id="AAB98065">
        <property type="protein sequence ID" value="AAB98065"/>
        <property type="gene ID" value="MJ_0075"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0075"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q60388"/>
    <dbReference type="PhylomeDB" id="Q60388"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184666" description="Uncharacterized ATP-binding protein MJ0075">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="365" mass="43328" checksum="DDE2574318A3E099" modified="1997-11-01" version="1">MIMKFFDREREINEILGILDETPDNIYFIYGPINSGKTTLMMEIINRLKDDKKYRIFYYNLRGVRISSYSDFFDIMFEIREDNKFKQMVKDADVLVEGIKFIEKTAKLFNESIILPSDLAKVILSKQKGFDVFRYLERVFREMNKKGLKPVIIIDELQRLKGLKSNGELIDDLFNFFVRLTKELHITHCFCLSSDSLFIEYVYDRAELRGRADYILVDDFDKETALKFMDFLSEDILGRKLSEDEKELIYSYVGGKPKDVYDVIIKLKLGKELKDILEFMLKEEIQKLKYFLEDVKEDDEELYNKIVDALKIFKENYEIEDIKIPKNIREFLVKKNILFLNPIEGTLKPQSFLVWNAIKKLLNGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>P54039</accession>
    <name>RS4E_METJA</name>
    <protein>
        <recommendedName>
            <fullName>30S ribosomal protein S4e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rps4e</name>
        <name type="ordered locus">MJ0468</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64358">
        <property type="entry name" value="D64358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869968.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54039"/>
    <dbReference type="SMR" id="P54039"/>
    <dbReference type="STRING" id="243232.MJ_0468"/>
    <dbReference type="EnsemblBacteria" id="AAB98457">
        <property type="protein sequence ID" value="AAB98457"/>
        <property type="gene ID" value="MJ_0468"/>
    </dbReference>
    <dbReference type="GeneID" id="1451330"/>
    <dbReference type="KEGG" id="mja:MJ_0468"/>
    <dbReference type="eggNOG" id="arCOG04093">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060400_0_0_2"/>
    <dbReference type="InParanoid" id="P54039"/>
    <dbReference type="OMA" id="FPVGLMD"/>
    <dbReference type="OrthoDB" id="89943at2157"/>
    <dbReference type="PhylomeDB" id="P54039"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="KOW_RPS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_S4e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_S4e_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_S4e_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_S4e_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_S4e_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="PTHR11581"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130849" description="30S ribosomal protein S4e">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding">
        <location>
            <begin position="43"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="244" mass="27659" checksum="6759485019B86AC7" modified="1996-10-01" version="1">MAKKGPKRHLKRLAAPVRWELPRKIHKFTVRPLPGAHPMSESLPLLLIVRDILKYADNAREAKKIIKMGKVLVDGRVRKEEKLPVGLMDVVSLPDANENYRVLFDRKGRIKLKPTENPDVKLCKIKNKTVIKGGHIQLNLHDGRNIVIKVSDPTKAEEDVYKTGDTLLISIPEQEIKAHIPFEVGKLAYITGGKHVGDFAKIVEIERRGIYPDIVTLENMDGEKFKTVKDYVFVVGDEEPIIKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>P54048</accession>
    <name>RL12_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl12</name>
        <name type="ordered locus">MJ0508</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64363">
        <property type="entry name" value="D64363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870009.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54048"/>
    <dbReference type="SMR" id="P54048"/>
    <dbReference type="STRING" id="243232.MJ_0508"/>
    <dbReference type="EnsemblBacteria" id="AAB98498">
        <property type="protein sequence ID" value="AAB98498"/>
        <property type="gene ID" value="MJ_0508"/>
    </dbReference>
    <dbReference type="GeneID" id="1451370"/>
    <dbReference type="KEGG" id="mja:MJ_0508"/>
    <dbReference type="eggNOG" id="arCOG04287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114656_2_0_2"/>
    <dbReference type="InParanoid" id="P54048"/>
    <dbReference type="OMA" id="EYIYAAM"/>
    <dbReference type="OrthoDB" id="128352at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_L12/P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_L12_arc"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000157630" description="50S ribosomal protein L12">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="73"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="102" mass="10363" checksum="35306FFDE967C52C" modified="1996-10-01" version="1">MEYIYAALLLHSAGKEITEDAIKAVLSAAGVEVDDARVKALVAGLEGVDIEEAIANAAMPVAAAPAAAAPAAAAEEKKEEEKKEEKKEEDTAAVAGLAALFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>P81320</accession>
    <name>YC8C_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1288.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1288.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870804.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81320"/>
    <dbReference type="SMR" id="P81320"/>
    <dbReference type="STRING" id="243232.MJ_1288.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99300">
        <property type="protein sequence ID" value="AAB99300"/>
        <property type="gene ID" value="MJ_1288.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1452189"/>
    <dbReference type="KEGG" id="mja:MJ_1288.1"/>
    <dbReference type="eggNOG" id="arCOG08271">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2021506_0_0_2"/>
    <dbReference type="OMA" id="MHIIAKS"/>
    <dbReference type="OrthoDB" id="113745at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107255" description="Uncharacterized protein MJ1288.1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="123" mass="14404" checksum="3E4A5EFBD75E54B3" modified="1998-07-15" version="1">MHIIAKSILLMAVSFLVIIFTSTIYSELIEIGKYRYIDKVDREITSEVMNAVVLANEGNITLYKKINLNCKVIFENNSFTIIFQNKTYVHKFNNNIRFFKNEISDISKISCKKVNNTYMIYIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q57578</accession>
    <name>Y114_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0114</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0114</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64314">
        <property type="entry name" value="B64314"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869606.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57578"/>
    <dbReference type="EnsemblBacteria" id="AAB98110">
        <property type="protein sequence ID" value="AAB98110"/>
        <property type="gene ID" value="MJ_0114"/>
    </dbReference>
    <dbReference type="GeneID" id="1450956"/>
    <dbReference type="KEGG" id="mja:MJ_0114"/>
    <dbReference type="eggNOG" id="arCOG05023">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_878828_0_0_2"/>
    <dbReference type="OMA" id="NFCILNN"/>
    <dbReference type="OrthoDB" id="46385at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106698" description="Uncharacterized protein MJ0114">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <sequence length="303" mass="34922" checksum="D23B7C090A5837DA" modified="1997-11-01" version="1">MEKVYSKFGRIEDLKEIISGLVNFTGIIRIDNALLFYIDSKLISSKFNGREKSLEEIFSQIPDEFLIEIYQGNEKEVKSALINFKPEESIVEISKLSLVFENEVILNSYNDVYKYLTYINKVIFMPKRFKNEKGIVVYKNKREVFAVYFGRKTLFGKKAISKLKTTFAVSEIIAKIEKISNEELNSLKNQYPEGVLLFGDSINDVVKKIISSKKPIILENVSLIDALSYGTCLIKIEGSEIGYIVAKDGKPVYAFLNDYDGEKSYRLLKSMCIVEDVKYSIYKLSKEEYDMFKTFQENKIPLS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q57592</accession>
    <name>Y128_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ0128</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ0128</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0128</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable antitoxin component of a putative type VII toxin-antitoxin (TA) system. Neutralizes cognate toxic MJ0127 by di-AMPylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Probably forms a complex with cognate toxin MJ0127.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64315">
        <property type="entry name" value="H64315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869621.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57592"/>
    <dbReference type="SMR" id="Q57592"/>
    <dbReference type="STRING" id="243232.MJ_0128"/>
    <dbReference type="EnsemblBacteria" id="AAB98108">
        <property type="protein sequence ID" value="AAB98108"/>
        <property type="gene ID" value="MJ_0128"/>
    </dbReference>
    <dbReference type="GeneID" id="1450970"/>
    <dbReference type="KEGG" id="mja:MJ_0128"/>
    <dbReference type="eggNOG" id="arCOG01206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_10_3_2"/>
    <dbReference type="InParanoid" id="Q57592"/>
    <dbReference type="OMA" id="YMNLKFY"/>
    <dbReference type="OrthoDB" id="108083at2157"/>
    <dbReference type="PhylomeDB" id="Q57592"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106706" description="Putative protein adenylyltransferase MJ0128">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="98" mass="11565" checksum="02ED1E679CBDA3AC" modified="1997-11-01" version="1">MKTISEIKDILRKHKKILKEKYKVKSIAIFGSYARNEQTEKSDIDILVEFYETPDYLKFFELEDYLSDLLGIKVDLVIKGAIKNPYIKKSIEEDLIYV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q57617</accession>
    <name>ACDA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit alpha</fullName>
            <shortName evidence="1">ACDS complex subunit alpha</shortName>
            <ecNumber evidence="1">1.2.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ACDS complex carbon monoxide dehydrogenase subunit alpha</fullName>
            <shortName evidence="1">ACDS CODH subunit alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhA</name>
        <name type="ordered locus">MJ0153</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ACDS complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 7 [4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </cofactor>
        <text evidence="1">Binds 2 [Ni-4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer. Contains two additional 4Fe-4S clusters, dubbed E and F, that probably transport electrons from ferredoxin to the B cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64319">
        <property type="entry name" value="B64319"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869648.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57617"/>
    <dbReference type="SMR" id="Q57617"/>
    <dbReference type="STRING" id="243232.MJ_0153"/>
    <dbReference type="PRIDE" id="Q57617"/>
    <dbReference type="EnsemblBacteria" id="AAB98135">
        <property type="protein sequence ID" value="AAB98135"/>
        <property type="gene ID" value="MJ_0153"/>
    </dbReference>
    <dbReference type="GeneID" id="1450997"/>
    <dbReference type="KEGG" id="mja:MJ_0153"/>
    <dbReference type="eggNOG" id="arCOG02428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_361186_0_0_2"/>
    <dbReference type="InParanoid" id="Q57617"/>
    <dbReference type="OMA" id="LCIGHNV"/>
    <dbReference type="OrthoDB" id="1404at2157"/>
    <dbReference type="PhylomeDB" id="Q57617"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:carbon-monoxide dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01916">
        <property type="entry name" value="ACS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
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        <property type="entry name" value="Prismane-like_a/b-sand"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000155077" description="Acetyl-CoA decarbonylase/synthase complex subunit alpha">
        <location>
            <begin position="1"/>
            <end position="774"/>
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    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="1">
        <location>
            <begin position="398"/>
            <end position="427"/>
        </location>
    </feature>
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        <location>
            <begin position="436"/>
            <end position="466"/>
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    </feature>
    <feature type="binding site" evidence="1">
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            <position position="73"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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            <position position="76"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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            <label>2</label>
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            <position position="77"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
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        <location>
            <position position="79"/>
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        <ligand>
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            <position position="117"/>
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        <ligand>
            <name>CO</name>
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        <location>
            <position position="251"/>
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            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
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            <name>[4Fe-4S] cluster</name>
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            <position position="543"/>
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        <location>
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            <name>[Ni-4Fe-4S] cluster</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01137"/>
        </source>
    </evidence>
    <sequence length="774" mass="85900" checksum="F7CFD66F15CB1C7E" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57649</accession>
    <name>RPO10_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit N</fullName>
            <shortName evidence="3">mjN</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo10</name>
        <name evidence="1" type="synonym">rpoN</name>
        <name type="ordered locus">MJ0196</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Nucleic Acids Res." volume="28" first="4299" last="4305">
            <title>Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12.</title>
            <authorList>
                <person name="Werner F."/>
                <person name="Eloranta J.J."/>
                <person name="Weinzierl R.O."/>
            </authorList>
            <dbReference type="PubMed" id="11058130"/>
            <dbReference type="DOI" id="10.1093/nar/28.21.4299"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the RNA polymerase complex (By similarity). Forms an Rpo3-Rpo10-Rpo11-Rpo12 complex upon coexpression (PubMed:11058130).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869691.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57649"/>
    <dbReference type="SMR" id="Q57649"/>
    <dbReference type="STRING" id="243232.MJ_0196"/>
    <dbReference type="EnsemblBacteria" id="AAB98176">
        <property type="protein sequence ID" value="AAB98176"/>
        <property type="gene ID" value="MJ_0196"/>
    </dbReference>
    <dbReference type="GeneID" id="8803916"/>
    <dbReference type="KEGG" id="mja:MJ_0196"/>
    <dbReference type="eggNOG" id="arCOG04244">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143122_2_1_2"/>
    <dbReference type="InParanoid" id="Q57649"/>
    <dbReference type="OMA" id="YCCRRMF"/>
    <dbReference type="OrthoDB" id="123859at2157"/>
    <dbReference type="PhylomeDB" id="Q57649"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="PTHR23431"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="SSF46924"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000121349" description="DNA-directed RNA polymerase subunit Rpo10">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <sequence length="73" mass="8695" checksum="E716EA406D65B831" modified="1998-12-15" version="2">MMFPIRCFSCGNVIAEVFEEYKERILKGENPKDVLDDLGIKKYCCRRMFISYRIGEDGREIIDEIIAHDERYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q57706</accession>
    <name>CFBE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Coenzyme F(430) synthetase</fullName>
            <ecNumber evidence="1">6.4.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cfbE</name>
        <name type="ordered locus">MJ0258</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the activation the g-propionate side chain of 15,17(3)-seco-F430-17(3)-acid (seco-F430) for intramolecular C-C bond formation to yield the carbocyclic F ring of coenzyme F430.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>15,17(3)-seco-F430-17(3)-acid + ATP = ADP + coenzyme F430 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:52904"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
            <dbReference type="ChEBI" id="CHEBI:136888"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MurCDEF family.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.9" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64332">
        <property type="entry name" value="C64332"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57706"/>
    <dbReference type="SMR" id="Q57706"/>
    <dbReference type="STRING" id="243232.MJ_0258"/>
    <dbReference type="EnsemblBacteria" id="AAB98245">
        <property type="protein sequence ID" value="AAB98245"/>
        <property type="gene ID" value="MJ_0258"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0258"/>
    <dbReference type="eggNOG" id="arCOG02822">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047362_0_0_2"/>
    <dbReference type="InParanoid" id="Q57706"/>
    <dbReference type="OMA" id="VIAPVHC"/>
    <dbReference type="PhylomeDB" id="Q57706"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013221">
        <property type="entry name" value="Mur_ligase_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08245">
        <property type="entry name" value="Mur_ligase_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="SSF53623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106760" description="Coenzyme F(430) synthetase">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="112"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8TJZ6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="404" mass="46913" checksum="C9FDE688807D0746" modified="1996-11-01" version="1">MVFFMLIIDVNHGALTLAEEYLNLGYEVDVWDIYQKIKKSEDFKVKYQKLKEKFGNKLNLFFEQPNFEKYDRVIAPIHCPIDVDFIPFTDAVSKILKEKFGNIHKKIINVTGVKGKTTTTSLINHILKDKYSTYLHNSNFGSIAPPTILKVLNSLDIDKYDFFIFETSLGLIKCKYGAITNVLENYKIAGGRKDALTAKFSSLKNAELSFINKRDINRYDLNINHKCLNVVDVDRAKILDKYPLKFKYFDEIFEFSKNIFGLHFVENSLFAIEICKNLVDMEEIRYRLKTFTIKNRMEIKEINKKILVKNINPGLDVKAISYAIKDFLEVFGGDIYIGGDFGIVCEEIDVKKLSEVLKRFNCRYIFVGEIGKELLNYLNGGYIKSYDENKIKRDSLVILREKIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q57752</accession>
    <name>CAH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable carbonic anhydrase</fullName>
            <shortName>CA</shortName>
            <ecNumber evidence="1">4.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0304</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably reversibly hydrates carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + hydrogencarbonate = CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:10748"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="EC" id="4.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gamma-class carbonic anhydrase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64338">
        <property type="entry name" value="A64338"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869802.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57752"/>
    <dbReference type="SMR" id="Q57752"/>
    <dbReference type="STRING" id="243232.MJ_0304"/>
    <dbReference type="EnsemblBacteria" id="AAB98291">
        <property type="protein sequence ID" value="AAB98291"/>
        <property type="gene ID" value="MJ_0304"/>
    </dbReference>
    <dbReference type="GeneID" id="1451159"/>
    <dbReference type="KEGG" id="mja:MJ_0304"/>
    <dbReference type="eggNOG" id="arCOG01849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064827_4_1_2"/>
    <dbReference type="InParanoid" id="Q57752"/>
    <dbReference type="OMA" id="YVTIGHN"/>
    <dbReference type="OrthoDB" id="84330at2157"/>
    <dbReference type="PhylomeDB" id="Q57752"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="SSF51161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000077472" description="Probable carbonic anhydrase">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P40881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="159" mass="17174" checksum="8EBE072E411BCC94" modified="1996-11-01" version="1">MISKNVRIAKGAVIVGDVTIGDYSSVWYNAVIRGDVDKIIIGNYSNIQDCCVVHCSKGYPTIIGDYVSIGHGAVIHGCRIEDNVLVGMNATILNGAKIGENCIIGANALVTQNKEIPPNSLVLGVPGRVVRELTEEEIKSIKENALRYVKLSETLESYK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57754</accession>
    <name>Y306_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0306</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0306</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64338">
        <property type="entry name" value="C64338"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57754"/>
    <dbReference type="SMR" id="Q57754"/>
    <dbReference type="STRING" id="243232.MJ_0306"/>
    <dbReference type="PRIDE" id="Q57754"/>
    <dbReference type="EnsemblBacteria" id="AAB98298">
        <property type="protein sequence ID" value="AAB98298"/>
        <property type="gene ID" value="MJ_0306"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0306"/>
    <dbReference type="eggNOG" id="arCOG08293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197247_0_0_2"/>
    <dbReference type="OMA" id="ELCIKCE"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106784" description="Uncharacterized protein MJ0306">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <sequence length="78" mass="9301" checksum="AC895EC26103A839" modified="1996-11-01" version="1">MRQSKKDYKLKIFNLVKVMKLRVVCKDENLTDDELCIKCELCIGKDLMTIIEMMNEEYKIDEIIIPNCETLKRILNMD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57777</accession>
    <name>Y331_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0331</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0331</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64341">
        <property type="entry name" value="C64341"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869828.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57777"/>
    <dbReference type="SMR" id="Q57777"/>
    <dbReference type="STRING" id="243232.MJ_0331"/>
    <dbReference type="EnsemblBacteria" id="AAB98319">
        <property type="protein sequence ID" value="AAB98319"/>
        <property type="gene ID" value="MJ_0331"/>
    </dbReference>
    <dbReference type="GeneID" id="1451186"/>
    <dbReference type="KEGG" id="mja:MJ_0331"/>
    <dbReference type="eggNOG" id="arCOG08300">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1811373_0_0_2"/>
    <dbReference type="OMA" id="KENIMTG"/>
    <dbReference type="OrthoDB" id="131011at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006522">
        <property type="entry name" value="Phage_virion_morphogenesis"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05069">
        <property type="entry name" value="Phage_tail_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106800" description="Uncharacterized protein MJ0331">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <sequence length="135" mass="15878" checksum="5ED8E259ECA9D98F" modified="1996-11-01" version="1">MDYKQWVREFKKELAHEIEKELVAEVDDNIQRGFQKKELKWKPLSKDYQKRKEKEGRNTKGLIYHGALLKATDSKVKITSKGLQVKVFNNMVYAGVHEFGSKKKNIPARPFIQPALKKVQKDLPKIVEKVIKRMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57794</accession>
    <name>Y348_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0348</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0348</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64343">
        <property type="entry name" value="D64343"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57794"/>
    <dbReference type="STRING" id="243232.MJ_0348"/>
    <dbReference type="PRIDE" id="Q57794"/>
    <dbReference type="EnsemblBacteria" id="AAB98337">
        <property type="protein sequence ID" value="AAB98337"/>
        <property type="gene ID" value="MJ_0348"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0348"/>
    <dbReference type="eggNOG" id="arCOG09651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_353255_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106819" description="Uncharacterized protein MJ0348">
        <location>
            <begin position="1"/>
            <end position="795"/>
        </location>
    </feature>
    <sequence length="795" mass="92391" checksum="2E782E6A74889639" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="142" xmlns="http://uniprot.org/uniprot">
    <accession>Q57817</accession>
    <name>SECE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secE</name>
        <name type="ordered locus">MJ0371</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Nature" volume="427" first="36" last="44">
            <title>X-ray structure of a protein-conducting channel.</title>
            <authorList>
                <person name="Van den Berg B."/>
                <person name="Clemons W.M. Jr."/>
                <person name="Collinson I."/>
                <person name="Modis Y."/>
                <person name="Hartmann E."/>
                <person name="Harrison S.C."/>
                <person name="Rapoport T.A."/>
            </authorList>
            <dbReference type="PubMed" id="14661030"/>
            <dbReference type="DOI" id="10.1038/nature02218"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 11-66 IN COMPLEX WITH SECY AND SECG</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2007" name="Mol. Cell" volume="26" first="511" last="521">
            <title>The plug domain of the SecY protein stabilizes the closed state of the translocation channel and maintains a membrane seal.</title>
            <authorList>
                <person name="Li W."/>
                <person name="Schulman S."/>
                <person name="Boyd D."/>
                <person name="Erlandson K."/>
                <person name="Beckwith J."/>
                <person name="Rapoport T.A."/>
            </authorList>
            <dbReference type="PubMed" id="17531810"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2007.05.002"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS)</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Mol. Cell" volume="28" first="1083" last="1092">
            <title>Ribosome binding of a single copy of the SecY complex: implications for protein translocation.</title>
            <authorList>
                <person name="Menetret J.F."/>
                <person name="Schaletzky J."/>
                <person name="Clemons W.M. Jr."/>
                <person name="Osborne A.R."/>
                <person name="Skanland S.S."/>
                <person name="Denison C."/>
                <person name="Gygi S.P."/>
                <person name="Kirkpatrick D.S."/>
                <person name="Park E."/>
                <person name="Ludtke S.J."/>
                <person name="Rapoport T.A."/>
                <person name="Akey C.W."/>
            </authorList>
            <dbReference type="PubMed" id="18158904"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2007.10.034"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (9.6 ANGSTROMS) OF 2-433 DOCKED ONTO THE E.COLI RIBOSOME</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2008" name="Structure" volume="16" first="1126" last="1137">
            <title>Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome.</title>
            <authorList>
                <person name="Menetret J.F."/>
                <person name="Hegde R.S."/>
                <person name="Aguiar M."/>
                <person name="Gygi S.P."/>
                <person name="Park E."/>
                <person name="Rapoport T.A."/>
                <person name="Akey C.W."/>
            </authorList>
            <dbReference type="PubMed" id="18611385"/>
            <dbReference type="DOI" id="10.1016/j.str.2008.05.003"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (8.7 ANGSTROMS) OF 3-67 DOCKED ONTO DOG RIBOSOMES</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2009" name="Structure" volume="17" first="1453" last="1464">
            <title>Regulation of the protein-conducting channel by a bound ribosome.</title>
            <authorList>
                <person name="Gumbart J."/>
                <person name="Trabuco L.G."/>
                <person name="Schreiner E."/>
                <person name="Villa E."/>
                <person name="Schulten K."/>
            </authorList>
            <dbReference type="PubMed" id="19913480"/>
            <dbReference type="DOI" id="10.1016/j.str.2009.09.010"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY OF 28-67 DOCKED ONTO E.COLI RIBOSOMES</scope>
    </reference>
    <comment type="function">
        <text>Essential subunit of the protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cell membrane</location>
            <topology>Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64346">
        <property type="entry name" value="C64346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869870.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1RH5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="B=1-74"/>
    </dbReference>
    <dbReference type="PDB" id="1RHZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="B=1-74"/>
    </dbReference>
    <dbReference type="PDB" id="2YXQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="B=1-74"/>
    </dbReference>
    <dbReference type="PDB" id="2YXR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.60 A"/>
        <property type="chains" value="B=1-74"/>
    </dbReference>
    <dbReference type="PDB" id="3BO0">
        <property type="method" value="EM"/>
        <property type="resolution" value="9.60 A"/>
        <property type="chains" value="B=28-67"/>
    </dbReference>
    <dbReference type="PDB" id="3BO1">
        <property type="method" value="EM"/>
        <property type="resolution" value="9.60 A"/>
        <property type="chains" value="B=28-67"/>
    </dbReference>
    <dbReference type="PDB" id="3DKN">
        <property type="method" value="EM"/>
        <property type="resolution" value="8.70 A"/>
        <property type="chains" value="B=3-67"/>
    </dbReference>
    <dbReference type="PDB" id="4V4N">
        <property type="method" value="EM"/>
        <property type="resolution" value="9.00 A"/>
        <property type="chains" value="A7=1-67"/>
    </dbReference>
    <dbReference type="PDB" id="4V7I">
        <property type="method" value="EM"/>
        <property type="chains" value="B=28-67"/>
    </dbReference>
    <dbReference type="PDBsum" id="1RH5"/>
    <dbReference type="PDBsum" id="1RHZ"/>
    <dbReference type="PDBsum" id="2YXQ"/>
    <dbReference type="PDBsum" id="2YXR"/>
    <dbReference type="PDBsum" id="3BO0"/>
    <dbReference type="PDBsum" id="3BO1"/>
    <dbReference type="PDBsum" id="3DKN"/>
    <dbReference type="PDBsum" id="4V4N"/>
    <dbReference type="PDBsum" id="4V7I"/>
    <dbReference type="AlphaFoldDB" id="Q57817"/>
    <dbReference type="SMR" id="Q57817"/>
    <dbReference type="IntAct" id="Q57817">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="Q57817"/>
    <dbReference type="STRING" id="243232.MJ_0371"/>
    <dbReference type="TCDB" id="3.A.5.7.4">
        <property type="family name" value="the general secretory pathway (sec) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98360">
        <property type="protein sequence ID" value="AAB98360"/>
        <property type="gene ID" value="MJ_0371"/>
    </dbReference>
    <dbReference type="GeneID" id="1451228"/>
    <dbReference type="KEGG" id="mja:MJ_0371"/>
    <dbReference type="eggNOG" id="arCOG02204">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191921_0_0_2"/>
    <dbReference type="InParanoid" id="Q57817"/>
    <dbReference type="OMA" id="DFLHQCR"/>
    <dbReference type="OrthoDB" id="121918at2157"/>
    <dbReference type="EvolutionaryTrace" id="Q57817"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="SSF103456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000104219" description="Protein translocase subunit SecE">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="25"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="31"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="14661030"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1RH5"/>
        </source>
    </evidence>
    <sequence length="74" mass="8438" checksum="10474AE938CC0ECD" modified="1996-11-01" version="1">MKTDFNQKIEQLKEFIEECRRVWLVLKKPTKDEYLAVAKVTALGISLLGIIGYIIHVPATYIKGILKPPTTPRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57850</accession>
    <name>NADA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Quinolinate synthase</fullName>
            <ecNumber evidence="1">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadA</name>
        <name type="ordered locus">MJ0407</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dihydroxyacetone phosphate + iminosuccinate = H(+) + 2 H2O + phosphate + quinolinate</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:25889"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64350">
        <property type="entry name" value="G64350"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57850"/>
    <dbReference type="SMR" id="Q57850"/>
    <dbReference type="STRING" id="243232.MJ_0407"/>
    <dbReference type="EnsemblBacteria" id="AAB98397">
        <property type="protein sequence ID" value="AAB98397"/>
        <property type="gene ID" value="MJ_0407"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0407"/>
    <dbReference type="eggNOG" id="arCOG04459">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047382_0_0_2"/>
    <dbReference type="InParanoid" id="Q57850"/>
    <dbReference type="OMA" id="FFLPDKC"/>
    <dbReference type="PhylomeDB" id="Q57850"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD biosynthetic process from aspartate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="PTHR30573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="SSF142754"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155803" description="Quinolinate synthase">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="199"/>
            <end position="201"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <sequence length="307" mass="34863" checksum="C78611F2767C315C" modified="1996-11-01" version="1">MSMDIVERINKLKEEKNAVILAHNYQPKEIQKIADFLGDSLELCIKAKETDADIIVFCGVDFMGESAKILNPEKKVLMPEIEGTQCPMAHQLPPEIIKKYRELYPEAPLVVYVNTTAETKALADITCTSANADRVVNSLDADTVLFGPDNNLAYYVQKRTDKKVIAIPEGGGCYVHKKFTIDDLKRVKSKYPNAKVLIHPECSPELQDNADYIASTSGILRIVLESDDEEFIIGTEVGMINRLEIELEKLGKKKTLIPLRKDAICHEMKRITLEKIEKCLLEERYEIKLEKEIIEKAQKAIERMLRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q57855</accession>
    <name>Y412_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ0412</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0412</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64351">
        <property type="entry name" value="D64351"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57855"/>
    <dbReference type="SMR" id="Q57855"/>
    <dbReference type="STRING" id="243232.MJ_0412"/>
    <dbReference type="PRIDE" id="Q57855"/>
    <dbReference type="EnsemblBacteria" id="AAB98401">
        <property type="protein sequence ID" value="AAB98401"/>
        <property type="gene ID" value="MJ_0412"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0412"/>
    <dbReference type="eggNOG" id="arCOG00193">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q57855"/>
    <dbReference type="OMA" id="YSLFPWR"/>
    <dbReference type="PhylomeDB" id="Q57855"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093219" description="Uncharacterized ABC transporter ATP-binding protein MJ0412">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="17"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="53"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="267" mass="30631" checksum="7FC75A5471AA00EA" modified="1996-11-01" version="1">MEKDDEVKKRDRMKVKLKVENLTKIFEFNGNRVKALDNINLEVYENEFLTVMGPSGCGKTTLLRIIAGLDYPTEGKVLLDGKEVKGPGADRGVVFQQYTLMPWRTVLKNVTFGLELKGIPKNERIEIAKKFIKMVGLEGFEDAYPYQLSGGMQQRVAIARTLANDPEIVLMDEPFAALDAQTRNILQNELLKIWQKEKKTVFFVTHSVDEAVYLSDRVVVLTARPGRIKEIVKIDLERPRDRTSIEFLEYRKKILNILKDEVLKSLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57862</accession>
    <name>Y419_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0419</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0419</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64352">
        <property type="entry name" value="C64352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869918.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57862"/>
    <dbReference type="STRING" id="243232.MJ_0419"/>
    <dbReference type="DNASU" id="1451279"/>
    <dbReference type="EnsemblBacteria" id="AAB98417">
        <property type="protein sequence ID" value="AAB98417"/>
        <property type="gene ID" value="MJ_0419"/>
    </dbReference>
    <dbReference type="GeneID" id="1451279"/>
    <dbReference type="KEGG" id="mja:MJ_0419"/>
    <dbReference type="eggNOG" id="arCOG03206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_764231_0_0_2"/>
    <dbReference type="InParanoid" id="Q57862"/>
    <dbReference type="OMA" id="GPHHIFV"/>
    <dbReference type="OrthoDB" id="51573at2157"/>
    <dbReference type="PhylomeDB" id="Q57862"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002760">
        <property type="entry name" value="O_anti_polymase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01901">
        <property type="entry name" value="O_anti_polymase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106865" description="Uncharacterized protein MJ0419">
        <location>
            <begin position="1"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="278"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="354" mass="40036" checksum="D6C7180C6418A641" modified="1996-11-01" version="1">MLLLHWDNMGKIELHHVFVMLSCIYLIFSDISINSAVVFLFSSIFFYISFTAGKRLYYLIGIDKENLKINLKKHYNFGIFLMIVGLIAVTSDLIWVKDVPLFNPLSRKFLNVYFTTLSHLFLVGWAIVVASSNIDKKKILLYTIIFSILIMLLGYRTNVLVLLISVGAILYYKNKISNREILKYGILVFVILLGLSILRLYALGVEGNPITSRISLTMSIYDIIFNNFNGVFNGYIHYSAVFSYLGLCNGARTVIAKTLGIYNVSITPTIVGAVIGDYGTLAIIPYFGILGIFLGFFYKLAKDVKGIYLGIYGILFAYTLIGIESGILDIDVILYYFFGLILCIYAILLRKLKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57870</accession>
    <name>Y427_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0427</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0427</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64353">
        <property type="entry name" value="C64353"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57870"/>
    <dbReference type="STRING" id="243232.MJ_0427"/>
    <dbReference type="EnsemblBacteria" id="AAB98420">
        <property type="protein sequence ID" value="AAB98420"/>
        <property type="gene ID" value="MJ_0427"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0427"/>
    <dbReference type="eggNOG" id="arCOG08291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656898_0_0_2"/>
    <dbReference type="OMA" id="YLHILRQ"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106872" description="Uncharacterized protein MJ0427">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <sequence length="160" mass="20088" checksum="41557230A5D06B9F" modified="1996-11-01" version="1">MMERMHNLNNVYLKECIHFLDSCINALKEFDLRTFISRFYYGMLYLLNAFEFYIRENIEDWHNKDRYANFSKKIRNFLMDLKLYRHASDYILSPRLEHGKHYEEHWEEFKESYLKLKFFHYLHILRQELYSYRHNQLIAIIIEKLEIIEKLLKLYIMLEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57882</accession>
    <name>Y440_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0440</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0440</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64354">
        <property type="entry name" value="H64354"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57882"/>
    <dbReference type="STRING" id="243232.MJ_0440"/>
    <dbReference type="DNASU" id="1451300"/>
    <dbReference type="EnsemblBacteria" id="AAB98427">
        <property type="protein sequence ID" value="AAB98427"/>
        <property type="gene ID" value="MJ_0440"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0440"/>
    <dbReference type="eggNOG" id="arCOG02177">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086260_0_0_2"/>
    <dbReference type="InParanoid" id="Q57882"/>
    <dbReference type="OMA" id="GYWEQHP"/>
    <dbReference type="PhylomeDB" id="Q57882"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002749">
        <property type="entry name" value="DUF63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700">
        <property type="entry name" value="PTHR40700"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01889">
        <property type="entry name" value="DUF63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106877" description="Uncharacterized protein MJ0440">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="299" mass="34301" checksum="BE64A14E71FFC5B6" modified="1996-11-01" version="1">MYYKLVLSHYSKTSTLINITLIKHLINILRERMIQEIKNFIYKYYIEPAEKGTGYNIVQEITYGIILALALYLFYKALRKLNINIDEKFAIPGIVFTVLIALMRALVDCGYIERSFLTITPGIVFLIGGFFILTILTTGLVFKEKYYKASAVIGLILLLYFLFVFLQHITHLEAILYVGILVGIFYYLVKFLDKTLKLNIIQSKIDDYVVIGQLIDASATTIGIGVYGYWEQHPIPRFLMETFGVYAFIPFKLLIILLALYILNKEVEDENIKNIIKLCIMALGLAPGLRDLFRTVMGV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57886</accession>
    <name>RIO1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>RIO-type serine/threonine-protein kinase Rio1</fullName>
            <ecNumber>2.7.11.1</ecNumber>
            <ecNumber>3.6.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rio1</name>
        <name type="ordered locus">MJ0444</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Despite the protein kinase domain is proposed to act predominantly as an ATPase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1"/>
    <dbReference type="EC" id="3.6.3.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64355">
        <property type="entry name" value="D64355"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57886"/>
    <dbReference type="SMR" id="Q57886"/>
    <dbReference type="STRING" id="243232.MJ_0444"/>
    <dbReference type="EnsemblBacteria" id="AAB98431">
        <property type="protein sequence ID" value="AAB98431"/>
        <property type="gene ID" value="MJ_0444"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0444"/>
    <dbReference type="eggNOG" id="arCOG01180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018693_3_3_2"/>
    <dbReference type="InParanoid" id="Q57886"/>
    <dbReference type="OMA" id="EIRCAKV"/>
    <dbReference type="OrthoDB" id="44076at2157"/>
    <dbReference type="PhylomeDB" id="Q57886"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="SSF56112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000213534" description="RIO-type serine/threonine-protein kinase Rio1">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="76"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2 3">
        <location>
            <position position="214"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="2">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="82"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="G0S3J5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q9BRS2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="290" mass="34469" checksum="52A8045266B2ACAB" modified="1997-11-01" version="1">MSCYKVMPIAKNIDDELYELNKLLSEKEEFQLDREYQKEILEKERKFLEDLKTANEVFDKRTLMTLFSLLAGKHLTEYIGIVNSGKEAVVFKARKGKFYRAVKVYRVATCDFKTMSKYIQGDPRFHLRKSSRRQIIHAWVEKEFRNLRRASEIINAPKARLRRENVLVMDFVGYRGIPAPKLKDMQDLDWEKYFKIIKESMKKLYEEGELVHGDLSEYNILVKDDEPVFIDFSQSVITQHPLAHPLLIRDCINICNFFRRKRVDCNYKDLYKYITGKEIDPIDEAMIKQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57893</accession>
    <name>TRPF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName>PRAI</shortName>
            <ecNumber>5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpF</name>
        <name type="ordered locus">MJ0451</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpF family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64356">
        <property type="entry name" value="C64356"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57893"/>
    <dbReference type="SMR" id="Q57893"/>
    <dbReference type="STRING" id="243232.MJ_0451"/>
    <dbReference type="EnsemblBacteria" id="AAB98440">
        <property type="protein sequence ID" value="AAB98440"/>
        <property type="gene ID" value="MJ_0451"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0451"/>
    <dbReference type="eggNOG" id="arCOG01983">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076364_2_0_2"/>
    <dbReference type="InParanoid" id="Q57893"/>
    <dbReference type="OMA" id="FYAKSPR"/>
    <dbReference type="PhylomeDB" id="Q57893"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="PTHR42894"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154403" description="N-(5'-phosphoribosyl)anthranilate isomerase">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="226" mass="25306" checksum="7B83DC1F16C5957E" modified="1996-11-01" version="1">MGDDLVKVKICGITNEEDIAYISKKVHAVGVIVDVPVKTPRKISLDKAIELKKYVAPFTSLVTVLMPNSIEEVLEIYNALKPNAIQLHGFESLDFVKELNKLKNTGELNAHIIKVIHIPKDEEIDFKTLLNTAKEYEKYVEAILVDTKIESIKLEGKTHNWAVSKKLRESLEKPLILAGGLNKDNVLEAIKTVKPYAIDVSSSLEAYGGKKDLKKVDEFLEVIKKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q57894</accession>
    <name>Y452_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0452</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0452</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64356">
        <property type="entry name" value="D64356"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57894"/>
    <dbReference type="SMR" id="Q57894"/>
    <dbReference type="STRING" id="243232.MJ_0452"/>
    <dbReference type="EnsemblBacteria" id="AAB98441">
        <property type="protein sequence ID" value="AAB98441"/>
        <property type="gene ID" value="MJ_0452"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0452"/>
    <dbReference type="eggNOG" id="arCOG04845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142041_0_0_2"/>
    <dbReference type="InParanoid" id="Q57894"/>
    <dbReference type="OMA" id="HNDAAVN"/>
    <dbReference type="PhylomeDB" id="Q57894"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036608">
        <property type="entry name" value="MTH777-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012033">
        <property type="entry name" value="UCP006600"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09001">
        <property type="entry name" value="DUF1890"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006600">
        <property type="entry name" value="UCP006600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75181">
        <property type="entry name" value="SSF75181"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106882" description="Uncharacterized protein MJ0452">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <sequence length="148" mass="16226" checksum="D468F9C1D0C1E949" modified="1996-11-01" version="1">MMSVLVIVGCPEPPALIPSVLYLTNQLKKKGFNVIIAANPAALKLLEVADDDKYYLKGVGAVDIDGGLRGIEGINKIISFVHNDGGVSYTVTYKAKYNKPTYAIVFGRQINKDYVETLKNSNIGVYTARAFHNPMPIVNRIKEILANL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57909</accession>
    <name>Y485_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable sulfurtransferase</fullName>
            <ecNumber evidence="2">2.8.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0485</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TtcA family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64360">
        <property type="entry name" value="E64360"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869986.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57909"/>
    <dbReference type="SMR" id="Q57909"/>
    <dbReference type="STRING" id="243232.MJ_0485"/>
    <dbReference type="EnsemblBacteria" id="AAB98476">
        <property type="protein sequence ID" value="AAB98476"/>
        <property type="gene ID" value="MJ_0485"/>
    </dbReference>
    <dbReference type="GeneID" id="1451347"/>
    <dbReference type="KEGG" id="mja:MJ_0485"/>
    <dbReference type="eggNOG" id="arCOG00042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830567_0_0_2"/>
    <dbReference type="InParanoid" id="Q57909"/>
    <dbReference type="OMA" id="DECPYSR"/>
    <dbReference type="OrthoDB" id="30824at2157"/>
    <dbReference type="PhylomeDB" id="Q57909"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106892" description="Probable sulfurtransferase">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O58038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="337" mass="39323" checksum="6FB9C66BB619F065" modified="1996-11-01" version="1">MVEINLRELKKYANPFFLTIRKDKILVNNKRMARLSRTKMDKIEEEFGIPVIYSKTYEYVSTKVGRFINKHKIIAPRDIVIVGLSGGKDSLLLLHLLEVYRRKYGIKLIAVTVDVNIGGIRPWKEDTEGVKLIKHHCEMLNVPHIILKNDLDVVELSEILTKHSKGMEFSPCFSCSVIKRHLLGKLAKEIAENENIPYEKVKLAYGHNLDDNSDTILANIFKGERLKFMRPLTRFKYNEVDYQSFKIPLEECIIIRPMLPILERDIIKALEECGIEYYKDKDMCPYSRDRGDSVRRRCHEILEKLEEEIPNIREMVVSSALKTVEYYSKNPYGEDNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q57919</accession>
    <name>Y496_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0496</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0496</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64361">
        <property type="entry name" value="H64361"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869997.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57919"/>
    <dbReference type="STRING" id="243232.MJ_0496"/>
    <dbReference type="EnsemblBacteria" id="AAB98489">
        <property type="protein sequence ID" value="AAB98489"/>
        <property type="gene ID" value="MJ_0496"/>
    </dbReference>
    <dbReference type="GeneID" id="1451358"/>
    <dbReference type="KEGG" id="mja:MJ_0496"/>
    <dbReference type="eggNOG" id="arCOG05032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1821029_0_0_2"/>
    <dbReference type="OMA" id="VGFHKDD"/>
    <dbReference type="OrthoDB" id="105601at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106898" description="Uncharacterized protein MJ0496">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <sequence length="141" mass="15692" checksum="DA284F11A8CEB443" modified="1996-11-01" version="1">MVRVVYVVNLPYKKLGVECDGKQLILKMKSKDVEDSIKMNFESAALLKILMEQSAVIAEKINKDFKKDKIKVHNIHDVGTVFGVDGRVSVGVLPADENRVASILVGFHKDDKHISVVVKPKKIALLSVIITKIISENLKLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57929</accession>
    <name>HIS5_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber>4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>IGP synthase glutaminase subunit</fullName>
            <ecNumber>3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName>IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ImGP synthase subunit HisH</fullName>
            <shortName>IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisH</name>
        <name type="ordered locus">MJ0506</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98496" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.3.2.10"/>
    <dbReference type="EC" id="3.5.1.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98496.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64363">
        <property type="entry name" value="B64363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496519.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57929"/>
    <dbReference type="SMR" id="Q57929"/>
    <dbReference type="STRING" id="243232.MJ_0506"/>
    <dbReference type="MEROPS" id="C26.965"/>
    <dbReference type="EnsemblBacteria" id="AAB98496">
        <property type="protein sequence ID" value="AAB98496"/>
        <property type="gene ID" value="MJ_0506"/>
    </dbReference>
    <dbReference type="GeneID" id="1451368"/>
    <dbReference type="KEGG" id="mja:MJ_0506"/>
    <dbReference type="eggNOG" id="arCOG00089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071837_2_2_2"/>
    <dbReference type="InParanoid" id="Q57929"/>
    <dbReference type="OMA" id="WVYFVHS"/>
    <dbReference type="OrthoDB" id="100772at2157"/>
    <dbReference type="PhylomeDB" id="Q57929"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="PTHR42701"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152458" description="Imidazole glycerol phosphate synthase subunit HisH">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="196" mass="22101" checksum="4D4E20DDE0AA2F5F" modified="2000-12-08" version="2">MIGIIDYNAGNLRSIQKAVELYDKVIITNNSEELLACDKIILPGVGNFGSAMENLAPLKETIYKIVDDRVPFLGICLGMQILFEESEEKRGIKGLGIIKGNVIKFKDVEKLPHMGWNSVKIVKDCPLFEGIKNNSYFYFVHSYHVNPDEDCIVGKTEYGREFPSVINKDNVFATQFHPEKSGKIGLKIIENFVELL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57947</accession>
    <name>Y527_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0527</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0527</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64365">
        <property type="entry name" value="G64365"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870031.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57947"/>
    <dbReference type="STRING" id="243232.MJ_0527"/>
    <dbReference type="EnsemblBacteria" id="AAB98518">
        <property type="protein sequence ID" value="AAB98518"/>
        <property type="gene ID" value="MJ_0527"/>
    </dbReference>
    <dbReference type="GeneID" id="1451392"/>
    <dbReference type="KEGG" id="mja:MJ_0527"/>
    <dbReference type="eggNOG" id="arCOG04828">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1623471_0_0_2"/>
    <dbReference type="OMA" id="FMGNIVC"/>
    <dbReference type="OrthoDB" id="80296at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011314">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaB"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019706">
        <property type="entry name" value="EhaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106918" description="Uncharacterized protein MJ0527">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="164" mass="17616" checksum="BC24D60F9F9B4A60" modified="1996-11-01" version="1">MEIVEIVKIIIAGIICWLNFVLIDTYFGLPEKPGVLGAKTIGEKIRDIGGNLNGGYFMGNIVCSPDASAGTLLASIMNYLMGIEGGFIAALLVWIGNRLCADPGYAGTIGALTITAIIYLLNPIIEAKYFIVGMVLAIFTIQGFEHRYASILLGKIAKKMNRGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57951</accession>
    <name>Y531_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Universal stress protein MJ0531</fullName>
            <shortName>USP MJ0531</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0531</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64366">
        <property type="entry name" value="C64366"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57951"/>
    <dbReference type="SMR" id="Q57951"/>
    <dbReference type="STRING" id="243232.MJ_0531"/>
    <dbReference type="EnsemblBacteria" id="AAB98522">
        <property type="protein sequence ID" value="AAB98522"/>
        <property type="gene ID" value="MJ_0531"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0531"/>
    <dbReference type="eggNOG" id="arCOG02053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049301_11_0_2"/>
    <dbReference type="InParanoid" id="Q57951"/>
    <dbReference type="OMA" id="VSHKVSQ"/>
    <dbReference type="PhylomeDB" id="Q57951"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147445" description="Universal stress protein MJ0531">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="170" mass="18890" checksum="D992BD538494964A" modified="1996-11-01" version="1">MKKVFILLALKYYILNSIIRNGENLYKKIVIPTDGSDVSLEAAKHAINIAKEFDAEVYAIYVVDVSPFVGLPAEGSWELISELLKEEGQEALKKVKKMAEEWGVKIHTEMLEGVPANEIVEFAEKKKADLIVMGTTGKTGLERILLGSVAERVIKNAHCPVLVVKKPKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q57964</accession>
    <name>Y544_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0544</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0544</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64367">
        <property type="entry name" value="H64367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870048.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57964"/>
    <dbReference type="SMR" id="Q57964"/>
    <dbReference type="STRING" id="243232.MJ_0544"/>
    <dbReference type="CAZy" id="GT2">
        <property type="family name" value="Glycosyltransferase Family 2"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98538">
        <property type="protein sequence ID" value="AAB98538"/>
        <property type="gene ID" value="MJ_0544"/>
    </dbReference>
    <dbReference type="GeneID" id="1451409"/>
    <dbReference type="KEGG" id="mja:MJ_0544"/>
    <dbReference type="eggNOG" id="arCOG00895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033536_7_4_2"/>
    <dbReference type="InParanoid" id="Q57964"/>
    <dbReference type="OMA" id="DGDYQHK"/>
    <dbReference type="OrthoDB" id="17570at2157"/>
    <dbReference type="PhylomeDB" id="Q57964"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106922" description="Uncharacterized protein MJ0544">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <sequence length="229" mass="26470" checksum="D2760B2D92FCB26F" modified="1996-11-01" version="1">MIAIIPAFNEEKNILKVLKDLEKLRVDAVVVDDGSKDNTSKIVEEFAKKAKINVYLIRNEKNEGKAKAIEKGTKFALSLNKYKYIIYIDGDYQHKPMDIPKLLKKLEDTNADAVFGIRKYKHIPLHRQISNFFASILTSLAVLIYSKRFYFFRDVQCGFRIIKAEFLKDMKFGDGYAVEHFIALQLAKKGAKIVEEYVSVEYHDEAVSYITTKKILEVAKQVIKFIFLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58017</accession>
    <name>T2M3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II restriction enzyme MjaIII</fullName>
            <shortName>R.MjaIII</shortName>
            <ecNumber>3.1.21.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Endonuclease MjaIII</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Type-2 restriction enzyme MjaIII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaIIIR</name>
        <name type="ordered locus">MJ0600</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="patent" date="1999-03-11" number="WO9911821">
            <title>Method for screening restriction endonucleases.</title>
            <authorList>
                <person name="Noren C.J."/>
                <person name="Roberts R.J."/>
                <person name="Patti J."/>
                <person name="Byrd D.R."/>
                <person name="Morgan R.D."/>
            </authorList>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-GATC-3'; the cleavage site is unknown.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.21.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DpnII type II restriction endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64374">
        <property type="entry name" value="H64374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870104.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58017"/>
    <dbReference type="SMR" id="Q58017"/>
    <dbReference type="STRING" id="243232.MJ_0600"/>
    <dbReference type="REBASE" id="3891">
        <property type="entry name" value="MjaIII"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98591">
        <property type="protein sequence ID" value="AAB98591"/>
        <property type="gene ID" value="MJ_0600"/>
    </dbReference>
    <dbReference type="GeneID" id="1451465"/>
    <dbReference type="KEGG" id="mja:MJ_0600"/>
    <dbReference type="eggNOG" id="arCOG06575">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089327_0_0_2"/>
    <dbReference type="OMA" id="GLEQIFC"/>
    <dbReference type="OrthoDB" id="110729at2157"/>
    <dbReference type="PhylomeDB" id="Q58017"/>
    <dbReference type="PRO" id="PR:Q58017"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021191">
        <property type="entry name" value="Restrct_endonuc_II_DpnII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007637">
        <property type="entry name" value="Restrct_endonuc_II_DpnII-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04556">
        <property type="entry name" value="DpnII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016080">
        <property type="entry name" value="Restrict_endonuc_II_DpmII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000077334" description="Type II restriction enzyme MjaIII">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="290" mass="34392" checksum="ACEBAE31B029900D" modified="1996-11-01" version="1">MNFEYIINSLLETIKTYNFFVDWEKIENNIKKIEKRLHILNYLIGKENFKEEFFELLKEYPEVITVFPILIAVRDNKITILNENMELETLEFKEKKYLTDEEIERYYKFFKETGLEDLLKNRKIKNLVDYVFGVEVGMDTNARKNRIGDLMENIVKKYIENLCKQNKNLDYIFQATKDKIKQKWGINLTLDKTNRKFDFAVFNKNTKKLYLIEVNFYSGGGSKLKATAGEYRSLNEFIKNNNNNVQFIWITDGKGWNTAKNPLKESFNSGVVILNLKMVKEGLLKEILTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q58020</accession>
    <name>GSA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Glutamate-1-semialdehyde 2,1-aminomutase</fullName>
            <shortName>GSA</shortName>
            <ecNumber>5.4.3.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Glutamate-1-semialdehyde aminotransferase</fullName>
            <shortName>GSA-AT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hemL</name>
        <name type="ordered locus">MJ0603</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>(S)-4-amino-5-oxopentanoate = 5-aminolevulinate</text>
            <dbReference type="Rhea" id="RHEA:14265"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="5.4.3.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98593" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="5.4.3.8"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98593.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64375">
        <property type="entry name" value="C64375"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496557.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58020"/>
    <dbReference type="SMR" id="Q58020"/>
    <dbReference type="STRING" id="243232.MJ_0603"/>
    <dbReference type="EnsemblBacteria" id="AAB98593">
        <property type="protein sequence ID" value="AAB98593"/>
        <property type="gene ID" value="MJ_0603"/>
    </dbReference>
    <dbReference type="GeneID" id="1451468"/>
    <dbReference type="KEGG" id="mja:MJ_0603"/>
    <dbReference type="eggNOG" id="arCOG00918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_1_5_2"/>
    <dbReference type="InParanoid" id="Q58020"/>
    <dbReference type="OMA" id="WGPLIFG"/>
    <dbReference type="OrthoDB" id="10264at2157"/>
    <dbReference type="PhylomeDB" id="Q58020"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00317"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042286">
        <property type="term" value="F:glutamate-1-semialdehyde 2,1-aminomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00375">
        <property type="entry name" value="HemL_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004639">
        <property type="entry name" value="4pyrrol_synth_GluAld_NH2Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00713">
        <property type="entry name" value="hemL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000120482" description="Glutamate-1-semialdehyde 2,1-aminomutase">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="426" mass="47451" checksum="DDFFC01FCF52080C" modified="2001-06-01" version="2">MALKMDKSKELFEEAKKYLVGGVNSPVRYFKPYPFFVEKAKDCYLFDVDGNCYIDYCLAYGPMVLGHANDAVIKAVKEQLELGSAYGCPTEKEIILAKEVVKRVPCAEMVRFVNSGTEATMSAIRLARGVTGRKKIIKFDGAYHGAHDYVLVKSGSGALTHGHPNSPGIPEETTKNTILIPFNDEDAVKKAINENKDEIACIIVEPIMGNVGCILPKEGYLEFLREITEENDILLIFDEVITGFRLAKGGAQEYFGVVPDIATLGKILGGGFPIGAIVGRRELMEQFSPLGAIYQAGTFNGNPISITAGIATLKQLDDRFYKETARTAKILADTLRELADKHNIKAKVYNIASMFQIYFNDKEVVNYEIAKQSDTEKFMKYFWRLLEKGVFVPPSQFECCFTSIKHDDEVVDKTIKAMEDVFEGLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58031</accession>
    <name>Y614_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0614</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0614</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64376">
        <property type="entry name" value="F64376"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58031"/>
    <dbReference type="STRING" id="243232.MJ_0614"/>
    <dbReference type="EnsemblBacteria" id="AAB98608">
        <property type="protein sequence ID" value="AAB98608"/>
        <property type="gene ID" value="MJ_0614"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0614"/>
    <dbReference type="eggNOG" id="arCOG04850">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_152996_1_0_2"/>
    <dbReference type="OMA" id="LLICPYG"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002572">
        <property type="entry name" value="DUF22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01629">
        <property type="entry name" value="DUF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106959" description="Uncharacterized protein MJ0614">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <sequence length="130" mass="14873" checksum="311EF0525908896F" modified="1996-11-01" version="1">MVMFMVFRILGRMTKIEKEIKEEEAKYDLIIKNEAKIEPIVAEEDMEFKQGDIKPIRIKKIKIPPMSVLLICPYGRHRVGHVVAVGEEVPMPIDVEREVDMAMFACGFEGEVKKGDLIGMLLILAAEKRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58043</accession>
    <name>PURO_METJA</name>
    <protein>
        <recommendedName>
            <fullName>IMP cyclohydrolase</fullName>
            <ecNumber>3.5.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>IMP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Inosinicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">purO</name>
        <name type="ordered locus">MJ0626</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Bacteriol." volume="184" first="1471" last="1473">
            <title>New class of IMP cyclohydrolases in Methanococcus jannaschii.</title>
            <authorList>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="11844782"/>
            <dbReference type="DOI" id="10.1128/JB.184.5.1471-1473.2002"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="function">
        <text>Catalyzes the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>H2O + IMP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 6-8.5.</text>
        </phDependence>
        <temperatureDependence>
            <text>Fully active at 80 degrees Celsius. Gradually loses some activity from 90 to 100 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal IMP cyclohydrolase family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98620" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.5.4.10"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98620.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64378">
        <property type="entry name" value="B64378"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496562.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58043"/>
    <dbReference type="SMR" id="Q58043"/>
    <dbReference type="STRING" id="243232.MJ_0626"/>
    <dbReference type="EnsemblBacteria" id="AAB98620">
        <property type="protein sequence ID" value="AAB98620"/>
        <property type="gene ID" value="MJ_0626"/>
    </dbReference>
    <dbReference type="GeneID" id="1451492"/>
    <dbReference type="KEGG" id="mja:MJ_0626"/>
    <dbReference type="eggNOG" id="arCOG04727">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1352116_0_0_2"/>
    <dbReference type="InParanoid" id="Q58043"/>
    <dbReference type="OMA" id="HVDPIAE"/>
    <dbReference type="OrthoDB" id="106750at2157"/>
    <dbReference type="PhylomeDB" id="Q58043"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14617"/>
    <dbReference type="BRENDA" id="3.5.4.10">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00705">
        <property type="entry name" value="IMP_cyclohydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010191">
        <property type="entry name" value="IMP_cyclohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020600">
        <property type="entry name" value="IMP_cyclohydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036795">
        <property type="entry name" value="IMP_cyclohydrolase-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07826">
        <property type="entry name" value="IMP_cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004866">
        <property type="entry name" value="IMP_cclhdr_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75569">
        <property type="entry name" value="SSF75569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01922">
        <property type="entry name" value="purO_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145795" description="IMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="202" mass="22941" checksum="339DDF505D0733D9" modified="2003-09-09" version="2">MYIGRFLVVGKTKEGKPFAAYRVSSRSFPNREAKKMDDNTVAIIPKDLNEMFKNPYITYNCIKVIDKTIVVSNGTHTDFIAEKLHFGKRDALAYVLAVMDYEKDDYKTPRIAAILDENECYMGYVAHDDIRVKKVELKDGKGYYLGVYNACKIDENQIIDIKGETAEEIADYILNYEEFEHPVACAVAVIDKDGIKIATKGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58057</accession>
    <name>Y640_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0640</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0640</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64379">
        <property type="entry name" value="H64379"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870145.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58057"/>
    <dbReference type="SMR" id="Q58057"/>
    <dbReference type="STRING" id="243232.MJ_0640"/>
    <dbReference type="EnsemblBacteria" id="AAB98632">
        <property type="protein sequence ID" value="AAB98632"/>
        <property type="gene ID" value="MJ_0640"/>
    </dbReference>
    <dbReference type="GeneID" id="1451506"/>
    <dbReference type="KEGG" id="mja:MJ_0640"/>
    <dbReference type="eggNOG" id="arCOG00640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073250_0_0_2"/>
    <dbReference type="InParanoid" id="Q58057"/>
    <dbReference type="OMA" id="VPMVGGH"/>
    <dbReference type="OrthoDB" id="57531at2157"/>
    <dbReference type="PhylomeDB" id="Q58057"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02192">
        <property type="entry name" value="PurM-like3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017668">
        <property type="entry name" value="Methan_mark_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011413">
        <property type="entry name" value="UCP036540_AIR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036540">
        <property type="entry name" value="UCP036540_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03267">
        <property type="entry name" value="methan_mark_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106967" description="Uncharacterized protein MJ0640">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <sequence length="324" mass="35805" checksum="9DDA077ED84982EE" modified="1996-11-01" version="1">MNLKKVVNEIRNFEGILRKIAIKDVVETFDFNDEDYEFDIIVDFGDDAAVIGIDGDNAILLAADGIWGKLLEADPWWAGYCSVLVNCKDIAAMGGKCVGMTNIISIKDKDICREVLKGVKDGVKKFGVPMVGGHTHPDAMCNVLDVSITGIAKKDCILRSDNAKIGDKIIFAYDLVGQIYKSFPLNWDTTTMKSKKLVRAQMDALVQIAENKLANSCKDISNPGAIGTLGMLLEVSRKGGVVDITKIPKPEEIDLIHWLKVYPGSGYVLTAKEENFKEIKDIFEDVEMTAEICGEVIAEKKLYITDGENKEVVFDFEKEFICGC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58080</accession>
    <name>Y666_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative molybdopterin biosynthesis protein MJ0666</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0666</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text>Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoeA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64383">
        <property type="entry name" value="B64383"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870171.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58080"/>
    <dbReference type="SMR" id="Q58080"/>
    <dbReference type="STRING" id="243232.MJ_0666"/>
    <dbReference type="EnsemblBacteria" id="AAB98661">
        <property type="protein sequence ID" value="AAB98661"/>
        <property type="gene ID" value="MJ_0666"/>
    </dbReference>
    <dbReference type="GeneID" id="1451532"/>
    <dbReference type="KEGG" id="mja:MJ_0666"/>
    <dbReference type="eggNOG" id="arCOG00216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010186_7_2_2"/>
    <dbReference type="InParanoid" id="Q58080"/>
    <dbReference type="OMA" id="MTGAMVP"/>
    <dbReference type="OrthoDB" id="51611at2157"/>
    <dbReference type="PhylomeDB" id="Q58080"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018315">
        <property type="term" value="P:molybdenum incorporation into molybdenum-molybdopterin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="PTHR10192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="SSF63867"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="SSF63882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000171004" description="Putative molybdopterin biosynthesis protein MJ0666">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="398" mass="44418" checksum="81747A9908607ACB" modified="1996-11-01" version="1">MKLIKNLMPLKSAEKIVFEKLSEYLDENKKVKEVDIVEALNRISAEDIKAPIDLPYFNKAAMDGYAVIAEDTFGASETNPIILNLADGDEITYGEAKKIFTGDKLPKNANAVVMKEFCNEVDDFVEVYKTVHPNENVSRIGEDVKKGDVVLKKGEIINPYHLNMLASLGIKKIKVYDLSFGIISTGDELINLDEIRDIEEDISKLDGKIINSNSYMLYGLVKNLGFNAKIYDIVKDDKEKLKKAIKTALSENDALLITGGTSVSERDITVETVRELGDVIVHGVNIRPGKPFGFGIINDKPVFMLSGYPVASAVQFELFIQRFFIERKKVTLPLKRNMASELGRVDFVRVKVDIEVEPIRITGSGVISSLIKSDGYILIPENVEGYEKGELVDVYLLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58086</accession>
    <name>Y672_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter MJ0672</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0672</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CitM (TC 2.A.11) transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64383">
        <property type="entry name" value="H64383"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870177.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58086"/>
    <dbReference type="SMR" id="Q58086"/>
    <dbReference type="STRING" id="243232.MJ_0672"/>
    <dbReference type="TCDB" id="2.A.47.5.1">
        <property type="family name" value="the divalent anion:na(+) symporter (dass) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98666">
        <property type="protein sequence ID" value="AAB98666"/>
        <property type="gene ID" value="MJ_0672"/>
    </dbReference>
    <dbReference type="GeneID" id="1451538"/>
    <dbReference type="KEGG" id="mja:MJ_0672"/>
    <dbReference type="eggNOG" id="arCOG00237">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005170_0_2_2"/>
    <dbReference type="InParanoid" id="Q58086"/>
    <dbReference type="OMA" id="LHGMNTY"/>
    <dbReference type="OrthoDB" id="49249at2157"/>
    <dbReference type="PhylomeDB" id="Q58086"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098656">
        <property type="term" value="P:anion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031312">
        <property type="entry name" value="Na/sul_symport_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001898">
        <property type="entry name" value="SLC13A/DASS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00939">
        <property type="entry name" value="Na_sulph_symp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00785">
        <property type="entry name" value="dass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01271">
        <property type="entry name" value="NA_SULFATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000172507" description="Uncharacterized transporter MJ0672">
        <location>
            <begin position="1"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="326"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="379"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="432" mass="47542" checksum="A24BF9E64475CB74" modified="1996-11-01" version="1">MRLSKEFIGLGIITASLIFGSSLPDIYKGIVILIVAGCLWFFELLPLPVTSLAIPIMAVFLGIFNLKEALTYFAHPIIFLFLGGFMLAQALKNHNLDKFIAYKLLNYGKDFKTTCFLMFLSAYFLSMWISNTSATLILLPIALGLLHKKTGKLRDFLLLGVAYSASIGGIATIIGSPPNAIASSYLDYGFFSWFKVGFPISLLLFLICTLTLYIYFKKWIPKEDIAIQARMELSRNAYKLLVIFVLIASLWIISDYLSEIFNVQYFDSVIAIFAIILLFVFNLVEVNDFKKIDWGTLILFGGALCLGGVIVKSGANTFLSEKLIAILGNLTPIVLLFLVVTITIILTNFISNTGLTGIIVPILFGVSLGIPKEILILAVGMSASCSFILPVGTPPNAIVYSEGVKKEEMMKIGMILSILSAAVITLYSILYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58101</accession>
    <name>Y688_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0146 protein MJ0688</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0688</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0146 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98683" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98683.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496579.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58101"/>
    <dbReference type="SMR" id="Q58101"/>
    <dbReference type="STRING" id="243232.MJ_0688"/>
    <dbReference type="EnsemblBacteria" id="AAB98683">
        <property type="protein sequence ID" value="AAB98683"/>
        <property type="gene ID" value="MJ_0688"/>
    </dbReference>
    <dbReference type="GeneID" id="1451554"/>
    <dbReference type="KEGG" id="mja:MJ_0688"/>
    <dbReference type="eggNOG" id="arCOG04385">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_148458_0_0_2"/>
    <dbReference type="InParanoid" id="Q58101"/>
    <dbReference type="OMA" id="LIYSIRP"/>
    <dbReference type="OrthoDB" id="92832at2157"/>
    <dbReference type="PhylomeDB" id="Q58101"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00341">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005353">
        <property type="entry name" value="UPF0146"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03686">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016725">
        <property type="entry name" value="UCP016725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145094" description="UPF0146 protein MJ0688">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="137" mass="16008" checksum="27D3948A98FACB66" modified="2001-04-27" version="3">MNVKIIVEFIKKFAEENNCKKIAEIGIGFKFDVARELSKYFDLIAIDINEKAIEKAKLLGLNAYKDDLFNPNISLYKNIDLIYSIRPPRDLQPYILDLSKKVNANLIIRPLLNEMPIKELKLKNYKGEVFYIKEKQI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58111</accession>
    <name>Y700_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized MscS family protein MJ0700</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0700</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64387">
        <property type="entry name" value="D64387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870205.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58111"/>
    <dbReference type="SMR" id="Q58111"/>
    <dbReference type="STRING" id="243232.MJ_0700"/>
    <dbReference type="EnsemblBacteria" id="AAB98693">
        <property type="protein sequence ID" value="AAB98693"/>
        <property type="gene ID" value="MJ_0700"/>
    </dbReference>
    <dbReference type="GeneID" id="1451567"/>
    <dbReference type="KEGG" id="mja:MJ_0700"/>
    <dbReference type="eggNOG" id="arCOG01569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066007_1_0_2"/>
    <dbReference type="InParanoid" id="Q58111"/>
    <dbReference type="OMA" id="IMEIREW"/>
    <dbReference type="OrthoDB" id="57211at2157"/>
    <dbReference type="PhylomeDB" id="Q58111"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000110255" description="Uncharacterized MscS family protein MJ0700">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="324" mass="37260" checksum="E3A76D3F1F5BAEB4" modified="1996-11-01" version="1">MIDNKLKLVIKVVLLIFLLHSLMSIFNLETYIKILAKYQNQIMIIAIIILSGLIIVDIASEVFKKYARTREEKAGEYLTLNYIFKYLVYVCVTLMIFGVIYQNVSSLVVSVGLIGAAVTYALQKPILNFAGWIIILYTRTIKIGDRIFIKNMGAGDVFDIDTQHIYLSELTTDTFEPTGRVLIIPNSYIFTASIENLTKGSPYIWDNIVVHFTLNSNINKAEKIVFESADEVIGNLMRELYEKWSKRKYLISRKLSDKPVVRVGITRSSFYIKALYLVNVYEKAKIRSEINKRILEKVNKENDVKLAYPHIKAVIETENKFKNT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58136</accession>
    <name>Y726_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0726</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0726</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the FrhG family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64390">
        <property type="entry name" value="F64390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870231.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58136"/>
    <dbReference type="SMR" id="Q58136"/>
    <dbReference type="STRING" id="243232.MJ_0726"/>
    <dbReference type="EnsemblBacteria" id="AAB98722">
        <property type="protein sequence ID" value="AAB98722"/>
        <property type="gene ID" value="MJ_0726"/>
    </dbReference>
    <dbReference type="GeneID" id="1451603"/>
    <dbReference type="KEGG" id="mja:MJ_0726"/>
    <dbReference type="eggNOG" id="arCOG02473">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075477_0_0_2"/>
    <dbReference type="OMA" id="INDEFCT"/>
    <dbReference type="OrthoDB" id="71699at2157"/>
    <dbReference type="PhylomeDB" id="Q58136"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159235" description="Uncharacterized protein MJ0726">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="160"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="216" mass="23789" checksum="2FD9F067698982FE" modified="1996-11-01" version="1">MTGCGSCGKIIKNIEKKYYNQLKEKDIVLVGGAVNLDDEEEVKKIMEIRKNSKVLIAVGSCAVSGGFQRMLIGLENGFPQRFVRIGDVVKVDYAIIGCPPDEEEVERIVKAVIEKDKEIVDSYLILKPYEVIAGKPIIDAYMKVNDVLLTSNKELCLGCDDKPINDEFCTGCGTCVAKCPANALTIDEKPKVNISKCIKCGTCFFNCIRVKEALLP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58145</accession>
    <name>RUBR1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable Rubredoxin-1</fullName>
            <shortName>RD 1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0735</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the rubredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64391">
        <property type="entry name" value="G64391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870240.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58145"/>
    <dbReference type="SMR" id="Q58145"/>
    <dbReference type="STRING" id="243232.MJ_0735"/>
    <dbReference type="EnsemblBacteria" id="AAB98730">
        <property type="protein sequence ID" value="AAB98730"/>
        <property type="gene ID" value="MJ_0735"/>
    </dbReference>
    <dbReference type="GeneID" id="8512767"/>
    <dbReference type="KEGG" id="mja:MJ_0735"/>
    <dbReference type="eggNOG" id="arCOG04391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128747_1_0_2"/>
    <dbReference type="InParanoid" id="Q58145"/>
    <dbReference type="OMA" id="MSAYRCP"/>
    <dbReference type="OrthoDB" id="126197at2157"/>
    <dbReference type="PhylomeDB" id="Q58145"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043448">
        <property type="term" value="P:alkane catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000135058" description="Probable Rubredoxin-1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Rubredoxin-like" evidence="2">
        <location>
            <begin position="19"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="80" mass="9297" checksum="27D329FD6B608D09" modified="1996-11-01" version="1">MVELKIACKLDGSCEKPRYRKYKCKVCGWVYDPLKGDPSQNIPPKTPFEELPDTWICPVCRGKVGKESFEPLDEWVEFDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58163</accession>
    <name>Y753_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0753</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0753</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64394">
        <property type="entry name" value="A64394"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58163"/>
    <dbReference type="SMR" id="Q58163"/>
    <dbReference type="STRING" id="243232.MJ_0753"/>
    <dbReference type="EnsemblBacteria" id="AAB98755">
        <property type="protein sequence ID" value="AAB98755"/>
        <property type="gene ID" value="MJ_0753"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0753"/>
    <dbReference type="eggNOG" id="arCOG05048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1485903_0_0_2"/>
    <dbReference type="OMA" id="CGRYIDT"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013994" description="Uncharacterized protein MJ0753">
        <location>
            <begin position="25"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="210" mass="23606" checksum="F17A00F449E214B2" modified="1996-11-01" version="1" precursor="true">MKNMKIINKIVAILLLFSILSLSFAWNDCPYGRVNCTYPGECGRYIDTNHNGICDHSEPPPTTTIKTTNNEEEIKTSNVSSLELTEELINEYVGISGKELKSYTIKQVCDKYGISPKCLKEKLNINVPDDTTFGEIKEVYGIPPSVIKKAIVECMIEEGKIKLNTTNTIDNNRDLNNNNSGNEKVGNTILDKIVSFLFSTINLRDLLFKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58197</accession>
    <name>Y787_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0787</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0787</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH1137.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64398">
        <property type="entry name" value="C64398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870294.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58197"/>
    <dbReference type="STRING" id="243232.MJ_0787"/>
    <dbReference type="EnsemblBacteria" id="AAB98783">
        <property type="protein sequence ID" value="AAB98783"/>
        <property type="gene ID" value="MJ_0787"/>
    </dbReference>
    <dbReference type="GeneID" id="1451666"/>
    <dbReference type="KEGG" id="mja:MJ_0787"/>
    <dbReference type="eggNOG" id="arCOG04861">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_539284_0_0_2"/>
    <dbReference type="OMA" id="HHGYMCG"/>
    <dbReference type="OrthoDB" id="13657at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016760">
        <property type="entry name" value="MonoFe_hyd_HmdC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10113">
        <property type="entry name" value="Fibrillarin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019375">
        <property type="entry name" value="UCP019375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03958">
        <property type="entry name" value="monoFe_hyd_HmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107037" description="Uncharacterized protein MJ0787">
        <location>
            <begin position="1"/>
            <end position="504"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="504" mass="56128" checksum="1F6C18C2C2655EC0" modified="1996-11-01" version="1">MRELIKEAVNSLDSALELRKLIIKKLNEKKLKESDIIEIVDAVDDLSLEEIQKLGSNLRTFPMGCDLVEIAVGPCSSSLTLIQFIENCILTDYMGFPIHICSYAVADIAEKEGLKPIEVLKMVLENVDVPIDIDHFGMYGPMRFPKEITHCYGDCYFKGPPFKGCPRNRIHKRLIEKEKEHADEFEDWIKLASTLCINVVEEQGGEEHAAPLEEMKIVAETAKKYGKGLEGIFHIGDGYDDLITGIKACIDLDVDVFVVEGAPFNRAKNRLKAFAKAIAVSRILVKGGVVATNGAYEDECRVGLRSGLNTILTGFPLNHHGYMCGYSPKTAKRGNFGLRRVMRIIKEEIRAGNVNATFIDKDMVKAIALGNRFLKGNIYPYSIGGFYLGDAHWAAIKESNLCKKLKINKTIDDISAEKVGLIGGRYISWAIAEKAEEVYISDTDSWVEKATIKILNEAGINAYPCNGDDKKVLEADKAYITTFIPNIALKILNKLRDSKVELLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58198</accession>
    <name>Y788_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0788</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0788</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64398">
        <property type="entry name" value="D64398"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58198"/>
    <dbReference type="STRING" id="243232.MJ_0788"/>
    <dbReference type="EnsemblBacteria" id="AAB98787">
        <property type="protein sequence ID" value="AAB98787"/>
        <property type="gene ID" value="MJ_0788"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0788"/>
    <dbReference type="eggNOG" id="arCOG06614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2597743_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107039" description="Uncharacterized protein MJ0788">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <sequence length="79" mass="9582" checksum="5DDF8E58CDAF1865" modified="1996-11-01" version="1">MVKLMDIDEFLNYLSNATKENFKKTKHFEIRIELREDNIPNEEELFEILTKNKPVGILKQKDDKFLRYIMSLMKNMMLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58258</accession>
    <name>MTRD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit D</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrD</name>
        <name type="ordered locus">MJ0848</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrD family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64405">
        <property type="entry name" value="H64405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870362.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58258"/>
    <dbReference type="STRING" id="243232.MJ_0848"/>
    <dbReference type="EnsemblBacteria" id="AAB98853">
        <property type="protein sequence ID" value="AAB98853"/>
        <property type="gene ID" value="MJ_0848"/>
    </dbReference>
    <dbReference type="GeneID" id="1451736"/>
    <dbReference type="KEGG" id="mja:MJ_0848"/>
    <dbReference type="eggNOG" id="arCOG04869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1109510_0_0_2"/>
    <dbReference type="InParanoid" id="Q58258"/>
    <dbReference type="OMA" id="HDPKFKR"/>
    <dbReference type="OrthoDB" id="95538at2157"/>
    <dbReference type="PhylomeDB" id="Q58258"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01097">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005779">
        <property type="entry name" value="MeTrfase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04207">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016552">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01112">
        <property type="entry name" value="mtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147530" description="Tetrahydromethanopterin S-methyltransferase subunit D">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="230" mass="22879" checksum="B9D10D88DC6B64F3" modified="1997-11-01" version="1">MDIVSAIVPLIEMTIAGAIINASVHFIPVGGAPAAMATSTGVGTGTTQLAAGAGFTGLMGAAVMASNVGLSPIGMALIMISGAVSSMIMLGVTMLIGQLIYVFGVGVVPAADKCEIDPITKDPQKPYVTPGTTGHGVPTVCFVSGLIGAALGGIGGALAYIALRKLGLDPGVAGMLAVGFFFINAVLASYNIGGTIEGFHDPKFKKMPNGVIASTVASLLFGIISVLMVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58260</accession>
    <name>MTRB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit B</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrB</name>
        <name type="ordered locus">MJ0850</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64406">
        <property type="entry name" value="B64406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870364.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58260"/>
    <dbReference type="IntAct" id="Q58260">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="MINT" id="Q58260"/>
    <dbReference type="STRING" id="243232.MJ_0850"/>
    <dbReference type="EnsemblBacteria" id="AAB98855">
        <property type="protein sequence ID" value="AAB98855"/>
        <property type="gene ID" value="MJ_0850"/>
    </dbReference>
    <dbReference type="GeneID" id="1451738"/>
    <dbReference type="KEGG" id="mja:MJ_0850"/>
    <dbReference type="eggNOG" id="arCOG04867">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171544_0_0_2"/>
    <dbReference type="InParanoid" id="Q58260"/>
    <dbReference type="OMA" id="NSFPNRD"/>
    <dbReference type="OrthoDB" id="114178at2157"/>
    <dbReference type="PhylomeDB" id="Q58260"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01094">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008690">
        <property type="entry name" value="MtrB_MeTrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05440">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005518">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04166">
        <property type="entry name" value="methano_MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147514" description="Tetrahydromethanopterin S-methyltransferase subunit B">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="103" mass="11527" checksum="0797F2691A1CE10F" modified="1997-11-01" version="1">MATYVFIDQNIPLVYTVETGVITKGFGDLLFVDVSPIEEQIKKLETLVDAYEHSLDPRYPPLNSFPNRDGVYAISGYFKSAFFGFWIGLGIMALLAIILGVKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58263</accession>
    <name>MTRG_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrahydromethanopterin S-methyltransferase subunit G</fullName>
            <ecNumber evidence="1">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtrG</name>
        <name type="ordered locus">MJ0853</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtrG family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64406">
        <property type="entry name" value="E64406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870367.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58263"/>
    <dbReference type="SMR" id="Q58263"/>
    <dbReference type="STRING" id="243232.MJ_0853"/>
    <dbReference type="EnsemblBacteria" id="AAB98858">
        <property type="protein sequence ID" value="AAB98858"/>
        <property type="gene ID" value="MJ_0853"/>
    </dbReference>
    <dbReference type="GeneID" id="1451741"/>
    <dbReference type="KEGG" id="mja:MJ_0853"/>
    <dbReference type="eggNOG" id="arCOG03380">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_191926_0_0_2"/>
    <dbReference type="InParanoid" id="Q58263"/>
    <dbReference type="OMA" id="PQVIMDP"/>
    <dbReference type="OrthoDB" id="130315at2157"/>
    <dbReference type="PhylomeDB" id="Q58263"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01500">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005866">
        <property type="entry name" value="THM_MeTrfase_su_G"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04210">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006500">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01149">
        <property type="entry name" value="mtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147555" description="Tetrahydromethanopterin S-methyltransferase subunit G">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01500"/>
        </source>
    </evidence>
    <sequence length="84" mass="9449" checksum="4802F872BAD32A2E" modified="1997-11-01" version="1">MSEDEKLPQVIMDPADYEALKKRLDELEKKVENTNAELFQLAGKKVGRDIGILYGLVIGIILSYILPALIKIIQILSLKVLVQQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58265</accession>
    <name>Y855_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0855</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0855</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64406">
        <property type="entry name" value="G64406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870369.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58265"/>
    <dbReference type="EnsemblBacteria" id="AAB98862">
        <property type="protein sequence ID" value="AAB98862"/>
        <property type="gene ID" value="MJ_0855"/>
    </dbReference>
    <dbReference type="GeneID" id="1451743"/>
    <dbReference type="KEGG" id="mja:MJ_0855"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1178130_0_0_2"/>
    <dbReference type="InParanoid" id="Q58265"/>
    <dbReference type="OMA" id="CYLTAGF"/>
    <dbReference type="OrthoDB" id="114615at2157"/>
    <dbReference type="PhylomeDB" id="Q58265"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107079" description="Uncharacterized protein MJ0855">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <sequence length="228" mass="26405" checksum="0064010139B99F9A" modified="1996-11-01" version="1">MTNAMKIIEMLRIIDNRAKFMGIKLTMMKNLLEKYKDNKELLKEVLKLTEGTRLHELILEAYPPLEELKKEIREEEHKIKITSESGGEEKKEFCTFEGPVSLIAYIKEYLRKYYLGNNVKRIFYDIGKDYAIKLGINTYDDMITFMKKDFGEVVIEKSEPLTVVVKDNKECKNCKASEPICYLTAGFIAGCLENMTNKTYIVEVTEEKCQAVGDPYCTFVAKKSIRLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58293</accession>
    <name>TRM5B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>tRNA (guanine(37)-N1)-methyltransferase Trm5b</fullName>
            <ecNumber evidence="2">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">trm5b</name>
        <name type="ordered locus">MJ0883</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Mol. Biol." volume="339" first="707" last="719">
            <title>Distinct origins of tRNA(m1G37) methyltransferase.</title>
            <authorList>
                <person name="Christian T."/>
                <person name="Evilia C."/>
                <person name="Williams S."/>
                <person name="Hou Y.M."/>
            </authorList>
            <dbReference type="PubMed" id="15165845"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2004.04.025"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2006" name="Biochemistry" volume="45" first="7463" last="7473">
            <title>Catalysis by the second class of tRNA(m1G37) methyl transferase requires a conserved proline.</title>
            <authorList>
                <person name="Christian T."/>
                <person name="Evilia C."/>
                <person name="Hou Y.M."/>
            </authorList>
            <dbReference type="PubMed" id="16768442"/>
            <dbReference type="DOI" id="10.1021/bi0602314"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF ARG-145; TYR-177; GLY-205; GLY-207; ASP-223; ASN-225; PRO-226; ASN-265 AND PRO-267</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="RNA" volume="16" first="2484" last="2492">
            <title>Mechanism of N-methylation by the tRNA m1G37 methyltransferase Trm5.</title>
            <authorList>
                <person name="Christian T."/>
                <person name="Lahoud G."/>
                <person name="Liu C."/>
                <person name="Hoffmann K."/>
                <person name="Perona J.J."/>
                <person name="Hou Y.M."/>
            </authorList>
            <dbReference type="PubMed" id="20980671"/>
            <dbReference type="DOI" id="10.1261/rna.2376210"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2008" name="Proteins" volume="72" first="1274" last="1289">
            <title>Crystal structure of archaeal tRNA(m(1)G37)methyltransferase aTrm5.</title>
            <authorList>
                <person name="Goto-Ito S."/>
                <person name="Ito T."/>
                <person name="Ishii R."/>
                <person name="Muto Y."/>
                <person name="Bessho Y."/>
                <person name="Yokoyama S."/>
            </authorList>
            <dbReference type="PubMed" id="18384044"/>
            <dbReference type="DOI" id="10.1002/prot.22019"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference evidence="8 9" key="6">
        <citation type="journal article" date="2009" name="Nat. Struct. Mol. Biol." volume="16" first="1109" last="1115">
            <title>Tertiary structure checkpoint at anticodon loop modification in tRNA functional maturation.</title>
            <authorList>
                <person name="Goto-Ito S."/>
                <person name="Ito T."/>
                <person name="Kuratani M."/>
                <person name="Bessho Y."/>
                <person name="Yokoyama S."/>
            </authorList>
            <dbReference type="PubMed" id="19749755"/>
            <dbReference type="DOI" id="10.1038/nsmb.1653"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.65 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE AND TRNA</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 6">Specifically methylates the N1 position of guanosine-37 in various tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">1 uM for S-adenosyl-L-methionine</KM>
            <KM evidence="3">0.7 uM for tRNA</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="2 4 5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64410">
        <property type="entry name" value="C64410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870397.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2YX1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-336"/>
    </dbReference>
    <dbReference type="PDB" id="2ZZM">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.65 A"/>
        <property type="chains" value="A=1-336"/>
    </dbReference>
    <dbReference type="PDB" id="2ZZN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.95 A"/>
        <property type="chains" value="A/B=1-336"/>
    </dbReference>
    <dbReference type="PDB" id="3AY0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.05 A"/>
        <property type="chains" value="A/B=1-336"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YX1"/>
    <dbReference type="PDBsum" id="2ZZM"/>
    <dbReference type="PDBsum" id="2ZZN"/>
    <dbReference type="PDBsum" id="3AY0"/>
    <dbReference type="AlphaFoldDB" id="Q58293"/>
    <dbReference type="SMR" id="Q58293"/>
    <dbReference type="STRING" id="243232.MJ_0883"/>
    <dbReference type="EnsemblBacteria" id="AAB98887">
        <property type="protein sequence ID" value="AAB98887"/>
        <property type="gene ID" value="MJ_0883"/>
    </dbReference>
    <dbReference type="GeneID" id="1451772"/>
    <dbReference type="KEGG" id="mja:MJ_0883"/>
    <dbReference type="eggNOG" id="arCOG00033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_1_2"/>
    <dbReference type="InParanoid" id="Q58293"/>
    <dbReference type="OMA" id="HYYDIQH"/>
    <dbReference type="OrthoDB" id="55502at2157"/>
    <dbReference type="PhylomeDB" id="Q58293"/>
    <dbReference type="BRENDA" id="2.1.1.228">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58293"/>
    <dbReference type="EvolutionaryTrace" id="Q58293"/>
    <dbReference type="PRO" id="PR:Q58293"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N(1))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009019">
        <property type="term" value="F:tRNA (guanine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="Met_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000107090" description="tRNA (guanine(37)-N1)-methyltransferase Trm5b">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <begin position="223"/>
            <end position="224"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <begin position="251"/>
            <end position="252"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 5">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="16-fold decrease in methyltransferase activity. Lack of tRNA-binding." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="20-fold decrease in methyltransferase activity. Reduced affinity for tRNA." evidence="3">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="177"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="33-fold decrease in methyltransferase activity and reduced affinity for tRNA; when associated with A-207." evidence="3">
        <original>G</original>
        <variation>A</variation>
        <location>
            <position position="205"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="33-fold decrease in methyltransferase activity and reduced affinity for tRNA; when associated with A-205." evidence="3">
        <original>G</original>
        <variation>A</variation>
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="100-fold decrease in methyltransferase activity. Lack of tRNA-binding." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="223"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="20-fold decrease in methyltransferase activity." evidence="3">
        <original>N</original>
        <variation>A</variation>
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="16-fold decrease in methyltransferase activity." evidence="3">
        <original>P</original>
        <variation>A</variation>
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="100-fold decrease in methyltransferase activity." evidence="3">
        <original>N</original>
        <variation>A</variation>
        <variation>Q</variation>
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="3-fold decrease in methyltransferase activity." evidence="3">
        <original>N</original>
        <variation>H</variation>
        <location>
            <position position="265"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="1000-fold decrease in methyltransferase activity. No change in affinity for tRNA." evidence="3">
        <original>P</original>
        <variation>A</variation>
        <location>
            <position position="267"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="27"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="32"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="37"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="51"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="59"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="77"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="85"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="91"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="98"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="12">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="131"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="turn" evidence="11">
        <location>
            <begin position="142"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="149"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="159"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="167"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="173"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="198"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="208"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="216"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="242"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="245"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="252"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="259"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="267"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="270"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="274"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="281"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="297"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="307"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15165845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16768442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="18384044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="19749755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="20980671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="2ZZM"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="2ZZN"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="2YX1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="2ZZM"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="12">
        <source>
            <dbReference type="PDB" id="3AY0"/>
        </source>
    </evidence>
    <sequence length="336" mass="39000" checksum="3E4811F0932EE95F" modified="1996-11-01" version="1">MPLCLKINKKHGEQTRRILIENNLLNKDYKITSEGNYLYLPIKDVDEDILKSILNIEFELVDKELEEKKIIKKPSFREIISKKYRKEIDEGLISLSYDVVGDLVILQISDEVDEKIRKEIGELAYKLIPCKGVFRRKSEVKGEFRVRELEHLAGENRTLTIHKENGYRLWVDIAKVYFSPRLGGERARIMKKVSLNDVVVDMFAGVGPFSIACKNAKKIYAIDINPHAIELLKKNIKLNKLEHKIIPILSDVREVDVKGNRVIMNLPKFAHKFIDKALDIVEEGGVIHYYTIGKDFDKAIKLFEKKCDCEVLEKRIVKSYAPREYILALDFKINKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58420</accession>
    <name>PSTC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable phosphate transport system permease protein PstC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">pstC</name>
        <name type="ordered locus">MJ1014</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64426">
        <property type="entry name" value="E64426"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58420"/>
    <dbReference type="STRING" id="243232.MJ_1014"/>
    <dbReference type="EnsemblBacteria" id="AAB99018">
        <property type="protein sequence ID" value="AAB99018"/>
        <property type="gene ID" value="MJ_1014"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1014"/>
    <dbReference type="eggNOG" id="arCOG00167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_1_3_2"/>
    <dbReference type="InParanoid" id="Q58420"/>
    <dbReference type="OMA" id="VIRMSVL"/>
    <dbReference type="PhylomeDB" id="Q58420"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060220" description="Probable phosphate transport system permease protein PstC">
        <location>
            <begin position="1"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="2">
        <location>
            <begin position="76"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="315" mass="35263" checksum="6003F4F6B8B68477" modified="1996-11-01" version="1">MKTMEIKKLLRKIDEFKIITLPAIFVVFILFVLILGFYFFNALPAIERYGIDLFITNVWKAAEEPAKEVYGLAAPIWGSIYTATIAVLIALPLSICYAIFVNDYAPKRLKYPLIVISDIMAGLPTIIYGIWGAFILVPLLRDHIMKFLYEHFSFIPLFDYPPLSGYCYLSAGILLGIMVTPFAAAIIREAYAMIPSVYKEGLVALGATRYETTKVLIKYIRPAIISGLILAFGRALGETVAVSLVIGNSFNLTYKLFAPGYTISSLIANQFGNAVLYEYMTSVLYSAGLVLFVIGLVVNIIGIYYLKRWREHVSH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58429</accession>
    <name>Y1023_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ1023</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1023</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64427">
        <property type="entry name" value="F64427"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870536.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58429"/>
    <dbReference type="SMR" id="Q58429"/>
    <dbReference type="STRING" id="243232.MJ_1023"/>
    <dbReference type="EnsemblBacteria" id="AAB99027">
        <property type="protein sequence ID" value="AAB99027"/>
        <property type="gene ID" value="MJ_1023"/>
    </dbReference>
    <dbReference type="GeneID" id="1451920"/>
    <dbReference type="KEGG" id="mja:MJ_1023"/>
    <dbReference type="eggNOG" id="arCOG00194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q58429"/>
    <dbReference type="OMA" id="RLICRVA"/>
    <dbReference type="OrthoDB" id="71942at2157"/>
    <dbReference type="PhylomeDB" id="Q58429"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093223" description="Uncharacterized ABC transporter ATP-binding protein MJ1023">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="5"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="262" mass="30316" checksum="B195338E6ACED777" modified="1996-11-01" version="1">MKPKIKVENLTKYFGDKKVLDEISFEVYEGEIFGLLGHNGAGKTTTLRILAGIIEEYTGYVEVNGKIGYLPEERGLYRDEKVVDVLKFFGELAGMKKEEIAKSIDYWLNKLKISNYKYSKIKELSKGNQQKVQFIVSVIHNPDIVILDEPFSGLDVVNVRLLRDIIFELKEEGKTIILSTHQLEKIERLCDRVLILKKGKAVHYGKIEDICRKMAYIEYLDNGKLIKKEIPYEEAVLILKEKAEDVIKFEVRYSLEDLFLDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58437</accession>
    <name>Y1031_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter MJ1031</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1031</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64428">
        <property type="entry name" value="F64428"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58437"/>
    <dbReference type="STRING" id="243232.MJ_1031"/>
    <dbReference type="TCDB" id="2.A.69.4.2">
        <property type="family name" value="the auxin efflux carrier (aec) family"/>
    </dbReference>
    <dbReference type="DNASU" id="1451928"/>
    <dbReference type="EnsemblBacteria" id="AAB99035">
        <property type="protein sequence ID" value="AAB99035"/>
        <property type="gene ID" value="MJ_1031"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1031"/>
    <dbReference type="eggNOG" id="arCOG04756">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056175_5_2_2"/>
    <dbReference type="InParanoid" id="Q58437"/>
    <dbReference type="OMA" id="TTFFWTL"/>
    <dbReference type="PhylomeDB" id="Q58437"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014024">
        <property type="entry name" value="Auxin_eff_plant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00946">
        <property type="entry name" value="2a69"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000123803" description="Uncharacterized transporter MJ1031">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="308" mass="33770" checksum="02A915806E4441AE" modified="1996-11-01" version="1">MIPMDVVLIVLILVLVGYFSKIFGILKEEHAKILNNIVIYIAMPSTIFLTISKNVSSSQILEFLKLPVVIFLCCLFVGILAYLLGKHIFKLKDEKLGGLILVSMLGNTGFLGYPVALGMFGEEGLARAIFCDLGGVFATMLLGTYVGIRFGKGRDKSILKDMAKFPPLITGILSIILVFFGFKLNYIPSFILKSLNYLSSATVPLIMMSLGLSLSPKALKFGVFWGIIASIFRFIVSPATAFTLSELINIKGLEKNVLLVESSMPSAMMTLVLGTLYELDIKLIASSIFITTTFSLLVIALWGWILLN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q58448</accession>
    <name>EF2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Elongation factor 2</fullName>
            <shortName>EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fusA</name>
        <name type="synonym">fus</name>
        <name type="ordered locus">MJ1048</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64430">
        <property type="entry name" value="G64430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870561.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58448"/>
    <dbReference type="SMR" id="Q58448"/>
    <dbReference type="STRING" id="243232.MJ_1048"/>
    <dbReference type="EnsemblBacteria" id="AAB99052">
        <property type="protein sequence ID" value="AAB99052"/>
        <property type="gene ID" value="MJ_1048"/>
    </dbReference>
    <dbReference type="GeneID" id="1451945"/>
    <dbReference type="KEGG" id="mja:MJ_1048"/>
    <dbReference type="eggNOG" id="arCOG01559">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002794_11_1_2"/>
    <dbReference type="InParanoid" id="Q58448"/>
    <dbReference type="OMA" id="GVMTQTE"/>
    <dbReference type="OrthoDB" id="1642at2157"/>
    <dbReference type="PhylomeDB" id="Q58448"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="SSF54980"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000091032" description="Elongation factor 2">
        <location>
            <begin position="1"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="domain" description="tr-type G">
        <location>
            <begin position="19"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="1">
        <location>
            <position position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="726" mass="80989" checksum="E5C9D63DB0971D31" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58463</accession>
    <name>Y1063_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1063</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1063</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64432">
        <property type="entry name" value="F64432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870576.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58463"/>
    <dbReference type="SMR" id="Q58463"/>
    <dbReference type="STRING" id="243232.MJ_1063"/>
    <dbReference type="DNASU" id="1451960"/>
    <dbReference type="EnsemblBacteria" id="AAB99066">
        <property type="protein sequence ID" value="AAB99066"/>
        <property type="gene ID" value="MJ_1063"/>
    </dbReference>
    <dbReference type="GeneID" id="1451960"/>
    <dbReference type="KEGG" id="mja:MJ_1063"/>
    <dbReference type="eggNOG" id="arCOG04817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072501_0_0_2"/>
    <dbReference type="InParanoid" id="Q58463"/>
    <dbReference type="OMA" id="FIIQARM"/>
    <dbReference type="OrthoDB" id="80789at2157"/>
    <dbReference type="PhylomeDB" id="Q58463"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003329">
        <property type="entry name" value="Cytidylyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02348">
        <property type="entry name" value="CTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000213212" description="Uncharacterized protein MJ1063">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <sequence length="243" mass="28442" checksum="EA93C730AD962980" modified="1996-11-01" version="1">MKIIGIIQARTGSKRLKNKVLLKLGDRCILEILLERLKKSKKLDDIIVATTIKKEDNAIVELCNSLGVNVFRGSEKDVLDRFYNASKFYSGDVIVRITGDNPLTSIELIDKQVEYLLKNNFDYVSTKNIILGLSSEVFTFDALEKAWKNAKEKYQREHVTPYIYENPNLFKVFYLEPPEYLKREGIRLTIDTIKDFKLYLELQKHFDLINVDIRQIIDFLDKNPQIKNINSNVRQKSYREVEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58474</accession>
    <name>Y1074_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1074</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1074</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the M.jannaschii MJ0023/MJ0349/MJ1072/MJ1074/MJ1107/MJECL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64434">
        <property type="entry name" value="A64434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870586.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58474"/>
    <dbReference type="SMR" id="Q58474"/>
    <dbReference type="STRING" id="243232.MJ_1074"/>
    <dbReference type="EnsemblBacteria" id="AAB99080">
        <property type="protein sequence ID" value="AAB99080"/>
        <property type="gene ID" value="MJ_1074"/>
    </dbReference>
    <dbReference type="GeneID" id="1451970"/>
    <dbReference type="KEGG" id="mja:MJ_1074"/>
    <dbReference type="eggNOG" id="arCOG09652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165881_0_0_2"/>
    <dbReference type="OMA" id="IDERFYK"/>
    <dbReference type="OrthoDB" id="123012at2157"/>
    <dbReference type="PhylomeDB" id="Q58474"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107159" description="Uncharacterized protein MJ1074">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="112" mass="13233" checksum="892E21E4BA7B24B3" modified="1997-11-01" version="1">MAIAYAKLYELIHKKIKDEREADELYNAIIEIIKESKVIVKNELKDELKDELATKKDIDLVREEMKAMEERILRYVDNRFNQLLIVQLIILFAIIITNPNAIELIKLLFGFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58483</accession>
    <name>ISF2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Iron-sulfur flavoprotein MJ1083</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Isf-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>MCJ-2</fullName>
            <shortName>Mj2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1083</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="J. Bacteriol." volume="183" first="6225" last="6233">
            <title>Iron-sulfur flavoprotein (Isf) from Methanosarcina thermophila is the prototype of a widely distributed family.</title>
            <authorList>
                <person name="Zhao T."/>
                <person name="Cruz F."/>
                <person name="Ferry J.G."/>
            </authorList>
            <dbReference type="PubMed" id="11591665"/>
            <dbReference type="DOI" id="10.1128/JB.183.21.6225-6233.2001"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF N-TERMINUS</scope>
        <scope>COFACTOR</scope>
        <scope>IRON-SULFUR CLUSTER</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="5309" last="5316">
            <title>Site-specific mutational analysis of a novel cysteine motif proposed to ligate the 4Fe-4S cluster in the iron-sulfur flavoprotein of the thermophilic methanoarchaeon Methanosarcina thermophila.</title>
            <authorList>
                <person name="Leartsakulpanich U."/>
                <person name="Antonkine M.L."/>
                <person name="Ferry J.G."/>
            </authorList>
            <dbReference type="PubMed" id="10986231"/>
            <dbReference type="DOI" id="10.1128/JB.182.19.5309-5316.2000"/>
        </citation>
        <scope>PROTEIN FAMILY</scope>
    </reference>
    <comment type="function">
        <text>Redox-active protein probably involved in electron transport.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="2">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="subunit">
        <text>Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SsuE family. Isf subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64435">
        <property type="entry name" value="B64435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870595.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58483"/>
    <dbReference type="SMR" id="Q58483"/>
    <dbReference type="STRING" id="243232.MJ_1083"/>
    <dbReference type="EnsemblBacteria" id="AAB99085">
        <property type="protein sequence ID" value="AAB99085"/>
        <property type="gene ID" value="MJ_1083"/>
    </dbReference>
    <dbReference type="GeneID" id="1451979"/>
    <dbReference type="KEGG" id="mja:MJ_1083"/>
    <dbReference type="eggNOG" id="arCOG02573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050993_3_0_2"/>
    <dbReference type="InParanoid" id="Q58483"/>
    <dbReference type="OMA" id="KFADGNP"/>
    <dbReference type="OrthoDB" id="89591at2157"/>
    <dbReference type="PhylomeDB" id="Q58483"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="SSF52218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000160600" description="Iron-sulfur flavoprotein MJ1083">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11591665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="11591665"/>
        </source>
    </evidence>
    <sequence length="193" mass="20648" checksum="EB58694A037D2F0B" modified="1996-11-01" version="1">MKVIGISGSPRPEGNTTLLVREALNAIAEEGIETEFISLADKELNPCIGCNMCKEEGKCPIIDDVDEILKKMKEADGIILGSPVYFGGVSAQLKMLMDRSRPLRIGFQLRNKVGGAVAVGASRNGGQETTIQQIHNFFLIHSMIVVGDNDPTAHYGGTGVGKAPGDCKNDDIGLETARNLGKKVAEVVKLIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="149" xmlns="http://uniprot.org/uniprot">
    <accession>Q58487</accession>
    <name>MVK_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Mevalonate kinase</fullName>
            <shortName evidence="1">MK</shortName>
            <shortName evidence="1">MVK</shortName>
            <ecNumber evidence="1">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mvk</name>
        <name type="ordered locus">MJ1087</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="Protein Expr. Purif." volume="17" first="33" last="40">
            <title>Overexpression, purification, and characterization of the thermostable mevalonate kinase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Huang K.-X."/>
                <person name="Scott A.I."/>
                <person name="Bennett G.N."/>
            </authorList>
            <dbReference type="PubMed" id="10497066"/>
            <dbReference type="DOI" id="10.1006/prep.1999.1106"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Protein Expr. Purif." volume="30" first="210" last="218">
            <title>Expression and purification of Arg196 and Lys272 mutants of mevalonate kinase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Chu X."/>
                <person name="Liu X."/>
                <person name="Yau M."/>
                <person name="Leung Y.C."/>
                <person name="Li D."/>
            </authorList>
            <dbReference type="PubMed" id="12880770"/>
            <dbReference type="DOI" id="10.1016/S1046-5928(03)00101-3"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>MUTAGENESIS OF ARG-196 AND LYS-272</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="9462" last="9467">
            <title>Structure of the Methanococcus jannaschii mevalonate kinase, a member of the GHMP kinase superfamily.</title>
            <authorList>
                <person name="Yang D."/>
                <person name="Shipman L.W."/>
                <person name="Roessner C.A."/>
                <person name="Scott A.I."/>
                <person name="Sacchettini J.C."/>
            </authorList>
            <dbReference type="PubMed" id="11751891"/>
            <dbReference type="DOI" id="10.1074/jbc.M110787200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS)</scope>
        <scope>ACTIVE SITE</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2005" name="Proteins" volume="60" first="787" last="796">
            <title>Structural analysis of a set of proteins resulting from a bacterial genomics project.</title>
            <authorList>
                <person name="Badger J."/>
                <person name="Sauder J.M."/>
                <person name="Adams J.M."/>
                <person name="Antonysamy S."/>
                <person name="Bain K."/>
                <person name="Bergseid M.G."/>
                <person name="Buchanan S.G."/>
                <person name="Buchanan M.D."/>
                <person name="Batiyenko Y."/>
                <person name="Christopher J.A."/>
                <person name="Emtage S."/>
                <person name="Eroshkina A."/>
                <person name="Feil I."/>
                <person name="Furlong E.B."/>
                <person name="Gajiwala K.S."/>
                <person name="Gao X."/>
                <person name="He D."/>
                <person name="Hendle J."/>
                <person name="Huber A."/>
                <person name="Hoda K."/>
                <person name="Kearins P."/>
                <person name="Kissinger C."/>
                <person name="Laubert B."/>
                <person name="Lewis H.A."/>
                <person name="Lin J."/>
                <person name="Loomis K."/>
                <person name="Lorimer D."/>
                <person name="Louie G."/>
                <person name="Maletic M."/>
                <person name="Marsh C.D."/>
                <person name="Miller I."/>
                <person name="Molinari J."/>
                <person name="Muller-Dieckmann H.J."/>
                <person name="Newman J.M."/>
                <person name="Noland B.W."/>
                <person name="Pagarigan B."/>
                <person name="Park F."/>
                <person name="Peat T.S."/>
                <person name="Post K.W."/>
                <person name="Radojicic S."/>
                <person name="Ramos A."/>
                <person name="Romero R."/>
                <person name="Rutter M.E."/>
                <person name="Sanderson W.E."/>
                <person name="Schwinn K.D."/>
                <person name="Tresser J."/>
                <person name="Winhoven J."/>
                <person name="Wright T.A."/>
                <person name="Wu L."/>
                <person name="Xu J."/>
                <person name="Harris T.J."/>
            </authorList>
            <dbReference type="PubMed" id="16021622"/>
            <dbReference type="DOI" id="10.1002/prot.20541"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.69 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Farnesyl- and geranyl-pyrophosphates are competitive inhibitors. Slightly inhibited by high concentration of ATP.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2 3">68.5 uM for (RS)-mevalonate (at 70 degrees Celsius)</KM>
            <KM evidence="2 3">106 uM for (RS)-mevalonate (at 34 degrees Celsius)</KM>
            <KM evidence="2 3">92 uM for ATP (at 70 degrees Celsius)</KM>
            <KM evidence="2 3">1180 uM for ATP (at 34 degrees Celsius)</KM>
            <Vmax evidence="2 3">387.0 umol/min/mg enzyme (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2 3">50.3 umol/min/mg enzyme (at 34 degrees Celsius)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 8.0-8.5. Exhibits at least 60% of its optimal activity over a rather broad pH range, from 5 to 7.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 70-75 degrees Celsius. Highly thermostable. Retains 100% of its activity after 24 hours of incubation at 70 degrees Celsius. At 90 and 100 degrees Celsius, the enzyme shows a half-life of 15 and 5 min, respectively.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64435">
        <property type="entry name" value="F64435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870599.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1KKH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=1-312"/>
    </dbReference>
    <dbReference type="PDB" id="1VIS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.69 A"/>
        <property type="chains" value="A=2-312"/>
    </dbReference>
    <dbReference type="PDBsum" id="1KKH"/>
    <dbReference type="PDBsum" id="1VIS"/>
    <dbReference type="AlphaFoldDB" id="Q58487"/>
    <dbReference type="SMR" id="Q58487"/>
    <dbReference type="STRING" id="243232.MJ_1087"/>
    <dbReference type="EnsemblBacteria" id="AAB99088">
        <property type="protein sequence ID" value="AAB99088"/>
        <property type="gene ID" value="MJ_1087"/>
    </dbReference>
    <dbReference type="GeneID" id="1451983"/>
    <dbReference type="KEGG" id="mja:MJ_1087"/>
    <dbReference type="eggNOG" id="arCOG01028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017814_0_0_2"/>
    <dbReference type="InParanoid" id="Q58487"/>
    <dbReference type="OMA" id="NTVCTYG"/>
    <dbReference type="OrthoDB" id="90882at2157"/>
    <dbReference type="PhylomeDB" id="Q58487"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14622"/>
    <dbReference type="BRENDA" id="2.7.1.36">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58487"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58487"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="PTHR43290"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156665" description="Mevalonate kinase">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="13.5-fold decrease in affinity for mevalonate, whereas only little effect on affinity for ATP and on reaction rate." evidence="3">
        <original>R</original>
        <variation>K</variation>
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="1900-fold decrease in catalytic efficiency." evidence="3">
        <original>R</original>
        <variation>Q</variation>
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="63-fold decrease in catalytic efficiency." evidence="3">
        <original>R</original>
        <variation>V</variation>
        <location>
            <position position="196"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="13- to 26-fold decrease in catalytic efficiency. Still thermostable." evidence="3">
        <original>K</original>
        <variation>R</variation>
        <variation>A</variation>
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="71"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="95"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="105"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="111"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="154"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="163"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="172"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="177"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="188"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="200"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="233"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="249"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="268"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="275"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="288"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="291"/>
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        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="304"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10497066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12880770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="11751891"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1KKH"/>
        </source>
    </evidence>
    <sequence length="312" mass="35177" checksum="DFF9E5B869728298" modified="1996-11-01" version="1">MIIETPSKVILFGEHAVVYGYRAISMAIDLTSTIEIKETQEDEIILNLNDLNKSLGLNLNEIKNINPNNFGDFKYCLCAIKNTLDYLNIEPKTGFKINISSKIPISCGLGSSASITIGTIKAVSGFYNKELKDDEIAKLGYMVEKEIQGKASITDTSTITYKGILEIKNNKFRKIKGEFEEFLKNCKFLIVYAEKRKKKTAELVNEVAKIENKDEIFKEIDKVIDEALKIKNKEDFGKLMTKNHELLKKLNISTPKLDRIVDIGNRFGFGAKLTGAGGGGCVIILVNEEKEKELLKELNKEDVRIFNCRMMN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58489</accession>
    <name>Y1089_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1089</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1089</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CbiQ family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64435">
        <property type="entry name" value="H64435"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58489"/>
    <dbReference type="SMR" id="Q58489"/>
    <dbReference type="STRING" id="243232.MJ_1089"/>
    <dbReference type="EnsemblBacteria" id="AAB99090">
        <property type="protein sequence ID" value="AAB99090"/>
        <property type="gene ID" value="MJ_1089"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1089"/>
    <dbReference type="eggNOG" id="arCOG02249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_5_0_2"/>
    <dbReference type="InParanoid" id="Q58489"/>
    <dbReference type="OMA" id="IAMMMYR"/>
    <dbReference type="PhylomeDB" id="Q58489"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107165" description="Uncharacterized protein MJ1089">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="237"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="268" mass="30904" checksum="B9483380C8EC325E" modified="1996-11-01" version="1">MGFMKHNIVDNVAFSNKLRHVNPKLKVIFALSLLLISVFSTSFIVPLIIFFINSILLLFKAKVPKKIYAVFVGIPLGFGILNLVIFAFLFGTVEWFKINVFGFEIPVYKDGIELGLLLFGRMLGGVSSMLFLAFTTPMVELFYIFRELKMPDVLVDMMMLIYRYIFVLYEEYEKMKFAQESRLGTSNLKSTYKSLGALAAHLFIRAWEKGEKLNITMMSRCYDGKIKLLQTIENPSIKYILFIAIFDIFLIILAYLTKDFTLTSYIKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58506</accession>
    <name>Y1106_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1106</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1106</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH236.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64438">
        <property type="entry name" value="A64438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870618.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58506"/>
    <dbReference type="SMR" id="Q58506"/>
    <dbReference type="STRING" id="243232.MJ_1106"/>
    <dbReference type="EnsemblBacteria" id="AAB99109">
        <property type="protein sequence ID" value="AAB99109"/>
        <property type="gene ID" value="MJ_1106"/>
    </dbReference>
    <dbReference type="GeneID" id="1452003"/>
    <dbReference type="KEGG" id="mja:MJ_1106"/>
    <dbReference type="eggNOG" id="arCOG04822">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129114_0_0_2"/>
    <dbReference type="OMA" id="PECCIKS"/>
    <dbReference type="OrthoDB" id="90313at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007556">
        <property type="entry name" value="DUF483"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04467">
        <property type="entry name" value="DUF483"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107169" description="Uncharacterized protein MJ1106">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="185" mass="22110" checksum="E991917F84DC6E36" modified="1996-11-01" version="1">MLKNLLYKIEKLRSGELEGFEVLKEHIQSLDEFQYQQIVERLKFQIELVEKYKPKVRPAIDPMVSTELGIYRRLDDFEIGKLLDYPECCIKSFVEDVRVAIDREHLKEVEEMKEELKNKGIYAIVLPSGFIPCSLKCEEAIKRGFIGYLTKEEFDKILELEKELKEKIRHWHFGYDEYYEKIILP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58532</accession>
    <name>Y1132_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0058 protein MJ1132</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1132</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0058 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64441">
        <property type="entry name" value="C64441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870643.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58532"/>
    <dbReference type="SMR" id="Q58532"/>
    <dbReference type="STRING" id="243232.MJ_1132"/>
    <dbReference type="EnsemblBacteria" id="AAB99134">
        <property type="protein sequence ID" value="AAB99134"/>
        <property type="gene ID" value="MJ_1132"/>
    </dbReference>
    <dbReference type="GeneID" id="1452028"/>
    <dbReference type="KEGG" id="mja:MJ_1132"/>
    <dbReference type="eggNOG" id="arCOG02254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_167318_0_0_2"/>
    <dbReference type="OMA" id="PHHIHRL"/>
    <dbReference type="OrthoDB" id="121332at2157"/>
    <dbReference type="PhylomeDB" id="Q58532"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="PTHR42203"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="SSF140371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135709" description="UPF0058 protein MJ1132">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="118" mass="13841" checksum="EF55ED33865142B1" modified="1996-11-01" version="1">MHKEQLMKLHQFFVYVVKEIMDDNLENTDNECKKLFKIYEMLDIRPHHIHRLKSEQKAAILLLSACVASYLANNMDNVPKNLAKKLEENAFKHLNSCKKNIIILEENENNGESAEKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58534</accession>
    <name>Y1134_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1134</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1134</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64441">
        <property type="entry name" value="E64441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870645.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58534"/>
    <dbReference type="SMR" id="Q58534"/>
    <dbReference type="STRING" id="243232.MJ_1134"/>
    <dbReference type="EnsemblBacteria" id="AAB99136">
        <property type="protein sequence ID" value="AAB99136"/>
        <property type="gene ID" value="MJ_1134"/>
    </dbReference>
    <dbReference type="GeneID" id="1452030"/>
    <dbReference type="KEGG" id="mja:MJ_1134"/>
    <dbReference type="eggNOG" id="arCOG02610">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_070251_1_1_2"/>
    <dbReference type="InParanoid" id="Q58534"/>
    <dbReference type="OMA" id="GKVDPWN"/>
    <dbReference type="OrthoDB" id="81037at2157"/>
    <dbReference type="PhylomeDB" id="Q58534"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003768">
        <property type="entry name" value="ScpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33969">
        <property type="entry name" value="PTHR33969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02616">
        <property type="entry name" value="SMC_ScpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107182" description="Uncharacterized protein MJ1134">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="121"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="226" mass="26892" checksum="12AFAF1A3DAE32EF" modified="1996-11-01" version="1">MIDSNFDIVLWVRMIKEGIEKKNLNPWDVNIAEIADYYIQKIKELKKFDIRLSADVILVAGILLRMKSEALYDECKVEEEEDYDYCDDYYDYDDIEEKPKKGKKKEKEDKDKNKKSKKPVTVDELIKTIEKELNKVKKSRKNREKKTNEVEEIIEELIEEDDISDIIAELLDDLMKEGIIVYQEKFKTREDRVRYFIPSLYLANDGKAELIQEKLFGELIIKLKSF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58547</accession>
    <name>Y1147_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1147</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1147</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64443">
        <property type="entry name" value="B64443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870658.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58547"/>
    <dbReference type="STRING" id="243232.MJ_1147"/>
    <dbReference type="PRIDE" id="Q58547"/>
    <dbReference type="EnsemblBacteria" id="AAB99156">
        <property type="protein sequence ID" value="AAB99156"/>
        <property type="gene ID" value="MJ_1147"/>
    </dbReference>
    <dbReference type="GeneID" id="1452043"/>
    <dbReference type="KEGG" id="mja:MJ_1147"/>
    <dbReference type="eggNOG" id="arCOG03128">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037290_0_0_2"/>
    <dbReference type="OMA" id="LYKMEME"/>
    <dbReference type="OrthoDB" id="17316at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001646">
        <property type="entry name" value="5peptide_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13576">
        <property type="entry name" value="Pentapeptide_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107188" description="Uncharacterized protein MJ1147">
        <location>
            <begin position="1"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="462" mass="54433" checksum="91FF068862971776" modified="1996-11-01" version="1">MFILIIKCGIMEKEVISSREFIDRFVECLEKGEDFKLKDCVVEGNVDILNIYEMIKDKELKGGYIEKKDDEIVVNINIKVDIYNVEFNGDFRFFVNMEYQIVISVFNGNAYFRVITFKGSVYFIRTIFNGDVDFIDTIFEENAYFSVTAFKGNIINFSGTIFNKESHFKSTTFEGNTYFSVTTFNIAEFYNSTFKSHVYFDDISFNLLSFTDCRFRDDVSFKKIDKENFKGLAIFLKTQFLNKHTTIENFQLSKTSFLKTDVREVLLCDVKKEEILSHKILRIKEDSGNKDKDLENKLKELLGLSYKYIIDQFNYKSVLAEYRNLRISIENNRTYIEASNLYKMEMELIKEFSNGRFEKFIIGAYGAISDYGESMEKTGKWILGSMILFTILASILRFKGMEWDIFKIIEFWWISFWEVIRLFLQIGTEDKSLWILEPIIRVTSLILLGNLYIAVRRKLSRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58571</accession>
    <name>FWDC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C</fullName>
            <ecNumber evidence="2">1.2.7.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Tungsten-containing formylmethanofuran dehydrogenase II subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">fwdC</name>
        <name type="ordered locus">MJ1171</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">This enzyme is composed of six subunits FwdA, FwdC, FwdD, FwdE, FwdF and FwdG.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FwdC/FmdC family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64446">
        <property type="entry name" value="B64446"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870684.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58571"/>
    <dbReference type="SMR" id="Q58571"/>
    <dbReference type="STRING" id="243232.MJ_1171"/>
    <dbReference type="EnsemblBacteria" id="AAB99173">
        <property type="protein sequence ID" value="AAB99173"/>
        <property type="gene ID" value="MJ_1171"/>
    </dbReference>
    <dbReference type="GeneID" id="1452069"/>
    <dbReference type="KEGG" id="mja:MJ_1171"/>
    <dbReference type="eggNOG" id="arCOG00097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072248_0_0_2"/>
    <dbReference type="InParanoid" id="Q58571"/>
    <dbReference type="OMA" id="VNGDAGM"/>
    <dbReference type="OrthoDB" id="72252at2157"/>
    <dbReference type="PhylomeDB" id="Q58571"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00980">
        <property type="entry name" value="FwdC/FmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017550">
        <property type="entry name" value="Formylmethanofuran_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03122">
        <property type="entry name" value="one_C_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000144194" description="Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="97"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="repeat" description="3">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="repeat" description="4">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="repeat" description="5">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="repeat" description="6">
        <location>
            <begin position="180"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="repeat" description="7">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="7 X 13 AA repeats of [GW]-X-X-[MQ]-X-X-G-X-[IL]-X-[IV]-X-G">
        <location>
            <begin position="78"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q48943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="273" mass="29461" checksum="B34219D170D9C1AD" modified="1996-11-01" version="1">MKELILTLQKEIIVPVEMDKVLPEVIENMSLEEIKNIELVQGRKRIKVADIFDVELNDIEGEPRIVIKNSSPKLKYIGSKMTKGEIVVEGDAGMYVGAEMKGGKIVVNGNAESWAGQNMKGGELLIKGNAGDYVGSAYRGDWRGMSGGTIIVEGNAGNEIGEFMSKGLIHIKGNVGIMAGIHQNGGIIIIDGDVDVRVGGEMKAGAIVVYGKVEEILPSFKFEGIVENPVIKLSKKDAGTPIAGTFYKFSGDYVYNKPKGQLYISVDSNPDLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58621</accession>
    <name>Y1224_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0216 protein MJ1224</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1224</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0216 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64452">
        <property type="entry name" value="G64452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870736.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58621"/>
    <dbReference type="SMR" id="Q58621"/>
    <dbReference type="STRING" id="243232.MJ_1224"/>
    <dbReference type="EnsemblBacteria" id="AAB99227">
        <property type="protein sequence ID" value="AAB99227"/>
        <property type="gene ID" value="MJ_1224"/>
    </dbReference>
    <dbReference type="GeneID" id="1452120"/>
    <dbReference type="KEGG" id="mja:MJ_1224"/>
    <dbReference type="eggNOG" id="arCOG01921">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146474_1_0_2"/>
    <dbReference type="InParanoid" id="Q58621"/>
    <dbReference type="OMA" id="LHNLKSN"/>
    <dbReference type="OrthoDB" id="112654at2157"/>
    <dbReference type="PhylomeDB" id="Q58621"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00585">
        <property type="entry name" value="UPF0216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002746">
        <property type="entry name" value="UPF0216"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01886">
        <property type="entry name" value="DUF61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005264">
        <property type="entry name" value="UCP005264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000144224" description="UPF0216 protein MJ1224">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00585"/>
        </source>
    </evidence>
    <sequence length="139" mass="16202" checksum="6FB6D0CCC31F00E3" modified="1996-11-01" version="1">MIILVMSMRDVEKIIKGIIKDMNPRFKRKTLRELLSEEKPHVIINGKRHRIKRRELEFLKEIASEDLKIPIVLEVDSSLGGAIKISGKEEVKVISKILGKEIDIFSEKDVMYIYKPELKIVRKELPTTTQLIFKLSLFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="144" xmlns="http://uniprot.org/uniprot">
    <accession>Q58635</accession>
    <name>SYP_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Proline--tRNA ligase</fullName>
            <ecNumber>6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Prolyl-tRNA synthetase</fullName>
            <shortName>ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">proS</name>
        <name type="ordered locus">MJ1238</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Science" volume="287" first="479" last="482">
            <title>One polypeptide with two aminoacyl-tRNA synthetase activities.</title>
            <authorList>
                <person name="Stathopoulos C."/>
                <person name="Li T."/>
                <person name="Longman R."/>
                <person name="Vothknecht U.C."/>
                <person name="Becker H.D."/>
                <person name="Ibba M."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="10642548"/>
            <dbReference type="DOI" id="10.1126/science.287.5452.479"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-18</scope>
        <scope>FUNCTION AS A PROLYL-TRNA SYNTHETASE</scope>
        <scope>PUTATIVE FUNCTION AS A CYSTEINYL-TRNA SYNTHETASE</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="Biochemistry" volume="39" first="7792" last="7798">
            <title>Synthesis of cysteinyl-tRNA(Cys) by a genome that lacks the normal cysteine-tRNA synthetase.</title>
            <authorList>
                <person name="Lipman R.S.A."/>
                <person name="Sowers K.R."/>
                <person name="Hou Y.-M."/>
            </authorList>
            <dbReference type="PubMed" id="10869184"/>
            <dbReference type="DOI" id="10.1021/bi0004955"/>
        </citation>
        <scope>FUNCTION AS A PROLYL-TRNA SYNTHETASE</scope>
        <scope>PUTATIVE FUNCTION AS A CYSTEINYL-TRNA SYNTHETASE</scope>
        <scope>INHIBITION BY PROLINAMIDE</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2001" name="Biochemistry" volume="40" first="46" last="52">
            <title>Methanococcus jannaschii prolyl-cysteinyl-tRNA synthetase possesses overlapping amino acid binding sites.</title>
            <authorList>
                <person name="Stathopoulos C."/>
                <person name="Jacquin-Becker C."/>
                <person name="Becker H.D."/>
                <person name="Li T."/>
                <person name="Ambrogelly A."/>
                <person name="Longman R."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="11141055"/>
            <dbReference type="DOI" id="10.1021/bi002108x"/>
        </citation>
        <scope>FUNCTION AS A PROLYL-TRNA SYNTHETASE</scope>
        <scope>PUTATIVE FUNCTION AS A CYSTEINYL-TRNA SYNTHETASE</scope>
        <scope>KINETIC PARAMETERS</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2001" name="J. Biol. Chem." volume="276" first="30779" last="30785">
            <title>Species-specific differences in amino acid editing by class II prolyl-tRNA synthetase.</title>
            <authorList>
                <person name="Beuning P.J."/>
                <person name="Musier-Forsyth K."/>
            </authorList>
            <dbReference type="PubMed" id="11408489"/>
            <dbReference type="DOI" id="10.1074/jbc.M104761200"/>
        </citation>
        <scope>MISAMINOACYLATION OF TRNA(PRO) WITH ALANINE OR CYSTEINE</scope>
        <scope>EDITING ACTIVITY AGAINST ALANINE</scope>
        <scope>KINETIC PARAMETERS</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="34743" last="34748">
            <title>Cysteine activation is an inherent in vitro property of prolyl-tRNA synthetases.</title>
            <authorList>
                <person name="Ahel I."/>
                <person name="Stathopoulos C."/>
                <person name="Ambrogelly A."/>
                <person name="Sauerwald A."/>
                <person name="Toogood H."/>
                <person name="Hartsch T."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="12130657"/>
            <dbReference type="DOI" id="10.1074/jbc.M206928200"/>
        </citation>
        <scope>MISAMINOACYLATION OF TRNA(PRO) WITH CYSTEINE</scope>
        <scope>KINETIC PARAMETERS</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="34749" last="34754">
            <title>Methanocaldococcus jannaschii prolyl-tRNA synthetase charges tRNA(Pro) with cysteine.</title>
            <authorList>
                <person name="Ambrogelly A."/>
                <person name="Ahel I."/>
                <person name="Polycarpo C."/>
                <person name="Bunjun-Srihari S."/>
                <person name="Krett B."/>
                <person name="Jacquin-Becker C."/>
                <person name="Ruan B."/>
                <person name="Koehrer C."/>
                <person name="Stathopoulos C."/>
                <person name="RajBhandary U.L."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="12130658"/>
            <dbReference type="DOI" id="10.1074/jbc.M206929200"/>
        </citation>
        <scope>MISAMINOACYLATION OF TRNA(PRO) WITH CYSTEINE</scope>
        <scope>INABILITY TO SYNTHESIZE CYSTEINYL-TRNA(CYS)</scope>
        <scope>LACK OF EDITING ACTIVITY</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2002" name="J. Mol. Biol." volume="316" first="421" last="427">
            <title>Amino acid activation of a dual-specificity tRNA synthetase is independent of tRNA.</title>
            <authorList>
                <person name="Lipman R.S.A."/>
                <person name="Beuning P.J."/>
                <person name="Musier-Forsyth K."/>
                <person name="Hou Y.-M."/>
            </authorList>
            <dbReference type="PubMed" id="11866507"/>
            <dbReference type="DOI" id="10.1006/jmbi.2001.5373"/>
        </citation>
        <scope>FUNCTION IN CYSTEINE ACTIVATION</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2004" name="J. Bacteriol." volume="186" first="8" last="14">
            <title>Cysteinyl-tRNA(Cys) formation in Methanocaldococcus jannaschii: the mechanism is still unknown.</title>
            <authorList>
                <person name="Ruan B."/>
                <person name="Nakano H."/>
                <person name="Tanaka M."/>
                <person name="Mills J.A."/>
                <person name="DeVito J.A."/>
                <person name="Min B."/>
                <person name="Low K.B."/>
                <person name="Battista J.R."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="14679218"/>
            <dbReference type="DOI" id="10.1128/JB.186.1.8-14.2004"/>
        </citation>
        <scope>INABILITY TO SYNTHESIZE CYSTEINYL-TRNA(CYS)</scope>
    </reference>
    <reference key="10">
        <citation type="journal article" date="2005" name="FEBS Lett." volume="579" first="6017" last="6022">
            <title>Asymmetric behavior of archaeal prolyl-tRNA synthetase.</title>
            <authorList>
                <person name="Ambrogelly A."/>
                <person name="Kamtekar S."/>
                <person name="Stathopoulos C."/>
                <person name="Kennedy D."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="16226256"/>
            <dbReference type="DOI" id="10.1016/j.febslet.2005.09.025"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="11">
        <citation type="journal article" date="2003" name="Proc. Natl. Acad. Sci. U.S.A." volume="100" first="1673" last="1678">
            <title>The structural basis of cysteine aminoacylation of tRNAPro by prolyl-tRNA synthetases.</title>
            <authorList>
                <person name="Kamtekar S."/>
                <person name="Kennedy W.D."/>
                <person name="Wang J."/>
                <person name="Stathopoulos C."/>
                <person name="Soell D."/>
                <person name="Steitz T.A."/>
            </authorList>
            <dbReference type="PubMed" id="12578991"/>
            <dbReference type="DOI" id="10.1073/pnas.0437911100"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF APOENZYME</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 4 6">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). Can inadvertently accommodate and process non-cognate amino acids such as cysteine and alanine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text>Inhibited by high concentrations of prolinamide.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="4 5 7">0.28 mM for proline (at 60 degrees Celsius)</KM>
            <KM evidence="4 5 7">0.09 mM for cysteine (at 60 degrees Celsius)</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Homodimer. The dimer is functionally asymmetric: only one of the two active sites at a time is able to form prolyl-adenylate, and only one tRNA molecule binds per dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Was originally (PubMed:10642548, PubMed:10869184 and PubMed:11141055) thought to have both prolyl- and cysteinyl-tRNA synthetase activities (ProCysRS). However, recent biochemical and structural studies show that ProRS misaminoacylates tRNA(Pro) with cysteine but is unable to aminoacylate tRNA(Cys). These conflicting results may be due to the fact that much of the previous work was done with unfractionated tRNA.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">According to PubMed:12130658, ProRS is unable to edit the misacylated Cys-tRNA(Pro) and Ala-tRNA(Pro), whereas according to PubMed:11408489, it hydrolyzes Ala-AMP and Ala-tRNA(Pro) by 'pretransfer' and 'posttransfer' editing activities, respectively.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64454">
        <property type="entry name" value="E64454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870750.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1NJ8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="A/B/C/D=2-455"/>
    </dbReference>
    <dbReference type="PDBsum" id="1NJ8"/>
    <dbReference type="AlphaFoldDB" id="Q58635"/>
    <dbReference type="SMR" id="Q58635"/>
    <dbReference type="STRING" id="243232.MJ_1238"/>
    <dbReference type="EnsemblBacteria" id="AAB99242">
        <property type="protein sequence ID" value="AAB99242"/>
        <property type="gene ID" value="MJ_1238"/>
    </dbReference>
    <dbReference type="GeneID" id="1452134"/>
    <dbReference type="KEGG" id="mja:MJ_1238"/>
    <dbReference type="eggNOG" id="arCOG00402">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001882_4_2_2"/>
    <dbReference type="InParanoid" id="Q58635"/>
    <dbReference type="OMA" id="EVYWVTH"/>
    <dbReference type="OrthoDB" id="14454at2157"/>
    <dbReference type="PhylomeDB" id="Q58635"/>
    <dbReference type="BRENDA" id="6.1.1.15">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58635"/>
    <dbReference type="EvolutionaryTrace" id="Q58635"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    <sequence length="455" mass="53309" checksum="3D155A36AFE0CC3B" modified="1996-11-01" version="1">MEFSEWYSDILEKAEIYDVRYPIKGCGVYLPYGFKIRRYTFEIIRNLLDESGHDEALFPMLIPEDLLAKEAEHIKGFEDEVYWVTHGGKTQLDVKLALRPTSETPIYYMMKLWVKVHTDLPIKIYQIVNTFRYETKHTRPLIRLREIMTFKEAHTAHSTKEEAENQVKEAISIYKKFFDTLGIPYLISKRPEWDKFPGAEYTMAFDTIFPDGRTMQIATVHNLGQNFSKTFEIIFETPTGDKDYAYQTCYGISDRVIASIIAIHGDEKGLILPPIVAPIQVVIVPLIFKGKEDIVMEKAKEIYEKLKGKFRVHIDDRDIRPGRKFNDWEIKGVPLRIEVGPKDIENKKITLFRRDTMEKFQVDETQLMEVVEKTLNNIMENIKNRAWEKFENFITILEDINPDEIKNILSEKRGVILVPFKEEIYNEELEEKVEATILGETEYKGNKYIAIAKTY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58650</accession>
    <name>SECF_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secF</name>
        <name type="ordered locus">MJ1253</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the protein translocation apparatus. Forms a complex with SecD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D64456"/>
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    </dbReference>
    <dbReference type="GeneID" id="1452151"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_060478_0_0_2"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:intracellular protein transmembrane transport"/>
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    </dbReference>
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        <property type="entry name" value="SecF_A"/>
        <property type="match status" value="1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000095991" description="Protein-export membrane protein SecF">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <sequence length="282" mass="31029" checksum="D2AC5859AEFC1079" modified="1996-11-01" version="1">MIKDYKVSIAIPIALLILSILLIGFKGIPKSIDITGGTEITIKVNENMDITPLKESLNGIAEVKKLESADGYYIVIRCKNEDVDIVKQKIKEFFHVDSLDKLNYSEKTIGATLSSKFFEEGFKAVGFAFMFMAIVVYLYFRNPVPSGAIILSALSDIIMALGAMSLLGIELSSATIAALLMVIGYSVDSDILLTTRVLKRLTKSFDETVKEAMKTGLTMTLTTITAMLILLIVVKLFIPVADILANIATVLILALIADIINTWLLNAGILKYYITEYRAKKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58689</accession>
    <name>Y1293_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1293</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1293</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64461">
        <property type="entry name" value="D64461"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58689"/>
    <dbReference type="SMR" id="Q58689"/>
    <dbReference type="STRING" id="243232.MJ_1293"/>
    <dbReference type="EnsemblBacteria" id="AAB99309">
        <property type="protein sequence ID" value="AAB99309"/>
        <property type="gene ID" value="MJ_1293"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1293"/>
    <dbReference type="eggNOG" id="arCOG05057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197645_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107260" description="Uncharacterized protein MJ1293">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="231" mass="26686" checksum="D7D34948088BC95A" modified="1996-11-01" version="1">MTNKTRKETNEIKNNITLPPNIKTEVNRTSKYDFTNLSKDLEIVYALIKITGFDEYLPFNIENLNKIFIKEKISPYPYLLNLIKDLIILFVIGLIITIIGLLMYEPTRPKVISIIASILYKLKIKEKPKPKKKETIKLPKPPKIYISDVIYSLGDTVRIEISSEIDIGNNLYILSPTNKKYKIELIKTGKNKYLGLFKIPENEVPGQYFIIYKPENLSIGGFLVVDIKKEM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58696</accession>
    <name>BIOA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
            <ecNumber evidence="1">2.6.1.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7,8-diamino-pelargonic acid aminotransferase</fullName>
            <shortName evidence="1">DAPA AT</shortName>
            <shortName evidence="1">DAPA aminotransferase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">7,8-diaminononanoate synthase</fullName>
            <shortName evidence="1">DANS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Diaminopelargonic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioA</name>
        <name type="ordered locus">MJ1300</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate</text>
            <dbReference type="Rhea" id="RHEA:16861"/>
            <dbReference type="ChEBI" id="CHEBI:16490"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="EC" id="2.6.1.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99307" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.62" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99307.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64462">
        <property type="entry name" value="C64462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496781.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6ZHK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-461"/>
    </dbReference>
    <dbReference type="PDBsum" id="6ZHK"/>
    <dbReference type="AlphaFoldDB" id="Q58696"/>
    <dbReference type="SMR" id="Q58696"/>
    <dbReference type="STRING" id="243232.MJ_1300"/>
    <dbReference type="EnsemblBacteria" id="AAB99307">
        <property type="protein sequence ID" value="AAB99307"/>
        <property type="gene ID" value="MJ_1300"/>
    </dbReference>
    <dbReference type="GeneID" id="1452202"/>
    <dbReference type="KEGG" id="mja:MJ_1300"/>
    <dbReference type="eggNOG" id="arCOG00917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_4_3_2"/>
    <dbReference type="InParanoid" id="Q58696"/>
    <dbReference type="OrthoDB" id="20774at2157"/>
    <dbReference type="PhylomeDB" id="Q58696"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00160"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00834">
        <property type="entry name" value="BioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005815">
        <property type="entry name" value="BioA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00508">
        <property type="entry name" value="bioA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000120377" description="Adenosylmethionine-8-amino-7-oxononanoate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="331"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM" evidence="1">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="5"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="23"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="33"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="41"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="49"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="55"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="110"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="117"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="142"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="155"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="164"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="168"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="178"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="220"/>
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    <feature type="helix" evidence="3">
        <location>
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    <feature type="helix" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="361"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="372"/>
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    </feature>
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        <location>
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    <feature type="strand" evidence="3">
        <location>
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    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="398"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="406"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="431"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="442"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="6ZHK"/>
        </source>
    </evidence>
    <sequence length="461" mass="52314" checksum="8CF29B3FCBC280FC" modified="2009-09-22" version="2">MNIDKNLLEKWDKEYIWHPYTQMKEYRESKNLIIERGEGNYLIDIYGNKYLDAVSSIWCNLFGHSRKEIIEAIKNQADKICHSTLLGCGNVPSILLAKKLVDITPKHLTKVFYSEDGAEAVEIAIKMAYQYYVLRGDKGRTKFISVKEGYHGDTVGAMSVGGSELFHGVFKPLLFKGYHANPPYCYRCKYHNFKDTDERNEKGCEMECLNEMISLIEKHAEEVFCVILEGGIMGSAGMIPYPDGYIEGVAKACKENDVIFILDEVATGFGRTGKMFFCDNEELKKLEKPDILCLGKGLTGGYLPLAATLTTDEIYNQFLGEFGESKQLYHGHTYTGNQLLCSAALATLEIFEKENVIENIQPKIKLFHKELRKLKELEHVGDVRGRGFMVGIELVKDKETKEPYPYGYKAGYRVAEKLLEKGIYMRPIGNVIILVPPLSITEKEIIYLCDALYEAIKEADL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58701</accession>
    <name>Y1305_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ1305</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ1305</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1305</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Putative antitoxin component of a putative type VII toxin-antitoxin (TA) system. Its cognate toxin might be MJ1304, which it might AMPylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64462">
        <property type="entry name" value="H64462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870822.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58701"/>
    <dbReference type="SMR" id="Q58701"/>
    <dbReference type="STRING" id="243232.MJ_1305"/>
    <dbReference type="EnsemblBacteria" id="AAB99327">
        <property type="protein sequence ID" value="AAB99327"/>
        <property type="gene ID" value="MJ_1305"/>
    </dbReference>
    <dbReference type="GeneID" id="1452207"/>
    <dbReference type="KEGG" id="mja:MJ_1305"/>
    <dbReference type="eggNOG" id="arCOG01202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131320_1_0_2"/>
    <dbReference type="InParanoid" id="Q58701"/>
    <dbReference type="OMA" id="AWYWVLK"/>
    <dbReference type="OrthoDB" id="85273at2157"/>
    <dbReference type="PhylomeDB" id="Q58701"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107263" description="Putative protein adenylyltransferase MJ1305">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="32"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="147" mass="17388" checksum="7E19C52F45D4065D" modified="1997-11-01" version="1">MDEKMLNNILDEFLQKCKQKFGDDLISIILFGSYARGTAVEYSDVDLLVIAKNLPKRRIDRHKVLRDIVLEFIYRYGINISPILVEPRDLSLKSINPLICGILTGYKIIYDRDNFWKNYLERIKPIIKRIKPIFIDEEKEWKIADLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58703</accession>
    <name>Y1307_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1307</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1307</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64463">
        <property type="entry name" value="B64463"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870824.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58703"/>
    <dbReference type="SMR" id="Q58703"/>
    <dbReference type="STRING" id="243232.MJ_1307"/>
    <dbReference type="EnsemblBacteria" id="AAB99315">
        <property type="protein sequence ID" value="AAB99315"/>
        <property type="gene ID" value="MJ_1307"/>
    </dbReference>
    <dbReference type="GeneID" id="1452209"/>
    <dbReference type="KEGG" id="mja:MJ_1307"/>
    <dbReference type="eggNOG" id="arCOG03079">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069132_3_0_2"/>
    <dbReference type="InParanoid" id="Q58703"/>
    <dbReference type="OMA" id="TAVLFDW"/>
    <dbReference type="OrthoDB" id="97304at2157"/>
    <dbReference type="PhylomeDB" id="Q58703"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107265" description="Uncharacterized protein MJ1307">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="262" mass="28591" checksum="EBA52610F60F90AB" modified="1996-11-01" version="1">MNSKRDLAVAISFFVFGASVLYSLAHMQISPGVNEVYLTHYIIPNYVCAVIFDWRAYDTLGECLVLVVAVMVSWIVFGKSLYDNTYLKELFHAPESDDYITLQGWGEYTPIIKFLAFPMSVLMVALGIITVLGGHITPGGGFQGGALIAAAFILSVIAFGSNSPLWFDHKFLEKLEALGALGYLLLGVAGMFIGGYYLFNFTEINGFTIFPAPKEIITAGIIPYLNIAVGLKVLAGLSTAAFLLSCEKVIIEKISKSEEKLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58705</accession>
    <name>Y1309_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1309</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1309</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64463">
        <property type="entry name" value="D64463"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870826.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58705"/>
    <dbReference type="SMR" id="Q58705"/>
    <dbReference type="STRING" id="243232.MJ_1309"/>
    <dbReference type="DNASU" id="1452211"/>
    <dbReference type="EnsemblBacteria" id="AAB99316">
        <property type="protein sequence ID" value="AAB99316"/>
        <property type="gene ID" value="MJ_1309"/>
    </dbReference>
    <dbReference type="GeneID" id="1452211"/>
    <dbReference type="KEGG" id="mja:MJ_1309"/>
    <dbReference type="eggNOG" id="arCOG01537">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007100_9_5_2"/>
    <dbReference type="InParanoid" id="Q58705"/>
    <dbReference type="OMA" id="YVAHHIT"/>
    <dbReference type="OrthoDB" id="28394at2157"/>
    <dbReference type="PhylomeDB" id="Q58705"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_mem"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107267" description="Uncharacterized protein MJ1309">
        <location>
            <begin position="1"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="303"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="481" mass="52543" checksum="1D04E5902DB25AE9" modified="1996-11-01" version="1">MNYLPMMIVFPLIMAIIMNLLHGKEKAVKYITFITAAILIILPFISQYGYYYFGGHGVVNGWVSGIAYLYNPAKQAIIVTLSLIASLVLITGMGEKLKNNMFVTLSLMGFASIAAIVLADDIFNLYVFFEIVSIVQAGLVFLSGTEEAYKAGLRYMIMGNVAAALMLLGIAFLLASTGTLNITDMKHYLLVDNPMIYGGLLLLIVGLAYGAGLPPFHNVKADLYARSKGFISAMLQTYSKFVLVGLMIIILKLFNGLDYFASAHAVLIALGVLAMVFGVVMALLQSDYKKLLAYHAISQGGYVATGLALGTPLGIVAGIFHAINHVIYKSALFLGAYIVSCKRGSNLHKLGGLLPLMPSVAFMVLCAKLAISGIPPFNGFQSKWMLAQAAMQVNMPEIAIIMIIVSIGTFVSMMKAFYLIYLKPVDEETLKEYQNKEVPKLAVFSLFVLTALCIIIGLYPDIVTNYLWDYAKELGVNYYLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58732</accession>
    <name>Y1336_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1336</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1336</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64466">
        <property type="entry name" value="G64466"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58732"/>
    <dbReference type="SMR" id="Q58732"/>
    <dbReference type="STRING" id="243232.MJ_1336"/>
    <dbReference type="EnsemblBacteria" id="AAB99346">
        <property type="protein sequence ID" value="AAB99346"/>
        <property type="gene ID" value="MJ_1336"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1336"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1754709_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58732"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107280" description="Uncharacterized protein MJ1336">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <sequence length="148" mass="17013" checksum="5A036EDED081B76B" modified="1996-11-01" version="1">MGYNQQLLRVDYEKIYPINDELSSKILGVIKNLNGKSDILVISDYAKGLITKELMDDIKKEFKGKILIDPKPKNDFYKDVYLIKPNLKEASQILGREIENKDDELEKSGLESVDKYNSNFVITRGEKGATLITVDEIFTMFQQKSKRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58804</accession>
    <name>Y1409_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1409</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1409</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64475">
        <property type="entry name" value="H64475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58804"/>
    <dbReference type="STRING" id="243232.MJ_1409"/>
    <dbReference type="PRIDE" id="Q58804"/>
    <dbReference type="EnsemblBacteria" id="AAB99423">
        <property type="protein sequence ID" value="AAB99423"/>
        <property type="gene ID" value="MJ_1409"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1409"/>
    <dbReference type="eggNOG" id="arCOG05083">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1048128_0_0_2"/>
    <dbReference type="OMA" id="VYIDVFM"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107314" description="Uncharacterized protein MJ1409">
        <location>
            <begin position="1"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="268" mass="31970" checksum="0FB410E4D603ECDC" modified="1996-11-01" version="1">METMKAKELAQKILLDIYRNLDEFSKDIIRGDLADIEFKGFYLKGKNGEKAYIRNLDDFENLKDFDVEMRKYKLKSINLKNLDEGLMIINLSSRVSKEYKFEANEYSIIYPSNNTTIEFKERVLKWMELEDDELDEKIIEFDTKMNEILEELLEDVEVEEEISVYIDVFMDVNKIENFVEKDDERIIIWIHPVFLFSNDDVLRGLLAYELSRFKSRFLEVGYKDIIKYCRELKKLTNKKPKVLEKIKDIANKYGDIDSLNLINEIENE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-02-23" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58807</accession>
    <name>Y1412_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0288 protein MJ1412</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1412</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0288 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64476">
        <property type="entry name" value="C64476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870929.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1412"/>
    <dbReference type="EnsemblBacteria" id="AAB99419">
        <property type="protein sequence ID" value="AAB99419"/>
        <property type="gene ID" value="MJ_1412"/>
    </dbReference>
    <dbReference type="GeneID" id="1452315"/>
    <dbReference type="KEGG" id="mja:MJ_1412"/>
    <dbReference type="eggNOG" id="arCOG04900">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_533841_0_0_2"/>
    <dbReference type="InParanoid" id="Q58807"/>
    <dbReference type="OMA" id="FKKWDVV"/>
    <dbReference type="OrthoDB" id="20383at2157"/>
    <dbReference type="PhylomeDB" id="Q58807"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01089">
        <property type="entry name" value="UPF0288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016466">
        <property type="entry name" value="UPF0288_methan"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005852">
        <property type="entry name" value="UCP005852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03268">
        <property type="entry name" value="methan_mark_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156051" description="UPF0288 protein MJ1412">
        <location>
            <begin position="1"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01089"/>
        </source>
    </evidence>
    <sequence length="498" mass="56317" checksum="D8D1DA4D2B86B166" modified="1998-06-01" version="2">MAKVIVNGKEKVGETLREVIKDEYYNEGANIVIIKGVKREAEKIPKKFLIKTTKGNITIAITENNETAKFFINNYKDFVKKLRWVSGMDVAFGSTTIDLEISTEPKEFKKWDVVLSISGLDKDEGHIVFIKKKTEGVYGLKDPKIGIVVGGKWVIDRLEVGDKIIDIEPIREEKEAVDYLVTTDLDLKLEDGWRIFTYFTAEFDGTPSAVEHCLALMEDGIFEITENTNTYVADCRLQTLKIEEGNLIDRERGFITVRNYGVGEGKVYIYRESRSSSLSHTVVGRVKEGIELIDFSDSGVLSVKXVPERLCAIGLTIEEAEEMFKKYNIEVEKEGDLENAIVVEQEPEYTLDVLKEKKVKIRGLDKNKIIVIELFEDKAPITAWYFRKTTGLTTKRVGKLHVYFKHKDIVMFKGNPEYAKGLLPENIPTDKVEPCSIGVTNMVSRYKGMIGVRLGESDKFGPTGESFEKTNIVGGIVENAEYLKNVKTGDDVYVLLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58822</accession>
    <name>RFHPS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-ribofuranosylphenol 5'-phosphate synthase</fullName>
            <ecNumber evidence="2">2.4.2.54</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="3">Beta-RFA-P synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Beta-ribofuranosylhydroxybenzene 5'-phosphate synthase</fullName>
            <shortName evidence="4">Beta-RFH-P synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1427</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="39389" last="39395">
            <title>Mechanism of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase, a key enzyme in the methanopterin biosynthetic pathway.</title>
            <authorList>
                <person name="Dumitru R.V."/>
                <person name="Ragsdale S.W."/>
            </authorList>
            <dbReference type="PubMed" id="15262968"/>
            <dbReference type="DOI" id="10.1074/jbc.M406442200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ENZYME MECHANISM</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="Biochemistry" volume="50" first="6041" last="6052">
            <title>The conversion of a phenol to an aniline occurs in the biochemical formation of the 1-(4-aminophenyl)-1-deoxy-D-ribitol moiety in methanopterin.</title>
            <authorList>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="21634403"/>
            <dbReference type="DOI" id="10.1021/bi200362w"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the condensation of 4-hydroxybenzoate (HB) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylphenol 5'-phosphate (beta-RFH-P) (PubMed:21634403). Also catalyzes the condensation of 4-aminobenzoate (pABA) with PRPP to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P) (PubMed:15262968, PubMed:21634403). Only 4-hydroxybenzoate is known to be biosynthesized by methanogenic archaea, but 4-aminobenzoate can be used as substrate by growing methanogens when it is present in the growth medium (PubMed:21634403).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-hydroxybenzoate + 5-phospho-alpha-D-ribose 1-diphosphate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:48557"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate + H(+) = 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35815"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17836"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:72778"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1 2">
            <dbReference type="Rhea" id="RHEA:35816"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">1.5 mM for PRPP</KM>
            <KM evidence="1">0.15 mM for pABA</KM>
            <Vmax evidence="1">180.0 nmol/min/mg enzyme with pABA as substrate</Vmax>
            <text evidence="1">kcat is 0.23 sec(-1) with pABA as substrate.</text>
        </kinetics>
        <phDependence>
            <text evidence="1">Optimum pH is 4.9.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Lacks any chromogenic cofactor, and the presence of pyridoxal phosphate and the mechanistically related pyruvoyl cofactors has been strictly excluded.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64478">
        <property type="entry name" value="B64478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870945.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58822"/>
    <dbReference type="SMR" id="Q58822"/>
    <dbReference type="STRING" id="243232.MJ_1427"/>
    <dbReference type="EnsemblBacteria" id="AAB99449">
        <property type="protein sequence ID" value="AAB99449"/>
        <property type="gene ID" value="MJ_1427"/>
    </dbReference>
    <dbReference type="GeneID" id="1452331"/>
    <dbReference type="KEGG" id="mja:MJ_1427"/>
    <dbReference type="eggNOG" id="arCOG01026">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061764_0_0_2"/>
    <dbReference type="InParanoid" id="Q58822"/>
    <dbReference type="OMA" id="RLNGAWY"/>
    <dbReference type="OrthoDB" id="47243at2157"/>
    <dbReference type="PhylomeDB" id="Q58822"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14546"/>
    <dbReference type="BRENDA" id="2.4.2.54">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="PTHR20861:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107320" description="Beta-ribofuranosylphenol 5'-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="15262968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21634403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="15262968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="21634403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="15262968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="21634403"/>
        </source>
    </evidence>
    <sequence length="328" mass="36198" checksum="67F166A355C41FA1" modified="1996-11-01" version="1">MIIQTPSRIHMGLIDLNGSIGRVDGGIGLALEEPNIKIEGKESDDISIEFDKKLIEKYGEDYIKSVRDRVYNTAIKVLDVIGGEGVDLKILSLFPAHSGLGSGTQLSLAVGKLISKIYNKEMNAYNIAKITGRGGTSGIGIGAFEYGGFLIDGGHSFGKGKDKEDFRPSSASKGVKPAPIIFRHDFDWETILIIPKGEHVYGKKEVDIFKKYCPVPLNEVEKICHLVLMKMMPAVVEKNLDDFGEVINKLQYLGFKKVELSLQSDIVKDLINELHKDVYAGLSSFGPTIYAFGDKKLIVEKANDIFDKYGVYGEIIITKANNVGHKIW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q58835</accession>
    <name>AROK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Shikimate kinase</fullName>
            <shortName>SK</shortName>
            <ecNumber>2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">aroK</name>
        <name type="ordered locus">MJ1440</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="J. Bacteriol." volume="183" first="292" last="300">
            <title>Archaeal shikimate kinase, a new member of the GHMP-kinase family.</title>
            <authorList>
                <person name="Daugherty M."/>
                <person name="Vonstein V."/>
                <person name="Overbeek R."/>
                <person name="Osterman A."/>
            </authorList>
            <dbReference type="PubMed" id="11114929"/>
            <dbReference type="DOI" id="10.1128/JB.183.1.292-300.2001"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + shikimate = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64479">
        <property type="entry name" value="G64479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870958.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58835"/>
    <dbReference type="SMR" id="Q58835"/>
    <dbReference type="STRING" id="243232.MJ_1440"/>
    <dbReference type="EnsemblBacteria" id="AAB99451">
        <property type="protein sequence ID" value="AAB99451"/>
        <property type="gene ID" value="MJ_1440"/>
    </dbReference>
    <dbReference type="GeneID" id="1452344"/>
    <dbReference type="KEGG" id="mja:MJ_1440"/>
    <dbReference type="eggNOG" id="arCOG01025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073768_0_0_2"/>
    <dbReference type="InParanoid" id="Q58835"/>
    <dbReference type="OMA" id="WDVLVWT"/>
    <dbReference type="OrthoDB" id="98200at2157"/>
    <dbReference type="PhylomeDB" id="Q58835"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-18801"/>
    <dbReference type="SABIO-RK" id="Q58835"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="PTHR20861:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="SSF55060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141574" description="Shikimate kinase">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11114929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="11114929"/>
        </source>
    </evidence>
    <sequence length="282" mass="30598" checksum="20EDBD4B736AE011" modified="1996-11-01" version="1">MEGKAYALASGTIINAIATGKGSAFGLDLKVYAKVKLIDDGKNKIEGKVLDNPNIKPNLIVRCVKNTLDYFGLNYSAYVETKTEIPIKSGLSSSSATSNAVVLATFDALGEKIDDELILNLGIKSSFDEKLTVTGAYDDATASYYGGITITDNIERKILKRDKMRDDLNVLILIPNLEKNVDVNRMKLIKDYVEIAFNEAINGNYFKALFLNGILYASALNFPTNIAIDALDAGAITAGLSGTGPSYIAMVEDENVEKVKEKLNRYGKVILTKPNNDGASIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58840</accession>
    <name>Y1445_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1445</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1445</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64480">
        <property type="entry name" value="D64480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870965.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58840"/>
    <dbReference type="SMR" id="Q58840"/>
    <dbReference type="STRING" id="243232.MJ_1445"/>
    <dbReference type="EnsemblBacteria" id="AAB99460">
        <property type="protein sequence ID" value="AAB99460"/>
        <property type="gene ID" value="MJ_1445"/>
    </dbReference>
    <dbReference type="GeneID" id="1452349"/>
    <dbReference type="KEGG" id="mja:MJ_1445"/>
    <dbReference type="eggNOG" id="arCOG01154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092313_3_1_2"/>
    <dbReference type="InParanoid" id="Q58840"/>
    <dbReference type="OMA" id="WIIHGHH"/>
    <dbReference type="OrthoDB" id="95552at2157"/>
    <dbReference type="PhylomeDB" id="Q58840"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107336" description="Uncharacterized protein MJ1445">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <sequence length="176" mass="20713" checksum="B5D5F69B2896A38B" modified="1996-11-01" version="1">MSMRKIYLISDTHFNHANIIKYCNRPFSNVEEMNKTLIKNWNNVVRDKDIVYFLGDLILSKNKAKKARELLELLNGEIVFIRGNHDKFGEKFRVIEYNGYKFMLIHNPDSSYTLNFDGWIIHGHHHANHLDEYPFINPKRKSINVSVEVINYKPVSLDLIVKLIEKGKVVRTINDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58849</accession>
    <name>AROD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-dehydroquinate dehydratase</fullName>
            <shortName evidence="1">3-dehydroquinase</shortName>
            <ecNumber evidence="1">4.2.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type I DHQase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Type I dehydroquinase</fullName>
            <shortName evidence="1">DHQ1</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroD</name>
        <name type="ordered locus">MJ1454</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the third step of the chorismate pathway, which leads to the biosynthesis of aromatic amino acids. Catalyzes the cis-dehydration of 3-dehydroquinate (DHQ) and introduces the first double bond of the aromatic ring to yield 3-dehydroshikimate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.10" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64481">
        <property type="entry name" value="E64481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870974.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58849"/>
    <dbReference type="SMR" id="Q58849"/>
    <dbReference type="STRING" id="243232.MJ_1454"/>
    <dbReference type="EnsemblBacteria" id="AAB99464">
        <property type="protein sequence ID" value="AAB99464"/>
        <property type="gene ID" value="MJ_1454"/>
    </dbReference>
    <dbReference type="GeneID" id="1452358"/>
    <dbReference type="KEGG" id="mja:MJ_1454"/>
    <dbReference type="eggNOG" id="arCOG02097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064444_2_1_2"/>
    <dbReference type="InParanoid" id="Q58849"/>
    <dbReference type="OMA" id="ATMAMGE"/>
    <dbReference type="OrthoDB" id="75928at2157"/>
    <dbReference type="PhylomeDB" id="Q58849"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00086"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046279">
        <property type="term" value="P:3,4-dihydroxybenzoate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00214">
        <property type="entry name" value="AroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018508">
        <property type="entry name" value="3-dehydroquinate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01028">
        <property type="entry name" value="DEHYDROQUINASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000138829" description="3-dehydroquinate dehydratase">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>3-dehydroquinate</name>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00214"/>
        </source>
    </evidence>
    <sequence length="220" mass="25045" checksum="D607C5D02FE39363" modified="1996-11-01" version="1">MICLPVVEDSVEKAIKTAEKYLEIADIVEFRIDMLKEVSEEDIEKFAKYPCIITVRADWEGGYWKGNNEERLNLIKKAIECNAKFVDIELREEKNKELVKFRDEIGSKTKIIISYHDFEKTPSKEKLVEIVEKALSIGDIAKFATMANSKEDVLNILEVINKYPGKIIGIGMGEKGKLTRILGVYFGSILTFASYKGKSSAPGQVDIDTLKEIWRLMDLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58858</accession>
    <name>Y1463_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0128 protein MJ1463</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1463</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0128 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64482">
        <property type="entry name" value="F64482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870983.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58858"/>
    <dbReference type="STRING" id="243232.MJ_1463"/>
    <dbReference type="EnsemblBacteria" id="AAB99477">
        <property type="protein sequence ID" value="AAB99477"/>
        <property type="gene ID" value="MJ_1463"/>
    </dbReference>
    <dbReference type="GeneID" id="1452367"/>
    <dbReference type="KEGG" id="mja:MJ_1463"/>
    <dbReference type="eggNOG" id="arCOG05084">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1243079_0_0_2"/>
    <dbReference type="InParanoid" id="Q58858"/>
    <dbReference type="OMA" id="FGRFVFQ"/>
    <dbReference type="OrthoDB" id="58756at2157"/>
    <dbReference type="PhylomeDB" id="Q58858"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00264">
        <property type="entry name" value="UPF0128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005266">
        <property type="entry name" value="UPF0128"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03673">
        <property type="entry name" value="UPF0128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016179">
        <property type="entry name" value="UCP016179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00703">
        <property type="entry name" value="TIGR00703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000185225" description="UPF0128 protein MJ1463">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="233" mass="26609" checksum="DDB46A3B1D893999" modified="1996-11-01" version="1">MVVDAKEVEMINTLVFETLGNPEKEREFKLKSLKRWGFDLIFGKVDGKETYFTVELDERKAGDKFSKDGKEYEVIEVLQELPKNTELYAHIEMEMGKAYIVCQLRDEDGKNTEVLRVPAATLLLAFLKKNKLANIIKAIKNVGISLELSMQNGVGGKPLSYEELPNVARRFIRSARKVEKETGFGRLSFAYYGETKDGEPRYRFSWLLPTIALFDLDIAKKVEQTLGILKVSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q58938</accession>
    <name>ILVC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="1">KARI</shortName>
            <ecNumber evidence="1">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="1">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ketol-acid reductoisomerase type I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvC</name>
        <name type="ordered locus">MJ1543</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99561" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99561.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64492">
        <property type="entry name" value="F64492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496863.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58938"/>
    <dbReference type="SMR" id="Q58938"/>
    <dbReference type="STRING" id="243232.MJ_1543"/>
    <dbReference type="EnsemblBacteria" id="AAB99561">
        <property type="protein sequence ID" value="AAB99561"/>
        <property type="gene ID" value="MJ_1543"/>
    </dbReference>
    <dbReference type="GeneID" id="1452451"/>
    <dbReference type="KEGG" id="mja:MJ_1543"/>
    <dbReference type="eggNOG" id="arCOG04465">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033821_0_1_2"/>
    <dbReference type="InParanoid" id="Q58938"/>
    <dbReference type="OMA" id="RAMFSWL"/>
    <dbReference type="OrthoDB" id="25435at2157"/>
    <dbReference type="PhylomeDB" id="Q58938"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="PTHR21371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="IlvN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151391" description="Ketol-acid reductoisomerase (NADP(+))">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="2">
        <location>
            <begin position="2"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="3">
        <location>
            <begin position="183"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
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            <name>NADP(+)</name>
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            <position position="48"/>
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            <name>NADP(+)</name>
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            <position position="53"/>
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            <name>NADP(+)</name>
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            <name>NADP(+)</name>
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            <position position="191"/>
        </location>
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            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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        </ligand>
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            <position position="191"/>
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        <location>
            <position position="227"/>
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            <position position="231"/>
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        </ligand>
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            <position position="252"/>
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            <name>substrate</name>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01197"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="330" mass="36827" checksum="B3B3B0A3BC0E027B" modified="2006-10-17" version="3">MVKIFYDKDVTFDAVKDKTIAVIGYGSQGRAQALNMKDSGLNVIVGLRPNGASWNKAIKDGHKVMTIEEAAEKADIIHILIPDEVQPAVYKKQIEPYLTEGKTISFSHGYNIHYGFIRPPENVNITMVAPKSPGAMVRKTYEEGFGVPGLVAVERDYTGDALQIALGMAKGIGLTKVGVIQTTFREETETDLFGEQVVLCGGVTELIKAAFETLVEAGYAPEMAYFETCHELKLIVDLIYQKGLQGMWENVSNTAEYGGLTRRARVINEESRKAMKEILKEIQDGRFAKEWSLEREAGFPHLNALRRLEKEHLIEKVGKELRKMCGLEKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58949</accession>
    <name>Y1554_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1554</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1554</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64494">
        <property type="entry name" value="A64494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871078.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58949"/>
    <dbReference type="STRING" id="243232.MJ_1554"/>
    <dbReference type="EnsemblBacteria" id="AAB99574">
        <property type="protein sequence ID" value="AAB99574"/>
        <property type="gene ID" value="MJ_1554"/>
    </dbReference>
    <dbReference type="GeneID" id="1452462"/>
    <dbReference type="KEGG" id="mja:MJ_1554"/>
    <dbReference type="eggNOG" id="arCOG02284">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015706_1_0_2"/>
    <dbReference type="InParanoid" id="Q58949"/>
    <dbReference type="OMA" id="TYLHPIG"/>
    <dbReference type="OrthoDB" id="4397at2157"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019198">
        <property type="entry name" value="Beta_propeller_containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011044">
        <property type="entry name" value="Quino_amine_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014441">
        <property type="entry name" value="UCP006425_b-propeller"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09826">
        <property type="entry name" value="Beta_propel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006425">
        <property type="entry name" value="UCP006425_WD40"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50969">
        <property type="entry name" value="SSF50969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014017" description="Uncharacterized protein MJ1554">
        <location>
            <begin position="21"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00303"/>
        </source>
    </evidence>
    <sequence length="622" mass="72283" checksum="86CAA62F3854D73F" modified="1996-11-01" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58960</accession>
    <name>Y1565_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable helicase MJ1565</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MJ1565</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="D64495"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871089.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58960"/>
    <dbReference type="SMR" id="Q58960"/>
    <dbReference type="STRING" id="243232.MJ_1565"/>
    <dbReference type="DNASU" id="1452473"/>
    <dbReference type="EnsemblBacteria" id="AAB99585">
        <property type="protein sequence ID" value="AAB99585"/>
        <property type="gene ID" value="MJ_1565"/>
    </dbReference>
    <dbReference type="GeneID" id="1452473"/>
    <dbReference type="KEGG" id="mja:MJ_1565"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="InParanoid" id="Q58960"/>
    <dbReference type="OMA" id="CLVSQRP"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="PhylomeDB" id="Q58960"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033186">
        <property type="entry name" value="HerA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="PTHR42957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05872">
        <property type="entry name" value="DUF853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107413" description="Probable helicase MJ1565">
        <location>
            <begin position="1"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="160"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="467"/>
            <end position="468"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="F2Z5Z6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q97WG8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="AAB99585.1"/>
        </source>
    </evidence>
    <sequence length="513" mass="58672" checksum="A45C1EAD7D932526" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58983</accession>
    <name>Y1588_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1588</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1588</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64498">
        <property type="entry name" value="C64498"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58983"/>
    <dbReference type="STRING" id="243232.MJ_1588"/>
    <dbReference type="EnsemblBacteria" id="AAB99609">
        <property type="protein sequence ID" value="AAB99609"/>
        <property type="gene ID" value="MJ_1588"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1588"/>
    <dbReference type="eggNOG" id="arCOG00306">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2140208_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107429" description="Uncharacterized protein MJ1588">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="112" mass="12800" checksum="0CEC073B0907E14E" modified="1996-11-01" version="1">MVGNAYTLFEGNSADDLYKAIVKKRTTYEGKPTPLYQAILWSYKVVYTSEKKLIKSLIFRIGDNTIDSIKLYKKILGVFGGFIYILTPLPIVSGFLGNYYLKKKAKEKMKEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58994</accession>
    <name>Y1599_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1599</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1599</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64499">
        <property type="entry name" value="F64499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871124.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58994"/>
    <dbReference type="SMR" id="Q58994"/>
    <dbReference type="STRING" id="243232.MJ_1599"/>
    <dbReference type="PRIDE" id="Q58994"/>
    <dbReference type="DNASU" id="1452508"/>
    <dbReference type="EnsemblBacteria" id="AAB99626">
        <property type="protein sequence ID" value="AAB99626"/>
        <property type="gene ID" value="MJ_1599"/>
    </dbReference>
    <dbReference type="GeneID" id="1452508"/>
    <dbReference type="KEGG" id="mja:MJ_1599"/>
    <dbReference type="eggNOG" id="arCOG00046">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1014190_0_0_2"/>
    <dbReference type="InParanoid" id="Q58994"/>
    <dbReference type="OMA" id="GYTTNCL"/>
    <dbReference type="OrthoDB" id="25986at2157"/>
    <dbReference type="PhylomeDB" id="Q58994"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012121">
        <property type="entry name" value="ATPase_PP-loop_MJ1599"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036668">
        <property type="entry name" value="ATPase_UCP036668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107433" description="Uncharacterized protein MJ1599">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <sequence length="277" mass="32340" checksum="F59EF4A2485D3BAE" modified="1996-11-01" version="1">MKCSICVHTSKTKKIINYEGKPICVDCLTMLKYPPNFEKMKKEVEEILYNLKKEGGKYHCILAFSGGKDSVLALKLLKEKFKLNPLCVMVDNKYMAKEAIENALNVTKHYQVDLMILNRDYTDLFEDAIKRGESPCRRCSRLILREVWRVTKLLGLKYIITGHELPFGHSAIREMKEGIKMIRLLAPYKFKEEEKYKMLEDLPWKKPDLGGYTTNCLVLGVALERFYDKYGFSFEIDRIATLVRLGLLSKEKAKKELEKPKVPKEIYEELRRRGLKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q58998</accession>
    <name>RPIA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="1">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphoriboisomerase A</fullName>
            <shortName evidence="1">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpiA</name>
        <name type="ordered locus">MJ1603</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Bacteriol." volume="187" first="7382" last="7389">
            <title>Ribose-5-phosphate biosynthesis in Methanocaldococcus jannaschii occurs in the absence of a pentose-phosphate pathway.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="16237021"/>
            <dbReference type="DOI" id="10.1128/JB.187.21.7382-7389.2005"/>
        </citation>
        <scope>PATHWAY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Acta Crystallogr. F Struct. Biol. Commun." volume="65" first="1214" last="1217">
            <title>The structure of an archaeal ribose-5-phosphate isomerase from Methanocaldococcus jannaschii (MJ1603).</title>
            <authorList>
                <person name="Strange R.W."/>
                <person name="Antonyuk S.V."/>
                <person name="Ellis M.J."/>
                <person name="Bessho Y."/>
                <person name="Kuramitsu S."/>
                <person name="Yokoyama S."/>
                <person name="Hasnain S.S."/>
            </authorList>
            <dbReference type="PubMed" id="20054114"/>
            <dbReference type="DOI" id="10.1107/S1744309109044923"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.78 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOGS</scope>
        <scope>ACTIVE SITE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate biosynthesis; D-ribose 5-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64500">
        <property type="entry name" value="B64500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871128.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3IXQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.78 A"/>
        <property type="chains" value="A/B/C/D=1-226"/>
    </dbReference>
    <dbReference type="PDBsum" id="3IXQ"/>
    <dbReference type="AlphaFoldDB" id="Q58998"/>
    <dbReference type="SMR" id="Q58998"/>
    <dbReference type="STRING" id="243232.MJ_1603"/>
    <dbReference type="EnsemblBacteria" id="AAB99623">
        <property type="protein sequence ID" value="AAB99623"/>
        <property type="gene ID" value="MJ_1603"/>
    </dbReference>
    <dbReference type="GeneID" id="1452512"/>
    <dbReference type="KEGG" id="mja:MJ_1603"/>
    <dbReference type="eggNOG" id="arCOG01122">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_1_1_2"/>
    <dbReference type="InParanoid" id="Q58998"/>
    <dbReference type="OMA" id="YDWDEVN"/>
    <dbReference type="OrthoDB" id="98657at2157"/>
    <dbReference type="PhylomeDB" id="Q58998"/>
    <dbReference type="BRENDA" id="5.3.1.6">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00293"/>
    <dbReference type="EvolutionaryTrace" id="Q58998"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="PTHR11934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="SSF100950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158510" description="Ribose-5-phosphate isomerase A">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="97"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="4"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="23"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="51"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="57"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="73"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="79"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="86"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="113"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="135"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="141"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="164"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="177"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="188"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="201"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="211"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="220"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16237021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="20054114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="20054114"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3IXQ"/>
        </source>
    </evidence>
    <sequence length="226" mass="24830" checksum="C0F1C2C1A5499123" modified="1996-11-01" version="1">MSNEDLKLKVAKEAVKLVKDGMVIGLGTGSTAALFIRELGNRIREEELTVFGIPTSFEAKMLAMQYEIPLVTLDEYDVDIAFDGADEVEETTLFLIKGGGGCHTQEKIVDYNANEFVVLVDESKLVKKLGEKFPIPVEVIPSAYRVVIRALSEMGGEAVIRLGDRKRGPVITDNGNMIIDVFMNIDDAIELEKEINNIPGVVENGIFTKVDKVLVGTKKGVKTLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q59005</accession>
    <name>Y1610_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized glycosyl hydrolase MJ1610</fullName>
            <ecNumber>3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1610</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 15 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64501">
        <property type="entry name" value="A64501"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59005"/>
    <dbReference type="SMR" id="Q59005"/>
    <dbReference type="STRING" id="243232.MJ_1610"/>
    <dbReference type="CAZy" id="GH15">
        <property type="family name" value="Glycoside Hydrolase Family 15"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99630">
        <property type="protein sequence ID" value="AAB99630"/>
        <property type="gene ID" value="MJ_1610"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1610"/>
    <dbReference type="eggNOG" id="arCOG03285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028187_0_0_2"/>
    <dbReference type="InParanoid" id="Q59005"/>
    <dbReference type="OMA" id="GSWFQRY"/>
    <dbReference type="PhylomeDB" id="Q59005"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004339">
        <property type="term" value="F:glucan 1,4-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005976">
        <property type="term" value="P:polysaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000165">
        <property type="entry name" value="Glucoamylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006465">
        <property type="entry name" value="Oligosac_amylase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="SSF48208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01577">
        <property type="entry name" value="oligosac_amyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00820">
        <property type="entry name" value="GLUCOAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000186121" description="Uncharacterized glycosyl hydrolase MJ1610">
        <location>
            <begin position="1"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="403"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="615" mass="72008" checksum="2B37EB89F0357BE5" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q59030</accession>
    <name>MCH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methenyltetrahydromethanopterin cyclohydrolase</fullName>
            <ecNumber>3.5.4.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Methenyl-H4MPT cyclohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mch</name>
        <name type="ordered locus">MJ1636</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible interconversion of 5-formyl-H(4)MPT to methenyl-H(4)MPT(+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = H(+) + N(5)-formyl-5,6,7,8-tetrahydromethanopterin</text>
            <dbReference type="Rhea" id="RHEA:19053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="3.5.4.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.27"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64504">
        <property type="entry name" value="B64504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871160.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59030"/>
    <dbReference type="SMR" id="Q59030"/>
    <dbReference type="STRING" id="243232.MJ_1636"/>
    <dbReference type="EnsemblBacteria" id="AAB99657">
        <property type="protein sequence ID" value="AAB99657"/>
        <property type="gene ID" value="MJ_1636"/>
    </dbReference>
    <dbReference type="GeneID" id="1452545"/>
    <dbReference type="KEGG" id="mja:MJ_1636"/>
    <dbReference type="eggNOG" id="arCOG02675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_876031_0_0_2"/>
    <dbReference type="InParanoid" id="Q59030"/>
    <dbReference type="OMA" id="DKGMFAP"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="PhylomeDB" id="Q59030"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00694"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018759">
        <property type="term" value="F:methenyltetrahydromethanopterin cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00545">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00486">
        <property type="entry name" value="McH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003209">
        <property type="entry name" value="METHMP_CycHdrlase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02289">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03120">
        <property type="entry name" value="one_C_mch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140881" description="Methenyltetrahydromethanopterin cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="323" mass="34900" checksum="C793B6CF432BA31F" modified="1996-11-01" version="1">MLSVNKKALEIVNKMIENKEEINIDVIKLENGATVLDCGVNVPGSWKAGKLFTKICLGGLAHVGISLSPCECKGITLPYVKIKTSHPAIATLGAQKAGWAVKVGKYFAMGSGPARALAKKPKKTYEEIGYEDDADVAVLCLEASKLPNEEVAEYVAKECGVEVENVYLLVAPTASLVGSIQISGRVVENGTYKMLEVLEFDVNKVKYAAGLAPIAPIIGDDFAMMGATNDMVLYGGITYYYIKSDENDDIESLCKALPSCASKDYGKPFMEVFKAADYDFYKIDKGMFAPAVVVINDMTTGKVYRAGKVNAEVLKKSLGWTEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q59031</accession>
    <name>Y1637_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1637</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1637</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64504">
        <property type="entry name" value="C64504"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59031"/>
    <dbReference type="STRING" id="243232.MJ_1637"/>
    <dbReference type="DNASU" id="1452546"/>
    <dbReference type="EnsemblBacteria" id="AAB99658">
        <property type="protein sequence ID" value="AAB99658"/>
        <property type="gene ID" value="MJ_1637"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1637"/>
    <dbReference type="eggNOG" id="arCOG03167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044680_0_0_2"/>
    <dbReference type="InParanoid" id="Q59031"/>
    <dbReference type="OMA" id="KIFLHNT"/>
    <dbReference type="PhylomeDB" id="Q59031"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041682">
        <property type="entry name" value="AAA_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13173">
        <property type="entry name" value="AAA_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014019" description="Uncharacterized protein MJ1637">
        <location>
            <begin position="20"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="473" mass="55322" checksum="08E8FA079D540C42" modified="1996-11-01" version="1" precursor="true">MIRAFLVFPYLYILVQSNGYTGGIMVQLNDYILNHIAKTPLILSRYNKEKKRFDYDILKEKVDKYIENDKKELILLYGLRGLGKTTLLSQIYHYARLKIEPNRVLYFSMDELKLNDIKLMDALKAYSEIFGINLYEEKIILLLDEIQYERHWDLVLKNLYDTTNIFIIATGSSALKLRESPDLARRALHKPIYPMTFREYLYLTKNIKIESLFEEVILNNNLDNFKKVYAKVYSQILEEDVKKYLRIGSLPFALEDDELEVYNKIYTMLERIIYKDVREVKEFDMETLDKAFKLLYLLANPKGERYSYESLANTLEIAKGTLINLVDVLEKCELLFKIYPYGSMDKKVRKSQKIKFLPVPIKTALWHKMGVVIDDVCYGSLLEDVVAFYLYLFCKKKGYSLSYEPKKGGADFILISPYMEKIVIEVGLGKKSSKQVLKSMERVKASKGIIIGDELKVEGNILYIPWKGFLLLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q59048</accession>
    <name>Y1654_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1654</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1654</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64506">
        <property type="entry name" value="D64506"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59048"/>
    <dbReference type="SMR" id="Q59048"/>
    <dbReference type="STRING" id="243232.MJ_1654"/>
    <dbReference type="EnsemblBacteria" id="AAB99683">
        <property type="protein sequence ID" value="AAB99683"/>
        <property type="gene ID" value="MJ_1654"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1654"/>
    <dbReference type="eggNOG" id="arCOG02261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1335077_0_0_2"/>
    <dbReference type="OMA" id="RYVAYKI"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107460" description="Uncharacterized protein MJ1654">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="PCI">
        <location>
            <begin position="166"/>
            <end position="214"/>
        </location>
    </feature>
    <sequence length="225" mass="26418" checksum="82D66A10FA1B43D2" modified="1996-11-01" version="1">MGYPTPIKLGLSAPINVPLIYKTGEKMRYVAYKIYPEEFLNNEVVDNALIIEGRKVRRVRILGKVENINVGNIISFYVDGVNVRYFEEKPVYIEEGDIVDVIGRPRTYDGEKYIMAEIIRKRDERWIKLRDLEIKKTRKYLLERAELYEEENEEMSLEEEVYTEILNSDVIKDKILAIIENVGEITYEELAEKINIPEEDLEKYLSELKESGDIFEPRPGVYKVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q59070</accession>
    <name>Y1676_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1676</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1676</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH564.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64509">
        <property type="entry name" value="B64509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871200.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59070"/>
    <dbReference type="SMR" id="Q59070"/>
    <dbReference type="STRING" id="243232.MJ_1676"/>
    <dbReference type="EnsemblBacteria" id="AAB99698">
        <property type="protein sequence ID" value="AAB99698"/>
        <property type="gene ID" value="MJ_1676"/>
    </dbReference>
    <dbReference type="GeneID" id="1452585"/>
    <dbReference type="KEGG" id="mja:MJ_1676"/>
    <dbReference type="eggNOG" id="arCOG04839">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1149814_0_0_2"/>
    <dbReference type="OMA" id="AFDTYGG"/>
    <dbReference type="OrthoDB" id="88910at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012021">
        <property type="entry name" value="UCP005278"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005278">
        <property type="entry name" value="UCP005278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107473" description="Uncharacterized protein MJ1676">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="238" mass="26085" checksum="4A2656C01EAF76C1" modified="1996-11-01" version="1">MKKYIVSIGVDISDNDVKTNPKVNELVNKEIKKRLSKLGIKATISNITGDDIVITSFVPENLIEKNNKIIFEVLNKYAEGFDDLRGISEDKDKAGEGLSYAIAESISEYGDAIIIAFDTYGGESFVDEMALFVKEIGEKFGYDVGCSVSNEPIEIPGIGYTGAESDDPVVVITVEELDDIPKLAGLIYGGLLSFDKLYFVKNGDEVNILPPGVIYTMTAFLNGNVIDLYGGIRRKIKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q60172</accession>
    <name>HEM1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="1">GluTR</shortName>
            <ecNumber evidence="1">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hemA</name>
        <name type="ordered locus">MJ0143</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-4-amino-5-oxopentanoate + NADP(+) + tRNA(Glu) = H(+) + L-glutamyl-tRNA(Glu) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64317">
        <property type="entry name" value="H64317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869638.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60172"/>
    <dbReference type="SMR" id="Q60172"/>
    <dbReference type="STRING" id="243232.MJ_0143"/>
    <dbReference type="PRIDE" id="Q60172"/>
    <dbReference type="EnsemblBacteria" id="AAB98126">
        <property type="protein sequence ID" value="AAB98126"/>
        <property type="gene ID" value="MJ_0143"/>
    </dbReference>
    <dbReference type="GeneID" id="1450987"/>
    <dbReference type="KEGG" id="mja:MJ_0143"/>
    <dbReference type="eggNOG" id="arCOG01036">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035113_0_0_2"/>
    <dbReference type="InParanoid" id="Q60172"/>
    <dbReference type="OMA" id="CELKAME"/>
    <dbReference type="OrthoDB" id="48038at2157"/>
    <dbReference type="PhylomeDB" id="Q60172"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
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        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:protoporphyrinogen IX biosynthetic process from glutamate"/>
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        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
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        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="SSF69075"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="SSF69742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000114101" description="Glutamyl-tRNA reductase">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="39"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="91"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="165"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <sequence length="392" mass="44847" checksum="952E1CFDCDA3874C" modified="1997-11-01" version="1">MIILKADYKKYNVSELEKLRMDEEKFYETFDNAILLQTCNRVEIIFDADSLEEIKGIENIDLEKFDILFGDKAIEHLFRVACGLESMIVGEDQILGQLKNAYLKAKEKGRISKKLEKIILKAIHTGQRARVETKINEGGVSIGSAAVELAEKIFGLEGKNVLLIGAGEMANLVIKALKEKNIKAIIVANRTYEKAEKLAKELGGMAIKFDKLEEALRYADIVISATGAPHPILNKERLKNAGKTIIIDIANPRDTTDDIRELPDIFLFTIDDLRLVAEENLKKRKEEIPKVEMIICEELERLKEFLDKMRFETAIKELGQYIENVRKKEVEKAKKILKNKNKPVEEVLEDFSKALCKRIIYDIIKIFENVEDKEVFECLAKEFKKLGNKNKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q60174</accession>
    <name>ASSY_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate synthase</fullName>
            <ecNumber evidence="1">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argG</name>
        <name type="ordered locus">MJ0429</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64353">
        <property type="entry name" value="E64353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869928.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60174"/>
    <dbReference type="SMR" id="Q60174"/>
    <dbReference type="STRING" id="243232.MJ_0429"/>
    <dbReference type="EnsemblBacteria" id="AAB98414">
        <property type="protein sequence ID" value="AAB98414"/>
        <property type="gene ID" value="MJ_0429"/>
    </dbReference>
    <dbReference type="GeneID" id="1451289"/>
    <dbReference type="KEGG" id="mja:MJ_0429"/>
    <dbReference type="eggNOG" id="arCOG00112">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032784_4_2_2"/>
    <dbReference type="InParanoid" id="Q60174"/>
    <dbReference type="OMA" id="QCEVVTF"/>
    <dbReference type="OrthoDB" id="49443at2157"/>
    <dbReference type="PhylomeDB" id="Q60174"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="Argininosuccinate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="PTHR11587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="SSF69864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148676" description="Argininosuccinate synthase">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <sequence length="395" mass="44723" checksum="655D4A7AC06E7A7F" modified="1997-11-01" version="1">MERIAVLAYSGGLDTSCCLKLLEDKYGYKVVSVCVDVGQPEEEIKEVEEKAKKLGVLKHYTIDAKEEFVKDYIFRAIKANAMYEGYPLSTALARPLIAHKVVEIAEEVGAEAVAHGCTGKGNDQFRFETTIRIKAPHLKIIAPIRDLNLTRAEEIEYAKEKGIPIPTESKKYSIDENLWGRSIEGSELENPDFVPPEEIYAWTKNPVEDKEEEIVEIEFKEGVPVAINGEKLEPVELIKKANEIAGKHGVGRIDIIEDRIIGLKSRENYECPGAVLLLTAHKALEQLVLTRDELRFKEIVDSLYGELIYKGLWFDPLREDLDAFIDKTQERVTGTVKVKLFGGTARVVGRDSPYALYSKELVSFDEKEIDQKELAGMVKYHGLQAMLYEMRKKRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q60277</accession>
    <name>Y3515_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJECL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL15</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64511">
        <property type="entry name" value="G64511"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890063.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="PDB" id="3H92">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=274-365"/>
    </dbReference>
    <dbReference type="PDBsum" id="3H92"/>
    <dbReference type="AlphaFoldDB" id="Q60277"/>
    <dbReference type="SMR" id="Q60277"/>
    <dbReference type="STRING" id="243232.MJ_ECL15"/>
    <dbReference type="PRIDE" id="Q60277"/>
    <dbReference type="EnsemblBacteria" id="AAC37088">
        <property type="protein sequence ID" value="AAC37088"/>
        <property type="gene ID" value="MJ_ECL15"/>
    </dbReference>
    <dbReference type="GeneID" id="1450801"/>
    <dbReference type="KEGG" id="mja:MJ_ECL15"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q60277"/>
    <dbReference type="OMA" id="INEMRYK"/>
    <dbReference type="OrthoDB" id="44502at2157"/>
    <dbReference type="PhylomeDB" id="Q60277"/>
    <dbReference type="EvolutionaryTrace" id="Q60277"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184679" description="Uncharacterized ATP-binding protein MJECL15">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="281"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="306"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="320"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="324"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="329"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="340"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="345"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="349"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="355"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3H92"/>
        </source>
    </evidence>
    <sequence length="365" mass="43299" checksum="C3D54F7DCD1A8789" modified="1997-11-01" version="1">MKFFNREKEINEILLILEEEPNNIYFIYGPLNSGKSTLIREVITNRLDKSKYIPFFIDFRTRNILNVDNFIECLFEVDEKSKIDDFREYAKSLADLLVKGSEEISKYYLGMPIKVPKPFFDRIFSKRDKSADVYQYIEYLFAKLNEKGKKPILIFDELQMIREITLNGNRLLLWSLFQFLVALTKVQHLCHVFCLSSDSLFIEYIYGKAELKGGVDYILVDDFDKKTALKFMDFLAKQKNINLTNEDKELIYSYVGGKAKYIYDVIVKLKAVKDLKYILETKLEEERNHLEELLEKVEEDYEGINYDEVLEALKLFKDNYELPKSKIKRKIRIFLIKENILFLNPQKGTLKPQSYLVWNAIKRML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q60290</accession>
    <name>Y3533_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL33</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64513">
        <property type="entry name" value="H64513"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60290"/>
    <dbReference type="EnsemblBacteria" id="AAC37102">
        <property type="protein sequence ID" value="AAC37102"/>
        <property type="gene ID" value="MJ_ECL33"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_ECL33"/>
    <dbReference type="eggNOG" id="arCOG06586">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712940_0_0_2"/>
    <dbReference type="InParanoid" id="Q60290"/>
    <dbReference type="OMA" id="LEVTPWW"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107517" description="Uncharacterized protein MJECL33">
        <location>
            <begin position="1"/>
            <end position="408"/>
        </location>
    </feature>
    <sequence length="408" mass="48364" checksum="8E05C11F03D844A9" modified="1997-11-01" version="1">MIIMDTIFVGSAIPDITNRPQLYEWLERNMERSYKDILDDTKLEPEQNVVKTYILESNIHPKKLSRIIKTGKIKPLDEYEFYMLRIGDEGYFFIDAKNPRFWKLYTLQKSEESDKYFNKLISPIKSRLDNLWMPTGDLEKFLKKADYVRGLSTKHDETPFYLDNDVEERANKLSISSNGESVYELIKIIQSLSSDEIQEFEKLIKDFQLLNDTKIKKIFNTIKKLHEFKHLMRITKSKIKIVSEDDKDKFVLEDIYYWGKFTVKGTSIEKHNEIVDKTIENYEQKIEIIEDSLIDYTSSDWGDRIPLIYEFEKEIEDLKNFVEGLISVKEPFKIWGLAKQVEEDMYYISGVDLHNGDRFSLEVTPWWMRLYLPKGSCGNTALRLLSNIQQTYDSETILEVEKYGERIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q60317</accession>
    <name>AAT_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable aspartate aminotransferase</fullName>
            <shortName>AspAT</shortName>
            <ecNumber>2.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Transaminase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0001</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2-oxoglutarate + L-aspartate = L-glutamate + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64300">
        <property type="entry name" value="A64300"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869494.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60317"/>
    <dbReference type="SMR" id="Q60317"/>
    <dbReference type="STRING" id="243232.MJ_0001"/>
    <dbReference type="EnsemblBacteria" id="AAB97984">
        <property type="protein sequence ID" value="AAB97984"/>
        <property type="gene ID" value="MJ_0001"/>
    </dbReference>
    <dbReference type="GeneID" id="1450840"/>
    <dbReference type="KEGG" id="mja:MJ_0001"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="InParanoid" id="Q60317"/>
    <dbReference type="OMA" id="SVAMTGW"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="PhylomeDB" id="Q60317"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123858" description="Probable aspartate aminotransferase">
        <location>
            <begin position="1"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="375" mass="42396" checksum="31349B11FC9B83A9" modified="1997-11-01" version="1">MISSRCKNIKPSAIREIFNLATSDCINLGIGEPDFDTPKHIIEAAKRALDEGKTHYSPNNGIPELREEISNKLKDDYNLDVDKDNIIVTCGASEALMLSIMTLIDRGDEVLIPNPSFVSYFSLTEFAEGKIKNIDLDENFNIDLEKVKESITKKTKLIIFNSPSNPTGKVYDKETIKGLAEIAEDYNLIIVSDEVYDKIIYDKKHYSPMQFTDRCILINGFSKTYAMTGWRIGYLAVSDELNKELDLINNMIKIHQYSFACATTFAQYGALAALRGSQKCVEDMVREFKMRRDLIYNGLKDIFKVNKPDGAFYIFPDVSEYGDGVEVAKKLIENKVLCVPGVAFGENGANYIRFSYATKYEDIEKALGIIKEIFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q60336</accession>
    <name>ARTE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable archaeosortase E</fullName>
            <ecNumber evidence="1">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">artE</name>
        <name type="ordered locus">MJ0027</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="J. Bacteriol." volume="194" first="36" last="48">
            <title>Archaeosortases and exosortases are widely distributed systems linking membrane transit with posttranslational modification.</title>
            <authorList>
                <person name="Haft D.H."/>
                <person name="Payne S.H."/>
                <person name="Selengut J.D."/>
            </authorList>
            <dbReference type="PubMed" id="22037399"/>
            <dbReference type="DOI" id="10.1128/JB.06026-11"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>GENE FAMILY</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Transpeptidase that recognizes and modifies its substrate by proteolytic cleavage of a sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the archaeosortase and its substrate, which is then transferred and covalently attached to the cell membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the exosortase/archaeosortase family. Archaeosortase E subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64303">
        <property type="entry name" value="C64303"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869519.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60336"/>
    <dbReference type="SMR" id="Q60336"/>
    <dbReference type="STRING" id="243232.MJ_0027"/>
    <dbReference type="TCDB" id="9.B.297.4.1">
        <property type="family name" value="the archaeosortase/exosortase/rhomosortase (sortase) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98013">
        <property type="protein sequence ID" value="AAB98013"/>
        <property type="gene ID" value="MJ_0027"/>
    </dbReference>
    <dbReference type="GeneID" id="1450865"/>
    <dbReference type="KEGG" id="mja:MJ_0027"/>
    <dbReference type="eggNOG" id="arCOG04471">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134207_0_0_2"/>
    <dbReference type="OMA" id="NGEYIHN"/>
    <dbReference type="OrthoDB" id="105606at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026485">
        <property type="entry name" value="Archaeo_ArtE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019127">
        <property type="entry name" value="Exosortase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09721">
        <property type="entry name" value="Exosortase_EpsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04124">
        <property type="entry name" value="archaeo_artE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106660" description="Probable archaeosortase E">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="90"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="D4GUZ4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="22037399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="22037399"/>
        </source>
    </evidence>
    <sequence length="184" mass="21259" checksum="DA95536BB790C5B4" modified="1997-11-01" version="1">MGSLLMERNFMVEDTFTNGKLSKKEKILFLIKFYIIFLVVFFILSYFGKYLIGIVTYLSYIFTKIIISDARLADNFIYLPNNTVEVVEECTGSFLIAGLLALIIVYSKNIKEFIIGIFFVLLAFFVNIFRIVLICYLVNMHPESSYLYHEIAGYGVILTLVPVLVIGYLKIIEKYRHSSNKSHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q60338</accession>
    <name>FRHA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Coenzyme F420 hydrogenase subunit alpha</fullName>
            <ecNumber>1.12.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha</fullName>
            <shortName>FRH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">frhA</name>
        <name type="ordered locus">MJ0029</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>H(+) + H2 + oxidized coenzyme F420-(gamma-L-Glu)(n) = reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:23760"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.12.98.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
        <text evidence="1">There are 12-13 Fe atoms/(alpha(1)beta(1)gamma(1)) unit of the FRH.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterocomplex of the form (alpha(1)beta(1)gamma(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64299">
        <property type="entry name" value="A64299"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60338"/>
    <dbReference type="SMR" id="Q60338"/>
    <dbReference type="InParanoid" id="Q60338"/>
    <dbReference type="OMA" id="HGIASCE"/>
    <dbReference type="PhylomeDB" id="Q60338"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017682">
        <property type="entry name" value="Coenz_F420_hydrogenase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03295">
        <property type="entry name" value="frhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00508">
        <property type="entry name" value="NI_HGENASE_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000199722" description="Coenzyme F420 hydrogenase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="415" mass="46784" checksum="8DD9A94CBABE35C4" modified="1997-11-01" version="1">MEVNFVTNRIEIAPTTRHEGHAKLILEVDEEGIVNKAYYLNTTPVRGFETMLKGKPAEFAPIAVMRICGICQTTHGIASCEAIENAIDCEVPDDGLLLRELVGIGNRLHSHPLHHLLTIDDFLKPDETDLKIELIKLIQRMRKVGQLVVDIVGGEGIHPPNIVIGGMRTNITERAKSRLYYALRQYEKDAYELYEKYTELIERYLEEIGIPDLGAHEYPYIATHTTYGDRYAINWDDVTEIPAQRYYDDEEAKQTTTIQIPLYAGVPAEGGPRARMVKFGNFREGGSAMDINIARAQENLGAVYRALEILDELDLNGKTRAEVEYKDGFGIGVHEAPRATNTHMAEVGKDGKIKSYRIIAASTWNFPIVEKAIEGYPQQYAEVIMRAYDICASCATHVIVKDEETKEIIEVRKML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q60366</accession>
    <name>AMT1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ammonium transporter Amt1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">amt1</name>
        <name type="ordered locus">MJ0058</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="EMBO J." volume="26" first="589" last="599">
            <title>Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake.</title>
            <authorList>
                <person name="Yildiz O."/>
                <person name="Kalthoff C."/>
                <person name="Raunser S."/>
                <person name="Kuhlbrandt W."/>
            </authorList>
            <dbReference type="PubMed" id="17203075"/>
            <dbReference type="DOI" id="10.1038/sj.emboj.7601492"/>
        </citation>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH GLNK1</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3)) (By similarity). Transport is electrogenic.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">Activity is regulated by the nitrogen regulatory protein GlnK1 via direct interaction (PubMed:17203075). Formation of the GlnK1/Amt1 complex is decreased in the presence of Mg-ATP or 2-oxoglutarate. The presence of both effectors abolishes the formation of the complex (PubMed:17203075).</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer (PubMed:17203075). Interacts and forms a complex with GlnK1 (PubMed:17203075).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64307">
        <property type="entry name" value="B64307"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60366"/>
    <dbReference type="SMR" id="Q60366"/>
    <dbReference type="STRING" id="243232.MJ_0058"/>
    <dbReference type="EnsemblBacteria" id="AAB98038">
        <property type="protein sequence ID" value="AAB98038"/>
        <property type="gene ID" value="MJ_0058"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0058"/>
    <dbReference type="eggNOG" id="arCOG04397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_33_1_2"/>
    <dbReference type="InParanoid" id="Q60366"/>
    <dbReference type="OMA" id="CAGSDVM"/>
    <dbReference type="PhylomeDB" id="Q60366"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072488">
        <property type="term" value="P:ammonium transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0924">Ammonia transport</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000139764" description="Ammonium transporter Amt1">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="261"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="305"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="site" description="Twin-His motif. Important for optimum substrate conductance" evidence="2">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="site" description="Twin-His motif. Important for optimum substrate conductance" evidence="2">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O29285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P69681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="17203075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="17203075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="391" mass="41153" checksum="33632A73849E1C9A" modified="1997-11-01" version="1">MFEVKHMDGIDVFFFMWAASLIFFMKAGFIALEIGQFRAKNVSYHCVLKLLDLAAVFIAYLFIGYGISYGFENIMPLITGTFDADLGAWWMKMVMFAAAAVTIITGGVAERIKILPYFIGALIVGGILYPIVEHLVWGGGFANLGINFHDYAGSGAVHLFGGLVGLMAAYVLGPRIDKYINGKPQAIPGHNIPIAVLGAFILAFGWYGFNIGSASGIANGVELASVAMATTMALAGGIIGGALSSRNDPLYTANGMCAGLVAVCSGVDLFTPIGAFIVGLLAGIQQPFTYKFIEEKLKIDDVCAIGPVHAMSGLIGVICAGIPFLLKADAVSKVSITGQIIGAIVIALIAIVGGLIIYKGLDLTIGLRVSEEAEKVGLDTAILQTTAYSEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q60373</accession>
    <name>RELB1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative antitoxin RelB1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">relB1</name>
        <name type="ordered locus">MJ0070</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is RelE1.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64308">
        <property type="entry name" value="F64308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869562.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60373"/>
    <dbReference type="SMR" id="Q60373"/>
    <dbReference type="STRING" id="243232.MJ_0070"/>
    <dbReference type="EnsemblBacteria" id="AAB98053">
        <property type="protein sequence ID" value="AAB98053"/>
        <property type="gene ID" value="MJ_0070"/>
    </dbReference>
    <dbReference type="GeneID" id="1450909"/>
    <dbReference type="KEGG" id="mja:MJ_0070"/>
    <dbReference type="eggNOG" id="arCOG09828">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2550326_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000106678" description="Putative antitoxin RelB1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="82" mass="9607" checksum="298230477B2C550B" modified="1997-11-01" version="1">MLNINKEIAQIETELNELKKLRDEISERIEKLEIKLLKLKALAIPEEEFEEDYEEIIEDVKKSLDKKETVPAEEALKELGLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q60375</accession>
    <name>Y063_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0063</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0063</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64307">
        <property type="entry name" value="G64307"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60375"/>
    <dbReference type="SMR" id="Q60375"/>
    <dbReference type="STRING" id="243232.MJ_0063"/>
    <dbReference type="EnsemblBacteria" id="AAB98050">
        <property type="protein sequence ID" value="AAB98050"/>
        <property type="gene ID" value="MJ_0063"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0063"/>
    <dbReference type="eggNOG" id="arCOG08289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_124805_0_0_2"/>
    <dbReference type="OMA" id="NCASSIP"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106674" description="Uncharacterized protein MJ0063">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <sequence length="181" mass="21173" checksum="E007B9432CB52053" modified="1997-11-01" version="1">MFQMENRNDELFIKLDSSIKSLLRSAREFKKENESISNVLLQLAEMLDNIDKTLEIIEKNFQIILKNRESGKFSNNEIIQKFVKPLENLIKVIENIESTSNNLKNEIENCASSIPTLKEITDKLKIINMESATQAIEEFKIAYDMLEDNRKNLDELIEKTKILKDKLKNLLLQIDNFLNEH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q60377</accession>
    <name>Y066_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0066</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0066</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the PAPS reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64308">
        <property type="entry name" value="B64308"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60377"/>
    <dbReference type="SMR" id="Q60377"/>
    <dbReference type="STRING" id="243232.MJ_0066"/>
    <dbReference type="EnsemblBacteria" id="AAB98046">
        <property type="protein sequence ID" value="AAB98046"/>
        <property type="gene ID" value="MJ_0066"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0066"/>
    <dbReference type="eggNOG" id="arCOG00073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026622_0_0_2"/>
    <dbReference type="InParanoid" id="Q60377"/>
    <dbReference type="OMA" id="RWCCKVT"/>
    <dbReference type="PhylomeDB" id="Q60377"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100693" description="Uncharacterized protein MJ0066">
        <location>
            <begin position="1"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="131"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="480" mass="56371" checksum="8076C1D9A79B53D3" modified="1997-11-01" version="1">MWEVIIMKTYIGKIHLKWCKNCNVPLLGRVCEVCGSKAEEVKLTPPGDPRLGFQYDMDFINKILEEEFGAKNVLNGKIILLNKIPGNEEAYEIIVDGEVKYLIYFDEDKEKWKVKLKLNGAKDLMEKGAYKKIIKIKNDVVEFLKNRKGSVLRPGIVEFTDDIEEKDDVIIVDENDRVVGVGLAVVSSEDIKNMEKGKVVKVRFFIKDNEDYKPGKIYDNLEEAFDLMVRANEGVIDNYERNAIGFIKNTYEKIKKPVMVAFSGGKDSLVTLILTLKALGKDIDVVFIDTGLEFEETLKNVEDVERHYGIKIIRLRGENFWEKVKEYGIPARDYRWCSEICKLEPLKKFIEENYEDDVLSFVGIRKYESFNRATKKRIHRNTYIKKQINALPIFHWSSLHVWIYLLREKAPYNKLYEKGFDRIGCFMCPAMEMGEMNKIKREFPKLWEKWENVLREYAEKHNLGEGWIKKGLWRWKHKRQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q60381</accession>
    <name>GLNK1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nitrogen regulatory protein GlnK1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">glnK1</name>
        <name type="ordered locus">MJ0059</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2007" name="EMBO J." volume="26" first="589" last="599">
            <title>Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake.</title>
            <authorList>
                <person name="Yildiz O."/>
                <person name="Kalthoff C."/>
                <person name="Raunser S."/>
                <person name="Kuhlbrandt W."/>
            </authorList>
            <dbReference type="PubMed" id="17203075"/>
            <dbReference type="DOI" id="10.1038/sj.emboj.7601492"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEXES WITH ADP; AMP; ATP; MAGNESIUM AND 2-OXOGLUTARATE</scope>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH AMT1</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the regulation of nitrogen metabolism (PubMed:17203075). Regulates the activity of its targets by protein-protein interaction in response to the nitrogen status of the cell (PubMed:17203075). Regulates the activity of the ammonia channel Amt1 via direct interaction (PubMed:17203075).</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Formation of the GlnK1/Amt1 complex is decreased in the presence of Mg-ATP or 2-oxoglutarate. The presence of both effectors abolishes the formation of the complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer (PubMed:17203075). Interacts and forms a complex with Amt1 (PubMed:17203075).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-7201321">
            <id>Q60381</id>
        </interactant>
        <interactant intactId="EBI-7201308">
            <id>Q58739</id>
            <label>amt2</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64307">
        <property type="entry name" value="C64307"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869551.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2J9C">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.30 A"/>
        <property type="chains" value="A/B/C=1-112"/>
    </dbReference>
    <dbReference type="PDB" id="2J9D">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-112"/>
    </dbReference>
    <dbReference type="PDB" id="2J9E">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.62 A"/>
        <property type="chains" value="A/B/C=1-112"/>
    </dbReference>
    <dbReference type="PDB" id="7P52">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.20 A"/>
        <property type="chains" value="A=1-112"/>
    </dbReference>
    <dbReference type="PDBsum" id="2J9C"/>
    <dbReference type="PDBsum" id="2J9D"/>
    <dbReference type="PDBsum" id="2J9E"/>
    <dbReference type="PDBsum" id="7P52"/>
    <dbReference type="AlphaFoldDB" id="Q60381"/>
    <dbReference type="SMR" id="Q60381"/>
    <dbReference type="IntAct" id="Q60381">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="MINT" id="Q60381"/>
    <dbReference type="STRING" id="243232.MJ_0059"/>
    <dbReference type="EnsemblBacteria" id="AAB98041">
        <property type="protein sequence ID" value="AAB98041"/>
        <property type="gene ID" value="MJ_0059"/>
    </dbReference>
    <dbReference type="GeneID" id="1450898"/>
    <dbReference type="KEGG" id="mja:MJ_0059"/>
    <dbReference type="eggNOG" id="arCOG02305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_2"/>
    <dbReference type="InParanoid" id="Q60381"/>
    <dbReference type="OMA" id="HQIEVNF"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="PhylomeDB" id="Q60381"/>
    <dbReference type="EvolutionaryTrace" id="Q60381"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039144">
        <property type="entry name" value="GlnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139815" description="Nitrogen regulatory protein GlnK1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 6 8">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 6 8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>2-oxoglutarate</name>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 7">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 6 8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 6 8">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 7">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 7">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 6 8">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="2"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="38"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="41"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="49"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="70"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="88"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="turn" evidence="11">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="B8ZYW0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="17203075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="17203075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="2J9C"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="2J9D"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="2J9E"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="2J9C"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="2J9D"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="7P52"/>
        </source>
    </evidence>
    <sequence length="112" mass="12490" checksum="3DC3F307C32FE9F3" modified="1997-11-01" version="1">MKKVEAIIRPEKLEIVKKALSDAGYVGMTVSEVKGRGVQGGIVERYRGREYIVDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKIFVIPVERVVRVRTKEEGKEAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q60386</accession>
    <name>Y080_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transcriptional regulator MJ0080</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0080</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64309">
        <property type="entry name" value="H64309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869572.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60386"/>
    <dbReference type="SMR" id="Q60386"/>
    <dbReference type="STRING" id="243232.MJ_0080"/>
    <dbReference type="EnsemblBacteria" id="AAB98060">
        <property type="protein sequence ID" value="AAB98060"/>
        <property type="gene ID" value="MJ_0080"/>
    </dbReference>
    <dbReference type="GeneID" id="1450919"/>
    <dbReference type="KEGG" id="mja:MJ_0080"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_1_2_2"/>
    <dbReference type="OMA" id="YDWLSRI"/>
    <dbReference type="OrthoDB" id="101089at2157"/>
    <dbReference type="PhylomeDB" id="Q60386"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000139318" description="Uncharacterized transcriptional regulator MJ0080">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="127" mass="14892" checksum="BB57018575CF9AE4" modified="1997-11-01" version="1">MERISLTIEKNLLKEVDEIINKERISRSEFIRRALEYYVKKYDWLSRIESKIGEITVIYNSKAVEDIVKLESQYKDIVIISLEIPFEGKIIRMIAIKGQRDRIIEFTNKLKGISSVELAQLTTISIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>P54025</accession>
    <name>RL41_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L41e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl41e</name>
        <name type="ordered locus">MJ0242</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL41 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98230" version="1"/>
        </conflict>
        <text>Truncated N-terminus.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAB98230" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98230.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64330">
        <property type="entry name" value="C64330"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54025"/>
    <dbReference type="SMR" id="P54025"/>
    <dbReference type="STRING" id="243232.MJ_0242"/>
    <dbReference type="EnsemblBacteria" id="AAB98230">
        <property type="protein sequence ID" value="AAB98230"/>
        <property type="gene ID" value="MJ_0242"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0242"/>
    <dbReference type="HOGENOM" id="CLU_220499_2_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000198075" description="50S ribosomal protein L41e">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="38" mass="5098" checksum="083830232FDB0B4D" modified="2017-06-07" version="2">MIPIKRSSRRWKKKGRMRWKWYKKRLRRLKRERKRARS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>P54031</accession>
    <name>RS27A_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S27ae</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps27ae</name>
        <name type="ordered locus">MJ0393</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64349">
        <property type="entry name" value="A64349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869892.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54031"/>
    <dbReference type="STRING" id="243232.MJ_0393"/>
    <dbReference type="EnsemblBacteria" id="AAB98383">
        <property type="protein sequence ID" value="AAB98383"/>
        <property type="gene ID" value="MJ_0393"/>
    </dbReference>
    <dbReference type="GeneID" id="1451250"/>
    <dbReference type="KEGG" id="mja:MJ_0393"/>
    <dbReference type="eggNOG" id="arCOG04183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_179743_2_0_2"/>
    <dbReference type="InParanoid" id="P54031"/>
    <dbReference type="OMA" id="GVFMAEH"/>
    <dbReference type="OrthoDB" id="123606at2157"/>
    <dbReference type="PhylomeDB" id="P54031"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_S27ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_S27a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_S27ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000137694" description="30S ribosomal protein S27ae">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <sequence length="60" mass="7048" checksum="796AA6E07CCA47F7" modified="1996-10-01" version="1">MTKGKKTAKYKYYKIEGDKVIRLKKTCPRCGPGVFMAEHLNRYACGKCGYMEWKQPQKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>P54035</accession>
    <name>RL29_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl29</name>
        <name type="ordered locus">MJ0462</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64357">
        <property type="entry name" value="F64357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869962.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54035"/>
    <dbReference type="SMR" id="P54035"/>
    <dbReference type="STRING" id="243232.MJ_0462"/>
    <dbReference type="EnsemblBacteria" id="AAB98451">
        <property type="protein sequence ID" value="AAB98451"/>
        <property type="gene ID" value="MJ_0462"/>
    </dbReference>
    <dbReference type="GeneID" id="1451324"/>
    <dbReference type="KEGG" id="mja:MJ_0462"/>
    <dbReference type="eggNOG" id="arCOG00785">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158491_2_2_2"/>
    <dbReference type="InParanoid" id="P54035"/>
    <dbReference type="OMA" id="ELRDMTP"/>
    <dbReference type="OrthoDB" id="125807at2157"/>
    <dbReference type="PhylomeDB" id="P54035"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_L29/L35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_L29/L35_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_L29_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="SSF46561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000130511" description="50S ribosomal protein L29">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="70" mass="8082" checksum="95FC2D0736AF500A" modified="1996-10-01" version="1">MAILRADELRGMSMEELKEKLVELKRELLKERASKAVAGAPSNPGRMREIRRTIARILTIMNEKKRMTSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2019-12-11" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>P61154</accession>
    <name>FWDB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tungsten-containing formylmethanofuran dehydrogenase 2 subunit B</fullName>
            <ecNumber evidence="2">1.2.7.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Tungsten-containing formylmethanofuran dehydrogenase II subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">fwdB</name>
        <name type="ordered locus">MJ1194</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="J. Mol. Biol." volume="266" first="637" last="641">
            <title>Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion.</title>
            <authorList>
                <person name="Wilting R."/>
                <person name="Schorling S."/>
                <person name="Persson B.C."/>
                <person name="Boeck A."/>
            </authorList>
            <dbReference type="PubMed" id="9102456"/>
            <dbReference type="DOI" id="10.1006/jmbi.1996.0812"/>
        </citation>
        <scope>PROBABLE SELENOCYSTEINE AT SEC-121</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + methanofuran + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>W-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60537"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">This enzyme is composed of six subunits FwdA, FwdC, FwdD, FwdE, FwdF and FwdG.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FwdB family.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="P61154"/>
    <dbReference type="OMA" id="VCIDPHE"/>
    <dbReference type="PhylomeDB" id="P61154"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd02761">
        <property type="entry name" value="MopB_FmdB-FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016457">
        <property type="entry name" value="Formylmethanofuran_DH_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005646">
        <property type="entry name" value="FwdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03129">
        <property type="entry name" value="one_C_dehyd_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <keyword id="KW-0826">Tungsten</keyword>
    <feature type="chain" id="PRO_0000087392" description="Tungsten-containing formylmethanofuran dehydrogenase 2 subunit B">
        <location>
            <begin position="1"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="3">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q48943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="435" mass="48718" checksum="C1652D23FFC20970" modified="2008-02-26" version="2">MVKVVRNVVCPFCGTLCDDLEILVEDNHIVGTRHACRIGNAKFMHFEGAVRYTEPLMRENKKDDFKKVDYETAIEETARLLTEATLPLIYGWSATECHAHMYGVELAELVGAVIDNTASVUHGPSLLAVQDVGYPVCTLGEVKNRADVIIFWGSNPMHAHPRHMSRYSVFARGFFRERGREDRTLIVVDPRETDTAKLADIHLQVEPHKDYELVSAMRAVLKGFELQVDKVAGVPADLIYEAVEVCKNAQFGELFFAMGVTMTRGKHRNIDNAIQLVIDLNAYTKFGLMPMRGHYNVNGFNQVLTWVTGYPFGVDFSRGYPRYNPGETTANDLLQRGETDMMLNIASDPGAHFPQKAVQHMAKIPLVCIDPHETPTTQLANIIIPPAIAGVEVEGTAYRMDGVPIQLRKVIDPPEGVLPDREILKILIKKVKEML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="63" xmlns="http://uniprot.org/uniprot">
    <accession>P81235</accession>
    <name>YF1B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1516.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1516.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81235"/>
    <dbReference type="STRING" id="243232.MJ_1516.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99537">
        <property type="protein sequence ID" value="AAB99537"/>
        <property type="gene ID" value="MJ_1516.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1516.1"/>
    <dbReference type="HOGENOM" id="CLU_1691556_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107388" description="Uncharacterized protein MJ1516.1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <sequence length="155" mass="17259" checksum="95AFCE3E1A99C417" modified="1998-06-01" version="1">MTRSSSVDVVVRHFFLSLNIRRGRITGTPMIPYNGHNGAIEYNTNVAKNQQKILNIIFRNPILPPNIQSFKKSNSIPNIVKTINNANTVTTSANTPIPTSPKLEEINPSIIIPIHKTENIIPPIIKKIHAIRNIITITLIMSSIKSNIIKSPPKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>P81312</accession>
    <name>Y78A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0782.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0782.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH886.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870288.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81312"/>
    <dbReference type="SMR" id="P81312"/>
    <dbReference type="STRING" id="243232.MJ_0782.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98772">
        <property type="protein sequence ID" value="AAB98772"/>
        <property type="gene ID" value="MJ_0782.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451660"/>
    <dbReference type="KEGG" id="mja:MJ_0782.1"/>
    <dbReference type="eggNOG" id="arCOG02466">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165884_4_0_2"/>
    <dbReference type="OMA" id="FVMNEQP"/>
    <dbReference type="OrthoDB" id="115856at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107032" description="Uncharacterized protein MJ0782.1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="81" mass="9169" checksum="3B1B0ED0220433AC" modified="1998-07-15" version="1">MKVEILHKTPKGFLIARGKREIKIGSVVIFKNKKIGKVVDIFGPVAKPYIKILPINKDIEVSGTAYIKNDKSKYKNTEKKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>P81326</accession>
    <name>T2M6_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative type II restriction enzyme MjaVIP</fullName>
            <shortName>R.MjaVIP</shortName>
            <ecNumber>3.1.21.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Endonuclease MjaVIP</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Type-2 restriction enzyme MjaVIP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaVIRP</name>
        <name type="ordered locus">MJ1208.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A P subtype restriction enzyme that recognizes the double-stranded sequence 5'-CCGG-3'; the cleavage site is unknown.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.21.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BsaWI type II restriction endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870721.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81326"/>
    <dbReference type="REBASE" id="16564">
        <property type="entry name" value="MjaVIP"/>
    </dbReference>
    <dbReference type="PRIDE" id="P81326"/>
    <dbReference type="EnsemblBacteria" id="AAB99221">
        <property type="protein sequence ID" value="AAB99221"/>
        <property type="gene ID" value="MJ_1208.1"/>
    </dbReference>
    <dbReference type="GeneID" id="27929990"/>
    <dbReference type="KEGG" id="mja:MJ_1208.1"/>
    <dbReference type="eggNOG" id="arCOG00115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095647_0_0_2"/>
    <dbReference type="InParanoid" id="P81326"/>
    <dbReference type="OMA" id="AKCINGE"/>
    <dbReference type="OrthoDB" id="133558at2157"/>
    <dbReference type="PhylomeDB" id="P81326"/>
    <dbReference type="PRO" id="PR:P81326"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009036">
        <property type="term" value="F:type II site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041551">
        <property type="entry name" value="RE_BsaWI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18643">
        <property type="entry name" value="RE_BsaWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000077337" description="Putative type II restriction enzyme MjaVIP">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="226" mass="26711" checksum="9A7B306A28A6A2F2" modified="1998-06-01" version="1">MLHFGGFIMEINHISKILEKEREEYIRNKVEEYLKQGFSKDDAVNKANQSWRTYIGHRIQDVIYNLLKKFLKDSGLKVTTDKALNNRNLPEELDKVKRLIAINYGEYLFLPDADVIVYKVENNDIKIIAIISVKNSFRERRFETTYWKLKLKESPVTSHIKVFLATPDKDNEISYKCPNGKPKKMRIILEYELDGIYFLKEDFEETEKAKHFGKIVEDIIEISKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>P81335</accession>
    <name>VHUU_METJA</name>
    <protein>
        <recommendedName>
            <fullName>F420-non-reducing hydrogenase vhu subunit U</fullName>
            <ecNumber>1.12.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vhuU</name>
        <name type="ordered locus">MJ1192.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="J. Mol. Biol." volume="266" first="637" last="641">
            <title>Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion.</title>
            <authorList>
                <person name="Wilting R."/>
                <person name="Schorling S."/>
                <person name="Persson B.C."/>
                <person name="Boeck A."/>
            </authorList>
            <dbReference type="PubMed" id="9102456"/>
            <dbReference type="DOI" id="10.1006/jmbi.1996.0812"/>
        </citation>
        <scope>PROBABLE SELENOCYSTEINE AT SEC-27</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">The F420-non-reducing hydrogenase vhu is composed of four subunits; VhuA, VhuD, VhuG and VhuU.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The large subunit of Vhu is split into VhuA and VhuU. Each contributes two ligands to the [NiFeSe] center.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous termination">
            <sequence resource="EMBL-CDS" id="AAB99197" version="1"/>
        </conflict>
        <text>Truncated C-terminus.</text>
    </comment>
    <dbReference type="EC" id="1.12.99.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99197.1"/>
        <property type="status" value="ALT_SEQ"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1192.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99197">
        <property type="protein sequence ID" value="AAB99197"/>
        <property type="gene ID" value="MJ_1192.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1192.1"/>
    <dbReference type="HOGENOM" id="CLU_3387472_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <feature type="chain" id="PRO_0000013409" description="F420-non-reducing hydrogenase vhu subunit U">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="propeptide" id="PRO_0000013410" description="Removed in mature form" evidence="1">
        <location>
            <begin position="34"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="3">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="50" mass="5726" checksum="A0BF6051D99602A0" modified="2008-02-26" version="3" precursor="true">MAEKSTVKVDEVKLNLIEMVLRAYDPUYSCAAHIIVKDEKGNKIIEVIKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q57599</accession>
    <name>RNH2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Ribonuclease HII</fullName>
            <shortName>RNase HII</shortName>
            <ecNumber>3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rnhB</name>
        <name type="ordered locus">MJ0135</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Biochemistry" volume="42" first="785" last="791">
            <title>Metal ion binding and enzymatic mechanism of Methanococcus jannaschii RNase HII.</title>
            <authorList>
                <person name="Lai B."/>
                <person name="Li Y."/>
                <person name="Cao A."/>
                <person name="Lai L."/>
            </authorList>
            <dbReference type="PubMed" id="12534291"/>
            <dbReference type="DOI" id="10.1021/bi026960a"/>
        </citation>
        <scope>COFACTOR</scope>
        <scope>MUTAGENESIS OF ASP-7; GLU-8; ASP-112 AND ASP-149</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="Structure" volume="8" first="897" last="904">
            <title>Crystal structure of archaeal RNase HII: a homologue of human major RNase H.</title>
            <authorList>
                <person name="Lai L."/>
                <person name="Yokota H."/>
                <person name="Hung L.-W."/>
                <person name="Kim R."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="10997908"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(00)00179-9"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text>Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNase HII family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64316">
        <property type="entry name" value="G64316"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869628.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1EKE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=1-230"/>
    </dbReference>
    <dbReference type="PDBsum" id="1EKE"/>
    <dbReference type="AlphaFoldDB" id="Q57599"/>
    <dbReference type="SMR" id="Q57599"/>
    <dbReference type="STRING" id="243232.MJ_0135"/>
    <dbReference type="PRIDE" id="Q57599"/>
    <dbReference type="EnsemblBacteria" id="AAB98116">
        <property type="protein sequence ID" value="AAB98116"/>
        <property type="gene ID" value="MJ_0135"/>
    </dbReference>
    <dbReference type="GeneID" id="1450976"/>
    <dbReference type="KEGG" id="mja:MJ_0135"/>
    <dbReference type="eggNOG" id="arCOG04121">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036532_0_4_2"/>
    <dbReference type="InParanoid" id="Q57599"/>
    <dbReference type="OMA" id="REECRFF"/>
    <dbReference type="OrthoDB" id="105421at2157"/>
    <dbReference type="PhylomeDB" id="Q57599"/>
    <dbReference type="EvolutionaryTrace" id="Q57599"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00052_A">
        <property type="entry name" value="RNase_HII_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020787">
        <property type="entry name" value="RNase_HII_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="PTHR10954"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00729">
        <property type="entry name" value="TIGR00729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000111663" description="Ribonuclease HII">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="RNase H type-2" evidence="2">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 800-fold." evidence="3">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 700-fold." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 600-fold." evidence="3">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 800-fold." evidence="3">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="149"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="2"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="18"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="27"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="30"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="60"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="69"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="104"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="139"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="148"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="153"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="213"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12534291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1EKE"/>
        </source>
    </evidence>
    <sequence length="230" mass="26506" checksum="C454324C0C801299" modified="1997-11-01" version="1">MIIIGIDEAGRGPVLGPMVVCAFAIEKEREEELKKLGVKDSKELTKNKRAYLKKLLENLGYVEKRILEAEEINQLMNSINLNDIEINAFSKVAKNLIEKLNIRDDEIEIYIDACSTNTKKFEDSFKDKIEDIIKERNLNIKIIAEHKADAKYPVVSAASIIAKAERDEIIDYYKKIYGDIGSGYPSDPKTIKFLEDYFKKHKKLPDIARTHWKTCKRILDKSKQTKLIIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q57648</accession>
    <name>RPO3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit D</fullName>
            <shortName evidence="3">mjD</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo3</name>
        <name evidence="1" type="synonym">rpoD</name>
        <name type="ordered locus">MJ0192</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Nucleic Acids Res." volume="28" first="4299" last="4305">
            <title>Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12.</title>
            <authorList>
                <person name="Werner F."/>
                <person name="Eloranta J.J."/>
                <person name="Weinzierl R.O."/>
            </authorList>
            <dbReference type="PubMed" id="11058130"/>
            <dbReference type="DOI" id="10.1093/nar/28.21.4299"/>
        </citation>
        <scope>INTERACTION WITH RPO12</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the RNA polymerase complex (By similarity). Interacts with Rpo12. Forms an Rpo3-Rpo10-Rpo11-Rpo12 complex upon coexpression (PubMed:11058130).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-2567023">
            <id>Q57648</id>
        </interactant>
        <interactant intactId="EBI-2567038">
            <id>Q57832</id>
            <label>rpo11</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64324">
        <property type="entry name" value="A64324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869687.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57648"/>
    <dbReference type="SMR" id="Q57648"/>
    <dbReference type="IntAct" id="Q57648">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0192"/>
    <dbReference type="EnsemblBacteria" id="AAB98172">
        <property type="protein sequence ID" value="AAB98172"/>
        <property type="gene ID" value="MJ_0192"/>
    </dbReference>
    <dbReference type="GeneID" id="1451040"/>
    <dbReference type="KEGG" id="mja:MJ_0192"/>
    <dbReference type="eggNOG" id="arCOG04241">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_3_1_2"/>
    <dbReference type="InParanoid" id="Q57648"/>
    <dbReference type="OMA" id="AKWQPCN"/>
    <dbReference type="OrthoDB" id="72726at2157"/>
    <dbReference type="PhylomeDB" id="Q57648"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="SSF55257"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="SSF56553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000132754" description="DNA-directed RNA polymerase subunit Rpo3">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <sequence length="191" mass="21699" checksum="3F5B96CA0D242A96" modified="1997-11-01" version="1">MITIKEKRKTRIGEEFIFSLKAPISFSNAIRRIMISEVPTFAIEDVYIYENSSSMDDEILAHRLGLIPIKGKPLLENEVITFTLEKEGPCTVYSSDLKSENGEVAFKNIPIVKLGKGQRIQIECEAIPGIGKVHAKWQPCNAVYKQIADDEVEFFVETFGQMEAEEILEEAVKILKNKAESFLQQLEMIEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q57653</accession>
    <name>Y200_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized hydrogenase expression/formation protein MJ0200</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0200</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64325">
        <property type="entry name" value="A64325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869695.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57653"/>
    <dbReference type="SMR" id="Q57653"/>
    <dbReference type="STRING" id="243232.MJ_0200"/>
    <dbReference type="EnsemblBacteria" id="AAB98184">
        <property type="protein sequence ID" value="AAB98184"/>
        <property type="gene ID" value="MJ_0200"/>
    </dbReference>
    <dbReference type="GeneID" id="24892240"/>
    <dbReference type="KEGG" id="mja:MJ_0200"/>
    <dbReference type="eggNOG" id="arCOG04427">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_159381_2_2_2"/>
    <dbReference type="InParanoid" id="Q57653"/>
    <dbReference type="OMA" id="MCLAIPC"/>
    <dbReference type="OrthoDB" id="119258at2157"/>
    <dbReference type="PhylomeDB" id="Q57653"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902670">
        <property type="term" value="F:carbon dioxide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019812">
        <property type="entry name" value="Hydgase_assmbl_chp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="PTHR35177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01097">
        <property type="entry name" value="HUPF_HYPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000201408" description="Uncharacterized hydrogenase expression/formation protein MJ0200">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="82" mass="9348" checksum="46C7C47A06FAB808" modified="1997-11-01" version="1">MCLAIPCKVVEIIEEDGEKYAIAEYKGVKQKAKLTLLDKEVKIGDYILIHTGYALEVLSEEDAKLSLEAWEELFKALEEMEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q57662</accession>
    <name>PPS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="1">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phosphopantothenate synthetase</fullName>
            <shortName evidence="1">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0209</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-4-phosphopantoate + ATP + beta-alanine = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64326">
        <property type="entry name" value="B64326"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57662"/>
    <dbReference type="SMR" id="Q57662"/>
    <dbReference type="STRING" id="243232.MJ_0209"/>
    <dbReference type="PRIDE" id="Q57662"/>
    <dbReference type="EnsemblBacteria" id="AAB98192">
        <property type="protein sequence ID" value="AAB98192"/>
        <property type="gene ID" value="MJ_0209"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0209"/>
    <dbReference type="eggNOG" id="arCOG04262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078701_0_0_2"/>
    <dbReference type="InParanoid" id="Q57662"/>
    <dbReference type="OMA" id="PKSHPRY"/>
    <dbReference type="PhylomeDB" id="Q57662"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="PTHR40695"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106742" description="4-phosphopantoate--beta-alanine ligase">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="193"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="199"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <sequence length="266" mass="29853" checksum="D3B064215551E6C5" modified="1996-11-01" version="1">MLSVMRMQIPKTHPRYESLMKREKIIEALDKGILAKAGLIAHGRGETFDYLIGEKTAPIALEAIKAAAALLILAENPVISVNGNTVALAIDEVVELAKELNGKIEVNLFYRTKERELAIKRAFEEKFKDDIETGKIKILGIDDANKQIPNLDSLRGKVSEEGIFTADVVLVPLEDGDRAEALVNMGKKVIAIDLNPLSRTARKSTITIVDELTRAMPLLIKYVKEFKNKDREELLKIVEDFDNKKNLKDMIDYIAERLKNLSLDEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57682</accession>
    <name>Y229_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0229</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0229</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64328">
        <property type="entry name" value="F64328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869727.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57682"/>
    <dbReference type="SMR" id="Q57682"/>
    <dbReference type="STRING" id="243232.MJ_0229"/>
    <dbReference type="EnsemblBacteria" id="AAB98214">
        <property type="protein sequence ID" value="AAB98214"/>
        <property type="gene ID" value="MJ_0229"/>
    </dbReference>
    <dbReference type="GeneID" id="1451082"/>
    <dbReference type="KEGG" id="mja:MJ_0229"/>
    <dbReference type="eggNOG" id="arCOG01679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_9_1_2"/>
    <dbReference type="InParanoid" id="Q57682"/>
    <dbReference type="OMA" id="WYVYELA"/>
    <dbReference type="OrthoDB" id="111804at2157"/>
    <dbReference type="PhylomeDB" id="Q57682"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000160631" description="Uncharacterized HTH-type transcriptional regulator MJ0229">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <sequence length="95" mass="11180" checksum="FF6B8F2CD9D7488D" modified="1996-11-01" version="1">MKLIDVVKMGEALSNPIRVKILYILNKQPKNIYELAKELELSRPVVYAHLRKLEDADLVESDLVLEGSRAKRIYKAKEFKFYIDNEIIKKLFENE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q57694</accession>
    <name>GATC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable glutamyl-tRNA(Gln) amidotransferase subunit C</fullName>
            <shortName>Glu-ADT subunit C</shortName>
            <ecNumber>6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0243</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-aspartyl-tRNA(Asn) + L-glutamine = ADP + 2 H(+) + L-asparaginyl-tRNA(Asn) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14513"/>
            <dbReference type="Rhea" id="RHEA-COMP:9674"/>
            <dbReference type="Rhea" id="RHEA-COMP:9677"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78515"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64330">
        <property type="entry name" value="D64330"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869741.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57694"/>
    <dbReference type="SMR" id="Q57694"/>
    <dbReference type="STRING" id="243232.MJ_0243"/>
    <dbReference type="EnsemblBacteria" id="AAB98231">
        <property type="protein sequence ID" value="AAB98231"/>
        <property type="gene ID" value="MJ_0243"/>
    </dbReference>
    <dbReference type="GeneID" id="1451097"/>
    <dbReference type="KEGG" id="mja:MJ_0243"/>
    <dbReference type="eggNOG" id="arCOG02726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196465_0_0_2"/>
    <dbReference type="OMA" id="MEESYYI"/>
    <dbReference type="OrthoDB" id="117523at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050566">
        <property type="term" value="F:asparaginyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="Asp/Glu-ADT_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010120">
        <property type="entry name" value="Glu-ADT_subunit_C_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="Glu-tRNAGln"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="SSF141000"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01827">
        <property type="entry name" value="gatC_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000105359" description="Probable glutamyl-tRNA(Gln) amidotransferase subunit C">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="82" mass="9786" checksum="D400012E5D3C201B" modified="1996-11-01" version="1">MDEKTIEKIKKEAEEIINKFSEVLEKFNLEMEESYYIIDTRNVLREDEAVESNPEFREKFLKIAPKVNKEGYVVVEKGSWLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q57703</accession>
    <name>PSLS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphosulfolactate synthase</fullName>
            <ecNumber>4.4.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>(2R)-phospho-3-sulfolactate synthase</fullName>
            <shortName>PSL synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">comA</name>
        <name type="ordered locus">MJ0255</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="13421" last="13429">
            <title>Identification of coenzyme M biosynthetic phosphosulfolactate synthase: a new family of sulfonate-biosynthesizing enzymes.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="11830598"/>
            <dbReference type="DOI" id="10.1074/jbc.M201011200"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>MUTAGENESIS OF LYS-137</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Catalyzes the addition of sulfite to phosphoenolpyruvate (PEP) to yield (2R)-phospho-3-sulfolactate (PSL).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R)-O-phospho-3-sulfolactate = H(+) + phosphoenolpyruvate + sulfite</text>
            <dbReference type="Rhea" id="RHEA:22784"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.4.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 8.5.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The enzyme is stereospecific.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the phosphosulfolactate synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.19"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64331">
        <property type="entry name" value="H64331"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869753.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1QWG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-251"/>
    </dbReference>
    <dbReference type="PDBsum" id="1QWG"/>
    <dbReference type="AlphaFoldDB" id="Q57703"/>
    <dbReference type="SMR" id="Q57703"/>
    <dbReference type="STRING" id="243232.MJ_0255"/>
    <dbReference type="EnsemblBacteria" id="AAB98242">
        <property type="protein sequence ID" value="AAB98242"/>
        <property type="gene ID" value="MJ_0255"/>
    </dbReference>
    <dbReference type="GeneID" id="1451109"/>
    <dbReference type="KEGG" id="mja:MJ_0255"/>
    <dbReference type="eggNOG" id="arCOG04896">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062679_2_0_2"/>
    <dbReference type="InParanoid" id="Q57703"/>
    <dbReference type="OMA" id="MFEASNA"/>
    <dbReference type="OrthoDB" id="37705at2157"/>
    <dbReference type="PhylomeDB" id="Q57703"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-2262"/>
    <dbReference type="BRENDA" id="4.4.1.19">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q57703"/>
    <dbReference type="UniPathway" id="UPA00355">
        <property type="reaction ID" value="UER00469"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57703"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043817">
        <property type="term" value="F:phosphosulfolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022370">
        <property type="entry name" value="Arch_ComA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003830">
        <property type="entry name" value="ComA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036112">
        <property type="entry name" value="ComA_synth_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02679">
        <property type="entry name" value="ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102110">
        <property type="entry name" value="SSF102110"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03849">
        <property type="entry name" value="arch_ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000080830" description="Phosphosulfolactate synthase">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of function." evidence="1">
        <original>K</original>
        <variation>N</variation>
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="5"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="16"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="66"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="70"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="75"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="107"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="129"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="163"/>
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        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="170"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="187"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="199"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="202"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="225"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="230"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="233"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="245"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="248"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11830598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1QWG"/>
        </source>
    </evidence>
    <sequence length="251" mass="28368" checksum="9B3F2CD349880E4C" modified="1996-11-01" version="1">MKAFEFLYEDFQRGLTVVLDKGLPPKFVEDYLKVCGDYIDFVKFGWGTSAVIDRDVVKEKINYYKDWGIKVYPGGTLFEYAYSKGKFDEFLNECEKLGFEAVEISDGSSDISLEERKNAIKRAKDNGFMVLTEVGKKMPDKDKQLTIDDRIKLINFDLDAGADYVIIEGRESGKGIGLFDKEGKVKENELDVLAKNVDINKVIFEAPQKSQQVAFILKFGSSVNLANIAFDEVISLETLRRGLRGDTFGKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57705</accession>
    <name>TYW1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="1">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>MjTYW1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">taw1</name>
        <name type="ordered locus">MJ0257</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="Mol. Biol. Evol." volume="27" first="2062" last="2077">
            <title>Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea.</title>
            <authorList>
                <person name="de Crecy-Lagard V."/>
                <person name="Brochier-Armanet C."/>
                <person name="Urbonavicius J."/>
                <person name="Fernandez B."/>
                <person name="Phillips G."/>
                <person name="Lyons B."/>
                <person name="Noma A."/>
                <person name="Alvarez S."/>
                <person name="Droogmans L."/>
                <person name="Armengaud J."/>
                <person name="Grosjean H."/>
            </authorList>
            <dbReference type="PubMed" id="20382657"/>
            <dbReference type="DOI" id="10.1093/molbev/msq096"/>
        </citation>
        <scope>GENE NAME</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="Biochemistry" volume="50" first="10573" last="10575">
            <title>Pyruvate is the source of the two carbons that are required for formation of the imidazoline ring of 4-demethylwyosine.</title>
            <authorList>
                <person name="Young A.P."/>
                <person name="Bandarian V."/>
            </authorList>
            <dbReference type="PubMed" id="22026549"/>
            <dbReference type="DOI" id="10.1021/bi2015053"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="J. Mol. Biol." volume="372" first="1204" last="1214">
            <title>Crystal structure of the radical SAM enzyme catalyzing tricyclic modified base formation in tRNA.</title>
            <authorList>
                <person name="Suzuki Y."/>
                <person name="Noma A."/>
                <person name="Suzuki T."/>
                <person name="Senda M."/>
                <person name="Senda T."/>
                <person name="Ishitani R."/>
                <person name="Nureki O."/>
            </authorList>
            <dbReference type="PubMed" id="17727881"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2007.07.024"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS)</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 4">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + CO2 + H2O + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TYW1 family.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64332">
        <property type="entry name" value="B64332"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869754.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2Z2U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=1-311"/>
    </dbReference>
    <dbReference type="PDB" id="6DJT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.64 A"/>
        <property type="chains" value="A=1-311"/>
    </dbReference>
    <dbReference type="PDBsum" id="2Z2U"/>
    <dbReference type="PDBsum" id="6DJT"/>
    <dbReference type="AlphaFoldDB" id="Q57705"/>
    <dbReference type="SMR" id="Q57705"/>
    <dbReference type="STRING" id="243232.MJ_0257"/>
    <dbReference type="EnsemblBacteria" id="AAB98244">
        <property type="protein sequence ID" value="AAB98244"/>
        <property type="gene ID" value="MJ_0257"/>
    </dbReference>
    <dbReference type="GeneID" id="1451111"/>
    <dbReference type="KEGG" id="mja:MJ_0257"/>
    <dbReference type="eggNOG" id="arCOG04174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007952_3_0_2"/>
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    <dbReference type="OMA" id="HRCLQMT"/>
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
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            <begin position="1"/>
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    <evidence type="ECO:0000255" key="1">
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    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
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        <source>
            <dbReference type="PubMed" id="17727881"/>
        </source>
    </evidence>
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            <dbReference type="PubMed" id="22026549"/>
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    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="6DJT"/>
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    </evidence>
    <sequence length="311" mass="36712" checksum="7B05311C25A29F3D" modified="1996-11-01" version="1">MIPEEIYKILRKQRYQIDGHTAVKLCGWVRKKMLEDKNCYKSKFYGIETHRCIQCTPSVIWCQQNCIFCWRVLPRDIGIDISQIKEPKWEEPEVVYEKILAMHKRIIMGYAGVLDRVGEKKFKEALEPKHVAISLSGEPTLYPYLDELIKIFHKNGFTTFVVSNGILTDVIEKIEPTQLYISLDAYDLDSYRRICGGKKEYWESILNTLDILKEKKRTCIRTTLIRGYNDDILKFVELYERADVHFIELKSYMHVGYSQKRLKKEDMLQHDEILKLAKMLDENSSYKLIDDSEDSRVALLQNENRKINPKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57716</accession>
    <name>PORD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Pyruvate synthase subunit PorD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Pyruvate oxidoreductase delta chain</fullName>
            <shortName>POR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Pyruvic-ferredoxin oxidoreductase subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">porD</name>
        <name type="ordered locus">MJ0268</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <comment type="subunit">
        <text>Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB98255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="E64333"/>
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        <property type="protein sequence ID" value="AAB98255"/>
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    <dbReference type="GeneID" id="1451122"/>
    <dbReference type="KEGG" id="mja:MJ_0268"/>
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        <property type="taxonomic scope" value="Archaea"/>
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        <property type="component" value="Chromosome"/>
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        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword id="KW-0249">Electron transport</keyword>
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    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
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            <begin position="25"/>
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        <source>
            <dbReference type="UniProtKB" id="P94692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="86" mass="9848" checksum="6F63454278C15091" modified="1996-11-01" version="1">MVTIAAIIYEPGNSIKNKTGTWRTFRPILDNEKCVKCENCYIFCPEGAIQEDENGNFKIDYDYCKGCLICMNECPVNAITKVREEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57721</accession>
    <name>Y273_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0273</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0273</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64334">
        <property type="entry name" value="B64334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57721"/>
    <dbReference type="SMR" id="Q57721"/>
    <dbReference type="STRING" id="243232.MJ_0273"/>
    <dbReference type="EnsemblBacteria" id="AAB98261">
        <property type="protein sequence ID" value="AAB98261"/>
        <property type="gene ID" value="MJ_0273"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0273"/>
    <dbReference type="eggNOG" id="arCOG01224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_180506_0_0_2"/>
    <dbReference type="InParanoid" id="Q57721"/>
    <dbReference type="OMA" id="GHLFTVE"/>
    <dbReference type="PhylomeDB" id="Q57721"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="SSF158372"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106765" description="Uncharacterized protein MJ0273">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <sequence length="103" mass="11972" checksum="BDE81755780AF7EB" modified="1996-11-01" version="1">MMVRNVIKEITEEKLENYFKRTEEAIKIIKKGMPPKRSLLYDVAKDFLLMIESYFEDAKAFKERGDYVTAFASLNYAYGWIDAGVRLGIFDVGDDDVRFTLAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57749</accession>
    <name>DHPSL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>7,8-dihydropterin-6-methyl-4-(beta-D-ribofuranosyl)-aminobenzene-5'-phosphate synthase</fullName>
            <ecNumber>2.5.1.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0301</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="Nat. Struct. Biol." volume="6" first="750" last="754">
            <title>Identifying two ancient enzymes in Archaea using predicted secondary structure alignment.</title>
            <authorList>
                <person name="Xu H."/>
                <person name="Aurora R."/>
                <person name="Rose G.D."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10426953"/>
            <dbReference type="DOI" id="10.1038/11525"/>
        </citation>
        <scope>FUNCTION AS A PTEROATE SYNTHASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of 6-hydroxymethyl-7,8-dihydropterin pyrophosphate (DHPP) with 4-(beta-D-ribofuranosyl)-aminobenzene-5'-phosphate (beta-RFA-P) to form 7,8-dihydropterin-6-methyl-4-(beta-D-ribofuranosyl)-aminobenzene-5'-phosphate, a precursor in the biosynthesis of 5,6,7,8-tetrahydromethanopterin (H4MPT). To a lesser extent, is able to condense beta-RFA-P with another arylamine, 1-(4-aminophenyl)-1-deoxy-D-ribitol (APDR), to form 7,8-dihydropterin-6-methyl-1-(4-aminophenyl)-1-deoxy-D-ribitol. Dephosphorylated beta-RFA-P is not a substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(7,8-dihydropterin-6-yl)methyl diphosphate + 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate = diphosphate + N-[(7,8-dihydropterin-6-yl)methyl]-4-(beta-D-ribofuranosyl)aniline 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:35951"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:72778"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:72951"/>
            <dbReference type="EC" id="2.5.1.105"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.105"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64337">
        <property type="entry name" value="F64337"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869799.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57749"/>
    <dbReference type="SMR" id="Q57749"/>
    <dbReference type="STRING" id="243232.MJ_0301"/>
    <dbReference type="EnsemblBacteria" id="AAB98288">
        <property type="protein sequence ID" value="AAB98288"/>
        <property type="gene ID" value="MJ_0301"/>
    </dbReference>
    <dbReference type="GeneID" id="1451156"/>
    <dbReference type="KEGG" id="mja:MJ_0301"/>
    <dbReference type="eggNOG" id="arCOG00503">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036012_0_0_2"/>
    <dbReference type="InParanoid" id="Q57749"/>
    <dbReference type="OMA" id="CSGWRAK"/>
    <dbReference type="OrthoDB" id="73329at2157"/>
    <dbReference type="PhylomeDB" id="Q57749"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14570"/>
    <dbReference type="BRENDA" id="2.5.1.105">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102041">
        <property type="term" value="F:7,8-dihydropterin-6-yl-methyl-4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102638">
        <property type="term" value="F:[1-(2-amino-7-methyl-4-oxo-7,8-dihydro-3H-pteridin-6-yl)]ethyl-4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901285">
        <property type="term" value="P:5,6,7,8-tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106783" description="7,8-dihydropterin-6-methyl-4-(beta-D-ribofuranosyl)-aminobenzene-5'-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10426953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="294" mass="33076" checksum="0FC92953FF9E005A" modified="1996-11-01" version="1">MLMNIGKVDNIKIYTLAEDYAGYNSPFWSQHGLSFLIEVESNGIKKRILFDTATYAEPILFNMKLLNINPKSIDMIILSHNHFDHTGGLFGIMKEINKEIPIFAHPNIFKVSFATEPEFMLAGTLNKTLKEDIEKLGGRWVLSRDPIRLMPGIFTLGEIEDEEKINFEKKPTIGLYKLENGRVVLDNVEDEIGLAIVTEKGLIIVSGCSHPGIVSMVKKSIKISGINKVYAVIGGFHLIDADNERIVSTIKALKKLGVKKICTGHCTGFKAENMFMEEFKEDFERLHAGKIIKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q57768</accession>
    <name>ENGB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">engB</name>
        <name type="ordered locus">MJ0320</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64340">
        <property type="entry name" value="A64340"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57768"/>
    <dbReference type="SMR" id="Q57768"/>
    <dbReference type="STRING" id="243232.MJ_0320"/>
    <dbReference type="EnsemblBacteria" id="AAB98305">
        <property type="protein sequence ID" value="AAB98305"/>
        <property type="gene ID" value="MJ_0320"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0320"/>
    <dbReference type="eggNOG" id="arCOG00355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033732_3_0_2"/>
    <dbReference type="InParanoid" id="Q57768"/>
    <dbReference type="OMA" id="PNHYDWA"/>
    <dbReference type="PhylomeDB" id="Q57768"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000157810" description="Probable GTP-binding protein EngB">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="EngB-type G" evidence="1">
        <location>
            <begin position="23"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="57"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="74"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="154"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="189"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <sequence length="219" mass="25615" checksum="0603A761767B117E" modified="1996-11-01" version="1">MCETMDFFERYKNLKEKYEEKKTKPKVIVVGRSNVGKSTFVRLMTGRKDIRVGKKPGVTLKINEYDMGEYILVDMPGFGYMAGLPKKVQEKIKDEIVHYIEEHADEIAAAVQIIDTKSFFEIVERWKGRGEIPIDLEMFDFITDLKISPILVANKMDKIKKEEWDAVLDGICEYLKCQPPWHQWKFIVPAILKEGKGIEEIKKKILERVRLFKKLRGIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q57780</accession>
    <name>Y334_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0334</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0334</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64341">
        <property type="entry name" value="F64341"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57780"/>
    <dbReference type="SMR" id="Q57780"/>
    <dbReference type="STRING" id="243232.MJ_0334"/>
    <dbReference type="EnsemblBacteria" id="AAB98322">
        <property type="protein sequence ID" value="AAB98322"/>
        <property type="gene ID" value="MJ_0334"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0334"/>
    <dbReference type="eggNOG" id="arCOG08301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176742_0_0_2"/>
    <dbReference type="OMA" id="HEMEQFY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106804" description="Uncharacterized protein MJ0334">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <sequence length="102" mass="11555" checksum="1C9A45826A0B2AE3" modified="1996-11-01" version="1">MSKMGLFKKEKDNGVIDDLVPKKSDYVEKIAQEAIRKDKVDALVRKHEMEQFYHEVSQNEKIAELKAELAKKEKEIEELKEEIRGLKGKAGGLGIGGSQVTR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57795</accession>
    <name>Y349_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0349</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0349</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the M.jannaschii MJ0023/MJ0349/MJ1072/MJ1074/MJ1107/MJECL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64343">
        <property type="entry name" value="E64343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869848.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57795"/>
    <dbReference type="SMR" id="Q57795"/>
    <dbReference type="STRING" id="243232.MJ_0349"/>
    <dbReference type="EnsemblBacteria" id="AAB98338">
        <property type="protein sequence ID" value="AAB98338"/>
        <property type="gene ID" value="MJ_0349"/>
    </dbReference>
    <dbReference type="GeneID" id="1451206"/>
    <dbReference type="KEGG" id="mja:MJ_0349"/>
    <dbReference type="eggNOG" id="arCOG09652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165881_0_0_2"/>
    <dbReference type="OMA" id="YKLIIHT"/>
    <dbReference type="OrthoDB" id="123012at2157"/>
    <dbReference type="PhylomeDB" id="Q57795"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106820" description="Uncharacterized protein MJ0349">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="105" mass="12195" checksum="8A75B451662B963C" modified="1997-11-01" version="1">MAVAYAKLYELILKKVKDEKEAEELYNAIIEIVKEEKLAVKTELKDELRGELATKEDIKYLDGKIEMVKKELEYKLIIHTLIILFAIIITNPNAIELIKLLFGFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57796</accession>
    <name>Y350_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0350</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0350</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64343">
        <property type="entry name" value="F64343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869849.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57796"/>
    <dbReference type="SMR" id="Q57796"/>
    <dbReference type="STRING" id="243232.MJ_0350"/>
    <dbReference type="EnsemblBacteria" id="AAB98339">
        <property type="protein sequence ID" value="AAB98339"/>
        <property type="gene ID" value="MJ_0350"/>
    </dbReference>
    <dbReference type="GeneID" id="1451207"/>
    <dbReference type="KEGG" id="mja:MJ_0350"/>
    <dbReference type="eggNOG" id="arCOG09653">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2581398_0_0_2"/>
    <dbReference type="OMA" id="CRTERIE"/>
    <dbReference type="OrthoDB" id="139967at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106821" description="Uncharacterized protein MJ0350">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <sequence length="88" mass="10593" checksum="58E111FC57ECA619" modified="1996-11-01" version="1">MAKVRHGCRTERIELIIKINHLKRENLKLRNKIADYKRTLYLIRGKGCKLTEELLKIEIENCIKMIKFNEETIRDLKEKVEMIPNEQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q57802</accession>
    <name>Y356_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0356</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0356</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64344">
        <property type="entry name" value="D64344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869855.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57802"/>
    <dbReference type="STRING" id="243232.MJ_0356"/>
    <dbReference type="EnsemblBacteria" id="AAB98348">
        <property type="protein sequence ID" value="AAB98348"/>
        <property type="gene ID" value="MJ_0356"/>
    </dbReference>
    <dbReference type="GeneID" id="1451213"/>
    <dbReference type="KEGG" id="mja:MJ_0356"/>
    <dbReference type="eggNOG" id="arCOG09654">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2519748_0_0_2"/>
    <dbReference type="OMA" id="WITKMIT"/>
    <dbReference type="OrthoDB" id="138356at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106827" description="Uncharacterized protein MJ0356">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <sequence length="97" mass="10738" checksum="2020A0628CBE1BE0" modified="1996-11-01" version="1">MALKEFGGLIMKFLVVKKGSETKRINVEEIKEISNVGSFVIIRYSGGEVKIKAKGDAEEAADWITKMITDFPNKIIDLRAGFESGDKAKTNLRVEAC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q57825</accession>
    <name>Y380_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0380</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0380</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64347">
        <property type="entry name" value="D64347"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57825"/>
    <dbReference type="SMR" id="Q57825"/>
    <dbReference type="STRING" id="243232.MJ_0380"/>
    <dbReference type="EnsemblBacteria" id="AAB98377">
        <property type="protein sequence ID" value="AAB98377"/>
        <property type="gene ID" value="MJ_0380"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0380"/>
    <dbReference type="eggNOG" id="arCOG06572">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1891544_0_0_2"/>
    <dbReference type="OMA" id="IRNIGRY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106843" description="Uncharacterized protein MJ0380">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <sequence length="118" mass="13758" checksum="322C1759E495B1DD" modified="1996-11-01" version="1">MSIDEVIKLENWMKNIGRYLSYLISDKFEEYAYDIIDGVAKARNANELLEALYKGLRLSPKLKKKGNIEVPSPEDVKKLEETLREIGDNEKEVRKIGLSMALWAFADWEKDHRKRGDQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q57852</accession>
    <name>Y650_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0650</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0650</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64381">
        <property type="entry name" value="B64381"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57852"/>
    <dbReference type="STRING" id="243232.MJ_0650"/>
    <dbReference type="EnsemblBacteria" id="AAB98647">
        <property type="protein sequence ID" value="AAB98647"/>
        <property type="gene ID" value="MJ_0650"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0650"/>
    <dbReference type="HOGENOM" id="CLU_397742_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013992" description="Uncharacterized protein MJ0650">
        <location>
            <begin position="25"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="692" mass="80607" checksum="25D0A91A08177188" modified="1997-11-01" version="2" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57878</accession>
    <name>ATGT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="1">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tgtA</name>
        <name type="ordered locus">MJ0436</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2000" name="J. Biol. Chem." volume="275" first="28731" last="28738">
            <title>Hypermodification of tRNA in Thermophilic archaea. Cloning, overexpression, and characterization of tRNA-guanine transglycosylase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bai Y."/>
                <person name="Fox D.T."/>
                <person name="Lacy J.A."/>
                <person name="Van Lanen S.G."/>
                <person name="Iwata-Reuyl D."/>
            </authorList>
            <dbReference type="PubMed" id="10862614"/>
            <dbReference type="DOI" id="10.1074/jbc.M002174200"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATES</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs. Can also utilize guanine as substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>7-cyano-7-deazaguanine + guanosine(15) in tRNA = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 5.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 85 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAB98424" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98424.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64354">
        <property type="entry name" value="D64354"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57878"/>
    <dbReference type="SMR" id="Q57878"/>
    <dbReference type="STRING" id="243232.MJ_0436"/>
    <dbReference type="EnsemblBacteria" id="AAB98424">
        <property type="protein sequence ID" value="AAB98424"/>
        <property type="gene ID" value="MJ_0436"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0436"/>
    <dbReference type="eggNOG" id="arCOG00989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00991">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030083_0_0_2"/>
    <dbReference type="InParanoid" id="Q57878"/>
    <dbReference type="OMA" id="FPCSCPV"/>
    <dbReference type="PhylomeDB" id="Q57878"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-16210"/>
    <dbReference type="BRENDA" id="2.4.2.48">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008616">
        <property type="term" value="P:queuosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1020.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038370">
        <property type="entry name" value="ArcTGT_C1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="SSF51713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000135572" description="tRNA-guanine(15) transglycosylase">
        <location>
            <begin position="1"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="domain" description="PUA">
        <location>
            <begin position="577"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10862614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="655" mass="76076" checksum="7BE489600334AF62" modified="2006-07-25" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57881</accession>
    <name>Y439_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0439</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0439</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64354">
        <property type="entry name" value="G64354"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57881"/>
    <dbReference type="SMR" id="Q57881"/>
    <dbReference type="DNASU" id="1451299"/>
    <dbReference type="EnsemblBacteria" id="AAB98434">
        <property type="protein sequence ID" value="AAB98434"/>
        <property type="gene ID" value="MJ_0439"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0439"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q57881"/>
    <dbReference type="OMA" id="WCERELT"/>
    <dbReference type="PhylomeDB" id="Q57881"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184668" description="Uncharacterized ATP-binding protein MJ0439">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="361" mass="42610" checksum="8B24E3B24F1E5D80" modified="1997-11-01" version="1">MNYIYSFINGDFMRFFNREKEITEILSILEGNPDLVYFVYGPLNSGKTALISEIINNRIDKNKYVVFYINLRGIFISKYKDFIEVLFEEYEEDRKPVEIIKSLIKDVPSLCGIPTPKNTLEEILKKKTTKNVFKYITNVLMDIKKEGKQPIIIIDELQKIGDMKINGFLIYELFNYFVDLTKELHLCHVFCLSSDSLFIEQVYSEAMLKDRVDYILVDDFDKETALKFMDFLAEEILNKKLSDDEKELIYSYVGGKPILIIKVIKKLKIKGLKETLDEMLRDEMQKLKYFLEDIKEKDEESYNKIADALEIFKDSYEIEDIKIPKNIREFLVKKNILFLNPQKGTLKPQSYLVWNAIKRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57891</accession>
    <name>Y449_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter MJ0449</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0449</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64356">
        <property type="entry name" value="A64356"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869948.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57891"/>
    <dbReference type="SMR" id="Q57891"/>
    <dbReference type="STRING" id="243232.MJ_0449"/>
    <dbReference type="EnsemblBacteria" id="AAB98438">
        <property type="protein sequence ID" value="AAB98438"/>
        <property type="gene ID" value="MJ_0449"/>
    </dbReference>
    <dbReference type="GeneID" id="1451309"/>
    <dbReference type="KEGG" id="mja:MJ_0449"/>
    <dbReference type="eggNOG" id="arCOG01474">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_3_3_2"/>
    <dbReference type="InParanoid" id="Q57891"/>
    <dbReference type="OMA" id="IEMHLIV"/>
    <dbReference type="OrthoDB" id="110591at2157"/>
    <dbReference type="PhylomeDB" id="Q57891"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="SSF160240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="SSF161111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000206139" description="Uncharacterized transporter MJ0449">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="283" mass="31286" checksum="4F9128C4222365FE" modified="1996-11-01" version="1">MREVEKPLILSIVGNILLGLIKIIIGYVYSSISLISDGIHSLSDVITSIIGIIGVKIASKPPDESHPYGHSRFECLFSFFIGLALFFTAYEIGKFAVERIIYGEVIEVNAIMVGVAILSIVVKELMTRYSLFVGKKLNSQVLIADAYHHRSDALSSVVVLVGLLLQKFGIYYGDAIAGIIVALMIAKVAFDICLTNIDYLTGRAPPKKFFELIEKEALNVDKVIGVHDIKAHYVGPRIHVELHVEVPSNISAKEMHDIEVAVKNRLESLENVERAYVHVDIVD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57926</accession>
    <name>AKSA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Homocitrate synthase AksA</fullName>
            <ecNumber evidence="2">2.3.3.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>(R)-homo(2)citrate synthase</fullName>
            <ecNumber evidence="2">2.3.3.-</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName>(R)-homo(3)citrate synthase</fullName>
            <ecNumber evidence="2">2.3.3.-</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">aksA</name>
        <name type="ordered locus">MJ0503</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Biochemistry" volume="37" first="10108" last="10117">
            <title>Alpha-keto acid chain elongation reactions involved in the biosynthesis of coenzyme B (7-mercaptoheptanoyl threonine phosphate) in methanogenic Archaea.</title>
            <authorList>
                <person name="Howell D.M."/>
                <person name="Harich K."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="9665716"/>
            <dbReference type="DOI" id="10.1021/bi980662p"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-5</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of alpha-ketoglutarate and acetyl-CoA to form (R)-homocitrate. Can also catalyze the condensation of alpha-ketoadipate with acetyl-CoA to form (R)-homo(2)citrate, and the condensation of alpha-ketopimelate with acetyl-CoA to form (R)-homo(3)citrate. These reactions are part of the biosynthesis pathway of coenzyme B and biotin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoglutarate + acetyl-CoA + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoadipate + acetyl-CoA + H2O = (R)-dihomocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44924"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57499"/>
            <dbReference type="ChEBI" id="CHEBI:72697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:44925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-oxoheptanedioate + acetyl-CoA + H2O = (R)-trihomocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44928"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:72699"/>
            <dbReference type="ChEBI" id="CHEBI:72701"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:44929"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Organic acid metabolism; 2-oxosuberate biosynthesis.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">Has been shown to catalyze the formation of trans-homoaconitate (PubMed:9665716). However, probably physiologically functions as an (R)-homocitrate/(R)-dihomocitrate/(R)-trihomocitrate synthase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.14" evidence="2"/>
    <dbReference type="EC" id="2.3.3.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64362">
        <property type="entry name" value="G64362"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57926"/>
    <dbReference type="SMR" id="Q57926"/>
    <dbReference type="STRING" id="243232.MJ_0503"/>
    <dbReference type="EnsemblBacteria" id="AAB98494">
        <property type="protein sequence ID" value="AAB98494"/>
        <property type="gene ID" value="MJ_0503"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0503"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_4_2_2"/>
    <dbReference type="InParanoid" id="Q57926"/>
    <dbReference type="PhylomeDB" id="Q57926"/>
    <dbReference type="UniPathway" id="UPA00919"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000140410" description="Homocitrate synthase AksA">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="1">
        <location>
            <begin position="32"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="9665716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="9665716"/>
        </source>
    </evidence>
    <sequence length="406" mass="45365" checksum="F50D71024B188157" modified="2011-04-05" version="3">MTKVLVMFMDFLFENSWKAVCPYNPKLDLKDIYIYDTTLRDGEQTPGVCFTKEQKLEIARKLDELGLKQIEAGFPIVSEREADIVKTIANEGLNADILALCRALKKDIDKAIECDVDGIITFIATSPLHLKYKFNNKSLDEILEMGVEAVEYAKEHGLFVAFSAEDATRTPIEDLIKVHKAAEEAGADRVHIADTTGCATPQSMEFICKTLKENLKKAHIGVHCHNDFGFAVINSIYGLIGGAKAVSTTVNGIGERAGNAALEELIMALTVLYDVDLGLNLEVLPELCRMVEEYSGIKMPKNKPIVGELVFAHESGIHVDAVIENPLTYEPFLPEKIGLKRNILLGKHSGCRAVAYKLKLMGIDYDREMLCEIVKKVKEIREEGKFITDEVFKEIVEEVLRKRNKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57934</accession>
    <name>Y514_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized polyferredoxin-like protein MJ0514</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0514</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64364">
        <property type="entry name" value="B64364"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870015.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57934"/>
    <dbReference type="STRING" id="243232.MJ_0514"/>
    <dbReference type="EnsemblBacteria" id="AAB98503">
        <property type="protein sequence ID" value="AAB98503"/>
        <property type="gene ID" value="MJ_0514"/>
    </dbReference>
    <dbReference type="GeneID" id="1451376"/>
    <dbReference type="KEGG" id="mja:MJ_0514"/>
    <dbReference type="eggNOG" id="arCOG02179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097041_0_0_2"/>
    <dbReference type="InParanoid" id="Q57934"/>
    <dbReference type="OMA" id="CVKCEIC"/>
    <dbReference type="OrthoDB" id="93710at2157"/>
    <dbReference type="PhylomeDB" id="Q57934"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159148" description="Uncharacterized polyferredoxin-like protein MJ0514">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="69"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 3" evidence="2">
        <location>
            <begin position="124"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 4" evidence="2">
        <location>
            <begin position="154"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 5" evidence="2">
        <location>
            <begin position="191"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 6" evidence="2">
        <location>
            <begin position="220"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
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            <position position="133"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
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            <position position="139"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
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        </ligand>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
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            <position position="200"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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    </feature>
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        <location>
            <position position="206"/>
        </location>
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            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="250" mass="28246" checksum="5C4D611D53ED2960" modified="1996-11-01" version="1">MITLIEIKKSLDEILSKIDGDKKYINEVAKKITPITYKLLYINETKCIRCNLCYKECPVDAIEKAKVKKSAKIIEDKCVKCEICAQTCPVGAIYVIEGRAEIEDSEVHYTIKEKSIPHRKIRLKKYELDENTCIKCGICARFCPTNAIKAVRRKSIEVNLDLCMGCGACAEVCPKKCIKVERELGEVIKTRDIEVDKNLCVGCLVCIEECPINAIDQDGDKVKINKDKCILCGRCVDVCPTNAIKMWEKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57967</accession>
    <name>Y547_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0547</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0547</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64368">
        <property type="entry name" value="C64368"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870051.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57967"/>
    <dbReference type="SMR" id="Q57967"/>
    <dbReference type="STRING" id="243232.MJ_0547"/>
    <dbReference type="EnsemblBacteria" id="AAB98539">
        <property type="protein sequence ID" value="AAB98539"/>
        <property type="gene ID" value="MJ_0547"/>
    </dbReference>
    <dbReference type="GeneID" id="1451412"/>
    <dbReference type="KEGG" id="mja:MJ_0547"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="InParanoid" id="Q57967"/>
    <dbReference type="OMA" id="CESAKAY"/>
    <dbReference type="OrthoDB" id="105856at2157"/>
    <dbReference type="PhylomeDB" id="Q57967"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106925" description="Uncharacterized ATP-binding protein MJ0547">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="15"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q72H90"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="264" mass="27737" checksum="B9AF5108CDD0CEC3" modified="1996-11-01" version="1">MVTLMAIAIAIASGKGGTGKTTISANLAVALAKFGKKVAVLDADIAMANLELIMGLEGKPVTLNDVLAGKADIKDAIYEGPEGVLVIPAGVSLEKFRRAKPEKLEEVLKAIHDLVEILIIDCPAGIGKETLIAISSADGLIVVVNPEISSISDALKIIAITKRLGTDIIGAIVNRVSNESTELGVKAIETILEVPVIGVVPEDPHVRKAAAFGTPLVIMYPDSPAAQAIMEIAAKLIGAKYEAQLKKKKESFISKFIKGLFGRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q57986</accession>
    <name>FEOB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Fe(2+) transporter FeoB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Ferrous iron transport protein B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0566</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference evidence="6 7 8" key="2">
        <citation type="journal article" date="2009" name="J. Mol. Biol." volume="392" first="405" last="419">
            <title>Structure and function of the FeoB G-domain from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Koster S."/>
                <person name="Wehner M."/>
                <person name="Herrmann C."/>
                <person name="Kuhlbrandt W."/>
                <person name="Yildiz O."/>
            </authorList>
            <dbReference type="PubMed" id="19615379"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2009.07.020"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 1-165 IN COMPLEX WITH AND WITHOUT GUANINE NUCLEOTIDES</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <scope>GDP-BINDING</scope>
        <scope>MUTAGENESIS OF LYS-41 AND TYR-61</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Probable transporter of a GTP-driven Fe(2+) uptake system, might be able to transport Fe(2+) into or out of the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">The crystallized N-terminal domain is a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="1 4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">The G protein domain (about resides 1-184) binds GDP faster and with higher affinity than GTP and releases GTP much faster than GDP. Release of bound nucleotide causes a rearrangement of GTP-binding domain G5, which may transmit information about the nucleotide-bound state from the G-domain to the transmembrane region of FeoB and effect Fe(2+) transport.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. FeoB GTPase (TC 9.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64370">
        <property type="entry name" value="F64370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870070.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2WJG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-184"/>
    </dbReference>
    <dbReference type="PDB" id="2WJH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="2WJI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B=1-165"/>
    </dbReference>
    <dbReference type="PDB" id="2WJJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-167"/>
    </dbReference>
    <dbReference type="PDBsum" id="2WJG"/>
    <dbReference type="PDBsum" id="2WJH"/>
    <dbReference type="PDBsum" id="2WJI"/>
    <dbReference type="PDBsum" id="2WJJ"/>
    <dbReference type="AlphaFoldDB" id="Q57986"/>
    <dbReference type="SMR" id="Q57986"/>
    <dbReference type="STRING" id="243232.MJ_0566"/>
    <dbReference type="TCDB" id="9.A.8.1.9">
        <property type="family name" value="the ferrous iron uptake (feob) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98557">
        <property type="protein sequence ID" value="AAB98557"/>
        <property type="gene ID" value="MJ_0566"/>
    </dbReference>
    <dbReference type="GeneID" id="1451431"/>
    <dbReference type="KEGG" id="mja:MJ_0566"/>
    <dbReference type="eggNOG" id="arCOG00359">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013350_3_0_2"/>
    <dbReference type="InParanoid" id="Q57986"/>
    <dbReference type="OMA" id="MEMPDYE"/>
    <dbReference type="OrthoDB" id="27688at2157"/>
    <dbReference type="PhylomeDB" id="Q57986"/>
    <dbReference type="EvolutionaryTrace" id="Q57986"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
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    <sequence length="668" mass="74455" checksum="2145495E1150C19E" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
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            <fullName>Uncharacterized protein MJ0578</fullName>
        </recommendedName>
    </protein>
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        <name type="ordered locus">MJ0578</name>
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    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
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            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
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            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="276" mass="30794" checksum="0F900CE7F2E88669" modified="1996-11-01" version="1">MDYLGIIMKIAIISGKGGVGKSSISTSLAKLFSKEFNIVALDCDVDAPNFNLMFDVKDKKLLEVIYREIYEINDDCIRCGKCLDVCQFDAIGDFKINPILCEGCGACELICEFDAIEPIKRESGYIYEGFVGFPLIWGELEVGESGSGKIIEHIKNHAKKYKAELGIIDGPPGVGCPLISTVKDVDLALCIVEPTKSSVNDCLRLIETLNFFNVEYLIVENKKGMNNINYPFKIFHSIPFDFDVPKLIANKILLCDSNSKVSESIKELYEKLKEFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58000</accession>
    <name>Y580_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0580</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0580</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64372">
        <property type="entry name" value="D64372"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870084.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58000"/>
    <dbReference type="SMR" id="Q58000"/>
    <dbReference type="STRING" id="243232.MJ_0580"/>
    <dbReference type="EnsemblBacteria" id="AAB98571">
        <property type="protein sequence ID" value="AAB98571"/>
        <property type="gene ID" value="MJ_0580"/>
    </dbReference>
    <dbReference type="GeneID" id="1451445"/>
    <dbReference type="KEGG" id="mja:MJ_0580"/>
    <dbReference type="eggNOG" id="arCOG02734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104194_2_3_2"/>
    <dbReference type="InParanoid" id="Q58000"/>
    <dbReference type="OMA" id="QFCIDQG"/>
    <dbReference type="OrthoDB" id="118295at2157"/>
    <dbReference type="PhylomeDB" id="Q58000"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00851">
        <property type="entry name" value="MTH1175"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033913">
        <property type="entry name" value="MTH1175_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106941" description="Uncharacterized protein MJ0580">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <sequence length="118" mass="13139" checksum="71B7E11FF73E0C1F" modified="1996-11-01" version="1">MTKILKVKLMKIALPIDNNRLSPHFGRCEKFMIVEIENGEIKNKEIIENTARNGMHGVGTTSASLIANMGVNAIIVQNIGPKAYSVFKQLGIDVYKANTTSIDECIKLFLEGKLEKFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58018</accession>
    <name>THI4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="2 3">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">thi4</name>
        <name type="ordered locus">MJ0601</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Bacteriol." volume="186" first="6360" last="6366">
            <title>Modified pathway to synthesize ribulose 1,5-bisphosphate in methanogenic archaea.</title>
            <authorList>
                <person name="Finn M.W."/>
                <person name="Tabita F.R."/>
            </authorList>
            <dbReference type="PubMed" id="15375115"/>
            <dbReference type="DOI" id="10.1128/JB.186.19.6360-6366.2004"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF N-TERMINUS</scope>
        <scope>PRELIMINARY FUNCTION IN CO(2) FIXATION WITH RUBISCO</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2016" name="J. Am. Chem. Soc." volume="138" first="3639" last="3642">
            <title>From suicide enzyme to catalyst: The iron-dependent sulfide transfer in Methanococcus jannaschii thiamin thiazole biosynthesis.</title>
            <authorList>
                <person name="Eser B.E."/>
                <person name="Zhang X."/>
                <person name="Chanani P.K."/>
                <person name="Begley T.P."/>
                <person name="Ealick S.E."/>
            </authorList>
            <dbReference type="PubMed" id="26928142"/>
            <dbReference type="DOI" id="10.1021/jacs.6b00445"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>PATHWAY</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2016" name="Biochemistry" volume="55" first="1826" last="1838">
            <title>Structural basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases.</title>
            <authorList>
                <person name="Zhang X."/>
                <person name="Eser B.E."/>
                <person name="Chanani P.K."/>
                <person name="Begley T.P."/>
                <person name="Ealick S.E."/>
            </authorList>
            <dbReference type="PubMed" id="26919468"/>
            <dbReference type="DOI" id="10.1021/acs.biochem.6b00030"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.71 ANGSTROMS) IN COMPLEX WITH ADP-RIBULOSE</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>MUTAGENESIS OF HIS-164</scope>
        <scope>LACK OF RUBP SYNTHASE ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>glycine + hydrogen sulfide + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + H(+) + 3 H2O + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="3">Binds 1 Fe(2+) ion per subunit. Other divalent metal cations can support activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">Unlike THI4 from S.cerevisiae, Thi4 from M.jannaschii can catalyze multiple turnovers because the sulfide is not enzyme derived.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6 7">This protein was originally thought to have NAD-dependent ribose 1,5-bisphosphate isomerase activity but the recombinant protein was not active in vitro (PubMed:15375115, PubMed:26919468). In contrast, another protein from M.jannaschii likely possesses this activity (MJ0122).</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="2 3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64375">
        <property type="entry name" value="A64375"/>
    </dbReference>
    <dbReference type="PDB" id="4Y4M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.71 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-267"/>
    </dbReference>
    <dbReference type="PDBsum" id="4Y4M"/>
    <dbReference type="AlphaFoldDB" id="Q58018"/>
    <dbReference type="SMR" id="Q58018"/>
    <dbReference type="STRING" id="243232.MJ_0601"/>
    <dbReference type="EnsemblBacteria" id="AAB98592">
        <property type="protein sequence ID" value="AAB98592"/>
        <property type="gene ID" value="MJ_0601"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0601"/>
    <dbReference type="eggNOG" id="arCOG00574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_2_0_2"/>
    <dbReference type="InParanoid" id="Q58018"/>
    <dbReference type="PhylomeDB" id="Q58018"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-21800"/>
    <dbReference type="BRENDA" id="2.4.2.59">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.4.2.60">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00292">
        <property type="entry name" value="TIGR00292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153947" description="Thiamine thiazole synthase">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="66"/>
            <end position="67"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Still requires free sulfide for ADT synthesis, showing that this cysteine cannot act as an enzyme-derived sulfur source for thiazole formation as in S.cerevisiae." evidence="2">
        <original>H</original>
        <variation>C</variation>
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="36"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="62"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="74"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="82"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="turn" evidence="9">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="109"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="147"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="156"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="184"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="219"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="225"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="230"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="246"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="26919468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="26928142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="26928142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="15375115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="26919468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="26928142"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="4Y4M"/>
        </source>
    </evidence>
    <sequence length="267" mass="28655" checksum="2F359B900C838251" modified="2010-11-02" version="3">MVNLMNIKDIKLNADETKTTKAILKASFDMWLDIVEADVVIVGAGPSGLTCARYLAKEGFKVVVLERHLAFGGGTWGGGMGFPYIVVEEPADELLREVGIKLIDMGDGYYVADSVEVPAKLAVAAMDAGAKILTGIVVEDLILREDGVAGVVINSYAIERAGLHIDPLTIRSKVVVDATGHEASIVNILVKKNKLEADVPGEKSMWAEKGENALLRNTREVYPNLFVCGMAANASHGGYRMGAIFGGMYLSGKLCAELITEKLKNKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58036</accession>
    <name>HMPTM_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin dimethyltransferase</fullName>
            <shortName evidence="5">6-hydroxymethyl-H(2)pterin dimethyltransferase</shortName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0619</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="J. Bacteriol." volume="196" first="3315" last="3323">
            <title>Identification of a unique radical S-Adenosylmethionine methylase likely involved in methanopterin biosynthesis in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Allen K.D."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="25002541"/>
            <dbReference type="DOI" id="10.1128/JB.01903-14"/>
        </citation>
        <scope>FUNCTION AS A METHYLTRANSFERASE</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>MUTAGENESIS OF CYS-77 AND CYS-102</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Is responsible for the addition of methyl groups at C-7 and C-9 of the pterin ring during methanopterin (MPT) biosynthesis. Catalyzes methylation of 7,8-dihydro-6-hydroxymethylpterin, likely using methylenetetrahydromethanopterin as a methyl group donor, via a radical-based mechanism.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 2 [4Fe-4S] clusters. The clusters are coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="5">Binds 2 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">The most N-terminal iron-sulfur cluster is involved in C-9 methylation, while the second cluster is required for C-7 methylation of the pterin ring. Moreover, C-7 methylation occurs before C-9 methylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the radical SAM superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64377">
        <property type="entry name" value="C64377"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870124.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58036"/>
    <dbReference type="SMR" id="Q58036"/>
    <dbReference type="STRING" id="243232.MJ_0619"/>
    <dbReference type="DNASU" id="1451485"/>
    <dbReference type="EnsemblBacteria" id="AAB98614">
        <property type="protein sequence ID" value="AAB98614"/>
        <property type="gene ID" value="MJ_0619"/>
    </dbReference>
    <dbReference type="GeneID" id="1451485"/>
    <dbReference type="KEGG" id="mja:MJ_0619"/>
    <dbReference type="eggNOG" id="arCOG00933">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023791_0_0_2"/>
    <dbReference type="InParanoid" id="Q58036"/>
    <dbReference type="OMA" id="RCDIHYA"/>
    <dbReference type="OrthoDB" id="13164at2157"/>
    <dbReference type="PhylomeDB" id="Q58036"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-18796"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034471">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034474">
        <property type="entry name" value="Methyltransferase_Class_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306">
        <property type="entry name" value="PTHR43306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00385">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01100">
        <property type="entry name" value="methyltransferase_(Class_D)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106960" description="7,8-dihydro-6-hydroxymethylpterin dimethyltransferase">
        <location>
            <begin position="1"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="82"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Loss of C9-methylation but C7-methylation is still observed." evidence="2">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="77"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of methylation activity." evidence="2">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="25002541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="25002541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="25002541"/>
        </source>
    </evidence>
    <sequence length="506" mass="57482" checksum="25FA1F6A380398D8" modified="1996-11-01" version="1">MEKKTLSLCPICLKRIPATILEEDGKIIIKKTCPEHGEFKDIYWGDAELYKKFDKYEFIGKIEVTNTKVKNGCPYDCGLCPNHKSTTILANIDVTNRCNLNCPICFANANKSGKVYEPSFEDIKRMMENLRKEIPPTPAIQFAGGEPTVRSDLPELIKLARDMGFLHVQLATNGIKLKNINYLKKLKEAGLSTIYLQFDGISEKPYLVARGKNLLPIKQKVIENCKKVGFDSVVLVPTLVRGVNDNEVGGIIRYAAENVDVVRGINFQPVSFTGRVDEKTLLEGRITIPDFIKLVEEQTDGEITEEDFYPVPSVAPISVLVEKLTNDRKPTLSSHQHCGTSTYVFVDEDGKLIPITRFIDVEGFLEIVKEKIEEIGKSKMHDVKVLGEIALKLPSLIDLDKAPKSVNIKKIIDLILSVLKSDYSALAELHYHMLMISCMHFMDAYNFDVKRVMRCCIHYATPDDRIIPFCTYNTLHRQEVEEKFSIPLEEWKRMHKIGGEDDREDY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58078</accession>
    <name>Y664_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0664</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0664</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64382">
        <property type="entry name" value="H64382"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58078"/>
    <dbReference type="SMR" id="Q58078"/>
    <dbReference type="STRING" id="243232.MJ_0664"/>
    <dbReference type="EnsemblBacteria" id="AAB98660">
        <property type="protein sequence ID" value="AAB98660"/>
        <property type="gene ID" value="MJ_0664"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0664"/>
    <dbReference type="eggNOG" id="arCOG05045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_920144_0_0_2"/>
    <dbReference type="OMA" id="ANRDYNW"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003916">
        <property type="term" value="F:DNA topoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="zf-C4_Topoisom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106980" description="Uncharacterized protein MJ0664">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <sequence length="301" mass="35495" checksum="CF1D742AB59F1CD2" modified="1996-11-01" version="1">MKNLVLRRDNMGEVLNELFEILSKDLHFNATKLNSETYEQNWKVPEGFISIIGIENAKMPVFYYGITNSYKKEFEIKKVISPKGQKQIFARIYYIFDRRDIIEKEKYVVANAFNGLLLLIKFDKTEFIEKAIEMLTKGYEEDNFIKEVLNNIKEKNMFDNIDETIRFVANRDYNWLIGRIKYNMGILEYSGQGYYLIPIKTNMQITPGIKINNGRVIIDLENSHLFKNFIVYVDTINNKIIYNKERLCNKNPAILDIMSKIDDNTCPWCGAKLRVVRTKKGEFLGCTNYPNCLYRRFPKKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58096</accession>
    <name>Y683_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0683</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0683</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64385">
        <property type="entry name" value="C64385"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58096"/>
    <dbReference type="SMR" id="Q58096"/>
    <dbReference type="STRING" id="243232.MJ_0683"/>
    <dbReference type="EnsemblBacteria" id="AAB98678">
        <property type="protein sequence ID" value="AAB98678"/>
        <property type="gene ID" value="MJ_0683"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0683"/>
    <dbReference type="eggNOG" id="arCOG01290">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015525_2_2_2"/>
    <dbReference type="InParanoid" id="Q58096"/>
    <dbReference type="OMA" id="CYARLYT"/>
    <dbReference type="PhylomeDB" id="Q58096"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040086">
        <property type="entry name" value="MJ0683-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43432">
        <property type="entry name" value="PTHR43432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01084">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000106987" description="Uncharacterized protein MJ0683">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="19"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="259" mass="30385" checksum="15CB7E77D2CF756C" modified="1996-11-01" version="1">MNSKIEIIKIKAKKPINPTKIPGAKYVINQYIGCQYACKYCYARFMCKWYNYGKWGSWVVVKENLPDLIKNKHIKGKIYMSSVSDAYRPIEKDFKLTRNILKNIDKRAELSILTKSDLVLRDMDLFKKFSSIEVGLTINNFEGNLKKDIEPFSPSNEKRIDALKTLYENGIKNYAFISPIIPDLIDVEYIIGETKPFTNFYYFEFLNLKASREFKHYLEQNYPESYEIISNKTAFKRYIDEVINTIKKKDIAIKGICVH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58127</accession>
    <name>Y717_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0717</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0717</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To Synechocystis PCC 6803 slr0903.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64389">
        <property type="entry name" value="E64389"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870222.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58127"/>
    <dbReference type="SMR" id="Q58127"/>
    <dbReference type="STRING" id="243232.MJ_0717"/>
    <dbReference type="EnsemblBacteria" id="AAB98711">
        <property type="protein sequence ID" value="AAB98711"/>
        <property type="gene ID" value="MJ_0717"/>
    </dbReference>
    <dbReference type="GeneID" id="1451594"/>
    <dbReference type="KEGG" id="mja:MJ_0717"/>
    <dbReference type="eggNOG" id="arCOG00534">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089568_1_2_2"/>
    <dbReference type="InParanoid" id="Q58127"/>
    <dbReference type="OMA" id="HRKEGFE"/>
    <dbReference type="OrthoDB" id="62565at2157"/>
    <dbReference type="PhylomeDB" id="Q58127"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="SSF54690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107002" description="Uncharacterized protein MJ0717">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="119" mass="14062" checksum="D9943113FBFC5B45" modified="1996-11-01" version="1">MIFNEYEEFCKKMDECIEKYKGKFGCIVTFNGFVREYDLKDGEKVPSKGMKIDEDILEKLKLVIEEAKNKFDVIDILFYHNTGFLSIGERIASIAVFARHRKEGFEALEYIINEMKKYH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q58131</accession>
    <name>ARGD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetylornithine aminotransferase</fullName>
            <shortName evidence="1">ACOAT</shortName>
            <ecNumber evidence="1">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argD</name>
        <name type="ordered locus">MJ0721</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-oxoglutarate + N(2)-acetyl-L-ornithine = L-glutamate + N-acetyl-L-glutamate 5-semialdehyde</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="1">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64390">
        <property type="entry name" value="A64390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870226.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58131"/>
    <dbReference type="SMR" id="Q58131"/>
    <dbReference type="STRING" id="243232.MJ_0721"/>
    <dbReference type="EnsemblBacteria" id="AAB98717">
        <property type="protein sequence ID" value="AAB98717"/>
        <property type="gene ID" value="MJ_0721"/>
    </dbReference>
    <dbReference type="GeneID" id="1451598"/>
    <dbReference type="KEGG" id="mja:MJ_0721"/>
    <dbReference type="eggNOG" id="arCOG00914">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_2"/>
    <dbReference type="InParanoid" id="Q58131"/>
    <dbReference type="OMA" id="MVPNYNP"/>
    <dbReference type="OrthoDB" id="20774at2157"/>
    <dbReference type="PhylomeDB" id="Q58131"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000112821" description="Acetylornithine aminotransferase">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="223"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <sequence length="398" mass="44065" checksum="D9EF588A9B5C3EEF" modified="1996-11-01" version="1">MSQENWIDLEKKYHLQIYGRLPVVLVEGKGMEVYDIDGKKYLDFLAGIGVNNVGHCHPKVVEAIKKQAETLIHTSNIYYTIPQIKLAKKLVELSGLDRAFFCNSGAEANEGAIKFARKYVSKVLGREGGEIISMYNAFHGRTLTTLAATPKPKYQDGFYPLPPGFKYVPFNDIEALKEAITDKTAAIMIEPVQGEGGIHVADKDYLKAVRDLCDDKNIVLIFDEVQCGMGRTGRMFAFEHYGVEPDILTLAKALGGGVPIGAVVLKEEIAKALSYGDHGTTFGGNPLACSAALASVEVIEELIKDDKVIEKGKYFIRKLENLIEKYNFIKEVRGLGLMIGAELEFNGADIVKKMLEKGFLINCTSDTVLRFLPPLIVEKEHIDALINALDEVFTEIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58140</accession>
    <name>Y730_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0730</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0730</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0208.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64391">
        <property type="entry name" value="B64391"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870235.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58140"/>
    <dbReference type="SMR" id="Q58140"/>
    <dbReference type="STRING" id="243232.MJ_0730"/>
    <dbReference type="EnsemblBacteria" id="AAB98726">
        <property type="protein sequence ID" value="AAB98726"/>
        <property type="gene ID" value="MJ_0730"/>
    </dbReference>
    <dbReference type="GeneID" id="1451607"/>
    <dbReference type="KEGG" id="mja:MJ_0730"/>
    <dbReference type="eggNOG" id="arCOG01705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098523_0_0_2"/>
    <dbReference type="InParanoid" id="Q58140"/>
    <dbReference type="OMA" id="IYPPDNK"/>
    <dbReference type="OrthoDB" id="72619at2157"/>
    <dbReference type="PhylomeDB" id="Q58140"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014072">
        <property type="entry name" value="Archaeoflavo_AfpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02699">
        <property type="entry name" value="archaeo_AfpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107006" description="Uncharacterized protein MJ0730">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="186" mass="21182" checksum="DE9526F02DB1B25A" modified="1996-11-01" version="1">MLKIAWGITGCGDKLPEVVEIMKKLKNKYNLDVDIYLSKNAKIVVKWYKLWQVLEDEFYDLRVEVNANAPFLVGKLQTGKYDLFLVAPATANTTAKIAYGIADTLITNSVAQAMKAKVPVYIFPPDNKKGTVETILPGNKKLTLYMRDVDVENVERLRRMEGIEVLDKPEDIEKVILKHIEVKKQQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58149</accession>
    <name>Y739_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0739</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0739</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the M.jannaschii MJ0150/MJ0739/MJ0745/MJ1460/MJ1642 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64392">
        <property type="entry name" value="C64392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870244.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58149"/>
    <dbReference type="SMR" id="Q58149"/>
    <dbReference type="STRING" id="243232.MJ_0739"/>
    <dbReference type="EnsemblBacteria" id="AAB98744">
        <property type="protein sequence ID" value="AAB98744"/>
        <property type="gene ID" value="MJ_0739"/>
    </dbReference>
    <dbReference type="GeneID" id="1451616"/>
    <dbReference type="KEGG" id="mja:MJ_0739"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128702_1_0_2"/>
    <dbReference type="InParanoid" id="Q58149"/>
    <dbReference type="OMA" id="FEANNIG"/>
    <dbReference type="OrthoDB" id="38758at2157"/>
    <dbReference type="PhylomeDB" id="Q58149"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019642">
        <property type="entry name" value="DUF2507"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10702">
        <property type="entry name" value="DUF2507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107010" description="Uncharacterized protein MJ0739">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="151" mass="17461" checksum="465891BCFEE7044D" modified="1996-11-01" version="1">MISVKDVVNYNPEEYKFKSREIPSDLLAIIIYAYMQKVKDLGSDTTLYEIGYEVGRLVSPKSYEDIKKFFEANNIGYIEIKEKDNGEVEIKVKDCIFCRTQKSEEPMCDFEAGLIAGFLESIKNKKYFVKEMYCQAQGYDACVFIAKPLHN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58162</accession>
    <name>Y752_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0752</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0752</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0857 N-terminal region.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64393">
        <property type="entry name" value="H64393"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58162"/>
    <dbReference type="STRING" id="243232.MJ_0752"/>
    <dbReference type="EnsemblBacteria" id="AAB98754">
        <property type="protein sequence ID" value="AAB98754"/>
        <property type="gene ID" value="MJ_0752"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0752"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2645918_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107014" description="Uncharacterized protein MJ0752">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="76" mass="9186" checksum="ABDC436ED6ECC4F8" modified="1996-11-01" version="1">MMEVIKAIEFKYYSDVVELIYDFKEMVNFCIDKAMELGITSYAKLRKAIYNEWKEKWYPKYHTHYCHSACRVATSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58182</accession>
    <name>Y772_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0772</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0772</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64396">
        <property type="entry name" value="D64396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870277.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58182"/>
    <dbReference type="STRING" id="243232.MJ_0772"/>
    <dbReference type="PRIDE" id="Q58182"/>
    <dbReference type="EnsemblBacteria" id="AAB98776">
        <property type="protein sequence ID" value="AAB98776"/>
        <property type="gene ID" value="MJ_0772"/>
    </dbReference>
    <dbReference type="GeneID" id="1451649"/>
    <dbReference type="KEGG" id="mja:MJ_0772"/>
    <dbReference type="eggNOG" id="arCOG05049">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800796_0_0_2"/>
    <dbReference type="OMA" id="YRAKKAW"/>
    <dbReference type="OrthoDB" id="42050at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107026" description="Uncharacterized protein MJ0772">
        <location>
            <begin position="1"/>
            <end position="353"/>
        </location>
    </feature>
    <sequence length="353" mass="42478" checksum="06843C680B4EA752" modified="1998-12-15" version="2">MKIIKAVPEEFLNGLYLIPTDRGNLLELDENGNFEVVNNIAIIRVLATPNLKKAIAKIVYQIKDKYYLFKNHKKANWLKNLLEYMNNKIEFDKYYRAKKAWYRGIGDLFRNIRKWEFEKVVGKIISLLKVLNPIVVFDVHDIRKWHYRKSFINFVKELRKNSISVVIRYPIDCHEEIREIFPEGILNTKATIRYFAKVHGYYISDRVAEYLLKITNGNLETIYLILRHSKREIKNLRELKIPWLRILPYIVDSKYRKLVEVIIELRKFKVEDITYKVNYKLSTIYRYLDELVELGILTKIKHKGKVRFKIRLNRNILLTLLKKSKNNYLHWFSIFLLDSIPWDIQIFEFSKSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58193</accession>
    <name>Y783_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0783</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0783</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64397">
        <property type="entry name" value="G64397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870289.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2B0A">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.45 A"/>
        <property type="chains" value="A=2-186"/>
    </dbReference>
    <dbReference type="PDBsum" id="2B0A"/>
    <dbReference type="AlphaFoldDB" id="Q58193"/>
    <dbReference type="SMR" id="Q58193"/>
    <dbReference type="STRING" id="243232.MJ_0783"/>
    <dbReference type="DNASU" id="1451661"/>
    <dbReference type="EnsemblBacteria" id="AAB98780">
        <property type="protein sequence ID" value="AAB98780"/>
        <property type="gene ID" value="MJ_0783"/>
    </dbReference>
    <dbReference type="GeneID" id="1451661"/>
    <dbReference type="KEGG" id="mja:MJ_0783"/>
    <dbReference type="eggNOG" id="arCOG02462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030671_3_2_2"/>
    <dbReference type="InParanoid" id="Q58193"/>
    <dbReference type="OMA" id="FKFFGFP"/>
    <dbReference type="OrthoDB" id="93116at2157"/>
    <dbReference type="PhylomeDB" id="Q58193"/>
    <dbReference type="EvolutionaryTrace" id="Q58193"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004061">
        <property type="term" value="F:arylformamidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007325">
        <property type="entry name" value="KFase/CYL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037175">
        <property type="entry name" value="KFase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31118">
        <property type="entry name" value="PTHR31118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04199">
        <property type="entry name" value="Cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102198">
        <property type="entry name" value="SSF102198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107034" description="Uncharacterized protein MJ0783">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="3"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="22"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="31"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="42"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="49"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="65"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="74"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="86"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="94"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="101"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="113"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="123"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="134"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="153"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="162"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="176"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="2B0A"/>
        </source>
    </evidence>
    <sequence length="186" mass="21238" checksum="E4A68C828AE48310" modified="1996-11-01" version="1">MEILDLTQTLINFPYPGDPELRIIEKKIDGFIVSEIIMGSHLCTHIDYPKHVGLENRIPFKDGIIKGKGYCISLDDFERNKLPACDILLIYTGFSKYWGRDEYFEKIPEIPFLDDIIKSNIKCVGIDACTIGGFEEHKRLLSNNILIIENLNENLKNLVGKSFYFLGLPLKIFDIDASPIRCIAIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58213</accession>
    <name>Y803_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0803</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0803</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0695.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64400">
        <property type="entry name" value="C64400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870313.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58213"/>
    <dbReference type="STRING" id="243232.MJ_0803"/>
    <dbReference type="EnsemblBacteria" id="AAB98816">
        <property type="protein sequence ID" value="AAB98816"/>
        <property type="gene ID" value="MJ_0803"/>
    </dbReference>
    <dbReference type="GeneID" id="1451685"/>
    <dbReference type="KEGG" id="mja:MJ_0803"/>
    <dbReference type="eggNOG" id="arCOG10950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493009_0_0_2"/>
    <dbReference type="OrthoDB" id="136947at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107053" description="Uncharacterized protein MJ0803">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="172" mass="20073" checksum="0D49E317D6518DB5" modified="1996-11-01" version="1">MNSYGVILISYVGLIKLALAGILCYGIYLAIKSEKNLIKDALFVYKDNNFNVFGKKYALMIFLAFGFPIFFIGSFLYLFWEKLPEGFRFSLTFAITFFVLFIFGLLFVKYKIRVCKNGIYVGFRFITWKGFEGYKIENNKIILIGKKGVTYPVHLKYSKELEDIIKNYLKKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58221</accession>
    <name>Y811_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0811</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0811</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64401">
        <property type="entry name" value="C64401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870322.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58221"/>
    <dbReference type="SMR" id="Q58221"/>
    <dbReference type="STRING" id="243232.MJ_0811"/>
    <dbReference type="EnsemblBacteria" id="AAB98810">
        <property type="protein sequence ID" value="AAB98810"/>
        <property type="gene ID" value="MJ_0811"/>
    </dbReference>
    <dbReference type="GeneID" id="1451694"/>
    <dbReference type="KEGG" id="mja:MJ_0811"/>
    <dbReference type="eggNOG" id="arCOG00442">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036888_0_0_2"/>
    <dbReference type="InParanoid" id="Q58221"/>
    <dbReference type="OMA" id="MTQKITW"/>
    <dbReference type="OrthoDB" id="13269at2157"/>
    <dbReference type="PhylomeDB" id="Q58221"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032781">
        <property type="term" value="P:positive regulation of ATP-dependent activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107059" description="Uncharacterized protein MJ0811">
        <location>
            <begin position="1"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="273"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00219"/>
        </source>
    </evidence>
    <sequence length="439" mass="51119" checksum="2F85F2B942C1CDFD" modified="1997-11-01" version="1">MKNIIKHDAYDKKAYERFLKNSKYLQKLISYYSQYHPIHEKLAEDTFYAFFKYVVEFNEYVEEKFKINKAILEGAIKNIEYEKSKLLTELDEVNAGTATIMFCEKFFENLKLAKLNKELKKFASEGKGEGLEDKLKEIAKNTMKDIAEEVSEVIQGFNAVENFGKGEGDKKLLSPEDRIKLADKILQNKKIREIVKKLGKLRLLAINEYKSKIKHYSGEIYSTKIGRDLKHLLPKEIVNLSDEILYYDFLRRFVDKKLLIYDIQNKLEKQKGPIIILLDHSGSMYGDREIWGKAVALSIIEIAKRENRDIYYIAFDDGVRFEKKINPKTITFDEIIEIASLYFGGGTNFIMPLNRAMSIIKEHETFKNADILLITDGYAEVNDVFLKEFDKFKNEYNAKLISVFVETFPTETLKAISDEVIKVYDLADEEARKIYKSIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58224</accession>
    <name>DHYS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable deoxyhypusine synthase</fullName>
            <shortName>DHS</shortName>
            <ecNumber>2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">dys</name>
        <name type="ordered locus">MJ0814</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98813" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.5.1.46"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98813.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64401">
        <property type="entry name" value="F64401"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58224"/>
    <dbReference type="SMR" id="Q58224"/>
    <dbReference type="STRING" id="243232.MJ_0814"/>
    <dbReference type="EnsemblBacteria" id="AAB98813">
        <property type="protein sequence ID" value="AAB98813"/>
        <property type="gene ID" value="MJ_0814"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0814"/>
    <dbReference type="eggNOG" id="arCOG04142">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039781_0_0_2"/>
    <dbReference type="InParanoid" id="Q58224"/>
    <dbReference type="OMA" id="FWSYFCQ"/>
    <dbReference type="PhylomeDB" id="Q58224"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008612">
        <property type="term" value="P:peptidyl-lysine modification to peptidyl-hypusine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00153">
        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="PTHR11703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134495" description="Probable deoxyhypusine synthase">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="330" mass="37581" checksum="FADF38ED01F5D9F9" modified="2002-10-10" version="3">MLKESEDIEGIAIEGPWLEDDISLEEIIKKYYLKIGFQASHIGKAIKIWKHIEEKRKKGDEITVFFGYTSNIVSSGLREIIAYLVKHKKIDIIVTTAGGVEEDFIKCLKPFILGDWEVDGKMLREKGINRIGNIFVPNDRYIAFEEYMMEFFEEILNLQRETGKIITASEFCYKLGEFMDKKLKSKEKEKSILYWAYKNNIPIFCPAITDGSIGDMLYFFKKYNKDEELKIDVANDIVKLNDIAINSKETACIVLGGSLPKHSIINANLFREGTDYAIYVTTALPWDGSLSGAPPEEGVSWGKIGAKADYVEIWGDATIIFPLLVYCVMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58229</accession>
    <name>Y819_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative uncharacterized protein MJ0819</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0819</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Could be the product of a pseudogene. MJ0821, MJ0820 and MJ0819 respectively represent the N-terminal, central and C-terminal sections of other members of this family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64402">
        <property type="entry name" value="C64402"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58229"/>
    <dbReference type="SMR" id="Q58229"/>
    <dbReference type="STRING" id="243232.MJ_0819"/>
    <dbReference type="EnsemblBacteria" id="AAB98831">
        <property type="protein sequence ID" value="AAB98831"/>
        <property type="gene ID" value="MJ_0819"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0819"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177888_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="uncertain"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184681" description="Putative uncharacterized protein MJ0819">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="109" mass="13065" checksum="C72C396D1BF6EC9A" modified="1996-11-01" version="1">MLVGSQLIEIVIDSLRYENLKEILDEMFRDEVQKLKYFLEDVKEEDEELYNKIVDALKLFKENYEIEDIKIPKKIRVFLVKNNILFLNPQKGSLKPQSYLVWNAIKMLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58235</accession>
    <name>FKBPL_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Long-type peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">Long-type PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">FKBP-type peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="5">FKBP-type PPIase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">FKBP26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Rotamase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0825</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference evidence="7 8 9" key="2">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="407" first="450" last="464">
            <title>Structural analysis of protein folding by the long-chain archaeal chaperone FKBP26.</title>
            <authorList>
                <person name="Martinez-Hackert E."/>
                <person name="Hendrickson W.A."/>
            </authorList>
            <dbReference type="PubMed" id="21262232"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2011.01.027"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 1-150</scope>
        <scope>FUNCTION AS A CHAPERONE</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Catalyzes the cis-trans isomerization of peptidyl prolyl bonds and accelerates protein folding (By similarity). Also exhibits chaperone-like activity (PubMed:21262232).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains an N-terminal PPIase domain, an IF (Insert in the Flap) domain and a C-terminal domain (CTD). The CTD mediates dimerization. Chaperone activity requires both the IF domain and the CTD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870336.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3PR9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=1-150"/>
    </dbReference>
    <dbReference type="PDB" id="3PRA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-150"/>
    </dbReference>
    <dbReference type="PDB" id="3PRB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-231"/>
    </dbReference>
    <dbReference type="PDB" id="3PRD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.30 A"/>
        <property type="chains" value="A=1-231"/>
    </dbReference>
    <dbReference type="PDBsum" id="3PR9"/>
    <dbReference type="PDBsum" id="3PRA"/>
    <dbReference type="PDBsum" id="3PRB"/>
    <dbReference type="PDBsum" id="3PRD"/>
    <dbReference type="AlphaFoldDB" id="Q58235"/>
    <dbReference type="SMR" id="Q58235"/>
    <dbReference type="STRING" id="243232.MJ_0825"/>
    <dbReference type="EnsemblBacteria" id="AAB98824">
        <property type="protein sequence ID" value="AAB98824"/>
        <property type="gene ID" value="MJ_0825"/>
    </dbReference>
    <dbReference type="GeneID" id="1451708"/>
    <dbReference type="KEGG" id="mja:MJ_0825"/>
    <dbReference type="eggNOG" id="arCOG00980">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073526_1_0_2"/>
    <dbReference type="InParanoid" id="Q58235"/>
    <dbReference type="OMA" id="IREFHRH"/>
    <dbReference type="OrthoDB" id="116879at2157"/>
    <dbReference type="PhylomeDB" id="Q58235"/>
    <dbReference type="EvolutionaryTrace" id="Q58235"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040825">
        <property type="entry name" value="FKBP26_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18046">
        <property type="entry name" value="FKBP26_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0697">Rotamase</keyword>
    <feature type="chain" id="PRO_0000075377" description="Long-type peptidyl-prolyl cis-trans isomerase">
        <location>
            <begin position="1"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase FKBP-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="IF" evidence="6">
        <location>
            <begin position="83"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="CTD" evidence="6">
        <location>
            <begin position="151"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="19"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="26"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="44"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="55"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="69"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="85"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="88"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="93"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="114"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="125"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="turn" evidence="10">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="139"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="152"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="171"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="178"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="187"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="helix" evidence="11">
        <location>
            <begin position="193"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="strand" evidence="11">
        <location>
            <begin position="213"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O27197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="21262232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="21262232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="21262232"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="3PR9"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="3PRA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="3PRB"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="3PR9"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="11">
        <source>
            <dbReference type="PDB" id="3PRB"/>
        </source>
    </evidence>
    <sequence length="231" mass="25947" checksum="ABD9AD1DB1B6C420" modified="1998-12-15" version="2">MVEKGKMVKISYDGYVDGKLFDTTNEELAKKEGIYNPAMIYGPVAIFAGEGQVLPGLDEAILEMDVGEEREVVLPPEKAFGKRDPSKIKLIPLSEFTKRGIKPIKGLTITIDGIPGKIVSINSGRVLVDFNHELAGKEVKYRIKIEEVVDDKKNIVKEIVKMYVPRLSDVKVTIRNGTVKIELPEFAPFIPNIQTAKMAIANEILKRLEDAEKVSFVETFERKKETKEENK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58240</accession>
    <name>Y830_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0830</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0830</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LarE family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64403">
        <property type="entry name" value="F64403"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870341.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58240"/>
    <dbReference type="SMR" id="Q58240"/>
    <dbReference type="STRING" id="243232.MJ_0830"/>
    <dbReference type="DNASU" id="1451713"/>
    <dbReference type="EnsemblBacteria" id="AAB98829">
        <property type="protein sequence ID" value="AAB98829"/>
        <property type="gene ID" value="MJ_0830"/>
    </dbReference>
    <dbReference type="GeneID" id="1451713"/>
    <dbReference type="KEGG" id="mja:MJ_0830"/>
    <dbReference type="eggNOG" id="arCOG00043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061181_0_0_2"/>
    <dbReference type="InParanoid" id="Q58240"/>
    <dbReference type="OMA" id="LVTRIPY"/>
    <dbReference type="OrthoDB" id="56165at2157"/>
    <dbReference type="PhylomeDB" id="Q58240"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01990">
        <property type="entry name" value="Alpha_ANH_like_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005232">
        <property type="entry name" value="LarE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF2">
        <property type="entry name" value="PTHR43169:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006661">
        <property type="entry name" value="PP-lp_UCP006661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00268">
        <property type="entry name" value="TIGR00268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107066" description="Uncharacterized protein MJ0830">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="252" mass="29736" checksum="2F4A13CCBEC1D470" modified="1996-11-01" version="1">MVFLKKLIKLKNNLKEKFKNKKIIIAYSGGIDSLLLSILLSEITETLCIFIKTPYISEWSLNNAIINAKKYNLNLKVIKIDKIIKNVPERCYLCKKMFFEILTKEKEKYNYDVVVDGTNYDDLFEDRPGLRAKEEFNIGSPFADFKIGKKDILEIAKELNINIPPKETCLLTRFEFNREISIEDLKKIEELEEFLRNYVKGAIRVRDYKNLAVIEIEDDLSKIINEKEEIIKKFKDYGFKKVCINLEIYRSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58249</accession>
    <name>PRIS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="1">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">priS</name>
        <name type="synonym">priA</name>
        <name type="ordered locus">MJ0839</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="Nucleic Acids Res." volume="27" first="4444" last="4450">
            <title>Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Desogus G."/>
                <person name="Onesti S."/>
                <person name="Brick P."/>
                <person name="Rossi M."/>
                <person name="Pisani F."/>
            </authorList>
            <dbReference type="PubMed" id="10536154"/>
            <dbReference type="DOI" id="10.1093/nar/27.22.4444"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1 2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 8.06.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 60-70 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870353.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58249"/>
    <dbReference type="STRING" id="243232.MJ_0839"/>
    <dbReference type="EnsemblBacteria" id="AAB98844">
        <property type="protein sequence ID" value="AAB98844"/>
        <property type="gene ID" value="MJ_0839"/>
    </dbReference>
    <dbReference type="GeneID" id="1451727"/>
    <dbReference type="KEGG" id="mja:MJ_0839"/>
    <dbReference type="eggNOG" id="arCOG04110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056123_1_0_2"/>
    <dbReference type="InParanoid" id="Q58249"/>
    <dbReference type="OMA" id="GYHVHVR"/>
    <dbReference type="OrthoDB" id="95578at2157"/>
    <dbReference type="PhylomeDB" id="Q58249"/>
    <dbReference type="BRENDA" id="2.7.7.102">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="2.7.7.B16">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003896">
        <property type="term" value="F:DNA primase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of RNA primer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="PTHR10536"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0639">Primosome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000046742" description="DNA primase small subunit PriS">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10536154"/>
        </source>
    </evidence>
    <sequence length="350" mass="41802" checksum="D308EBA1FB23EEFD" modified="1998-12-15" version="2">MNTFAEVQKLYREYYNFAIKNNILEIPEGIEYREFGYGYLKKVDNRNLSFKNEREYKDWVLKNAPMHFYKSLAYMLYPNKSGGASKKGIFRRELAFDIDVHKTKKCKHEDDWICKHCLEEAKNQAIYLIEEFLIPDFGLNEEDLKIVFSGNRGYHIYIKPRDEKIRDIIESYSKEDRRFLMDYILGKNLNLNSVGSGWRRRLIKAIKERDKRISTKKLENEKNWKKVIENLKSKNKIYNIIEETKNKIELDEKVMDDDIRLLRVINSLHGYTGFIVKPLSGLDELRRFNPLEDAIFKDFENKVYEVNIFDDRKFEIEICGKKYNNKSKKITASALLYLFGHNIKFELLKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58253</accession>
    <name>MCRD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase I operon protein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mcrD</name>
        <name type="ordered locus">MJ0843</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">MCR is composed of three subunits: alpha, beta, and gamma. The function of proteins C and D is not known.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64405">
        <property type="entry name" value="C64405"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58253"/>
    <dbReference type="STRING" id="243232.MJ_0843"/>
    <dbReference type="EnsemblBacteria" id="AAB98848">
        <property type="protein sequence ID" value="AAB98848"/>
        <property type="gene ID" value="MJ_0843"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0843"/>
    <dbReference type="eggNOG" id="arCOG04859">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118415_0_0_2"/>
    <dbReference type="InParanoid" id="Q58253"/>
    <dbReference type="OMA" id="ELEVFPH"/>
    <dbReference type="PhylomeDB" id="Q58253"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003901">
        <property type="entry name" value="Me_CoM_Rdtase_D"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02505">
        <property type="entry name" value="MCR_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005636">
        <property type="entry name" value="McrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03260">
        <property type="entry name" value="met_CoM_red_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147494" description="Methyl-coenzyme M reductase I operon protein D">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <sequence length="164" mass="19048" checksum="CC68B5A87ADDB174" modified="1996-11-01" version="1">MFMIEVEIFPHRYLKASTTEKFLNKIYDLKTVERVVIHGQPLPKVITYGPARGLPVNHTERKIIHVKGVPVELTVMAGRFWITLSDDSELDKLDEICKEMFPFGYNLRVGKFLKDRPTVTDYIKYGEDGVFLINEMDRRLIGMVDPRSRMANSVTVVEKEEKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q58266</accession>
    <name>Y856_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0856</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0856</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64406">
        <property type="entry name" value="H64406"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58266"/>
    <dbReference type="SMR" id="Q58266"/>
    <dbReference type="STRING" id="243232.MJ_0856"/>
    <dbReference type="EnsemblBacteria" id="AAB98860">
        <property type="protein sequence ID" value="AAB98860"/>
        <property type="gene ID" value="MJ_0856"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0856"/>
    <dbReference type="eggNOG" id="arCOG04906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529229_0_0_2"/>
    <dbReference type="OMA" id="QWVVLSE"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017098">
        <property type="entry name" value="UCP037052"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09869">
        <property type="entry name" value="DUF2096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037052">
        <property type="entry name" value="UCP037052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107080" description="Uncharacterized protein MJ0856">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <sequence length="181" mass="21124" checksum="DBB6B58BCD8F9F56" modified="1996-11-01" version="1">MEMGNGMKDARNLDKQWVVLSELSAELVNRGIKVPEIVFEKLRLANALLSYYILDPHASINILANVERELNYVQSQLFSLCDTELTEKYLDRMIKAIRGEINAKFPVSKSNYNREVKKRGKVEAIRVKLQKEMQIERLSDLGEWHGVIFEYSDEKDKVIIEGNIDRVKRALKDFAFMWKED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q58285</accession>
    <name>Y875_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0875</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0875</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64409">
        <property type="entry name" value="C64409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870390.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58285"/>
    <dbReference type="SMR" id="Q58285"/>
    <dbReference type="STRING" id="243232.MJ_0875"/>
    <dbReference type="PRIDE" id="Q58285"/>
    <dbReference type="EnsemblBacteria" id="AAB98882">
        <property type="protein sequence ID" value="AAB98882"/>
        <property type="gene ID" value="MJ_0875"/>
    </dbReference>
    <dbReference type="GeneID" id="1451764"/>
    <dbReference type="KEGG" id="mja:MJ_0875"/>
    <dbReference type="eggNOG" id="arCOG03045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_371589_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107088" description="Uncharacterized protein MJ0875">
        <location>
            <begin position="1"/>
            <end position="748"/>
        </location>
    </feature>
    <sequence length="748" mass="90732" checksum="5515ED3B541562EA" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58322</accession>
    <name>Y912_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0912</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0912</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64413">
        <property type="entry name" value="H64413"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58322"/>
    <dbReference type="SMR" id="Q58322"/>
    <dbReference type="STRING" id="243232.MJ_0912"/>
    <dbReference type="EnsemblBacteria" id="AAB98917">
        <property type="protein sequence ID" value="AAB98917"/>
        <property type="gene ID" value="MJ_0912"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0912"/>
    <dbReference type="eggNOG" id="arCOG01143">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074761_0_1_2"/>
    <dbReference type="InParanoid" id="Q58322"/>
    <dbReference type="OMA" id="DEYLFPW"/>
    <dbReference type="PhylomeDB" id="Q58322"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011152">
        <property type="entry name" value="Pesterase_MJ0912"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000883">
        <property type="entry name" value="Pesterase_MJ0912"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107101" description="Uncharacterized protein MJ0912">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <sequence length="243" mass="27819" checksum="98B27C4E23E6C136" modified="1996-11-01" version="1">MVKSMIAVISDIHSNLEALNAVLNDIKNRGIKKIFCLGDIVGYGANPNECVELIRDLNCLSVVGNHDYGVLGKESLDYFNKYGAIAILWTKKVIKNENLKFLDSLPLIIEENIKGKKVIFSHANPKHPEIWEYLFPDYVDDVFNYGDLIFVGHSHIPFVNSEEGNLLLHEGKIYLDEDKKYLINPGSVGQPRDGINKASYCIFDEKDFKIEIVRVEYDIKKAYEKIVKNRLPEWLGERLFLGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q58325</accession>
    <name>ASPD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L-aspartate dehydrogenase</fullName>
            <ecNumber evidence="1">1.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadX</name>
        <name type="ordered locus">MJ0915</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-aspartate + NADP(+) = H(+) + NADPH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11784"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-aspartate + NAD(+) = H(+) + NADH + NH4(+) + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:11788"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.21" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64414">
        <property type="entry name" value="C64414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870429.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58325"/>
    <dbReference type="SMR" id="Q58325"/>
    <dbReference type="STRING" id="243232.MJ_0915"/>
    <dbReference type="EnsemblBacteria" id="AAB98920">
        <property type="protein sequence ID" value="AAB98920"/>
        <property type="gene ID" value="MJ_0915"/>
    </dbReference>
    <dbReference type="GeneID" id="1451804"/>
    <dbReference type="KEGG" id="mja:MJ_0915"/>
    <dbReference type="eggNOG" id="arCOG00254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089550_0_0_2"/>
    <dbReference type="InParanoid" id="Q58325"/>
    <dbReference type="OMA" id="ECAGHSA"/>
    <dbReference type="OrthoDB" id="50102at2157"/>
    <dbReference type="PhylomeDB" id="Q58325"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00456"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01265">
        <property type="entry name" value="NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022487">
        <property type="entry name" value="Asp_DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020626">
        <property type="entry name" value="Asp_DH_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011182">
        <property type="entry name" value="L-Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="DUF108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005227">
        <property type="entry name" value="Asp_dh_NAD_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03855">
        <property type="entry name" value="NAD_NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000144896" description="L-aspartate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01265"/>
        </source>
    </evidence>
    <sequence length="267" mass="28755" checksum="B2C427DFD3AE1A4F" modified="1996-11-01" version="1">MLKIGIVGCGAIGNFITKKVLDGTIKNAKISAVYDRNFDKAKTLSERTGAKICSSIDDLVKEDLDLVVEAASIKAVEEIAEKSLINNKDVLIMSVGALADKKLFLKLRDLAKTVGRKIYLPSGAIGGLDAIKALRLGEIEEVVLKTTKPVAALEDALKNLGYKPEDIKNPVIVFEGDVFKAIKEFPANINVSVTLSIAAEFPAKVVIVADPNAKLNKHELFVKSSIGTLRVCIENVPFEENPRTSALAAYSAVRLIRDLAEPVKVGT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58331</accession>
    <name>Y921_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0921</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0921</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64415">
        <property type="entry name" value="A64415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870435.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58331"/>
    <dbReference type="SMR" id="Q58331"/>
    <dbReference type="STRING" id="243232.MJ_0921"/>
    <dbReference type="EnsemblBacteria" id="AAB98925">
        <property type="protein sequence ID" value="AAB98925"/>
        <property type="gene ID" value="MJ_0921"/>
    </dbReference>
    <dbReference type="GeneID" id="1451810"/>
    <dbReference type="KEGG" id="mja:MJ_0921"/>
    <dbReference type="eggNOG" id="arCOG01116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065511_0_0_2"/>
    <dbReference type="InParanoid" id="Q58331"/>
    <dbReference type="OMA" id="NKTQNCV"/>
    <dbReference type="OrthoDB" id="67932at2157"/>
    <dbReference type="PhylomeDB" id="Q58331"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="DUF1743"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017674">
        <property type="entry name" value="Methan_mark_11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="DUF1743"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03280">
        <property type="entry name" value="methan_mark_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107106" description="Uncharacterized protein MJ0921">
        <location>
            <begin position="1"/>
            <end position="300"/>
        </location>
    </feature>
    <sequence length="300" mass="33031" checksum="601DB0F8F1BF822C" modified="1996-11-01" version="1">MSVNYKSVIAMVDDALNLVEIVEEHPCPNGSEWVIYQYQRTSPLILSAWREGNKHHFVTKIGKEKLNLVPSLSAAGIEEVYIENNRVHIVYAGLAGGGVGAELRKGAKNVLEVNILEKGGGSRLGKAEVITPKMEKVIIGIDDTDTKEEGATWVLAHEIGLEVEKEGLGYYLDHTIVQLYPGNPNKTQNCVSIALSFAVYPEYKYKLDKFIKKLLKERSLSDETAMAVYYGLFPSKSMKLFALKAKKEMVKIEEAKSIALRNNIKIIPINGEGGIIGAVAALGLAEHHSLAPKLCEDIKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58338</accession>
    <name>Y928_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative protein N5-glutamine methyltransferase MJ0928</fullName>
            <ecNumber evidence="2">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>M.MjaHemkP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0928</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative protein methyltransferase using S-adenosyl-L-methionine as the methyl donor. May methylate a Gln residue in target proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-glutaminyl-[protein] + S-adenosyl-L-methionine = H(+) + N(5)-methyl-L-glutaminyl-[protein] + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:57452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:14895"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61891"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64415">
        <property type="entry name" value="H64415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870442.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58338"/>
    <dbReference type="SMR" id="Q58338"/>
    <dbReference type="STRING" id="243232.MJ_0928"/>
    <dbReference type="EnsemblBacteria" id="AAB98930">
        <property type="protein sequence ID" value="AAB98930"/>
        <property type="gene ID" value="MJ_0928"/>
    </dbReference>
    <dbReference type="GeneID" id="1451817"/>
    <dbReference type="KEGG" id="mja:MJ_0928"/>
    <dbReference type="eggNOG" id="arCOG00109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018398_6_2_2"/>
    <dbReference type="InParanoid" id="Q58338"/>
    <dbReference type="OMA" id="EWDDWME"/>
    <dbReference type="OrthoDB" id="72076at2157"/>
    <dbReference type="PhylomeDB" id="Q58338"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000157177" description="Putative protein N5-glutamine methyltransferase MJ0928">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="197" mass="22057" checksum="2AE2377FE86EB217" modified="1996-11-01" version="1">MIIEIEGIKLKLHPEVYEPAEDSILLLKNLVDVKNKDVLEIGVGTGLISIACAKKGAKKIVGVDINPYAVKLAKENAKLNNVNISFFESDLFENVTGKFDVILFNPPYLPTSEDEKIDSYLNFAFDGGKDGREILDRFIYELPNYLKKGGVVQILQSSLTGEKETINKLKPLGFKVEISARLKVPFEELMVINAWRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58371</accession>
    <name>Y961_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized MCM-type protein MJ0961</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0961</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64420">
        <property type="entry name" value="A64420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870475.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58371"/>
    <dbReference type="SMR" id="Q58371"/>
    <dbReference type="STRING" id="243232.MJ_0961"/>
    <dbReference type="EnsemblBacteria" id="AAB98963">
        <property type="protein sequence ID" value="AAB98963"/>
        <property type="gene ID" value="MJ_0961"/>
    </dbReference>
    <dbReference type="GeneID" id="1451859"/>
    <dbReference type="KEGG" id="mja:MJ_0961"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_2"/>
    <dbReference type="InParanoid" id="Q58371"/>
    <dbReference type="OMA" id="MHAKARL"/>
    <dbReference type="OrthoDB" id="3067at2157"/>
    <dbReference type="PhylomeDB" id="Q58371"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000194130" description="Uncharacterized MCM-type protein MJ0961">
        <location>
            <begin position="1"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="domain" description="MCM">
        <location>
            <begin position="334"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="384"/>
            <end position="391"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="762" mass="87526" checksum="3323509057464C12" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58377</accession>
    <name>Y967_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0967</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0967</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64420">
        <property type="entry name" value="G64420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870481.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58377"/>
    <dbReference type="SMR" id="Q58377"/>
    <dbReference type="STRING" id="243232.MJ_0967"/>
    <dbReference type="EnsemblBacteria" id="AAB98972">
        <property type="protein sequence ID" value="AAB98972"/>
        <property type="gene ID" value="MJ_0967"/>
    </dbReference>
    <dbReference type="GeneID" id="1451865"/>
    <dbReference type="KEGG" id="mja:MJ_0967"/>
    <dbReference type="eggNOG" id="arCOG00769">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_44_4_2"/>
    <dbReference type="InParanoid" id="Q58377"/>
    <dbReference type="OMA" id="IGKYGVP"/>
    <dbReference type="OrthoDB" id="98884at2157"/>
    <dbReference type="PhylomeDB" id="Q58377"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000157847" description="Uncharacterized protein MJ0967">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="205" mass="22542" checksum="E60E6AD066ECCFB5" modified="1996-11-01" version="1">MKRLAVILITLALVSSMCITNSNEKRENMKNAKVLMVIAPKDFRDEELFEPMAVFESNGLKVDVVSTTKGECVGMLGNKITVEKTIYDVNPDDYVAIVIVGGIGSKEYLWNNTKLIELVKEFYNKNKVVSAICLSPVVLARAGILKGKKATVYPAPEAIEELKKAGAIYEDRGVVVDGNVITAKSPDYARLFGLEVLKAIEKNNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58383</accession>
    <name>Y973_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0973</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0973</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the PAPS reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64421">
        <property type="entry name" value="E64421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870487.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58383"/>
    <dbReference type="SMR" id="Q58383"/>
    <dbReference type="STRING" id="243232.MJ_0973"/>
    <dbReference type="PRIDE" id="Q58383"/>
    <dbReference type="EnsemblBacteria" id="AAB98978">
        <property type="protein sequence ID" value="AAB98978"/>
        <property type="gene ID" value="MJ_0973"/>
    </dbReference>
    <dbReference type="GeneID" id="1451871"/>
    <dbReference type="KEGG" id="mja:MJ_0973"/>
    <dbReference type="eggNOG" id="arCOG00074">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026622_0_0_2"/>
    <dbReference type="InParanoid" id="Q58383"/>
    <dbReference type="OMA" id="FERIGCY"/>
    <dbReference type="OrthoDB" id="31010at2157"/>
    <dbReference type="PhylomeDB" id="Q58383"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="PAPS_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100694" description="Uncharacterized protein MJ0973">
        <location>
            <begin position="1"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="411" mass="48904" checksum="E823355794A38F94" modified="1997-11-01" version="1">MDDKFASKFEIDVLNKLLNKNFSYDLAIILKKIGGLDYRKKVFINGECIGILEFDLIDLDWKFHPYASYYLIEEPKIKIKPTKRKLKGKKVPVDLIENAEELKDINENDYVGVEVGNYVGVAVKKGDTIKIKDLTLKKELRFEKIEDYLRKNKDRIEKLEKKSLSIIKKYYEMCKNKNYAINTSFSGGKDSSVSTLLANKVIDDLEVIFIDTGLEFKDTIDFVKKFAKKYDLNLVVLKGKNFWEYLEKEGIPTKDYRWCNSVCKLEPLKEYLKKYKRVYTIDGSRRYESFTREKLTYERKSGFIENQINIFPILDWRGTDVWSWIYLNDVIYNELYDKGFERIGCYMCPAALNAEFLRVKELYPELFNKWVDVLKRFGYDEDEILRGFWRWKELPPKMKELKKILENKEKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58392</accession>
    <name>MTM1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II methyltransferase M.MjaI</fullName>
            <shortName evidence="1">M.MjaI</shortName>
            <ecNumber>2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N-4 cytosine-specific methyltransferase MjaI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaIM</name>
        <name type="ordered locus">MJ0985</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="patent" date="1999-03-11" number="WO9911821">
            <title>Method for screening restriction endonucleases.</title>
            <authorList>
                <person name="Noren C.J."/>
                <person name="Roberts R.J."/>
                <person name="Patti J."/>
                <person name="Byrd D.R."/>
                <person name="Morgan R.D."/>
            </authorList>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">A beta subtype methylase that recognizes the double-stranded sequence 5'-CTAG-3', methylates C-1 on both strands, and protects the DNA from cleavage by the MjaI endonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64423">
        <property type="entry name" value="A64423"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870499.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58392"/>
    <dbReference type="SMR" id="Q58392"/>
    <dbReference type="STRING" id="243232.MJ_0985"/>
    <dbReference type="REBASE" id="3889">
        <property type="entry name" value="M.MjaI"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98988">
        <property type="protein sequence ID" value="AAB98988"/>
        <property type="gene ID" value="MJ_0985"/>
    </dbReference>
    <dbReference type="GeneID" id="1451883"/>
    <dbReference type="KEGG" id="mja:MJ_0985"/>
    <dbReference type="eggNOG" id="arCOG00115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_2_2_2"/>
    <dbReference type="InParanoid" id="Q58392"/>
    <dbReference type="OMA" id="MIEMWDD"/>
    <dbReference type="OrthoDB" id="63009at2157"/>
    <dbReference type="PhylomeDB" id="Q58392"/>
    <dbReference type="PRO" id="PR:Q58392"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000087936" description="Type II methyltransferase M.MjaI">
        <location>
            <begin position="1"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0000305" key="3">
        <source ref="2"/>
    </evidence>
    <sequence length="303" mass="35518" checksum="6D4DB9B36EA90E80" modified="1996-11-01" version="1">MMSIDITTNHKIIFGDARKMDEIEDESVHLVVTSPPYPMIEMWDELFKMLNLEINKRWMEMENEEDEEKKEKLIMQIYNLMHQTLYPVWEEVYRVLVPGGIACINIGDATRKINGVFRLFPNHSKIIENFEKIGFVTLPYILWKKPSNKPNAFLGSGFLPPNAYVTLDVEYILIFRKGKPRKFKPKDPLRYASAYTKEERDRWFSQIWEIVGDKQTHPKIERRTASFPEEIPRRLIRMFSIIGDTVLDPFLGTGTTVKAAIELKRNSIGYEIDKSLKPIIEEKIGIKQKRIGMDFNVEFINRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q58497</accession>
    <name>DCDA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="1">DAP decarboxylase</shortName>
            <shortName evidence="1">DAPDC</shortName>
            <ecNumber evidence="1 2">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lysA</name>
        <name type="ordered locus">MJ1097</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Structure" volume="10" first="1499" last="1508">
            <title>Cocrystal structures of diaminopimelate decarboxylase: mechanism, evolution, and inhibition of an antibiotic resistance accessory factor.</title>
            <authorList>
                <person name="Ray S.S."/>
                <person name="Bonanno J.B."/>
                <person name="Rajashankar K.R."/>
                <person name="Pinho M.G."/>
                <person name="He G."/>
                <person name="De Lencastre H."/>
                <person name="Tomasz A."/>
                <person name="Burley S.K."/>
            </authorList>
            <dbReference type="PubMed" id="12429091"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(02)00880-8"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 5-438 IN COMPLEXES WITH PLP; L-LYSINE AND SUBSTRATE ANALOG</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + meso-2,6-diaminoheptanedioate = CO2 + L-lysine</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Competitively inhibited by the substrate analog azelaic acid in vitro but not in vivo.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">588 uM for meso-2,6-diaminoheptanedioate</KM>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64436">
        <property type="entry name" value="H64436"/>
    </dbReference>
    <dbReference type="PDB" id="1TUF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=5-438"/>
    </dbReference>
    <dbReference type="PDB" id="1TWI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D=6-438"/>
    </dbReference>
    <dbReference type="PDBsum" id="1TUF"/>
    <dbReference type="PDBsum" id="1TWI"/>
    <dbReference type="AlphaFoldDB" id="Q58497"/>
    <dbReference type="SMR" id="Q58497"/>
    <dbReference type="STRING" id="243232.MJ_1097"/>
    <dbReference type="EnsemblBacteria" id="AAB99100">
        <property type="protein sequence ID" value="AAB99100"/>
        <property type="gene ID" value="MJ_1097"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1097"/>
    <dbReference type="eggNOG" id="arCOG02268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026444_0_1_2"/>
    <dbReference type="InParanoid" id="Q58497"/>
    <dbReference type="OMA" id="YCRSMAS"/>
    <dbReference type="PhylomeDB" id="Q58497"/>
    <dbReference type="SABIO-RK" id="Q58497"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58497"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
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            <dbReference type="PubMed" id="12429091"/>
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</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58518</accession>
    <name>Y1118_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ1118</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1118</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
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            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
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    <proteinExistence type="predicted"/>
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</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58523</accession>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ1123</fullName>
        </recommendedName>
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        <name type="ordered locus">MJ1123</name>
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        </lineage>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0638 and MJ1252 and M.tuberculosis Rv2003c.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58523"/>
    <dbReference type="SMR" id="Q58523"/>
    <dbReference type="STRING" id="243232.MJ_1123"/>
    <dbReference type="EnsemblBacteria" id="AAB99125">
        <property type="protein sequence ID" value="AAB99125"/>
        <property type="gene ID" value="MJ_1123"/>
    </dbReference>
    <dbReference type="GeneID" id="1452019"/>
    <dbReference type="KEGG" id="mja:MJ_1123"/>
    <dbReference type="eggNOG" id="arCOG01773">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037990_14_1_2"/>
    <dbReference type="InParanoid" id="Q58523"/>
    <dbReference type="OMA" id="CIVPRES"/>
    <dbReference type="OrthoDB" id="45753at2157"/>
    <dbReference type="PhylomeDB" id="Q58523"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107178" description="Uncharacterized protein MJ1123">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="205" mass="23560" checksum="21791B51CEA3E2B6" modified="1996-11-01" version="1">MNVFDKYAEEYDKWFDENEIIYKSEIEALKRHIPKGRGLEIGVGTGRFAKPFNIKIGVDISKEMAKIAEKRGIKVIIAKGEDLPFKDEEFDFFLINTVLEFAENPKKMIEEAKRVLKRGGKIIIGIIDRDSFLGKMYEEKKQKSKFYKDANFLSAKEVIEMLKELGFKNIKATQTIFKEIDKVDKVEVKEGYGEGGFVAISAEKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58569</accession>
    <name>FWDA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Protein FwdA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fwdA</name>
        <name type="ordered locus">MJ1169</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. FwdA/FmdA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64445">
        <property type="entry name" value="H64445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870682.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58569"/>
    <dbReference type="SMR" id="Q58569"/>
    <dbReference type="STRING" id="243232.MJ_1169"/>
    <dbReference type="DNASU" id="1452067"/>
    <dbReference type="EnsemblBacteria" id="AAB99171">
        <property type="protein sequence ID" value="AAB99171"/>
        <property type="gene ID" value="MJ_1169"/>
    </dbReference>
    <dbReference type="GeneID" id="1452067"/>
    <dbReference type="KEGG" id="mja:MJ_1169"/>
    <dbReference type="eggNOG" id="arCOG04461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035587_0_0_2"/>
    <dbReference type="InParanoid" id="Q58569"/>
    <dbReference type="OMA" id="GEKMDIC"/>
    <dbReference type="OrthoDB" id="8152at2157"/>
    <dbReference type="PhylomeDB" id="Q58569"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01304">
        <property type="entry name" value="FMDH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012027">
        <property type="entry name" value="Formylmethanofuran_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006453">
        <property type="entry name" value="FwdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03121">
        <property type="entry name" value="one_C_dehyd_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000087391" description="Protein FwdA">
        <location>
            <begin position="1"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="567" mass="63411" checksum="EB6B1A37DFB4737C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q58597</accession>
    <name>SYT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 5">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="1">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">thrS</name>
        <name type="ordered locus">MJ1197</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Proc. Natl. Acad. Sci. U.S.A." volume="101" first="10260" last="10265">
            <title>A freestanding proofreading domain is required for protein synthesis quality control in Archaea.</title>
            <authorList>
                <person name="Korencic D."/>
                <person name="Ahel I."/>
                <person name="Schelert J."/>
                <person name="Sacher M."/>
                <person name="Ruan B."/>
                <person name="Stathopoulos C."/>
                <person name="Blum P."/>
                <person name="Ibba M."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="15240874"/>
            <dbReference type="DOI" id="10.1073/pnas.0403926101"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF HIS-7; PHE-13; ASP-46; HIS-80; LYS-118; CYS-124; HIS-127 AND ARG-134</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="J. Biol. Chem." volume="280" first="571" last="577">
            <title>A unique hydrophobic cluster near the active site contributes to differences in borrelidin inhibition among threonyl-tRNA synthetases.</title>
            <authorList>
                <person name="Ruan B."/>
                <person name="Bovee M.L."/>
                <person name="Sacher M."/>
                <person name="Stathopoulos C."/>
                <person name="Poralla K."/>
                <person name="Francklyn C.S."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="15507440"/>
            <dbReference type="DOI" id="10.1074/jbc.M411039200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference evidence="8 9" key="4">
        <citation type="journal article" date="2015" name="Nat. Commun." volume="6" first="7552" last="7552">
            <title>Specificity and catalysis hardwired at the RNA-protein interface in a translational proofreading enzyme.</title>
            <authorList>
                <person name="Ahmad S."/>
                <person name="Muthukumar S."/>
                <person name="Kuncha S.K."/>
                <person name="Routh S.B."/>
                <person name="Yerabham A.S."/>
                <person name="Hussain T."/>
                <person name="Kamarthapu V."/>
                <person name="Kruparani S.P."/>
                <person name="Sankaranarayanan R."/>
            </authorList>
            <dbReference type="PubMed" id="26113036"/>
            <dbReference type="DOI" id="10.1038/ncomms8552"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 1-141 (EDITING DOMAIN) IN COMPLEX WITH POST-TRANSFER EDITING SUBSTRATE ANALOGS</scope>
        <scope>FUNCTION</scope>
        <scope>EDITING REACTION MECHANISM</scope>
        <scope>MUTAGENESIS OF TYR-117; LYS-118 AND GLU-131</scope>
    </reference>
    <comment type="function">
        <text evidence="2 4">Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: L-threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (PubMed:15240874). Also activates L-serine, but does not detectably transfer it to tRNA(Thr) (PubMed:15240874). Edits incorrectly charged L-seryl-tRNA(Thr) via its editing domain (PubMed:15240874). Has no activity on correctly acylated L-seryl-tRNA(Ser) or L-threonyl-tRNA(Thr) (PubMed:15240874). Deacylates correctly charged glycyl-tRNA(Gly), but not glycyl-tRNA(Gly)(2'-dA76) (the terminal 2'-OH of tRNA adenine 76 has been dehydroxylated) nor the 2'-fluoro tRNA derivative, strongly suggesting the editing function is tRNA catalyzed (PubMed:26113036).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-threonyl-tRNA(Thr)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Not inhibited by 1 uM borrelidin (BN); probably does not bind BN.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">25 mM for L-serine activation</KM>
            <KM evidence="2 3">100 mM for L-threonine activation</KM>
            <text evidence="2 3">kcat is 12.3 sec(-1) for L-serine, 13.5 for L-threonine at 60 degrees Celsius (PubMed:15240874). kcat is 1.2 sec(-1) (PubMed:15507440).</text>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="2 4">The N-terminal domain (about residues 1-140) is an archaea-specific tRNA-editing domain (PubMed:15240874, PubMed:26113036) that has a highly similar structure to Dtd (D-aminoacyl-tRNA deacylase). Editing of incorrectly charged L-seryl-tRNA(Thr) by this domain is tRNA catalyzed (PubMed:26113036).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="6">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99201" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99201.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64449">
        <property type="entry name" value="D64449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496752.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4RRF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-141"/>
    </dbReference>
    <dbReference type="PDB" id="4RRG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.93 A"/>
        <property type="chains" value="A/B/C/D=1-141"/>
    </dbReference>
    <dbReference type="PDBsum" id="4RRF"/>
    <dbReference type="PDBsum" id="4RRG"/>
    <dbReference type="AlphaFoldDB" id="Q58597"/>
    <dbReference type="SMR" id="Q58597"/>
    <dbReference type="STRING" id="243232.MJ_1197"/>
    <dbReference type="PRIDE" id="Q58597"/>
    <dbReference type="DNASU" id="1452092"/>
    <dbReference type="EnsemblBacteria" id="AAB99201">
        <property type="protein sequence ID" value="AAB99201"/>
        <property type="gene ID" value="MJ_1197"/>
    </dbReference>
    <dbReference type="GeneID" id="1452092"/>
    <dbReference type="KEGG" id="mja:MJ_1197"/>
    <dbReference type="eggNOG" id="arCOG00401">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029833_0_0_2"/>
    <dbReference type="InParanoid" id="Q58597"/>
    <dbReference type="OMA" id="IATFQID"/>
    <dbReference type="OrthoDB" id="11656at2157"/>
    <dbReference type="PhylomeDB" id="Q58597"/>
    <dbReference type="BRENDA" id="6.1.1.3">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00771">
        <property type="entry name" value="ThrRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015011">
        <property type="entry name" value="Threonyl-tRNA_syn_edit_dom_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033728">
        <property type="entry name" value="ThrRS_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="PTHR11451"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08915">
        <property type="entry name" value="tRNA-Thr_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000101101" description="Threonine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Editing domain" evidence="1 7">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Catalytic" evidence="1">
        <location>
            <begin position="197"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="465"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Still charges Thr, no longer edits mischarged L-seryl-tRNA(Thr)." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="7"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Still charges Thr, 5% editing of mischarged L-seryl-tRNA(Thr)." evidence="2">
        <original>F</original>
        <variation>A</variation>
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Wild-type for Thr charging and L-seryl-tRNA(Thr) editing." evidence="2">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Still charges Thr, no longer edits mishcarged L-seryl-tRNA(Thr), high mischarging activity." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Isolated domain has nearly wild-type deacylation of mischarged L-seryl-tRNA(Thr), no activity on L-threonyl-tRNA(Thr). Intermediate deacylation of L-seryl-tRNA(Thr); when associated with A-131." evidence="4">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Still charges Thr, no longer edits mischarged L-seryl-tRNA(Thr)." evidence="2">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Isolated domain does not deacylate mischarged L-seryl-tRNA(Thr), no activity on L-threonyl-tRNA(Thr)." evidence="4">
        <original>K</original>
        <variation>M</variation>
        <location>
            <position position="118"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Still charges Thr, 80% editing of mischarged L-seryl-tRNA(Thr), no mischarging activity." evidence="2">
        <original>C</original>
        <variation>A</variation>
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Still charges Thr, no longer edits mischarged L-seryl-tRNA(Thr), high mischarging activity." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="127"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Isolated domain has almost no deacylation of mischarged L-seryl-tRNA(Thr), no activity on L-threonyl-tRNA(Thr). Intermediate deacylation of L-seryl-tRNA(Thr); when associated with A-117." evidence="4">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Still charges Thr, 20% editing of mischarged L-seryl-tRNA(Thr)." evidence="2">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="2"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="44"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="72"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="116"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="130"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="15507440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="26113036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="15240874"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="4RRF"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="9">
        <source>
            <dbReference type="PDB" id="4RRG"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="4RRF"/>
        </source>
    </evidence>
    <sequence length="620" mass="72357" checksum="514D190E790F64F6" modified="2000-05-30" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58617</accession>
    <name>T1M2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Type I restriction enzyme MjaVIII methylase subunit</fullName>
            <shortName>M protein</shortName>
            <ecNumber evidence="1">2.1.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Type I methyltransferase M.MjaVIII</fullName>
            <shortName evidence="3">M.MjaVIII</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1220</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">The subtype gamma methyltransferase (M) subunit of a type I restriction enzyme. The M and S subunits together form a methyltransferase (MTase) that methylates A-2 on the top and A-3 on the bottom strand of the sequence 5'-GAYN(5)GTAA-3'. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg(2+) as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64452">
        <property type="entry name" value="C64452"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870732.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1220"/>
    <dbReference type="REBASE" id="182832">
        <property type="entry name" value="M.Bli37I"/>
    </dbReference>
    <dbReference type="REBASE" id="3905">
        <property type="entry name" value="M.MjaVIII"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q58617"/>
    <dbReference type="EnsemblBacteria" id="AAB99225">
        <property type="protein sequence ID" value="AAB99225"/>
        <property type="gene ID" value="MJ_1220"/>
    </dbReference>
    <dbReference type="GeneID" id="1452116"/>
    <dbReference type="KEGG" id="mja:MJ_1220"/>
    <dbReference type="eggNOG" id="arCOG02632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013049_4_2_2"/>
    <dbReference type="InParanoid" id="Q58617"/>
    <dbReference type="OMA" id="NSADWAW"/>
    <dbReference type="OrthoDB" id="45297at2157"/>
    <dbReference type="PhylomeDB" id="Q58617"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000088001" description="Type I restriction enzyme MjaVIII methylase subunit">
        <location>
            <begin position="1"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="250"/>
            <end position="255"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="280"/>
            <end position="282"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="332"/>
            <end position="333"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P08957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q89Z59"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="578" mass="66638" checksum="5339ED873EF8E9E2" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-16" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58646</accession>
    <name>DHQS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>3-dehydroquinate synthase</fullName>
            <shortName>DHQ synthase</shortName>
            <ecNumber>1.4.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>3-dehydroquinate synthase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">aroB'</name>
        <name type="ordered locus">MJ1249</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="Biochemistry" volume="43" first="7618" last="7627">
            <title>L-aspartate semialdehyde and a 6-deoxy-5-ketohexose 1-phosphate are the precursors to the aromatic amino acids in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="15182204"/>
            <dbReference type="DOI" id="10.1021/bi0495127"/>
        </citation>
        <scope>FUNCTION AS A DHQ SYNTHASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidative deamination and cyclization of 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid (ADH) to yield 3-dehydroquinate (DHQ), which is fed into the canonical shikimic pathway of aromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate + H2O + NAD(+) = 3-dehydroquinate + H(+) + NADH + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:25956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="EC" id="1.4.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal-type DHQ synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.24"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64455">
        <property type="entry name" value="H64455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870761.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58646"/>
    <dbReference type="SMR" id="Q58646"/>
    <dbReference type="STRING" id="243232.MJ_1249"/>
    <dbReference type="DNASU" id="1452146"/>
    <dbReference type="EnsemblBacteria" id="AAB99252">
        <property type="protein sequence ID" value="AAB99252"/>
        <property type="gene ID" value="MJ_1249"/>
    </dbReference>
    <dbReference type="GeneID" id="1452146"/>
    <dbReference type="KEGG" id="mja:MJ_1249"/>
    <dbReference type="eggNOG" id="arCOG04353">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056379_0_0_2"/>
    <dbReference type="InParanoid" id="Q58646"/>
    <dbReference type="OMA" id="HFGMAIK"/>
    <dbReference type="OrthoDB" id="29853at2157"/>
    <dbReference type="PhylomeDB" id="Q58646"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14595"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102042">
        <property type="term" value="F:dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01244">
        <property type="entry name" value="Arch_DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002812">
        <property type="entry name" value="DHQ_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563">
        <property type="entry name" value="PTHR33563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01959">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006655">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000058769" description="3-dehydroquinate synthase">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="15182204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="361" mass="40260" checksum="8A072D5A4712D7FE" modified="1996-11-01" version="1">MKFGWVNVIGDNWEEKKKIVTTALESSIPVVVAEPEDIEKIKELGNIKVASHSLDADIVLVNKNDNIEFLKEAKNLGKETAIYIPIESKEDEEFASEVARFGFVDNIILEGRDWTIIPLENLIADLFHRDVKIVASVNSVDEAKVAYEILEKGTDGVLLNPKNLEDIKELSKLIEEMNKEKVALDVATVTKVEPIGSGDRVCIDTCSLMKIGEGMLIGSYSRALFLVHSETVENPYVATRPFRVNAGPVHAYILCPGNKTKYLSELKAGDKVLIVDKDGNTREAIVGRVKIERRPLVLIEAEYKGDIIRTILQNAETIRLVNEKGEPISVVDLKPGDKVLIKPEEYARHFGMAIKETIIEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q58674</accession>
    <name>Y1278_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1278</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1278</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64459">
        <property type="entry name" value="E64459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870791.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58674"/>
    <dbReference type="SMR" id="Q58674"/>
    <dbReference type="STRING" id="243232.MJ_1278"/>
    <dbReference type="EnsemblBacteria" id="AAB99284">
        <property type="protein sequence ID" value="AAB99284"/>
        <property type="gene ID" value="MJ_1278"/>
    </dbReference>
    <dbReference type="GeneID" id="1452176"/>
    <dbReference type="KEGG" id="mja:MJ_1278"/>
    <dbReference type="eggNOG" id="arCOG10917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500292_0_0_2"/>
    <dbReference type="OMA" id="HILRITV"/>
    <dbReference type="OrthoDB" id="113317at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107246" description="Uncharacterized protein MJ1278">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="139"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="179" mass="20662" checksum="CBC0BA276E7EC1A0" modified="1996-11-01" version="1">MGIFDLAKKITHSREYTKSIDEIFVGELINFMYKNGAVLTEINSPTESSHSLTFKFVNHPVLHILRITVDRKIEGMASKILGSQSVLTFEAVIKNDLVEPNDVLVMYQTDFKNMFKIPIFGKVKINHDLNYIIATTTYIEDLGKYIKSDRIEKEALREELEKILNTLVKHLEPLKKKFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58679</accession>
    <name>Y1283_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1283</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1283</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64460">
        <property type="entry name" value="B64460"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58679"/>
    <dbReference type="STRING" id="243232.MJ_1283"/>
    <dbReference type="EnsemblBacteria" id="AAB99292">
        <property type="protein sequence ID" value="AAB99292"/>
        <property type="gene ID" value="MJ_1283"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1283"/>
    <dbReference type="eggNOG" id="arCOG08268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253591_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107252" description="Uncharacterized protein MJ1283">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="220" mass="25277" checksum="037E6FD391C703B9" modified="1996-11-01" version="1">MVEMNKRGQFFIIGGVILSIGLILFFLLGFNSYTSDGSYLTVFKMKDVKNSIESCLINSLTSNSNLSKNLDMLKNNYKDEGIEINYKKIIFSNIRYEAKNLTFNFSLYNGNFSYNISNYGFGGAFNGSLNVSNYVFSKNLLLNISENGSVTGSFNITGSYVNVFVYDRFGNLILNETIYNNSNEKSLYYYILNVSKEGILLYLLWQRMFLTTHWQKMYPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58717</accession>
    <name>AGO_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein argonaute</fullName>
            <shortName evidence="6">MjAgo</shortName>
            <ecNumber evidence="2">3.1.25.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1321</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="RNA Biol." volume="11" first="45" last="56">
            <title>Single-molecule FRET supports the two-state model of Argonaute action.</title>
            <authorList>
                <person name="Zander A."/>
                <person name="Holzmeister P."/>
                <person name="Klose D."/>
                <person name="Tinnefeld P."/>
                <person name="Grohmann D."/>
            </authorList>
            <dbReference type="PubMed" id="24442234"/>
            <dbReference type="DOI" id="10.4161/rna.27446"/>
        </citation>
        <scope>FUNCTION AS AN ENDONUCLEASE</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>DOMAIN</scope>
        <scope>DNA-BINDING</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2016" name="PLoS ONE" volume="11" first="E0164695" last="E0164695">
            <title>Mechanistic insights into archaeal and human Argonaute substrate binding and cleavage properties.</title>
            <authorList>
                <person name="Willkomm S."/>
                <person name="Zander A."/>
                <person name="Grohmann D."/>
                <person name="Restle T."/>
            </authorList>
            <dbReference type="PubMed" id="27741323"/>
            <dbReference type="DOI" id="10.1371/journal.pone.0164695"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBSTRATE AND TARGET BINDING</scope>
        <scope>MUTAGENESIS OF TYR-442</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2017" name="Nat. Microbiol." volume="2" first="17034" last="17034">
            <title>Guide-independent DNA cleavage by archaeal Argonaute from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Zander A."/>
                <person name="Willkomm S."/>
                <person name="Ofer S."/>
                <person name="van Wolferen M."/>
                <person name="Egert L."/>
                <person name="Buchmeier S."/>
                <person name="Stoeckl S."/>
                <person name="Tinnefeld P."/>
                <person name="Schneider S."/>
                <person name="Klingl A."/>
                <person name="Albers S.V."/>
                <person name="Werner F."/>
                <person name="Grohmann D."/>
            </authorList>
            <dbReference type="PubMed" id="28319081"/>
            <dbReference type="DOI" id="10.1038/nmicrobiol.2017.34"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INDUCTION</scope>
        <scope>MUTAGENESIS OF TYR-194; HIS-213; TYR-217; GLU-246; ASP-438; LYS-483 AND GLU-541</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference evidence="11 12" key="5">
        <citation type="journal article" date="2017" name="Nat. Microbiol." volume="2" first="17035" last="17035">
            <title>Structural and mechanistic insights into an archaeal DNA-guided Argonaute protein.</title>
            <authorList>
                <person name="Willkomm S."/>
                <person name="Oellig C.A."/>
                <person name="Zander A."/>
                <person name="Restle T."/>
                <person name="Keegan R."/>
                <person name="Grohmann D."/>
                <person name="Schneider S."/>
            </authorList>
            <dbReference type="PubMed" id="28319084"/>
            <dbReference type="DOI" id="10.1038/nmicrobiol.2017.35"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.29 ANGSTROMS) IN COMPLEX WITH METAL WITH AND WITHOUT GUIDE DNA</scope>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
        <scope>DOMAIN</scope>
        <scope>MUTAGENESIS OF TYR-194; HIS-213; TYR-217; GLU-246; LEU-270; TRP-274; LYS-435; ASP-438; GLN-457; ASN-458; GLN-479; LYS-483; PHE-572; GLN-574 AND ASN-575</scope>
        <scope>DNA-BINDING</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3 4 5 9">An endodeoxyribonuclease that may play a role in defense against invading genetic elements. Uses short DNA sequences as guides to bind complementary target strands, resulting in slicing of the target DNA (PubMed:24442234, PubMed:27741323, PubMed:28319081, PubMed:28319084). Also has guide-independent activity on target DNA (PubMed:28319081). Probably a first round of guide-independent activity on an invading plasmid or virus would generate guide DNAs for subsequent, more efficient, guide-dependent degradation of invading nucleic acids (PubMed:28319081). Endonucleolytically cleaves DNA in short dsDNA (the guide DNA indicates where to cleave on the target DNA) (PubMed:24442234, PubMed:27741323, PubMed:28319081, PubMed:28319084). Efficient guide-dependent target DNA cleavage requires a minimal length of 15 bp and is maximal at 19 bp (PubMed:28319081). Prefers guide DNA with 5'-phosphorylated purines and 3'-pyrimidines; changing these bases alters the cleavage activity and patterns (PubMed:28319084). Has no activity on substrate with a mismatch at positions 10 and 11, ssDNA or RNA, nor on DNA-RNA hybrids (PubMed:24442234). Digests longer (750 bp) dsDNA as well as circular plasmid and naked genomic DNA, but not chromatin, in a guide DNA-independent manner (PubMed:28319081). Addition of endogenous histone A3 protects DNA from cleavage, while cleavage is insensitive to methylation (PubMed:28319081). When plasmid encoding active or mutated protein (Ala-541) is transformed into Sulfolobus acidocaldarius about 25-fold fewer transformants are found with active protein; reduced levels of plasmid are found in wild-type transformed cells. While S.acidocaldarius grows at a similar temperature to M.jannaschii (70 to 80 degrees Celsius) it has very different histone-like proteins, which presumably do not protect against MjAgo (PubMed:28319081). Binds ssDNA, dsDNA and DNA-RNA hybrids; binding is most efficient with dsDNA (PubMed:24442234).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="5">Also liganded by DNA (PubMed:28319084).</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2 4">DNA cleavage is inhibited by EDTA.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="2">Optimum temperature is over 75 degrees Celsius for DNA cleavage, DNA-binding occurs below that.</text>
        </temperatureDependence>
    </comment>
    <comment type="induction">
        <text evidence="9">Constitutively expressed (at protein level) (PubMed:28319081).</text>
    </comment>
    <comment type="domain">
        <text evidence="5 8">Has 4 structurally distinct domains; N-terminal, PAZ (binds the 3'-end of guide DNA), Mid (binds the phosphorylated 5'-end of guide DNA) and PIWI (binds near the 5'-end of guide DNA) arranged in a bilobal manner (PubMed:28319084). Upon binding of guide DNA the PAZ and Mid domains separate, opening a channel probably for target DNA-binding; a smaller channel also opens between the N-terminal and PIWI domains (PubMed:28319084). The N-terminal and PAZ domains undergo further major rearrangement when the binary Ago-guide DNA complex binds target DNA; the PAZ domain binds the 3'-end of guide DNA in the absence of target, upon ternary complex formation it is probably the N-terminal domain that binds that end of the dsDNA (PubMed:24442234).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the argonaute family. Long pAgo subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.25.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64464">
        <property type="entry name" value="H64464"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870838.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="5G5S">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.29 A"/>
        <property type="chains" value="A=1-713"/>
    </dbReference>
    <dbReference type="PDB" id="5G5T">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.85 A"/>
        <property type="chains" value="A=1-713"/>
    </dbReference>
    <dbReference type="PDBsum" id="5G5S"/>
    <dbReference type="PDBsum" id="5G5T"/>
    <dbReference type="AlphaFoldDB" id="Q58717"/>
    <dbReference type="SMR" id="Q58717"/>
    <dbReference type="STRING" id="243232.MJ_1321"/>
    <dbReference type="PRIDE" id="Q58717"/>
    <dbReference type="EnsemblBacteria" id="AAB99334">
        <property type="protein sequence ID" value="AAB99334"/>
        <property type="gene ID" value="MJ_1321"/>
    </dbReference>
    <dbReference type="GeneID" id="1452223"/>
    <dbReference type="KEGG" id="mja:MJ_1321"/>
    <dbReference type="eggNOG" id="arCOG03890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_362759_0_0_2"/>
    <dbReference type="OMA" id="NILWENW"/>
    <dbReference type="OrthoDB" id="11100at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="SSF101690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107275" description="Protein argonaute">
        <location>
            <begin position="1"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="1 5 8">
        <location>
            <begin position="426"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal structural domain" evidence="5 8">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="PAZ structural domain" evidence="5 8">
        <location>
            <begin position="163"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Binds 3'-end of guide DNA" evidence="5 12">
        <location>
            <begin position="213"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Mid structural domain" evidence="5 8">
        <location>
            <begin position="346"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Binds 5'-phosphorylated end of guide DNA" evidence="5 12">
        <location>
            <begin position="457"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Binds 5'-phosphorylated end of guide DNA" evidence="5 12">
        <location>
            <begin position="625"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Binds 5'-phosphorylated end of guide DNA" evidence="5 12">
        <location>
            <begin position="678"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="457"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="483"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="site" description="Interacts with guide DNA" evidence="5 12">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with guide DNA" evidence="5 12">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with guide DNA" evidence="5 12">
        <location>
            <position position="191"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with 3'-end of guide DNA" evidence="5 12">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with guide DNA" evidence="5 12">
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with guide DNA" evidence="5 12">
        <location>
            <position position="479"/>
        </location>
    </feature>
    <feature type="site" description="Interacts with guide DNA" evidence="5 12">
        <location>
            <position position="669"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No guide-independent plasmid cleavage. Loss of guide-dependent cleavage of target DNA." evidence="4 5">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Significantly reduced guide-independent plasmid cleavage. Decreased guide-dependent cleavage of target DNA." evidence="4 5">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Significantly reduced guide-independent plasmid cleavage. Decreased guide-dependent cleavage of target DNA." evidence="4 5">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Nearly complete loss of guide-independent plasmid cleavage. Loss of guide-dependent cleavage of target DNA." evidence="4 5">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of guide-dependent cleavage of target DNA when guide DNA starts with 5'-dT but not 5'-dG." evidence="5">
        <original>L</original>
        <variation>P</variation>
        <location>
            <position position="270"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No change in guide-dependent cleavage of target DNA." evidence="5">
        <original>W</original>
        <variation>V</variation>
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Wild-type guide-dependent cleavage of target DNA." evidence="5">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="435"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No guide-independent plasmid cleavage. Decreased guide-dependent cleavage of target DNA." evidence="4 5">
        <original>D</original>
        <variation>P</variation>
        <location>
            <position position="438"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Alters binding kinetics of guide DNA, probably due to loss of Mid domain binding to 5'-end of guide." evidence="4">
        <original>Y</original>
        <variation>A</variation>
        <location>
            <position position="442"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of guide-dependent cleavage of target DNA." evidence="5">
        <original>Q</original>
        <variation>A</variation>
        <location>
            <position position="457"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of guide-dependent cleavage of target DNA." evidence="5">
        <original>N</original>
        <variation>A</variation>
        <location>
            <position position="458"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Decreased guide-dependent cleavage of target DNA." evidence="5">
        <original>Q</original>
        <variation>A</variation>
        <location>
            <position position="479"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No guide-independent plasmid cleavage. Loss of guide-dependent cleavage of target DNA." evidence="4 5">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="483"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No guide-dependent or -independent cleavage of target DNA." evidence="4">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="541"/>
        </location>
    </feature>
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    <accession>Q58727</accession>
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            <fullName>Acylphosphatase-like protein MJ1331</fullName>
        </recommendedName>
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
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            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58731</accession>
    <name>GMHA_METJA</name>
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        <name type="synonym">Methanococcus jannaschii</name>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
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            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27489"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:60203"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="EC" id="5.3.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. GmhA subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAB99342" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="5.3.1.28" evidence="1"/>
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        <property type="protein sequence ID" value="AAB99342.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64466">
        <property type="entry name" value="F64466"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58731"/>
    <dbReference type="SMR" id="Q58731"/>
    <dbReference type="STRING" id="243232.MJ_1335"/>
    <dbReference type="EnsemblBacteria" id="AAB99342">
        <property type="protein sequence ID" value="AAB99342"/>
        <property type="gene ID" value="MJ_1335"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1335"/>
    <dbReference type="eggNOG" id="arCOG05355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080999_4_1_2"/>
    <dbReference type="InParanoid" id="Q58731"/>
    <dbReference type="OMA" id="DVHICVP"/>
    <dbReference type="PhylomeDB" id="Q58731"/>
    <dbReference type="UniPathway" id="UPA00041">
        <property type="reaction ID" value="UER00436"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008968">
        <property type="term" value="F:D-sedoheptulose 7-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001061">
        <property type="term" value="P:D-glycero-D-manno-heptose 7-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05006">
        <property type="entry name" value="SIS_GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00067">
        <property type="entry name" value="GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035461">
        <property type="entry name" value="GmhA/DiaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004515">
        <property type="entry name" value="Phosphoheptose_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13580">
        <property type="entry name" value="SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00441">
        <property type="entry name" value="gmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000136552" description="Probable phosphoheptose isomerase">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="1">
        <location>
            <begin position="32"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="89"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="115"/>
            <end position="117"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="187" mass="20399" checksum="38440D7520757DB4" modified="2000-05-30" version="2">MIMKKYFEESANVKLKFIEEDEEKLKKAIEVIYNALKNGNKILICGNGGSAANSQHFAAEIVGRFKLERKGLPAIALTTDISILTAIGNDYGFDRIFERQVEALGKEGDVLAGISTSGNSENVIKAANKAKEMGIYTIGLLGKGGGKLKDIVDLALVVPSNDTARIQECHLTIYHVICEEVEKKLVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58794</accession>
    <name>Y1399_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0200 protein MJ1399</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1399</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0200 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64474">
        <property type="entry name" value="F64474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870916.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58794"/>
    <dbReference type="SMR" id="Q58794"/>
    <dbReference type="STRING" id="243232.MJ_1399"/>
    <dbReference type="EnsemblBacteria" id="AAB99408">
        <property type="protein sequence ID" value="AAB99408"/>
        <property type="gene ID" value="MJ_1399"/>
    </dbReference>
    <dbReference type="GeneID" id="1452302"/>
    <dbReference type="KEGG" id="mja:MJ_1399"/>
    <dbReference type="eggNOG" id="arCOG01045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096329_1_0_2"/>
    <dbReference type="InParanoid" id="Q58794"/>
    <dbReference type="OMA" id="DYMIVNE"/>
    <dbReference type="OrthoDB" id="101213at2157"/>
    <dbReference type="PhylomeDB" id="Q58794"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01111">
        <property type="entry name" value="UPF0200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022970">
        <property type="entry name" value="NTP_hydrolase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000094525" description="UPF0200 protein MJ1399">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01111"/>
        </source>
    </evidence>
    <sequence length="197" mass="22630" checksum="A440F8259920F873" modified="1996-11-01" version="1">MLLIGITGMPGAGKSSAYEIAKKYNLPIVSMGDVVRYETKKRGLELTPENVGNTAIKLREEFGNEAIAVACLKYIEENLKDKEIVIVEGIRSLYEVNYFRKHKPLVLIAIHSSPLTRFERLKKRGREDDSANWEVFVERDLRELGFSIGHAIALADFVVVNEKSFEDCLNQLDNILQEILNNLEKYKKYNFIYETLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58830</accession>
    <name>Y1435_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1435</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1435</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64479">
        <property type="entry name" value="B64479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870953.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2QTX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-71"/>
    </dbReference>
    <dbReference type="PDB" id="4X9C">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-71"/>
    </dbReference>
    <dbReference type="PDB" id="4X9D">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-71"/>
    </dbReference>
    <dbReference type="PDB" id="5DY9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/L=1-71"/>
    </dbReference>
    <dbReference type="PDBsum" id="2QTX"/>
    <dbReference type="PDBsum" id="4X9C"/>
    <dbReference type="PDBsum" id="4X9D"/>
    <dbReference type="PDBsum" id="5DY9"/>
    <dbReference type="AlphaFoldDB" id="Q58830"/>
    <dbReference type="SMR" id="Q58830"/>
    <dbReference type="STRING" id="243232.MJ_1435"/>
    <dbReference type="EnsemblBacteria" id="AAB99450">
        <property type="protein sequence ID" value="AAB99450"/>
        <property type="gene ID" value="MJ_1435"/>
    </dbReference>
    <dbReference type="GeneID" id="1452339"/>
    <dbReference type="KEGG" id="mja:MJ_1435"/>
    <dbReference type="eggNOG" id="arCOG08275">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2730360_0_0_2"/>
    <dbReference type="OMA" id="VTGVSNY"/>
    <dbReference type="OrthoDB" id="122525at2157"/>
    <dbReference type="EvolutionaryTrace" id="Q58830"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107326" description="Uncharacterized protein MJ1435">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="20"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="37"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="57"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="63"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="66"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="4X9C"/>
        </source>
    </evidence>
    <sequence length="71" mass="8295" checksum="AC1B6AF9651500EB" modified="1996-11-01" version="1">MNKPVKKQQPKKVIPNFEYARRLNGKKVKIFLRNGEVLDAEVTGVSNYEIMVKVGDRNLLVFKHAIDYIEY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-12-05" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q58839</accession>
    <name>FEN_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Flap endonuclease 1</fullName>
            <shortName evidence="1">FEN-1</shortName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fen</name>
        <name type="ordered locus">MJ1444</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="J. Bacteriol." volume="180" first="5406" last="5412">
            <title>Methanococcus jannaschii flap endonuclease: expression, purification, and substrate requirements.</title>
            <authorList>
                <person name="Rao H.G."/>
                <person name="Rosenfeld A."/>
                <person name="Wetmur J.G."/>
            </authorList>
            <dbReference type="PubMed" id="9765572"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1999" name="Mol. Cells" volume="9" first="45" last="48">
            <title>Expression, purification, characterization and crystallization of flap endonuclease-1 from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bae K.W."/>
                <person name="Baek K.W."/>
                <person name="Cho C.S."/>
                <person name="Hwang K.Y."/>
                <person name="Kim H.-R."/>
                <person name="Sung H.-C."/>
                <person name="Cho Y."/>
            </authorList>
            <dbReference type="PubMed" id="10102570"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="1998" name="Nat. Struct. Biol." volume="5" first="707" last="713">
            <title>The crystal structure of flap endonuclease-1 from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Hwang K.Y."/>
                <person name="Baek K."/>
                <person name="Kim H.-Y."/>
                <person name="Cho Y."/>
            </authorList>
            <dbReference type="PubMed" id="9699635"/>
            <dbReference type="DOI" id="10.1038/1406"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 6-7.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text>Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64480">
        <property type="entry name" value="C64480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870964.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1A76">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-326"/>
    </dbReference>
    <dbReference type="PDB" id="1A77">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-326"/>
    </dbReference>
    <dbReference type="PDBsum" id="1A76"/>
    <dbReference type="PDBsum" id="1A77"/>
    <dbReference type="AlphaFoldDB" id="Q58839"/>
    <dbReference type="SMR" id="Q58839"/>
    <dbReference type="STRING" id="243232.MJ_1444"/>
    <dbReference type="EnsemblBacteria" id="AAB99454">
        <property type="protein sequence ID" value="AAB99454"/>
        <property type="gene ID" value="MJ_1444"/>
    </dbReference>
    <dbReference type="GeneID" id="1452348"/>
    <dbReference type="KEGG" id="mja:MJ_1444"/>
    <dbReference type="eggNOG" id="arCOG04050">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032444_0_0_2"/>
    <dbReference type="InParanoid" id="Q58839"/>
    <dbReference type="OMA" id="GSQDYDS"/>
    <dbReference type="OrthoDB" id="41214at2157"/>
    <dbReference type="PhylomeDB" id="Q58839"/>
    <dbReference type="BRENDA" id="3.1.99.B1">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58839"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_structure-sp_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="PTHR11081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="SSF47807"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154053" description="Flap endonuclease 1">
        <location>
            <begin position="1"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="I-domain">
        <location>
            <begin position="116"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA" evidence="1">
        <location>
            <begin position="317"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 4">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 3 4">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 3 4">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="6"/>
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        </location>
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    <feature type="helix" evidence="5">
        <location>
            <begin position="17"/>
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    <feature type="strand" evidence="5">
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    <feature type="helix" evidence="5">
        <location>
            <begin position="56"/>
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    </feature>
    <feature type="strand" evidence="5">
        <location>
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    </feature>
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        <location>
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    </feature>
    <feature type="helix" evidence="5">
        <location>
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    <feature type="helix" evidence="5">
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    <feature type="strand" evidence="5">
        <location>
            <begin position="146"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="165"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="175"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="182"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="196"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="200"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="227"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="235"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="247"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="258"/>
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        </location>
    </feature>
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        <location>
            <begin position="283"/>
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        </location>
    </feature>
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    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="298"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="9699635"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="3">
        <source>
            <dbReference type="PDB" id="1A76"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="4">
        <source>
            <dbReference type="PDB" id="1A77"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1A76"/>
        </source>
    </evidence>
    <sequence length="326" mass="37527" checksum="311427F2B4B67580" modified="1996-11-01" version="1">MGVQFGDFIPKNIISFEDLKGKKVAIDGMNALYQFLTSIRLRDGSPLRNRKGEITSAYNGVFYKTIHLLENDITPIWVFDGEPPKLKEKTRKVRREMKEKAELKMKEAIKKEDFEEAAKYAKRVSYLTPKMVENCKYLLSLMGIPYVEAPSEGEAQASYMAKKGDVWAVVSQDYDALLYGAPRVVRNLTTTKEMPELIELNEVLEDLRISLDDLIDIAIFMGTDYNPGGVKGIGFKRAYELVRSGVAKDVLKKEVEYYDEIKRIFKEPKVTDNYSLSLKLPDKEGIIKFLVDENDFNYDRVKKHVDKLYNLIANKTKQKTLDAWFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58878</accession>
    <name>Y1483_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1483</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1483</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64485">
        <property type="entry name" value="B64485"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58878"/>
    <dbReference type="SMR" id="Q58878"/>
    <dbReference type="STRING" id="243232.MJ_1483"/>
    <dbReference type="EnsemblBacteria" id="AAB99498">
        <property type="protein sequence ID" value="AAB99498"/>
        <property type="gene ID" value="MJ_1483"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1483"/>
    <dbReference type="HOGENOM" id="CLU_1718224_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107369" description="Uncharacterized protein MJ1483">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="152" mass="17659" checksum="90227132448E8802" modified="1996-11-01" version="1">MINMDKTLSVIVFLISLIIIFGIYFSSSNFSFKKVEINKSTINDFGDENILTFVPEVCDRFYYEKDGFDWERNINNIETVIIGSDAVVIEKGDFNETEILKELIENGYSVESCRGVYYYKNEKALSDRYIIVGNNLIIKANDISGIRWRYLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58881</accession>
    <name>PURT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Formate-dependent phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="1">6.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5'-phosphoribosylglycinamide transformylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Formate-dependent GAR transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">GAR transformylase 2</fullName>
            <shortName evidence="1">GART 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-folate glycinamide ribonucleotide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylglycinamide formyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purT</name>
        <name type="ordered locus">MJ1486</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + formate + N(1)-(5-phospho-beta-D-ribosyl)glycinamide = ADP + H(+) + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24829"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24830"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99496" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.1.21" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99496.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64485">
        <property type="entry name" value="E64485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496844.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58881"/>
    <dbReference type="SMR" id="Q58881"/>
    <dbReference type="STRING" id="243232.MJ_1486"/>
    <dbReference type="EnsemblBacteria" id="AAB99496">
        <property type="protein sequence ID" value="AAB99496"/>
        <property type="gene ID" value="MJ_1486"/>
    </dbReference>
    <dbReference type="GeneID" id="1452392"/>
    <dbReference type="KEGG" id="mja:MJ_1486"/>
    <dbReference type="eggNOG" id="arCOG01598">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011534_1_3_2"/>
    <dbReference type="InParanoid" id="Q58881"/>
    <dbReference type="OMA" id="GMVTMIT"/>
    <dbReference type="OrthoDB" id="57699at2157"/>
    <dbReference type="PhylomeDB" id="Q58881"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14618"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043815">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase 2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01643">
        <property type="entry name" value="PurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005862">
        <property type="entry name" value="PurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01142">
        <property type="entry name" value="purT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000074959" description="Formate-dependent phosphoribosylglycinamide formyltransferase">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="1">
        <location>
            <begin position="118"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="159"/>
            <end position="164"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="194"/>
            <end position="197"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="360"/>
            <end position="361"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="389" mass="43048" checksum="3887A7EC474BECE8" modified="2008-02-26" version="2">MAIGTPLLKGSIKFLLLGSGELGKEVVIEAQRLGIECIAVDRYQNAPAMQVAHKSYVIDMKDYDALMAIIEREEPDYIVPEIEAINTDALIDAEKMGYTVIPTAEATKITMNRELIRRLAAEKLGLKTAKYEFADSLEELRDAVEKLGLPCVVKPIMSSSGKGQSVVRSEEDIEKAWKIAKEGARGIGNRVIVEEFINFDYEITLLTARTAEGTKFCEPIGHVQIDGDYHESWQPHNMSAELKEQAQDIAKKVTDALGGYGIFGVELFVKGDEVIFSEVSPRPHDTGMVTMITQEMSEFEIHVRAILGLPVSTKLIHPGASHVIKAEINKYAPKYHIEDALKVPNTKLRLFGKPNAKVGRRMGVALAYADSVEKARELAEKCAHAVRIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58882</accession>
    <name>Y1487_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized methyltransferase MJ1487</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1487</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="4">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64485">
        <property type="entry name" value="F64485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871009.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58882"/>
    <dbReference type="SMR" id="Q58882"/>
    <dbReference type="STRING" id="243232.MJ_1487"/>
    <dbReference type="EnsemblBacteria" id="AAB99497">
        <property type="protein sequence ID" value="AAB99497"/>
        <property type="gene ID" value="MJ_1487"/>
    </dbReference>
    <dbReference type="GeneID" id="1452393"/>
    <dbReference type="KEGG" id="mja:MJ_1487"/>
    <dbReference type="eggNOG" id="arCOG01356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042889_0_0_2"/>
    <dbReference type="InParanoid" id="Q58882"/>
    <dbReference type="OMA" id="AHYFMPL"/>
    <dbReference type="OrthoDB" id="90547at2157"/>
    <dbReference type="PhylomeDB" id="Q58882"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023980">
        <property type="entry name" value="CHP04013_B12-bd/rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04013">
        <property type="entry name" value="B12_SAM_MJ_1487"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51332">
        <property type="entry name" value="B12_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107371" description="Uncharacterized methyltransferase MJ1487">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="B12-binding" evidence="2">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="169"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="426" mass="48962" checksum="44C59FD3C9397351" modified="1996-11-01" version="1">MEENTALVVYYTKLHKNSFNALIGALEIDEYFDNLPIYFANKKDIFNLKEVLKKYDKVVIAISFFTTELWKTYELINELKAKYQSYRNKIIYLAGGPHPTGDPKGTLKLGFDVVCIGEGEETFPEFIKAVNEDEDYKKVKGIAYLNDNEFIYTGRRKPVDLNKYPPFPVKHNKFGHIEITRGCPYKCYFCQTPRIFGKKIRHRDVENIYKYVEIMAERNLKDIRFITPNAFGYGSKDGKTLNIDKIEKLLESIREILGNNGRIFFGTFPSEVRPEHVNIETVDLILRYADNKNLVIGAQSGSEKVLELCHRGHTVEDVYNAVRVARKAGLGVDVDFIFGLPGETEEDVEKTIKVMKDLIKMGAKIHAHTFMPLPQTPFAKANPGKVDKKIIRAMRYEIPKGIFHGTWHNQEIIAQKISKYLKTGKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58911</accession>
    <name>Y1516_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1516</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1516</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64489">
        <property type="entry name" value="C64489"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58911"/>
    <dbReference type="STRING" id="243232.MJ_1516"/>
    <dbReference type="EnsemblBacteria" id="AAB99536">
        <property type="protein sequence ID" value="AAB99536"/>
        <property type="gene ID" value="MJ_1516"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1516"/>
    <dbReference type="eggNOG" id="arCOG03293">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2285050_0_0_2"/>
    <dbReference type="InParanoid" id="Q58911"/>
    <dbReference type="OMA" id="RIVYYEQ"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007829">
        <property type="entry name" value="TM2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05154">
        <property type="entry name" value="TM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107387" description="Uncharacterized protein MJ1516">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="99" mass="11483" checksum="B837A92FA94002FB" modified="1996-11-01" version="1">MMAMNEIELMQIKDFVKDMDKNQRIVYYEQKKKSVGIAVLLSFIIPGAGQMYLGRVGKGIILLLTCWLIIPWIYSIYDAYKSAKDYNAQLYSIIFSKDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58912</accession>
    <name>Y1517_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1517</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1517</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64489">
        <property type="entry name" value="D64489"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58912"/>
    <dbReference type="STRING" id="243232.MJ_1517"/>
    <dbReference type="EnsemblBacteria" id="AAB99548">
        <property type="protein sequence ID" value="AAB99548"/>
        <property type="gene ID" value="MJ_1517"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1517"/>
    <dbReference type="eggNOG" id="arCOG14686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_999698_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107389" description="Uncharacterized protein MJ1517">
        <location>
            <begin position="1"/>
            <end position="278"/>
        </location>
    </feature>
    <sequence length="278" mass="32685" checksum="800A0DA2A0A6D022" modified="1996-11-01" version="1">MVKLMNLWSERIKDREVVEVIGCERVPLNETLNEIVVLFDEYTKRGDYKVEIFDDVFNYYHNRILTNDEFIDGVARIKLGLDTRNHAKEWLLEVVNWFAGNRYNLRDEEIKLGNRLRDWMNIGVSLVIPKELKPKFDEFYKMAKKFDWQVEIRNEMIFKTTLNGKEVYTFILMGDVVNERLDNILDGGGVFDLGKRVVCDDEWFSGWLVRDIDVNKNIMEVLGMLSLKSQEILDISVESALIRRKNIKTKSMVVKLVKVKNLIQLLKRKKKSGLNCLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58932</accession>
    <name>Y1537_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1537</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1537</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64491">
        <property type="entry name" value="H64491"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58932"/>
    <dbReference type="STRING" id="243232.MJ_1537"/>
    <dbReference type="EnsemblBacteria" id="AAB99559">
        <property type="protein sequence ID" value="AAB99559"/>
        <property type="gene ID" value="MJ_1537"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1537"/>
    <dbReference type="eggNOG" id="arCOG05089">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830572_0_0_2"/>
    <dbReference type="OMA" id="IETYEYA"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107395" description="Uncharacterized protein MJ1537">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <sequence length="343" mass="40505" checksum="839521737D113FD6" modified="1996-11-01" version="1">MWHNQGDAMSLSMNLKLCNYHNCNCNIGEEYYNHTYPQFWNRIIEKYKLNKIISYDFTSLPYYRFVGMVGDFISKNITTGPCLLTPKEIRKLNPNIDFEEVKKMFLRHPTFEDYVSVAIETNKGYKNHIIIETYEYAKLVEYKTNIPFEEALKLTKLSAKNFKKYYKKKVKAKYYLTHKKSFDRRLRELCNEHYKYYLENANISKKGKEIIKNNPEESTWLRIKVSFLPEAINKDDSTIVEPVSSIEGMLLANKISEVSGIVVRSPPTLNLKPIMNEGNENEIFYLNNDIEKEIKKLTYRTRTKWGCSLYHNLLFLNSPICCNKNCEECLEIFINKIKILKMG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q58948</accession>
    <name>Y1553_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ1553</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1553</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64493">
        <property type="entry name" value="H64493"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871077.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58948"/>
    <dbReference type="SMR" id="Q58948"/>
    <dbReference type="STRING" id="243232.MJ_1553"/>
    <dbReference type="EnsemblBacteria" id="AAB99573">
        <property type="protein sequence ID" value="AAB99573"/>
        <property type="gene ID" value="MJ_1553"/>
    </dbReference>
    <dbReference type="GeneID" id="1452461"/>
    <dbReference type="KEGG" id="mja:MJ_1553"/>
    <dbReference type="eggNOG" id="arCOG01683">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_127461_1_0_2"/>
    <dbReference type="InParanoid" id="Q58948"/>
    <dbReference type="OMA" id="WVYYKIT"/>
    <dbReference type="OrthoDB" id="143795at2157"/>
    <dbReference type="PhylomeDB" id="Q58948"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000160636" description="Uncharacterized HTH-type transcriptional regulator MJ1553">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="157" mass="18563" checksum="30F558E964AF8294" modified="1996-11-01" version="1">MKFLFSKTKIEILKKLNERNYTISELSKILGKSKSTISEHLNALYEMGLVDKENYSKWVYYKITNKGKKVLENLEALILMVSSIFALIGLWIYYIFKQIKYQAREVVLSKTVVERGIYTTYKGSTTFQKEPLFLIFLIISTFLIIFIVYLVYKIIRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58952</accession>
    <name>TYW2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>MjTYW2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA wyosine derivatives biosynthesis protein Taw2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">taw2</name>
        <name type="ordered locus">MJ1557</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2010" name="Mol. Biol. Evol." volume="27" first="2062" last="2077">
            <title>Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea.</title>
            <authorList>
                <person name="de Crecy-Lagard V."/>
                <person name="Brochier-Armanet C."/>
                <person name="Urbonavicius J."/>
                <person name="Fernandez B."/>
                <person name="Phillips G."/>
                <person name="Lyons B."/>
                <person name="Noma A."/>
                <person name="Alvarez S."/>
                <person name="Droogmans L."/>
                <person name="Armengaud J."/>
                <person name="Grosjean H."/>
            </authorList>
            <dbReference type="PubMed" id="20382657"/>
            <dbReference type="DOI" id="10.1093/molbev/msq096"/>
        </citation>
        <scope>GENE NAME</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Proc. Natl. Acad. Sci. U.S.A." volume="106" first="15616" last="15621">
            <title>Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2.</title>
            <authorList>
                <person name="Umitsu M."/>
                <person name="Nishimasu H."/>
                <person name="Noma A."/>
                <person name="Suzuki T."/>
                <person name="Ishitani R."/>
                <person name="Nureki O."/>
            </authorList>
            <dbReference type="PubMed" id="19717466"/>
            <dbReference type="DOI" id="10.1073/pnas.0905270106"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">S-adenosyl-L-methionine-dependent transferase that acts as a component of the wyosine derivatives biosynthesis pathway. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to 4-demethylwyosine (imG-14), forming 7-aminocarboxypropyl-demethylwyosine (wybutosine-86) at position 37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + H(+) + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.114" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64494">
        <property type="entry name" value="D64494"/>
    </dbReference>
    <dbReference type="PDB" id="3A27">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A=1-249"/>
    </dbReference>
    <dbReference type="PDBsum" id="3A27"/>
    <dbReference type="AlphaFoldDB" id="Q58952"/>
    <dbReference type="SMR" id="Q58952"/>
    <dbReference type="STRING" id="243232.MJ_1557"/>
    <dbReference type="EnsemblBacteria" id="AAB99577">
        <property type="protein sequence ID" value="AAB99577"/>
        <property type="gene ID" value="MJ_1557"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1557"/>
    <dbReference type="eggNOG" id="arCOG10124">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022610_0_2_2"/>
    <dbReference type="InParanoid" id="Q58952"/>
    <dbReference type="OMA" id="IHYHETV"/>
    <dbReference type="PhylomeDB" id="Q58952"/>
    <dbReference type="BRENDA" id="2.5.1.114">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58952"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01922">
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            <position position="80"/>
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        <ligand>
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        <ligand>
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    <feature type="binding site" evidence="1 2">
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    <feature type="binding site">
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        <ligand>
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        <location>
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        <location>
            <begin position="179"/>
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        <location>
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        <location>
            <begin position="200"/>
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        </location>
    </feature>
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        <location>
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    </feature>
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        <location>
            <begin position="206"/>
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        <location>
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        <location>
            <begin position="237"/>
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    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19717466"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="3A27"/>
        </source>
    </evidence>
    <sequence length="249" mass="29302" checksum="A4D1EB10B63CA51C" modified="1996-11-01" version="1">MGIKYQKIGDVVIVKKELSEDEIREIVKRTKCKAILLYTTQITGEFRTPHVKILYGKETETIHKEYGCLFKLDVAKIMWSQGNIEERKRMAFISNENEVVVDMFAGIGYFTIPLAKYSKPKLVYAIEKNPTAYHYLCENIKLNKLNNVIPILADNRDVELKDVADRVIMGYVHKTHKFLDKTFEFLKDRGVIHYHETVAEKIMYERPIERLKFYAEKNGYKLIDYEVRKIKKYAPGVWHVVVDAKFERI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58959</accession>
    <name>Y1564_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0201 protein MJ1564</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1564</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
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        <text evidence="2">Belongs to the UPF0201 family.</text>
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        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B/C/D=2-139"/>
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        </source>
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        <source>
            <dbReference type="PDB" id="2PZZ"/>
        </source>
    </evidence>
    <sequence length="170" mass="19330" checksum="F857091593FE494F" modified="1996-11-01" version="1">MEVIIKAKVKPTEDKYKVKKAILNIFPKAKLTFIEKDNEFGEWEGKTKSVEKLKELLRSQSILDAARMVLEKGMTENATKFYLNKQAAYVGAVNFDIDTHGGIFVKILADENEDIMKIIKDIAPRTKGGVIINEDELEEEEEKEDSEEIKEGHKEENNLKIKVIDNSSGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2020-12-02" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58966</accession>
    <name>Y1571_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ1571</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1571</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CbiQ family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="STRING" id="243232.MJ_1571"/>
    <dbReference type="EnsemblBacteria" id="AAB99588">
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        <property type="gene ID" value="MJ_1571"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1571"/>
    <dbReference type="eggNOG" id="arCOG02250">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056469_1_0_2"/>
    <dbReference type="InParanoid" id="Q58966"/>
    <dbReference type="OMA" id="MCYRYIF"/>
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    <dbReference type="Proteomes" id="UP000000805">
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    </dbReference>
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        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107419" description="Uncharacterized protein MJ1571">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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        </location>
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    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="292" mass="33670" checksum="28E02654E07163A7" modified="1996-11-01" version="1">MFKIYNIKSRIIRENMNNKLFDKTIEHVIKYLNENIFFEKYTRISGLLQNIESRIKIISLVIFLVGSVLSKHILTLIIFNSIALILAYLSNIPLLQYLKRVYVFIPIFAGIIAIPVMFNFMTPGKDVFVILNNPHISITYEGLIYAITFTLRVATCVSFAVLIPITTQWNKVTSAIHKLGVPEVVITITNLAYRYIFLLLNFVLDMMYSRKSRVVNKLGMVESWKEAGKAIGALFIKTYQMGEDXYYAMLSRGYNGEIKHIYREEIKIKDIAFLLFSIIITALLVLFDRGIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58980</accession>
    <name>DKFP_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Fructose-bisphosphate aldolase/6-deoxy-5-ketofructose 1-phosphate synthase</fullName>
            <ecNumber evidence="2">2.2.1.11</ecNumber>
            <ecNumber evidence="2">4.1.2.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>DKFP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Fructose-bisphosphate aldolase class 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Fructose-bisphosphate aldolase class I</fullName>
            <shortName>FBP aldolase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1585</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Biochemistry" volume="45" first="12366" last="12379">
            <title>Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="White R.H."/>
                <person name="Xu H."/>
            </authorList>
            <dbReference type="PubMed" id="17014089"/>
            <dbReference type="DOI" id="10.1021/bi061018a"/>
        </citation>
        <scope>FUNCTION AS A FRUCTOSE-BISPHOSPHATE ALDOLASE AND DKFP SYNTHASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the transaldolization of either fructose-1-P or fructose-1,6-bisphosphate with methylglyoxal to produce 6-deoxy-5-ketofructose-1-phosphate (DKFP). Also catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P or GAP) to produce fructose 1,6-bisphosphate (FBP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>beta-D-fructose 1,6-bisphosphate + methylglyoxal = 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + D-glyceraldehyde 3-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31911"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>beta-D-fructose 1-phosphate + methylglyoxal = 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + D-glyceraldehyde</text>
            <dbReference type="Rhea" id="RHEA:32259"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:17378"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:138881"/>
            <dbReference type="EC" id="2.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Aromatic compound metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DeoC/FbaB aldolase family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.11" evidence="2"/>
    <dbReference type="EC" id="4.1.2.13" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64497">
        <property type="entry name" value="H64497"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58980"/>
    <dbReference type="SMR" id="Q58980"/>
    <dbReference type="STRING" id="243232.MJ_1585"/>
    <dbReference type="EnsemblBacteria" id="AAB99604">
        <property type="protein sequence ID" value="AAB99604"/>
        <property type="gene ID" value="MJ_1585"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1585"/>
    <dbReference type="eggNOG" id="arCOG04044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057069_2_2_2"/>
    <dbReference type="InParanoid" id="Q58980"/>
    <dbReference type="OMA" id="FVKVNYP"/>
    <dbReference type="PhylomeDB" id="Q58980"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14590"/>
    <dbReference type="BRENDA" id="2.2.1.11">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000138958" description="Fructose-bisphosphate aldolase/6-deoxy-5-ketofructose 1-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with dihydroxyacetone-P" evidence="1">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="241"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="270"/>
            <end position="271"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17014089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="310" mass="34574" checksum="4AB6286F888049C0" modified="1996-11-01" version="1">MGIFMIKRLKKRDVKVPLTVPEDRKEEYIKNYLELTKRTGNVMLFAGDQKIEHLNDDFFGEGIAKDDASPEHLFNIASKGKICGFATQLGLIARYGMDYKKIPYIVKINSKTHLVKTRDPISRALVHVKDVVDLKENSGLKILGVGYTIYPGSEYEHIMFEEASRVILEAHKHGLIAIIWSYPRGKNVKDEKDPHLIAGAAGVAACLGADFVKVNYPKCDNPAERFKEAVLAAGRTGVLCAGGKSIEPEKFLKQIWEQINISGARGNATGRNIHQKPLDAAIRMCNAIYAITIEGKSLEEALKIYYGDRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-07-11" modified="2022-05-25" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>Q59042</accession>
    <name>PURQ_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="1">FGAM synthase</shortName>
            <ecNumber evidence="1">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="1">FGAR amidotransferase I</shortName>
            <shortName evidence="1">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Glutaminase PurQ</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purQ</name>
        <name type="ordered locus">MJ1648</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64505">
        <property type="entry name" value="F64505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871172.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59042"/>
    <dbReference type="SMR" id="Q59042"/>
    <dbReference type="STRING" id="243232.MJ_1648"/>
    <dbReference type="EnsemblBacteria" id="AAB99669">
        <property type="protein sequence ID" value="AAB99669"/>
        <property type="gene ID" value="MJ_1648"/>
    </dbReference>
    <dbReference type="GeneID" id="1452557"/>
    <dbReference type="KEGG" id="mja:MJ_1648"/>
    <dbReference type="eggNOG" id="arCOG00102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001031_3_1_2"/>
    <dbReference type="InParanoid" id="Q59042"/>
    <dbReference type="OMA" id="LHFVCRD"/>
    <dbReference type="OrthoDB" id="90817at2157"/>
    <dbReference type="PhylomeDB" id="Q59042"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100608" description="Phosphoribosylformylglycinamidine synthase subunit PurQ">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <sequence length="230" mass="25417" checksum="978792753DE169E1" modified="1996-11-01" version="1">MKIAVTKFLGTNCDLDVCHAVKLAGGEPELVFFTQENLDSYKGAVIPGGFSYGDYLRAGAISARTPIIKGLKKMVEEGKPVLGICNGAQIGLEAGFSKGTLTNNLNAKFICKWVYIRVENNKTPFTQYYKKGEVLKIPIAHAEGRFYADDETLDYMYKNNMIVFKYCDETGEVTEEANPNGSIDNIAGVCNENQNCVLLMPHPERASEKILGSDDGLKMFKGMIDYAKRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q59044</accession>
    <name>Y1650_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1650</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1650</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64505">
        <property type="entry name" value="H64505"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59044"/>
    <dbReference type="SMR" id="Q59044"/>
    <dbReference type="STRING" id="243232.MJ_1650"/>
    <dbReference type="EnsemblBacteria" id="AAB99671">
        <property type="protein sequence ID" value="AAB99671"/>
        <property type="gene ID" value="MJ_1650"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1650"/>
    <dbReference type="eggNOG" id="arCOG04149">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065087_0_0_2"/>
    <dbReference type="InParanoid" id="Q59044"/>
    <dbReference type="OMA" id="CHMCGSY"/>
    <dbReference type="PhylomeDB" id="Q59044"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="PTHR12746"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107458" description="Uncharacterized protein MJ1650">
        <location>
            <begin position="1"/>
            <end position="384"/>
        </location>
    </feature>
    <sequence length="384" mass="44427" checksum="75400E0724358C3F" modified="1996-11-01" version="1">MIMVNLRIINFIGDIMKIRGICYRCGAEDELIDGLCPICYAQEHPLIEVPDRVEIEVCHMCGSYKRKIWQTPKSEEAFEILNEIAYYATKDAIKKKSVMVEVEIYPEVTQLPGGKRSKLIIPVHIVARGRLPGEKEDRTYEKDIEVHLRMVQCPRCSRFMSNYYEATLQVRAMNRYLTEEEREELDNFVREELAKRLKKDRMAFIAKFIPQKEGLDYQLGSVGAARNVAQRIKEKYGGKITETATLVGVDRDSGKELYRVTVSVRVPEYKVGDVVEYKDKYYLVTAITEDKVYMKSIDYKREKIGLAWHIAEKETKMAKKKDELDTATVIATTPTIMVMDDKSYEVYEFDNIGDVKIKEGDKVKIFKKEGVSYLVNKIEGKDKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q59053</accession>
    <name>Y1659_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ1659</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1659</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64507">
        <property type="entry name" value="A64507"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59053"/>
    <dbReference type="SMR" id="Q59053"/>
    <dbReference type="STRING" id="243232.MJ_1659"/>
    <dbReference type="EnsemblBacteria" id="AAB99686">
        <property type="protein sequence ID" value="AAB99686"/>
        <property type="gene ID" value="MJ_1659"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1659"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q59053"/>
    <dbReference type="OMA" id="RCKYYLV"/>
    <dbReference type="PhylomeDB" id="Q59053"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184677" description="Uncharacterized ATP-binding protein MJ1659">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="361" mass="43147" checksum="AE223418058A8286" modified="1997-11-01" version="1">MVDSMEFFDREKEINYLVKVLSFEPNLIYFIYGPINSGKTTLIKHIIENKLDRDKYVVFYINLREHFISKYEDFIEVLFNTYEETFIEKLKKYLLSFINDLPKNIDVKSTILTGIPVPKNTLNEFLSTKNSENVFEYLTKIFEDIKKKGKQPILIIDELQKIGDLKINGFLIYELFNFFIDLTKEKHLCHVLCLSSDSLFIERVYNEGTLKDRCKYYLVDDFDYKTTKAFLKKHNFNDEEIDIVWHYFGGKPIKLVEAIQEKLLGEDVKEFCKKSLRVRKRQLKDLLYSLEDDNKELFERVIKLFENFKNRDEIFYEKISEEIVWTVKKNILFVNIEEGILKPQSKLDLLAIREILDEIKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q59055</accession>
    <name>PDXT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="1">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxT</name>
        <name type="ordered locus">MJ1661</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2007-04" db="PDB data bank">
            <title>Crystal structure of uncharacterized conserved protein from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Manzoku M."/>
                <person name="Ebihara A."/>
                <person name="Yokoyama S."/>
                <person name="Kuramitsu S."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H2O + L-glutamine = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EC" id="3.5.1.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64507">
        <property type="entry name" value="C64507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871185.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2YWJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-186"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YWJ"/>
    <dbReference type="AlphaFoldDB" id="Q59055"/>
    <dbReference type="SMR" id="Q59055"/>
    <dbReference type="STRING" id="243232.MJ_1661"/>
    <dbReference type="PRIDE" id="Q59055"/>
    <dbReference type="EnsemblBacteria" id="AAB99679">
        <property type="protein sequence ID" value="AAB99679"/>
        <property type="gene ID" value="MJ_1661"/>
    </dbReference>
    <dbReference type="GeneID" id="1452570"/>
    <dbReference type="KEGG" id="mja:MJ_1661"/>
    <dbReference type="eggNOG" id="arCOG00034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069674_2_0_2"/>
    <dbReference type="InParanoid" id="Q59055"/>
    <dbReference type="OMA" id="VFIRAPI"/>
    <dbReference type="OrthoDB" id="78046at2157"/>
    <dbReference type="PhylomeDB" id="Q59055"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="EvolutionaryTrace" id="Q59055"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PTHR31559"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135680" description="Pyridoxal 5'-phosphate synthase subunit PdxT">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="2"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="8"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="13"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="24"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="39"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="60"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="71"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="76"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="95"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="106"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="111"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="121"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="132"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="142"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="150"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="159"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="166"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="172"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2YWJ"/>
        </source>
    </evidence>
    <sequence length="186" mass="20597" checksum="FB1EB98EF5E982AA" modified="1997-11-01" version="1">MIIGVLAIQGDVEEHEEAIKKAGYEAKKVKRVEDLEGIDALIIPGGESTAIGKLMKKYGLLEKIKNSNLPILGTCAGMVLLSKGTGINQILLELMDITVKRNAYGRQVDSFEKEIEFKDLGKVYGVFIRAPVVDKILSDDVEVIARDGDKIVGVKQGKYMALSFHPELSEDGYKVYKYFVENCVKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q59066</accession>
    <name>CAS10_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)</fullName>
            <shortName>ssDNase Cas10</shortName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cyclic oligoadenylate synthase</fullName>
            <ecNumber evidence="1">2.7.7.-</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cas10</name>
        <name evidence="6" type="synonym">csm1</name>
        <name type="ordered locus">MJ1672</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2015" name="J. Mol. Biol." volume="427" first="259" last="273">
            <title>Crystal structure of the Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex.</title>
            <authorList>
                <person name="Numata T."/>
                <person name="Inanaga H."/>
                <person name="Sato C."/>
                <person name="Osawa T."/>
            </authorList>
            <dbReference type="PubMed" id="25451598"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2014.09.029"/>
        </citation>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities.</text>
    </comment>
    <comment type="function">
        <text evidence="2">This subunit is a single-strand-specific deoxyribonuclease (ssDNase) which digests both linear and circular ssDNA; it has both exo- and endonuclease activity.</text>
    </comment>
    <comment type="function">
        <text evidence="1">ssDNase activity is stimulated in the ternary Csm effector complex; binding of cognate target RNA activates the ssDNase, as the target RNA is degraded ssDNA activity decreases.</text>
    </comment>
    <comment type="function">
        <text evidence="1">When associated with the ternary Csm effector complex (the crRNA, Cas proteins and a cognate target ssRNA) synthesizes cyclic oligoadenylates (cOA) from ATP. cOAs are second messengers that stimulate the ssRNase activity of Csx1, inducing an antiviral state important for defense against invading nucleic acids.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1 5">Part of the Csm effector complex, that includes Cas10/Csm1, Csm2, Csm3, Csm4, Csm5 and mature crRNA (By similarity). Stable Cas10/Csm1-Csm4 and Cas10-Csm1-Csm3-Csm4 subcomplexes can be isolated; Cas10 and Csm3 probably do not directly interact (PubMed:25451598).</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The N-terminal HD domain has ssDNase activity. The C-terminal GGDEF domain has the cOA synthesis activity.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="7">Encoded in a type III-A CRISPR locus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CRISPR-associated Cas10/Csm1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EC" id="2.7.7.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64508">
        <property type="entry name" value="F64508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871196.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59066"/>
    <dbReference type="SMR" id="Q59066"/>
    <dbReference type="STRING" id="243232.MJ_1672"/>
    <dbReference type="EnsemblBacteria" id="AAB99690">
        <property type="protein sequence ID" value="AAB99690"/>
        <property type="gene ID" value="MJ_1672"/>
    </dbReference>
    <dbReference type="GeneID" id="1452581"/>
    <dbReference type="KEGG" id="mja:MJ_1672"/>
    <dbReference type="eggNOG" id="arCOG02666">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017487_0_0_2"/>
    <dbReference type="InParanoid" id="Q59066"/>
    <dbReference type="OMA" id="PSAFYYS"/>
    <dbReference type="PhylomeDB" id="Q59066"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09680">
        <property type="entry name" value="Cas10_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013408">
        <property type="entry name" value="Cas10/Csm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041062">
        <property type="entry name" value="Csm1_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18211">
        <property type="entry name" value="Csm1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02578">
        <property type="entry name" value="cas_TM1811_Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107470" description="CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)">
        <location>
            <begin position="1"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="GGDEF" evidence="3">
        <location>
            <begin position="578"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0A7HFE1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="B6YWB8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="25451598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="25451598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <sequence length="800" mass="93633" checksum="E4CD16D129C0FB0C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q60289</accession>
    <name>Y3532_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL32</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64513">
        <property type="entry name" value="G64513"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890079.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60289"/>
    <dbReference type="PRIDE" id="Q60289"/>
    <dbReference type="EnsemblBacteria" id="AAC37101">
        <property type="protein sequence ID" value="AAC37101"/>
        <property type="gene ID" value="MJ_ECL32"/>
    </dbReference>
    <dbReference type="GeneID" id="1450814"/>
    <dbReference type="KEGG" id="mja:MJ_ECL32"/>
    <dbReference type="eggNOG" id="arCOG06585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_644987_0_0_2"/>
    <dbReference type="InParanoid" id="Q60289"/>
    <dbReference type="OMA" id="IFECKSK"/>
    <dbReference type="OrthoDB" id="104965at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107516" description="Uncharacterized protein MJECL32">
        <location>
            <begin position="1"/>
            <end position="438"/>
        </location>
    </feature>
    <sequence length="438" mass="50925" checksum="DC8FB11505F5DEE3" modified="1997-11-01" version="1">MGKELDNRTLDFYFEKYGKKKTSTEPSYKDIIEYITALISENLEDYQSQHTKLINIILNLVYPIKYFPHHLYDLGYRPKKGSTLGVRIVLDGIYYTKNGNPAEITPDVILVNDNDTHVLIFECKSKSIKKEQLEKYLKLKENLHVIINKGYISVTKNPRLYKSDVSYMSFDDLTTHPVLKDTEDIQILKVSPGEISLERGNYENQKLNKIFPITWGEKEKPSYDLLICDAENDKEGSLFKILILRELVSMALSGHDEPEVGMVFSKKIILPKGEYSLESLKEDDFKEIENIIQKLCKKKCIIDKENKLLRVDKDIVILPENKPLNANVLKLIDALNISINMDSLVEKEFTVGTIMDALYRSTSISIIDYFYKDYKNIVSKRIEETLKLFSNRNLKGYLKKVSRKWRIKVIKDKRMARKFKESCKEEIGAIKSWQTTLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q60312</accession>
    <name>Y002_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0002</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0002</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64300">
        <property type="entry name" value="B64300"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869495.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60312"/>
    <dbReference type="STRING" id="243232.MJ_0002"/>
    <dbReference type="EnsemblBacteria" id="AAB97989">
        <property type="protein sequence ID" value="AAB97989"/>
        <property type="gene ID" value="MJ_0002"/>
    </dbReference>
    <dbReference type="GeneID" id="1450841"/>
    <dbReference type="KEGG" id="mja:MJ_0002"/>
    <dbReference type="eggNOG" id="arCOG12713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095303_0_0_2"/>
    <dbReference type="InParanoid" id="Q60312"/>
    <dbReference type="OMA" id="AHLYGFP"/>
    <dbReference type="OrthoDB" id="58968at2157"/>
    <dbReference type="PhylomeDB" id="Q60312"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018775">
        <property type="entry name" value="RlaP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34817">
        <property type="entry name" value="PTHR34817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10127">
        <property type="entry name" value="RlaP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106645" description="Uncharacterized protein MJ0002">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <sequence length="243" mass="28492" checksum="2B1BE2C59F816CB9" modified="1997-11-01" version="1">MEIFMEVPIFVVISGSDLYGIPNPSDVDIRGAHILDRELFIKNCLYKSKEEEVINKMFGKCDFVSFELGKFLRELLKPNANFIEIALSDKVLYSSKYHEDVKGIAYNCICKKLYHHWKGFAKPLQKLCEKESYNNPKTLLYILRAYYQGILCLESGEFKSDFSSFRCLDCYDEDIVSYLFECKVNKKPVDESYKKKIKSYFYELGVLLDESYKNSNLIDEPSETAKIKAIELYKKLYFEDVRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q60319</accession>
    <name>Y008_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0008</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0008</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64300">
        <property type="entry name" value="H64300"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60319"/>
    <dbReference type="SMR" id="Q60319"/>
    <dbReference type="STRING" id="243232.MJ_0008"/>
    <dbReference type="PRIDE" id="Q60319"/>
    <dbReference type="EnsemblBacteria" id="AAB97997">
        <property type="protein sequence ID" value="AAB97997"/>
        <property type="gene ID" value="MJ_0008"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0008"/>
    <dbReference type="eggNOG" id="arCOG01899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264580_0_0_2"/>
    <dbReference type="OMA" id="TIHLHNG"/>
    <dbReference type="PhylomeDB" id="Q60319"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106649" description="Uncharacterized protein MJ0008">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <sequence length="220" mass="25836" checksum="955DF4A0FAB71B88" modified="1997-11-01" version="1">MFCGSMIAICMRSKEGFLFNNKLMDWGLHYNPKIVKDNNIIGYHAPILDLDKKESIIILKNIIENIKGRDYLTIHLHNGKYGKINKETLIENLSIVNEFAEKNGIKLCIENLRKGFSSNPNNIIEIADEINCYITFDVGHIPYNRRLEFLEICSDRVYNSHVYEIEVDGKHLPPKNLNNLKPILDRLLDIKCKMFLIELMDIKEVLRTERMLKDYLEMYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q60358</accession>
    <name>MFNA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L-tyrosine/L-aspartate decarboxylase</fullName>
            <shortName evidence="1 6">TDC/ADC</shortName>
            <ecNumber evidence="1 3">4.1.1.11</ecNumber>
            <ecNumber evidence="1 2">4.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">mfnA</name>
        <name type="ordered locus">MJ0050</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochim. Biophys. Acta" volume="1722" first="175" last="182">
            <title>Identification and characterization of a L-tyrosine decarboxylase in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Kezmarsky N.D."/>
                <person name="Xu H."/>
                <person name="Graham D.E."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="15715981"/>
            <dbReference type="DOI" id="10.1016/j.bbagen.2004.12.003"/>
        </citation>
        <scope>FUNCTION AS A TYROSINE DECARBOXYLASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2014" name="J. Bacteriol." volume="196" first="2869" last="2875">
            <title>Beta-alanine biosynthesis in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Wang Y."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="24891443"/>
            <dbReference type="DOI" id="10.1128/JB.01784-14"/>
        </citation>
        <scope>FUNCTION AS AN ASPARTATE DECARBOXYLASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>PATHWAY</scope>
    </reference>
    <reference evidence="7" key="4">
        <citation type="submission" date="2008-11" db="PDB data bank">
            <title>Crystal structure of L-tyrosine decarboxylase MfnA (EC 4.1.1.25) (NP_247014.1) from Methanococcus jannaschii at 2.11 A resolution.</title>
            <authorList>
                <consortium name="Joint Center for Structural Genomics (JCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.11 ANGSTROMS) IN COMPLEX WITH PYRIDOXAL PHOSPHATE</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the decarboxylation of L-tyrosine to produce tyramine for methanofuran biosynthesis (PubMed:15715981). Can also catalyze the decarboxylation of L-aspartate to produce beta-alanine for coenzyme A (CoA) biosynthesis (PubMed:24891443).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>H(+) + L-tyrosine = CO2 + tyramine</text>
            <dbReference type="Rhea" id="RHEA:14345"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
            <dbReference type="EC" id="4.1.1.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>H(+) + L-aspartate = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by hydroxylamine and O-methylhydroxylamine.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">1.6 mM for L-tyrosine</KM>
            <KM evidence="3">0.8 mM for L-aspartate</KM>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 7.5-8.5 for tyrosine decarboxylase activity.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Thermostable. Retains full tyrosine decarboxylase activity after heating at 100 degrees Celsius for 10 minutes and 42% of its activity after 10 minutes at 110 degrees Celsius. Inactive after 10 minutes at 121 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1 2">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the group II decarboxylase family. MfnA subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="1 3"/>
    <dbReference type="EC" id="4.1.1.25" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64306">
        <property type="entry name" value="B64306"/>
    </dbReference>
    <dbReference type="PDB" id="3F9T">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.11 A"/>
        <property type="chains" value="A/B=1-396"/>
    </dbReference>
    <dbReference type="PDB" id="6JY1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.72 A"/>
        <property type="chains" value="A=1-396"/>
    </dbReference>
    <dbReference type="PDB" id="6LDR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.79 A"/>
        <property type="chains" value="A=1-396"/>
    </dbReference>
    <dbReference type="PDB" id="6LDS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A=1-396"/>
    </dbReference>
    <dbReference type="PDB" id="6LDT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.93 A"/>
        <property type="chains" value="A=1-396"/>
    </dbReference>
    <dbReference type="PDB" id="6M4Y">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-396"/>
    </dbReference>
    <dbReference type="PDBsum" id="3F9T"/>
    <dbReference type="PDBsum" id="6JY1"/>
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    <dbReference type="PDBsum" id="6LDT"/>
    <dbReference type="PDBsum" id="6M4Y"/>
    <dbReference type="AlphaFoldDB" id="Q60358"/>
    <dbReference type="SMR" id="Q60358"/>
    <dbReference type="STRING" id="243232.MJ_0050"/>
    <dbReference type="EnsemblBacteria" id="AAB98031">
        <property type="protein sequence ID" value="AAB98031"/>
        <property type="gene ID" value="MJ_0050"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0050"/>
    <dbReference type="eggNOG" id="arCOG00027">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028929_2_1_2"/>
    <dbReference type="InParanoid" id="Q60358"/>
    <dbReference type="OMA" id="DPHKMGL"/>
    <dbReference type="PhylomeDB" id="Q60358"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-12228"/>
    <dbReference type="BRENDA" id="4.1.1.11">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="4.1.1.25">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="EvolutionaryTrace" id="Q60358"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004837">
        <property type="term" value="F:tyrosine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0000304"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01610">
        <property type="entry name" value="MfnA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020931">
        <property type="entry name" value="MfnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03812">
        <property type="entry name" value="tyr_de_CO2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147023" description="L-tyrosine/L-aspartate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1 4">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
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            <end position="30"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
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            <end position="52"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="64"/>
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        </location>
    </feature>
    <feature type="strand" evidence="9">
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        </location>
    </feature>
    <feature type="helix" evidence="9">
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        </location>
    </feature>
    <feature type="strand" evidence="9">
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        </location>
    </feature>
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    <feature type="strand" evidence="9">
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    <feature type="strand" evidence="9">
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        </location>
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        </location>
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        </location>
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    <feature type="strand" evidence="9">
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        </location>
    </feature>
    <feature type="helix" evidence="9">
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    <feature type="helix" evidence="9">
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    <feature type="strand" evidence="8">
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15715981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="24891443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source ref="4"/>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="15715981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="3F9T"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="3F9T"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="6JY1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="6LDR"/>
        </source>
    </evidence>
    <sequence length="396" mass="45050" checksum="41CB8DEE45A8BBC0" modified="1997-11-01" version="1">MRNMQEKGVSEKEILEELKKYRSLDLKYEDGNIFGSMCSNVLPITRKIVDIFLETNLGDPGLFKGTKLLEEKAVALLGSLLNNKDAYGHIVSGGTEANLMALRCIKNIWREKRRKGLSKNEHPKIIVPITAHFSFEKGREMMDLEYIYAPIKEDYTIDEKFVKDAVEDYDVDGIIGIAGTTELGTIDNIEELSKIAKENNIYIHVDAAFGGLVIPFLDDKYKKKGVNYKFDFSLGVDSITIDPHKMGHCPIPSGGILFKDIGYKRYLDVDAPYLTETRQATILGTRVGFGGACTYAVLRYLGREGQRKIVNECMENTLYLYKKLKENNFKPVIEPILNIVAIEDEDYKEVCKKLRDRGIYVSVCNCVKALRIVVMPHIKREHIDNFIEILNSIKRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q60365</accession>
    <name>RIFK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Riboflavin kinase</fullName>
            <shortName>RFK</shortName>
            <ecNumber>2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ribK</name>
        <name type="ordered locus">MJ0056</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="J. Bacteriol." volume="190" first="2615" last="2618">
            <title>Identification and characterization of an archaeon-specific riboflavin kinase.</title>
            <authorList>
                <person name="Mashhadi Z."/>
                <person name="Zhang H."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="18245297"/>
            <dbReference type="DOI" id="10.1128/JB.01900-07"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>GENE NAME</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="submission" date="2007-03" db="PDB data bank">
            <title>Crystal structure of hypothetical protein from Methanococcus jannaschii bound to CDP.</title>
            <authorList>
                <consortium name="New York structural genomics research consortium (NYSGRC)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH CDP</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Structure" volume="15" first="1577" last="1590">
            <title>A CTP-dependent archaeal riboflavin kinase forms a bridge in the evolution of cradle-loop barrels.</title>
            <authorList>
                <person name="Ammelburg M."/>
                <person name="Hartmann M.D."/>
                <person name="Djuranovic S."/>
                <person name="Alva V."/>
                <person name="Koretke K.K."/>
                <person name="Martin J."/>
                <person name="Sauer G."/>
                <person name="Truffault V."/>
                <person name="Zeth K."/>
                <person name="Lupas A.N."/>
                <person name="Coles M."/>
            </authorList>
            <dbReference type="PubMed" id="18073108"/>
            <dbReference type="DOI" id="10.1016/j.str.2007.09.027"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF APOENZYME AND IN COMPLEX WITH REACTION PRODUCTS AND MAGNESIUM</scope>
        <scope>STRUCTURE BY NMR</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>CHARACTERIZATION</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN). Can also utilize UTP as the phosphate donor, although less efficiently, and it is unclear if ATP and GTP can also serve as substrates (PubMed:18245297) or not (PubMed:18073108).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>CTP + riboflavin = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text>Binds 1 magnesium ion per subunit. This ion is coordinated by a threonine and an asparagine, and by the alpha- and beta-phosphates of CDP.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">159 uM for riboflavin</KM>
            <KM evidence="2">1.8 mM for CTP</KM>
            <Vmax evidence="2">1.3 umol/min/mg enzyme</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3">Monomer.</text>
    </comment>
    <comment type="mass spectrometry" mass="15218.1" error="10.0" method="Electrospray" evidence="2"/>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98036" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98036.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64306">
        <property type="entry name" value="H64306"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496379.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2OYN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="2P3M">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="2VBS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="2VBT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="2VBU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=1-132"/>
    </dbReference>
    <dbReference type="PDB" id="2VBV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-132"/>
    </dbReference>
    <dbReference type="PDBsum" id="2OYN"/>
    <dbReference type="PDBsum" id="2P3M"/>
    <dbReference type="PDBsum" id="2VBS"/>
    <dbReference type="PDBsum" id="2VBT"/>
    <dbReference type="PDBsum" id="2VBU"/>
    <dbReference type="PDBsum" id="2VBV"/>
    <dbReference type="AlphaFoldDB" id="Q60365"/>
    <dbReference type="BMRB" id="Q60365"/>
    <dbReference type="SMR" id="Q60365"/>
    <dbReference type="STRING" id="243232.MJ_0056"/>
    <dbReference type="EnsemblBacteria" id="AAB98036">
        <property type="protein sequence ID" value="AAB98036"/>
        <property type="gene ID" value="MJ_0056"/>
    </dbReference>
    <dbReference type="GeneID" id="1450895"/>
    <dbReference type="KEGG" id="mja:MJ_0056"/>
    <dbReference type="eggNOG" id="arCOG01904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140165_0_0_2"/>
    <dbReference type="InParanoid" id="Q60365"/>
    <dbReference type="OrthoDB" id="91297at2157"/>
    <dbReference type="PhylomeDB" id="Q60365"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-13864"/>
    <dbReference type="BRENDA" id="2.7.1.161">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q60365"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q60365"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01285">
        <property type="entry name" value="Riboflavin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023470">
        <property type="entry name" value="Riboflavin_kinase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="PTHR40706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="SSF82114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106673" description="Riboflavin kinase">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="1"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="13"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="40"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="56"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="61"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="66"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="99"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="121"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="18073108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18245297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source ref="3"/>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2P3M"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2VBU"/>
        </source>
    </evidence>
    <sequence length="132" mass="15214" checksum="12B9AF1567F7D8B8" modified="2011-04-05" version="2">MIIEGEVVSGLGEGRYFLSLPPYKEIFKKILGFEPYEGTLNLKLDREFDINKFKYIETEDFEFNGKRFFGVKVLPIKILIGNKKIDGAIVVPKKTYHSSEIIEIIAPMKLREQFNLKDGDVIKILIKGDKDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>O06917</accession>
    <name>Y263_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TPR repeat-containing protein MJ0263</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0263</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Trends Biochem. Sci." volume="23" first="245" last="247">
            <title>Tetratrico-peptide-repeat proteins in the archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="Kyrpides N.C."/>
                <person name="Woese C.R."/>
            </authorList>
            <dbReference type="PubMed" id="9697413"/>
            <dbReference type="DOI" id="10.1016/S0968-0004(98)01228-6"/>
        </citation>
        <scope>DOMAINS TPR REPEATS</scope>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64332">
        <property type="entry name" value="H64332"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869760.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O06917"/>
    <dbReference type="SMR" id="O06917"/>
    <dbReference type="STRING" id="243232.MJ_0263"/>
    <dbReference type="EnsemblBacteria" id="AAB98249">
        <property type="protein sequence ID" value="AAB98249"/>
        <property type="gene ID" value="MJ_0263"/>
    </dbReference>
    <dbReference type="GeneID" id="1451117"/>
    <dbReference type="KEGG" id="mja:MJ_0263"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003728_2_5_2"/>
    <dbReference type="InParanoid" id="O06917"/>
    <dbReference type="OMA" id="CYLSPFH"/>
    <dbReference type="OrthoDB" id="66157at2157"/>
    <dbReference type="PhylomeDB" id="O06917"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019440">
        <property type="entry name" value="MAU2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10345">
        <property type="entry name" value="Cohesin_load"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0802">TPR repeat</keyword>
    <feature type="chain" id="PRO_0000106456" description="TPR repeat-containing protein MJ0263">
        <location>
            <begin position="1"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 1">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 2">
        <location>
            <begin position="46"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 3">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 4">
        <location>
            <begin position="114"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 5">
        <location>
            <begin position="148"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 6">
        <location>
            <begin position="182"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 7">
        <location>
            <begin position="216"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 8">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 9">
        <location>
            <begin position="289"/>
            <end position="320"/>
        </location>
    </feature>
    <sequence length="320" mass="36862" checksum="8D818C197D8B0352" modified="1997-07-01" version="1">MDYDNMVKTLEILKDVVNALECADKGNFDKALEYLEKAQKVDKDNPLVLYVKGIVLKLKGDMEKAEKYFECLENIEGTSLLSLGNLICLTFVKGEYERTLKYIEKLSRLSKPCYLSPFHKALIYIEFGEFEKALEALDEFLKIYPNLTSILRQKASILEILGKLDEALDCVNKILSIKKDDAHAWYLKGRILKKLGNIKEALDALKMAINLNENLVHVYKDIAYLELANNNYEEALNYITKYLEKFPNDVEAKFYLALIYENLNKVDDALKIYDKIISNKNVKDKLLIKSSILNKARILEKLGKIEEAVETYNKAFDNNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>P54020</accession>
    <name>RS4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps4</name>
        <name type="ordered locus">MJ0190</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64323">
        <property type="entry name" value="G64323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869685.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54020"/>
    <dbReference type="SMR" id="P54020"/>
    <dbReference type="STRING" id="243232.MJ_0190"/>
    <dbReference type="PRIDE" id="P54020"/>
    <dbReference type="EnsemblBacteria" id="AAB98170">
        <property type="protein sequence ID" value="AAB98170"/>
        <property type="gene ID" value="MJ_0190"/>
    </dbReference>
    <dbReference type="GeneID" id="1451038"/>
    <dbReference type="KEGG" id="mja:MJ_0190"/>
    <dbReference type="eggNOG" id="arCOG04239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089738_1_1_2"/>
    <dbReference type="InParanoid" id="P54020"/>
    <dbReference type="OMA" id="ARQFITH"/>
    <dbReference type="OrthoDB" id="93256at2157"/>
    <dbReference type="PhylomeDB" id="P54020"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_S4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_S4/S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_S4/S9_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_S4/S9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_S4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_S4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="PTHR11831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000132508" description="30S ribosomal protein S4">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="S4 RNA-binding" evidence="1">
        <location>
            <begin position="105"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <sequence length="187" mass="22050" checksum="7D4C51389D5C8A12" modified="1996-10-01" version="1">MGDPRRRFKKTYETPNHPWIKERIEREKELCRKYGLRRKREVWKAETILRKYRRQARRLISDRTEQGAKEAVQLFNVLKKYGILKIENPTLDDVLSLTVEDILERRLQTLVFRKGLARTPRQARQLIVHGHIAVNGRVVTAPSYMVTVEEEDKISYAKNSPFNDDNHPERAKIVGLVAEETQTQETE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>P54034</accession>
    <name>RS3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3</name>
        <name type="ordered locus">MJ0461</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64357">
        <property type="entry name" value="E64357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869961.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54034"/>
    <dbReference type="SMR" id="P54034"/>
    <dbReference type="STRING" id="243232.MJ_0461"/>
    <dbReference type="EnsemblBacteria" id="AAB98450">
        <property type="protein sequence ID" value="AAB98450"/>
        <property type="gene ID" value="MJ_0461"/>
    </dbReference>
    <dbReference type="GeneID" id="1451323"/>
    <dbReference type="KEGG" id="mja:MJ_0461"/>
    <dbReference type="eggNOG" id="arCOG04097">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058591_1_1_2"/>
    <dbReference type="InParanoid" id="P54034"/>
    <dbReference type="OMA" id="YIKKCGE"/>
    <dbReference type="OrthoDB" id="60366at2157"/>
    <dbReference type="PhylomeDB" id="P54034"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_S3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_S3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_S3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_S3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_S3_euk/arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="SSF54821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130250" description="30S ribosomal protein S3">
        <location>
            <begin position="1"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="KH type-2" evidence="1">
        <location>
            <begin position="16"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <sequence length="208" mass="23325" checksum="C78D8E96A5A22A6A" modified="1996-10-01" version="1">MIERTFVKENVKRLLIDEYFKKELSKAGYSHCDIRKTPIGTKIIIYAEKPGFVIGRRGSRIRELTETLAKEFGVEKPQIDVKPVENPDLDAQVVAQKVAQSLERGLHFRRVGHTAVRRVMNAGAKGVIVIISGKLTGERARTEKFMAGYMKHCGEPAEELVDKGRAIAKTKPGVIGVTVKIMRPDVLLPDEIIIKEDAEVKHVVEEEQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>P54065</accession>
    <name>RS28_METJA</name>
    <protein>
        <recommendedName>
            <fullName>30S ribosomal protein S28e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rps28e</name>
        <name type="ordered locus">MJ1202</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99206" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99206.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64450">
        <property type="entry name" value="A64450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012980426.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54065"/>
    <dbReference type="SMR" id="P54065"/>
    <dbReference type="STRING" id="243232.MJ_1202"/>
    <dbReference type="EnsemblBacteria" id="AAB99206">
        <property type="protein sequence ID" value="AAB99206"/>
        <property type="gene ID" value="MJ_1202"/>
    </dbReference>
    <dbReference type="GeneID" id="8804675"/>
    <dbReference type="KEGG" id="mja:MJ_1202"/>
    <dbReference type="eggNOG" id="arCOG04314">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_178987_2_1_2"/>
    <dbReference type="InParanoid" id="P54065"/>
    <dbReference type="OMA" id="MHGEASQ"/>
    <dbReference type="OrthoDB" id="121548at2157"/>
    <dbReference type="PhylomeDB" id="P54065"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_S28e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_S28e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="PTHR10769"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000136848" description="30S ribosomal protein S28e">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="75" mass="8364" checksum="9827B6CAB41B65E6" modified="2001-01-11" version="2">MEDEFVYKEAVAAEVIEVIGRTGVTGGIIQVRCKILGGKDTGRVLVRNVKGPVKVGDIIMLRETEREARPLDRRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-02-12" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>P59283</accession>
    <name>RPO12_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 3">DNA-directed RNA polymerase subunit P</fullName>
            <shortName evidence="3">mjP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo12</name>
        <name evidence="1" type="synonym">rpoP</name>
        <name type="ordered locus">MJ0593.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Nucleic Acids Res." volume="28" first="4299" last="4305">
            <title>Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12.</title>
            <authorList>
                <person name="Werner F."/>
                <person name="Eloranta J.J."/>
                <person name="Weinzierl R.O."/>
            </authorList>
            <dbReference type="PubMed" id="11058130"/>
            <dbReference type="DOI" id="10.1093/nar/28.21.4299"/>
        </citation>
        <scope>INTERACTION WITH RPO3</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the RNA polymerase complex (By similarity). Interacts with Rpo3. Forms an Rpo3-Rpo10-Rpo11-Rpo12 complex upon coexpression (PubMed:11058130).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496553.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P59283"/>
    <dbReference type="SMR" id="P59283"/>
    <dbReference type="GeneID" id="27929961"/>
    <dbReference type="InParanoid" id="P59283"/>
    <dbReference type="OrthoDB" id="129924at2157"/>
    <dbReference type="PhylomeDB" id="P59283"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="SSF63393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000159758" description="DNA-directed RNA polymerase subunit Rpo12">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <sequence length="46" mass="5452" checksum="6B66BC918364EFA0" modified="2003-02-12" version="1">MVEYKCLNCKKIIKLEELGKRARCPHCSYKILVKLRPKVVKHVKAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>P81313</accession>
    <name>Y79A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0792.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0792.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870300.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81313"/>
    <dbReference type="SMR" id="P81313"/>
    <dbReference type="STRING" id="243232.MJ_0792.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98800">
        <property type="protein sequence ID" value="AAB98800"/>
        <property type="gene ID" value="MJ_0792.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451672"/>
    <dbReference type="KEGG" id="mja:MJ_0792.1"/>
    <dbReference type="HOGENOM" id="CLU_1631661_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107043" description="Uncharacterized protein MJ0792.1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="162" mass="18866" checksum="4057E5A837BEB01C" modified="1998-07-15" version="1">MNFEKKLNGILSFTYLALVLCLVMPFMLILVLDTTFTFNKFNLNFVYRQIVELIVLSIFAGFITSFALYNKICRISTEELKRDILENNGLKTIFKIAQYEVMVSIFYSLLLLIILLNKQLLYYGFTAIFQIFFTFCAIFVPLFIFGSLVCRTILLHKIRGTT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>P81322</accession>
    <name>Y83A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0333 protein MJ0832.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0832.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0333 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870344.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81322"/>
    <dbReference type="STRING" id="243232.MJ_0832.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98840">
        <property type="protein sequence ID" value="AAB98840"/>
        <property type="gene ID" value="MJ_0832.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451716"/>
    <dbReference type="KEGG" id="mja:MJ_0832.1"/>
    <dbReference type="eggNOG" id="arCOG05053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912362_0_0_2"/>
    <dbReference type="OMA" id="VGYPGMF"/>
    <dbReference type="OrthoDB" id="116062at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000218266" description="UPF0333 protein MJ0832.1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="142" mass="15689" checksum="A76E07F498D74FC0" modified="1998-07-15" version="1">MLKFRKRGQISLEFSLLFLGVLLAIVIAVGYPGMFGFNKTVSISSMSLAHAAVSKMKQNIELVSSADEGTMKIVYIKCPPGTWGANNNILYFYRDGNIKFNITAKCDINIILNGNKTVSTPKIIIANITKIDETHVIVTLYQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>P81328</accession>
    <name>YE1A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1417.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1417.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81328"/>
    <dbReference type="SMR" id="P81328"/>
    <dbReference type="STRING" id="243232.MJ_1417.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99431">
        <property type="protein sequence ID" value="AAB99431"/>
        <property type="gene ID" value="MJ_1417.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1417.1"/>
    <dbReference type="HOGENOM" id="CLU_1782506_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107316" description="Uncharacterized protein MJ1417.1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="145" mass="17308" checksum="A37763D64BAD99C6" modified="1998-07-15" version="1">MYLIWIIGGGQKDLTNKILDEIKKLYEQLDEIKDKNINSSNVQLNEFMRQNVNMLKELNQKIDKYLENNNEILKEMEKYVKEDIEHKNRMERKLKQISMLLLIVIIAIGLTISYMVILNNEYLSTQLFNYIQIAIQYLKSLLSNY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57570</accession>
    <name>Y106_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0106</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0106</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64313">
        <property type="entry name" value="B64313"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57570"/>
    <dbReference type="SMR" id="Q57570"/>
    <dbReference type="STRING" id="243232.MJ_0106"/>
    <dbReference type="PRIDE" id="Q57570"/>
    <dbReference type="DNASU" id="1450947"/>
    <dbReference type="EnsemblBacteria" id="AAB98088">
        <property type="protein sequence ID" value="AAB98088"/>
        <property type="gene ID" value="MJ_0106"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0106"/>
    <dbReference type="eggNOG" id="arCOG00347">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_0_0_2"/>
    <dbReference type="InParanoid" id="Q57570"/>
    <dbReference type="OMA" id="MPVRIYE"/>
    <dbReference type="PhylomeDB" id="Q57570"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004425">
        <property type="entry name" value="CHP00061"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00161">
        <property type="entry name" value="TIGR00161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106695" description="Uncharacterized protein MJ0106">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="238" mass="26473" checksum="CF30383FF83551DB" modified="1996-11-01" version="1">MVDSMKFVEKAKIEFENPIVIEAFPGTGLVGSIAGFQIIKDLNLKYFGYFEVDGILPLTTIEKGIPYPPVRAYANKDFIILFSDIIISPFKINGLAEFIVKTFSNKNPKLFVSLGGIMAGKSEKVFGIANKEELIEDLKNYVEIFDFGVVGGMGGNLLIKCHDNGFDAIGLLAETVGIRPDPRGGANLLEVLNKMFNLNVNIENLIKEAEAIENKLKELAEQHLKMMSKSRKEYPMYI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57576</accession>
    <name>ACDG_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit gamma</fullName>
            <shortName evidence="1">ACDS complex subunit gamma</shortName>
            <ecNumber evidence="1">2.1.1.245</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-methyltetrahydrosarcinapterin:corrinoid/iron-sulfur protein Co-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">ACDS complex methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Corrinoid/iron-sulfur component large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhE</name>
        <name type="ordered locus">MJ0112</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5,6,7,8-tetrahydrosarcinapterin + methyl-Co(III)-[corrinoid Fe-S protein] = 5-methyltetrahydrosarcinapterin + Co(I)-[corrinoid Fe-S protein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45196"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59924"/>
            <dbReference type="ChEBI" id="CHEBI:64267"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.1.1.245"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>corrinoid</name>
            <dbReference type="ChEBI" id="CHEBI:33913"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <dbReference type="EC" id="2.1.1.245" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64313">
        <property type="entry name" value="H64313"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869604.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57576"/>
    <dbReference type="SMR" id="Q57576"/>
    <dbReference type="STRING" id="243232.MJ_0112"/>
    <dbReference type="EnsemblBacteria" id="AAB98093">
        <property type="protein sequence ID" value="AAB98093"/>
        <property type="gene ID" value="MJ_0112"/>
    </dbReference>
    <dbReference type="GeneID" id="1450953"/>
    <dbReference type="KEGG" id="mja:MJ_0112"/>
    <dbReference type="eggNOG" id="arCOG01979">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050002_0_0_2"/>
    <dbReference type="InParanoid" id="Q57576"/>
    <dbReference type="OMA" id="SCMAFAT"/>
    <dbReference type="OrthoDB" id="31154at2157"/>
    <dbReference type="PhylomeDB" id="Q57576"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046356">
        <property type="term" value="P:acetyl-CoA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01136">
        <property type="entry name" value="CdhE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016218">
        <property type="entry name" value="AcylCoA_decarb/synth_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023427">
        <property type="entry name" value="AcylCoA_decarb/synth_gsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000376">
        <property type="entry name" value="AcCoA_decarb_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155120" description="Acetyl-CoA decarbonylase/synthase complex subunit gamma">
        <location>
            <begin position="1"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S" evidence="2">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00989"/>
        </source>
    </evidence>
    <sequence length="488" mass="55027" checksum="AE09B3CECD238B5A" modified="1996-11-01" version="1">MPKKISAMDIYKLLPKTNCKKCGYPSCMAFATKLLEKEATIDQCPILNTPKFEKNKKKIIELISPPVKEVWFGNEEKKAVMGGDEVMYRYQLSFFNPTPIGVDISDELSEEEIKNRAKEIENFVFERTGEKLKLDFIVIRNASGDVEKFKKAIEIVEKETKMPICIASLNPEVIKEALKVVKSKPMVYAATKETLNDFIKVIKEVKKDVVLVLSSNNVKDLKNMAAKCLANGIEDLVLEPHTYPENIAETLDLNVMIRRSAIEKEDKYLGFPILNLPINAYYYALKNECPISGFFEDKEVVAKMFEATIANTLMNRYADALIMHGMDIWELMPVLTLRQCIYTDPRKPQAVEPGLYPIGNPDENSPVILTTNFSLTFYTVTGDFEKDNVTCWLLVMDTGGKAVDVSVAGGQYNGENAKKLIEETGIADKVSHRIIILPALAASTRGDIEDKTGWTCVVGTRDSSQVGDFLRNNWDKILKEWKEKNQTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57579</accession>
    <name>Y115_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0115</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0115</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64314">
        <property type="entry name" value="C64314"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869607.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57579"/>
    <dbReference type="SMR" id="Q57579"/>
    <dbReference type="STRING" id="243232.MJ_0115"/>
    <dbReference type="PRIDE" id="Q57579"/>
    <dbReference type="EnsemblBacteria" id="AAB98096">
        <property type="protein sequence ID" value="AAB98096"/>
        <property type="gene ID" value="MJ_0115"/>
    </dbReference>
    <dbReference type="GeneID" id="1450957"/>
    <dbReference type="KEGG" id="mja:MJ_0115"/>
    <dbReference type="eggNOG" id="arCOG04853">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1727260_0_0_2"/>
    <dbReference type="OMA" id="PCPFRDS"/>
    <dbReference type="OrthoDB" id="96489at2157"/>
    <dbReference type="PhylomeDB" id="Q57579"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017671">
        <property type="entry name" value="Methan_mark_9"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03277">
        <property type="entry name" value="methan_mark_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106699" description="Uncharacterized protein MJ0115">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <sequence length="155" mass="17727" checksum="E28BB0FD12CB1745" modified="1997-11-01" version="1">MGWENAPSHICRGGDLRGLAFCCPPIKYCPIHKALAVLKMSPEEFIRIKEEFGKRTKLGLGENTCFGSLVWCCKITKPCPYRDYELAKNNISPDEYMELKKQLAEEIIRNSQFFKEAVEVFVKKGIPKDIAEKCILETGDLKKAYEMAIKMIDKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q57583</accession>
    <name>Y119_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0119</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0119</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64314">
        <property type="entry name" value="G64314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57583"/>
    <dbReference type="SMR" id="Q57583"/>
    <dbReference type="STRING" id="243232.MJ_0119"/>
    <dbReference type="PRIDE" id="Q57583"/>
    <dbReference type="EnsemblBacteria" id="AAB98100">
        <property type="protein sequence ID" value="AAB98100"/>
        <property type="gene ID" value="MJ_0119"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0119"/>
    <dbReference type="eggNOG" id="arCOG00295">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087825_0_0_2"/>
    <dbReference type="OMA" id="CMMQKVH"/>
    <dbReference type="PhylomeDB" id="Q57583"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106701" description="Uncharacterized protein MJ0119">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S" evidence="1">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00989"/>
        </source>
    </evidence>
    <sequence length="224" mass="25680" checksum="572D7C9260859992" modified="1997-11-01" version="1">MDSWNMKGSKMVDVNEITKYLPGFNCGACGYKRCDLFAEALLNKDVKLEDCPFLLRERFKENYEKLKEILKIKGKIKKEEKYIGVIDGYEADFLLKPLPNECSCRETLLIMDKKELKVGDYIRYRPLGCPIPHFAKIIDEYHGFYIIHVVGPSHRITGEKIEYKDVGIAIVVAFEGIVEGKVPEVGKTVKFIPKHCMMQKVHSGVVVQVEGKRVYIEGIDLKVF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57589</accession>
    <name>Y125_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative RNase MJ0125</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative toxin MJ0125</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0125</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable toxic component of a putative type VII toxin-antitoxin (TA) system, probably an RNase. Probably neutralized by cognate antitoxin MJ0126. Neutralization may be due to AMPylation by MJ0126.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer, probably forms a complex with cognate antitoxin MJ0126.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Modified by cognate antitoxin MJ0126; probably at least 2 successive AMPylation events occur on Tyr-83.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64315">
        <property type="entry name" value="E64315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869618.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57589"/>
    <dbReference type="SMR" id="Q57589"/>
    <dbReference type="STRING" id="243232.MJ_0125"/>
    <dbReference type="EnsemblBacteria" id="AAB98105">
        <property type="protein sequence ID" value="AAB98105"/>
        <property type="gene ID" value="MJ_0125"/>
    </dbReference>
    <dbReference type="GeneID" id="1450967"/>
    <dbReference type="KEGG" id="mja:MJ_0125"/>
    <dbReference type="eggNOG" id="arCOG05024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142825_3_3_2"/>
    <dbReference type="InParanoid" id="Q57589"/>
    <dbReference type="OMA" id="EQFTTGI"/>
    <dbReference type="OrthoDB" id="112390at2157"/>
    <dbReference type="PhylomeDB" id="Q57589"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000158259" description="Putative RNase MJ0125">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RX(4)HXY motif" evidence="1">
        <location>
            <begin position="76"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-di-AMP-tyrosine" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJR1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="116" mass="13970" checksum="7A77740A495160EF" modified="1997-11-01" version="1">MPKRDIKAFLYDILSYMDDIINFTKDMDYEEFINNKAIKYAVIRCLEVIGEAVKKIPKDIREKYPHIPFKELAGMRDKLIHQYFGVDYLTVWETAKYEIPEIKKEFEKIIKDLEEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q57604</accession>
    <name>MJK1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Potassium channel protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>MjK1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0138.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="FEBS Lett." volume="547" first="165" last="169">
            <title>MjK1, a K+ channel from M. jannaschii, mediates K+ uptake and K+ sensitivity in E. coli.</title>
            <authorList>
                <person name="Hellmer J."/>
                <person name="Zeilinger C."/>
            </authorList>
            <dbReference type="PubMed" id="12860407"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(03)00706-3"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Potassium channel protein. Seems to conduct potassium at low membrane potentials.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Inhibited by barium and cesium.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64317">
        <property type="entry name" value="D64317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869633.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57604"/>
    <dbReference type="SMR" id="Q57604"/>
    <dbReference type="STRING" id="243232.MJ_0138.1"/>
    <dbReference type="TCDB" id="1.A.1.13.8">
        <property type="family name" value="the voltage-gated ion channel (vic) superfamily"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q57604"/>
    <dbReference type="EnsemblBacteria" id="AAB98130">
        <property type="protein sequence ID" value="AAB98130"/>
        <property type="gene ID" value="MJ_0138.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1450981"/>
    <dbReference type="KEGG" id="mja:MJ_0138.1"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050982_0_1_2"/>
    <dbReference type="InParanoid" id="Q57604"/>
    <dbReference type="OMA" id="VFEGIWW"/>
    <dbReference type="OrthoDB" id="80000at2157"/>
    <dbReference type="PhylomeDB" id="Q57604"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0631">Potassium channel</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000054099" description="Potassium channel protein 1">
        <location>
            <begin position="1"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="28"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="82"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="4">
        <location>
            <begin position="246"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Selectivity filter" evidence="1">
        <location>
            <begin position="46"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="12860407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="333" mass="37150" checksum="7CC082675F96B06A" modified="1997-11-01" version="1">METYEKIELGIIVIILLILIESVILMTVEGWDFFTAFYTAVVTISTVGYGDYTPQTFLGKLSVIIYIFAGVGAVAYTMGNIASFFIEGHFRKYFRLRKMMDRIKKLNNHYIICGYGRLGKVIAEEFKKCNIPFVIIDSDEKLLEEALEKDPNLICIVGDATSDDILKKAKIEKAKGLISVVSSDAENVFITLSAKKLNPNIYIVAKAEKPSTLDKLIKAGADRAVCPYIVGGMEIARIAINPDIVEFIHSLVATEEDMEVRRYIVKNKELDNKLLKDSGIREKTGATILAVKKGDKTITSPPPDTVINIGDIIYAFGTKEQLEKLKRYVEGVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q57643</accession>
    <name>Y184_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0184</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0184</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64323">
        <property type="entry name" value="A64323"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57643"/>
    <dbReference type="SMR" id="Q57643"/>
    <dbReference type="STRING" id="243232.MJ_0184"/>
    <dbReference type="EnsemblBacteria" id="AAB98181">
        <property type="protein sequence ID" value="AAB98181"/>
        <property type="gene ID" value="MJ_0184"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0184"/>
    <dbReference type="HOGENOM" id="CLU_2629740_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106733" description="Uncharacterized protein MJ0184">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="77" mass="9150" checksum="A5164FA166378C68" modified="1996-11-01" version="1">MNIMITKQFDRHLKYYTTIVKVFANGIILITAYYLVFELPVGYLIGLYIIMFVVWLLVSMFFLGRLLDFMAKMDLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q57697</accession>
    <name>PCNA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="1">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pcn</name>
        <name type="ordered locus">MJ0247</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PCNA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64330">
        <property type="entry name" value="H64330"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57697"/>
    <dbReference type="SMR" id="Q57697"/>
    <dbReference type="STRING" id="243232.MJ_0247"/>
    <dbReference type="EnsemblBacteria" id="AAB98235">
        <property type="protein sequence ID" value="AAB98235"/>
        <property type="gene ID" value="MJ_0247"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0247"/>
    <dbReference type="eggNOG" id="arCOG00488">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043978_1_0_2"/>
    <dbReference type="InParanoid" id="Q57697"/>
    <dbReference type="OMA" id="EMKLINM"/>
    <dbReference type="PhylomeDB" id="Q57697"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PTHR11352:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149196" description="DNA polymerase sliding clamp">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <sequence length="247" mass="27638" checksum="28E8A363741931A5" modified="1996-11-01" version="1">MFRGVMESAKEFKKVVDTISTLLDEICFEVDEEGIKASAMDPSHVALVSLEIPRLAFEEYEADSHDIGIDLEAFKKVMNRAKAKDRLILELDEEKNKLNVIFENTGKRKFSLALLDISASSVKVPEIEYPNVIMIKGDAFKEALKDADLFSDYVILKADEDKFVIHAKGDLNENEAIFEKDSSAIISLEVKEEAKSAFNLDYLMDMVKGVSSGDIIKIYLGNDMPLKLEYSIAGVNLTFLLAPRIEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q57719</accession>
    <name>Y271_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0271</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0271</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64333">
        <property type="entry name" value="H64333"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869768.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57719"/>
    <dbReference type="DNASU" id="1451125"/>
    <dbReference type="EnsemblBacteria" id="AAB98260">
        <property type="protein sequence ID" value="AAB98260"/>
        <property type="gene ID" value="MJ_0271"/>
    </dbReference>
    <dbReference type="GeneID" id="1451125"/>
    <dbReference type="KEGG" id="mja:MJ_0271"/>
    <dbReference type="eggNOG" id="arCOG12716">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1493004_0_0_2"/>
    <dbReference type="OMA" id="ANMFIIE"/>
    <dbReference type="OrthoDB" id="111937at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106764" description="Uncharacterized protein MJ0271">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="180" mass="20813" checksum="F306D3E4BC0ECFB6" modified="1996-11-01" version="1">MDLKSNIKLILATDLLAVLILSLFIKNFKMVLAFLLAVFVIWLFIDKNNINERLYENLLAMSVGFIEGILIFLGIIYNEVFLDITLGIFAILILIVMGILFPKYKLIFEVFDEFVEHLKQKSGFLTLISIFGMLLTIYVFLLILPSKEFCINAVDIIRTIMLVITANMFIIEFYTFKKFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57743</accession>
    <name>FDHD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein FdhD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fdhD</name>
        <name type="ordered locus">MJ0295</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for formate dehydrogenase (FDH) activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FdhD family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64336">
        <property type="entry name" value="H64336"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869793.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57743"/>
    <dbReference type="SMR" id="Q57743"/>
    <dbReference type="STRING" id="243232.MJ_0295"/>
    <dbReference type="EnsemblBacteria" id="AAB98280">
        <property type="protein sequence ID" value="AAB98280"/>
        <property type="gene ID" value="MJ_0295"/>
    </dbReference>
    <dbReference type="GeneID" id="1451150"/>
    <dbReference type="KEGG" id="mja:MJ_0295"/>
    <dbReference type="eggNOG" id="arCOG04358">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056887_4_2_2"/>
    <dbReference type="InParanoid" id="Q57743"/>
    <dbReference type="OMA" id="WAALFDF"/>
    <dbReference type="OrthoDB" id="68783at2157"/>
    <dbReference type="PhylomeDB" id="Q57743"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00187">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="PTHR30592"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015626">
        <property type="entry name" value="FdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="SSF53927"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00129">
        <property type="entry name" value="fdhD_narQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152936" description="Protein FdhD">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="210"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00187"/>
        </source>
    </evidence>
    <sequence length="227" mass="26119" checksum="80CCC2FCF90D43CC" modified="1996-11-01" version="1">MIKKVKIKKFNGRDFYDMEDYVAVEESYNIFINGEFVKSLSMSPNFLNEFAVGFAISEGFLNKIDKVEVDKNNINIFGEKNDREIKNNKNNKEIKIDIEIIKKIISYEIKAKYWEITGSFHWASMFDLKGNSIIFVEDIGRHNAVDKVIGYAILNNYNLNKLILRYSGRIPSDIVKKAINSGLNIIISKSPPTDKAIELAEENNILLIGFARNGKFNIYTSGRLWEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q57755</accession>
    <name>THIO_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trx</name>
        <name type="ordered locus">MJ0307</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Biochemistry" volume="39" first="6652" last="6659">
            <title>A thioredoxin from the hyperthermophilic archaeon Methanococcus jannaschii has a glutaredoxin-like fold but thioredoxin-like activities.</title>
            <authorList>
                <person name="Lee D.Y."/>
                <person name="Ahn B.-Y."/>
                <person name="Kim K.-S."/>
            </authorList>
            <dbReference type="PubMed" id="10828983"/>
            <dbReference type="DOI" id="10.1021/bi000035b"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>STRUCTURE BY NMR</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Acts to maintain redox homeostasis; functions as a protein disulfide reductase.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <redoxPotential>
            <text>E(0) is -277 mV.</text>
        </redoxPotential>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64338">
        <property type="entry name" value="D64338"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869805.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1FO5">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-85"/>
    </dbReference>
    <dbReference type="PDBsum" id="1FO5"/>
    <dbReference type="AlphaFoldDB" id="Q57755"/>
    <dbReference type="BMRB" id="Q57755"/>
    <dbReference type="SMR" id="Q57755"/>
    <dbReference type="STRING" id="243232.MJ_0307"/>
    <dbReference type="EnsemblBacteria" id="AAB98293">
        <property type="protein sequence ID" value="AAB98293"/>
        <property type="gene ID" value="MJ_0307"/>
    </dbReference>
    <dbReference type="GeneID" id="1451162"/>
    <dbReference type="KEGG" id="mja:MJ_0307"/>
    <dbReference type="eggNOG" id="arCOG01972">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_20_2_2"/>
    <dbReference type="InParanoid" id="Q57755"/>
    <dbReference type="OMA" id="YGIMAVP"/>
    <dbReference type="OrthoDB" id="110723at2157"/>
    <dbReference type="PhylomeDB" id="Q57755"/>
    <dbReference type="EvolutionaryTrace" id="Q57755"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004502">
        <property type="entry name" value="Thio_glut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00411">
        <property type="entry name" value="redox_disulf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0676">Redox-active center</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000141645" description="Thioredoxin">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaredoxin" evidence="1">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active">
        <location>
            <begin position="14"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="4"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="57"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="65"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="70"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10828983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1FO5"/>
        </source>
    </evidence>
    <sequence length="85" mass="9404" checksum="A38309843CD81A2D" modified="1996-11-01" version="1">MSKVKIELFTSPMCPHCPAAKRVVEEVANEMPDAVEVEYINVMENPQKAMEYGIMAVPTIVINGDVEFIGAPTKEALVEAIKKRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q57760</accession>
    <name>Y312_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0312</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0312</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64339">
        <property type="entry name" value="A64339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869810.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57760"/>
    <dbReference type="EnsemblBacteria" id="AAB98309">
        <property type="protein sequence ID" value="AAB98309"/>
        <property type="gene ID" value="MJ_0312"/>
    </dbReference>
    <dbReference type="GeneID" id="1451167"/>
    <dbReference type="KEGG" id="mja:MJ_0312"/>
    <dbReference type="eggNOG" id="arCOG08213">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1444656_0_0_2"/>
    <dbReference type="OMA" id="MSQAHEL"/>
    <dbReference type="OrthoDB" id="84242at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106788" description="Uncharacterized protein MJ0312">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <sequence length="206" mass="22367" checksum="1168AB576BFE033A" modified="1996-11-01" version="1">MSKGINPPKGGGTMEGRYMSQAHELLTNTGVENMANRTAERMIPLMNSLVTGYSIALAKTLGSGAGAMTQILLSEIGEVLSAMVDEILGSGQASYELENVEELLKNAFLELGIAKDVKIEKNIKDNMVIYKLYIKGSLFAPVHKILIDRGLKEFPLSPEGLLAASIVRRVLRERKDGNTKARINVNTKLPVNGETLIVEIKEVGSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57774</accession>
    <name>Y328_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0328</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0328</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64340">
        <property type="entry name" value="H64340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869825.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57774"/>
    <dbReference type="SMR" id="Q57774"/>
    <dbReference type="STRING" id="243232.MJ_0328"/>
    <dbReference type="EnsemblBacteria" id="AAB98316">
        <property type="protein sequence ID" value="AAB98316"/>
        <property type="gene ID" value="MJ_0328"/>
    </dbReference>
    <dbReference type="GeneID" id="1451183"/>
    <dbReference type="KEGG" id="mja:MJ_0328"/>
    <dbReference type="eggNOG" id="arCOG09635">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773192_0_0_2"/>
    <dbReference type="OMA" id="NKLCKVC"/>
    <dbReference type="OrthoDB" id="134721at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106797" description="Uncharacterized protein MJ0328">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <sequence length="146" mass="16795" checksum="418FFB0F00662027" modified="1996-11-01" version="1">MPNNTNNKLCKVCNSPHRAEIEALYFQGWGAKKISKYLKEKYNEDISYSAILRHMQNHVKPQLLEAIEEETTEIYSKMYKEIANNFGLALEGLFTMIKTAKKDLENPKATAREKEVAGRNLVMAIREMKELLQLTEDKEGADDIDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q57779</accession>
    <name>Y333_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0333</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0333</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64341">
        <property type="entry name" value="E64341"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869831.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57779"/>
    <dbReference type="SMR" id="Q57779"/>
    <dbReference type="STRING" id="243232.MJ_0333"/>
    <dbReference type="EnsemblBacteria" id="AAB98321">
        <property type="protein sequence ID" value="AAB98321"/>
        <property type="gene ID" value="MJ_0333"/>
    </dbReference>
    <dbReference type="GeneID" id="1451189"/>
    <dbReference type="KEGG" id="mja:MJ_0333"/>
    <dbReference type="eggNOG" id="arCOG09640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353231_0_0_2"/>
    <dbReference type="OMA" id="NGTSYHA"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106803" description="Uncharacterized protein MJ0333">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <sequence length="96" mass="10927" checksum="A4EB1A265B52EB99" modified="1996-11-01" version="1">MNKEKYGDNMMTDSDSKQAIFIIGVQGKEIKNVEQLMQELSKIVNEGSIYRYSKETGLGKGTLHKIKNNELQDPRISTVLKLLKASGKRLVIIDEW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57785</accession>
    <name>Y339_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0339</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0339</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64342">
        <property type="entry name" value="C64342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869837.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57785"/>
    <dbReference type="STRING" id="243232.MJ_0339"/>
    <dbReference type="EnsemblBacteria" id="AAB98327">
        <property type="protein sequence ID" value="AAB98327"/>
        <property type="gene ID" value="MJ_0339"/>
    </dbReference>
    <dbReference type="GeneID" id="1451195"/>
    <dbReference type="KEGG" id="mja:MJ_0339"/>
    <dbReference type="eggNOG" id="arCOG09644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2204112_0_0_2"/>
    <dbReference type="OMA" id="YTKLEFR"/>
    <dbReference type="OrthoDB" id="135979at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106809" description="Uncharacterized protein MJ0339">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <sequence length="107" mass="12450" checksum="9CC983A8F7F7596F" modified="1996-11-01" version="1">MKKRGRCSLTNYANAKALVEKILEDLKNNGIKVKSPLSKIQDFHCEADFSVEIENRVAYVDATFTFDKLPNEDLVEKIEAVMTTYNSYLERIDFESDYTKLEFRSVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q57826</accession>
    <name>CAS7_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated protein Cas7/Cst2/DevR</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR-associated protein Cas7/Csa2, subtype I-A/Apern</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cas7</name>
        <name type="ordered locus">MJ0381</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas7/Cst2/DevR family. Subtype I-a/Apern subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64347">
        <property type="entry name" value="E64347"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869880.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57826"/>
    <dbReference type="SMR" id="Q57826"/>
    <dbReference type="STRING" id="243232.MJ_0381"/>
    <dbReference type="PRIDE" id="Q57826"/>
    <dbReference type="EnsemblBacteria" id="AAB98370">
        <property type="protein sequence ID" value="AAB98370"/>
        <property type="gene ID" value="MJ_0381"/>
    </dbReference>
    <dbReference type="GeneID" id="1451238"/>
    <dbReference type="KEGG" id="mja:MJ_0381"/>
    <dbReference type="eggNOG" id="arCOG03617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054331_1_0_2"/>
    <dbReference type="InParanoid" id="Q57826"/>
    <dbReference type="OMA" id="MIYYVET"/>
    <dbReference type="OrthoDB" id="47264at2157"/>
    <dbReference type="PhylomeDB" id="Q57826"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002764">
        <property type="entry name" value="Cas7/Cst2/DevR_sub_I-a/Apern"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010154">
        <property type="entry name" value="CRISPR-assoc_Cas7/Cst2/DevR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01905">
        <property type="entry name" value="DevR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01875">
        <property type="entry name" value="cas_MJ0381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02583">
        <property type="entry name" value="DevR_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106844" description="CRISPR-associated protein Cas7/Cst2/DevR">
        <location>
            <begin position="1"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="320" mass="35924" checksum="81CA8DE178F4B223" modified="1996-11-01" version="1">MFLRISGRVRLNSHSLNAQGGGGTNYVEITKAKVSIKNDDRWEILEVPAISGNMVKHWHFVSFVDFFRETDYKDNLTERALRYNGARFGQETKAKKADGSEVELKDESEIIKNFADADVHGFLAPKTGVRRVSLVKTSFILPTEDFIKEVDERLVYAVKHNRVDIDEKGAIKSGEEQTAQMLFNREYATGLYGFEIILDLGFVGVPQSSPSNPVIEDDERKARIVSALKALIPMLSGYIGANLARSFPVFKLEEMIAVVSEKPIPALVHGFYEDYVEVSKNVVENAKKLGFEIEDFGYNVDFGESVSSVEELISKIIEKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q57840</accession>
    <name>RPO7_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 4">DNA-directed RNA polymerase subunit E</fullName>
            <shortName evidence="3">mjE</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit E'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo7</name>
        <name evidence="1" type="synonym">rpoE</name>
        <name evidence="5" type="synonym">rpoE1</name>
        <name type="ordered locus">MJ0397</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Nucleic Acids Res." volume="28" first="4299" last="4305">
            <title>Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12.</title>
            <authorList>
                <person name="Werner F."/>
                <person name="Eloranta J.J."/>
                <person name="Weinzierl R.O."/>
            </authorList>
            <dbReference type="PubMed" id="11058130"/>
            <dbReference type="DOI" id="10.1093/nar/28.21.4299"/>
        </citation>
        <scope>INTERACTION WITH RPO4</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="journal article" date="2001" name="Mol. Cell" volume="8" first="1137" last="1143">
            <title>Structure of an archaeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex.</title>
            <authorList>
                <person name="Todone F."/>
                <person name="Brick P."/>
                <person name="Werner F."/>
                <person name="Weinzierl R.O."/>
                <person name="Onesti S."/>
            </authorList>
            <dbReference type="PubMed" id="11741548"/>
            <dbReference type="DOI" id="10.1016/s1097-2765(01)00379-3"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS)</scope>
        <scope>DOMAIN</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 6">Part of the RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1 2">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64349">
        <property type="entry name" value="E64349"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869896.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1GO3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="E/M=1-187"/>
    </dbReference>
    <dbReference type="PDBsum" id="1GO3"/>
    <dbReference type="AlphaFoldDB" id="Q57840"/>
    <dbReference type="SMR" id="Q57840"/>
    <dbReference type="IntAct" id="Q57840">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0397"/>
    <dbReference type="EnsemblBacteria" id="AAB98387">
        <property type="protein sequence ID" value="AAB98387"/>
        <property type="gene ID" value="MJ_0397"/>
    </dbReference>
    <dbReference type="GeneID" id="1451254"/>
    <dbReference type="KEGG" id="mja:MJ_0397"/>
    <dbReference type="eggNOG" id="arCOG00675">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117966_0_0_2"/>
    <dbReference type="InParanoid" id="Q57840"/>
    <dbReference type="OMA" id="MRQPGLG"/>
    <dbReference type="OrthoDB" id="117241at2157"/>
    <dbReference type="PhylomeDB" id="Q57840"/>
    <dbReference type="EvolutionaryTrace" id="Q57840"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="PTHR12709"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="SSF88798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000074036" description="DNA-directed RNA polymerase subunit Rpo7">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif" evidence="1">
        <location>
            <begin position="82"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="2"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="102"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="127"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="160"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11741548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="11741548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="8688087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="1GO3"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="1GO3"/>
        </source>
    </evidence>
    <sequence length="187" mass="21216" checksum="C0E4EA3C006D69DB" modified="1997-11-01" version="1">MYKILEIADVVKVPPEEFGKDLKETVKKILMEKYEGRLDKDVGFVLSIVDVKDIGEGKVVHGDGSAYHPVVFETLVYIPEMYELIEGEVVDVVEFGSFVRLGPLDGLIHVSQIMDDYVSYDPKREAIIGKETGKVLEIGDYVRARIVAISLKAERKRGSKIALTMRQPYLGKLEWIEEEKAKKQNQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57848</accession>
    <name>Y405_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0405</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0405</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64350">
        <property type="entry name" value="E64350"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57848"/>
    <dbReference type="STRING" id="243232.MJ_0405"/>
    <dbReference type="EnsemblBacteria" id="AAB98392">
        <property type="protein sequence ID" value="AAB98392"/>
        <property type="gene ID" value="MJ_0405"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0405"/>
    <dbReference type="eggNOG" id="arCOG04902">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147797_0_0_2"/>
    <dbReference type="InParanoid" id="Q57848"/>
    <dbReference type="OMA" id="TPICGPY"/>
    <dbReference type="PhylomeDB" id="Q57848"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012029">
        <property type="entry name" value="UCP006557"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09884">
        <property type="entry name" value="DUF2111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006557">
        <property type="entry name" value="UCP006557_sign"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106856" description="Uncharacterized protein MJ0405">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="131" mass="14224" checksum="2849EAA7A9851B82" modified="1996-11-01" version="1">MKIMITISENSEAKELMPIAQAVHILVNKLPVAMRSKNKPGVRLEKGEVVDTNYEGYVLKVAIEKGEVVRATPIIGPYAGLPVIVAPIKDGDNVLGAIGVVDITAGIFEDIVAISRRPELYKFLPEDAFPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57857</accession>
    <name>Y414_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0414</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0414</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64351">
        <property type="entry name" value="F64351"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57857"/>
    <dbReference type="SMR" id="Q57857"/>
    <dbReference type="STRING" id="243232.MJ_0414"/>
    <dbReference type="EnsemblBacteria" id="AAB98403">
        <property type="protein sequence ID" value="AAB98403"/>
        <property type="gene ID" value="MJ_0414"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0414"/>
    <dbReference type="eggNOG" id="arCOG04218">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061502_0_0_2"/>
    <dbReference type="InParanoid" id="Q57857"/>
    <dbReference type="OMA" id="ICPFTTK"/>
    <dbReference type="PhylomeDB" id="Q57857"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07894">
        <property type="entry name" value="Adenylation_RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041596">
        <property type="entry name" value="Lig_Pab1020_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001072">
        <property type="entry name" value="RNA_ligase_Pab1020"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18330">
        <property type="entry name" value="Lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01048">
        <property type="entry name" value="Y414FAMILY"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01209">
        <property type="entry name" value="TIGR01209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106860" description="Uncharacterized protein MJ0414">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <sequence length="395" mass="46417" checksum="47AD62037D44D33B" modified="1996-11-01" version="1">MRLIMMKVSAYDLNKIAEKLNLSIKDLNKAFSRKILREDEYKEIKTLLFKKEFKGIEKGTVIFLNDNLDVVRGYPKTYRAITLYPTIKKHFIDKVVIEEKLNGYNIRIVKIDGEVYALTRSGYICPFTTKKVKKFLNLEILDDYSEYMLCGEMIGINNPYTPYYYKEVDRGFENLGFYIFDIKERETNKSLPIKERINLCEKYNLPYVKPLAVVDKDEAHIHVREIIEKLNKEGREGVVLKDPDMAVSPIKYTTHYTQCEDLKSAFTFFFDLGMDFLFSRVVREGFMSYEFKETLEERKNRAKDLGEAILLPMVETINKVASGERVSEDFELIFDSEEDFDEFLDFMRKMKMVITIKNIEKIDTEEGVKIKAVIGKIYNKTNDKIISYLNGTLWE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q57861</accession>
    <name>Y418_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0418</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0418</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64352">
        <property type="entry name" value="B64352"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57861"/>
    <dbReference type="SMR" id="Q57861"/>
    <dbReference type="STRING" id="243232.MJ_0418"/>
    <dbReference type="EnsemblBacteria" id="AAB98416">
        <property type="protein sequence ID" value="AAB98416"/>
        <property type="gene ID" value="MJ_0418"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0418"/>
    <dbReference type="eggNOG" id="arCOG03414">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1105215_0_0_2"/>
    <dbReference type="InParanoid" id="Q57861"/>
    <dbReference type="OMA" id="EMYKCAW"/>
    <dbReference type="PhylomeDB" id="Q57861"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106864" description="Uncharacterized protein MJ0418">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="257" mass="28765" checksum="CFEAA1AA6AB38000" modified="1998-07-15" version="3">MIIMKKIGILEIVVILSILITSVSLAYKFYSNNGNDYEFDGNQMYKCAWVCEKILNKNFPLNATIIGKWTLSKKPFNGEVKIYDAKGGTLYAIYNGTPITIGGELAYQEDIAAKKIILHPIGKSIIFYELNPIEGKSFRDIANEIENTTKNFNGLNIVDVIVEGSMGVDSKTYTPVERQKIMNNLDVDIKKGLGLYFVDYGIIINGKIHLNTLKNLDNYINSSNISTSKLTIYVVVNNSIDEIPNKIKENYAIITLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q57889</accession>
    <name>Y447_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0447</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0447</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64355">
        <property type="entry name" value="G64355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869946.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57889"/>
    <dbReference type="SMR" id="Q57889"/>
    <dbReference type="STRING" id="243232.MJ_0447"/>
    <dbReference type="EnsemblBacteria" id="AAB98447">
        <property type="protein sequence ID" value="AAB98447"/>
        <property type="gene ID" value="MJ_0447"/>
    </dbReference>
    <dbReference type="GeneID" id="1451307"/>
    <dbReference type="KEGG" id="mja:MJ_0447"/>
    <dbReference type="eggNOG" id="arCOG09632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656864_0_0_2"/>
    <dbReference type="OMA" id="NREWRWK"/>
    <dbReference type="OrthoDB" id="82299at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106879" description="Uncharacterized protein MJ0447">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <sequence length="161" mass="19125" checksum="13DDED5CC33A10BB" modified="1996-11-01" version="1">MNLNIKEIKQKINEWKNREWRWKGKGKIEIRFVCLIERAESFKELVDNLEIIICEYEKIKQLIEDKDIKEIAKLNLFCGNNVYEEMLKDILSSNKFISLTISFDENIAYVKYMERGKEEVVYLDGKSAYKALQILKNRYENILKKQISIIEDAIPLTIPSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q57904</accession>
    <name>Y480_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0480</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0480</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64359">
        <property type="entry name" value="H64359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869981.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="5C8J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="I/J/K/L=1-198"/>
    </dbReference>
    <dbReference type="PDBsum" id="5C8J"/>
    <dbReference type="AlphaFoldDB" id="Q57904"/>
    <dbReference type="SMR" id="Q57904"/>
    <dbReference type="STRING" id="243232.MJ_0480"/>
    <dbReference type="ABCD" id="Q57904">
        <property type="antibodies" value="1 sequenced antibody"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98471">
        <property type="protein sequence ID" value="AAB98471"/>
        <property type="gene ID" value="MJ_0480"/>
    </dbReference>
    <dbReference type="GeneID" id="1451342"/>
    <dbReference type="KEGG" id="mja:MJ_0480"/>
    <dbReference type="eggNOG" id="arCOG02673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121274_0_0_2"/>
    <dbReference type="InParanoid" id="Q57904"/>
    <dbReference type="OMA" id="LGWYILC"/>
    <dbReference type="OrthoDB" id="94214at2157"/>
    <dbReference type="PhylomeDB" id="Q57904"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="DisProt" id="DP01527"/>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="PTHR42198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106888" description="Uncharacterized protein MJ0480">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="198" mass="23072" checksum="91CFB738E3E6BAC8" modified="1996-11-01" version="1">MFGSIFDIYYKTLDAIFMPIIKVLHPALAILIIAIIVSLIINIATKLLVDQKRVAELKKEIQEFQVKFKKMSKNPEMMEKLQEEQQRIMQLNAELMKMSFRPMIYTWVPIILIFIYLRHVYGFGGVYQELNPGWNGVVVYLPIILSKILFIDFWHWLGSIFYKGGFKIVSNTALGWLGWYILCSFATSTVLRKILGIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q57928</accession>
    <name>Y505_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0505</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0505</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64363">
        <property type="entry name" value="A64363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496918.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57928"/>
    <dbReference type="SMR" id="Q57928"/>
    <dbReference type="STRING" id="243232.MJ_0505"/>
    <dbReference type="EnsemblBacteria" id="AAB98501">
        <property type="protein sequence ID" value="AAB98501"/>
        <property type="gene ID" value="MJ_0505"/>
    </dbReference>
    <dbReference type="GeneID" id="1451367"/>
    <dbReference type="KEGG" id="mja:MJ_0505"/>
    <dbReference type="eggNOG" id="arCOG05033">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078976_0_0_2"/>
    <dbReference type="InParanoid" id="Q57928"/>
    <dbReference type="OMA" id="YFIPPRY"/>
    <dbReference type="PhylomeDB" id="Q57928"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018763">
        <property type="entry name" value="DUF2334"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10096">
        <property type="entry name" value="DUF2334"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106904" description="Uncharacterized protein MJ0505">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="250" mass="29848" checksum="5A645296E7E2E153" modified="1997-11-01" version="2">MNKFKYLLFLVVFAVFFLTFAFFDNSSKSHQDDDEQKPIILIHDVSPVYFKELKEIVKIIDKYHYQNRSYLFLIVNHANKYNLKNYPEFVDYLHKLEKEGYHIEFHAYNHIDDEFNCNKTVAEEKLNKSFKILEECGFNPKKIKYFIPPRYKLSEDAEKLFLGRNITIILENKMITEKDGKIVEISITNREYTWYLPKPLVKVAEKIATTDYKLSIKENRKFFLSIHPKAVNYGSGLEFLDYFLNETSKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q57981</accession>
    <name>PURA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenylosuccinate synthetase</fullName>
            <shortName evidence="1">AMPSase</shortName>
            <shortName evidence="1">AdSS</shortName>
            <ecNumber evidence="1">6.3.4.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">IMP--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">purA</name>
        <name type="ordered locus">MJ0561</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + IMP + L-aspartate = GDP + 2 H(+) + N(6)-(1,2-dicarboxyethyl)-AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.3.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylosuccinate synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.4" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64370">
        <property type="entry name" value="A64370"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57981"/>
    <dbReference type="SMR" id="Q57981"/>
    <dbReference type="STRING" id="243232.MJ_0561"/>
    <dbReference type="EnsemblBacteria" id="AAB98554">
        <property type="protein sequence ID" value="AAB98554"/>
        <property type="gene ID" value="MJ_0561"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0561"/>
    <dbReference type="eggNOG" id="arCOG04387">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029848_0_0_2"/>
    <dbReference type="InParanoid" id="Q57981"/>
    <dbReference type="OMA" id="FHHAKPI"/>
    <dbReference type="PhylomeDB" id="Q57981"/>
    <dbReference type="BRENDA" id="6.3.4.4">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00335"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004019">
        <property type="term" value="F:adenylosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046040">
        <property type="term" value="P:IMP metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03108">
        <property type="entry name" value="AdSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.300.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.440.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.170.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00011">
        <property type="entry name" value="Adenylosucc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018220">
        <property type="entry name" value="Adenylosuccin_syn_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042109">
        <property type="entry name" value="Adenylosuccinate_synth_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042110">
        <property type="entry name" value="Adenylosuccinate_synth_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042111">
        <property type="entry name" value="Adenylosuccinate_synth_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001114">
        <property type="entry name" value="Adenylosuccinate_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11846">
        <property type="entry name" value="PTHR11846"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00709">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00788">
        <property type="entry name" value="Adenylsucc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01266">
        <property type="entry name" value="ADENYLOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000095271" description="Adenylosuccinate synthetase">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="18"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="19"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="258"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="290"/>
            <end position="292"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="330"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00011"/>
        </source>
    </evidence>
    <sequence length="345" mass="37820" checksum="9974DBC30D1DEE72" modified="1996-11-01" version="1">MKKVVLLTCTIIVGGQWGDEGKGKIISYICDKDKPSIIARGGVGPNAGHTVNIGGKSYGIRMIPTGFPYKEAKLAIGAGVLVDPEVLLKEVEMLKDFNVKERLIVDYRCGIIEEKHKIMDRKDEHLAKEIGTTGSGCGPANVDRVLRILKQAKDIEELKEFLGDVSEEVNNALDRGENVLIEGTQGTLLSLYYGTYPYVTSKDTTASSFAADVGIGPTKVDEVIVVFKTFPTRVGAGPFPTEMSLEEAESLGIVEYGTVTGRRRRVGYFDFELARKACRLNGATQIALTGLDKYDKECYGVTEYNKLSEKAKEFINKIEEVTGVPVTIISTGPEMHQTIDLRNEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q57982</accession>
    <name>Y562_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0562</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0562</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64370">
        <property type="entry name" value="B64370"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57982"/>
    <dbReference type="SMR" id="Q57982"/>
    <dbReference type="EnsemblBacteria" id="AAB98560">
        <property type="protein sequence ID" value="AAB98560"/>
        <property type="gene ID" value="MJ_0562"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0562"/>
    <dbReference type="HOGENOM" id="CLU_2678943_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106932" description="Uncharacterized protein MJ0562">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <sequence length="74" mass="8770" checksum="8A99FF0EF7017898" modified="1996-11-01" version="1">MIGMNFKDPIEELLDNYFNAKKEYEKNPIEKNLNRLKKAEAKLMINYPNTNATYIYKNKKYKIIIKDSVSVIPI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58002</accession>
    <name>Y582_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0582</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0582</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0766.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64372">
        <property type="entry name" value="F64372"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870086.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58002"/>
    <dbReference type="STRING" id="243232.MJ_0582"/>
    <dbReference type="DNASU" id="1451447"/>
    <dbReference type="EnsemblBacteria" id="AAB98576">
        <property type="protein sequence ID" value="AAB98576"/>
        <property type="gene ID" value="MJ_0582"/>
    </dbReference>
    <dbReference type="GeneID" id="1451447"/>
    <dbReference type="KEGG" id="mja:MJ_0582"/>
    <dbReference type="eggNOG" id="arCOG02639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_147304_3_0_2"/>
    <dbReference type="InParanoid" id="Q58002"/>
    <dbReference type="OMA" id="SCFKAIN"/>
    <dbReference type="OrthoDB" id="91651at2157"/>
    <dbReference type="PhylomeDB" id="Q58002"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014925">
        <property type="entry name" value="CGGC_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08821">
        <property type="entry name" value="CGGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01078">
        <property type="entry name" value="CGGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106942" description="Uncharacterized protein MJ0582">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="128" mass="14086" checksum="B39A14381AA19EA0" modified="1996-11-01" version="1">MKVAIIACQKMVEMGCPGKEACVSCFKAINEKSGAFERYKDVELVAFTTCGGCPGRRFPMRVKLLKTAAGAEAIHIANCTFLQPECPYINFDEICKKLMEELEIPIVFGTHTLVKKGEVVCTCGDNKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58015</accession>
    <name>MTM3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type II methyltransferase M.MjaIII</fullName>
            <shortName evidence="2">M.MjaIII</shortName>
            <ecNumber>2.1.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Adenine-specific methyltransferase MjaIII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaIIIM</name>
        <name type="ordered locus">MJ0598</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">An alpha subtype methylase that recognizes the double-stranded sequence 5'-GATC-3', methylates A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72"/>
    <dbReference type="EMBL" id="L77117">
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            <position position="9"/>
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        <location>
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        </location>
        <ligand>
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            <position position="199"/>
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        <ligand>
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        </ligand>
    </feature>
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            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
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</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q58024</accession>
    <name>Y607_METJA</name>
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            <fullName>Uncharacterized protein MJ0607</fullName>
        </recommendedName>
    </protein>
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        <name type="ordered locus">MJ0607</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
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            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
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            <taxon>Methanocaldococcus</taxon>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
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            <end position="79"/>
        </location>
    </feature>
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</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58034</accession>
    <name>TTDB_METJA</name>
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        <recommendedName>
            <fullName evidence="1">Putative L(+)-tartrate dehydratase subunit beta</fullName>
            <shortName evidence="1">L-TTD beta</shortName>
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        </recommendedName>
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        <name type="ordered locus">MJ0617</name>
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    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
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            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <reaction evidence="1">
            <text>(2R,3R)-tartrate = H2O + oxaloacetate</text>
            <dbReference type="Rhea" id="RHEA:15413"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
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            <dbReference type="EC" id="4.2.1.32"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I fumarase family.</text>
    </comment>
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        <property type="resolution" value="2.30 A"/>
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    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008730">
        <property type="term" value="F:L(+)-tartrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="Gene3D" id="3.20.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004647">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdB-typ_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036660">
        <property type="entry name" value="Fe-S_hydroAse_TtdB_cat_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05683">
        <property type="entry name" value="Fumerase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117457">
        <property type="entry name" value="SSF117457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00723">
        <property type="entry name" value="ttdB_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000195662" description="Putative L(+)-tartrate dehydratase subunit beta">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="36"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
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            <end position="7"/>
        </location>
    </feature>
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    </feature>
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    <feature type="strand" evidence="4">
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        <source>
            <dbReference type="PDB" id="5DNI"/>
        </source>
    </evidence>
    <sequence length="195" mass="21868" checksum="A5FDEB0E77D00902" modified="1996-11-01" version="1">MEYTFNKLTKKDVKKLKVGDIVYLNGKIYTARDEAHLKIIEMLKSNEKLPFDLNESIIYHAGPIMKKVNDSWVCVSIGPTTSARMNDVEEEFIKLTNISAIVGKGGMKKELLKTFEDYGVVYLAAPGGCAALLANSVKRVDNVYFLDELGMPEAVWELEVNNFGPLIVAMDSHGNSIYEEVNKKVYEKLNELIGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58038</accession>
    <name>Y621_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0621</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0621</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64377">
        <property type="entry name" value="E64377"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870126.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58038"/>
    <dbReference type="SMR" id="Q58038"/>
    <dbReference type="STRING" id="243232.MJ_0621"/>
    <dbReference type="EnsemblBacteria" id="AAB98616">
        <property type="protein sequence ID" value="AAB98616"/>
        <property type="gene ID" value="MJ_0621"/>
    </dbReference>
    <dbReference type="GeneID" id="1451487"/>
    <dbReference type="KEGG" id="mja:MJ_0621"/>
    <dbReference type="eggNOG" id="arCOG04375">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_129113_0_0_2"/>
    <dbReference type="InParanoid" id="Q58038"/>
    <dbReference type="OMA" id="REYSATS"/>
    <dbReference type="OrthoDB" id="99300at2157"/>
    <dbReference type="PhylomeDB" id="Q58038"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016472">
        <property type="entry name" value="Tscrpt_reg_MJ0621_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005978">
        <property type="entry name" value="HTH_MJ0621_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000149792" description="Uncharacterized HTH-type transcriptional regulator MJ0621">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00257"/>
        </source>
    </evidence>
    <sequence length="172" mass="19263" checksum="4519725AD4020031" modified="1996-11-01" version="1">MKACERLLLKIESQEKFVEEFKRILLELGLTLKEFSEISGIPYSTLYKVIQGKDFRVSTLIKILKTIRSFEKDENIDTIAIIAARPALNKITTRKIGINGKSYLIKEYPANSLEECIVAAVRAEREGVKGIVCAPIVSATIEKIVNVPVAVIIPEKDAFMKALEIIAKKINE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58056</accession>
    <name>Y639_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0639</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0639</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64379">
        <property type="entry name" value="G64379"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58056"/>
    <dbReference type="STRING" id="243232.MJ_0639"/>
    <dbReference type="EnsemblBacteria" id="AAB98635">
        <property type="protein sequence ID" value="AAB98635"/>
        <property type="gene ID" value="MJ_0639"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0639"/>
    <dbReference type="eggNOG" id="arCOG05044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2353229_0_0_2"/>
    <dbReference type="OMA" id="CYYRSGF"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106966" description="Uncharacterized protein MJ0639">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <sequence length="109" mass="12890" checksum="8364B35BD3C629EF" modified="1996-11-01" version="1">MWLLIFNIIFNGETMKLAVDAVFYVREGFNFEKAFKEVLKILGEDVKILSVEYPELALISENGYYYRCGFMLDKELREELSGEEINKIKEKIKKLFEDEIIYTLTCEIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58118</accession>
    <name>TSR3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="2">2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0708</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine at position 914 in 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>an N(1)-methylpseudouridine in 16S/18S rRNA + S-adenosyl-L-methionine = H(+) + N(1)-methyl-N(3)[(3S)-3-amino-3-carboxypropyl]pseudouridine in 16S/18S rRNA + S-methyl-5'-thioadenosine</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64388">
        <property type="entry name" value="D64388"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58118"/>
    <dbReference type="SMR" id="Q58118"/>
    <dbReference type="STRING" id="243232.MJ_0708"/>
    <dbReference type="EnsemblBacteria" id="AAB98702">
        <property type="protein sequence ID" value="AAB98702"/>
        <property type="gene ID" value="MJ_0708"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0708"/>
    <dbReference type="eggNOG" id="arCOG04733">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035060_4_2_2"/>
    <dbReference type="InParanoid" id="Q58118"/>
    <dbReference type="OMA" id="DCSWESA"/>
    <dbReference type="PhylomeDB" id="Q58118"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:18S rRNA aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="PTHR20426"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000094418" description="16S rRNA aminocarboxypropyltransferase">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="E1QU22"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="172" mass="20030" checksum="01CF25475AD10B2A" modified="1996-11-01" version="1">MITMPKLFIYHANQCNPKKCTSLKMAKMNKAILLKNPYKVPKNSLILNPYAEKALSPEDKEIVEKFGITALDCSWKEAELMFKKFKFKNQRSLPFLVACNPINYGKPCMLSTLEAFIAALYITNFKDEAWDLTSCFKWAETFIKVNYELLERYSNAKNSMEVVEIQQDFLRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58146</accession>
    <name>TDXH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peroxiredoxin</fullName>
            <ecNumber evidence="1">1.11.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Thioredoxin peroxidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thioredoxin-dependent peroxiredoxin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0736</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[thioredoxin]-dithiol + a hydroperoxide = [thioredoxin]-disulfide + an alcohol + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodecamer. Pentamer of dimers that assemble into a ring structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. Although the primary sequence of this enzyme is similar to those of the 1-Cys Prx6 enzymes, its catalytic properties resemble those of the typical 2-Cys Prxs and C(R) is provided by the other dimeric subunit to form an intersubunit disulfide. The disulfide is subsequently reduced by thioredoxin.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. Prx6 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98731" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98731.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64391">
        <property type="entry name" value="H64391"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58146"/>
    <dbReference type="SMR" id="Q58146"/>
    <dbReference type="STRING" id="243232.MJ_0736"/>
    <dbReference type="EnsemblBacteria" id="AAB98731">
        <property type="protein sequence ID" value="AAB98731"/>
        <property type="gene ID" value="MJ_0736"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0736"/>
    <dbReference type="eggNOG" id="arCOG00312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042529_4_4_2"/>
    <dbReference type="InParanoid" id="Q58146"/>
    <dbReference type="OMA" id="FWYPKDF"/>
    <dbReference type="PhylomeDB" id="Q58146"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03016">
        <property type="entry name" value="PRX_1cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00401">
        <property type="entry name" value="Peroxiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024706">
        <property type="entry name" value="Peroxiredoxin_AhpC-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022915">
        <property type="entry name" value="Peroxiredoxin_TDXH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045020">
        <property type="entry name" value="PRX_1cys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000239">
        <property type="entry name" value="AHPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0049">Antioxidant</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0575">Peroxidase</keyword>
    <keyword id="KW-0676">Redox-active center</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000135155" description="Peroxiredoxin">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="1">
        <location>
            <begin position="2"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine sulfenic acid (-SOH) intermediate" evidence="1">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="disulfide bond" description="Interchain (with C-212); in linked form" evidence="1">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Alternate" evidence="1">
        <location>
            <begin position="206"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (with C-46); in linked form" evidence="1">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="217" mass="25010" checksum="6D64B6CF1BC0CAA5" modified="2001-06-01" version="2">MPVIGEKFPEVEVKTTHGAIKLPDYYVEKGKWFVLFSHPADFTPVCTTEFVGFQKRYDEFRKLNTELIGLSIDQVFSHLKWVEWIKEKLNVEIEFPIIADDRGELAEKLGMISPYKGNNTVRAVFVVDNKGIIRAIIYYPQEVGRNLDEIVRLVKALQVSDEKGVAMPANWPENDLIGDKVIIPPASSVEEIKQRKEACEKGEIECLDWWFCYKKLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58152</accession>
    <name>Y742_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0742</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0742</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1511.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64392">
        <property type="entry name" value="F64392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870247.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3D7I">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A/B/C=1-104"/>
    </dbReference>
    <dbReference type="PDBsum" id="3D7I"/>
    <dbReference type="AlphaFoldDB" id="Q58152"/>
    <dbReference type="SMR" id="Q58152"/>
    <dbReference type="STRING" id="243232.MJ_0742"/>
    <dbReference type="EnsemblBacteria" id="AAB98736">
        <property type="protein sequence ID" value="AAB98736"/>
        <property type="gene ID" value="MJ_0742"/>
    </dbReference>
    <dbReference type="GeneID" id="1451619"/>
    <dbReference type="KEGG" id="mja:MJ_0742"/>
    <dbReference type="eggNOG" id="arCOG02148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_5_0_2"/>
    <dbReference type="OMA" id="VASKCDE"/>
    <dbReference type="OrthoDB" id="108307at2157"/>
    <dbReference type="PhylomeDB" id="Q58152"/>
    <dbReference type="EvolutionaryTrace" id="Q58152"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107011" description="Uncharacterized protein MJ0742">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="9"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="75"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3D7I"/>
        </source>
    </evidence>
    <sequence length="104" mass="11703" checksum="AD2E2BED3D563A82" modified="1996-11-01" version="1">MKNEVFFGEGMKVVKEKYPDLYDIIVKLNDTVFTGKTLDYKTQKLIAIGIVASRCDEVAIEKQMKSAMKELGITKEEIADVLRVVLLTSGMPAFTKAMKILEKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58186</accession>
    <name>Y776_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0776</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0776</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64396">
        <property type="entry name" value="H64396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870281.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58186"/>
    <dbReference type="SMR" id="Q58186"/>
    <dbReference type="STRING" id="243232.MJ_0776"/>
    <dbReference type="EnsemblBacteria" id="AAB98766">
        <property type="protein sequence ID" value="AAB98766"/>
        <property type="gene ID" value="MJ_0776"/>
    </dbReference>
    <dbReference type="GeneID" id="1451653"/>
    <dbReference type="KEGG" id="mja:MJ_0776"/>
    <dbReference type="eggNOG" id="arCOG01595">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057102_0_0_2"/>
    <dbReference type="InParanoid" id="Q58186"/>
    <dbReference type="OMA" id="FLCFNKQ"/>
    <dbReference type="OrthoDB" id="44157at2157"/>
    <dbReference type="PhylomeDB" id="Q58186"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-18797"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016677">
        <property type="entry name" value="UCP016817_carboligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016817">
        <property type="entry name" value="UCP016817_carboligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107027" description="Uncharacterized protein MJ0776">
        <location>
            <begin position="1"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="131"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="383" mass="43948" checksum="FD11A7D7E8C241AC" modified="1996-11-01" version="1">MNNFTLFLFSCLYFIGGNLKALVLGINTRPVVNSLKKLGFYVYSVSYYAPEDLNADEKYYLINPLVHGRLKENYDENKLIEIANKLADEVDCIFITSGVFEFENSKIPGWDNVIGNGPKKINEISNKYKTYKKLKNLGFNIPETKKINNKTQLYKFLEEFKTCILKPIYGSGGSILKIELNNFDDEIINEIKFPIIAQEYIRGKSFSANFIGNTFITFNKQIIIKGMYAGNLTPYINLPNKFVEIFGEVIESFELKGMSGIDFLIKDNGPYIVDINPRILGTYETIEMSASQNLAMVLLNNKYAKEIKPRKVYIKRILFAKEKIIANISKRDFIHDIPKKNAVIEKGEPIATVIAKENIKSIINSVYEECAEYEKRKEDRENI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58196</accession>
    <name>Y786_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0786</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0786</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64398">
        <property type="entry name" value="B64398"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58196"/>
    <dbReference type="SMR" id="Q58196"/>
    <dbReference type="STRING" id="243232.MJ_0786"/>
    <dbReference type="EnsemblBacteria" id="AAB98786">
        <property type="protein sequence ID" value="AAB98786"/>
        <property type="gene ID" value="MJ_0786"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0786"/>
    <dbReference type="eggNOG" id="arCOG02614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1405969_0_0_2"/>
    <dbReference type="InParanoid" id="Q58196"/>
    <dbReference type="OMA" id="ILIIPDY"/>
    <dbReference type="PhylomeDB" id="Q58196"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107036" description="Uncharacterized protein MJ0786">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <sequence length="186" mass="21628" checksum="769D5413D99977F7" modified="1996-11-01" version="1">MIRGTMKIGITKMYKEIADLIGLEEYEIVNPYNTKVDCDFLIISKGYKERVKKLNPESEIFEVKSATFMDLIKSLEELKKLGIGKEGKIDKSIEFLKNKEKEIKKLVKDLNVKVNPKTEFIRKVVEDLGLEISDNGILIIPDYLFDGKNIENIIILKTHNYDLGLVERIEDRYLQIIQSIQKYLNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58202</accession>
    <name>Y792_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0792</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0792</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64398">
        <property type="entry name" value="H64398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870299.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58202"/>
    <dbReference type="STRING" id="243232.MJ_0792"/>
    <dbReference type="EnsemblBacteria" id="AAB98789">
        <property type="protein sequence ID" value="AAB98789"/>
        <property type="gene ID" value="MJ_0792"/>
    </dbReference>
    <dbReference type="GeneID" id="1451671"/>
    <dbReference type="KEGG" id="mja:MJ_0792"/>
    <dbReference type="eggNOG" id="arCOG09673">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2447691_0_0_2"/>
    <dbReference type="OrthoDB" id="131582at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107042" description="Uncharacterized protein MJ0792">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="89" mass="9084" checksum="06D02F0D9ED983BB" modified="1996-11-01" version="1">MKFLEKGVYKIFGAVILVSMIGALVAEPIALGDAGLYYQYYVGDIDTAQQCYLVAADSAITGAAISAALGPAGLASGVFTVVFSTTVLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58212</accession>
    <name>Y802_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0802</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0802</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64400">
        <property type="entry name" value="B64400"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870312.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58212"/>
    <dbReference type="STRING" id="243232.MJ_0802"/>
    <dbReference type="EnsemblBacteria" id="AAB98803">
        <property type="protein sequence ID" value="AAB98803"/>
        <property type="gene ID" value="MJ_0802"/>
    </dbReference>
    <dbReference type="GeneID" id="1451684"/>
    <dbReference type="KEGG" id="mja:MJ_0802"/>
    <dbReference type="eggNOG" id="arCOG04904">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121275_0_0_2"/>
    <dbReference type="InParanoid" id="Q58212"/>
    <dbReference type="OMA" id="FIFVASE"/>
    <dbReference type="OrthoDB" id="93447at2157"/>
    <dbReference type="PhylomeDB" id="Q58212"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016762">
        <property type="entry name" value="UCP019464_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09886">
        <property type="entry name" value="DUF2113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019464">
        <property type="entry name" value="UCP019464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03291">
        <property type="entry name" value="methan_mark_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107052" description="Uncharacterized protein MJ0802">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <sequence length="188" mass="21768" checksum="D651D0C61F9D1028" modified="1996-11-01" version="1">MAEIVVHCEDKAGKEIYTKVIQTALEDLLLGKSIIRVEFIAKEKEPYFILGVLPKHTRRAIKLRDFAEIVEQKKEGGKIIYKLKITDETYLPHLLKKIHVIDQPSRFEVVTDSDIDLDMEVYDAGKDFIDKVMDFMGRVFPEGMRIKNTYIDKAIVCIASERPLKDEEIEEALKLKDKLEKMNTAGYY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q58243</accession>
    <name>Y833_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0833</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0833</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64404">
        <property type="entry name" value="A64404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870345.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58243"/>
    <dbReference type="STRING" id="243232.MJ_0833"/>
    <dbReference type="EnsemblBacteria" id="AAB98836">
        <property type="protein sequence ID" value="AAB98836"/>
        <property type="gene ID" value="MJ_0833"/>
    </dbReference>
    <dbReference type="GeneID" id="1451717"/>
    <dbReference type="KEGG" id="mja:MJ_0833"/>
    <dbReference type="eggNOG" id="arCOG05054">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_978658_0_0_2"/>
    <dbReference type="OMA" id="WIPPYTT"/>
    <dbReference type="OrthoDB" id="56925at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107068" description="Uncharacterized protein MJ0833">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <sequence length="277" mass="31995" checksum="5EFCF35AD3AB11F8" modified="1998-12-15" version="2">MFMKSLIKLIVFIVLCSLFLHSICGERTIAEMSITYKLTGEITNTNPYSIFVAVPSNITFEEKTLPKPEDFLDVSTSVTQTSGIVFYKTIFNGKEGFWIPPYTTVKINIYHYTPITYDIKIDESQENYDVVGPAVVNKVNVIDLNKLFPDAKYEGIKIGKFKLYVSGYIVKGNDTESLSIIVPAPLVIDNYDEFHKFGDDNVDIWISSYNEWYKNQMERENIHIDNNDPLIPKMDNDVLGDDTHFKFKIFDVPAMAFTTSSNQPIRFYYIIYYKYNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58259</accession>
    <name>MTRC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tetrahydromethanopterin S-methyltransferase subunit C</fullName>
            <ecNumber>2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mtrC</name>
        <name type="ordered locus">MJ0849</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MtrC family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64406">
        <property type="entry name" value="A64406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870363.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58259"/>
    <dbReference type="STRING" id="243232.MJ_0849"/>
    <dbReference type="DNASU" id="1451737"/>
    <dbReference type="EnsemblBacteria" id="AAB98854">
        <property type="protein sequence ID" value="AAB98854"/>
        <property type="gene ID" value="MJ_0849"/>
    </dbReference>
    <dbReference type="GeneID" id="1451737"/>
    <dbReference type="KEGG" id="mja:MJ_0849"/>
    <dbReference type="eggNOG" id="arCOG04868">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_092286_0_0_2"/>
    <dbReference type="InParanoid" id="Q58259"/>
    <dbReference type="OMA" id="HPFNACL"/>
    <dbReference type="OrthoDB" id="61293at2157"/>
    <dbReference type="PhylomeDB" id="Q58259"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01096">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005865">
        <property type="entry name" value="THM_MeTrfase_su_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04211">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006530">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01148">
        <property type="entry name" value="mtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000147522" description="Tetrahydromethanopterin S-methyltransferase subunit C">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="202"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="265" mass="27804" checksum="07FB20DA394A7FE0" modified="1997-11-01" version="1">MSHGGGGHAAELYPEEQIFAVGIALSLVGCYLANFLSPYGLGMLIGGLLASAACVAGANTVRKVAAYGLGTGVPSIGMVSLGMGTLAAVAGVLIPDYFNLPYLVAPIITLIVSAVIGYIVGRLTVNPVGMKIPIMVRSMTFLSIAGAMALLGFTVAYVGSLEPQKYIDYALNNGMMALAFIAAGMAILHPFNACLGPNESHKRTLTLAVACGFITWFVFSVVKLDIVSIIVSIILWAIVYVKFVKMSFKDACAVLHVPEIPKKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q58264</accession>
    <name>MTRH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tetrahydromethanopterin S-methyltransferase subunit H</fullName>
            <ecNumber evidence="1">2.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mtrH</name>
        <name type="ordered locus">MJ0854</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step. MtrH catalyzes the transfer of the methyl group from methyl-tetrahydromethanopterin to the corrinoid prosthetic group of MtrA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="2.1.1.86"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtrH family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98859" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98859.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64406">
        <property type="entry name" value="F64406"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870368.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58264"/>
    <dbReference type="SMR" id="Q58264"/>
    <dbReference type="STRING" id="243232.MJ_0854"/>
    <dbReference type="DNASU" id="1451742"/>
    <dbReference type="EnsemblBacteria" id="AAB98859">
        <property type="protein sequence ID" value="AAB98859"/>
        <property type="gene ID" value="MJ_0854"/>
    </dbReference>
    <dbReference type="GeneID" id="1451742"/>
    <dbReference type="KEGG" id="mja:MJ_0854"/>
    <dbReference type="eggNOG" id="arCOG04336">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048697_0_0_2"/>
    <dbReference type="InParanoid" id="Q58264"/>
    <dbReference type="OMA" id="YARHKIV"/>
    <dbReference type="OrthoDB" id="86442at2157"/>
    <dbReference type="PhylomeDB" id="Q58264"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01501">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023467">
        <property type="entry name" value="MeTrfase_MtrH/MtxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028342">
        <property type="entry name" value="MtrH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02007">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500206">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004960">
        <property type="entry name" value="MtrH_MtxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01114">
        <property type="entry name" value="mtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147563" description="Tetrahydromethanopterin S-methyltransferase subunit H">
        <location>
            <begin position="1"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="319" mass="34603" checksum="370E20B41F5CDA00" modified="2003-12-15" version="2">MFKFDREQMVVEIAGRKIGGQPGEYPTALAGTIFYARHKIVEDERKGIFDKAAAEDLINKQAEMEDITGNPALVQVFGGTPEALVNYIDFVAEVWDGPMLLDSTSGEARMAAAKRATEAGYAKQCIYNSINVSIDEQEYQVLVESDLEASIVLCFDPMDPTVEGKINVLTNGGKTADKGMLELAEKAGIKYPLIDTAVTPLGNGAGAAVRASFAVKALFGYPVGSGIHNIPSAWDWLREFRKQLREAGEREKAKDIHHVCDVGANLVQVMASGDFVLYGPIDNAYMTFPAVAMVDAIIAEAAKELGIEPIDTHPFKKLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58278</accession>
    <name>Y868_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0868</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0868</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64408">
        <property type="entry name" value="D64408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870383.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58278"/>
    <dbReference type="SMR" id="Q58278"/>
    <dbReference type="STRING" id="243232.MJ_0868"/>
    <dbReference type="EnsemblBacteria" id="AAB98873">
        <property type="protein sequence ID" value="AAB98873"/>
        <property type="gene ID" value="MJ_0868"/>
    </dbReference>
    <dbReference type="GeneID" id="1451757"/>
    <dbReference type="KEGG" id="mja:MJ_0868"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_9_3_2"/>
    <dbReference type="InParanoid" id="Q58278"/>
    <dbReference type="OMA" id="GIHHLYV"/>
    <dbReference type="OrthoDB" id="104239at2157"/>
    <dbReference type="PhylomeDB" id="Q58278"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000107085" description="Uncharacterized protein MJ0868">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="11"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="71"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <sequence length="127" mass="14173" checksum="12B55235ADFE14D3" modified="1996-11-01" version="1">MISKYLVRDVMKKGVVEVTLDTKLSDVIKTMAKYDISSVVVSDGETFWGIITDTDVLKHYNDLDKTAEEIMTTNPITVSPEAPLEKAVEIMAEKGIHHLYVKSPCEDKIVGVLSSKDIIKLFSDLIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58283</accession>
    <name>Y873_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ0873</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0873</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64409">
        <property type="entry name" value="A64409"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58283"/>
    <dbReference type="SMR" id="Q58283"/>
    <dbReference type="STRING" id="243232.MJ_0873"/>
    <dbReference type="EnsemblBacteria" id="AAB98878">
        <property type="protein sequence ID" value="AAB98878"/>
        <property type="gene ID" value="MJ_0873"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0873"/>
    <dbReference type="eggNOG" id="arCOG00198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_11_2"/>
    <dbReference type="InParanoid" id="Q58283"/>
    <dbReference type="OMA" id="KNFFEMS"/>
    <dbReference type="PhylomeDB" id="Q58283"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093222" description="Uncharacterized ABC transporter ATP-binding protein MJ0873">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="290" mass="32550" checksum="01388AC7D21D153D" modified="2000-05-30" version="4">MLKTENLSVGYGNYVVVEGINLEINRGEILCIIGPNGAGKSTLLKTIATYLKPKKGVVYLNGKKIHDLKPKDLAKEMAVVLTERVNPGNMTGFDVVAIGRHPYTDLFGRLTERDKKIIIESARAVNAEYLLEKNFFEMSDGERQKIMIARALAQEPKVLILDEPTSFLDAKHKIELTLLLRKLADEKNLAIVVTLHDIELALRIADKMALIKNHKVIAYGYPENVMKREIVNELYDLKNANYSEVIGYFELKNNPIKNKKIFVICGGGTGANVLRYLVKNRYDVVVGHLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58324</accession>
    <name>VAPC1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease VapC1</fullName>
            <shortName evidence="1">RNase VapC1</shortName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Putative toxin VapC1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">vapC1</name>
        <name type="ordered locus">MJ0914</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>IDENTIFICATION</scope>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Toxic component of a type II toxin-antitoxin (TA) system. An RNase. Its cognate antitoxin is VapB1.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64414">
        <property type="entry name" value="B64414"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58324"/>
    <dbReference type="SMR" id="Q58324"/>
    <dbReference type="STRING" id="243232.MJ_0914"/>
    <dbReference type="EnsemblBacteria" id="AAB98919">
        <property type="protein sequence ID" value="AAB98919"/>
        <property type="gene ID" value="MJ_0914"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0914"/>
    <dbReference type="eggNOG" id="arCOG02219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118482_3_1_2"/>
    <dbReference type="InParanoid" id="Q58324"/>
    <dbReference type="OMA" id="IIGYYTT"/>
    <dbReference type="PhylomeDB" id="Q58324"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00265">
        <property type="entry name" value="VapC_Nob1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022907">
        <property type="entry name" value="VapC_family"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107102" description="Ribonuclease VapC1">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="PINc" evidence="1">
        <location>
            <begin position="18"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00265"/>
        </source>
    </evidence>
    <sequence length="136" mass="16210" checksum="0620F9DFB7831918" modified="1996-11-01" version="1">MKILKKLKKKLEKEESKILVDTSVLIDYFKKRRLEELGGEAISIITAVEFIRGISEHKQEQVLNIFKELFEIVYIDEEIIIPFSKIYRQLKKRGMLIDDADLYIACTAIIKNYPLWTKNKKHFERLKEFGLKIYDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58328</accession>
    <name>TRPC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Indole-3-glycerol phosphate synthase</fullName>
            <shortName>IGPS</shortName>
            <ecNumber>4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpC</name>
        <name type="ordered locus">MJ0918</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpC family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64414">
        <property type="entry name" value="F64414"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58328"/>
    <dbReference type="SMR" id="Q58328"/>
    <dbReference type="STRING" id="243232.MJ_0918"/>
    <dbReference type="EnsemblBacteria" id="AAB98923">
        <property type="protein sequence ID" value="AAB98923"/>
        <property type="gene ID" value="MJ_0918"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0918"/>
    <dbReference type="eggNOG" id="arCOG01088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034247_2_0_2"/>
    <dbReference type="InParanoid" id="Q58328"/>
    <dbReference type="OMA" id="RGPHDLI"/>
    <dbReference type="PhylomeDB" id="Q58328"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="PTHR22854"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154292" description="Indole-3-glycerol phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="266" mass="30027" checksum="0BFD8F7C3C6A5002" modified="1996-11-01" version="1">MKVLDEIVANRKKMVEIEKRKDIIKNLRSFIDELDIDVEKKRKLRLSKAIKKAKEIKNPIITEIKPSSPSKGSIREINLEDVKNIANEMVEGGATALSILTEPKYFNGSYKNLIVAREFDIPILMKDFIVDFYQIDVASEIGANAVLLIVSSLKEDIGEFLDYAKENDLECLVETHSEDEIDIALDAGAKIIGINNRDLKTLKIDLSTTEKLAPLIPKNKIKVGESGIYTKEQLNYVLKFTDAALIGSSIMESENIREKVREFVIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58341</accession>
    <name>THII_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="1">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiI</name>
        <name type="ordered locus">MJ0931</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[sulfur-carrier protein ThiS]-C-terminal Gly-Gly-AMP + AH2 + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = [sulfur-carrier protein ThiS]-C-terminal Gly-NH-CH2-C(O)SH + A + AMP + H(+) + L-cysteinyl-[cysteine desulfurase]</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12908"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:90619"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ThiI family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64416">
        <property type="entry name" value="C64416"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58341"/>
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    <dbReference type="EnsemblBacteria" id="AAB98933">
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        <property type="gene ID" value="MJ_0931"/>
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    <dbReference type="HOGENOM" id="CLU_037952_4_0_2"/>
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    <dbReference type="OMA" id="VIPVHIY"/>
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    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:tRNA adenylyltransferase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA U4 sulfurtransferase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00342">
        <property type="entry name" value="TIGR00342"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000154894" description="Probable tRNA sulfurtransferase">
        <location>
            <begin position="1"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="THUMP" evidence="1">
        <location>
            <begin position="68"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="194"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <sequence length="381" mass="43437" checksum="0A31F1069DA3357B" modified="1996-11-01" version="1">MKKLKVSKMEILVRYGEIGLKSDPIRKNLEEILRKNIIKLLRKYEIDAEVKILHRKLLVKINTKDKEDLALKLLKKVAGIVSYSPVYECPLDINEIVSFAVQIMKKKLKTLNKEKVTFAVKTKRSYKKFPFTSVEVNKKVGEAIVEKLGLEVDLENPDIVLGIEILNDGAYIFTEKYEGIGGLPAGSQGKVLCLISDGIDSPVAAFMMIRRGCRAVLLHLKMSEEALNKVRKIVEVLSDYDTELEFVVYDYKKDIEDIVEKLKSIKKENYTCIFCKRKMLKVAEKYAKYLDCDAIVTGDNLGQVASQTLKNLRVISENINYPILRPLIGLDKNDIVKIAKEIGTYEISTEKEIKCPYLPKHPKTIAKPEEVKKIKEKVKLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q58358</accession>
    <name>Y948_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0948</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0948</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64418">
        <property type="entry name" value="D64418"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58358"/>
    <dbReference type="SMR" id="Q58358"/>
    <dbReference type="STRING" id="243232.MJ_0948"/>
    <dbReference type="EnsemblBacteria" id="AAB98956">
        <property type="protein sequence ID" value="AAB98956"/>
        <property type="gene ID" value="MJ_0948"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0948"/>
    <dbReference type="eggNOG" id="arCOG08286">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123707_0_0_2"/>
    <dbReference type="OMA" id="KKTCMEL"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107117" description="Uncharacterized protein MJ0948">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <sequence length="191" mass="22423" checksum="8D75231083FDB216" modified="1996-11-01" version="1">MIFMKNSTEYPTLVEIKDKKGEMIEKGEAKLRDLNNIRVKLNELRTSNPDDLDTIAQLEEEESHLTSEVLKLDLSIKILEVVEYIIESNIFEDYWKIIEEKIPYEELLNIVVENGLSIKKTCMELYKLANIDDKNILKKIQNLPDDYPKETKEDPNLQNKYLSKIISRISRLKEFKSNLDEIVSDIISNMR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58359</accession>
    <name>Y949_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0949</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0949</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64418">
        <property type="entry name" value="E64418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870463.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58359"/>
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    <dbReference type="STRING" id="243232.MJ_0949"/>
    <dbReference type="EnsemblBacteria" id="AAB98950">
        <property type="protein sequence ID" value="AAB98950"/>
        <property type="gene ID" value="MJ_0949"/>
    </dbReference>
    <dbReference type="GeneID" id="1451846"/>
    <dbReference type="KEGG" id="mja:MJ_0949"/>
    <dbReference type="eggNOG" id="arCOG04842">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145818_0_0_2"/>
    <dbReference type="InParanoid" id="Q58359"/>
    <dbReference type="OMA" id="YGGMKIR"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007154">
        <property type="entry name" value="DUF356"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04009">
        <property type="entry name" value="DUF356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006606">
        <property type="entry name" value="UCP006606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107118" description="Uncharacterized protein MJ0949">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <sequence length="123" mass="13959" checksum="DA8F66F87739E34B" modified="1996-11-01" version="1">MTILLIRGDSYEKLKNALADVDRHAELTIIGKPKIIVPEAADEILSHILGEVKKPCKTACLAKIAEKAPKAIDRIRKIHPPAHIVVISERYGDIYYKLLDDFPKLPVLKGYYKSKKKDKKKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58406</accession>
    <name>SYH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Histidine--tRNA ligase</fullName>
            <ecNumber>6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Histidyl-tRNA synthetase</fullName>
            <shortName>HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisS</name>
        <name type="ordered locus">MJ1000</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-histidine + tRNA(His) = AMP + diphosphate + H(+) + L-histidyl-tRNA(His)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64424">
        <property type="entry name" value="G64424"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58406"/>
    <dbReference type="SMR" id="Q58406"/>
    <dbReference type="STRING" id="243232.MJ_1000"/>
    <dbReference type="EnsemblBacteria" id="AAB99003">
        <property type="protein sequence ID" value="AAB99003"/>
        <property type="gene ID" value="MJ_1000"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1000"/>
    <dbReference type="eggNOG" id="arCOG00404">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_3_1_2"/>
    <dbReference type="InParanoid" id="Q58406"/>
    <dbReference type="OMA" id="CDFDFIG"/>
    <dbReference type="PhylomeDB" id="Q58406"/>
    <dbReference type="BRENDA" id="6.1.1.21">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="PTHR43707"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00443">
        <property type="entry name" value="hisZ_biosyn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136312" description="Histidine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="416" mass="48322" checksum="D7541F81CEAA8679" modified="1997-11-01" version="1">MIVMFQKPRGTRDFLPEEMKKRRFVENKLREVFERYGYKEILTPTFESFELIAKKTGEEIRKQLYVFKDHGGREMALRPEMTSPVVRFYLNELKNLQKPLRLYYFANCFRYERPQAGRFREFWQMGCELIGCKEPLADAEVLNLAMDGLINIGLDFDVHIGHLGVLKGVLEKFNVSEEEEVKIRRLIDKEDYDNLKIYLTQILGEEKKELIFEILKFKGSREVLDELKEILKDFPKSMEAINNLEEILEFVIHDKYTINLGIARGLDYYTGMVFEIYGKKGAKQICGGGRYDNLIETFGGEPTPAVGFAYGFDRIMMNIDDLDIEEESILIIPVKKDKELIKKSLIIADKLRKAGKIVELEIMGRKLRKALDYANSRGFKKVIIVGEKELNEGKVTVKDMITGEQKLIGIDELTNF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58515</accession>
    <name>Y1115_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1115</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1115</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BtpA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64439">
        <property type="entry name" value="B64439"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58515"/>
    <dbReference type="STRING" id="243232.MJ_1115"/>
    <dbReference type="DNASU" id="1452011"/>
    <dbReference type="EnsemblBacteria" id="AAB99116">
        <property type="protein sequence ID" value="AAB99116"/>
        <property type="gene ID" value="MJ_1115"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1115"/>
    <dbReference type="eggNOG" id="arCOG01982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075239_1_0_2"/>
    <dbReference type="InParanoid" id="Q58515"/>
    <dbReference type="OMA" id="ENFFDAP"/>
    <dbReference type="PhylomeDB" id="Q58515"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005137">
        <property type="entry name" value="BtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381">
        <property type="entry name" value="PTHR21381"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03437">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005956">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00259">
        <property type="entry name" value="thylakoid_BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159331" description="Uncharacterized protein MJ1115">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="261" mass="28939" checksum="D831E2F79BD69517" modified="1996-11-01" version="1">MIPMFKKKPLIGMVHLKPLPGSYHYNDNFDDIVDFAIKEAKKLEEAGFDAVMIENFGDAPFKKEADKITIASMAVIAKAIKEEVSLPLGINILRNDAIGAYSIAYVVKADFIRVNVLSGVAFTDQGIIEGKAYELAKLKKLLPSKIKVFADVHVKHAYHFIDFESSLLDTVERGLADAVIISGKRTGKEVDIEKLKLAKELVDVPVIVGSGTNYNNLRILWSYADGFIIGTWIKKDGKANNEIDIDRAKKIVNLANKLKMC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58520</accession>
    <name>Y1120_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ1120</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1120</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64439">
        <property type="entry name" value="G64439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870631.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58520"/>
    <dbReference type="SMR" id="Q58520"/>
    <dbReference type="STRING" id="243232.MJ_1120"/>
    <dbReference type="EnsemblBacteria" id="AAB99121">
        <property type="protein sequence ID" value="AAB99121"/>
        <property type="gene ID" value="MJ_1120"/>
    </dbReference>
    <dbReference type="GeneID" id="1452016"/>
    <dbReference type="KEGG" id="mja:MJ_1120"/>
    <dbReference type="eggNOG" id="arCOG00225">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_992530_0_0_2"/>
    <dbReference type="OMA" id="AHRIVWK"/>
    <dbReference type="OrthoDB" id="54698at2157"/>
    <dbReference type="PhylomeDB" id="Q58520"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="Tscrpt_reg_HTH_LysR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000105832" description="Uncharacterized HTH-type transcriptional regulator MJ1120">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="HTH lysR-type" evidence="1">
        <location>
            <begin position="14"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="279" mass="32209" checksum="2F15753B5BAB4952" modified="1996-11-01" version="1">MKVDLTIEYRGKLITPNQIKLLIALHKTKSQNEAAKLLNIKPSSFNIQLKRLENKLGVKLYYSSPNGTVLTDAGLEILETYNSYSKRLKNHFFTVSGFVSGEIAKILFGNPIITSFDNALKLLKMGFVDVLGVDDSYWIYRLGDERFLKSEIGSCDFNIFLIAYDNFVMVSKKEFNYKNLVGIRFSSQRIVYNILKKEGIKFKVKVRVKNPFRAIELVNEGYSLFLNESLLRYIDGDFNVIYPNFYEKTVHAINFISFGKDIEIDKKEIKKIKKLGFKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q58525</accession>
    <name>Y1125_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1125</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1125</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64440">
        <property type="entry name" value="D64440"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58525"/>
    <dbReference type="STRING" id="243232.MJ_1125"/>
    <dbReference type="EnsemblBacteria" id="AAB99127">
        <property type="protein sequence ID" value="AAB99127"/>
        <property type="gene ID" value="MJ_1125"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1125"/>
    <dbReference type="eggNOG" id="arCOG03445">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2930225_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107179" description="Uncharacterized protein MJ1125">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <sequence length="60" mass="6905" checksum="7393670630AC1E63" modified="1996-11-01" version="1">MMVKNTIDVKEILRELDTTRIKDYPLMSGKEILLRTNFKGCCGMPSQISQLNLKEQLESC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58536</accession>
    <name>ELP3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA uridine(34) acetyltransferase</fullName>
            <ecNumber evidence="2">2.3.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Elongator complex protein 3 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1136</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">tRNA uridine(34) acetyltransferase, which mediates formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. The proposed mechanism is the following: (i) recruits S-adenosyl-L-methionine and cleaves it to generate a 5'-deoxyadenosine radical (5'-dA) in the radical S-adenosyl-L-methionine (rSAM) region, (ii) hydrolyzes acetyl-CoA in the N-acetyltransferase domain and (iii) an acetyl radical is formed by the products of the two domains and (iv) is transferred onto the C5 position of uridine(34) in the bound tRNA molecule. Does not show protein lysine acetyltransferase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>acetyl-CoA + H2O + S-adenosyl-L-methionine + uridine(34) in tRNA = 5'-deoxyadenosine + carboxymethyluridine(34) in tRNA + CoA + 2 H(+) + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ELP3 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64441">
        <property type="entry name" value="G64441"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58536"/>
    <dbReference type="SMR" id="Q58536"/>
    <dbReference type="STRING" id="243232.MJ_1136"/>
    <dbReference type="DNASU" id="1452032"/>
    <dbReference type="EnsemblBacteria" id="AAB99138">
        <property type="protein sequence ID" value="AAB99138"/>
        <property type="gene ID" value="MJ_1136"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1136"/>
    <dbReference type="eggNOG" id="arCOG01361">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025983_2_1_2"/>
    <dbReference type="InParanoid" id="Q58536"/>
    <dbReference type="OMA" id="TFETRPD"/>
    <dbReference type="PhylomeDB" id="Q58536"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="PTHR11135:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000107183" description="tRNA uridine(34) acetyltransferase">
        <location>
            <begin position="1"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="79"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="401"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="region of interest" description="Radical S-adenosyl-L-methionine (rSAM)" evidence="2">
        <location>
            <begin position="76"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="467"/>
            <end position="470"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="491"/>
            <end position="493"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A1C7D1B7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="D5VRB9"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q02908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="541" mass="62443" checksum="31794B06F11E0BFD" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58585</accession>
    <name>Y1185_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1185</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1185</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AOR/FOR family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64447">
        <property type="entry name" value="H64447"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870698.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58585"/>
    <dbReference type="SMR" id="Q58585"/>
    <dbReference type="STRING" id="243232.MJ_1185"/>
    <dbReference type="EnsemblBacteria" id="AAB99186">
        <property type="protein sequence ID" value="AAB99186"/>
        <property type="gene ID" value="MJ_1185"/>
    </dbReference>
    <dbReference type="GeneID" id="1452083"/>
    <dbReference type="KEGG" id="mja:MJ_1185"/>
    <dbReference type="eggNOG" id="arCOG05072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_440510_0_0_2"/>
    <dbReference type="InParanoid" id="Q58585"/>
    <dbReference type="OMA" id="CRFHRGW"/>
    <dbReference type="OrthoDB" id="5023at2157"/>
    <dbReference type="PhylomeDB" id="Q58585"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="SSF48310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="SSF56228"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107208" description="Uncharacterized protein MJ1185">
        <location>
            <begin position="1"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="622" mass="72499" checksum="6DD910C20DC47678" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58595</accession>
    <name>LEU1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2-isopropylmalate synthase</fullName>
            <ecNumber>2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuA</name>
        <name type="ordered locus">MJ1195</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Biochemistry" volume="37" first="10108" last="10117">
            <title>Alpha-keto acid chain elongation reactions involved in the biosynthesis of coenzyme B (7-mercaptoheptanoyl threonine phosphate) in methanogenic Archaea.</title>
            <authorList>
                <person name="Howell D.M."/>
                <person name="Harich K."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="9665716"/>
            <dbReference type="DOI" id="10.1021/bi980662p"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64449">
        <property type="entry name" value="B64449"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870707.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58595"/>
    <dbReference type="SMR" id="Q58595"/>
    <dbReference type="STRING" id="243232.MJ_1195"/>
    <dbReference type="EnsemblBacteria" id="AAB99199">
        <property type="protein sequence ID" value="AAB99199"/>
        <property type="gene ID" value="MJ_1195"/>
    </dbReference>
    <dbReference type="GeneID" id="1452090"/>
    <dbReference type="KEGG" id="mja:MJ_1195"/>
    <dbReference type="eggNOG" id="arCOG02092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022158_0_1_2"/>
    <dbReference type="InParanoid" id="Q58595"/>
    <dbReference type="OMA" id="NTMRMLV"/>
    <dbReference type="OrthoDB" id="10632at2157"/>
    <dbReference type="PhylomeDB" id="Q58595"/>
    <dbReference type="BRENDA" id="2.3.3.13">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00070"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="SSF110921"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000140411" description="2-isopropylmalate synthase">
        <location>
            <begin position="1"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="1">
        <location>
            <begin position="24"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="9665716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="518" mass="56620" checksum="604AB61B41E607A4" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58610</accession>
    <name>Y1213_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1213</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1213</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0123 and A.aeolicus AA15.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64451">
        <property type="entry name" value="D64451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870725.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4QHF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="4QHG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="4QHH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="4QHI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=1-110"/>
    </dbReference>
    <dbReference type="PDB" id="4QHJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A/B=1-110"/>
    </dbReference>
    <dbReference type="PDBsum" id="4QHF"/>
    <dbReference type="PDBsum" id="4QHG"/>
    <dbReference type="PDBsum" id="4QHH"/>
    <dbReference type="PDBsum" id="4QHI"/>
    <dbReference type="PDBsum" id="4QHJ"/>
    <dbReference type="AlphaFoldDB" id="Q58610"/>
    <dbReference type="SMR" id="Q58610"/>
    <dbReference type="STRING" id="243232.MJ_1213"/>
    <dbReference type="EnsemblBacteria" id="AAB99223">
        <property type="protein sequence ID" value="AAB99223"/>
        <property type="gene ID" value="MJ_1213"/>
    </dbReference>
    <dbReference type="GeneID" id="1452109"/>
    <dbReference type="KEGG" id="mja:MJ_1213"/>
    <dbReference type="eggNOG" id="arCOG09626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2067800_0_0_2"/>
    <dbReference type="InParanoid" id="Q58610"/>
    <dbReference type="OMA" id="KYHINEF"/>
    <dbReference type="OrthoDB" id="138037at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008756">
        <property type="entry name" value="Peptidase_M56"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05569">
        <property type="entry name" value="Peptidase_M56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107216" description="Uncharacterized protein MJ1213">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="4"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="28"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="38"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="4QHG"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4QHJ"/>
        </source>
    </evidence>
    <sequence length="110" mass="13080" checksum="D94E9F492B28F24B" modified="1997-11-01" version="1">MKDRKILNEILSNTINELNLNDKKANIKIKIKPLKRKIASISLTNKTIYINKNILPYLSDEEIRFILAHELLHLKYGKYHINEFEEELLFLFPNKEAILINLINKLHQKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58638</accession>
    <name>Y1241_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1241</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1241</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64454">
        <property type="entry name" value="H64454"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58638"/>
    <dbReference type="STRING" id="243232.MJ_1241"/>
    <dbReference type="EnsemblBacteria" id="AAB99246">
        <property type="protein sequence ID" value="AAB99246"/>
        <property type="gene ID" value="MJ_1241"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1241"/>
    <dbReference type="eggNOG" id="arCOG01040">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2730398_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107234" description="Uncharacterized protein MJ1241">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <sequence length="71" mass="8128" checksum="9D74BEE228F13AB4" modified="1996-11-01" version="1">MVLKMEIKWYVKRGFEDNLIDALNTYGSACVLGLAGMGKTTIARYIYTKLRREGVKVVYLTSDEESKTIKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58641</accession>
    <name>Y1244_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1244</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1244</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1245 and M.thermoautotrophicum MTH1110.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64455">
        <property type="entry name" value="C64455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870756.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58641"/>
    <dbReference type="SMR" id="Q58641"/>
    <dbReference type="STRING" id="243232.MJ_1244"/>
    <dbReference type="EnsemblBacteria" id="AAB99249">
        <property type="protein sequence ID" value="AAB99249"/>
        <property type="gene ID" value="MJ_1244"/>
    </dbReference>
    <dbReference type="GeneID" id="1452140"/>
    <dbReference type="KEGG" id="mja:MJ_1244"/>
    <dbReference type="eggNOG" id="arCOG03424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145179_0_0_2"/>
    <dbReference type="OMA" id="KYTIIEI"/>
    <dbReference type="OrthoDB" id="103140at2157"/>
    <dbReference type="PhylomeDB" id="Q58641"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024184">
        <property type="entry name" value="UCP005637"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019296">
        <property type="entry name" value="Unchr_N-regulatory-PII-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10126">
        <property type="entry name" value="Nit_Regul_Hom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005637">
        <property type="entry name" value="UCP005637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107235" description="Uncharacterized protein MJ1244">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="108" mass="12029" checksum="098F29966DB174A0" modified="1996-11-01" version="1">MKILLYLFVESENVGKAINALSEGGITGFFLYDYKGMSPQDWQGFLLDEDPEMAIKAVSDLAQNAVLIGTIVSENKLMEIEKLIDEKLADCKYTIIEIPIEGIIVNMP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58707</accession>
    <name>Y1311_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1311</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1311</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1614 and MJ0008.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64463">
        <property type="entry name" value="F64463"/>
    </dbReference>
    <dbReference type="PDB" id="3WQO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.64 A"/>
        <property type="chains" value="A/B=1-293"/>
    </dbReference>
    <dbReference type="PDBsum" id="3WQO"/>
    <dbReference type="AlphaFoldDB" id="Q58707"/>
    <dbReference type="SMR" id="Q58707"/>
    <dbReference type="STRING" id="243232.MJ_1311"/>
    <dbReference type="DNASU" id="1452213"/>
    <dbReference type="EnsemblBacteria" id="AAB99318">
        <property type="protein sequence ID" value="AAB99318"/>
        <property type="gene ID" value="MJ_1311"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1311"/>
    <dbReference type="eggNOG" id="arCOG01895">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050006_7_2_2"/>
    <dbReference type="InParanoid" id="Q58707"/>
    <dbReference type="OMA" id="MDEGLHY"/>
    <dbReference type="PhylomeDB" id="Q58707"/>
    <dbReference type="BRENDA" id="5.1.3.31">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107269" description="Uncharacterized protein MJ1311">
        <location>
            <begin position="1"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="18"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="23"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="26"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="31"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="44"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="52"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="74"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="114"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="131"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="155"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="172"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="187"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="193"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="202"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="205"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="216"/>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="225"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="236"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="241"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3WQO"/>
        </source>
    </evidence>
    <sequence length="293" mass="33260" checksum="0B30E5A286CE38F3" modified="1997-11-01" version="1">MKRKTKLDKKSKWVLDMKFGVSSLVFLPESLTSSMEKIAEHNFDAWEIVCEGTHYLSPKNIKYLMELRDRYEVEIVVHAPFSDLNPASMNERVRKLTVECIRDAIEGAFELDSEVVVVHPGYIPELWSNYVSEILDNNFSTLSEIVEIAEDYGIKIGLENMPNFRGVLGITPESLLEIVKDIDSKNLGITFDIGHANTAGNPAEFVEKLQNIGIGIIHVHAHDNNGYDDEHLKIGEGNINFIEVLEKLKEIGYDGVISIENKNIRDAVKSKEILKEYLEIVNEKVAEKEKIEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58708</accession>
    <name>Y1312_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0026 protein MJ1312</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1312</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0026 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64463">
        <property type="entry name" value="G64463"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870829.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58708"/>
    <dbReference type="SMR" id="Q58708"/>
    <dbReference type="STRING" id="243232.MJ_1312"/>
    <dbReference type="DNASU" id="1452214"/>
    <dbReference type="EnsemblBacteria" id="AAB99319">
        <property type="protein sequence ID" value="AAB99319"/>
        <property type="gene ID" value="MJ_1312"/>
    </dbReference>
    <dbReference type="GeneID" id="1452214"/>
    <dbReference type="KEGG" id="mja:MJ_1312"/>
    <dbReference type="eggNOG" id="arCOG00953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058377_0_0_2"/>
    <dbReference type="InParanoid" id="Q58708"/>
    <dbReference type="OMA" id="CIYCELG"/>
    <dbReference type="OrthoDB" id="73459at2157"/>
    <dbReference type="PhylomeDB" id="Q58708"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040084">
        <property type="entry name" value="GTPase_Obg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01083">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000217863" description="UPF0026 protein MJ1312">
        <location>
            <begin position="1"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="11"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="321" mass="37131" checksum="0035C4E648DA607F" modified="1996-11-01" version="1">MTIAFGPVPSRRLGKSLGINSIPCKFCSYDCVYCQVGRTINKTIERREFYSPEDIFKSVEERIGKLNNEKIDYLTFVADGEPTLDINLSKEVEMLRDFDIPIAIITNSSLIWREDVRNDILNFDLVSFKVDSVDEKIWREINRPHKDLVLDKILEGMIAFRDNYKGELITETMILGSIKYTEESIIKTAEFLKELNPNKCYLNTPIRPPSEKYIKPPKIEVITKILAIFNEIIGKNKIKLLGKFEGNEFIFSENVEEDILAITSVHPMREEVIKELLNKSNISFDIINKMVNEGKLIKLEYDGKVFYMKNIKSRDKNVSNP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58746</accession>
    <accession>Q4U2V1</accession>
    <name>AGLUS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Archaeal glutamate synthase [NADPH]</fullName>
            <ecNumber>1.4.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Archaeal NADPH-GOGAT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1351</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2005-04" db="EMBL/GenBank/DDBJ databases">
            <title>Putative FMN-binding domain of glutamate synthase.</title>
            <authorList>
                <person name="Dincturk H.B."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2 L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + L-glutamine + NADPH</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13"/>
    <dbReference type="EMBL" id="DQ018115">
        <property type="protein sequence ID" value="AAY33887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64468">
        <property type="entry name" value="F64468"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870869.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58746"/>
    <dbReference type="SMR" id="Q58746"/>
    <dbReference type="STRING" id="243232.MJ_1351"/>
    <dbReference type="EnsemblBacteria" id="AAB99362">
        <property type="protein sequence ID" value="AAB99362"/>
        <property type="gene ID" value="MJ_1351"/>
    </dbReference>
    <dbReference type="GeneID" id="1452254"/>
    <dbReference type="KEGG" id="mja:MJ_1351"/>
    <dbReference type="eggNOG" id="arCOG00619">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023342_1_1_2"/>
    <dbReference type="InParanoid" id="Q58746"/>
    <dbReference type="OMA" id="CAWGIAT"/>
    <dbReference type="OrthoDB" id="14505at2157"/>
    <dbReference type="PhylomeDB" id="Q58746"/>
    <dbReference type="BRENDA" id="1.4.1.13">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043578">
        <property type="entry name" value="GltB_archl_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500061">
        <property type="entry name" value="GOGAT_lg2_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000170804" description="Archaeal glutamate synthase [NADPH]">
        <location>
            <begin position="1"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="10"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="510" mass="55454" checksum="4EE38CB9351D1CFA" modified="1996-11-01" version="1">MIPSYVPPKYKVEVDPNRCMLCERCTIECSWGVYRREGDRIISYSNRCGACHRCVVMCPRDAITIKENAISWRSHPLWDVDARVDIYNQAKTGCILLSGMGNAKEHPIYFDKIVLDACQVTNPSIDPLREPMELRTYIGKKPKQLEFEFVEEEIDGKKIKKAKLKTKIAPNLKLDTPIMIAHMSYGALSLNAHLSFAKAVKECGTFMGTGEGGLPKALYPYADHIITQVASGRFGVNEEYLMKGSAIEIKIGQGAKPGIGGHLPGEKVTAEISATRMIPEGSDAISPAPHHDIYSIEDLAQLVRSLKEATRWKKPVFVKIAAVHNAPAIAVGIATSDADAVVIDGYKGGTGAAPKVFRDHVGIPIEMAIAAVDQRLREEGLRNEISIIASGGIRCSADVFKAIALGADAVYIGTAAMVALGCRVCGRCYTGLCAWGIATQRPELVKRLDPEVGARRVANLIKAWTHEIKELLGAAGINSIESLRGNRDRLRGVGLNEKELEVLGIKAAGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58751</accession>
    <name>Y1356_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1356</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1356</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1461.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64469">
        <property type="entry name" value="C64469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870873.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58751"/>
    <dbReference type="SMR" id="Q58751"/>
    <dbReference type="STRING" id="243232.MJ_1356"/>
    <dbReference type="EnsemblBacteria" id="AAB99367">
        <property type="protein sequence ID" value="AAB99367"/>
        <property type="gene ID" value="MJ_1356"/>
    </dbReference>
    <dbReference type="GeneID" id="1452258"/>
    <dbReference type="KEGG" id="mja:MJ_1356"/>
    <dbReference type="eggNOG" id="arCOG09681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431639_0_0_2"/>
    <dbReference type="OMA" id="YIGINQN"/>
    <dbReference type="OrthoDB" id="82541at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019825">
        <property type="term" value="F:oxygen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044398">
        <property type="entry name" value="Globin-sensor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012292">
        <property type="entry name" value="Globin/Proto"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11563">
        <property type="entry name" value="Protoglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107295" description="Uncharacterized protein MJ1356">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="189" mass="22350" checksum="A3812C6308D6390E" modified="1996-11-01" version="1">MNVTFDGIYNEIIENMHHFASEEKDFSKLTQYKDLISKTIDEVVEEVFNDIFSYEKTKTLFDESKRKELEEDFKNWIKGLFEISNDSDLNEFYKEIVKRGIKYVEKDFLPEYLTAIIIKIEDRLKNKLKEELKEDAQEIVDILDDLLKRVILLNVAAYMNFESKVLDYIGINQNLKKNAVKLGIKKMGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58779</accession>
    <name>Y1384_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative nitroreductase MJ1384</fullName>
            <ecNumber>1.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1384</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nitroreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64472">
        <property type="entry name" value="G64472"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58779"/>
    <dbReference type="SMR" id="Q58779"/>
    <dbReference type="STRING" id="243232.MJ_1384"/>
    <dbReference type="EnsemblBacteria" id="AAB99394">
        <property type="protein sequence ID" value="AAB99394"/>
        <property type="gene ID" value="MJ_1384"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1384"/>
    <dbReference type="eggNOG" id="arCOG00288">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059362_3_1_2"/>
    <dbReference type="InParanoid" id="Q58779"/>
    <dbReference type="OMA" id="CAIAAYD"/>
    <dbReference type="PhylomeDB" id="Q58779"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020051">
        <property type="entry name" value="SagB-type_dehydrogenase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="SSF55469"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03605">
        <property type="entry name" value="antibiot_sagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000072720" description="Putative nitroreductase MJ1384">
        <location>
            <begin position="1"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="198" mass="22403" checksum="7737D2E2DED8E8A6" modified="1996-11-01" version="1">MGDTMEIQLPDIEEIKLEDVLIKRRSVREYCSSPLTLRELSHILFAAYGVTDERGFKTVPSAGATYPLEIYVNVRDVVGVEEGVYKYIPERHSIVRILDEEVGHELALAALKQMFIAIAPIVLIIAANYERTTRVYGDRGFRYVHMEVGHVAQNVYLMATSLGLGTVSVGAFYDNEIREILKIKEYPLLLMPVGRKIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58810</accession>
    <name>SYW_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="1">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpS</name>
        <name type="ordered locus">MJ1415</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + H(+) + L-tryptophanyl-tRNA(Trp)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64476">
        <property type="entry name" value="F64476"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58810"/>
    <dbReference type="SMR" id="Q58810"/>
    <dbReference type="STRING" id="243232.MJ_1415"/>
    <dbReference type="PRIDE" id="Q58810"/>
    <dbReference type="EnsemblBacteria" id="AAB99425">
        <property type="protein sequence ID" value="AAB99425"/>
        <property type="gene ID" value="MJ_1415"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1415"/>
    <dbReference type="eggNOG" id="arCOG01887">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032621_3_0_2"/>
    <dbReference type="InParanoid" id="Q58810"/>
    <dbReference type="OMA" id="SIYHRFM"/>
    <dbReference type="PhylomeDB" id="Q58810"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000136723" description="Tryptophan--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="75"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="255"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <sequence length="370" mass="42661" checksum="E6C71107CF82B59D" modified="1997-11-01" version="1">MKLMELTPWETPAVIDYKKTMEQFGVKPIVDVLGDLKEEHHFFRRNIILGHRDFERIVDAIKNNKEFAVVSGMMPSGKMHFGHKMVVDLLKFYQKYTDNINIPIADLEAYWARNMSFETTKELALNEYITNYIALGLDPEKINVYLQSKYQKVKDLALILSKRTNWSEMKAIYGFKGETNIGHVFAPIVQVADILHPQLDENLSPEPKPVVVPVGIDQDPHIRLTRDIANRAKEFKFIPPSSTYHRFMTGLLGGKMSSSKPETAIFLTDDEKTVKKKIFSAKTGGRETLEEHKKYGGVPEECVVYELFLYHLILDDKELAEIYQKCRSGELTCGKCKKMAYERVVEFLKDLKEKREQAKEIAVKILEGKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="148" xmlns="http://uniprot.org/uniprot">
    <accession>Q58815</accession>
    <name>GLMS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Glutamine--fructose-6-phosphate aminotransferase [isomerizing]</fullName>
            <ecNumber>2.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>GFAT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Glucosamine-6-phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Hexosephosphate aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>L-glutamine--D-fructose-6-phosphate amidotransferase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mja gf6p intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">glmS</name>
        <name type="ordered locus">MJ1420</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">In the C-terminal section; belongs to the SIS family. GFAT subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="7">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99430" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.6.1.16"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99430.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64477">
        <property type="entry name" value="C64477"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58815"/>
    <dbReference type="SMR" id="Q58815"/>
    <dbReference type="STRING" id="243232.MJ_1420"/>
    <dbReference type="EnsemblBacteria" id="AAB99430">
        <property type="protein sequence ID" value="AAB99430"/>
        <property type="gene ID" value="MJ_1420"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1420"/>
    <dbReference type="eggNOG" id="arCOG00057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_285704_0_0_2"/>
    <dbReference type="InParanoid" id="Q58815"/>
    <dbReference type="OMA" id="NSRWATH"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GlmS_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="PTHR10937:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SSF53697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000010848" description="Glutamine--fructose-6-phosphate aminotransferase [isomerizing], 1st part" evidence="2">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000010849" description="Mja gf6p intein" evidence="2">
        <location>
            <begin position="72"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000010850" description="Glutamine--fructose-6-phosphate aminotransferase [isomerizing], 2nd part" evidence="2">
        <location>
            <begin position="571"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2; first part" evidence="5">
        <location>
            <begin position="2"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="3">
        <location>
            <begin position="198"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="4">
        <location>
            <begin position="278"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2; second part" evidence="5">
        <location>
            <begin position="571"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="domain" description="SIS 1" evidence="6">
        <location>
            <begin position="786"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="domain" description="SIS 2" evidence="6">
        <location>
            <begin position="948"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="active site" description="For Fru-6P isomerization activity" evidence="1">
        <location>
            <position position="1094"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <sequence length="1099" mass="125501" checksum="A4CFE30CB3894F16" modified="2007-01-23" version="3"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58825</accession>
    <name>HIS3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="1">PRA-CH</shortName>
            <ecNumber evidence="1">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisI</name>
        <name type="ordered locus">MJ1430</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRA-CH family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64478">
        <property type="entry name" value="E64478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870948.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58825"/>
    <dbReference type="SMR" id="Q58825"/>
    <dbReference type="STRING" id="243232.MJ_1430"/>
    <dbReference type="DNASU" id="1452334"/>
    <dbReference type="EnsemblBacteria" id="AAB99440">
        <property type="protein sequence ID" value="AAB99440"/>
        <property type="gene ID" value="MJ_1430"/>
    </dbReference>
    <dbReference type="GeneID" id="1452334"/>
    <dbReference type="KEGG" id="mja:MJ_1430"/>
    <dbReference type="eggNOG" id="arCOG02676">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_5_3_2"/>
    <dbReference type="InParanoid" id="Q58825"/>
    <dbReference type="OMA" id="YSCFHYK"/>
    <dbReference type="OrthoDB" id="101275at2157"/>
    <dbReference type="PhylomeDB" id="Q58825"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="SSF141734"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000136507" description="Phosphoribosyl-AMP cyclohydrolase">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <sequence length="127" mass="14926" checksum="DEDB2D6D46431B2B" modified="1996-11-01" version="1">MDVEDTVKKLNLKFRNIEGERLILAITCDENKNVLMVAFMNEEALKKTLETGYMHYYSTSRKKLWRKGEESGNVQKLIKFYRDCDGDALLFIVEQKGVACHEGYYSCFHYKIEDGELKITGEYYSKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58828</accession>
    <name>Y1433_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1433</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1433</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64478">
        <property type="entry name" value="H64478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870951.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58828"/>
    <dbReference type="SMR" id="Q58828"/>
    <dbReference type="STRING" id="243232.MJ_1433"/>
    <dbReference type="EnsemblBacteria" id="AAB99443">
        <property type="protein sequence ID" value="AAB99443"/>
        <property type="gene ID" value="MJ_1433"/>
    </dbReference>
    <dbReference type="GeneID" id="1452337"/>
    <dbReference type="KEGG" id="mja:MJ_1433"/>
    <dbReference type="eggNOG" id="arCOG03165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087537_0_0_2"/>
    <dbReference type="OMA" id="SEWLDYM"/>
    <dbReference type="OrthoDB" id="94554at2157"/>
    <dbReference type="PhylomeDB" id="Q58828"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107325" description="Uncharacterized protein MJ1433">
        <location>
            <begin position="1"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="247" mass="29480" checksum="1C88A2B8EB4B304D" modified="1996-11-01" version="1">MNDKRYMLIIAICLIFLSILVYSIHFLIFGKVDYILSYFLLHLAFVPIEVLLVSLIIEKILDYREKKKILEKLNMVVGSFFNSVGEELLKIILEGDVGNIRDYLKISDEWNDKTYEETKKLLMNYDCNIDIEKIDLYKLKNLLERNKEFLLRLMENPLLLEHESFTELLLAVFHLADELHRREDLSNLPKSDLDHLKNDIIRVYKLLIIQWLNYLMHLKDNYPYLYSLCLRANPFDNKSIIIEEDDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58851</accession>
    <name>HISX_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Histidinol dehydrogenase</fullName>
            <shortName>HDH</shortName>
            <ecNumber>1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hisD</name>
        <name type="ordered locus">MJ1456</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>H2O + L-histidinol + 2 NAD(+) = 3 H(+) + L-histidine + 2 NADH</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64481">
        <property type="entry name" value="G64481"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58851"/>
    <dbReference type="SMR" id="Q58851"/>
    <dbReference type="STRING" id="243232.MJ_1456"/>
    <dbReference type="EnsemblBacteria" id="AAB99465">
        <property type="protein sequence ID" value="AAB99465"/>
        <property type="gene ID" value="MJ_1456"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1456"/>
    <dbReference type="eggNOG" id="arCOG04352">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_3_0_2"/>
    <dbReference type="InParanoid" id="Q58851"/>
    <dbReference type="OMA" id="MVTGPGN"/>
    <dbReference type="PhylomeDB" id="Q58851"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="PTHR21256"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="SSF53720"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000135894" description="Histidinol dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="328"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="420"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="429" mass="47433" checksum="5E4DF4B17699EDB8" modified="1996-11-01" version="1">MVTGMIIKKIKELTKEEEEKIINRNKANFEEILPTVMEILKDVKEKGDEALKYYTKKFDGVEIEDFKVTDEEIEEAYNSVDYKVVEAIERAKENIYFFHKKQMEQIKDLNVENNGIILGQVVRAIEKVGCYVPGGRAFYPSTVLMTTIPAKVAGCEEIYITSPPTKDGKGNPATLIAGDIVGVSAIYKVGGVQAIGALAYGTETIPKVDIIVGPGNIYVTTAKKMVYGEVAIDFLAGPSEVLIIADETANAEFVALDFIAQAEHDPNASCVITTTSEKKAEEIKNKIFEEIEKAERKEIILKALENSAILIGDLEECIEFSNKYAPEHLEILTKNPEEVLNKIKHAGSVFLGEYSPVPVGDYASGTNHVLPTSQFARMSSGLNVETFLKKITYQKLDKESLKNIADIVITLAEAEGLFGHAEAVRRRLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58852</accession>
    <name>Y1457_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1457</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1457</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64481">
        <property type="entry name" value="H64481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870977.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58852"/>
    <dbReference type="SMR" id="Q58852"/>
    <dbReference type="STRING" id="243232.MJ_1457"/>
    <dbReference type="EnsemblBacteria" id="AAB99470">
        <property type="protein sequence ID" value="AAB99470"/>
        <property type="gene ID" value="MJ_1457"/>
    </dbReference>
    <dbReference type="GeneID" id="1452361"/>
    <dbReference type="KEGG" id="mja:MJ_1457"/>
    <dbReference type="eggNOG" id="arCOG11086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2152648_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107345" description="Uncharacterized protein MJ1457">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="135" mass="14508" checksum="E69A4F18B630A92D" modified="1996-11-01" version="1">MMVMTALTLLILFFGLAFILVGFGLILGYLIFSRKNKTVNGTANRTVVVERDSSNLLGTAAAVAGGVIAGELITDAIEDVINNNENNEADGYNSEGIETTIEDTIEEIDKGVDNVIEDITDEIDNITNDIGDSFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58854</accession>
    <name>ADEC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Adenine deaminase</fullName>
            <shortName evidence="1">Adenase</shortName>
            <shortName evidence="1">Adenine aminase</shortName>
            <ecNumber evidence="1">3.5.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ade</name>
        <name type="ordered locus">MJ1459</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenine + H(+) + H2O = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64482">
        <property type="entry name" value="B64482"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58854"/>
    <dbReference type="SMR" id="Q58854"/>
    <dbReference type="STRING" id="243232.MJ_1459"/>
    <dbReference type="EnsemblBacteria" id="AAB99467">
        <property type="protein sequence ID" value="AAB99467"/>
        <property type="gene ID" value="MJ_1459"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1459"/>
    <dbReference type="eggNOG" id="arCOG00693">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027935_0_0_2"/>
    <dbReference type="InParanoid" id="Q58854"/>
    <dbReference type="OMA" id="TDHECFT"/>
    <dbReference type="PhylomeDB" id="Q58854"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01295">
        <property type="entry name" value="AdeC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01518">
        <property type="entry name" value="Adenine_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006679">
        <property type="entry name" value="Adenine_deam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026912">
        <property type="entry name" value="Adenine_deam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF2">
        <property type="entry name" value="PTHR11113:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13382">
        <property type="entry name" value="Adenine_deam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01178">
        <property type="entry name" value="ade"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142440" description="Adenine deaminase">
        <location>
            <begin position="1"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01518"/>
        </source>
    </evidence>
    <sequence length="556" mass="62623" checksum="06BD5E31E7EF5EFD" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58874</accession>
    <name>Y1479_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized aminotransferase MJ1479</fullName>
            <ecNumber>2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1479</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64484">
        <property type="entry name" value="F64484"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871000.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58874"/>
    <dbReference type="SMR" id="Q58874"/>
    <dbReference type="STRING" id="243232.MJ_1479"/>
    <dbReference type="EnsemblBacteria" id="AAB99491">
        <property type="protein sequence ID" value="AAB99491"/>
        <property type="gene ID" value="MJ_1479"/>
    </dbReference>
    <dbReference type="GeneID" id="1452384"/>
    <dbReference type="KEGG" id="mja:MJ_1479"/>
    <dbReference type="eggNOG" id="arCOG01135">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_2_2"/>
    <dbReference type="InParanoid" id="Q58874"/>
    <dbReference type="OMA" id="FGFECPP"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="PhylomeDB" id="Q58874"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000163832" description="Uncharacterized aminotransferase MJ1479">
        <location>
            <begin position="1"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="432" mass="49430" checksum="14D1602E32DB31FE" modified="1996-11-01" version="1">MRNPIIDVGAKELSYEIREIVDVAKKIEEFGINITWENIGDPVAKGEKIPDWIKDIIAEIVKNDCSYAYCPTKGLLETREFLAEQVNKRGGVQITAEDIIFFNGLGDAIAKIYGLLKRQVRVINPSPSYSTHSSAEASHAGSPPVTYFLDPYNYWYPDIDDLEKRIKYNPAVSGILVINPDNPTGAVYPKKILNEIVDLANEYDLFIICDEIYCNLVYNGKKQHLLCEVIDDVCGLSLKGISKELPWPGARCGWIEIYNADKDEEFKKYVESIYKAKLIEVCSTTLPQMAIPRIMGHRNYKKYLEERNRFFEKRSNTAYKKLKDLDGVIANKANGAFYMSVVFEDNYLNGNNSIKIENEKLKEFIEHQIKDASIDKKFVYYLLASTGICVVPLTSFCSQLNGFRVTLLERDDEKFEWIFDTLAEKIDEFLKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58893</accession>
    <name>MTM5_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II methyltransferase M.MjaV</fullName>
            <shortName evidence="1">M.MjaV</shortName>
            <ecNumber>2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N-4 cytosine-specific methyltransferase MjaV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaVM</name>
        <name type="ordered locus">MJ1498</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="patent" date="1999-03-11" number="WO9911821">
            <title>Method for screening restriction endonucleases.</title>
            <authorList>
                <person name="Noren C.J."/>
                <person name="Roberts R.J."/>
                <person name="Patti J."/>
                <person name="Byrd D.R."/>
                <person name="Morgan R.D."/>
            </authorList>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A beta subtype methylase that recognizes the double-stranded sequence 5'-GTAC-3', methylates C-4 on both strands, and protects the DNA from cleavage by the MjaV endonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64487">
        <property type="entry name" value="A64487"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871021.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58893"/>
    <dbReference type="SMR" id="Q58893"/>
    <dbReference type="STRING" id="243232.MJ_1498"/>
    <dbReference type="REBASE" id="3897">
        <property type="entry name" value="M.MjaV"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q58893"/>
    <dbReference type="EnsemblBacteria" id="AAB99509">
        <property type="protein sequence ID" value="AAB99509"/>
        <property type="gene ID" value="MJ_1498"/>
    </dbReference>
    <dbReference type="GeneID" id="1452405"/>
    <dbReference type="KEGG" id="mja:MJ_1498"/>
    <dbReference type="eggNOG" id="arCOG00115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024927_2_3_2"/>
    <dbReference type="InParanoid" id="Q58893"/>
    <dbReference type="OMA" id="YFRDARK"/>
    <dbReference type="OrthoDB" id="63009at2157"/>
    <dbReference type="PhylomeDB" id="Q58893"/>
    <dbReference type="PRO" id="PR:Q58893"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000087938" description="Type II methyltransferase M.MjaV">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="292" mass="33877" checksum="A5A46D7C8FBD3FE3" modified="1996-11-01" version="1">MEINKIYCMDCLEGMKQLKDKTVDVVVTSPPYNIGIKYNKYSDNLSREDYLNWIEEVVKEIKRVLKDDGSFFINVGYTAKDPWIAFDVANVIRKHFKLQNTIHWVKSIAIQKEDVGNYPNIIGDIAVGHYKPINSDRFLSIMHEYIFHFTKNGNVKLDKLAIGVPYQDKSNIKRFNRKGDLRDRGNTWFIPYETIQSKEKERPHPATFPPKLPEMCIKLHGVKKTNLVLDPFMGIGSTAIACIRLGIDYIGFEIDEYYCRVAEERIKKELLKTDGKFDNVKNKNIITLDAFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58902</accession>
    <name>Y1507_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter permease MJ1507</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1507</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC-4 integral membrane protein family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64488">
        <property type="entry name" value="B64488"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58902"/>
    <dbReference type="SMR" id="Q58902"/>
    <dbReference type="STRING" id="243232.MJ_1507"/>
    <dbReference type="PRIDE" id="Q58902"/>
    <dbReference type="EnsemblBacteria" id="AAB99519">
        <property type="protein sequence ID" value="AAB99519"/>
        <property type="gene ID" value="MJ_1507"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1507"/>
    <dbReference type="eggNOG" id="arCOG02312">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_8_0_2"/>
    <dbReference type="InParanoid" id="Q58902"/>
    <dbReference type="OMA" id="GIMNITF"/>
    <dbReference type="PhylomeDB" id="Q58902"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003838">
        <property type="entry name" value="ABC3_permease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025857">
        <property type="entry name" value="MacB_PCD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02687">
        <property type="entry name" value="FtsX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12704">
        <property type="entry name" value="MacB_PCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000107382" description="Uncharacterized ABC transporter permease MJ1507">
        <location>
            <begin position="1"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="324"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="399" mass="44008" checksum="18A17826BBB25297" modified="1997-11-01" version="1">MGKSMKVDDIITFAFKNIKQKRTQSLLTIIGIVIGVLAMVSLISLGYGVQNYIHEEMMKMGSNKITILPMKQFGVPPSHLFTKKEIKAIKNVKGVDTVMYGWYGGCEIEYNGEKKFVSYYYAIPSKLREVYKDSGYDIEEGRWLEDNDKYACVIGYGTAHNLFDREIKVGDVIKIKDKKFRVVGILKQIGNQQDDNSIILNIDVGEKLFGNEGKYNFISVTVKEGEDIEKVSEEIKKALKKSFGDEDFSVLTAEQLAKTVSSVLGVITIFVVGVAAISLLVGAVGISNTMHMSILERRKDIGILKALGAETTDILAIFVVESGFLGLFGGIVGLVLGILLAEVIEALAHKMGYLMVNAWISWELIVGVLIFSFLVGVISGYFPARSGAKLNPIETLRGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58906</accession>
    <name>Y1511_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1511</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1511</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64488">
        <property type="entry name" value="F64488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871034.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58906"/>
    <dbReference type="SMR" id="Q58906"/>
    <dbReference type="STRING" id="243232.MJ_1511"/>
    <dbReference type="EnsemblBacteria" id="AAB99530">
        <property type="protein sequence ID" value="AAB99530"/>
        <property type="gene ID" value="MJ_1511"/>
    </dbReference>
    <dbReference type="GeneID" id="1452418"/>
    <dbReference type="KEGG" id="mja:MJ_1511"/>
    <dbReference type="eggNOG" id="arCOG02148">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_137228_5_0_2"/>
    <dbReference type="OMA" id="FYEAVAN"/>
    <dbReference type="OrthoDB" id="92755at2157"/>
    <dbReference type="PhylomeDB" id="Q58906"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="SSF69118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107384" description="Uncharacterized protein MJ1511">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <sequence length="107" mass="11891" checksum="E93B27785FFF7C82" modified="1996-11-01" version="1">MAEFVPAETIEVVKKNCPEFYEAVANLQKEVFSGKKLDEKMQRLVLLAVVATLGDEKAVKRQTKKLMEMGATIEEIQDVMKVVYIGAGMPRFIKAVEAILEVAGDQC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-29" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q58928</accession>
    <name>Y1533_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized KH and PIN-domain containing protein MJ1533</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1533</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2009-07" db="PDB data bank">
            <title>A domain of a functionally unknown protein from Methanocaldococcus jannaschii DSM 2661.</title>
            <authorList>
                <consortium name="Midwest center for structural genomics (MCSG)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.64 ANGSTROMS) OF 27-165</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the PINc/VapC protein family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64491">
        <property type="entry name" value="D64491"/>
    </dbReference>
    <dbReference type="PDB" id="3I8O">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.64 A"/>
        <property type="chains" value="A=27-165"/>
    </dbReference>
    <dbReference type="PDBsum" id="3I8O"/>
    <dbReference type="AlphaFoldDB" id="Q58928"/>
    <dbReference type="SMR" id="Q58928"/>
    <dbReference type="STRING" id="243232.MJ_1533"/>
    <dbReference type="PRIDE" id="Q58928"/>
    <dbReference type="DNASU" id="1452441"/>
    <dbReference type="EnsemblBacteria" id="AAB99554">
        <property type="protein sequence ID" value="AAB99554"/>
        <property type="gene ID" value="MJ_1533"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1533"/>
    <dbReference type="eggNOG" id="arCOG04116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023387_0_0_2"/>
    <dbReference type="InParanoid" id="Q58928"/>
    <dbReference type="OMA" id="FADMRMA"/>
    <dbReference type="PhylomeDB" id="Q58928"/>
    <dbReference type="EvolutionaryTrace" id="Q58928"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000050166" description="Uncharacterized KH and PIN-domain containing protein MJ1533">
        <location>
            <begin position="1"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="PINc">
        <location>
            <begin position="29"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="2">
        <location>
            <begin position="510"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="28"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="33"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="40"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="47"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="97"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="106"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="114"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="131"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="137"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="150"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3I8O"/>
        </source>
    </evidence>
    <sequence length="642" mass="72907" checksum="9CA730BFA10ABE69" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58963</accession>
    <name>THPR_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="1">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="1">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1568</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64495">
        <property type="entry name" value="G64495"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871092.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58963"/>
    <dbReference type="SMR" id="Q58963"/>
    <dbReference type="STRING" id="243232.MJ_1568"/>
    <dbReference type="EnsemblBacteria" id="AAB99586">
        <property type="protein sequence ID" value="AAB99586"/>
        <property type="gene ID" value="MJ_1568"/>
    </dbReference>
    <dbReference type="GeneID" id="1452476"/>
    <dbReference type="KEGG" id="mja:MJ_1568"/>
    <dbReference type="eggNOG" id="arCOG01736">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081251_3_4_2"/>
    <dbReference type="InParanoid" id="Q58963"/>
    <dbReference type="OMA" id="HITLRFL"/>
    <dbReference type="OrthoDB" id="102399at2157"/>
    <dbReference type="PhylomeDB" id="Q58963"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:2'-5'-RNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010738">
        <property type="term" value="P:regulation of protein kinase A signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014051">
        <property type="entry name" value="Phosphoesterase_HXTX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="PTHR35561"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02834">
        <property type="entry name" value="LigT_PEase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="SSF55144"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138963" description="RNA 2',3'-cyclic phosphodiesterase">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="1">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="1">
        <location>
            <begin position="118"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <sequence length="173" mass="20545" checksum="34F03BCE0159E5B1" modified="1996-11-01" version="1">MRLFLAIDIPENIKEEIAKFQEQFKMKGIKLVEKENLHITVKFLGEVDEEKLKEILNLDLSIQPIKIKLKYIGTFPNSNYIRVIWIGAYNNNLVEIFKEIDEKLSNLGFKKEREYVPHLTIGRVKFIDNKKKLKDRIEKYKDVDFGEFEAKHIKLYKSTLTPNGPIYEVIKEW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58969</accession>
    <name>Y1574_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-dependent helicase MJ1574</fullName>
            <ecNumber>3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1574</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64496">
        <property type="entry name" value="E64496"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871099.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58969"/>
    <dbReference type="SMR" id="Q58969"/>
    <dbReference type="STRING" id="243232.MJ_1574"/>
    <dbReference type="EnsemblBacteria" id="AAB99590">
        <property type="protein sequence ID" value="AAB99590"/>
        <property type="gene ID" value="MJ_1574"/>
    </dbReference>
    <dbReference type="GeneID" id="1452483"/>
    <dbReference type="KEGG" id="mja:MJ_1574"/>
    <dbReference type="eggNOG" id="arCOG00555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000809_3_2_2"/>
    <dbReference type="InParanoid" id="Q58969"/>
    <dbReference type="PhylomeDB" id="Q58969"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102200" description="Uncharacterized ATP-dependent helicase MJ1574">
        <location>
            <begin position="1"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="54"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="264"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEVH box">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="67"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="684" mass="81280" checksum="B60E8AFC0C6A49AE" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58988</accession>
    <name>PURS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="2">FGAM synthase</shortName>
            <ecNumber evidence="2">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="2">FGAR amidotransferase III</shortName>
            <shortName evidence="2">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purS</name>
        <name type="ordered locus">MJ1593</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2011-07" db="PDB data bank">
            <title>Crystal structure of Methanocaldococcus jannaschII purs, one of the subunits of formylglycinamide ribonucleotide amidotransferase in the purine biosynthetic pathway.</title>
            <authorList>
                <consortium name="RIKEN structural genomics initiative (RSGI)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PurS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64498">
        <property type="entry name" value="H64498"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871117.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2YX5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-83"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="Q58988"/>
    <dbReference type="SMR" id="Q58988"/>
    <dbReference type="STRING" id="243232.MJ_1593"/>
    <dbReference type="PRIDE" id="Q58988"/>
    <dbReference type="EnsemblBacteria" id="AAB99611">
        <property type="protein sequence ID" value="AAB99611"/>
        <property type="gene ID" value="MJ_1593"/>
    </dbReference>
    <dbReference type="GeneID" id="1452501"/>
    <dbReference type="KEGG" id="mja:MJ_1593"/>
    <dbReference type="eggNOG" id="arCOG04462">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_164833_3_0_2"/>
    <dbReference type="InParanoid" id="Q58988"/>
    <dbReference type="OMA" id="PVIHDYE"/>
    <dbReference type="OrthoDB" id="117509at2157"/>
    <dbReference type="PhylomeDB" id="Q58988"/>
    <dbReference type="BRENDA" id="6.3.5.3">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58988"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PTHR34696"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="SSF82697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00302">
        <property type="entry name" value="TIGR00302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000100399" description="Phosphoribosylformylglycinamidine synthase subunit PurS">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="41"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="73"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2YX5"/>
        </source>
    </evidence>
    <sequence length="83" mass="9696" checksum="461F8DDB0DF0B808" modified="1996-11-01" version="1">MYKATVIIKLKKGVLNPEGRTIQRALNFLGFNNVKEVQTYKMIDIIMEGENEEKVKEEVEEMCKKLLANPVIHDYEIKVEKIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>Q58989</accession>
    <name>SERB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoserine phosphatase</fullName>
            <shortName>PSP</shortName>
            <shortName>PSPase</shortName>
            <ecNumber evidence="2 3">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1594</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Proc. Natl. Acad. Sci. U.S.A." volume="98" first="8525" last="8530">
            <title>BeF(3)(-) acts as a phosphate analog in proteins phosphorylated on aspartate: structure of a BeF(3)(-) complex with phosphoserine phosphatase.</title>
            <authorList>
                <person name="Cho H."/>
                <person name="Wang W."/>
                <person name="Kim R."/>
                <person name="Yokota H."/>
                <person name="Damo S."/>
                <person name="Kim S.H."/>
                <person name="Wemmer D."/>
                <person name="Kustu S."/>
                <person name="Yan D."/>
            </authorList>
            <dbReference type="PubMed" id="11438683"/>
            <dbReference type="DOI" id="10.1073/pnas.131213698"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH BEF3 AND MAGNESIUM ION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVE SITE</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2001" name="Structure" volume="9" first="65" last="71">
            <title>Crystal structure of phosphoserine phosphatase from Methanococcus jannaschii, a hyperthermophile, at 1.8 A resolution.</title>
            <authorList>
                <person name="Wang W."/>
                <person name="Kim R."/>
                <person name="Jancarik J."/>
                <person name="Yokota H."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="11342136"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(00)00558-X"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS AND PHOSPHATE</scope>
        <scope>ACTIVE SITE</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2002" name="J. Mol. Biol." volume="319" first="421" last="431">
            <title>Structural characterization of the reaction pathway in phosphoserine phosphatase: crystallographic 'snapshots' of intermediate states.</title>
            <authorList>
                <person name="Wang W."/>
                <person name="Cho H.S."/>
                <person name="Kim R."/>
                <person name="Jancarik J."/>
                <person name="Yokota H."/>
                <person name="Nguyen H.H."/>
                <person name="Grigoriev I.V."/>
                <person name="Wemmer D.E."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="12051918"/>
            <dbReference type="DOI" id="10.1016/S0022-2836(02)00324-8"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.48 ANGSTROMS) IN COMPLEXES WITH MAGNESIUM; SUBSTRATE; PHOSPHATE AND TRANSITION STATE ANALOG</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ENZYME MECHANISM</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVE SITE</scope>
        <scope>MUTAGENESIS OF ASP-11</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H2O + O-phospho-L-serine = L-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H2O + O-phospho-D-serine = D-serine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24873"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35247"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58680"/>
            <dbReference type="EC" id="3.1.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">620 uM for O-phospho-L-serine (at 70 degrees Celsius and at pH 7.5)</KM>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 7.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 70 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="2 3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64499">
        <property type="entry name" value="A64499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871118.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1F5S">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-211"/>
    </dbReference>
    <dbReference type="PDB" id="1J97">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="A/B=1-211"/>
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</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q59054</accession>
    <name>SEPS_METJA</name>
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        <recommendedName>
            <fullName>O-phosphoserine--tRNA(Cys) ligase</fullName>
            <shortName>O-phosphoserine--tRNA ligase</shortName>
            <ecNumber>6.1.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Non-canonical O-phosphoseryl-tRNA(Cys) synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>O-phosphoseryl-tRNA(Cys) synthetase</fullName>
            <shortName>SepRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">sepS</name>
        <name type="ordered locus">MJ1660</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Science" volume="307" first="1969" last="1972">
            <title>RNA-dependent cysteine biosynthesis in archaea.</title>
            <authorList>
                <person name="Sauerwald A."/>
                <person name="Zhu W."/>
                <person name="Major T.A."/>
                <person name="Roy H."/>
                <person name="Palioura S."/>
                <person name="Jahn D."/>
                <person name="Whitman W.B."/>
                <person name="Yates J.R. III"/>
                <person name="Ibba M."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="15790858"/>
            <dbReference type="DOI" id="10.1126/science.1108329"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="Nat. Struct. Mol. Biol." volume="15" first="507" last="514">
            <title>Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys).</title>
            <authorList>
                <person name="Zhang C.-M."/>
                <person name="Liu C."/>
                <person name="Slater S."/>
                <person name="Hou Y.-M."/>
            </authorList>
            <dbReference type="PubMed" id="18425141"/>
            <dbReference type="DOI" id="10.1038/nsmb.1423"/>
        </citation>
        <scope>INTERACTION WITH SEPCYSS</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2007" name="Nat. Struct. Mol. Biol." volume="14" first="272" last="279">
            <title>Structural insights into the first step of RNA-dependent cysteine biosynthesis in archaea.</title>
            <authorList>
                <person name="Fukunaga R."/>
                <person name="Yokoyama S."/>
            </authorList>
            <dbReference type="PubMed" id="17351629"/>
            <dbReference type="DOI" id="10.1038/nsmb1219"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + O-phospho-L-serine + tRNA(Cys) = AMP + diphosphate + O-phospho-L-seryl-tRNA(Cys)</text>
            <dbReference type="Rhea" id="RHEA:25678"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
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        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="4">0.7 uM for tRNA(Cys) (at 60 degrees Celsius and pH 6.0)</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homotetramer. Interacts with SepCysS.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15698391">
            <id>Q59054</id>
        </interactant>
        <interactant intactId="EBI-15698426">
            <id>Q59072</id>
            <label>pscS</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>4</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="5">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99678" version="1"/>
        </conflict>
    </comment>
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</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
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            <fullName>Uncharacterized protein MJ1673</fullName>
        </recommendedName>
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
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            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
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            <taxon>Methanocaldococcus</taxon>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
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                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
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                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
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                <person name="Cotton M.D."/>
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                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
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                <person name="Fraser C.M."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2 5-aminolevulinate = H(+) + 2 H2O + porphobilinogen</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
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        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per monomer.</text>
    </comment>
    <comment type="pathway">
        <text>Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ALAD family.</text>
    </comment>
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    <dbReference type="InParanoid" id="Q60178"/>
    <dbReference type="OMA" id="YQMDYAN"/>
    <dbReference type="PhylomeDB" id="Q60178"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="PTHR11458"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0350">Heme biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000140523" description="Delta-aminolevulinic acid dehydratase">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="335" mass="37396" checksum="4D097DAB45F3705D" modified="1997-11-01" version="1">MFKINLGDFMLIRPRRLRKNQKIRDLVRETILTKNDLIMPIFVDENLKGNEKKEISSMPNQYRFSVEGAIEEAKEIADLGIPAVILFGIPKHKDEIASSAYDKNGVVQRTIRGIKEELGDELLVIADCCLCEYTSHGHCGIVKDGKILNDATLPILAKIALSYADAGVDIVAPSDMMDGRVRAIREILEENGYDDVAIMSYSAKYASSFYGPFREAAESAPKFGDRKSYQMDIGNAREALKEIALDIEEGADLILVKPALPYLDIIRMAKDRFDVPIGGYCVSGEYAMVEAAARNGWLDREKVIYEILLSIKRAGADFIITYWAKEVAEIGLSQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q60308</accession>
    <name>Y3409_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS09</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS09</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64517">
        <property type="entry name" value="A64517"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890102.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60308"/>
    <dbReference type="STRING" id="243232.MJ_ECS09"/>
    <dbReference type="EnsemblBacteria" id="AAC37067">
        <property type="protein sequence ID" value="AAC37067"/>
        <property type="gene ID" value="MJ_ECS09"/>
    </dbReference>
    <dbReference type="GeneID" id="1450836"/>
    <dbReference type="KEGG" id="mja:MJ_ECS09"/>
    <dbReference type="HOGENOM" id="CLU_1168579_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107490" description="Uncharacterized protein MJECS09">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <sequence length="237" mass="28011" checksum="06A87D28E3683F85" modified="1997-11-01" version="1">MPLPEYGTDNSDNFVRFIYFEIKKNSVKPPSFPKLGKYLENHCKGGDYMCVINNYIYFLIIIDLEYLVKWIKCYSDNVLKGTIKLLTNYYSKLYYIKLSLSHWLFSLCNLPLEDEMLFLSCGSLVKYQDASTHKLYKLEYLTKLILGYNRINVDNCICSIKVFAISTFERFLYHEFENNAFKLIMLHKRTSFNVYKVKTSKIALLNILEKIGRTYKRYLHTLKTPKNLTTTEVVRHG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q60313</accession>
    <name>Y003_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0003</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0003</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64300">
        <property type="entry name" value="C64300"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869496.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60313"/>
    <dbReference type="STRING" id="243232.MJ_0003"/>
    <dbReference type="EnsemblBacteria" id="AAB97990">
        <property type="protein sequence ID" value="AAB97990"/>
        <property type="gene ID" value="MJ_0003"/>
    </dbReference>
    <dbReference type="GeneID" id="1450842"/>
    <dbReference type="KEGG" id="mja:MJ_0003"/>
    <dbReference type="eggNOG" id="arCOG02433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120866_1_0_2"/>
    <dbReference type="InParanoid" id="Q60313"/>
    <dbReference type="OMA" id="PCPELIY"/>
    <dbReference type="OrthoDB" id="88086at2157"/>
    <dbReference type="PhylomeDB" id="Q60313"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106646" description="Uncharacterized protein MJ0003">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <sequence length="156" mass="18429" checksum="364797DB0EE38AB9" modified="1997-11-01" version="1">MKGKRIAIVSHRILNQNSVVNGLERAEGAFNEVVEILLKNNYGIIQLPCPELIYLGIDREGKTKEEYDTKEYRELCKKLLEPIIKYLQEYKKDNYKFILIGIENSTTCDIFKNRGILMEEFFKEVEKLNIIIKAIEYPKNEKDYNKFVKTLEKMIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q60316</accession>
    <name>FDHB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Formate dehydrogenase subunit beta</fullName>
            <ecNumber>1.17.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fdhB</name>
        <name type="ordered locus">MJ0005</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of formate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>formate + NAD(+) = CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:15985"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Dimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FrhB family.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.9"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64300">
        <property type="entry name" value="E64300"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60316"/>
    <dbReference type="SMR" id="Q60316"/>
    <dbReference type="STRING" id="243232.MJ_0005"/>
    <dbReference type="EnsemblBacteria" id="AAB97986">
        <property type="protein sequence ID" value="AAB97986"/>
        <property type="gene ID" value="MJ_0005"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0005"/>
    <dbReference type="eggNOG" id="arCOG02653">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063409_0_0_2"/>
    <dbReference type="InParanoid" id="Q60316"/>
    <dbReference type="OMA" id="CAPTMFG"/>
    <dbReference type="PhylomeDB" id="Q60316"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009326">
        <property type="term" value="C:formate dehydrogenase complex"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159224" description="Formate dehydrogenase subunit beta">
        <location>
            <begin position="1"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="271"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="321"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="379" mass="43014" checksum="9C257CCAD5547F5A" modified="1997-11-01" version="1">MKYVLIQATDNGILRRAECGGAVTALFKYLLDKKLVDGVLALKRGEDVYDGIPTFITNSNELVETAGSLHCAPTNFGKLIAKYLADKKIAVPAKPCDAMAIRELAKLNQINLDNVYMIGLNCGGTISPITAMKMIELFYEVNPLDVVKEEIDKGKFIIELKNGEHKAVKIEELEEKGFGRRKNCQRCEIMIPRMADLACGNWGAEKGWTFVEICSERGRKLVEDAEKDGYIKIKQPSEKAIQVREKIESIMIKLAKKFQKKHLEEEYPSLEKWKKYWNRCIKCYGCRDNCPLCFCVECSLEKDYIEEKGKIPPNPLIFQGIRLSHISQSCINCGQCEDACPMDIPLAYIFHRMQLKIRDTLGYIPGVDNSLPPLFNIER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q60324</accession>
    <name>Y018_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0018</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0018</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64302">
        <property type="entry name" value="B64302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869510.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60324"/>
    <dbReference type="EnsemblBacteria" id="AAB98002">
        <property type="protein sequence ID" value="AAB98002"/>
        <property type="gene ID" value="MJ_0018"/>
    </dbReference>
    <dbReference type="GeneID" id="1450856"/>
    <dbReference type="KEGG" id="mja:MJ_0018"/>
    <dbReference type="eggNOG" id="arCOG09657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_519373_0_0_2"/>
    <dbReference type="OrthoDB" id="143803at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106658" description="Uncharacterized protein MJ0018">
        <location>
            <begin position="1"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="524" mass="56565" checksum="0F878499773FF87C" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q60341</accession>
    <name>FRHB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Coenzyme F420 hydrogenase subunit beta</fullName>
            <ecNumber>1.12.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>8-hydroxy-5-deazaflavin-reducing hydrogenase subunit beta</fullName>
            <shortName>FRH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">frhB</name>
        <name type="ordered locus">MJ0032</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>H(+) + H2 + oxidized coenzyme F420-(gamma-L-Glu)(n) = reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:23760"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.12.98.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>iron-sulfur cluster</name>
            <dbReference type="ChEBI" id="CHEBI:30408"/>
        </cofactor>
        <text evidence="1">There are 12-13 Fe atoms per alpha/beta/gamma unit of the FRH.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterocomplex of the form (alpha(1)beta(1)gamma(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FrhB family.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64303">
        <property type="entry name" value="H64303"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60341"/>
    <dbReference type="SMR" id="Q60341"/>
    <dbReference type="STRING" id="243232.MJ_0032"/>
    <dbReference type="EnsemblBacteria" id="AAB98011">
        <property type="protein sequence ID" value="AAB98011"/>
        <property type="gene ID" value="MJ_0032"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0032"/>
    <dbReference type="eggNOG" id="arCOG02651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="InParanoid" id="Q60341"/>
    <dbReference type="OMA" id="REYGCEK"/>
    <dbReference type="PhylomeDB" id="Q60341"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017679">
        <property type="entry name" value="FrhB_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03289">
        <property type="entry name" value="frhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159226" description="Coenzyme F420 hydrogenase subunit beta">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="287" mass="32054" checksum="7CE7D4E6E970940E" modified="1997-11-01" version="1">MRWNRMNPFGSYKKVVSARSTLKEVLKKAQDGGIVSTAFIYGLENNLLDGVIVADNAGEFKAVPKVATTPEEVLEAAGTKYTVCPNISVLKSAVREYGCEKIGVVGTPCQVRAVRKLMKYPVGFRHVPDKIALIIGIFCMENFPYYGLKLIVEEHCGVKMEDVVKMDIGKGKFWVYTRWGETKAIKLKETHPYEQIACHVCTDYTAELADISTGSVGSPDGWSTVFIRTAKGEEIFNKMVEDGYLEVKPIEEVKPGLGLVEKLALQKKEKNMKEIEHRKELGLPIPY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q60356</accession>
    <name>Y033_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized FAD-dependent oxidoreductase MJ0033</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0033</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64304">
        <property type="entry name" value="A64304"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869524.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60356"/>
    <dbReference type="SMR" id="Q60356"/>
    <dbReference type="STRING" id="243232.MJ_0033"/>
    <dbReference type="PRIDE" id="Q60356"/>
    <dbReference type="EnsemblBacteria" id="AAB98014">
        <property type="protein sequence ID" value="AAB98014"/>
        <property type="gene ID" value="MJ_0033"/>
    </dbReference>
    <dbReference type="GeneID" id="1450871"/>
    <dbReference type="KEGG" id="mja:MJ_0033"/>
    <dbReference type="eggNOG" id="arCOG00571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_8_1_2"/>
    <dbReference type="InParanoid" id="Q60356"/>
    <dbReference type="OMA" id="PTAHHFM"/>
    <dbReference type="OrthoDB" id="8547at2157"/>
    <dbReference type="PhylomeDB" id="Q60356"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="PTHR11632"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="SSF46977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="SSF56425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158670" description="Uncharacterized FAD-dependent oxidoreductase MJ0033">
        <location>
            <begin position="1"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="6"/>
            <end position="20"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="539" mass="60398" checksum="89FF7AE3D6268795" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q60359</accession>
    <name>Y052_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0052</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0052</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64306">
        <property type="entry name" value="D64306"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869544.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60359"/>
    <dbReference type="SMR" id="Q60359"/>
    <dbReference type="STRING" id="243232.MJ_0052"/>
    <dbReference type="DNASU" id="1450891"/>
    <dbReference type="EnsemblBacteria" id="AAB98033">
        <property type="protein sequence ID" value="AAB98033"/>
        <property type="gene ID" value="MJ_0052"/>
    </dbReference>
    <dbReference type="GeneID" id="1450891"/>
    <dbReference type="KEGG" id="mja:MJ_0052"/>
    <dbReference type="eggNOG" id="arCOG02020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1237913_0_0_2"/>
    <dbReference type="InParanoid" id="Q60359"/>
    <dbReference type="OMA" id="FEPVNIK"/>
    <dbReference type="OrthoDB" id="64335at2157"/>
    <dbReference type="PhylomeDB" id="Q60359"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030818">
        <property type="entry name" value="Arch_SelU_Nterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="SSF52821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04568">
        <property type="entry name" value="arch_SelU_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106670" description="Uncharacterized protein MJ0052">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="104"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00173"/>
        </source>
    </evidence>
    <sequence length="222" mass="25151" checksum="61991060852008A6" modified="1997-11-01" version="1">MDEGILARLITFTEDVVLCIVLNDGRKMITNGKKILAGKIEGELASFILSASKEFLEDKKVGVKKFKDYDIYFERIDINKFLKSIGGEFVKNTITVSELLELIKKEDVIIVDTRSPREFKEETLPGAINIPLFLDDEHALIGKTYKQESREKAIEIATDIVEKSLKRILNEAKKLDRDKLIVVFCARGGMRSQTMALILQLLGFKVKRLIGGFKAFKHAVDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q60364</accession>
    <name>RIBB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName>DHBP synthase</shortName>
            <shortName evidence="5">DHBPS</shortName>
            <ecNumber evidence="1">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ribB</name>
        <name type="ordered locus">MJ0055</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Biol. Chem." volume="277" first="41410" last="41416">
            <title>Biosynthesis of riboflavin in archaea studies on the mechanism of 3,4-dihydroxy-2-butanone-4-phosphate synthase of Methanococcus jannaschii.</title>
            <authorList>
                <person name="Fischer M."/>
                <person name="Romisch W."/>
                <person name="Schiffmann S."/>
                <person name="Kelly M."/>
                <person name="Oschkinat H."/>
                <person name="Steinbacher S."/>
                <person name="Huber R."/>
                <person name="Eisenreich W."/>
                <person name="Richter G."/>
                <person name="Bacher A."/>
            </authorList>
            <dbReference type="PubMed" id="12200440"/>
            <dbReference type="DOI" id="10.1074/jbc.M206863200"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-15</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>COFACTOR</scope>
        <scope>PATHWAY</scope>
        <scope>MUTAGENESIS OF ASP-21; ARG-25; GLU-26; GLU-28; ASP-30; CYS-55; THR-112; ARG-119; ARG-161; HIS-164; THR-165; GLU-166 AND GLU-185</scope>
    </reference>
    <reference evidence="10 11" key="3">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="42256" last="42265">
            <title>Structure of 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with divalent metal ions and the substrate ribulose 5-phosphate: implications for the catalytic mechanism.</title>
            <authorList>
                <person name="Steinbacher S."/>
                <person name="Schiffmann S."/>
                <person name="Richter G."/>
                <person name="Huber R."/>
                <person name="Bacher A."/>
                <person name="Fischer M."/>
            </authorList>
            <dbReference type="PubMed" id="12904291"/>
            <dbReference type="DOI" id="10.1074/jbc.M307301200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF WILD-TYPE AND MUTANT SER-147 IN COMPLEXES WITH ZINC; CALCIUM AND D-RIBULOSE 5-PHOSPHATE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="journal article" date="2004" name="Acta Crystallogr. D" volume="60" first="1338" last="1340">
            <title>Metal sites in 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with the substrate ribulose 5-phosphate.</title>
            <authorList>
                <person name="Steinbacher S."/>
                <person name="Schiffmann S."/>
                <person name="Bacher A."/>
                <person name="Fischer M."/>
            </authorList>
            <dbReference type="PubMed" id="15213409"/>
            <dbReference type="DOI" id="10.1107/S0907444904009862"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) OF MUTANT SER-147 IN COMPLEXES WITH ZINC; CALCIUM AND D-RIBULOSE 5-PHOSPHATE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:18458"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2 3 7 8 9">Requires divalent metal ions, preferentially magnesium, for activity (Probable). Binds 2 divalent metal cations per subunit (PubMed:12904291, PubMed:15213409). Zinc and calcium, which are present in the crystals do not support the enzymatic reaction.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">140 uM for ribulose-5-phosphate</KM>
            <Vmax evidence="1">148.0 nmol/min/mg enzyme</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3">Homodimer.</text>
    </comment>
    <comment type="mass spectrometry" mass="25799.0" method="Electrospray" evidence="1"/>
    <comment type="similarity">
        <text evidence="6">Belongs to the DHBP synthase family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB98035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="G64306"/>
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    <dbReference type="RefSeq" id="WP_010869547.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.45 A"/>
        <property type="chains" value="A/B/C=1-227"/>
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    <dbReference type="PDB" id="1PVY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
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        <property type="resolution" value="1.55 A"/>
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        <property type="protein sequence ID" value="AAB98035"/>
        <property type="gene ID" value="MJ_0055"/>
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    <dbReference type="GeneID" id="1450894"/>
    <dbReference type="KEGG" id="mja:MJ_0055"/>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_020273_3_2_2"/>
    <dbReference type="InParanoid" id="Q60364"/>
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        <property type="component" value="Chromosome"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="chain" id="PRO_0000151825" description="3,4-dihydroxy-2-butanone 4-phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2 11 12">
        <location>
            <begin position="25"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11 12">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11 12">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 11 12">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 11 12">
        <location>
            <begin position="161"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11 12">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity">
        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="site" description="Essential for catalytic activity">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="1">
        <original>D</original>
        <variation>E</variation>
        <variation>N</variation>
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 70-fold." evidence="1">
        <original>D</original>
        <variation>S</variation>
        <location>
            <position position="21"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 50-fold." evidence="1">
        <original>R</original>
        <variation>E</variation>
        <variation>K</variation>
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="1">
        <original>E</original>
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        <variation>Q</variation>
        <variation>S</variation>
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="1">
        <original>E</original>
        <variation>D</variation>
        <variation>S</variation>
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 23-fold." evidence="1">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="28"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="1">
        <original>D</original>
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        <variation>N</variation>
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 30-fold." evidence="1">
        <original>D</original>
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        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 70-fold. Increases Km for ribulose-5-phosphate 7-fold." evidence="1">
        <original>C</original>
        <variation>G</variation>
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 30-fold." evidence="1">
        <original>C</original>
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        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 50-fold. Increases Km for ribulose-5-phosphate 10-fold." evidence="1">
        <original>T</original>
        <variation>A</variation>
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 8-fold. Increases Km for ribulose-5-phosphate 8-fold." evidence="1">
        <original>R</original>
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        <location>
            <position position="119"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 10-fold.">
        <original>H</original>
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        <location>
            <position position="147"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity 10-fold. Increases Km for ribulose-5-phosphate 7-fold." evidence="1">
        <original>R</original>
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        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="1">
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        <variation>S</variation>
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity by 2-fold. Increases Km for ribulose-5-phosphate 8-fold." evidence="1">
        <original>T</original>
        <variation>A</variation>
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Reduces activity by 3-fold." evidence="1">
        <original>T</original>
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        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Increases Km for ribulose-5-phosphate 8-fold." evidence="1">
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        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="1">
        <original>E</original>
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        <variation>Q</variation>
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        <location>
            <position position="185"/>
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    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="3"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="17"/>
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    <feature type="turn" evidence="13">
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            <begin position="35"/>
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            <begin position="40"/>
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            <begin position="51"/>
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            <end position="66"/>
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    <feature type="helix" evidence="13">
        <location>
            <begin position="71"/>
            <end position="78"/>
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    <feature type="turn" evidence="13">
        <location>
            <begin position="79"/>
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    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="83"/>
            <end position="87"/>
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    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="96"/>
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    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="101"/>
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    <feature type="strand" evidence="13">
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            <begin position="138"/>
            <end position="141"/>
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            <begin position="142"/>
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            <begin position="157"/>
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    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="164"/>
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    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="179"/>
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    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="196"/>
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        </location>
    </feature>
    <feature type="strand" evidence="13">
        <location>
            <begin position="210"/>
            <end position="212"/>
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    </feature>
    <feature type="helix" evidence="13">
        <location>
            <begin position="213"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="12200440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12904291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="15213409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="12200440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="12904291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <evidence type="ECO:0000305" key="7">
        <source>
            <dbReference type="PubMed" id="12200440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="12904291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="9">
        <source>
            <dbReference type="PubMed" id="15213409"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="10">
        <source>
            <dbReference type="PDB" id="1PVW"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="11">
        <source>
            <dbReference type="PDB" id="1PVY"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="1SNN"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="13">
        <source>
            <dbReference type="PDB" id="1SNN"/>
        </source>
    </evidence>
    <sequence length="227" mass="25797" checksum="0718EE5B4247995D" modified="1997-11-01" version="1">MNNVEKAIEALKKGEIILVYDSDEREGETDMVVASQFITPEHIRIMRKDAGGLICTALHPDICNKLGIPFMVDILEFASQKFKVLRELYPNDIPYDEKSSFSITINHRKTFTGITDNDRAFTIKKLAELVKEGRFNDFGKEFRSPGHVTLLRAAEGLVKNRQGHTEMTVALAELANLVPITTICEMMGDDGNAMSKNETKRYAEKHNLIYLSGEEIINYYLDKYLKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q60368</accession>
    <name>FER1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0061</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0061</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64307">
        <property type="entry name" value="E64307"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869553.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60368"/>
    <dbReference type="SMR" id="Q60368"/>
    <dbReference type="STRING" id="243232.MJ_0061"/>
    <dbReference type="EnsemblBacteria" id="AAB98043">
        <property type="protein sequence ID" value="AAB98043"/>
        <property type="gene ID" value="MJ_0061"/>
    </dbReference>
    <dbReference type="GeneID" id="1450900"/>
    <dbReference type="KEGG" id="mja:MJ_0061"/>
    <dbReference type="eggNOG" id="arCOG00959">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476052_0_0_2"/>
    <dbReference type="InParanoid" id="Q60368"/>
    <dbReference type="OMA" id="CKNNVFA"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159129" description="Uncharacterized ferredoxin MJ0061">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="21"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="80" mass="9185" checksum="BE7C286649604918" modified="1997-11-01" version="1">MLSKILGIFKGKEKIEEKSNKIIEIDYNKCKNCLSCYRVCKNNVFAIKNNRVVVKNENNCTKCGECLKVCRYGAIILYDA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q60369</accession>
    <name>TSAC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative threonylcarbamoyl-AMP synthase</fullName>
            <shortName>TC-AMP synthase</shortName>
            <ecNumber>2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA threonylcarbamoyladenosine biosynthesis protein MJ0062</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0062</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64307">
        <property type="entry name" value="F64307"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869554.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60369"/>
    <dbReference type="SMR" id="Q60369"/>
    <dbReference type="STRING" id="243232.MJ_0062"/>
    <dbReference type="EnsemblBacteria" id="AAB98044">
        <property type="protein sequence ID" value="AAB98044"/>
        <property type="gene ID" value="MJ_0062"/>
    </dbReference>
    <dbReference type="GeneID" id="1450901"/>
    <dbReference type="KEGG" id="mja:MJ_0062"/>
    <dbReference type="eggNOG" id="arCOG01952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031397_3_2_2"/>
    <dbReference type="InParanoid" id="Q60369"/>
    <dbReference type="OMA" id="MLYPTDT"/>
    <dbReference type="OrthoDB" id="52427at2157"/>
    <dbReference type="PhylomeDB" id="Q60369"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="YrdC-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="SSF55821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00057">
        <property type="entry name" value="TIGR00057"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000202028" description="Putative threonylcarbamoyl-AMP synthase">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="YrdC-like" evidence="2">
        <location>
            <begin position="15"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="207" mass="23496" checksum="B7EF4298F2432774" modified="1997-11-01" version="1">MGLKNKIIKIYELNEEERKKVLEFLKKEILNGKIVICGTDTLYGISANALNEKAVRKVYNIKRREFNKPLSICVRDKNEIEKYAYVNDLAKKIIDKFLPGPLTIILKKKPGIPDIVAKDYIGIRIPDEPIIRELSIVPLTTTSANISGKESPTTVDEIDKEVLKKVDYVIDIGKCKYSKPSTIIKIEDDKIISIREGVIPIQKLARC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>P54024</accession>
    <name>RS9_METJA</name>
    <protein>
        <recommendedName>
            <fullName>30S ribosomal protein S9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rps9</name>
        <name type="ordered locus">MJ0195</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64324">
        <property type="entry name" value="D64324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869690.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54024"/>
    <dbReference type="SMR" id="P54024"/>
    <dbReference type="STRING" id="243232.MJ_0195"/>
    <dbReference type="EnsemblBacteria" id="AAB98175">
        <property type="protein sequence ID" value="AAB98175"/>
        <property type="gene ID" value="MJ_0195"/>
    </dbReference>
    <dbReference type="GeneID" id="8803917"/>
    <dbReference type="KEGG" id="mja:MJ_0195"/>
    <dbReference type="eggNOG" id="arCOG04243">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_2"/>
    <dbReference type="InParanoid" id="P54024"/>
    <dbReference type="OMA" id="FMGQAEA"/>
    <dbReference type="OrthoDB" id="115425at2157"/>
    <dbReference type="PhylomeDB" id="P54024"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_S9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_S9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_S9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_S9_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="PTHR21569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000111465" description="30S ribosomal protein S9">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="136" mass="15246" checksum="8AC2A9A7E67994C4" modified="1996-10-01" version="1">MGKIVITVGKRKRAIARAVAREGKGRIRINKIPIELIEPKYKRMKLMEPILLAGEEVISQMDIDVTVKGGGVMGQMDAARTAIGKAIVEFTGSKELRDKFLAYDRTLLVSDARRTEPHKPSRSTKGPRAKRQKSYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>P54050</accession>
    <name>RL1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">50S ribosomal protein L1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rpl1</name>
        <name type="ordered locus">MJ0510</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Eur. J. Biochem." volume="256" first="97" last="105">
            <title>Interaction of ribosomal L1 proteins from mesophilic and thermophilic Archaea and Bacteria with specific L1-binding sites on 23S rRNA and mRNA.</title>
            <authorList>
                <person name="Koehrer C."/>
                <person name="Mayer C."/>
                <person name="Neumair O."/>
                <person name="Groebner P."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="9746351"/>
            <dbReference type="DOI" id="10.1046/j.1432-1327.1998.2560097.x"/>
        </citation>
        <scope>BINDING TO ENDOGENOUS 23S RRNA</scope>
        <scope>BINDING TO METHANOCOCCUS VANNIELII L1 MRNA</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="Structure" volume="8" first="363" last="371">
            <title>Archaeal ribosomal protein L1: the structure provides new insights into RNA binding of the L1 protein family.</title>
            <authorList>
                <person name="Nevskaya N."/>
                <person name="Tischenko S."/>
                <person name="Fedorov R."/>
                <person name="Al-Karadaghi S."/>
                <person name="Liljas A."/>
                <person name="Kraft A."/>
                <person name="Piendl W."/>
                <person name="Garber M.B."/>
                <person name="Nikonov S."/>
            </authorList>
            <dbReference type="PubMed" id="10801481"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(00)00116-7"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2002" name="Acta Crystallogr. D" volume="58" first="1023" last="1029">
            <title>Structure of ribosomal protein L1 from Methanococcus thermolithotrophicus. Functionally important structural invariants on the L1 surface.</title>
            <authorList>
                <person name="Nevskaya N."/>
                <person name="Tishchenko S."/>
                <person name="Paveliev M."/>
                <person name="Smolinskaya Y."/>
                <person name="Fedorov R."/>
                <person name="Piendl W."/>
                <person name="Nakamura Y."/>
                <person name="Toyoda T."/>
                <person name="Garber M.B."/>
                <person name="Nikonov S."/>
            </authorList>
            <dbReference type="PubMed" id="12037305"/>
            <dbReference type="DOI" id="10.1107/S0907444902006157"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS)</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="478" last="485">
            <title>Ribosomal protein L1 recognizes the same specific structural motif in its target sites on the autoregulatory mRNA and 23S rRNA.</title>
            <authorList>
                <person name="Nevskaya N."/>
                <person name="Tishchenko S."/>
                <person name="Gabdoulkhakov A."/>
                <person name="Nikonova E."/>
                <person name="Nikonov O."/>
                <person name="Nikulin A."/>
                <person name="Platonova O."/>
                <person name="Garber M.B."/>
                <person name="Nikonov S."/>
                <person name="Piendl W."/>
            </authorList>
            <dbReference type="PubMed" id="15659579"/>
            <dbReference type="DOI" id="10.1093/nar/gki194"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) IN COMPLEX WITH AN ENDOGENOUS L1 MRNA FRAGMENT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in E site tRNA release (By similarity). Binds directly to 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98500" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98500.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64363">
        <property type="entry name" value="F64363"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496520.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1CJS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-219"/>
    </dbReference>
    <dbReference type="PDB" id="1I2A">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A=1-219"/>
    </dbReference>
    <dbReference type="PDB" id="1U63">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.40 A"/>
        <property type="chains" value="A/C=1-219"/>
    </dbReference>
    <dbReference type="PDB" id="4LQ4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A=1-211"/>
    </dbReference>
    <dbReference type="PDBsum" id="1CJS"/>
    <dbReference type="PDBsum" id="1I2A"/>
    <dbReference type="PDBsum" id="1U63"/>
    <dbReference type="PDBsum" id="4LQ4"/>
    <dbReference type="AlphaFoldDB" id="P54050"/>
    <dbReference type="SMR" id="P54050"/>
    <dbReference type="STRING" id="243232.MJ_0510"/>
    <dbReference type="EnsemblBacteria" id="AAB98500">
        <property type="protein sequence ID" value="AAB98500"/>
        <property type="gene ID" value="MJ_0510"/>
    </dbReference>
    <dbReference type="GeneID" id="1451372"/>
    <dbReference type="KEGG" id="mja:MJ_0510"/>
    <dbReference type="eggNOG" id="arCOG04289">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062853_4_0_2"/>
    <dbReference type="InParanoid" id="P54050"/>
    <dbReference type="OMA" id="GWTDVDV"/>
    <dbReference type="OrthoDB" id="70269at2157"/>
    <dbReference type="PhylomeDB" id="P54050"/>
    <dbReference type="EvolutionaryTrace" id="P54050"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_L1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_L1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_L1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_L1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_L1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_L1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023673">
        <property type="entry name" value="Ribosomal_L1_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="SSF56808"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01199">
        <property type="entry name" value="RIBOSOMAL_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0678">Repressor</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000125798" description="50S ribosomal protein L1">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="3"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="40"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="45"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="60"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="66"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="82"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="103"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="112"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="140"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="149"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="157"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="171"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="192"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="196"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="15659579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4LQ4"/>
        </source>
    </evidence>
    <sequence length="219" mass="24793" checksum="BEFF3E31B693F9EA" modified="2001-06-01" version="2">MDREALLQAVKEARELAKPRNFTQSFEFIATLKEIDMRKPENRIKTEVVLPHGRGKEAKIAVIGTGDLAKQAEELGLTVIRKEEIEELGKNKRKLRKIAKAHDFFIAQADLMPLIGRYMGVILGPRGKMPKPVPANANIKPLVERLKKTVVINTRDKPYFQVLVGNEKMTDEQIVDNIEAVLNVVAKKYEKGLYHIKDAYVKLTMGPAVKVKKEKAKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>P81232</accession>
    <name>Y21B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0210.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0210.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869706.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81232"/>
    <dbReference type="STRING" id="243232.MJ_0210.1"/>
    <dbReference type="DNASU" id="1451060"/>
    <dbReference type="EnsemblBacteria" id="AAB98206">
        <property type="protein sequence ID" value="AAB98206"/>
        <property type="gene ID" value="MJ_0210.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451060"/>
    <dbReference type="KEGG" id="mja:MJ_0210.1"/>
    <dbReference type="eggNOG" id="arCOG09658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582930_0_0_2"/>
    <dbReference type="OMA" id="YSWKEFK"/>
    <dbReference type="OrthoDB" id="136817at2157"/>
    <dbReference type="PhylomeDB" id="P81232"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106743" description="Uncharacterized protein MJ0210.1">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="172" mass="20084" checksum="510779FF2FE9817A" modified="1998-07-15" version="1">MFNKVAFMNIPMMDLIMIVIAIIITIGSFLFIAYLIFKYSKIKKQVKIIREVKINLPKMLKSNMIKNSFLIISLLCFYFGMLYIAGELVISHILFIAICWIVVFLYIIIKGETRGYICEEGLLVSGVLYSWKEFKDVKIEDNYIILTTPIHKIVIKKEKGVENILKNYLKRN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>P81302</accession>
    <name>Y04A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0047.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0047.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81302"/>
    <dbReference type="STRING" id="243232.MJ_0047.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98040">
        <property type="protein sequence ID" value="AAB98040"/>
        <property type="gene ID" value="MJ_0047.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0047.1"/>
    <dbReference type="HOGENOM" id="CLU_2230391_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106669" description="Uncharacterized protein MJ0047.1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <sequence length="105" mass="12400" checksum="645187616A9D9DFB" modified="1998-07-15" version="1">MKYNTHPFPSSIHSLRGLILIFSKYCFNAFNLIYFHPPRGLILTIIWEKGKVSITDKFPFRNGLILTNAKNLHYPQHNNWISIPKRSDFNRAIIHNIIYFITLNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>P81306</accession>
    <name>Y33A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0332.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0332.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869830.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81306"/>
    <dbReference type="SMR" id="P81306"/>
    <dbReference type="STRING" id="243232.MJ_0332.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98330">
        <property type="protein sequence ID" value="AAB98330"/>
        <property type="gene ID" value="MJ_0332.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451188"/>
    <dbReference type="KEGG" id="mja:MJ_0332.1"/>
    <dbReference type="eggNOG" id="arCOG09639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1976518_0_0_2"/>
    <dbReference type="OMA" id="LYQSMAL"/>
    <dbReference type="OrthoDB" id="140080at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106802" description="Uncharacterized protein MJ0332.1">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="132" mass="14972" checksum="6E11E1A58AB1641D" modified="1998-07-15" version="1">MMNGENMDKQTVIGFVVLFCVLELVFYLKKLYQSMALTLAVFGIFSLLFFLLYIPVLSKKAVPYVINYFKPPHQRVREIKVGSDETTDNSIIRLKEKAKTLHPDEGNRISGRSSNSFKDSASCIITIVDDSN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>P81315</accession>
    <name>Y85A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative transposase MJ0856.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0856.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81315"/>
    <dbReference type="STRING" id="243232.MJ_0856.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98861">
        <property type="protein sequence ID" value="AAB98861"/>
        <property type="gene ID" value="MJ_0856.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0856.1"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2177889_0_0_2"/>
    <dbReference type="InParanoid" id="P81315"/>
    <dbReference type="PhylomeDB" id="P81315"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0814">Transposable element</keyword>
    <keyword id="KW-0815">Transposition</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000107081" description="Putative transposase MJ0856.1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="109" mass="12230" checksum="23D38A215FA87354" modified="1998-07-15" version="1">MHNISAKKFLGYLKNKCLEFGVKVIEGNPAYTSIKCPNCGSRLSQLYKLADERALPSRLMYCFDCGFYADRDTVAVFNLIKRFTGLYPFSPKSNEPIAEGTVFPDEAMG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57555</accession>
    <name>Y090_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protease MJ0090</fullName>
            <ecNumber>3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0090</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U32 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64311">
        <property type="entry name" value="B64311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57555"/>
    <dbReference type="STRING" id="243232.MJ_0090"/>
    <dbReference type="DNASU" id="1450929"/>
    <dbReference type="EnsemblBacteria" id="AAB98071">
        <property type="protein sequence ID" value="AAB98071"/>
        <property type="gene ID" value="MJ_0090"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0090"/>
    <dbReference type="eggNOG" id="arCOG03202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011540_0_1_2"/>
    <dbReference type="InParanoid" id="Q57555"/>
    <dbReference type="OMA" id="YGGVSHF"/>
    <dbReference type="PhylomeDB" id="Q57555"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01276">
        <property type="entry name" value="PEPTIDASE_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000079186" description="Uncharacterized protease MJ0090">
        <location>
            <begin position="1"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="407" mass="46775" checksum="74FC8503D3A028DB" modified="1997-11-01" version="1">MMVMVELLSPANDLTCLKTAIDYGADAVYCGLKELNMRANAKNFTREELIEGIKYAHDNNKKVYLCTNTVVYENDLKKVEEILDFANSAEVDAVIVSDLGTMQLANELGLRVHASVQCNVTNSLTAKFYSKFAKRVILSRELTLNQIKEIRENLKKDKVDLELEGFVHGALCVAISGRCFLSSYLFGRHANCGDCLQPCRRKWKLINEHHDGTYEIVCEGKYLLSPKDLCMIEHIPELMEVFDSFKIEGRAKNADYVMRTTKIYREAIDSVLDGSYYDKLEYFKKELQKVYNRSYDTGFYFRDINKNHDFQYEIEGNASKYRKIEIGRVVNFYKKVSVAEIELWHDLKIGDTILIIGKTTGCVEEVVKSMQINHKDVEIAKKGERVGVKLNHLVREGDRVYLLKETI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q57559</accession>
    <name>Y094_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0094</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0094</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64311">
        <property type="entry name" value="F64311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57559"/>
    <dbReference type="SMR" id="Q57559"/>
    <dbReference type="STRING" id="243232.MJ_0094"/>
    <dbReference type="EnsemblBacteria" id="AAB98074">
        <property type="protein sequence ID" value="AAB98074"/>
        <property type="gene ID" value="MJ_0094"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0094"/>
    <dbReference type="eggNOG" id="arCOG03226">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_890295_0_0_2"/>
    <dbReference type="InParanoid" id="Q57559"/>
    <dbReference type="OMA" id="AKPNMIG"/>
    <dbReference type="PhylomeDB" id="Q57559"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011312">
        <property type="entry name" value="UCP019164_menthan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019164">
        <property type="entry name" value="UCP019164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03274">
        <property type="entry name" value="methan_mark_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106690" description="Uncharacterized protein MJ0094">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <sequence length="310" mass="35866" checksum="8D2F64D524CE2EB1" modified="1997-11-01" version="1">MVILMYEIVRYEGGVYKNNIFKEWIEDIGGFVIQEHVMQLDVYMTLAIPQNELENIKEEAKKYKGKIIETPLAGTEIAVVAPSLSRHHLPHTACDISEYLRRFGAKPNMIGLARGVGRDIAQLREKERRLIEEHDLAVYVMGNFEDCIKNKTHLFDVDIPVVVTGGPEKIDIPYPYVGNLGRRSHRLRHGEEIRALRKMVEVITELINERRRELSYDPPIVPPVVVKDEIEKQVEEVYSILSPMPIVTQLDGLRVKLDYDKYADKIREVKVKNYTLGDIADIKRSEMKNYILIKIKPKSEVEFEMHKDKA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q57564</accession>
    <name>ASST_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L-aspartate semialdehyde sulfurtransferase</fullName>
            <ecNumber evidence="1">2.8.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0100</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Acta Crystallogr. F" volume="64" first="936" last="941">
            <title>Purification, crystallization and preliminary X-ray diffraction analysis of the CBS-domain pair from the Methanococcus jannaschii protein MJ0100.</title>
            <authorList>
                <person name="Lucas M."/>
                <person name="Kortazar D."/>
                <person name="Astigarraga E."/>
                <person name="Fernandez J.A."/>
                <person name="Mato J.M."/>
                <person name="Martinez-Chantar M.L."/>
                <person name="Martinez-Cruz L.A."/>
            </authorList>
            <dbReference type="PubMed" id="18931440"/>
            <dbReference type="DOI" id="10.1107/s1744309108027930"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference evidence="6 7 8" key="3">
        <citation type="journal article" date="2010" name="J. Mol. Biol." volume="396" first="800" last="820">
            <title>Binding of S-methyl-5'-thioadenosine and S-adenosyl-L-methionine to protein MJ0100 triggers an open-to-closed conformational change in its CBS motif pair.</title>
            <authorList>
                <person name="Lucas M."/>
                <person name="Encinar J.A."/>
                <person name="Arribas E.A."/>
                <person name="Oyenarte I."/>
                <person name="Garcia I.G."/>
                <person name="Kortazar D."/>
                <person name="Fernandez J.A."/>
                <person name="Mato J.M."/>
                <person name="Martinez-Chantar M.L."/>
                <person name="Martinez-Cruz L.A."/>
            </authorList>
            <dbReference type="PubMed" id="20026078"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2009.12.012"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 388-509 IN COMPLEXES WITH S-ADENOSYL-L-METHIONINE AND S-METHYL-5'-THIOADENOSINE</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Required for O-acetylhomoserine sulfhydrylase (OAHS)-independent homocysteine (Hcy) biosynthesis. Together with MJ0099, catalyzes the condensation of sulfide with aspartate semialdehyde to generate homocysteine. Likely functions through persulfide intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3 H(+) + hydrogen sulfide + L-aspartate 4-semialdehyde + reduced 2[4Fe-4S]-[ferredoxin] = H2O + L-homocysteine + oxidized 2[4Fe-4S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:58412"/>
            <dbReference type="Rhea" id="RHEA-COMP:10002"/>
            <dbReference type="Rhea" id="RHEA-COMP:10004"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="2.8.1.16"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">The ligand-induced conformational reorganization of the protein could be an important regulatory mechanism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3 4">Homodimer (PubMed:18931440, PubMed:20026078). May form a complex with MJ0099.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The C-terminal cystathionine beta-synthase (CBS) domains can bind different numbers of S-adenosyl-L-methionine (SAM) and S-methyl-5'-thioadenosine (MTA) ligands. Binding of SAM and MTA triggers a drastic conformational change in the protein, which evolves progressively from an open form in the absence of ligands to a closed form when the four sites are fully occupied.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the L-aspartate semialdehyde sulfurtransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.16" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64312">
        <property type="entry name" value="D64312"/>
    </dbReference>
    <dbReference type="PDB" id="3KPB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A/B/C/D=388-509"/>
    </dbReference>
    <dbReference type="PDB" id="3KPC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.79 A"/>
        <property type="chains" value="A=386-509"/>
    </dbReference>
    <dbReference type="PDB" id="3KPD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.91 A"/>
        <property type="chains" value="A/B/C/D=388-509"/>
    </dbReference>
    <dbReference type="PDBsum" id="3KPB"/>
    <dbReference type="PDBsum" id="3KPC"/>
    <dbReference type="PDBsum" id="3KPD"/>
    <dbReference type="AlphaFoldDB" id="Q57564"/>
    <dbReference type="SMR" id="Q57564"/>
    <dbReference type="STRING" id="243232.MJ_0100"/>
    <dbReference type="EnsemblBacteria" id="AAB98080">
        <property type="protein sequence ID" value="AAB98080"/>
        <property type="gene ID" value="MJ_0100"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0100"/>
    <dbReference type="eggNOG" id="arCOG00620">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043239_0_0_2"/>
    <dbReference type="InParanoid" id="Q57564"/>
    <dbReference type="OMA" id="WVRGCYF"/>
    <dbReference type="PhylomeDB" id="Q57564"/>
    <dbReference type="BRENDA" id="2.8.1.16">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57564"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016426">
        <property type="entry name" value="MA1821-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004698">
        <property type="entry name" value="UCP004698_CBS_MJ0100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-0486">Methionine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106693" description="L-aspartate semialdehyde sulfurtransferase">
        <location>
            <begin position="1"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="2">
        <location>
            <begin position="394"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="2">
        <location>
            <begin position="455"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="399"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="421"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="456"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="460"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="479"/>
            <end position="482"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="497"/>
            <end position="500"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="390"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="394"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="407"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="422"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="429"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="437"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="451"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="455"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="468"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="481"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="strand" evidence="9">
        <location>
            <begin position="491"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="helix" evidence="9">
        <location>
            <begin position="498"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8TPT4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18931440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="20026078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="3KPB"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
        <source>
            <dbReference type="PDB" id="3KPC"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="8">
        <source>
            <dbReference type="PDB" id="3KPD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="3KPB"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="3KPD"/>
        </source>
    </evidence>
    <sequence length="509" mass="56458" checksum="81417970CD74F38F" modified="1997-11-01" version="1">MIMKTIKEINEKIKKGEAVVVTAEEMIKIVEEEGAKRAADYVDVVTTGTFGAMCSSGVFINFGHSDPPIKMLRIYLNNVEAYGGLAAVDAYIGAAQPNEDPDVDIDYGGAHVIEDLVRGKEVELYAEGYTTDCYPRKEVNVRITLDDVNQAIMVNPRNCYQTYAAATNSREEKIYTYMGILLPEYNNVHYSGAGQLNPLQNDYNPETKSFNTIGIGTRIFLGGGIGYVIGEGTQHNPPFGTLMVKGDLKQMNPKFIRAATMPRYGSTLYVGIGIPIPVLNEKIAERCAIRDEDIEVPIYDYGFPRRDRPLIAKTNYKVLRSGKITLNVNIDGKDVEKTVKTGSVSSYKMAREVAETLKQWILDGKFLLTERVDTLGRAENKPMKSPITLVKDILSKPPITAHSNISIMEAAKILIKHNINHLPIVDEHGKLVGIITSWDIAKALAQNKKTIEEIMTRNVITAHEDEPVDHVAIKMSKYNISGVPVVDDYRRVVGIVTSEDISRLFGGKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57567</accession>
    <name>Y103_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0103</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0103</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the radical SAM superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64312">
        <property type="entry name" value="G64312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869595.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57567"/>
    <dbReference type="SMR" id="Q57567"/>
    <dbReference type="STRING" id="243232.MJ_0103"/>
    <dbReference type="EnsemblBacteria" id="AAB98083">
        <property type="protein sequence ID" value="AAB98083"/>
        <property type="gene ID" value="MJ_0103"/>
    </dbReference>
    <dbReference type="GeneID" id="1450944"/>
    <dbReference type="KEGG" id="mja:MJ_0103"/>
    <dbReference type="eggNOG" id="arCOG00951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048071_0_0_2"/>
    <dbReference type="InParanoid" id="Q57567"/>
    <dbReference type="OMA" id="CIFCSVD"/>
    <dbReference type="OrthoDB" id="68339at2157"/>
    <dbReference type="PhylomeDB" id="Q57567"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040088">
        <property type="entry name" value="MJ0103-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01110">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000171931" description="Uncharacterized protein MJ0103">
        <location>
            <begin position="1"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="104"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="2">
        <location>
            <begin position="370"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="171"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="236"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="433" mass="49866" checksum="F82576531DF12142" modified="1997-11-01" version="1">MVCLDLSQYRMITDVKNKDNTLILEINKIYEIEVEIPYEEVEIDGSIIKINAHPKRAENIKVGILNLISYSIANNLKSKITKRKTIYINEPIPLIGHTAFGLIERGRNIIQVRGHCGCNLNCIFCSVDEGEFSKTRKNDYYVDLEYLIENYKKIVDFKENKFIEAHLDGQGEPSLYYPLVDLVQELAEINKKGNGIVSMQTNGTVLNYKLIDELEEAGLHRINLSINALDEKMAKMLSGRRDYNIKKILDIAEYIKNSKIHLLIAPLLLPNINDEEFKRVIEYAVDLEQKNPQNIINPLTGKKDPILGCQLCRVYQLGRRPKKMKVWDFEKFYYLLGKYELEYKKKGIEVKLITSPKDFGTHKRKRLPYPFKVGEVTKVKVVLDGRVKGEVLGVAKDRVIQIINCNNEQNLIGKTVKVRILRNKDNIIVAELV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q57585</accession>
    <name>Y121_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ0121</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0121</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64315">
        <property type="entry name" value="A64315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869614.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57585"/>
    <dbReference type="SMR" id="Q57585"/>
    <dbReference type="STRING" id="243232.MJ_0121"/>
    <dbReference type="EnsemblBacteria" id="AAB98102">
        <property type="protein sequence ID" value="AAB98102"/>
        <property type="gene ID" value="MJ_0121"/>
    </dbReference>
    <dbReference type="GeneID" id="1450964"/>
    <dbReference type="KEGG" id="mja:MJ_0121"/>
    <dbReference type="eggNOG" id="arCOG03228">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072513_0_0_2"/>
    <dbReference type="InParanoid" id="Q57585"/>
    <dbReference type="OMA" id="LADIECM"/>
    <dbReference type="OrthoDB" id="45551at2157"/>
    <dbReference type="PhylomeDB" id="Q57585"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093218" description="Uncharacterized ABC transporter ATP-binding protein MJ0121">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="261" mass="29262" checksum="6C26F1CBEB2FE5A2" modified="1997-11-01" version="1">MEIKEITIIGGYDKNGNPEPVREVTIKRGEIVGVVGPTGSGKSNLISDIEQLAQGDTISKRRILVNGEVPPIEMRRDPKKRRIAQLSQNMNFLADMTVEEFILMHAKSRGVYRENIVDEVIELANRLTGEPIKKDYNLTILSGGQSRSLMVADVAVISDSPIVLIDEIENAGIKKHEALELLAGYGKIVLVITHDPVLALMTDRRIVMRNGGMQKIIETTEEEKEISRKINEVDNWLLSLREKIRFGERLTHEDISLMVKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q57595</accession>
    <name>Y131_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0131</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0131</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64316">
        <property type="entry name" value="C64316"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869624.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57595"/>
    <dbReference type="STRING" id="243232.MJ_0131"/>
    <dbReference type="EnsemblBacteria" id="AAB98119">
        <property type="protein sequence ID" value="AAB98119"/>
        <property type="gene ID" value="MJ_0131"/>
    </dbReference>
    <dbReference type="GeneID" id="1450972"/>
    <dbReference type="KEGG" id="mja:MJ_0131"/>
    <dbReference type="eggNOG" id="arCOG06571">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257380_0_0_2"/>
    <dbReference type="OMA" id="WIIVNTI"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106709" description="Uncharacterized protein MJ0131">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="103" mass="11861" checksum="F028E0050D09F9A6" modified="1997-11-01" version="1">MPGVIFWTIFIIQFMIWVFLSTTKIGRVIAFIWGTMPFLALYLKYTGYFPTIFENPDVNLFANLLSNFVVEWSYLVVQTTVPSWIGLLFGLKLSGNNDTPQII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q57630</accession>
    <name>DTDA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="1">3.1.1.96</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>D-tyrosyl-tRNA(Tyr) deacylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dtdA</name>
        <name type="ordered locus">MJ0166</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a D-aminoacyl-tRNA + H2O = a D-alpha-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-tRNA(Ala) + H2O = glycine + H(+) + tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtdA deacylase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64320">
        <property type="entry name" value="G64320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869661.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57630"/>
    <dbReference type="SMR" id="Q57630"/>
    <dbReference type="STRING" id="243232.MJ_0166"/>
    <dbReference type="EnsemblBacteria" id="AAB98148">
        <property type="protein sequence ID" value="AAB98148"/>
        <property type="gene ID" value="MJ_0166"/>
    </dbReference>
    <dbReference type="GeneID" id="1451013"/>
    <dbReference type="KEGG" id="mja:MJ_0166"/>
    <dbReference type="eggNOG" id="arCOG01616">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056464_1_0_2"/>
    <dbReference type="InParanoid" id="Q57630"/>
    <dbReference type="OMA" id="CYEATHH"/>
    <dbReference type="OrthoDB" id="84039at2157"/>
    <dbReference type="PhylomeDB" id="Q57630"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="PTHR34667"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000158966" description="D-aminoacyl-tRNA deacylase">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <sequence length="255" mass="29072" checksum="6428DF7AEC802CE4" modified="1996-11-01" version="1">MKFLLIASNKDLASKNIANHIKEYFDVFETDKELLSLTAEDLEYADYYIFLSKHKSIANKPSLTVHTPGNLTEDNTFGGNPKEVCPCDAVLNTLLLKNIYKNYKTYYEDGKIGEFDVSFEVVHHSPTGLKAPTVFVEIGSSEKEWILKEAGEIIAKSVLETIDAMKSKNYDKKVRAIGFGGGHYAPKFTKLALEDKYYFGYLVPKYASVSEDVLNQLISKMEVDKALIDWKGCRGDDKRRYIEFFENNGIEWERV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57631</accession>
    <name>MOAB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable molybdopterin adenylyltransferase</fullName>
            <shortName>MPT adenylyltransferase</shortName>
            <ecNumber>2.7.7.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">moaB</name>
        <name type="ordered locus">MJ0167</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the adenylation of molybdopterin as part of the biosynthesis of the molybdenum-cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H(+) + molybdopterin = adenylyl-molybdopterin + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:31331"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58698"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="EC" id="2.7.7.75"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MoaB/Mog family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.75"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64320">
        <property type="entry name" value="H64320"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869662.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57631"/>
    <dbReference type="SMR" id="Q57631"/>
    <dbReference type="STRING" id="243232.MJ_0167"/>
    <dbReference type="EnsemblBacteria" id="AAB98149">
        <property type="protein sequence ID" value="AAB98149"/>
        <property type="gene ID" value="MJ_0167"/>
    </dbReference>
    <dbReference type="GeneID" id="1451014"/>
    <dbReference type="KEGG" id="mja:MJ_0167"/>
    <dbReference type="eggNOG" id="arCOG00214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077358_2_3_2"/>
    <dbReference type="InParanoid" id="Q57631"/>
    <dbReference type="OMA" id="TGWDGIL"/>
    <dbReference type="OrthoDB" id="108340at2157"/>
    <dbReference type="PhylomeDB" id="Q57631"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061598">
        <property type="term" value="F:molybdopterin adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="PTHR43232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="SSF53218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000170981" description="Probable molybdopterin adenylyltransferase">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="163" mass="18247" checksum="5B7FA49A5A223435" modified="1996-11-01" version="1">MHKRIKNIKYAVVTVSDSRYNDLIKGKEVDDKSGKLLKKELNAKVYTIIPDNKNMIKGIVEHIVEFFDVDCIVFTGGTGIAERDVTVEALKEIIEKELDGFKIIFQKLSYEEVGFSAMLSRAMAGIYKGKIIYALPGSVNACRTALKIIKEETGHILGHLREG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q57637</accession>
    <name>Y173_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative transcriptional regulatory protein MJ0173</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0173</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative transcriptional regulator.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Tfx family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98158" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98158.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64321">
        <property type="entry name" value="F64321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496415.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57637"/>
    <dbReference type="SMR" id="Q57637"/>
    <dbReference type="STRING" id="243232.MJ_0173"/>
    <dbReference type="EnsemblBacteria" id="AAB98158">
        <property type="protein sequence ID" value="AAB98158"/>
        <property type="gene ID" value="MJ_0173"/>
    </dbReference>
    <dbReference type="GeneID" id="1451020"/>
    <dbReference type="KEGG" id="mja:MJ_0173"/>
    <dbReference type="eggNOG" id="arCOG04554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125807_0_1_2"/>
    <dbReference type="InParanoid" id="Q57637"/>
    <dbReference type="OMA" id="VKVPYST"/>
    <dbReference type="OrthoDB" id="96651at2157"/>
    <dbReference type="PhylomeDB" id="Q57637"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00620">
        <property type="entry name" value="HTH_type_Tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007630">
        <property type="entry name" value="RNA_pol_sigma70_r4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029291">
        <property type="entry name" value="Tfx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004645">
        <property type="entry name" value="Tfx_DNA-bd_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018384">
        <property type="entry name" value="Tfx_DNA-bd_euryarc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036657">
        <property type="entry name" value="Tfx_DNA-bd_sf_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04545">
        <property type="entry name" value="Sigma70_r4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14601">
        <property type="entry name" value="TFX_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004932">
        <property type="entry name" value="DNA_bind_Tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89915">
        <property type="entry name" value="SSF89915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00721">
        <property type="entry name" value="tfx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000144170" description="Putative transcriptional regulatory protein MJ0173">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="137" mass="15695" checksum="CDAF26114D7FAB79" modified="2003-02-28" version="2">MVEDSFLTDTQIKVLKLRKKGLTQEEIAKMLGTSRANISMIEKRAKENIKKAYNTIKIYNRIMAPLSIEIEEGTDVLEIPDIVFKKADEEGIKVKYNTLELIELIKENASEFIEKRTVKKKFKIYILENGDLDVGGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q57690</accession>
    <name>TRPG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Anthranilate synthase component 2</fullName>
            <shortName>AS</shortName>
            <shortName>ASII</shortName>
            <ecNumber>4.1.3.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Anthranilate synthase, GATase component</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Anthranilate synthase, glutamine amidotransferase component</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">trpG</name>
        <name type="ordered locus">MJ0238</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>chorismate + L-glutamine = anthranilate + H(+) + L-glutamate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64329">
        <property type="entry name" value="G64329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869736.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57690"/>
    <dbReference type="SMR" id="Q57690"/>
    <dbReference type="STRING" id="243232.MJ_0238"/>
    <dbReference type="MEROPS" id="C26.959"/>
    <dbReference type="EnsemblBacteria" id="AAB98224">
        <property type="protein sequence ID" value="AAB98224"/>
        <property type="gene ID" value="MJ_0238"/>
    </dbReference>
    <dbReference type="GeneID" id="1451092"/>
    <dbReference type="KEGG" id="mja:MJ_0238"/>
    <dbReference type="eggNOG" id="arCOG00086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_1_2_2"/>
    <dbReference type="InParanoid" id="Q57690"/>
    <dbReference type="OMA" id="TEHGHAM"/>
    <dbReference type="OrthoDB" id="100703at2157"/>
    <dbReference type="PhylomeDB" id="Q57690"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000056888" description="Anthranilate synthase component 2">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="3">
        <location>
            <begin position="5"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; for GATase activity" evidence="3">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="3">
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="3">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="55"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="130"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00605"/>
        </source>
    </evidence>
    <sequence length="197" mass="22061" checksum="982A3475C4155B7D" modified="1996-11-01" version="1">MEVKKVLVIDNIDSFVWNLVQYVGTLGYKVKLVDNKITLDEIKKINPDRIIISPGPKTPKEAGNCIKIIQEVDIPILGVCLGHQCIVEAFGGEVGRAKRVMHGKASLINHDGEGIFKDIPNPFYGGRYHSLIAKEVPKELKITAKSLDDNYIMGVRHKKLPIEGVQFHPESILTESDNLKFPDLGLKLIKNFVESEY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57696</accession>
    <name>CHMU_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Chorismate mutase</fullName>
            <shortName>CM</shortName>
            <ecNumber>5.4.99.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Monofunctional chorismate mutase AroQ(f)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">aroQ</name>
        <name type="synonym">aroQ(f)</name>
        <name type="ordered locus">MJ0246</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Biochemistry" volume="37" first="10062" last="10073">
            <title>A small, thermostable, and monofunctional chorismate mutase from the archaeon Methanococcus jannaschii.</title>
            <authorList>
                <person name="MacBeath G."/>
                <person name="Kast P."/>
                <person name="Hilvert D."/>
            </authorList>
            <dbReference type="PubMed" id="9665711"/>
            <dbReference type="DOI" id="10.1021/bi980449t"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>GENE NAME</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2007" name="Nat. Struct. Mol. Biol." volume="14" first="1202" last="1206">
            <title>Structure and dynamics of a molten globular enzyme.</title>
            <authorList>
                <person name="Pervushin K."/>
                <person name="Vamvaca K."/>
                <person name="Vogeli B."/>
                <person name="Hilvert D."/>
            </authorList>
            <dbReference type="PubMed" id="17994104"/>
            <dbReference type="DOI" id="10.1038/nsmb1325"/>
        </citation>
        <scope>STRUCTURE BY NMR OF 1-93</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of chorismate into prephenate via a Claisen rearrangement.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>chorismate = prephenate</text>
            <dbReference type="Rhea" id="RHEA:13897"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29934"/>
            <dbReference type="EC" id="5.4.99.5"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">41 uM for chorismate (at 30 degrees Celsius)</KM>
            <text>kcat is 5.7 sec(-1) (at 30 degrees Celsius).</text>
        </kinetics>
        <phDependence>
            <text evidence="3">Activity is independent of pH in the range of pH 5-9.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Thermostable. The midpoint for its thermal unfolding transition (Tm) is 88 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64330">
        <property type="entry name" value="G64330"/>
    </dbReference>
    <dbReference type="PDB" id="2GTV">
        <property type="method" value="NMR"/>
        <property type="chains" value="X=1-93"/>
    </dbReference>
    <dbReference type="PDBsum" id="2GTV"/>
    <dbReference type="AlphaFoldDB" id="Q57696"/>
    <dbReference type="SMR" id="Q57696"/>
    <dbReference type="STRING" id="243232.MJ_0246"/>
    <dbReference type="EnsemblBacteria" id="AAB98234">
        <property type="protein sequence ID" value="AAB98234"/>
        <property type="gene ID" value="MJ_0246"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0246"/>
    <dbReference type="eggNOG" id="arCOG02098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131518_4_0_2"/>
    <dbReference type="InParanoid" id="Q57696"/>
    <dbReference type="OMA" id="MDYNKEV"/>
    <dbReference type="PhylomeDB" id="Q57696"/>
    <dbReference type="SABIO-RK" id="Q57696"/>
    <dbReference type="UniPathway" id="UPA00120">
        <property type="reaction ID" value="UER00203"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57696"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009697">
        <property type="term" value="P:salicylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010950">
        <property type="entry name" value="Chorismate_mutase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="SSF48600"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01791">
        <property type="entry name" value="CM_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119202" description="Chorismate mutase">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate mutase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="4"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="23"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="69"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="9665711"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2GTV"/>
        </source>
    </evidence>
    <sequence length="99" mass="11782" checksum="9759018D0A07E76C" modified="1996-11-01" version="1">MIEKLAEIRKKIDEIDNKILKLIAERNSLAKDVAEIKNQLGIPINDPEREKYIYDRIRKLCKEHNVDENIGIKIFQILIEHNKALQKQYLEETQNKNKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q57731</accession>
    <name>APBC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0283</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="J. Bacteriol." volume="191" first="1490" last="1497">
            <title>Archaeal ApbC/Nbp35 homologs function as iron-sulfur cluster carrier proteins.</title>
            <authorList>
                <person name="Boyd J.M."/>
                <person name="Drevland R.M."/>
                <person name="Downs D.M."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="19114487"/>
            <dbReference type="DOI" id="10.1128/JB.01469-08"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64335">
        <property type="entry name" value="D64335"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869781.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57731"/>
    <dbReference type="SMR" id="Q57731"/>
    <dbReference type="STRING" id="243232.MJ_0283"/>
    <dbReference type="EnsemblBacteria" id="AAB98271">
        <property type="protein sequence ID" value="AAB98271"/>
        <property type="gene ID" value="MJ_0283"/>
    </dbReference>
    <dbReference type="GeneID" id="1451138"/>
    <dbReference type="KEGG" id="mja:MJ_0283"/>
    <dbReference type="eggNOG" id="arCOG00585">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_1_2"/>
    <dbReference type="InParanoid" id="Q57731"/>
    <dbReference type="OMA" id="NMAYFTP"/>
    <dbReference type="OrthoDB" id="32313at2157"/>
    <dbReference type="PhylomeDB" id="Q57731"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="PTHR23264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184953" description="Iron-sulfur cluster carrier protein">
        <location>
            <begin position="1"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19114487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="19114487"/>
        </source>
    </evidence>
    <sequence length="290" mass="31243" checksum="D5C0E3A15DB728EF" modified="1996-11-01" version="1">MAECDGKCDTCPSKNTCPDTKKLLAQQDAKIRENMSKIKHKIVILSGKGGVGKSTVTVNLAAALNLMGKKVGVLDADIHGPNIPKMLGVENTQPMAGPAGIFPIVTKDGIKTMSIGYLLPDDKTPVIWRGPKVSGAIRQFLSDVVWGELDYLLIDTPPGTGDEQLTIMQSIPDIDGAIIVTTPEEVSVLDVKKSIMMAKMLNIPIIGIIENMSGFVCPYCNKVVDIFGRGGGEKAAKELGVEFLGRIPLDIKAREASDKGIPMVLLDCKASEEFKKIVKRIVEKVEGKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57740</accession>
    <name>Y292_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0292</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0292</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64336">
        <property type="entry name" value="E64336"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57740"/>
    <dbReference type="SMR" id="Q57740"/>
    <dbReference type="STRING" id="243232.MJ_0292"/>
    <dbReference type="EnsemblBacteria" id="AAB98277">
        <property type="protein sequence ID" value="AAB98277"/>
        <property type="gene ID" value="MJ_0292"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0292"/>
    <dbReference type="eggNOG" id="arCOG04877">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_171540_0_0_2"/>
    <dbReference type="OMA" id="GVIDCAC"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106778" description="Uncharacterized protein MJ0292">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="93" mass="10483" checksum="B2A8CC8115AC160C" modified="1996-11-01" version="1">MVMLMEQFIGIVKDILVLIASFGILLASYRLWIEKDRKNIIYARIHILGVIDCACFLIFIALGETLLAFVYLILAPFLAHAIAHAAYNDNLSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="150" xmlns="http://uniprot.org/uniprot">
    <accession>Q57834</accession>
    <name>SYY_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tyrosine--tRNA ligase</fullName>
            <ecNumber>6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Tyrosyl-tRNA synthetase</fullName>
            <shortName>TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tyrS</name>
        <name type="ordered locus">MJ0389</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Biol. Chem." volume="274" first="35601" last="35606">
            <title>Major anticodon-binding region missing from an archaebacterial tRNA synthetase.</title>
            <authorList>
                <person name="Steer B.A."/>
                <person name="Schimmel P."/>
            </authorList>
            <dbReference type="PubMed" id="10585437"/>
            <dbReference type="DOI" id="10.1074/jbc.274.50.35601"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>KINETIC PARAMETERS</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1999" name="Proc. Natl. Acad. Sci. U.S.A." volume="96" first="13644" last="13649">
            <title>Domain-domain communication in a miniature archaebacterial tRNA synthetase.</title>
            <authorList>
                <person name="Steer B.A."/>
                <person name="Schimmel P."/>
            </authorList>
            <dbReference type="PubMed" id="10570126"/>
            <dbReference type="DOI" id="10.1073/pnas.96.24.13644"/>
        </citation>
        <scope>MUTAGENESIS OF ASP-286 AND LYS-288</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2003" name="Nat. Struct. Biol." volume="10" first="425" last="432">
            <title>Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion.</title>
            <authorList>
                <person name="Kobayashi T."/>
                <person name="Nureki O."/>
                <person name="Ishitani R."/>
                <person name="Yaremchuk A."/>
                <person name="Tukalo M."/>
                <person name="Cusack S."/>
                <person name="Sakamoto K."/>
                <person name="Yokoyama S."/>
            </authorList>
            <dbReference type="PubMed" id="12754495"/>
            <dbReference type="DOI" id="10.1038/nsb934"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH TRNA(TYR) AND TYROSINE</scope>
        <scope>MUTAGENESIS OF ASP-286</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2005" name="Protein Sci." volume="14" first="1340" last="1349">
            <title>Crystal structures of apo wild-type M. jannaschii tyrosyl-tRNA synthetase (TyrRS) and an engineered TyrRS specific for O-methyl-L-tyrosine.</title>
            <authorList>
                <person name="Zhang Y."/>
                <person name="Wang L."/>
                <person name="Schultz P.G."/>
                <person name="Wilson I.A."/>
            </authorList>
            <dbReference type="PubMed" id="15840835"/>
            <dbReference type="DOI" id="10.1110/ps.041239305"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.66 ANGSTROMS) OF WILD-TYPE AND O-METHYL-TYROSINE-SPECIFIC MUTANT APOENZYME</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + H(+) + L-tyrosyl-tRNA(Tyr)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">15 uM for tRNA(Tyr) (at 45 degrees Celsius)</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="3 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64348">
        <property type="entry name" value="E64348"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869888.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1J1U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="A=1-306"/>
    </dbReference>
    <dbReference type="PDB" id="1U7D">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.65 A"/>
        <property type="chains" value="A/B=1-306"/>
    </dbReference>
    <dbReference type="PDB" id="1U7X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A/B=1-306"/>
    </dbReference>
    <dbReference type="PDB" id="1ZH0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A=1-306"/>
    </dbReference>
    <dbReference type="PDB" id="1ZH6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=1-306"/>
    </dbReference>
    <dbReference type="PDB" id="2AG6">
        <property type="method" value="X-ray"/>
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    </evidence>
    <sequence length="306" mass="35049" checksum="1887760ABAF2DD5E" modified="1997-11-01" version="1">MDEFEMIKRNTSEIISEEELREVLKKDEKSAYIGFEPSGKIHLGHYLQIKKMIDLQNAGFDIIILLADLHAYLNQKGELDEIRKIGDYNKKVFEAMGLKAKYVYGSEFQLDKDYTLNVYRLALKTTLKRARRSMELIAREDENPKVAEVIYPIMQVNDIHYLGVDVAVGGMEQRKIHMLARELLPKKVVCIHNPVLTGLDGEGKMSSSKGNFIAVDDSPEEIRAKIKKAYCPAGVVEGNPIMEIAKYFLEYPLTIKRPEKFGGDLTVNSYEELESLFKNKELHPMDLKNAVAEELIKILEPIRKRL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57845</accession>
    <name>Y402_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0402</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0402</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64350">
        <property type="entry name" value="B64350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869901.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57845"/>
    <dbReference type="SMR" id="Q57845"/>
    <dbReference type="STRING" id="243232.MJ_0402"/>
    <dbReference type="EnsemblBacteria" id="AAB98395">
        <property type="protein sequence ID" value="AAB98395"/>
        <property type="gene ID" value="MJ_0402"/>
    </dbReference>
    <dbReference type="GeneID" id="1451262"/>
    <dbReference type="KEGG" id="mja:MJ_0402"/>
    <dbReference type="eggNOG" id="arCOG08261">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181980_0_0_2"/>
    <dbReference type="OMA" id="HAWDLYR"/>
    <dbReference type="OrthoDB" id="126390at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106854" description="Uncharacterized protein MJ0402">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <sequence length="106" mass="12600" checksum="6398888ABD46CFF3" modified="1996-11-01" version="1">MFGWGRGWFGRGRGFWRYFPVSTVGGRYRYVGPCRCGLGPHAFYVDEKTGALVHAWDLYRGYVPGYAEVDERRYLEETIKELEEEKRMLEEELARIKKRLDELKKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q57853</accession>
    <name>Y410_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0410</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0410</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64351">
        <property type="entry name" value="B64351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869909.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57853"/>
    <dbReference type="SMR" id="Q57853"/>
    <dbReference type="STRING" id="243232.MJ_0410"/>
    <dbReference type="EnsemblBacteria" id="AAB98399">
        <property type="protein sequence ID" value="AAB98399"/>
        <property type="gene ID" value="MJ_0410"/>
    </dbReference>
    <dbReference type="GeneID" id="1451270"/>
    <dbReference type="KEGG" id="mja:MJ_0410"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="InParanoid" id="Q57853"/>
    <dbReference type="OMA" id="DIDCIGV"/>
    <dbReference type="OrthoDB" id="72034at2157"/>
    <dbReference type="PhylomeDB" id="Q57853"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106859" description="Uncharacterized ATP-binding protein MJ0410">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="264" mass="29512" checksum="BC6D63DAF061B1EB" modified="1996-11-01" version="1">MKIGFYNIQGGTGKTTVAANFAYILSQSVKTILIDCDIYGGTTAVLFGLEDKEHNLNTYLAGDSAIEDIIYHYDDLAIIHTDVSSKVFGYKSDLNRFETLVKELEEEYDVIIYDFPPNITEDNPLIGYVGEFELVNKVVVVGEDSIPSIVNSLKTIELITDLGIGLTGIIVNKYRGLTDISEIIDDVIGVLPYDQNVERQWVESTPIVKIKTKFTKEMTALANEIASIYLEKDLASLRALRLAKAISELTEVEKSEKDFEDYLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q57859</accession>
    <name>Y416_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Double zinc ribbon protein MJ0416</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0416</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64351">
        <property type="entry name" value="H64351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869915.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57859"/>
    <dbReference type="STRING" id="243232.MJ_0416"/>
    <dbReference type="EnsemblBacteria" id="AAB98407">
        <property type="protein sequence ID" value="AAB98407"/>
        <property type="gene ID" value="MJ_0416"/>
    </dbReference>
    <dbReference type="GeneID" id="1451276"/>
    <dbReference type="KEGG" id="mja:MJ_0416"/>
    <dbReference type="eggNOG" id="arCOG01917">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431668_0_0_2"/>
    <dbReference type="OMA" id="PYCGYRL"/>
    <dbReference type="OrthoDB" id="65836at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000106862" description="Double zinc ribbon protein MJ0416">
        <location>
            <begin position="1"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="DZANK-type" evidence="1">
        <location>
            <begin position="134"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="190" mass="21889" checksum="D7E2AB55B759606F" modified="1996-11-01" version="1">MVRVVPLTDEEKMSIVSGLRSSVPATKLVTLRKLQEIAEVRPEAILYLDTYDKVTLNEIITLLNQIIEYDPDEILRREAMITLEKVKKALGTKFSTFVPLCNSCKSPIDLGWTYCTNCGAEIKNMTFEEEVERCPNCNNYISDSWKYCAHCGAKLKEEEEEVLRCPNCKRPVQPEWIVCPYCGYRLKRKP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q57867</accession>
    <name>Y424_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0424</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0424</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64352">
        <property type="entry name" value="H64352"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57867"/>
    <dbReference type="SMR" id="Q57867"/>
    <dbReference type="STRING" id="243232.MJ_0424"/>
    <dbReference type="EnsemblBacteria" id="AAB98418">
        <property type="protein sequence ID" value="AAB98418"/>
        <property type="gene ID" value="MJ_0424"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0424"/>
    <dbReference type="eggNOG" id="arCOG03166">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257381_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106869" description="Uncharacterized protein MJ0424">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <sequence length="103" mass="12056" checksum="4C7B826FE537D176" modified="1996-11-01" version="1">MLTNDSMNENLNELKRVFSSLTGKEYFKNLDVGLVDLFRYLRDEIKDEKVVIALDEFQYLMQLNRGVLSIFQKIWDGILADTKVFLIICGSSCVSKHGRFRDW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q57871</accession>
    <name>WECC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UDP-N-acetyl-D-mannosamine dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.336</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>UDP-ManNAc 6-dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">wecC</name>
        <name type="ordered locus">MJ0428</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the four-electron oxidation of UDP-N-acetyl-D-mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N-acetylmannosaminuronic acid (UDP-ManNAcA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H2O + 2 NAD(+) + UDP-N-acetyl-alpha-D-mannosamine = 3 H(+) + 2 NADH + UDP-N-acetyl-alpha-D-mannosaminouronate</text>
            <dbReference type="Rhea" id="RHEA:25780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:68623"/>
            <dbReference type="ChEBI" id="CHEBI:70731"/>
            <dbReference type="EC" id="1.1.1.336"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.336" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64353">
        <property type="entry name" value="D64353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869927.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57871"/>
    <dbReference type="SMR" id="Q57871"/>
    <dbReference type="STRING" id="243232.MJ_0428"/>
    <dbReference type="EnsemblBacteria" id="AAB98413">
        <property type="protein sequence ID" value="AAB98413"/>
        <property type="gene ID" value="MJ_0428"/>
    </dbReference>
    <dbReference type="GeneID" id="1451288"/>
    <dbReference type="KEGG" id="mja:MJ_0428"/>
    <dbReference type="eggNOG" id="arCOG00252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023810_3_2_2"/>
    <dbReference type="InParanoid" id="Q57871"/>
    <dbReference type="OMA" id="GHCLPID"/>
    <dbReference type="OrthoDB" id="41144at2157"/>
    <dbReference type="PhylomeDB" id="Q57871"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089714">
        <property type="term" value="F:UDP-N-acetyl-D-mannosamine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000074083" description="UDP-N-acetyl-D-mannosamine dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate" evidence="1">
        <location>
            <begin position="157"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate" evidence="1">
        <location>
            <begin position="213"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate; shared with dimeric partner" evidence="1">
        <location>
            <begin position="247"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Substrate" evidence="1">
        <location>
            <begin position="317"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O59284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q6LZC3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3"/>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="427" mass="47749" checksum="0F0EAAFC17F8BB76" modified="1996-11-01" version="1">MTKVEKNGIGKRICVIGLGYIGLPTASMLAIQGFDVIGVDINEKRVKEIKELSFKTTEKDLMTLVKGAINSGNLKVQTKPEKADVFIICVPTPCIECDGEKKCDLTYLNKAIESIKPYLENGNLIIIESTIPPGTTDDIYKKLSKDKKIYVAHCPERVLPGSILKELVENDRVIGGVDEKSAEMAKEIYETFVTGKIYLTDAKTAEMVKLMENTYRDVNIALANEFAKIAEEIGINVWEAIELANKHPRVNILKPGPGVGGHCISIDPWFIVEKSKNAKLIRTARELNDSMPLFVVEKIKKIIKKDIGKVAIFGVTYKGNVDDTRESPAEKVVSKLIDEGFEVKCYDKYARDFIYPLNSLDEAVEGADIIVILAEHDEYKNFDKEDIKNIASKVKNKIILDTKNILNRELWEKEGFKVYVLGDGKNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57885</accession>
    <name>Y443_METJA</name>
    <protein>
        <recommendedName>
            <fullName>KH domain-containing protein MJ0443</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0443</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64355">
        <property type="entry name" value="C64355"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57885"/>
    <dbReference type="SMR" id="Q57885"/>
    <dbReference type="STRING" id="243232.MJ_0443"/>
    <dbReference type="EnsemblBacteria" id="AAB98430">
        <property type="protein sequence ID" value="AAB98430"/>
        <property type="gene ID" value="MJ_0443"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0443"/>
    <dbReference type="eggNOG" id="arCOG04150">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064992_3_0_2"/>
    <dbReference type="InParanoid" id="Q57885"/>
    <dbReference type="OMA" id="DPLAVWK"/>
    <dbReference type="PhylomeDB" id="Q57885"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039912">
        <property type="entry name" value="PNO1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="PTHR12826:SF13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="SSF54791"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000050165" description="KH domain-containing protein MJ0443">
        <location>
            <begin position="1"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="KH 1" evidence="1">
        <location>
            <begin position="14"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="KH 2" evidence="1">
        <location>
            <begin position="106"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <sequence length="227" mass="25840" checksum="9E59A5FE3CDE8E9C" modified="1996-11-01" version="1">MVIIMVFGNIGQDKSIEILKIPKDRVGVLIGKKGNVKKTIEKELGVKLEIDADGTVTIYGTDKQKDPLAVWKARDIVRAIGRGFNPEIALKLVSDEYVLEVIDIEDYASSDNSIRRLKGRVIGKEGKSRRYIESLTGANVSVYGNTVAIVGEHEPVQIAKEAVEMLLRGASHAKTYKFLERERQKIKRARFELWKKKSDVDELYEKMNPNYEEIEIEEDEDEIEDEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q57887</accession>
    <name>IF1A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Translation initiation factor 1A</fullName>
            <shortName>aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">eIF1A</name>
        <name type="ordered locus">MJ0445</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Protein Sci." volume="10" first="2426" last="2438">
            <title>Structure and dynamics of translation initiation factor aIF-1A from the archaeon Methanococcus jannaschii determined by NMR spectroscopy.</title>
            <authorList>
                <person name="Li W."/>
                <person name="Hoffman D.W."/>
            </authorList>
            <dbReference type="PubMed" id="11714910"/>
            <dbReference type="DOI" id="10.1110/ps.ps.18201"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64355">
        <property type="entry name" value="E64355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869944.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1JT8">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-102"/>
    </dbReference>
    <dbReference type="PDBsum" id="1JT8"/>
    <dbReference type="AlphaFoldDB" id="Q57887"/>
    <dbReference type="SMR" id="Q57887"/>
    <dbReference type="STRING" id="243232.MJ_0445"/>
    <dbReference type="EnsemblBacteria" id="AAB98435">
        <property type="protein sequence ID" value="AAB98435"/>
        <property type="gene ID" value="MJ_0445"/>
    </dbReference>
    <dbReference type="GeneID" id="1451305"/>
    <dbReference type="KEGG" id="mja:MJ_0445"/>
    <dbReference type="eggNOG" id="arCOG01179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109098_1_2_2"/>
    <dbReference type="InParanoid" id="Q57887"/>
    <dbReference type="OMA" id="ADITWRY"/>
    <dbReference type="OrthoDB" id="102432at2157"/>
    <dbReference type="PhylomeDB" id="Q57887"/>
    <dbReference type="EvolutionaryTrace" id="Q57887"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="PTHR21668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145121" description="Translation initiation factor 1A">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="S1-like">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="22"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="32"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="43"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="51"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1JT8"/>
        </source>
    </evidence>
    <sequence length="102" mass="12184" checksum="8082FD686A103FC0" modified="1996-11-01" version="1">MAEQQQEQQIRVRIPRKEENEILGIIEQMLGASRVRVRCLDGKTRLGRIPGRLKNRIWVREGDVVIVKPWEVQGDQKCDIIWRYTKTQVEWLKRKGYLDELL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q57888</accession>
    <name>COFG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase</fullName>
            <ecNumber evidence="3 4 5">4.3.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>FO synthase subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cofG</name>
        <name type="ordered locus">MJ0446</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Arch. Microbiol." volume="180" first="455" last="464">
            <title>Identification of the 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase required for coenzyme F(420) biosynthesis.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="14593448"/>
            <dbReference type="DOI" id="10.1007/s00203-003-0614-8"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2012" name="J. Am. Chem. Soc." volume="134" first="18173" last="18176">
            <title>Biosynthesis of F0, precursor of the F420 cofactor, requires a unique two radical-SAM domain enzyme and tyrosine as substrate.</title>
            <authorList>
                <person name="Decamps L."/>
                <person name="Philmus B."/>
                <person name="Benjdia A."/>
                <person name="White R."/>
                <person name="Begley T.P."/>
                <person name="Berteau O."/>
            </authorList>
            <dbReference type="PubMed" id="23072415"/>
            <dbReference type="DOI" id="10.1021/ja307762b"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2015" name="J. Am. Chem. Soc." volume="137" first="5406" last="5413">
            <title>Biosynthetic versatility and coordinated action of 5'-deoxyadenosyl radicals in deazaflavin biosynthesis.</title>
            <authorList>
                <person name="Philmus B."/>
                <person name="Decamps L."/>
                <person name="Berteau O."/>
                <person name="Begley T.P."/>
            </authorList>
            <dbReference type="PubMed" id="25781338"/>
            <dbReference type="DOI" id="10.1021/ja513287k"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4 5">Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) from 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 5'-deoxyadenosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin + H(+) + L-methionine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:55204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="4.3.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text>Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text>Consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the radical SAM superfamily. CofG family.</text>
    </comment>
    <comment type="sequence caution" evidence="6">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98436" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.3.1.32" evidence="3 4 5"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98436.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64355">
        <property type="entry name" value="F64355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496498.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57888"/>
    <dbReference type="SMR" id="Q57888"/>
    <dbReference type="STRING" id="243232.MJ_0446"/>
    <dbReference type="EnsemblBacteria" id="AAB98436">
        <property type="protein sequence ID" value="AAB98436"/>
        <property type="gene ID" value="MJ_0446"/>
    </dbReference>
    <dbReference type="GeneID" id="1451306"/>
    <dbReference type="KEGG" id="mja:MJ_0446"/>
    <dbReference type="eggNOG" id="arCOG00657">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054174_0_0_2"/>
    <dbReference type="InParanoid" id="Q57888"/>
    <dbReference type="OMA" id="TTACRYT"/>
    <dbReference type="OrthoDB" id="52096at2157"/>
    <dbReference type="PhylomeDB" id="Q57888"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-12178"/>
    <dbReference type="BRENDA" id="4.3.1.32">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016841">
        <property type="term" value="F:ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01611">
        <property type="entry name" value="FO_synth_sub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019939">
        <property type="entry name" value="CofG_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="PTHR43076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00294">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03550">
        <property type="entry name" value="F420_cofG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000147764" description="7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="35"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="14593448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="23072415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="25781338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="358" mass="41582" checksum="F10602568AEB449F" modified="2009-03-03" version="2">MISREEAINFLNSTSSKDILDKLAQINNTFKREYITYSKNVFIPLSKWCRNKCGYCIFREDKPSLMKPNEVKEILLKGDRLGCREALFTFGEHVDENKEIKEQLKSMGYDNILEYLYDLEEWTLNNTSLLPHTNCGILNYDELKMLKDVNASMGLMLENASERLMNTIAHKHSPGKHPKLRIEMIENAGKLKIPFTTGLLIGIGETNEEIVDSLFKIKEIHEKYGHIQEVIIQNFRAKKGIPMENFKEPSPIKMLKVIILAKLILDDISIQIPPNLNRETGQLFLLAGVDDWGGVSPLTRDYVNPEAEWPEIKELREWTEELGLKLKMRLPVYDKYISEEWLSEKVYNKIIEMGWLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q57890</accession>
    <name>Y448_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0448</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0448</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64355">
        <property type="entry name" value="H64355"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57890"/>
    <dbReference type="SMR" id="Q57890"/>
    <dbReference type="STRING" id="243232.MJ_0448"/>
    <dbReference type="EnsemblBacteria" id="AAB98437">
        <property type="protein sequence ID" value="AAB98437"/>
        <property type="gene ID" value="MJ_0448"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0448"/>
    <dbReference type="eggNOG" id="arCOG00503">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036012_0_0_2"/>
    <dbReference type="InParanoid" id="Q57890"/>
    <dbReference type="OMA" id="GHYDHTD"/>
    <dbReference type="PhylomeDB" id="Q57890"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106880" description="Uncharacterized protein MJ0448">
        <location>
            <begin position="1"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="256" mass="29409" checksum="0A4773E57FDECCEE" modified="1996-11-01" version="1">MIKILVDNTAYKKFFAQHGFSALIEINNKRILFDAGQNSITLRENLRLFNEKEGFDYIVLSHGHYDHCDGLKYVIENDLINGKVIAHKDAFLDKYAGNRYIGIDEEIKEYLLKKADLEIIEEPYKIDKDIIVSGYVPREYEYEMEEFQCIKDGKRVKDEVNDDMFLIAKGILITGCSHSGIINVVEYGKKLSEIKGVLGGFHLVGVSDNYLNRIVDYFKSQDFWIMPMHCTGFKALTKLSQLNNFVYGHVGKIIGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57899</accession>
    <name>Y457_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized metallohydrolase MJ0457</fullName>
            <ecNumber>3.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0457</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EC" id="3.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64357">
        <property type="entry name" value="A64357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869956.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57899"/>
    <dbReference type="SMR" id="Q57899"/>
    <dbReference type="STRING" id="243232.MJ_0457"/>
    <dbReference type="EnsemblBacteria" id="AAB98445">
        <property type="protein sequence ID" value="AAB98445"/>
        <property type="gene ID" value="MJ_0457"/>
    </dbReference>
    <dbReference type="GeneID" id="1451318"/>
    <dbReference type="KEGG" id="mja:MJ_0457"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021802_2_2_2"/>
    <dbReference type="InParanoid" id="Q57899"/>
    <dbReference type="OMA" id="STFEPTM"/>
    <dbReference type="OrthoDB" id="14646at2157"/>
    <dbReference type="PhylomeDB" id="Q57899"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000185352" description="Uncharacterized metallohydrolase MJ0457">
        <location>
            <begin position="1"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="154"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="410" mass="47170" checksum="90E7ADB625339D86" modified="1996-11-01" version="1">MDLIEEAIKLESDLIRINSVNPSFGGKGEKEKAEYVKKKLMEYVESYNIENYTLKEYNIIDKYGIERPNIVFKIDFGRDKTLHIISHLDTVPEGDISLWGTNPYEPVIKDGKIYGRGSEDNHKGIVSSLLLLKMIFENNIEPKYNLSLIFVSDEEDGSEYGLKYLLNNFEDEIFKKDDLIIVPDFGTPTGEFVEIGEKGILWIKFNIKGKQCHGSTPENGLNADIVAFNFANELYNGLYEKFDEINSIFLPEYSTFEPTILKNKVENPNTIPGYVEVVFDCRILPTYKIEEVLEFINKFIKNFEFKKYIKHYDNSIKAEITYEILKSENPNYTDENAEIIKELKKAIKNVLNRDAKLCGMGGGTVAAFLRYKGYNVAVWGIGEETAHQPNEHIKIEDLVKMAEVFYEILK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q57945</accession>
    <name>Y525_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0525</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0525</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64365">
        <property type="entry name" value="E64365"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57945"/>
    <dbReference type="STRING" id="243232.MJ_0525"/>
    <dbReference type="EnsemblBacteria" id="AAB98517">
        <property type="protein sequence ID" value="AAB98517"/>
        <property type="gene ID" value="MJ_0525"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0525"/>
    <dbReference type="eggNOG" id="arCOG04830">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3057123_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011317">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09880">
        <property type="entry name" value="DUF2107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036535">
        <property type="entry name" value="EhaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106914" description="Uncharacterized protein MJ0525">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="53" mass="5837" checksum="5394D0250D5A9D98" modified="1996-11-01" version="1">MLNVEVPTIGVSLIFLAYDEALALMTFIAVNAVLSLILIRAVILDAEYKENNQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q57987</accession>
    <name>FEOA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative Fe(2+) transport protein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0567</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Might be involved in Fe(2+) ion uptake.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FeoA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64370">
        <property type="entry name" value="G64370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870071.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57987"/>
    <dbReference type="SMR" id="Q57987"/>
    <dbReference type="STRING" id="243232.MJ_0567"/>
    <dbReference type="EnsemblBacteria" id="AAB98558">
        <property type="protein sequence ID" value="AAB98558"/>
        <property type="gene ID" value="MJ_0567"/>
    </dbReference>
    <dbReference type="GeneID" id="1451432"/>
    <dbReference type="KEGG" id="mja:MJ_0567"/>
    <dbReference type="eggNOG" id="arCOG02102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_150646_6_3_2"/>
    <dbReference type="InParanoid" id="Q57987"/>
    <dbReference type="OMA" id="AMKIMVE"/>
    <dbReference type="OrthoDB" id="114771at2157"/>
    <dbReference type="PhylomeDB" id="Q57987"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055072">
        <property type="term" value="P:iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="SSF50037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0410">Iron transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000106934" description="Putative Fe(2+) transport protein A">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0AEL3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="82" mass="8766" checksum="3F3810EEFC9F81CE" modified="1996-11-01" version="1">MYPLAFAKEGEEVIVKKIDAGCGAMQRLVSMGINIGSKLKVIRNQNGPVIISTKGSNIAIGRGLAMKIMVEDAEYGGENEKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q57990</accession>
    <name>Y570_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0570</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0570</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64371">
        <property type="entry name" value="B64371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870074.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57990"/>
    <dbReference type="SMR" id="Q57990"/>
    <dbReference type="STRING" id="243232.MJ_0570"/>
    <dbReference type="EnsemblBacteria" id="AAB98564">
        <property type="protein sequence ID" value="AAB98564"/>
        <property type="gene ID" value="MJ_0570"/>
    </dbReference>
    <dbReference type="GeneID" id="1451435"/>
    <dbReference type="KEGG" id="mja:MJ_0570"/>
    <dbReference type="eggNOG" id="arCOG00035">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_0_2_2"/>
    <dbReference type="InParanoid" id="Q57990"/>
    <dbReference type="OMA" id="NYALYWA"/>
    <dbReference type="OrthoDB" id="101215at2157"/>
    <dbReference type="PhylomeDB" id="Q57990"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="Alpha_ANH_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005237">
        <property type="entry name" value="MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="PTHR12196"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00289">
        <property type="entry name" value="TIGR00289"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106935" description="Uncharacterized protein MJ0570">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <sequence length="223" mass="25618" checksum="5D1EF288E77B2AF8" modified="1996-11-01" version="1">MKVAVLYSGGKDSNYALYWALKEGFDVKYLVNVESENKESYMFHIPNVHLTELSAEAVGIPLIKLYTKGEKEKEVEDLKKGLEKLDVEGIVTGAVASIYQKSRIDRVCEELGLKSFAPLWHKDPEWILRTVSELFNVRIVGVYAYGLGKEWLGKRITKENIDKLLNICEKYGIHKAFEGGEAETFVFDAPMFKKRIEVVEAEIEWHETWGIYHIKKAKLVDKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58005</accession>
    <name>Y585_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0585</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0585</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64373">
        <property type="entry name" value="A64373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870089.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58005"/>
    <dbReference type="SMR" id="Q58005"/>
    <dbReference type="STRING" id="243232.MJ_0585"/>
    <dbReference type="EnsemblBacteria" id="AAB98583">
        <property type="protein sequence ID" value="AAB98583"/>
        <property type="gene ID" value="MJ_0585"/>
    </dbReference>
    <dbReference type="GeneID" id="1451450"/>
    <dbReference type="KEGG" id="mja:MJ_0585"/>
    <dbReference type="eggNOG" id="ENOG502N58T">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1335056_0_0_2"/>
    <dbReference type="OrthoDB" id="91886at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106945" description="Uncharacterized protein MJ0585">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="205" mass="24327" checksum="9D235C9F16E52A61" modified="1996-11-01" version="1">MKKLAFLILLIVFSNLSLVNAIDDNISNYSKEINELTVKLKELESKNPNDERIEEYKEKLKQLIEKQHELKSQNLKYNETLAYIQSEEYWKMQEEIWEYNNKVMKWFIAISILILGIILATLWILRKDKFLLFLAIFGLIVPFLQFKIPNWLFNILALPLFVYIKFIVPECAEGSFYYSPLITIPISMYGWILIGLAVKFIIKKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58029</accession>
    <name>Y612_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable arogenate/prephenate dehydrogenase</fullName>
            <ecNumber>1.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0612</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the prephenate/arogenate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64376">
        <property type="entry name" value="D64376"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870117.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58029"/>
    <dbReference type="SMR" id="Q58029"/>
    <dbReference type="STRING" id="243232.MJ_0612"/>
    <dbReference type="EnsemblBacteria" id="AAB98605">
        <property type="protein sequence ID" value="AAB98605"/>
        <property type="gene ID" value="MJ_0612"/>
    </dbReference>
    <dbReference type="GeneID" id="1451478"/>
    <dbReference type="KEGG" id="mja:MJ_0612"/>
    <dbReference type="eggNOG" id="arCOG00245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036672_1_0_2"/>
    <dbReference type="InParanoid" id="Q58029"/>
    <dbReference type="OMA" id="TDGLGKW"/>
    <dbReference type="OrthoDB" id="117835at2157"/>
    <dbReference type="PhylomeDB" id="Q58029"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008299">
        <property type="entry name" value="Prep_DH/arog_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006549">
        <property type="entry name" value="PDH_arog_dh_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="SSF54991"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119199" description="Probable arogenate/prephenate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="1">
        <location>
            <begin position="6"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="446" mass="50888" checksum="5968C24E2C9AC2DB" modified="1996-11-01" version="1">MKNTNLTISIIGGTDGLGKWFARYLKNKGFNVIVTGRDIEKGKNVEKELGVEFTNNNIEAAKKGDIVIVAVPINVTERVIKEVAPHVREGCLLMDITSIKEIPSKAMEEHVKEGVTVIPTHPMFGPSTPSLLRQVVILTPSEKHKNTEWFNKVYNFLKKEGAKVIVIPPEKHDRIMGIVQGLTHFAFISLGATLKELNVDIKESRKFASPIYELMISIIGRIIGQNPYLYADIQMFNPRIKEIHETFINQCKEISEIVKNKDREGFVKIMKEAAKHFGSEAKRGAYYSDKAVFALTSEIEKLNKLIGKDVAVKNINSNVVHFGVLKDIEDDYLILNKNGKEQKFNILRVEVFAGDELSKLKKKHLEKKYIDVSVLFKKDVDEEVILNLLKKMFDIEIIDVYEGEKIEEGYKSITFRIYGYNKDELKNIEKEFLKIIKNIGGKERFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58040</accession>
    <name>Y623_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative metallophosphoesterase MJ0623</fullName>
            <ecNumber>3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0623</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64377">
        <property type="entry name" value="G64377"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58040"/>
    <dbReference type="SMR" id="Q58040"/>
    <dbReference type="STRING" id="243232.MJ_0623"/>
    <dbReference type="EnsemblBacteria" id="AAB98618">
        <property type="protein sequence ID" value="AAB98618"/>
        <property type="gene ID" value="MJ_0623"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0623"/>
    <dbReference type="eggNOG" id="arCOG01141">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063749_3_2_2"/>
    <dbReference type="InParanoid" id="Q58040"/>
    <dbReference type="OMA" id="IVVYVYE"/>
    <dbReference type="PhylomeDB" id="Q58040"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110165">
        <property type="term" value="C:cellular anatomical entity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="PTHR11124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155611" description="Putative metallophosphoesterase MJ0623">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="192" mass="21361" checksum="D56A8AC4287CE6D4" modified="1996-11-01" version="1">MRDGEGIWGRGRTGGHAHPKEAMHTTFFILGGTMLIGVISDTHLYDRAFELPKAVFDEFSNVDLIIHCGDVTDKEILDSLKDLAKVVAVKGNMDYLNLPRKEILEINDIKIGVIHGDVVYPRGDRLKLRLLGKEMGVDVLISGHTHTPFIDDCRDILLLNPGSPTVPRCPLKSIMKLSVEDKLEAKLIPIEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58105</accession>
    <name>Y694_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized NOP5 family protein MJ0694</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0694</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
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    <sequence length="414" mass="47799" checksum="A9092EFC3C82C407" modified="1996-11-01" version="1">MIYVTFTPYGAFGVKDNKEVSGLEDIEYKKLFNEEEIPDIMFKLKTQPNKIADELKEEWGDEIKLETLSTEPFNIGEFLRNNLFKVGKELGYFNNYDEFRKKMHYWSTELTKKVIKSYAQQKDKIIIQVAEAISDLDKTLNLLSERLREWYSLYFPELDHLVNKHEVYANLITKLGKRKNFTKSQLKKILPSKLAGKIAEAAKNSMGGELEDYDLDVIVKFAEEINHLYEKRKELYNYLEKLMNEEAPNITKLAGVSLGARLIGLAGGLEKLAKMPASTIQVLGAEKALFAHLRMGVEPPKHGIIYNHPLIQGSPHWQRGKIARALACKLAIAARADYVGDYIADELLEKLNKRVEEIRRKYPKPPKKKKKEKPKAKKKEKKGKKEKSKKKKDKKKDKKGKKERKVIGKTKSRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58125</accession>
    <name>HMDX_METJA</name>
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        <recommendedName>
            <fullName>H(2)-forming methylenetetrahydromethanopterin dehydrogenase-related protein MJ0715</fullName>
        </recommendedName>
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        <name type="ordered locus">MJ0715</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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    <keyword id="KW-0560">Oxidoreductase</keyword>
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    </evidence>
    <sequence length="338" mass="36854" checksum="339B264EA44B013B" modified="1996-11-01" version="1">MKISIYGAGNQRLYLEQLKVPEKFGGEPPYGGAGMAIEFAKAGHDVVLSEPNRDVMSDDLWKKVEDAGVKVVSDDIEAAKHGEIHVLFTPFGRITLNIANTIIEHVPENAIICNTCTIPTPVLYRSLEGILRLKRRDVGISSMHPTGVPGTPSQKYYTIAGKALEGKEYATEDQINKLVELVKSVGKIPYVTPADVVPAVADMGALVTAVALVGVLDYYRVGTQIINAPKDMIEKQILISLQTIASIIETSGMEGLMKVFNKDALLSSAKNMLIDERQEDLNLALKIIEEFDKSTIGEKDISQTYLVAPQALIKEAVSLIGKSAVEGMIRRSSNKLFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58138</accession>
    <name>COOS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Carbon monoxide dehydrogenase</fullName>
            <shortName>CODH</shortName>
            <ecNumber evidence="1">1.2.7.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cooS</name>
        <name type="ordered locus">MJ0728</name>
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    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Biochemistry" volume="41" first="2097" last="2105">
            <title>The Ni-containing carbon monoxide dehydrogenase family: light at the end of the tunnel?</title>
            <authorList>
                <person name="Lindahl P.A."/>
            </authorList>
            <dbReference type="PubMed" id="11841199"/>
            <dbReference type="DOI" id="10.1021/bi015932+"/>
        </citation>
        <scope>REVIEW</scope>
    </reference>
    <comment type="function">
        <text evidence="1">CODH oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (possibly CooH).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>CO + H2O + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + 2 H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 3 [4Fe-4S] clusters per homodimer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="1">Binds 2 [Ni-4Fe-5S] clusters per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster is to be the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">This protein lacks the conserved Cys in position 45; it is replaced by a Tyr. It is therefore possible that the D-cluster is either altered or missing in this protein, which may not form homodimers.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64390">
        <property type="entry name" value="H64390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870233.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58138"/>
    <dbReference type="SMR" id="Q58138"/>
    <dbReference type="STRING" id="243232.MJ_0728"/>
    <dbReference type="PRIDE" id="Q58138"/>
    <dbReference type="EnsemblBacteria" id="AAB98724">
        <property type="protein sequence ID" value="AAB98724"/>
        <property type="gene ID" value="MJ_0728"/>
    </dbReference>
    <dbReference type="GeneID" id="1451605"/>
    <dbReference type="KEGG" id="mja:MJ_0728"/>
    <dbReference type="eggNOG" id="arCOG02429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030631_0_0_2"/>
    <dbReference type="InParanoid" id="Q58138"/>
    <dbReference type="OMA" id="PEYMEQK"/>
    <dbReference type="OrthoDB" id="5135at2157"/>
    <dbReference type="PhylomeDB" id="Q58138"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:carbon-monoxide dehydrogenase (ferredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006091">
        <property type="term" value="P:generation of precursor metabolites and energy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01915">
        <property type="entry name" value="CODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016101">
        <property type="entry name" value="CO_DH_a-bundle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010047">
        <property type="entry name" value="CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005023">
        <property type="entry name" value="CODH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01702">
        <property type="entry name" value="CO_DH_cata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000157143" description="Carbon monoxide dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="336"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="444"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="516"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-5S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:177874"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9F8A8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="624" mass="67408" checksum="FFB6AA3376D1050E" modified="1998-06-01" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-05-27" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58148</accession>
    <name>Y738_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0738</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0738</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64392">
        <property type="entry name" value="B64392"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58148"/>
    <dbReference type="SMR" id="Q58148"/>
    <dbReference type="STRING" id="243232.MJ_0738"/>
    <dbReference type="EnsemblBacteria" id="AAB98733">
        <property type="protein sequence ID" value="AAB98733"/>
        <property type="gene ID" value="MJ_0738"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0738"/>
    <dbReference type="eggNOG" id="arCOG02797">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366171_0_0_2"/>
    <dbReference type="OMA" id="TNHPLYE"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036825">
        <property type="entry name" value="MTH865-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024093">
        <property type="entry name" value="Uncharacterised_MTH865"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07747">
        <property type="entry name" value="MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69025">
        <property type="entry name" value="SSF69025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107009" description="Uncharacterized protein MJ0738">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <sequence length="90" mass="10276" checksum="121DAA6854FBF3ED" modified="1996-11-01" version="1">MMITTNHPLYEALRDIQEFKLRLVEYFKDKDVFPIKNKVELAEALPCGISLPCGEIEAAELVKLLTDNDFPIKDPEDLAMKLANKCPIKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58160</accession>
    <name>Y750_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0750</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0750</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64393">
        <property type="entry name" value="F64393"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870255.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58160"/>
    <dbReference type="STRING" id="243232.MJ_0750"/>
    <dbReference type="EnsemblBacteria" id="AAB98746">
        <property type="protein sequence ID" value="AAB98746"/>
        <property type="gene ID" value="MJ_0750"/>
    </dbReference>
    <dbReference type="GeneID" id="1451627"/>
    <dbReference type="KEGG" id="mja:MJ_0750"/>
    <dbReference type="eggNOG" id="arCOG02772">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033147_1_0_2"/>
    <dbReference type="InParanoid" id="Q58160"/>
    <dbReference type="OMA" id="CPVGAFY"/>
    <dbReference type="OrthoDB" id="65742at2157"/>
    <dbReference type="PhylomeDB" id="Q58160"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12801">
        <property type="entry name" value="Fer4_5"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159312" description="Uncharacterized protein MJ0750">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="3">
        <location>
            <begin position="178"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="3">
        <location>
            <begin position="204"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="198"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="238" mass="27777" checksum="6B4F7FB996E941EF" modified="1996-11-01" version="1">MDKIQILRKISQTLFFIYFVFLTSFCLCFFGIIEKFILKGSVGQLIAKLVVIVVLTLILGRVFCGWMCPLGFLFELMYKLRMKLFMKKKLPTVNEEVHNKLIYLRYVVLILSLVLTYYLSIYAFCQVCPIGFLTNLYGTVISLIILIFFLSLSFFVPMAFCRYFCPLGAFLSIFSIKPFFQLKTNNNCVKCKLCEFKCPMQIKITEKLDQKECIRCFECKSSCKKDALSFSYAFKKRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58181</accession>
    <name>CBIL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable cobalt-precorrin-2 C(20)-methyltransferase</fullName>
            <ecNumber>2.1.1.151</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cbiL</name>
        <name type="ordered locus">MJ0771</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Co-precorrin-2 + S-adenosyl-L-methionine = Co-precorrin-3 + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:17997"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60053"/>
            <dbReference type="ChEBI" id="CHEBI:60060"/>
            <dbReference type="EC" id="2.1.1.151"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 2/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.151"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64396">
        <property type="entry name" value="C64396"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58181"/>
    <dbReference type="SMR" id="Q58181"/>
    <dbReference type="STRING" id="243232.MJ_0771"/>
    <dbReference type="EnsemblBacteria" id="AAB98764">
        <property type="protein sequence ID" value="AAB98764"/>
        <property type="gene ID" value="MJ_0771"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0771"/>
    <dbReference type="eggNOG" id="arCOG00648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076014_2_1_2"/>
    <dbReference type="InParanoid" id="Q58181"/>
    <dbReference type="OMA" id="MEYPVTT"/>
    <dbReference type="PhylomeDB" id="Q58181"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00224"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043781">
        <property type="term" value="F:cobalt-factor II C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150411" description="Probable cobalt-precorrin-2 C(20)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="230" mass="26267" checksum="4FFD926E9B441FFB" modified="1996-11-01" version="1">MNKLVKKVYGVGVGVGDKKLLTLKALEVLKKVDKIFVPVSKKGKKSIAYEIIKDYVDGKNIEELLFPMIKDKERLKKYWENALEKVLKEDGEVAIITIGDPTLYSTFSYVWKLLKERGVEVEIVNGISSIFASAAALNIPLVEGDEKLCILPQGKDLEKYIDEFDTIIIMKTKNLNEKLSVIKNRDDYIIGLVKRATFEDEKVVIGKLDEINFDEFNDYLSLAIIKRFKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58195</accession>
    <name>Y785_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0785</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0785</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64398">
        <property type="entry name" value="A64398"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58195"/>
    <dbReference type="SMR" id="Q58195"/>
    <dbReference type="STRING" id="243232.MJ_0785"/>
    <dbReference type="EnsemblBacteria" id="AAB98782">
        <property type="protein sequence ID" value="AAB98782"/>
        <property type="gene ID" value="MJ_0785"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0785"/>
    <dbReference type="eggNOG" id="arCOG00658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033172_0_0_2"/>
    <dbReference type="InParanoid" id="Q58195"/>
    <dbReference type="OMA" id="IMGFNPY"/>
    <dbReference type="PhylomeDB" id="Q58195"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023858">
        <property type="entry name" value="RSAM_HmdB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="PTHR22976"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00330">
        <property type="entry name" value="HMD_cofactor_maturase_(HmdB-li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03957">
        <property type="entry name" value="rSAM_HmdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000185568" description="Uncharacterized protein MJ0785">
        <location>
            <begin position="1"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="78"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="375" mass="42810" checksum="1467DF2B76A89999" modified="1996-11-01" version="1">MKYTYKISKVKISGDLMVFGKIEEEFKEFLESRRKYNEFIKNGLIDEDEALKLFKIDNWRDYLKLFDIASRVRDYFKKKIEITSTIHITNICHVNPKCLYCGFAAGTSKEGYYEPFRLTDEEIKKSAIAIEESGIKRVSCSSAHGYQGKEVIRALKIVKKYTNLEVLVNAGADLTEESIKELKKYGIDTICCNLETINENLFKKVKPGEELEDRIRVCKLVNKYDIELSTGLLIGIGESYEDRVEHLFYLKNELNVGEIPIMGFNPYKGTPMENHPKCSALEQAKTIAITRLIFPNIRITSPTPTIGAELVQFALFGGASNVATVIPKNHPMNVKGVGNPKTGNLEEVVKMIMDLGLKPKLDWRRYENYLKIYGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58200</accession>
    <name>Y790_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0790</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0790</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64398">
        <property type="entry name" value="F64398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870297.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58200"/>
    <dbReference type="STRING" id="243232.MJ_0790"/>
    <dbReference type="EnsemblBacteria" id="AAB98784">
        <property type="protein sequence ID" value="AAB98784"/>
        <property type="gene ID" value="MJ_0790"/>
    </dbReference>
    <dbReference type="GeneID" id="1451669"/>
    <dbReference type="KEGG" id="mja:MJ_0790"/>
    <dbReference type="eggNOG" id="arCOG04880">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1140581_0_0_2"/>
    <dbReference type="OMA" id="WKGHTIL"/>
    <dbReference type="OrthoDB" id="61635at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107041" description="Uncharacterized protein MJ0790">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="229" mass="25893" checksum="7636AC4674E85379" modified="1996-11-01" version="1">MNWKGHTILGIIFGLPFISSPEQIFLALAGALYPDLDHDVKEDIVKRGLLISGGIVFINILLYFFDKHLFNVDLFILGVLILLIYLIPYFSDHRGLTHTFWSLLFVSSILGYLAYKLSFISSVFAGLISLLMVTNEVLLGRVMIFAVFAWAVLDILNPNINVNGALYYILPVVFGYLSHLVGDTMTPAGVRAFYPLSNYKLRKKEGYILVAIWALMAVYVWKYMILSLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58231</accession>
    <name>Y821_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative uncharacterized protein MJ0821</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0821</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Could be the product of a pseudogene. MJ0821, MJ0820 and MJ0819 respectively represent the N-terminal, central and C-terminal sections of other members of this family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64402">
        <property type="entry name" value="E64402"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58231"/>
    <dbReference type="STRING" id="243232.MJ_0821"/>
    <dbReference type="EnsemblBacteria" id="AAB98833">
        <property type="protein sequence ID" value="AAB98833"/>
        <property type="gene ID" value="MJ_0821"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0821"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3003150_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="uncertain"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184683" description="Putative uncharacterized protein MJ0821">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="56" mass="6512" checksum="7E19C78530493060" modified="1996-11-01" version="1">MKFFNREKEINKILSIIEGEPNLIYFIYGSLNSGKFNLTEETQFIYSIVVTDGLFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58237</accession>
    <name>Y827_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0827</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0827</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64403">
        <property type="entry name" value="C64403"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58237"/>
    <dbReference type="SMR" id="Q58237"/>
    <dbReference type="STRING" id="243232.MJ_0827"/>
    <dbReference type="EnsemblBacteria" id="AAB98826">
        <property type="protein sequence ID" value="AAB98826"/>
        <property type="gene ID" value="MJ_0827"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0827"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024949_3_3_2"/>
    <dbReference type="InParanoid" id="Q58237"/>
    <dbReference type="OMA" id="MFQVTDP"/>
    <dbReference type="PhylomeDB" id="Q58237"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_HflKC_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="PTHR10264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="SSF117892"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000094064" description="Uncharacterized protein MJ0827">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="199" mass="22809" checksum="2545CE07DD54337E" modified="1996-11-01" version="1">MKVNDMFWFWLILGIIALFIIVKAIVIVNQYEGGLIFRLGRVIGKLKPGINIIIPFLDVPVKVDMRTRVTDIPPQEMITKDNAVVKVDAVVYYRVIDVEKAILEVEDYEYAIINLAQTTLRAIIGSMELDEVLNKREYINSKLLEILDRETDAWGVRIEKVEVKEIDPPEDIKNAMAQQMKAERLKRAAILEAEGEKPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58239</accession>
    <name>RF1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">prf1</name>
        <name type="ordered locus">MJ0829</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="FEBS Lett." volume="472" first="213" last="216">
            <title>Translation termination factor aRF1 from the archaeon Methanococcus jannaschii is active with eukaryotic ribosomes.</title>
            <authorList>
                <person name="Dontsova M."/>
                <person name="Frolova L."/>
                <person name="Vassilieva J."/>
                <person name="Piendl W."/>
                <person name="Kisselev L."/>
                <person name="Garber M."/>
            </authorList>
            <dbReference type="PubMed" id="10788613"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(00)01466-6"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98828" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98828.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64403">
        <property type="entry name" value="E64403"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58239"/>
    <dbReference type="SMR" id="Q58239"/>
    <dbReference type="STRING" id="243232.MJ_0829"/>
    <dbReference type="EnsemblBacteria" id="AAB98828">
        <property type="protein sequence ID" value="AAB98828"/>
        <property type="gene ID" value="MJ_0829"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0829"/>
    <dbReference type="eggNOG" id="arCOG01742">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_3_0_2"/>
    <dbReference type="InParanoid" id="Q58239"/>
    <dbReference type="OMA" id="GQEMEVV"/>
    <dbReference type="PhylomeDB" id="Q58239"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00424">
        <property type="entry name" value="Rel_fact_arch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_1_Pelota"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020918">
        <property type="entry name" value="Peptide_chain-rel_aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PTHR10113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="SSF55481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03676">
        <property type="entry name" value="aRF1/eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000143174" description="Peptide chain release factor subunit 1">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10788613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="421" mass="47508" checksum="6F3C642D466A8D5B" modified="2001-08-14" version="2">MASTDSKQLYLFKKMLKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQASNIKSKSTRKNVQSAIEAILQRLKLLKEPLEKGVVIFAGMVPRSGPGTEKMETYVIEPPEPIKTYIYRCDSEFYLEPLEEFLEDKDAYGVILVDRNEATIALVKGRNINILKKLTSGVPGKFKAGGQSARRLERLIDLAAHEFLQRVGQKANEQFLPLLQEKKLRGILVGGPGHTKNEFVEGDYLHHELKKIVLDTFDLCYTEEFGIRELLEKAAPLLKDVELMKEREAVQRFLKELIKEDGGLACYGEKEVLEALMMGAVDTLIVSEELEKYKVKIACNNCDYLEEKTVNKLELIKLEEELKNAQCPKCGGALSIVEEKDYIEYLSELCEQSGAKLVTVSSETEEGAMILNAFKGIAAILRYKIHQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58251</accession>
    <name>MCRAM_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">[Methyl-coenzyme M reductase subunit alpha]-arginine C-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0841</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Radical SAM methyltransferase that is responsible for the C(5)-methylation of 'Arg-274' of the methyl-coenzyme M reductase (MCR) subunit alpha McrA. This post-translational methylation, despite being not essential in vivo, plays a role for the stability and structural integrity of MCR.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds at least 1 [4Fe-4S] cluster. This cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the radical SAM superfamily. MCR alpha subunit C-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64405">
        <property type="entry name" value="A64405"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870355.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58251"/>
    <dbReference type="STRING" id="243232.MJ_0841"/>
    <dbReference type="DNASU" id="1451729"/>
    <dbReference type="EnsemblBacteria" id="AAB98846">
        <property type="protein sequence ID" value="AAB98846"/>
        <property type="gene ID" value="MJ_0841"/>
    </dbReference>
    <dbReference type="GeneID" id="1451729"/>
    <dbReference type="KEGG" id="mja:MJ_0841"/>
    <dbReference type="eggNOG" id="arCOG00950">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_668380_0_0_2"/>
    <dbReference type="InParanoid" id="Q58251"/>
    <dbReference type="OMA" id="IHLGYTS"/>
    <dbReference type="OrthoDB" id="21265at2157"/>
    <dbReference type="PhylomeDB" id="Q58251"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017672">
        <property type="entry name" value="MA_4551-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03278">
        <property type="entry name" value="methan_mark_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107078" description="[Methyl-coenzyme M reductase subunit alpha]-arginine C-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="2"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8THG6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="415" mass="46721" checksum="0F67E63793A587F1" modified="1996-11-01" version="1">MKEANLLIDLRGEPGINCNGFCKFCYFRKVNKNNPQPFGCRYCQFTVGCDYCMYSVREINGDFIPLPFALMELQSSLLFKRYSKVNLTAGGDVSCYPQLEELCKAINNIGLKIHLGYTSGKGFDNVEIAKNLVDYGVDEVTFSVFSTNPKLRKEWMNDKNAETALKCLRYFCENCEVHCAIIVIPGVNDGEELKKTVSDLVDWGANAVILMRFANSEEQGLILGNAPLIEGIKPHSVEEFKNIVDEIHNEFGDYIRVTGTPLHDPVAGTPFALAKEENSHILERLKDKINGEATIITGNVAYPFLKKIFDETSVNVVKVNKDIADLITAKDLEKLDLKDVKETVFIPPKAFVHDRVAEEILRRDGVDRIVVRGVEQLTLDGEVSGIYTREEALKFEIEAFEELIGMINFFGMKKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58274</accession>
    <name>HDRC2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CoB--CoM heterodisulfide reductase iron-sulfur subunit C 2</fullName>
            <ecNumber evidence="3">1.8.98.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hdrC2</name>
        <name type="ordered locus">MJ0864</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 2 [4Fe-4S] clusters per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The heterodisulfide reductase is composed of three subunits; HdrA, HdrB and HdrC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HdrC family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.-" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64407">
        <property type="entry name" value="H64407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870379.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58274"/>
    <dbReference type="SMR" id="Q58274"/>
    <dbReference type="STRING" id="243232.MJ_0864"/>
    <dbReference type="PRIDE" id="Q58274"/>
    <dbReference type="EnsemblBacteria" id="AAB98869">
        <property type="protein sequence ID" value="AAB98869"/>
        <property type="gene ID" value="MJ_0864"/>
    </dbReference>
    <dbReference type="GeneID" id="1451753"/>
    <dbReference type="KEGG" id="mja:MJ_0864"/>
    <dbReference type="eggNOG" id="arCOG00964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121273_0_0_2"/>
    <dbReference type="InParanoid" id="Q58274"/>
    <dbReference type="OMA" id="AQKGNMA"/>
    <dbReference type="OrthoDB" id="102216at2157"/>
    <dbReference type="PhylomeDB" id="Q58274"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017680">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03290">
        <property type="entry name" value="CoB_CoM_SS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000150074" description="CoB--CoM heterodisulfide reductase iron-sulfur subunit C 2">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="67"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q6LY39"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="186" mass="21049" checksum="DCF74D7A6996117A" modified="1996-11-01" version="1">MAVIKLDEVNKNFVNEVIEAGKLVLGEDIVKSIKACYQCGTCTGSCPSGRRTAYRTRKVLRKVLLGLDDVLDSDDIWYCTTCYTCYERCPRDVKITEIIKTLRNIAAQKGNMALAHRKTASYVLRFGHAVPANNQIVELRGKLGLPAKSPTAQFSEKDLEEVRTLIKELKFDKLIAFDWEKMDLKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q58323</accession>
    <name>COABC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2 5">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="2">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="2 4">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="2 4">PPC decarboxylase</shortName>
                <shortName evidence="2 5">PPC-DC</shortName>
                <ecNumber evidence="2 3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2 4">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="2 5">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="2 3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="2 4">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="2 4">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="2 4">PPC synthetase</shortName>
                <shortName evidence="2 5">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="2" type="primary">coaBC</name>
        <name evidence="4" type="synonym">dfp</name>
        <name type="ordered locus">MJ0913</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="5435" last="5444">
            <title>4'-phosphopantetheine biosynthesis in Archaea.</title>
            <authorList>
                <person name="Kupke T."/>
                <person name="Schwarz W."/>
            </authorList>
            <dbReference type="PubMed" id="16371361"/>
            <dbReference type="DOI" id="10.1074/jbc.m510056200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF HIS-87; HIS-139; ASN-141; GLU-167; GLU-168; ASN-217 AND LYS-310</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>(R)-4'-phosphopantothenate + CTP + L-cysteine = CMP + diphosphate + H(+) + N-[(R)-4-phosphopantothenoyl]-L-cysteine</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1 2">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homododecamer. The CoaC domain is responsible for dodecamer formation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="2 3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="2 3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64414">
        <property type="entry name" value="A64414"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58323"/>
    <dbReference type="SMR" id="Q58323"/>
    <dbReference type="STRING" id="243232.MJ_0913"/>
    <dbReference type="EnsemblBacteria" id="AAB98918">
        <property type="protein sequence ID" value="AAB98918"/>
        <property type="gene ID" value="MJ_0913"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0913"/>
    <dbReference type="eggNOG" id="arCOG01704">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033319_0_3_2"/>
    <dbReference type="InParanoid" id="Q58323"/>
    <dbReference type="OMA" id="LDMIVAN"/>
    <dbReference type="PhylomeDB" id="Q58323"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-21896"/>
    <dbReference type="BRENDA" id="6.3.2.5">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="SSF102645"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="SSF52507"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0288">FMN</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000232696" description="Coenzyme A biosynthesis bifunctional protein CoaBC">
        <location>
            <begin position="1"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="2">
        <location>
            <begin position="198"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Loss of PPC decarboxylase activity." evidence="3">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in PPC decarboxylase activity." evidence="3">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No change in PPC decarboxylase activity." evidence="3">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in PPC decarboxylase activity." evidence="3">
        <original>H</original>
        <variation>D</variation>
        <variation>N</variation>
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No change in PPC decarboxylase activity." evidence="3">
        <original>N</original>
        <variation>D</variation>
        <location>
            <position position="141"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No change in PPC decarboxylase activity." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in PPC decarboxylase activity." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <variation>D</variation>
        <variation>K</variation>
        <location>
            <position position="168"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Shows only residual PPC synthetase activity." evidence="3">
        <original>N</original>
        <variation>H</variation>
        <location>
            <position position="217"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Shows very low PPC synthetase activity." evidence="3">
        <original>K</original>
        <variation>Q</variation>
        <location>
            <position position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0ABQ0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16371361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="16371361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="403" mass="45671" checksum="B8B51AA81A115B94" modified="1996-11-01" version="1">MISEIMHPTKLLKGTKSKLLENKKILVAVTSSIAAIETPKLMRELIRHGAEVYCIITEETKKIIGKEALKFGCGNEVYEEITGDIEHILLYNECDCLLIYPATANIISKINLGIADNIVNTTALMFFGNKPIFIVPAMHENMFNAIKRHIDKLKEKDKIYIISPKFEEGKAKVANIEDVVKAVIEKIGNNLKKEGNRVLILNGGTVEFIDKVRVISNLSSGKMGVALAEAFCKEGFYVEVITAMGLEPPYYIKNHKVLTAKEMLNKAIELAKDFDIIISSAAISDFTVESFEGKLSSEEELILKLKRNPKVLEELRRIYKDKVIIGFKAEYNLDEKELINRAKERLNKYNLNMIIANDLSKHYFGDDYIEVYIITKYEVEKISGSKKEISERIVEKVKKLVKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58332</accession>
    <name>Y922_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0922</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0922</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64415">
        <property type="entry name" value="B64415"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870436.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2P9M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.59 A"/>
        <property type="chains" value="A/B/C/D=1-138"/>
    </dbReference>
    <dbReference type="PDBsum" id="2P9M"/>
    <dbReference type="AlphaFoldDB" id="Q58332"/>
    <dbReference type="SMR" id="Q58332"/>
    <dbReference type="STRING" id="243232.MJ_0922"/>
    <dbReference type="EnsemblBacteria" id="AAB98926">
        <property type="protein sequence ID" value="AAB98926"/>
        <property type="gene ID" value="MJ_0922"/>
    </dbReference>
    <dbReference type="GeneID" id="1451811"/>
    <dbReference type="KEGG" id="mja:MJ_0922"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_9_2_2"/>
    <dbReference type="InParanoid" id="Q58332"/>
    <dbReference type="OMA" id="EDVVFAF"/>
    <dbReference type="OrthoDB" id="104239at2157"/>
    <dbReference type="PhylomeDB" id="Q58332"/>
    <dbReference type="EvolutionaryTrace" id="Q58332"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000107107" description="Uncharacterized protein MJ0922">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="14"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="79"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="41"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="50"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="65"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="75"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="111"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="119"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2P9M"/>
        </source>
    </evidence>
    <sequence length="138" mass="15469" checksum="CCBFFA3534EB48D0" modified="1996-11-01" version="1">MIDTLKNIKVKDVMTKNVITAKRHEGVVEAFEKMLKYKISSLPVIDDENKVIGIVTTTDIGYNLIRDKYTLETTIGDVMTKDVITIHEDASILEAIKKMDISGKKEEIINQLPVVDKNNKLVGIISDGDIIRTISKII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58350</accession>
    <name>Y940_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TPR repeat-containing protein MJ0940</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0940</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64417">
        <property type="entry name" value="D64417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870454.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58350"/>
    <dbReference type="SMR" id="Q58350"/>
    <dbReference type="STRING" id="243232.MJ_0940"/>
    <dbReference type="EnsemblBacteria" id="AAB98944">
        <property type="protein sequence ID" value="AAB98944"/>
        <property type="gene ID" value="MJ_0940"/>
    </dbReference>
    <dbReference type="GeneID" id="1451836"/>
    <dbReference type="KEGG" id="mja:MJ_0940"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003728_2_5_2"/>
    <dbReference type="InParanoid" id="Q58350"/>
    <dbReference type="OMA" id="FETMGYL"/>
    <dbReference type="OrthoDB" id="39628at2157"/>
    <dbReference type="PhylomeDB" id="Q58350"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0802">TPR repeat</keyword>
    <feature type="chain" id="PRO_0000106458" description="TPR repeat-containing protein MJ0940">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 1">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 2">
        <location>
            <begin position="84"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 3">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 4">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 5">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 6">
        <location>
            <begin position="221"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 7">
        <location>
            <begin position="255"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 8">
        <location>
            <begin position="289"/>
            <end position="318"/>
        </location>
    </feature>
    <sequence length="318" mass="36861" checksum="3CA9BCBFF71B4047" modified="1996-11-01" version="1">MEIKNKKLKETSDVLLSLTYLIKSYEYRDRGNLLESLYYLDKALELNPDFKFAKFLKAISLAILGDINKSIECLEDITSNSNDPVAYALLGQLYELLGNFDNALECYEKSLGIEEKFATAFFLKVLCLGLSGKYDELLKCCDRLISFAPNFIPAYIIKANMLRKLGRYEEALACVNKVLELKENDTNAIYLKALILNRIGNCDEALKYYEKLIDELNVTWIEVIREAIYLSFLFNKLDKAEKYIEMGLKLRPDDASLWYFKGKLYEKQNKFEEALKYYNKAIQLMPHHTKALLAKARVLEKLGRIEESIECYNKALDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-01-11" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58356</accession>
    <name>TRM1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trm1</name>
        <name type="ordered locus">MJ0946</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) + N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64418">
        <property type="entry name" value="B64418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870460.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58356"/>
    <dbReference type="SMR" id="Q58356"/>
    <dbReference type="STRING" id="243232.MJ_0946"/>
    <dbReference type="EnsemblBacteria" id="AAB98948">
        <property type="protein sequence ID" value="AAB98948"/>
        <property type="gene ID" value="MJ_0946"/>
    </dbReference>
    <dbReference type="GeneID" id="1451843"/>
    <dbReference type="KEGG" id="mja:MJ_0946"/>
    <dbReference type="eggNOG" id="arCOG01219">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_5_1_2"/>
    <dbReference type="InParanoid" id="Q58356"/>
    <dbReference type="OMA" id="VFYNPVM"/>
    <dbReference type="OrthoDB" id="70865at2157"/>
    <dbReference type="PhylomeDB" id="Q58356"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="PTHR10631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000147683" description="tRNA (guanine(26)-N(2))-dimethyltransferase">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="domain" description="Trm1 methyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <sequence length="374" mass="42588" checksum="E6394A090120165C" modified="1996-11-01" version="1">MILKEGEVVFEVPDKLTVTKKDEVFYNPRMKTCRDISIAVIQAFLNLYHKRDKFYIADALAGSGIRGLRYAKELEFNGELKVFLNDINPKAYEKIINNAKLNEIENIDVFNEDANTFLSKHFRFFNVVDIDPFGSPAPYVEQAIRALVTRNGLLCLTATDTAALCGRSKKSCLRKYLAYPLFGRDCHEFALRVLVGYVMRMATKYELALKPVFCHATDHYVRVYLVTDRGAKRADKVFEMLGYVKDVNGIKIIKKFEEGYEKGFAGPLYIGNLYDKALVEEALKIAEKREFSERVLKILNAIKGESAINQVGCYDTHQIGKMLKISVPPMQDIINKLKEMGFNAVVTHYNPKGIKTDATLKNVIEAIYQCTKIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q58357</accession>
    <name>SYI_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Isoleucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Isoleucyl-tRNA synthetase</fullName>
            <shortName evidence="1">IleRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ileS</name>
        <name type="ordered locus">MJ0947</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64418">
        <property type="entry name" value="C64418"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870461.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58357"/>
    <dbReference type="SMR" id="Q58357"/>
    <dbReference type="STRING" id="243232.MJ_0947"/>
    <dbReference type="PRIDE" id="Q58357"/>
    <dbReference type="EnsemblBacteria" id="AAB98949">
        <property type="protein sequence ID" value="AAB98949"/>
        <property type="gene ID" value="MJ_0947"/>
    </dbReference>
    <dbReference type="GeneID" id="1451844"/>
    <dbReference type="KEGG" id="mja:MJ_0947"/>
    <dbReference type="eggNOG" id="arCOG00807">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_1_2"/>
    <dbReference type="InParanoid" id="Q58357"/>
    <dbReference type="OMA" id="HLGTAWN"/>
    <dbReference type="OrthoDB" id="4914at2157"/>
    <dbReference type="PhylomeDB" id="Q58357"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="PTHR42780"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000098580" description="Isoleucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="600"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="603"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02003"/>
        </source>
    </evidence>
    <sequence length="1039" mass="122234" checksum="7163C46B4AE08AC0" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58366</accession>
    <name>Y956_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0956</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0956</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64419">
        <property type="entry name" value="D64419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870470.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58366"/>
    <dbReference type="STRING" id="243232.MJ_0956"/>
    <dbReference type="EnsemblBacteria" id="AAB98969">
        <property type="protein sequence ID" value="AAB98969"/>
        <property type="gene ID" value="MJ_0956"/>
    </dbReference>
    <dbReference type="GeneID" id="1451854"/>
    <dbReference type="KEGG" id="mja:MJ_0956"/>
    <dbReference type="eggNOG" id="arCOG00043">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1067976_0_0_2"/>
    <dbReference type="OMA" id="KWIMKAN"/>
    <dbReference type="OrthoDB" id="58603at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107120" description="Uncharacterized protein MJ0956">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <sequence length="260" mass="30867" checksum="80F2F8D67C8F0D49" modified="1996-11-01" version="1">MHEDVWSLIKETNHKTILYSHGMKNRVLLGYLLKLRGKYNFDFEAVTFISDRSPKWVREEIKWIMKANDVKHRFIECGKCKISLENNGHDYHGSCVVWEGIKNFEGGVVASSIGEHIKDKERFLTWCNDYNLFPPFIHLNVSRERLVKEFESLDKNLISSCYHACVFCPILYKLGKSLIKSEIFYKKKILTDNERKEHALNEFYDAMKEQDIGRMINSVKIYYNKYIRELDEPYADYIKSRDVEMFDKLVNKCKEYLGVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q58378</accession>
    <name>PATS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Soluble P-type ATPase-like phosphatase</fullName>
            <ecNumber>3.6.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">patS</name>
        <name type="ordered locus">MJ0968</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="FEBS Lett." volume="471" first="99" last="102">
            <title>Soluble P-type ATPase from an archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Ogawa H."/>
                <person name="Haga T."/>
                <person name="Toyoshima C."/>
            </authorList>
            <dbReference type="PubMed" id="10760521"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(00)01374-0"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="FEBS Lett." volume="543" first="31" last="36">
            <title>The Methanocaldococcus jannaschii protein Mj0968 is not a P-type ATPase.</title>
            <authorList>
                <person name="Bramkamp M."/>
                <person name="Gassel M."/>
                <person name="Herkenhoff-Hesselmann B."/>
                <person name="Bertrand J."/>
                <person name="Altendorf K."/>
            </authorList>
            <dbReference type="PubMed" id="12753900"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(03)00372-7"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="function">
        <text>Most probably acts as a phosphatase in the cytosol.</text>
    </comment>
    <comment type="cofactor">
        <cofactor>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text>Inhibited by orthovanadate.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text>Optimum temperature is 50 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64420">
        <property type="entry name" value="H64420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870482.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58378"/>
    <dbReference type="SMR" id="Q58378"/>
    <dbReference type="STRING" id="243232.MJ_0968"/>
    <dbReference type="EnsemblBacteria" id="AAB98973">
        <property type="protein sequence ID" value="AAB98973"/>
        <property type="gene ID" value="MJ_0968"/>
    </dbReference>
    <dbReference type="GeneID" id="1451866"/>
    <dbReference type="KEGG" id="mja:MJ_0968"/>
    <dbReference type="eggNOG" id="arCOG01579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052165_0_0_2"/>
    <dbReference type="InParanoid" id="Q58378"/>
    <dbReference type="OMA" id="GISYCNP"/>
    <dbReference type="OrthoDB" id="66892at2157"/>
    <dbReference type="PhylomeDB" id="Q58378"/>
    <dbReference type="SABIO-RK" id="Q58378"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000046397" description="Soluble P-type ATPase-like phosphatase">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="1">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="273" mass="30739" checksum="FC6D56FD985E4381" modified="1998-11-01" version="3">MKVAIVFDSAGTLVKIMRVIKDLKKNKFICNSQTVDIVDKKKGRALVIIKEDPLKVVDKENPEKLISDLLKEVEIGISYCNPPINREGIFKDRKTKVKELQEPLNILKRYEVETGYGSALIIDTYAGEVEYTIATAGCLFKEVKETIKQLKDLGVKVFIASGDRKGFIKRLAEITGVDERYIMAEAHQELKRDLIRNLKKEGYFTIMVGDGANDVPAMIESDLAVVTLQNGNVSRRALETADIKIYNIKEIVDICKKVINGEIKGRMQIKECS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58408</accession>
    <name>DACZ_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diadenylate cyclase</fullName>
            <shortName evidence="1">DAC</shortName>
            <ecNumber evidence="1">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="1">c-di-AMP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Diadenylyl cyclase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">dacZ</name>
        <name evidence="2" type="synonym">dacY</name>
        <name type="ordered locus">MJ1002</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2013" name="Nat. Rev. Microbiol." volume="11" first="513" last="524">
            <title>Cyclic di-AMP: another second messenger enters the fray.</title>
            <authorList>
                <person name="Corrigan R.M."/>
                <person name="Gruendling A."/>
            </authorList>
            <dbReference type="PubMed" id="23812326"/>
            <dbReference type="DOI" id="10.1038/nrmicro3069"/>
        </citation>
        <scope>GENE NAME</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2015" name="J. Am. Chem. Soc." volume="137" first="6432" last="6435">
            <title>RNA-based fluorescent biosensors for live cell imaging of second messenger cyclic di-AMP.</title>
            <authorList>
                <person name="Kellenberger C.A."/>
                <person name="Chen C."/>
                <person name="Whiteley A.T."/>
                <person name="Portnoy D.A."/>
                <person name="Hammond M.C."/>
            </authorList>
            <dbReference type="PubMed" id="25965978"/>
            <dbReference type="DOI" id="10.1021/jacs.5b00275"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>EXPRESSION IN E.COLI</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP) (PubMed:25965978). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate cyclase family. DacZ subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64425">
        <property type="entry name" value="A64425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870515.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58408"/>
    <dbReference type="SMR" id="Q58408"/>
    <dbReference type="STRING" id="243232.MJ_1002"/>
    <dbReference type="EnsemblBacteria" id="AAB99005">
        <property type="protein sequence ID" value="AAB99005"/>
        <property type="gene ID" value="MJ_1002"/>
    </dbReference>
    <dbReference type="GeneID" id="1451899"/>
    <dbReference type="KEGG" id="mja:MJ_1002"/>
    <dbReference type="eggNOG" id="arCOG04453">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063222_2_0_2"/>
    <dbReference type="InParanoid" id="Q58408"/>
    <dbReference type="OMA" id="GHESFIF"/>
    <dbReference type="OrthoDB" id="80301at2157"/>
    <dbReference type="PhylomeDB" id="Q58408"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019932">
        <property type="term" value="P:second-messenger-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00840">
        <property type="entry name" value="DacZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014499">
        <property type="entry name" value="DAC_DacZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019073">
        <property type="entry name" value="UCP019073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="SSF143597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107138" description="Diadenylate cyclase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="DAC" evidence="1">
        <location>
            <begin position="144"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="23812326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3">
        <source>
            <dbReference type="PubMed" id="25965978"/>
        </source>
    </evidence>
    <sequence length="309" mass="34380" checksum="382D44A261C76907" modified="1996-11-01" version="1">MIAKYIIKHGLELAYDIKADAFMIFTETGKSYELLKSFLKKDEHSGIIKILDKISHKNVKIIVATPNQVTYKKISSENEENIYPIFIKHREDNRCMIISSGIVHALKMKILKENNKIVAVVGEPKTPGKLDTIMVVNVKEHVKTITLYELFETLDEKQKRTLKEIIKLAMEIGREGREGEYVGTIFVMGDTLNVMSMSKPLILNPFAGHNASIFDENVKGTIKELSSIDGAFIITDEGKVVSAGRFLEIKGDVNIPKGLGARHLAAASISKNTNAIAVTVSQSGGIVRVFKDGKIVFETDPRANILFFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58422</accession>
    <name>Y1016_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1016</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1016</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64426">
        <property type="entry name" value="G64426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870529.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58422"/>
    <dbReference type="SMR" id="Q58422"/>
    <dbReference type="STRING" id="243232.MJ_1016"/>
    <dbReference type="DNASU" id="1451913"/>
    <dbReference type="EnsemblBacteria" id="AAB99022">
        <property type="protein sequence ID" value="AAB99022"/>
        <property type="gene ID" value="MJ_1016"/>
    </dbReference>
    <dbReference type="GeneID" id="1451913"/>
    <dbReference type="KEGG" id="mja:MJ_1016"/>
    <dbReference type="eggNOG" id="arCOG00044">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064237_0_0_2"/>
    <dbReference type="InParanoid" id="Q58422"/>
    <dbReference type="OMA" id="PYRMYEH"/>
    <dbReference type="OrthoDB" id="54396at2157"/>
    <dbReference type="PhylomeDB" id="Q58422"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012122">
        <property type="entry name" value="ATPase_PP-loop_MJ1016"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036670">
        <property type="entry name" value="ATPase_UCP036670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107142" description="Uncharacterized protein MJ1016">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <sequence length="294" mass="34015" checksum="7037DB82C69CD041" modified="1996-11-01" version="1">MEFSEWTKNKRKLNNLEELKRDIIEQFKEKNALDEGIVVMASGGKDSSTAIALAKDLGLNIEYLIHFYHRWSWDVSKKMVEKLSKKFDIPVIFYNITDELLKRTKGAKGSSICRICKNIMKDKAVDISKEKGIRIIMTGDSALEKVSGAVMNYLRDVYGEVVYNKMELTPVPQKYSKGKDKEVLFFRPLIRLACEDVLKLMDYYNIEIERAHEVGDKIGFWREGCCLQYADENALLNEKLFNELYKYNKIATEVAKKHGFRASIKLPSKKIMVVPKKDEYITLIKNALRDVDES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58438</accession>
    <name>Y1032_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1032</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1032</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To A.fulgidus AF2058.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64428">
        <property type="entry name" value="G64428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870545.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58438"/>
    <dbReference type="STRING" id="243232.MJ_1032"/>
    <dbReference type="EnsemblBacteria" id="AAB99036">
        <property type="protein sequence ID" value="AAB99036"/>
        <property type="gene ID" value="MJ_1032"/>
    </dbReference>
    <dbReference type="GeneID" id="1451929"/>
    <dbReference type="KEGG" id="mja:MJ_1032"/>
    <dbReference type="eggNOG" id="arCOG04151">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048986_1_0_2"/>
    <dbReference type="InParanoid" id="Q58438"/>
    <dbReference type="OMA" id="ESMYYTI"/>
    <dbReference type="OrthoDB" id="116265at2157"/>
    <dbReference type="PhylomeDB" id="Q58438"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="PTHR38815"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107149" description="Uncharacterized protein MJ1032">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="366" mass="41470" checksum="86D639A1A69BB9B2" modified="1996-11-01" version="1">MEKEGVKNYLVLVVDIDDDIGRKAGLNTPILGREENIKALIKLGLADPGDSDVNAILGGVKIYDELKASGKDVEIATISGDVDVESEKCALRIKEQIDFLLYLYNPDFIYLVSDGKEDEMILKYLESKNIFVWKKRVIVKQSETLESTYYLIQEFIKKTMEEYIPLILTFIGFSLILYAIFADIGWRIVVGIIGLYILSEGVGVRKLLMEKIKKKEEFDVGRVFPISASISIFILIIGLIYSLSSINKTSSTLTEFIGEFLLHFVDSLTLSLLILMVGKFVDTIINSEKDLLEILKKYFFCLICIFISRELIISGGEYLLKKISFIMFVMCVIIYISIVIILSVILFTKSSKEDKFKKLKNSITKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q58439</accession>
    <name>CORA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Cobalt/magnesium transport protein CorA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">corA</name>
        <name type="ordered locus">MJ1033</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="J. Bacteriol." volume="180" first="2788" last="2791">
            <title>Functional similarity between archaeal and bacterial CorA magnesium transporters.</title>
            <authorList>
                <person name="Smith R.L."/>
                <person name="Gottlieb E."/>
                <person name="Kucharski L.M."/>
                <person name="Maguire M.E."/>
            </authorList>
            <dbReference type="PubMed" id="9573171"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="J. Biol. Chem." volume="275" first="16767" last="16773">
            <title>Cation hexaammines are selective and potent inhibitors of the CorA magnesium transport system.</title>
            <authorList>
                <person name="Kucharski L.M."/>
                <person name="Lubbe W.J."/>
                <person name="Maguire M.E."/>
            </authorList>
            <dbReference type="PubMed" id="10748031"/>
            <dbReference type="DOI" id="10.1074/jbc.M001507200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>INHIBITION BY CATION HEXAAMMINES</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2004" name="J. Bacteriol." volume="186" first="4605" last="4612">
            <title>The CorA Mg2+ transporter is a homotetramer.</title>
            <authorList>
                <person name="Warren M.A."/>
                <person name="Kucharski L.M."/>
                <person name="Veenstra A."/>
                <person name="Shi L."/>
                <person name="Grulich P.F."/>
                <person name="Maguire M.E."/>
            </authorList>
            <dbReference type="PubMed" id="15231793"/>
            <dbReference type="DOI" id="10.1128/JB.186.14.4605-4612.2004"/>
        </citation>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2012" name="Proc. Natl. Acad. Sci. U.S.A." volume="109" first="18459" last="18464">
            <title>Structural insights into the mechanisms of Mg2+ uptake, transport, and gating by CorA.</title>
            <authorList>
                <person name="Guskov A."/>
                <person name="Nordin N."/>
                <person name="Reynaud A."/>
                <person name="Engman H."/>
                <person name="Lundback A.K."/>
                <person name="Jong A.J."/>
                <person name="Cornvik T."/>
                <person name="Phua T."/>
                <person name="Eshaghi S."/>
            </authorList>
            <dbReference type="PubMed" id="23091000"/>
            <dbReference type="DOI" id="10.1073/pnas.1210076109"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 1-317 IN COMPLEX WITH MAGNESIUM IONS</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>TOPOLOGY</scope>
        <scope>SUBUNIT</scope>
        <scope>MOTIF</scope>
        <scope>DOMAIN</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2015" name="Biochim. Biophys. Acta" volume="1848" first="2206" last="2215">
            <title>The Cryo-EM structure of the CorA channel from Methanocaldococcus jannaschii in low magnesium conditions.</title>
            <authorList>
                <person name="Cleverley R.M."/>
                <person name="Kean J."/>
                <person name="Shintre C.A."/>
                <person name="Baldock C."/>
                <person name="Derrick J.P."/>
                <person name="Ford R.C."/>
                <person name="Prince S.M."/>
            </authorList>
            <dbReference type="PubMed" id="26051127"/>
            <dbReference type="DOI" id="10.1016/J.BBAMEM.2015.06.002"/>
        </citation>
        <scope>STRUCTURE BY ELECTRON MICROSCOPY (21.60 ANGSTROMS)</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 6">Mediates influx of magnesium ions (PubMed:9573171, PubMed:10748031). Alternates between open and closed states. Activated by low cytoplasmic Mg(2+) levels. Inactive when cytoplasmic Mg(2+) levels are high. Can also mediate Co(2+) uptake.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by cation hexaammines.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3 4 5">Homopentamer (PubMed:23091000, PubMed:26051127). In the absence of Mg(2+), interactions between subunits are weakened, and dimers, trimers and tetramers can be observed in vitro (By similarity). Was initially proposed to be a homotetramer, based on the cross-linking studies (PubMed:15231793). This is in contradiction with current 3D-structures that clearly show it is a homopentamer (PubMed:23091000, PubMed:26051127).</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-16019330">
            <id>Q58439</id>
        </interactant>
        <interactant intactId="EBI-16019330">
            <id>Q58439</id>
            <label>corA</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8 10 11">Cell membrane</location>
            <topology evidence="4 5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4 5 7">The central ion permeation pathway is formed by the first transmembrane domain from each of the five subunits. Mg(2+) binding strengthens interactions between subunits and leads to the formation of a symmetrical homopentamer surrounding a closed ion permeation pathway (PubMed:23091000). Low cytoplasmic Mg(2+) concentrations trigger both a conformation change within each subunit and a loosening of the interactions between subunits. This results in an open ion conduction pathway. In addition, this results in a less symmetrical shape of the whole complex (PubMed:26051127).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64428">
        <property type="entry name" value="H64428"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870546.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4CY4">
        <property type="method" value="EM"/>
        <property type="resolution" value="21.60 A"/>
        <property type="chains" value="A/B/C/D/E=1-317"/>
    </dbReference>
    <dbReference type="PDB" id="4EGW">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-258"/>
    </dbReference>
    <dbReference type="PDB" id="4EV6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.20 A"/>
        <property type="chains" value="A/B/C/D/E=1-317"/>
    </dbReference>
    <dbReference type="PDBsum" id="4CY4"/>
    <dbReference type="PDBsum" id="4EGW"/>
    <dbReference type="PDBsum" id="4EV6"/>
    <dbReference type="AlphaFoldDB" id="Q58439"/>
    <dbReference type="SMR" id="Q58439"/>
    <dbReference type="DIP" id="DIP-60086N"/>
    <dbReference type="STRING" id="243232.MJ_1033"/>
    <dbReference type="TCDB" id="1.A.35.3.1">
        <property type="family name" value="the cora metal ion transporter (mit) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99037">
        <property type="protein sequence ID" value="AAB99037"/>
        <property type="gene ID" value="MJ_1033"/>
    </dbReference>
    <dbReference type="GeneID" id="1451930"/>
    <dbReference type="KEGG" id="mja:MJ_1033"/>
    <dbReference type="eggNOG" id="arCOG02265">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007127_0_0_2"/>
    <dbReference type="InParanoid" id="Q58439"/>
    <dbReference type="OMA" id="GILHTDH"/>
    <dbReference type="OrthoDB" id="43588at2157"/>
    <dbReference type="PhylomeDB" id="Q58439"/>
    <dbReference type="BRENDA" id="7.2.2.14">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004488">
        <property type="entry name" value="Mg/Co-transport_prot_CorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="SSF143865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="SSF144083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00383">
        <property type="entry name" value="corA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000201534" description="Cobalt/magnesium transport protein CorA">
        <location>
            <begin position="1"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="7">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="7">
        <location>
            <begin position="280"/>
            <end position="290"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
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    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="7">
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            <begin position="312"/>
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    </feature>
    <feature type="short sequence motif" description="Probable selectivity filter" evidence="9">
        <location>
            <begin position="278"/>
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        </location>
    </feature>
    <feature type="site" description="Essential for ion permeation" evidence="1">
        <location>
            <position position="254"/>
        </location>
    </feature>
    <feature type="site" description="Important for closing the ion permeation pathway in the closed state" evidence="4">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="site" description="Threonine that confers selectivity for Co(2+) transport" evidence="1">
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            <position position="261"/>
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    <feature type="strand" evidence="12">
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    <feature type="helix" evidence="12">
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        </source>
    </evidence>
    <evidence type="ECO:0007829" key="13">
        <source>
            <dbReference type="PDB" id="4EV6"/>
        </source>
    </evidence>
    <sequence length="317" mass="37142" checksum="552A0B3738131E0D" modified="1996-11-01" version="1">MITVIAIAKDGSIVEPKLDEISFEDYRLIWIDCYDPKDEELYKLSKKIGISVSDLQIGLDEQEIPRVEEDEDFYLIIYKAPLFEEDITTTSLGIYIKNNLLLTIHSDKIKAIGRLHKLISTKKPRIVFERGIGFLLYHILNEITRSYSRILMNLEDELEELEDKLLAGYDREVMEKILGLRKTLVYFHKSLIANRDVLVLLKRKYLPITTKEDRENFEDLYYDTLQLIDMSATYREVLTSMMDITLSLENIKMNQIMKILTMVTTIFAVPMWITGIYGMNFSYLPLANNPQGFWLVMALMVVIIMIFVYIFRRSGWI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q58445</accession>
    <name>RPO1N_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo1N</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit A'</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mja rpo1N intein</fullName>
            </recommendedName>
            <alternativeName>
                <fullName>Mja rpoA' intein</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Mja rpoA1 intein</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo1N</name>
        <name evidence="1" type="synonym">rpoA1</name>
        <name type="ordered locus">MJ1042</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds at least 2 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="2">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58445"/>
    <dbReference type="SMR" id="Q58445"/>
    <dbReference type="STRING" id="243232.MJ_1042"/>
    <dbReference type="PRIDE" id="Q58445"/>
    <dbReference type="EnsemblBacteria" id="AAB99046">
        <property type="protein sequence ID" value="AAB99046"/>
        <property type="gene ID" value="MJ_1042"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1042"/>
    <dbReference type="eggNOG" id="arCOG03158">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04257">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_258181_0_0_2"/>
    <dbReference type="InParanoid" id="Q58445"/>
    <dbReference type="OMA" id="AVCPPYN"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00863">
        <property type="entry name" value="RNApol_arch_Rpo1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012758">
        <property type="entry name" value="RPO1N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="PTHR19376"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000453784" description="DNA-directed RNA polymerase subunit Rpo1N">
        <location>
            <begin position="1"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000031062" description="DNA-directed RNA polymerase subunit Rpo1N, 1st part">
        <location>
            <begin position="1"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000031063" description="Mja rpo1N intein">
        <location>
            <begin position="461"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000031064" description="DNA-directed RNA polymerase subunit Rpo1N, 2nd part">
        <location>
            <begin position="912"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="918"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="920"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="922"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="1341" mass="152781" checksum="4D98546ED552AF12" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58492</accession>
    <name>Y1092_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative nickel/cobalt efflux system MJ1092</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1092</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Efflux system for nickel and cobalt.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NiCoT transporter (TC 2.A.52) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64436">
        <property type="entry name" value="C64436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870604.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58492"/>
    <dbReference type="STRING" id="243232.MJ_1092"/>
    <dbReference type="TCDB" id="2.A.113.1.4">
        <property type="family name" value="the nickel/cobalt transporter (nico) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99093">
        <property type="protein sequence ID" value="AAB99093"/>
        <property type="gene ID" value="MJ_1092"/>
    </dbReference>
    <dbReference type="GeneID" id="1451988"/>
    <dbReference type="KEGG" id="mja:MJ_1092"/>
    <dbReference type="eggNOG" id="arCOG03918">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058605_4_1_2"/>
    <dbReference type="InParanoid" id="Q58492"/>
    <dbReference type="OMA" id="HALEPGH"/>
    <dbReference type="OrthoDB" id="93540at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046583">
        <property type="term" value="F:cation efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015099">
        <property type="term" value="F:nickel cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035784">
        <property type="term" value="P:nickel cation homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032025">
        <property type="term" value="P:response to cobalt ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011541">
        <property type="entry name" value="Ni/Co_transpt_high_affinity"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03824">
        <property type="entry name" value="NicO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0171">Cobalt transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0921">Nickel transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000194015" description="Putative nickel/cobalt efflux system MJ1092">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="214" mass="22704" checksum="A21A318891B7891B" modified="1996-11-01" version="1">MVMIMELLYAITAFILGMLHALEPGHGKSVVAAYILGTKADLKDAILLGTTITISHTAVIFLLGILSIYLLESLNVDVVHDMMSVVGGLILIAVGIWIIRSYLHPHEHKVDTKKGVITLGLSAGLVPCPAALAVLLLSISSGNLIDGLIYVAIFSIGLAISLTGLAVAFVESKELIKKYVGNRKVSKLPLISGSIIILIGLYTIAHPILEHAIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q58546</accession>
    <name>G3P_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName>GAPDH</shortName>
            <ecNumber>1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">gap</name>
        <name type="ordered locus">MJ1146</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>D-glyceraldehyde 3-phosphate + NADP(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>D-glyceraldehyde 3-phosphate + NAD(+) + phosphate = (2R)-3-phospho-glyceroyl phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64443">
        <property type="entry name" value="A64443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870657.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2YYY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B=1-343"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YYY"/>
    <dbReference type="AlphaFoldDB" id="Q58546"/>
    <dbReference type="SMR" id="Q58546"/>
    <dbReference type="STRING" id="243232.MJ_1146"/>
    <dbReference type="EnsemblBacteria" id="AAB99147">
        <property type="protein sequence ID" value="AAB99147"/>
        <property type="gene ID" value="MJ_1146"/>
    </dbReference>
    <dbReference type="GeneID" id="1452042"/>
    <dbReference type="KEGG" id="mja:MJ_1146"/>
    <dbReference type="eggNOG" id="arCOG00493">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069533_0_0_2"/>
    <dbReference type="InParanoid" id="Q58546"/>
    <dbReference type="OMA" id="NAIVPNP"/>
    <dbReference type="OrthoDB" id="32079at2157"/>
    <dbReference type="PhylomeDB" id="Q58546"/>
    <dbReference type="BRENDA" id="1.2.1.59">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58546"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145723" description="Glyceraldehyde-3-phosphate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="198"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="3"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="51"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="83"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="93"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="101"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="120"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="137"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
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    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2YYY"/>
        </source>
    </evidence>
    <sequence length="343" mass="38102" checksum="81EB5810A9C838C5" modified="1996-11-01" version="1">MPAKVLINGYGSIGKRVADAVSMQDDMEVIGVTKTKPDFEARLAVEKGYKLFVAIPDNERVKLFEDAGIPVEGTILDIIEDADIVVDGAPKKIGKQNLENIYKPHKVKAILQGGEKAKDVEDNFNALWSYNRCYGKDYVRVVSCNTTGLCRILYAINSIADIKKARIVLVRRAADPNDDKTGPVNAITPNPVTVPSHHGPDVVSVVPEFEGKILTSAVIVPTTLMHMHTLMVEVDGDVSRDDILEAIKKTPRIITVRAEDGFSSTAKIIEYGRDLGRLRYDINELVVWEESINVLENEIFLMQAVHQESIVIPENIDCIRAMLQMEEDNFKSIEKTNKAMGIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q58576</accession>
    <name>PAN_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="1">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pan</name>
        <name type="ordered locus">MJ1176</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1680" last="1692">
            <title>Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome.</title>
            <authorList>
                <person name="Wilson H.L."/>
                <person name="Ou M.S."/>
                <person name="Aldrich H.C."/>
                <person name="Maupin-Furlow J."/>
            </authorList>
            <dbReference type="PubMed" id="10692374"/>
            <dbReference type="DOI" id="10.1128/JB.182.6.1680-1692.2000"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF N-TERMINUS</scope>
        <scope>FUNCTION AS AN ATPASE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH THE PROTEASOME</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1999" name="J. Biol. Chem." volume="274" first="26008" last="26014">
            <title>An archaebacterial ATPase, homologous to ATPases in the eukaryotic 26 S proteasome, activates protein breakdown by 20 S proteasomes.</title>
            <authorList>
                <person name="Zwickl P."/>
                <person name="Ng D."/>
                <person name="Woo K.M."/>
                <person name="Klenk H.-P."/>
                <person name="Goldberg A.L."/>
            </authorList>
            <dbReference type="PubMed" id="10473546"/>
            <dbReference type="DOI" id="10.1074/jbc.274.37.26008"/>
        </citation>
        <scope>FUNCTION IN THE PROTEASOME DEGRADATION PATHWAY</scope>
        <scope>ATPASE ACTIVITY</scope>
        <scope>NUCLEOTIDE SPECIFICITY</scope>
        <scope>ACTIVITY REGULATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="Nat. Cell Biol." volume="2" first="833" last="839">
            <title>PAN, the proteasome-activating nucleotidase from archaebacteria, is a protein-unfolding molecular chaperone.</title>
            <authorList>
                <person name="Benaroudj N."/>
                <person name="Goldberg A.L."/>
            </authorList>
            <dbReference type="PubMed" id="11056539"/>
            <dbReference type="DOI" id="10.1038/35041081"/>
        </citation>
        <scope>CHAPERONE ACTIVITY</scope>
    </reference>
    <reference key="5">
        <citation type="journal article" date="2005" name="Mol. Cell" volume="20" first="687" last="698">
            <title>ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins.</title>
            <authorList>
                <person name="Smith D.M."/>
                <person name="Kafri G."/>
                <person name="Cheng Y."/>
                <person name="Ng D."/>
                <person name="Walz T."/>
                <person name="Goldberg A.L."/>
            </authorList>
            <dbReference type="PubMed" id="16337593"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2005.10.019"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH THE PROTEASOME</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="6">
        <citation type="journal article" date="2007" name="Mol. Cell" volume="27" first="731" last="744">
            <title>Docking of the proteasomal ATPases' carboxyl termini in the 20S proteasome's alpha ring opens the gate for substrate entry.</title>
            <authorList>
                <person name="Smith D.M."/>
                <person name="Chang S.C."/>
                <person name="Park S."/>
                <person name="Finley D."/>
                <person name="Cheng Y."/>
                <person name="Goldberg A.L."/>
            </authorList>
            <dbReference type="PubMed" id="17803938"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2007.06.033"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>ROLE OF C-TERMINUS IN GATE OPENING</scope>
        <scope>MUTAGENESIS OF LEU-428; TYR-429 AND ARG-430</scope>
    </reference>
    <reference key="7">
        <citation type="journal article" date="2009" name="Mol. Cell" volume="34" first="485" last="496">
            <title>Mechanism of substrate unfolding and translocation by the regulatory particle of the proteasome from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Zhang F."/>
                <person name="Wu Z."/>
                <person name="Zhang P."/>
                <person name="Tian G."/>
                <person name="Finley D."/>
                <person name="Shi Y."/>
            </authorList>
            <dbReference type="PubMed" id="19481528"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2009.04.022"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>UNFOLDING AND TRANSLOCATION MECHANISM</scope>
        <scope>MUTAGENESIS OF GLY-113; ASP-153; ALA-156; LYS-157; PHE-244; ILE-245; GLY-246; 250-SER-LEU-251; GLU-271; GLY-285 AND GLY-286</scope>
    </reference>
    <reference key="8">
        <citation type="journal article" date="2009" name="Mol. Cell" volume="34" first="473" last="484">
            <title>Structural insights into the regulatory particle of the proteasome from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Zhang F."/>
                <person name="Hu M."/>
                <person name="Tian G."/>
                <person name="Zhang P."/>
                <person name="Finley D."/>
                <person name="Jeffrey P.D."/>
                <person name="Shi Y."/>
            </authorList>
            <dbReference type="PubMed" id="19481527"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2009.04.021"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 74-430 IN COMPLEX WITH ADP</scope>
        <scope>DOMAIN</scope>
    </reference>
    <reference key="9">
        <citation type="journal article" date="2010" name="EMBO J." volume="29" first="692" last="702">
            <title>Interactions of PAN's C-termini with archaeal 20S proteasome and implications for the eukaryotic proteasome-ATPase interactions.</title>
            <authorList>
                <person name="Yu Y."/>
                <person name="Smith D.M."/>
                <person name="Kim H.M."/>
                <person name="Rodriguez V."/>
                <person name="Goldberg A.L."/>
                <person name="Cheng Y."/>
            </authorList>
            <dbReference type="PubMed" id="20019667"/>
            <dbReference type="DOI" id="10.1038/emboj.2009.382"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 424-430 IN COMPLEX WITH THE PROTEASOME OF T.ACIDOPHILUM</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 4 5 7">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates. In addition to ATP, is able to cleave other nucleotide triphosphates such as CTP, GTP and UTP, but hydrolysis of these other nucleotides is less effective in promoting proteolysis than ATP. Moreover, PAN by itself can function as a chaperone in vitro.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2 3">ATPase activity is inhibited by EDTA, N-ethylmaleimide (NEM) and p-chloromercuriphenyl-sulfonic acid (PCMS) in vitro.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">497 uM for ATP</KM>
            <KM evidence="3">307 uM for CTP</KM>
            <Vmax evidence="3">3.5 umol/min/mg enzyme for ATPase activity</Vmax>
            <Vmax evidence="3">5.8 umol/min/mg enzyme for CTPase activity</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 7-8 for ATPase activity. Is more active at pH 8 to 10 than at pH 5.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 80 degrees Celsius for ATPase activity.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="3 4 6 8">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Alone, can form a complex composed of two stacked hexameric rings in vitro. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1 6">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB99179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="G64446"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="resolution" value="2.10 A"/>
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        <property type="entry name" value="AAA_lid_3"/>
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    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
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        <property type="entry name" value="ATPase_AAA_CS"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
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    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA_lid_3"/>
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        <property type="entry name" value="Prot_ATP_ID_OB"/>
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    </dbReference>
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        <property type="entry name" value="SSF52540"/>
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    </dbReference>
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        <property type="entry name" value="26Sp45"/>
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    </dbReference>
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    <keyword id="KW-0002">3D-structure</keyword>
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    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
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    <keyword id="KW-0547">Nucleotide-binding</keyword>
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    <keyword id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="428"/>
            <end position="430"/>
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    <feature type="coiled-coil region" evidence="1">
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    <feature type="binding site">
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    <feature type="binding site">
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            <position position="353"/>
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        <original>G</original>
        <variation>W</variation>
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            <position position="113"/>
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    <feature type="mutagenesis site" description="2% of wild-type unfolding activity." evidence="7">
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    <feature type="mutagenesis site" description="1.5% of wild-type unfolding activity." evidence="7">
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    <feature type="mutagenesis site" description="4% of wild-type unfolding activity." evidence="7">
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    <feature type="mutagenesis site" description="1% of wild-type unfolding activity." evidence="7">
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    <feature type="mutagenesis site" description="5% of wild-type unfolding activity." evidence="7">
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            <position position="246"/>
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    <feature type="mutagenesis site" description="4% of wild-type unfolding activity." evidence="7">
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            <begin position="250"/>
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            <position position="285"/>
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    <feature type="mutagenesis site" description="4% of wild-type unfolding activity." evidence="7">
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    <feature type="mutagenesis site" description="Markedly decreased PAN's ability to stimulate gate opening." evidence="5">
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    <feature type="mutagenesis site" description="Slightly decreased PAN's ability to stimulate gate opening." evidence="5">
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    <feature type="mutagenesis site" description="Loss of PAN's ability to stimulate gate opening. Fails to associate with the proteasome." evidence="5">
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        <variation>R</variation>
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            <position position="429"/>
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    <feature type="mutagenesis site" description="No effect on PAN's ability to stimulate gate opening. Still associates with the proteasome." evidence="5">
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    <feature type="mutagenesis site" description="Loss of PAN's ability to stimulate gate opening." evidence="5">
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            <position position="430"/>
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    <feature type="mutagenesis site" description="Slightly decreased PAN's ability to stimulate gate opening." evidence="5">
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            <position position="430"/>
        </location>
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    <feature type="mutagenesis site" description="Markedly decreased PAN's ability to stimulate gate opening." evidence="5">
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            <position position="430"/>
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    <feature type="mutagenesis site" description="Loss of PAN's ability to stimulate gate opening. Fails to associate with the proteasome." evidence="5">
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            <position position="430"/>
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    <feature type="mutagenesis site" description="Loss of PAN's ability to stimulate gate opening. Fails to associate with the proteasome." evidence="5">
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            <begin position="75"/>
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            <begin position="92"/>
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        <location>
            <begin position="113"/>
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            <begin position="126"/>
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            <begin position="134"/>
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            <begin position="144"/>
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            <begin position="159"/>
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            <begin position="169"/>
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    <feature type="helix" evidence="10">
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    <sequence length="430" mass="48690" checksum="3FD2E94A68D483DD" modified="1996-11-01" version="1">MVFEEFISTELKKEKKAFTEEFKEEKEINDNSNLKNDLLKEELQEKARIAELESRILKLELEKKELERENLQLMKENEILRRELDRMRVPPLIVGTVVDKVGERKVVVKSSTGPSFLVNVSHFVNPDDLAPGKRVCLNQQTLTVVDVLPENKDYRAKAMEVDERPNVRYEDIGGLEKQMQEIREVVELPLKHPELFEKVGIEPPKGILLYGPPGTGKTLLAKAVATETNATFIRVVGSELVKKFIGEGASLVKDIFKLAKEKAPSIIFIDEIDAIAAKRTDALTGGDREVQRTLMQLLAEMDGFDARGDVKIIGATNRPDILDPAILRPGRFDRIIEVPAPDEKGRLEILKIHTRKMNLAEDVNLEEIAKMTEGCVGAELKAICTEAGMNAIRELRDYVTMDDFRKAVEKIMEKKKVKVKEPAHLDVLYR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58580</accession>
    <name>Y1180_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ1180</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1180</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
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            <taxon>Methanocaldococcus</taxon>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
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    <dbReference type="EMBL" id="L77117">
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        <property type="evidence" value="ECO:0007669"/>
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            <begin position="4"/>
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</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58605</accession>
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            <fullName>S-adenosylmethionine synthase</fullName>
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            <fullName>Methionine adenosyltransferase</fullName>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
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            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Biol. Chem." volume="275" first="4055" last="4059">
            <title>Identification of a highly diverged class of S-adenosylmethionine synthetases in the archaea.</title>
            <authorList>
                <person name="Graham D.E."/>
                <person name="Bock C.L."/>
                <person name="Schalk-Hihi C."/>
                <person name="Lu Z.J."/>
                <person name="Markham G.D."/>
            </authorList>
            <dbReference type="PubMed" id="10660563"/>
            <dbReference type="DOI" id="10.1074/jbc.275.6.4055"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 3-43; 53-91; 132-178; 186-209; 213-225; 229-239; 250-269; 295-336 AND 347-379</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>PATHWAY</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O + L-methionine = diphosphate + phosphate + S-adenosyl-L-methionine</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64450">
        <property type="entry name" value="G64450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870720.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="7P82">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.04 A"/>
        <property type="chains" value="A/C=1-406"/>
    </dbReference>
    <dbReference type="PDB" id="7P83">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.22 A"/>
        <property type="chains" value="A/C=1-406"/>
    </dbReference>
    <dbReference type="PDB" id="7P84">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.05 A"/>
        <property type="chains" value="A/C=1-406"/>
    </dbReference>
    <dbReference type="PDB" id="7P8M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.71 A"/>
        <property type="chains" value="A/C=1-406"/>
    </dbReference>
    <dbReference type="PDBsum" id="7P82"/>
    <dbReference type="PDBsum" id="7P83"/>
    <dbReference type="PDBsum" id="7P84"/>
    <dbReference type="PDBsum" id="7P8M"/>
    <dbReference type="AlphaFoldDB" id="Q58605"/>
    <dbReference type="SMR" id="Q58605"/>
    <dbReference type="STRING" id="243232.MJ_1208"/>
    <dbReference type="EnsemblBacteria" id="AAB99212">
        <property type="protein sequence ID" value="AAB99212"/>
        <property type="gene ID" value="MJ_1208"/>
    </dbReference>
    <dbReference type="GeneID" id="1452105"/>
    <dbReference type="KEGG" id="mja:MJ_1208"/>
    <dbReference type="eggNOG" id="arCOG01678">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057642_0_0_2"/>
    <dbReference type="InParanoid" id="Q58605"/>
    <dbReference type="OMA" id="IGHPDSI"/>
    <dbReference type="OrthoDB" id="21658at2157"/>
    <dbReference type="PhylomeDB" id="Q58605"/>
    <dbReference type="BRENDA" id="2.5.1.6">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="PTHR36697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0554">One-carbon metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150028" description="S-adenosylmethionine synthase">
        <location>
            <begin position="1"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="141"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="4"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="14"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="17"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="59"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="84"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="95"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="99"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="109"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="133"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="145"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="155"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="162"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="166"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="190"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="224"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="230"/>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="255"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="turn" evidence="5">
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        </location>
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    <feature type="helix" evidence="5">
        <location>
            <begin position="319"/>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="340"/>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="359"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="392"/>
            <end position="399"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10660563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="7P82"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="7P8M"/>
        </source>
    </evidence>
    <sequence length="406" mass="45252" checksum="457B087EE1243404" modified="1996-11-01" version="1">MRNIIVKKLDVEPIEERPTEIVERKGLGHPDSICDGIAESVSRALCKMYMEKFGTILHHNTDQVELVGGHAYPKFGGGVMVSPIYILLSGRATMEILDKEKNEVIKLPVGTTAVKAAKEYLKKVLRNVDVDKDVIIDCRIGQGSMDLVDVFERQKNEVPLANDTSFGVGYAPLSTTERLVLETERFLNSDELKNEIPAVGEDIKVMGLREGKKITLTIAMAVVDRYVKNIEEYKEVIEKVRKKVEDLAKKIADGYEVEIHINTADDYERESVYLTVTGTSAEMGDDGSVGRGNRVNGLITPFRPMSMEAASGKNPVNHVGKIYNILANLIANDIAKLEGVKECYVRILSQIGKPINEPKALDIEIITEDSYDIKDIEPKAKEIANKWLDNIMEVQKMIVEGKVTTF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58614</accession>
    <name>Y1217_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ1217</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ1217</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1217</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable antitoxin component of a putative type VII toxin-antitoxin (TA) system. Neutralizes cognate toxic MJ1216 by di-AMPylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Probably forms a complex with cognate toxin MJ1216.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64451">
        <property type="entry name" value="H64451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870729.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58614"/>
    <dbReference type="SMR" id="Q58614"/>
    <dbReference type="STRING" id="243232.MJ_1217"/>
    <dbReference type="EnsemblBacteria" id="AAB99218">
        <property type="protein sequence ID" value="AAB99218"/>
        <property type="gene ID" value="MJ_1217"/>
    </dbReference>
    <dbReference type="GeneID" id="1452113"/>
    <dbReference type="KEGG" id="mja:MJ_1217"/>
    <dbReference type="eggNOG" id="arCOG01206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_10_3_2"/>
    <dbReference type="InParanoid" id="Q58614"/>
    <dbReference type="OMA" id="YVRQEQT"/>
    <dbReference type="OrthoDB" id="108083at2157"/>
    <dbReference type="PhylomeDB" id="Q58614"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107219" description="Putative protein adenylyltransferase MJ1217">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="98" mass="11559" checksum="074E10CA476F74D1" modified="1997-11-01" version="1">MKTLSEIKEILRKHKKILKEKYKVKSIAIFGSYAREEQKETSDIDILIDYYEPISLLKLIELENYLSDLLGIKVDLITKNSIHNPYVKKSIEEDLIYI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58648</accession>
    <name>Y1252_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1252</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1252</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0638 and MJ1123 and M.tuberculosis Rv2003c.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64456">
        <property type="entry name" value="B64456"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58648"/>
    <dbReference type="STRING" id="243232.MJ_1252"/>
    <dbReference type="EnsemblBacteria" id="AAB99255">
        <property type="protein sequence ID" value="AAB99255"/>
        <property type="gene ID" value="MJ_1252"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1252"/>
    <dbReference type="eggNOG" id="arCOG01774">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096708_0_0_2"/>
    <dbReference type="InParanoid" id="Q58648"/>
    <dbReference type="OMA" id="CLDMLYE"/>
    <dbReference type="PhylomeDB" id="Q58648"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107241" description="Uncharacterized protein MJ1252">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="251" mass="29161" checksum="BB12219F6E7201FF" modified="1996-11-01" version="1">MCKAFCSCYSYHYHHREKMGIKEYYDKLAKSYDKLYKNKYMRIVEREIIQKEIKDGDFVLDIGCGTGEQLKILNNAVGLDISLEMAKIAKNKTNKPVVVANAEFLPFKNKSFDKAISFFGALNHCNLKRALREVNRVLKDDGIFIFTVANIYDIKWIIKNILKGNFKKVKNAMKKRKGTITKVIDGEKIKVKTRFYDFKEVEDALKKEGFEVVYTFGTNITNSPLDKFIYKSFLKNFASYIGFVAKKVKNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58651</accession>
    <name>Y1254_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1254</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1254</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64456">
        <property type="entry name" value="E64456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870767.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58651"/>
    <dbReference type="SMR" id="Q58651"/>
    <dbReference type="STRING" id="243232.MJ_1254"/>
    <dbReference type="DNASU" id="1452152"/>
    <dbReference type="EnsemblBacteria" id="AAB99266">
        <property type="protein sequence ID" value="AAB99266"/>
        <property type="gene ID" value="MJ_1254"/>
    </dbReference>
    <dbReference type="GeneID" id="1452152"/>
    <dbReference type="KEGG" id="mja:MJ_1254"/>
    <dbReference type="eggNOG" id="arCOG00391">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637154_0_0_2"/>
    <dbReference type="OMA" id="QVYIIVP"/>
    <dbReference type="OrthoDB" id="52593at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107242" description="Uncharacterized protein MJ1254">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="469" mass="53926" checksum="E4FBDDCA1C1E3453" modified="1996-11-01" version="1">MIAFLFYKCFAKNYKINFLYKNSSYINTNTLWLKVIYIKTKDVIIFLLLFFVAFNVSSAYVIKNLNIDCIVTPDDTINETISFVIYNNEDKNLSHISYTIPQTIRNFTINASAGVKGYSALYNEGVTEIAIEFEKPIPKGGYTNITINCFVNDAIWTKNGIKQLILSFPITSKNATIKIVLPPGAVILSPQGTLLVTPSGYKITTDGKHQIIVWDLSLNKEITFTITVKYTFISYPGQNIIEQPAINNNLKYLLIIAIFGTAIFGGLFVKEKISKRKIIERTKNIKNELTSLKNKLKEKEEEIKNLAIKIKDLEDKLSKANKNLLNKDEIISVLNERISEYESQIQKLLDENIIYKEKIESLNKYIETLKKENDKLKDKVRELSDIAKKYMEEKRGVLWSFLTEDEKIIIDLIKKHGHITQKEIVEITGMSKPKVSRIISELEDRKIIRKEKIGRINKLTLTEESKKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58652</accession>
    <name>Y1255_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized glycosyltransferase MJ1255</fullName>
            <ecNumber>2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1255</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99267" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99267.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64456">
        <property type="entry name" value="F64456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496769.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58652"/>
    <dbReference type="SMR" id="Q58652"/>
    <dbReference type="STRING" id="243232.MJ_1255"/>
    <dbReference type="CAZy" id="GT1">
        <property type="family name" value="Glycosyltransferase Family 1"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99267">
        <property type="protein sequence ID" value="AAB99267"/>
        <property type="gene ID" value="MJ_1255"/>
    </dbReference>
    <dbReference type="GeneID" id="1452153"/>
    <dbReference type="KEGG" id="mja:MJ_1255"/>
    <dbReference type="eggNOG" id="arCOG01393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060247_0_0_2"/>
    <dbReference type="InParanoid" id="Q58652"/>
    <dbReference type="OMA" id="FGHTTRC"/>
    <dbReference type="OrthoDB" id="23719at2157"/>
    <dbReference type="PhylomeDB" id="Q58652"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005262">
        <property type="entry name" value="CHP00661"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00661">
        <property type="entry name" value="MJ1255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000215619" description="Uncharacterized glycosyltransferase MJ1255">
        <location>
            <begin position="1"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="394" mass="45637" checksum="9BAABAE2903328AD" modified="2003-06-06" version="2">MKILISVCGEGFGHTTRCVAIGEALKNDYEISYIAYGKSKNFIEKYGFKVFETFPEIKLKGKDGKFDITSSILNKEYSPKKAIRREINIIREYNPDLIISDCKYSTVVAAKLLKKPVICISNQNYTRYKLKTDLIVYPTMKALNIINERCERFIVPDFPLPYTICEYNLKIIKNMEFIGPLIRYDVDDVDNYGEDYILSVIGGFEYRYKILEELGKIALKNNLNVKLVCGSYEVAKKLMRDLNLNSYKNENVEIIPITTNMKELIKNAELIVSHGGHSTIMEALSFGKPLIVIPDLDHPEQGNNAKKVHDLGCGIALSYKELYRLEEAILDIRNMKMYKRNASKMKELAKKYDGKKNIKKIIDEFFETRKNIRKYYLTDRIANKLINKFKLKIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58670</accession>
    <name>DPHB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diphthine synthase</fullName>
            <ecNumber evidence="1">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dphB</name>
        <name type="ordered locus">MJ1274</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the diphthine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64459">
        <property type="entry name" value="A64459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870787.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58670"/>
    <dbReference type="SMR" id="Q58670"/>
    <dbReference type="STRING" id="243232.MJ_1274"/>
    <dbReference type="EnsemblBacteria" id="AAB99280">
        <property type="protein sequence ID" value="AAB99280"/>
        <property type="gene ID" value="MJ_1274"/>
    </dbReference>
    <dbReference type="GeneID" id="1452172"/>
    <dbReference type="KEGG" id="mja:MJ_1274"/>
    <dbReference type="eggNOG" id="arCOG04161">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_1_0_2"/>
    <dbReference type="InParanoid" id="Q58670"/>
    <dbReference type="OMA" id="TAGDPMV"/>
    <dbReference type="OrthoDB" id="102360at2157"/>
    <dbReference type="PhylomeDB" id="Q58670"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="PTHR10882"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156118" description="Diphthine synthase">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <sequence length="257" mass="29037" checksum="467B1CC2720108B7" modified="1996-11-01" version="1">MLILAGLGLYDENDMTLKTLKFAKKAEKIYAEFYTAVLTGTTTEKIEEVLGKKIHVLSRKDVEYNGYKLIEEAKDKDIMFLTAGDPMVATTHVDLAIEAKKKGIEVLIINAPSIYSAVGITGLQLYKFGKTTSIVFPEENYFPETPYNVIKENLERGLHTLCLLDIRIDENEKRFMTANEGLKVLLELENRKKEGIINEDTKAVVVARAGSLKPKLVYGKIKDLINYDFGEPLHCIIIPGKLHFMEEDALKYLCENI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58671</accession>
    <name>NAH3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable Na(+)/H(+) antiporter 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>MjNapA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1275</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="FEBS Lett." volume="527" first="245" last="249">
            <title>Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii.</title>
            <authorList>
                <person name="Hellmer J."/>
                <person name="Paetzold R."/>
                <person name="Zeilinger C."/>
            </authorList>
            <dbReference type="PubMed" id="12220668"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(02)03244-1"/>
        </citation>
        <scope>SIMILARITY TO NA(+)/H(+) ANTIPORTERS</scope>
    </reference>
    <comment type="function">
        <text>This is probably a Na(+)/H(+) antiporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64459">
        <property type="entry name" value="B64459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870788.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58671"/>
    <dbReference type="SMR" id="Q58671"/>
    <dbReference type="STRING" id="243232.MJ_1275"/>
    <dbReference type="EnsemblBacteria" id="AAB99281">
        <property type="protein sequence ID" value="AAB99281"/>
        <property type="gene ID" value="MJ_1275"/>
    </dbReference>
    <dbReference type="GeneID" id="1452173"/>
    <dbReference type="KEGG" id="mja:MJ_1275"/>
    <dbReference type="eggNOG" id="arCOG01953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005126_7_1_2"/>
    <dbReference type="InParanoid" id="Q58671"/>
    <dbReference type="OMA" id="EDKGDMQ"/>
    <dbReference type="OrthoDB" id="79669at2157"/>
    <dbReference type="PhylomeDB" id="Q58671"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0050">Antiport</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0739">Sodium transport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000052404" description="Probable Na(+)/H(+) antiporter 3">
        <location>
            <begin position="1"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="325"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="388" mass="42180" checksum="FF07E8CAEBA4F0CC" modified="1996-11-01" version="1">MESYYYVFFIILSIIFIVPNLLKKFNIPAITSIMIAGIIIGPYGLNILQVDETLKILADFGAIMLMFLAGLEVDNETLKQEFKNSLILSLFSLLIPGVGGYLIGQYLGLGFIGSLLYAVIFASHSVAIVYAILEELKMVKTRLGTIILSATIIVDLFTLLLLSVVIKLGIGGENVGTFLLETVLYIGVLLLAIPSLSKNILGVFEKLHAQRIHYVLFIIFIAIIVGEVIGIHPIVGAFICGVAVSEALTKEEHDELLNKNLNAIGYGFFIPIFFLVLGMETNIRVIFNLSNLELLLITLISAVALKFISGFIALRILGFDRIKNTIGGLLTVPKISASLVAASIGRELGLIGNEIFVTIVALSVITATITPIVVKHIFVAKCNKKAKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58683</accession>
    <name>Y1287_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1287</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1287</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64460">
        <property type="entry name" value="F64460"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58683"/>
    <dbReference type="SMR" id="Q58683"/>
    <dbReference type="STRING" id="243232.MJ_1287"/>
    <dbReference type="EnsemblBacteria" id="AAB99289">
        <property type="protein sequence ID" value="AAB99289"/>
        <property type="gene ID" value="MJ_1287"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1287"/>
    <dbReference type="eggNOG" id="arCOG01818">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_961776_0_0_2"/>
    <dbReference type="InParanoid" id="Q58683"/>
    <dbReference type="OMA" id="NVPPMLV"/>
    <dbReference type="PhylomeDB" id="Q58683"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000207311" description="Uncharacterized protein MJ1287">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="270" mass="31254" checksum="4DDE275588363C6B" modified="1996-11-01" version="1">MPTDLIRYGSISPEMLAYLWLLIEYKNSIMVAGEVATGKTTLLNAFSLFIPPQMKIVSIEDTPEIRLYHENWIAGTTRSGFGGEEYEITMMDLLKAALRQRPDYLIVGEVRGEEAKILFQAITTGHLALSTIHAKSPEAVIRRLNAEPMNIPKIMLEQLNAICMQVRLIYKGRFVRRTKSITEIVEYDPKIDDIILHDVFRWNPEDDTFEFSGESYLLRRIAEFIGISEKEIINELHSRAEFLRNLCKTKPNFEEFVKKICEYKEYHKGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-20" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58704</accession>
    <name>Y1308_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1308</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1308</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64463">
        <property type="entry name" value="C64463"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58704"/>
    <dbReference type="STRING" id="243232.MJ_1308"/>
    <dbReference type="EnsemblBacteria" id="AAB99328">
        <property type="protein sequence ID" value="AAB99328"/>
        <property type="gene ID" value="MJ_1308"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1308"/>
    <dbReference type="eggNOG" id="arCOG05076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271061_0_0_2"/>
    <dbReference type="OMA" id="YQLFWLS"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107266" description="Uncharacterized protein MJ1308">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="108" mass="12336" checksum="6EAD33F5583B8A09" modified="1996-11-01" version="1">MVDFMDYNDFQKKLDKEEHGDGITVGAVYTGEFTLYLLFIFGALIIGRVYGKTLMTLFGLAALAFSLSVSPLIFKFKEENSNAINYQLFWLSIFLGAIAFCIYMTTRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58706</accession>
    <name>Y1310_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1310</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1310</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64463">
        <property type="entry name" value="E64463"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58706"/>
    <dbReference type="SMR" id="Q58706"/>
    <dbReference type="STRING" id="243232.MJ_1310"/>
    <dbReference type="EnsemblBacteria" id="AAB99317">
        <property type="protein sequence ID" value="AAB99317"/>
        <property type="gene ID" value="MJ_1310"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1310"/>
    <dbReference type="eggNOG" id="arCOG03072">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082058_2_1_2"/>
    <dbReference type="InParanoid" id="Q58706"/>
    <dbReference type="OMA" id="KRHGTVD"/>
    <dbReference type="PhylomeDB" id="Q58706"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107268" description="Uncharacterized protein MJ1310">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="128" mass="13938" checksum="8A7166717236008C" modified="1996-11-01" version="1">MIMINYLVNRMDFQMASFITSGLLVIIGLYGVFFVDNVLKKIIALEILGSGVNLALIAIGYNGGTIPIKLPGVSVEVFAKESAYPLTHALVLTNIVIEASMLAVMLGVSIILYKKYKTLRSSVILKED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58721</accession>
    <name>Y1325_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ1325</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1325</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64465">
        <property type="entry name" value="D64465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870842.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58721"/>
    <dbReference type="SMR" id="Q58721"/>
    <dbReference type="STRING" id="243232.MJ_1325"/>
    <dbReference type="EnsemblBacteria" id="AAB99335">
        <property type="protein sequence ID" value="AAB99335"/>
        <property type="gene ID" value="MJ_1325"/>
    </dbReference>
    <dbReference type="GeneID" id="8512529"/>
    <dbReference type="KEGG" id="mja:MJ_1325"/>
    <dbReference type="eggNOG" id="arCOG01680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097806_3_5_2"/>
    <dbReference type="InParanoid" id="Q58721"/>
    <dbReference type="OMA" id="MCVCELE"/>
    <dbReference type="OrthoDB" id="111287at2157"/>
    <dbReference type="PhylomeDB" id="Q58721"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000160634" description="Uncharacterized HTH-type transcriptional regulator MJ1325">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00340"/>
        </source>
    </evidence>
    <sequence length="89" mass="10292" checksum="33AFC876DCD861F9" modified="1996-11-01" version="1">MEKYEKAAEIFKAFGDPTRLMILKLLAENGSMCVCKIIDELKKPQPTISHHLNILKKAGIVKARKEGTWNFYYIVDDRVKEIIKLVDEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58730</accession>
    <name>Y1334_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber>2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Alpha-D-glucosyl-1-phosphate uridylyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>UDP-glucose pyrophosphorylase</fullName>
            <shortName>UDPGP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Uridine diphosphoglucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1334</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>alpha-D-glucose 1-phosphate + H(+) + UTP = diphosphate + UDP-alpha-D-glucose</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64466">
        <property type="entry name" value="E64466"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870852.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58730"/>
    <dbReference type="SMR" id="Q58730"/>
    <dbReference type="STRING" id="243232.MJ_1334"/>
    <dbReference type="EnsemblBacteria" id="AAB99341">
        <property type="protein sequence ID" value="AAB99341"/>
        <property type="gene ID" value="MJ_1334"/>
    </dbReference>
    <dbReference type="GeneID" id="1452237"/>
    <dbReference type="KEGG" id="mja:MJ_1334"/>
    <dbReference type="eggNOG" id="arCOG00665">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029499_1_2_2"/>
    <dbReference type="InParanoid" id="Q58730"/>
    <dbReference type="OMA" id="MHYVRQG"/>
    <dbReference type="OrthoDB" id="78828at2157"/>
    <dbReference type="PhylomeDB" id="Q58730"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009058">
        <property type="term" value="P:biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="PTHR43197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000201377" description="Putative UTP--glucose-1-phosphate uridylyltransferase">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="283" mass="32090" checksum="A98C684C9940F127" modified="1996-11-01" version="1">MIRKAVIPVAGFGTRLLPITKAQPKEMLPVVNKPIVQYVVEDLVEAGVKDILFVTGKGKQAIENHFDVNYELECKLEKSGKYELLKIIKEIDRLGNIFYVRQKEQKGLGDAILYGEEFVGEEYFIAMVGDTIYSKNIVKDLIKAHEKYGCSVIALERVPKEDVYKYGVIDGEEIEKGVYKIKNMVEKPKVEEAPSNLIITGAYLLSPKIFEKIRETPPGRGGEIQITDAMNLLLKEEDIIGVEINCKRYDIGDALGWLKANVEIGAERFPEFREFLKEFVKNL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58763</accession>
    <name>WTPB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Molybdate/tungstate transport system permease protein WtpB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">wtpB</name>
        <name type="ordered locus">MJ1368</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64470">
        <property type="entry name" value="G64470"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58763"/>
    <dbReference type="SMR" id="Q58763"/>
    <dbReference type="STRING" id="243232.MJ_1368"/>
    <dbReference type="TCDB" id="3.A.1.6.9">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99376">
        <property type="protein sequence ID" value="AAB99376"/>
        <property type="gene ID" value="MJ_1368"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1368"/>
    <dbReference type="eggNOG" id="arCOG00164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016047_14_1_2"/>
    <dbReference type="InParanoid" id="Q58763"/>
    <dbReference type="OMA" id="NEYNFVA"/>
    <dbReference type="PhylomeDB" id="Q58763"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0500">Molybdenum</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060308" description="Molybdate/tungstate transport system permease protein WtpB">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="115"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="138"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="245"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="3">
        <location>
            <begin position="45"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="249" mass="27942" checksum="3359BC2A3EB48675" modified="1996-11-01" version="1">MEKFDIAMTVFLVMIFLFIFLPIIYMLSNPGDLNQLLDKEVIEAFKTTLLAGAVATLIALIFGIPTGYILARYDFKFKSFVEAVLDLPMAIPHSVIGIIILSFIYGIDIINFIGRYVVDNFWGIVTVYLFVGIPFMVNSIRDGFLSVDEEIEYVSRTLGASKIRTFFEISLPLIKNNIISGIILSFARGISEVGAILIIAYYPKTVPILIYERFMSFGLDASKPISVGMILISIALFALLRMFGRMRGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58767</accession>
    <name>UPPS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="1">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="1">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="1">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">uppS</name>
        <name type="ordered locus">MJ1372</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tri-trans,hepta-cis-undecaprenyl diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64471">
        <property type="entry name" value="C64471"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58767"/>
    <dbReference type="SMR" id="Q58767"/>
    <dbReference type="STRING" id="243232.MJ_1372"/>
    <dbReference type="PRIDE" id="Q58767"/>
    <dbReference type="EnsemblBacteria" id="AAB99380">
        <property type="protein sequence ID" value="AAB99380"/>
        <property type="gene ID" value="MJ_1372"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1372"/>
    <dbReference type="eggNOG" id="arCOG01532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038505_2_0_2"/>
    <dbReference type="InParanoid" id="Q58767"/>
    <dbReference type="OMA" id="PRTEGHK"/>
    <dbReference type="PhylomeDB" id="Q58767"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002094">
        <property type="term" value="F:polyprenyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="PTHR10291"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="SSF64005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123731" description="Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)">
        <location>
            <begin position="1"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="58"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="235"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <sequence length="280" mass="33286" checksum="FCB6C9FF5C65CC76" modified="1997-11-01" version="1">MGILGKIKNKLKSIGKRVIIDFYRFLDNSGVLKIYEKILEEAIDKDNLPKHVAIIMDGNRRAAEIYGKDRYYGHYLGAEKVREVLRWARDLGINVVTLYAFSTENFRRPKEEVDKLMELFEKKFYEIADDEEIHRYEVRVRAIGRINLLPKNVQKAIKYAEERTKNYNKFFVNIAIAYGGQQEIIDAVKKIAEKVKRGEIEPEDIDKELIDKHLYTANLPFPNPDLIIRTSGEERISNFLIWQSSYSELYFCDIYWPLFRRVDFLRAVRDYQRRQRRFGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58778</accession>
    <name>Y1383_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1383</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1383</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64472">
        <property type="entry name" value="F64472"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870900.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58778"/>
    <dbReference type="SMR" id="Q58778"/>
    <dbReference type="STRING" id="243232.MJ_1383"/>
    <dbReference type="DNASU" id="1452286"/>
    <dbReference type="EnsemblBacteria" id="AAB99393">
        <property type="protein sequence ID" value="AAB99393"/>
        <property type="gene ID" value="MJ_1383"/>
    </dbReference>
    <dbReference type="GeneID" id="1452286"/>
    <dbReference type="KEGG" id="mja:MJ_1383"/>
    <dbReference type="eggNOG" id="arCOG00892">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061552_1_0_2"/>
    <dbReference type="InParanoid" id="Q58778"/>
    <dbReference type="OMA" id="WAAFTIY"/>
    <dbReference type="OrthoDB" id="44748at2157"/>
    <dbReference type="PhylomeDB" id="Q58778"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="PTHR42658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014013" description="Uncharacterized protein MJ1383">
        <location>
            <begin position="31"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="256" mass="29036" checksum="6D26F427EAB54675" modified="1996-11-01" version="1" precursor="true">MIDTHIHSDTRGLEDLELMAMCLDGVITLAHDPFEMKNIKVWEAHVEKLLINELERAKKVGLNLFICVGMHPRAIPPEIDEALDKIKSYINYNSRVVGIGEIGLEKATKEEKEVFIKQLLLAEELNMPAVVHTPRRNKEEVTKIILDEISTLNLKNRDIVIEHCNKETTKWVLDEEFYVGLTIQPGKLTPLEAVEIVKEYKDFADKILLNSDCSSNASDVLAVPRTVLKMKINGIEKDVIYKVAHKNAVNLFGLDI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58821</accession>
    <name>Y1426_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1426</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1426</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64478">
        <property type="entry name" value="A64478"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58821"/>
    <dbReference type="SMR" id="Q58821"/>
    <dbReference type="STRING" id="243232.MJ_1426"/>
    <dbReference type="EnsemblBacteria" id="AAB99437">
        <property type="protein sequence ID" value="AAB99437"/>
        <property type="gene ID" value="MJ_1426"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1426"/>
    <dbReference type="eggNOG" id="arCOG00606">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040681_9_0_2"/>
    <dbReference type="InParanoid" id="Q58821"/>
    <dbReference type="OMA" id="WDKQVHQ"/>
    <dbReference type="PhylomeDB" id="Q58821"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000107319" description="Uncharacterized protein MJ1426">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="20"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="117"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <sequence length="168" mass="18874" checksum="690A7A4B7902B300" modified="1996-11-01" version="1">MRTILNKLNINGEIMLIKDIMKKPIVVYEDNDLIDVIRLFRKNKISGAPVLNKDGKLVGIISESDIVKTIVTHNEDLNLILPSPLDLIELPLKTALKIEEFMEDLKNALKTKVRDVMTRKVIVAKPDMTINDAAKLMVKNNIKRLPVVDDEGNLIGIVTRGDLIEALI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58824</accession>
    <name>HERA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable DNA double-strand break repair helicase HerA</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">herA</name>
        <name evidence="5" type="ordered locus">MJ1429</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Involved in DNA double-strand break (DSB) repair (By similarity). Acts probably with NurA to stimulate resection of the 5' strand and produce the long 3' single-strand that is required for RadA loading (By similarity). Exhibits DNA-dependent ATPase activity and DNA helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64478">
        <property type="entry name" value="D64478"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58824"/>
    <dbReference type="SMR" id="Q58824"/>
    <dbReference type="STRING" id="243232.MJ_1429"/>
    <dbReference type="EnsemblBacteria" id="AAB99439">
        <property type="protein sequence ID" value="AAB99439"/>
        <property type="gene ID" value="MJ_1429"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1429"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="InParanoid" id="Q58824"/>
    <dbReference type="OMA" id="EPNDQRY"/>
    <dbReference type="PhylomeDB" id="Q58824"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033186">
        <property type="entry name" value="HerA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="PTHR42957"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05872">
        <property type="entry name" value="DUF853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107323" description="Probable DNA double-strand break repair helicase HerA">
        <location>
            <begin position="1"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="131"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="478"/>
            <end position="479"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="F2Z5Z6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8U1P0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q97WG8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000312" key="5">
        <source>
            <dbReference type="EMBL" id="AAB99439.1"/>
        </source>
    </evidence>
    <sequence length="503" mass="56697" checksum="BBDADC0E52849657" modified="1997-11-01" version="1">MYVMKVVGKTTTTHFTFESLEKIRFGEYVIAKNVDGRDVLGVIKNVVADVEKFVGEVKVIGVLDGNKIIPNRTPILPNSEVRLCDDEILNNIYLTPDGLNIGHLLTRDNVRVYLDTNKLVSRHFAILSITGGGKSNTASVLCRELAKKNGTVIMIDPHGEYISLYHEDMEGKIKVINPIINPVLLAPSEFANLIGIGDNEIEKRVYVEFAYHTVKHECPDAKGIEFIEKIENLLYEWSKIASVGWEIKYYNPLRRNYDRRKLEKEDFVILMSLIDTISKFKLDYALNIGDRDVIEEFEIGKINIVNLSGLEIPQMVTFVGFIAKHLLLKRITYLKSLKDVYSINEEIRRVAQSNLNIIESHYKVVTKPVLLIVEEAHIFIPVNEQNSASLWLGKIAREGRKFGVGLGLVSQRPKQLHPDVLSQTNTKIILKIVEPEDQKYIQRASEELGEDLVKDLASLGIGEAVIVGAAISLPSIVKIDKFDGVYGGKDINIVGEWMGLDDW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58838</accession>
    <name>Y1443_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0132 membrane protein MJ1443</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1443</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0132 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64480">
        <property type="entry name" value="B64480"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58838"/>
    <dbReference type="STRING" id="243232.MJ_1443"/>
    <dbReference type="EnsemblBacteria" id="AAB99453">
        <property type="protein sequence ID" value="AAB99453"/>
        <property type="gene ID" value="MJ_1443"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1443"/>
    <dbReference type="eggNOG" id="arCOG04344">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095018_3_0_2"/>
    <dbReference type="InParanoid" id="Q58838"/>
    <dbReference type="OMA" id="FVKFHAM"/>
    <dbReference type="PhylomeDB" id="Q58838"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019109">
        <property type="entry name" value="DUF4870"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09685">
        <property type="entry name" value="DUF4870"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000158602" description="UPF0132 membrane protein MJ1443">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="110" mass="12411" checksum="0D162443D7741796" modified="1996-11-01" version="1">MIFMGKTSLGLDENIEGALCYLFGVITGILFYILEKESKFVKFHAVQSIILFGGLWVLSIILAFIPYGWMLSGLVNLAAFILWIVCMYKAYKGEKFKLPVIGDIAEQYSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58880</accession>
    <name>Y1485_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized cation transporter MJ1485</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1485</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64485">
        <property type="entry name" value="D64485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871007.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58880"/>
    <dbReference type="SMR" id="Q58880"/>
    <dbReference type="STRING" id="243232.MJ_1485"/>
    <dbReference type="EnsemblBacteria" id="AAB99495">
        <property type="protein sequence ID" value="AAB99495"/>
        <property type="gene ID" value="MJ_1485"/>
    </dbReference>
    <dbReference type="GeneID" id="1452391"/>
    <dbReference type="KEGG" id="mja:MJ_1485"/>
    <dbReference type="eggNOG" id="arCOG04145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030708_3_0_2"/>
    <dbReference type="InParanoid" id="Q58880"/>
    <dbReference type="OMA" id="LQWMGGM"/>
    <dbReference type="OrthoDB" id="13996at2157"/>
    <dbReference type="PhylomeDB" id="Q58880"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000070483" description="Uncharacterized cation transporter MJ1485">
        <location>
            <begin position="1"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="474" mass="52537" checksum="C78608E4645BE7A2" modified="1996-11-01" version="1">MGICRLTKKDIEGILHILGGIIQIIGIFTLVPCIVSVYYNENTFLNFLIPGLFFSIFGFVLKRATKPKNLKLHHTMVASALAWLIASFIGAIPLYLSIDYFSYVDAVYESMSAWTTTGMTLIPNVEVLPKSILFWRSFQQWIGGVGILVLSALVLARSGTVAYLLYTSEARQERIMPSAIGTIKTIIWIYILYTILGVLLLYLSGLSFWDALNLTMTGISTGGMSISNYSFPYNDFAKIVMIGIMMVGGVMSFSIHHKLLTGKYFNDIQTKYALIVTAFISIIISIKDKVPIIDSLFTVVSAMTSTGFTTINVGNLSSLSLFLIIFLMLIGGGAGTTTGGVKIIRFLVILKALLYEIKEIIYPKSAVIHEHLDDMDLNYRIIREAFVVFFLYCLSSFLTALIFIALGYNPYDSIFDAVSFTSNIGISLGVVTLKTPVIGKIAGIIAMWIGRLEIIPVLVLFATLYFKTLRLLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-03" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58892</accession>
    <name>Y1497_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized metallohydrolase MJ1497</fullName>
            <ecNumber>3.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1497</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) or Co(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EC" id="3.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64486">
        <property type="entry name" value="H64486"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871020.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58892"/>
    <dbReference type="SMR" id="Q58892"/>
    <dbReference type="STRING" id="243232.MJ_1497"/>
    <dbReference type="EnsemblBacteria" id="AAB99508">
        <property type="protein sequence ID" value="AAB99508"/>
        <property type="gene ID" value="MJ_1497"/>
    </dbReference>
    <dbReference type="GeneID" id="1452404"/>
    <dbReference type="KEGG" id="mja:MJ_1497"/>
    <dbReference type="eggNOG" id="arCOG01107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_800794_0_0_2"/>
    <dbReference type="InParanoid" id="Q58892"/>
    <dbReference type="OMA" id="VSGHKGM"/>
    <dbReference type="OrthoDB" id="87784at2157"/>
    <dbReference type="PhylomeDB" id="Q58892"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="SSF55031"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000185353" description="Uncharacterized metallohydrolase MJ1497">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="346" mass="39800" checksum="97E0E7B0DE358CFA" modified="1996-11-01" version="1">MIIMDYLKILEDLVKIRTDNRIGVKKAFKYLSNLFNNLGIKNTIIEGCFVAYKEKENFDLILNSHIDTVKIQSNFKKDDNNFYGTGVIDAKGNVVLMIHAFLNSNNSLLVISPDEETESNGIYNFCQYLRNKNKIQRGIKCIVGEPTDLNVCIGHKGRFEYIVESFGEARHASSQGLNPIEILSRVILDLKNLPLEKIKVDKIYSSSITPTIIKGGIQSNIIPDYAYVLFDVRSVEKDIIKKIDDFLSQKNYSKHIKSSLNPERHYANFYMLENKELINKLSKHFKISFFNATCEAYYFNKFLKADTIIYGVGKLELAHSKEEYLNLNDFDRGIKEVEKLAELMIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58910</accession>
    <name>Y1515_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1515</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1515</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="B64489">
        <property type="entry name" value="B64489"/>
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    <dbReference type="RefSeq" id="WP_010871038.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="STRING" id="243232.MJ_1515"/>
    <dbReference type="EnsemblBacteria" id="AAB99533">
        <property type="protein sequence ID" value="AAB99533"/>
        <property type="gene ID" value="MJ_1515"/>
    </dbReference>
    <dbReference type="GeneID" id="1452422"/>
    <dbReference type="KEGG" id="mja:MJ_1515"/>
    <dbReference type="eggNOG" id="arCOG03639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG07614">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059273_0_1_2"/>
    <dbReference type="InParanoid" id="Q58910"/>
    <dbReference type="OMA" id="YWVFAHN"/>
    <dbReference type="OrthoDB" id="44166at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
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        <property type="entry name" value="GATase_2_dom"/>
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    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="GATase_4"/>
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        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000107386" description="Uncharacterized protein MJ1515">
        <location>
            <begin position="2"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00609"/>
        </source>
    </evidence>
    <sequence length="355" mass="41888" checksum="55921898CC90E304" modified="1996-11-01" version="1">MCELLGICFNKKVNVELSLNSFKHRSEDHPNGWGIAFYPDGFVRVIKEPIKMNEALLAECVRWTKIKSNIFIAHIRKASAGSESYVNTHPFVRKLEDKEIAFAHNGTLLGYEDLELDGYYPIGETDSEYVFCYLLSQIEKREIEWNKEGFDEMLDILLDINYYGAFNCLFSDGEYLFAYKDYRGRRELHFLKRKPPYGRIRLEDEDYIINLGDVKSVREEGFIIATNPLTNEDWKSFENGELMVFKNGEMIYSNKRLTDLELKILKILRESPHRVSLKKIIENLEYLSRNIRHDASVVRIGIRSLLDKGYIKQDSRDTVNWDDLEATFYTKPEKRVEIDKRLKGFRWRRNRIIMS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58933</accession>
    <name>PSTK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>L-seryl-tRNA(Sec) kinase</fullName>
            <ecNumber>2.7.1.164</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>O-phosphoseryl-tRNA(Sec) kinase</fullName>
            <shortName>PSTK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pstK</name>
        <name type="ordered locus">MJ1538</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Biochemistry" volume="44" first="13315" last="13327">
            <title>Structural and functional investigation of a putative archaeal selenocysteine synthase.</title>
            <authorList>
                <person name="Kaiser J.T."/>
                <person name="Gromadski K."/>
                <person name="Rother M."/>
                <person name="Engelhardt H."/>
                <person name="Rodnina M.V."/>
                <person name="Wahl M.C."/>
            </authorList>
            <dbReference type="PubMed" id="16201757"/>
            <dbReference type="DOI" id="10.1021/bi051110r"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Specifically phosphorylates seryl-tRNA(Sec) to O-phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-seryl-tRNA(Sec) = ADP + O-phospho-L-seryl-tRNA(Sec)</text>
            <dbReference type="Rhea" id="RHEA:25037"/>
            <dbReference type="Rhea" id="RHEA-COMP:9742"/>
            <dbReference type="Rhea" id="RHEA-COMP:9947"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:78533"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.164"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the L-seryl-tRNA(Sec) kinase family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99557" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.7.1.164"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99557.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64492">
        <property type="entry name" value="A64492"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496861.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3A4L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="3A4M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.79 A"/>
        <property type="chains" value="A/B=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="3A4N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-248"/>
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    <dbReference type="PDB" id="3ADB">
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        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="3ADC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="3ADD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-248"/>
    </dbReference>
    <dbReference type="PDB" id="3AM1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A=1-248"/>
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    <dbReference type="PDBsum" id="3A4L"/>
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    <dbReference type="PDBsum" id="3ADD"/>
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    <dbReference type="SMR" id="Q58933"/>
    <dbReference type="DIP" id="DIP-48971N"/>
    <dbReference type="STRING" id="243232.MJ_1538"/>
    <dbReference type="PRIDE" id="Q58933"/>
    <dbReference type="EnsemblBacteria" id="AAB99557">
        <property type="protein sequence ID" value="AAB99557"/>
        <property type="gene ID" value="MJ_1538"/>
    </dbReference>
    <dbReference type="GeneID" id="1452446"/>
    <dbReference type="KEGG" id="mja:MJ_1538"/>
    <dbReference type="eggNOG" id="arCOG01041">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1100964_0_0_2"/>
    <dbReference type="InParanoid" id="Q58933"/>
    <dbReference type="OMA" id="RESFPVW"/>
    <dbReference type="OrthoDB" id="67618at2157"/>
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    <dbReference type="BioCyc" id="MetaCyc:MON-14956"/>
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        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00897"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58933"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043915">
        <property type="term" value="F:L-seryl-tRNA(Sec) kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097056">
        <property type="term" value="P:selenocysteinyl-tRNA(Sec) biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020024">
        <property type="entry name" value="L-seryl-tRNA_Sec_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03574">
        <property type="entry name" value="selen_PSTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107396" description="L-seryl-tRNA(Sec) kinase">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="13"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="31"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="37"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="48"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="69"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="96"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="145"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="209"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="229"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="234"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16201757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3A4M"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3ADC"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="3ADD"/>
        </source>
    </evidence>
    <sequence length="248" mass="29467" checksum="815EF9A453BEA450" modified="2007-05-01" version="2">MLIILTGLPGVGKSTFSKNLAKILSKNNIDVIVLGSDLIRESFPVWKEKYEEFIKKSTYRLIDSALKNYWVIVDDTNYYNSMRRDLINIAKKYNKNYAIIYLKASLDVLIRRNIERGEKIPNEVIKKMYEKFDEPGKKYKWDEPFLIIDTTKDIDFNEIAKKLIEKSKEIPKFYVLEENKNKNNNISDKIDKETRKIVSEYIKSKKLDKDKIKEVVELRKEFLKKIKKMEEVDADRVLKEFKDLLNSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58934</accession>
    <name>Y1539_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1539</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1539</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To A.fulgidus AF1885.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64492">
        <property type="entry name" value="B64492"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58934"/>
    <dbReference type="SMR" id="Q58934"/>
    <dbReference type="STRING" id="243232.MJ_1539"/>
    <dbReference type="EnsemblBacteria" id="AAB99568">
        <property type="protein sequence ID" value="AAB99568"/>
        <property type="gene ID" value="MJ_1539"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1539"/>
    <dbReference type="eggNOG" id="arCOG08297">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230419_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107397" description="Uncharacterized protein MJ1539">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="109" mass="12451" checksum="C0E04280C5960831" modified="1996-11-01" version="1">MGIDMSEEKEVKEIKVEIPKEIADKIEKITSFLNSLKDKKEMVSKEKIEEMLKLIDEVKDKLPKLNVDIEKLAKILEGSGSEVKIVFNKLTIDGEVGLKIIPLKKEKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58955</accession>
    <name>Y1560_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized MFS-type transporter MJ1560</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1560</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64494">
        <property type="entry name" value="G64494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871084.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58955"/>
    <dbReference type="SMR" id="Q58955"/>
    <dbReference type="STRING" id="243232.MJ_1560"/>
    <dbReference type="EnsemblBacteria" id="AAB99579">
        <property type="protein sequence ID" value="AAB99579"/>
        <property type="gene ID" value="MJ_1560"/>
    </dbReference>
    <dbReference type="GeneID" id="1452468"/>
    <dbReference type="KEGG" id="mja:MJ_1560"/>
    <dbReference type="eggNOG" id="arCOG00130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_14_2"/>
    <dbReference type="InParanoid" id="Q58955"/>
    <dbReference type="OMA" id="LYADSFH"/>
    <dbReference type="OrthoDB" id="22281at2157"/>
    <dbReference type="PhylomeDB" id="Q58955"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="SSF103473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000084887" description="Uncharacterized MFS-type transporter MJ1560">
        <location>
            <begin position="1"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="386" mass="42068" checksum="2B928E8E56D1BFC1" modified="1996-11-01" version="1">MGKLEKNVFVIWITTFTTMLGVGFIAPIMAIYAQTLGATNLEIGLIFGSFALARTVAQIPVGVLSDIYGKKFFIVCGTFFYGVSTLMYNFVSTVLGFLIVRIFTGIFSAFVTPVAGSYIAAIAPKTRLGEYMGIFNSAITLGFGIGPFIGGILADMYGIKMPFYFCGFLGILAAIISYMKLEDIVFNKNKEKIDVKKISTLFSFEFLKNRNFSSSFIINVSNVMINAGIYAYLALYAINYNITISQVGFMIALTNILMALLQRSFGKLYDKLGNIMIIIGIFIISFGMYLLSTSTTFLTILASLTIIAVGSSISSTATTSLAVKDIPTHRKGEAMGLFTTSINIGMFIGAVSFGFLADILGIANMYKFSAIFSIVVGIISYLRIER</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q59004</accession>
    <name>Y1609_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ1609</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1609</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64500">
        <property type="entry name" value="H64500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871134.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59004"/>
    <dbReference type="SMR" id="Q59004"/>
    <dbReference type="STRING" id="243232.MJ_1609"/>
    <dbReference type="EnsemblBacteria" id="AAB99636">
        <property type="protein sequence ID" value="AAB99636"/>
        <property type="gene ID" value="MJ_1609"/>
    </dbReference>
    <dbReference type="GeneID" id="1452518"/>
    <dbReference type="KEGG" id="mja:MJ_1609"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q59004"/>
    <dbReference type="OMA" id="REKETHR"/>
    <dbReference type="OrthoDB" id="44502at2157"/>
    <dbReference type="PhylomeDB" id="Q59004"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184676" description="Uncharacterized ATP-binding protein MJ1609">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="374" mass="43985" checksum="211D5BEB98269E9C" modified="1997-11-01" version="1">MKFFNREKEINKILSIIEGEPNNIYFIYGSLNSGKSTLMREIVVNRLDISKYIPFFIDFRTRNILNVDNFIECLFEVDEKSEIDDFREYAKSLADLLVKGSEEISKYYLGMPIKIPKPFFDKIFNKKDKSADVYQYIEYLFAKLNEKGKKPILIFDELQMIKEITLNGNRKLLWSLFQFLVALTKVQHLCHVFCLSSDSLFIEYVYKTGELEGRADYILVDDFDKQTALKFIDFLAKEILNKKLPKDEKELIYSYVGGKPVLIIKVIDKLRYENLNDILDFMLKDATQKLKYFLNDLDYIKPKVEVGDEVIELKKEDIVKALKLFKDSYEISDSEVAKPIYVYLVKKNILFLNPIEGILKPQSFLIWNAIKRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q59024</accession>
    <name>DP2L_METJA</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase II large subunit</fullName>
            <shortName>Pol II</shortName>
            <ecNumber evidence="1">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Exodeoxyribonuclease large subunit</fullName>
            <ecNumber evidence="1">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">polC</name>
        <name type="ordered locus">MJ1630</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="J. Bacteriol." volume="180" first="2232" last="2236">
            <title>A novel DNA polymerase family found in Archaea.</title>
            <authorList>
                <person name="Ishino Y."/>
                <person name="Komori K."/>
                <person name="Cann I.K.O."/>
                <person name="Koga Y."/>
            </authorList>
            <dbReference type="PubMed" id="9555910"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="function">
        <text>Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text>Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="1"/>
    <dbReference type="EC" id="3.1.11.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64503">
        <property type="entry name" value="D64503"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59024"/>
    <dbReference type="SMR" id="Q59024"/>
    <dbReference type="STRING" id="243232.MJ_1630"/>
    <dbReference type="PRIDE" id="Q59024"/>
    <dbReference type="EnsemblBacteria" id="AAB99651">
        <property type="protein sequence ID" value="AAB99651"/>
        <property type="gene ID" value="MJ_1630"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1630"/>
    <dbReference type="eggNOG" id="arCOG04447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001154_0_0_2"/>
    <dbReference type="InParanoid" id="Q59024"/>
    <dbReference type="OMA" id="KRRNCDG"/>
    <dbReference type="PhylomeDB" id="Q59024"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008852">
        <property type="term" value="F:exodeoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000738">
        <property type="term" value="P:DNA catabolic process, exonucleolytic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00324">
        <property type="entry name" value="DNApol_II_L_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="PTHR42210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016275">
        <property type="entry name" value="PolC_DP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000152575" description="DNA polymerase II large subunit">
        <location>
            <begin position="1"/>
            <end position="1139"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="1139" mass="129402" checksum="3B7968A013AAC45A" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q59029</accession>
    <name>Y1635_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TnpB-like protein MJ1635</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1635</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the transposase 2 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the transposase 35 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64504">
        <property type="entry name" value="A64504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871159.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59029"/>
    <dbReference type="STRING" id="243232.MJ_1635"/>
    <dbReference type="EnsemblBacteria" id="AAB99664">
        <property type="protein sequence ID" value="AAB99664"/>
        <property type="gene ID" value="MJ_1635"/>
    </dbReference>
    <dbReference type="GeneID" id="1452544"/>
    <dbReference type="KEGG" id="mja:MJ_1635"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_3_4_2"/>
    <dbReference type="InParanoid" id="Q59029"/>
    <dbReference type="OMA" id="ELYHIHR"/>
    <dbReference type="OrthoDB" id="75656at2157"/>
    <dbReference type="PhylomeDB" id="Q59029"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032196">
        <property type="term" value="P:transposition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001959">
        <property type="entry name" value="Transposase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010095">
        <property type="entry name" value="Transposase_IS605_OrfB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01385">
        <property type="entry name" value="OrfB_IS605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07282">
        <property type="entry name" value="OrfB_Zn_ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01766">
        <property type="entry name" value="tspaseT_teng_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0814">Transposable element</keyword>
    <keyword id="KW-0815">Transposition</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000107451" description="TnpB-like protein MJ1635">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q7DF80"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="414" mass="49003" checksum="031FC33EC1D4F277" modified="1997-11-01" version="1">MSNKTKLKDNKELLYQVVLSYKVSHNYPLKAFLIECKNKLNECIDMIWNNIKYTKKDNPKLPKSNEFKRELRNKLLENWNYASHYIDGIIKTSYSILQSWASNYKRGYRTKTKPIAKRLFVRVKTTLIKYDKEKGEIRITIKPRKDYLILNIKNEWFFDKVKNLTIGEIILKEKETFLTFKDNLNYSDKGMIVGVDSNLRSLDLFHPIEGWTRVDLTELHRIKEVYDRKIDFLKKLLKKFPLRAMRKINRLFERRRNRVKDFLHKLTIQLSRLFPDAIFVFEDLNKRRMYKSKYFNRKIDRVNWNGLIEKISYKTIVILVNPAYTSTICPICGSRMESQEGQVVYCSNCLNSFNRQLVGCYNIFKRGLGNIKEIMGGSGVTTTGVEVSFGKLMTPNPNVIYIVDYNGKYFNEIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q59043</accession>
    <name>Y1649_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ribosomal RNA large subunit methyltransferase MJ1649</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1649</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RlmI family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64505">
        <property type="entry name" value="G64505"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59043"/>
    <dbReference type="SMR" id="Q59043"/>
    <dbReference type="STRING" id="243232.MJ_1649"/>
    <dbReference type="EnsemblBacteria" id="AAB99670">
        <property type="protein sequence ID" value="AAB99670"/>
        <property type="gene ID" value="MJ_1649"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1649"/>
    <dbReference type="eggNOG" id="arCOG00032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014042_0_0_2"/>
    <dbReference type="InParanoid" id="Q59043"/>
    <dbReference type="OMA" id="VMDVFDY"/>
    <dbReference type="PhylomeDB" id="Q59043"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000213172" description="Putative ribosomal RNA large subunit methyltransferase MJ1649">
        <location>
            <begin position="1"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="5"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="387" mass="45222" checksum="7A03FF3C1B6730B6" modified="1996-11-01" version="1">MVMILIKLEIDRRAYNSIKNFSRLVYTKAIKNRGDLPKKEEIVTLTYNGKFVAKALYNPKSVILKILTTEDEEIDYDFFYKRIFNAKIYRENILNYKNTYRWIYAEGDELPTIIFDKYNELGAMQLMSKLIEKEYLKDIVDILFELSDLETIYVKRGKKGERIRDKIFGDKNKFETVIKEGDAKFKVNVRGHKTGFFLDQRENRLYLEKFIKEGDRVLDICCYTGGFSVHAAIRGAEVVGVDLSKKALKLAEENIELNNIPKDRYEFIEGNAFEVMKEMIEDKEKFDVVILDPPAFTQTEDDIKNALRAYASLNYLGIKLAKRIFVTCSCSHHVDKEMFKRTVISSAFRAKKELIMIDYKGQAPDHPISIGNKNLEYLKCIFFYVKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q59045</accession>
    <name>SALL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Chlorinase MJ1651</fullName>
            <ecNumber>2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1651</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Proteins" volume="70" first="572" last="577">
            <title>Crystal structure of a conserved protein of unknown function (MJ1651) from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Rao K.N."/>
                <person name="Burley S.K."/>
                <person name="Swaminathan S."/>
            </authorList>
            <dbReference type="PubMed" id="17910070"/>
            <dbReference type="DOI" id="10.1002/prot.21646"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 2-261</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64506">
        <property type="entry name" value="A64506"/>
    </dbReference>
    <dbReference type="PDB" id="2F4N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B/C=2-261"/>
    </dbReference>
    <dbReference type="PDBsum" id="2F4N"/>
    <dbReference type="AlphaFoldDB" id="Q59045"/>
    <dbReference type="SMR" id="Q59045"/>
    <dbReference type="STRING" id="243232.MJ_1651"/>
    <dbReference type="PRIDE" id="Q59045"/>
    <dbReference type="EnsemblBacteria" id="AAB99672">
        <property type="protein sequence ID" value="AAB99672"/>
        <property type="gene ID" value="MJ_1651"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1651"/>
    <dbReference type="eggNOG" id="arCOG04309">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059734_1_1_2"/>
    <dbReference type="InParanoid" id="Q59045"/>
    <dbReference type="OMA" id="EGYVGAM"/>
    <dbReference type="PhylomeDB" id="Q59045"/>
    <dbReference type="EvolutionaryTrace" id="Q59045"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_Chlor/Fluor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="PTHR35092"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_adeno_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006779">
        <property type="entry name" value="UCP006779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="SSF101852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="SSF102522"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107459" description="Chlorinase MJ1651">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="80"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="135"/>
            <end position="138"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>chloride</name>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="12"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="21"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="45"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="69"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="76"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="91"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="101"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="111"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="119"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="172"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="178"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="182"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="188"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="199"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="206"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="210"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="219"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="223"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="229"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="238"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="247"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="258"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17910070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2F4N"/>
        </source>
    </evidence>
    <sequence length="263" mass="30249" checksum="801E2C3E5A66CDD0" modified="1996-11-01" version="1">MGIYMRDDILDIITLTTDFGTNEGYVGAMKGRILNILKKYNKDAKIIDISHEIKPFNIYHGAYVLLTAIPYFPPSVHVAVIDPTVGSERKSIVIETKSGYYLVGPDNGLFTYVAEKLGIKRIIKIDEERYKPSSTFHGRDVYAVVGAEILINNGYDGEELDEMVKIDETKKRVIHIDRFGNIITNIKKDEVTFKYYDTIMIKIRHKNGIEKIIKCKFVKSYFEEKNNFICLINSEGFLEISKFMDNASKLLNVDYLDEIEIIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q59072</accession>
    <name>SPSS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>O-phospho-L-seryl-tRNA:Cys-tRNA synthase</fullName>
            <ecNumber>2.5.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Sep-tRNA:Cys-tRNA synthase</fullName>
            <shortName>SepCysS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pscS</name>
        <name type="ordered locus">MJ1678</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Science" volume="307" first="1969" last="1972">
            <title>RNA-dependent cysteine biosynthesis in archaea.</title>
            <authorList>
                <person name="Sauerwald A."/>
                <person name="Zhu W."/>
                <person name="Major T.A."/>
                <person name="Roy H."/>
                <person name="Palioura S."/>
                <person name="Jahn D."/>
                <person name="Whitman W.B."/>
                <person name="Yates J.R. III"/>
                <person name="Ibba M."/>
                <person name="Soell D."/>
            </authorList>
            <dbReference type="PubMed" id="15790858"/>
            <dbReference type="DOI" id="10.1126/science.1108329"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="Nat. Struct. Mol. Biol." volume="15" first="507" last="514">
            <title>Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys).</title>
            <authorList>
                <person name="Zhang C.-M."/>
                <person name="Liu C."/>
                <person name="Slater S."/>
                <person name="Hou Y.-M."/>
            </authorList>
            <dbReference type="PubMed" id="18425141"/>
            <dbReference type="DOI" id="10.1038/nsmb.1423"/>
        </citation>
        <scope>INTERACTION WITH SEPRS</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + hydrogen sulfide + O-phospho-L-seryl-tRNA(Cys) = L-cysteinyl-tRNA(Cys) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25686"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="EC" id="2.5.1.73"/>
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    <sequence length="377" mass="42764" checksum="791842FF733C72BA" modified="2009-01-20" version="2">MDKYKNLTRSLTREFINLNPIQRGGILPKEAKKAVYEYWDGYSVCDYCHGRLDEVTCPPIKDFLEDIAKFLNMDCARPTHGAREGKFIVMHAICKEGDYVVLDKNAHYTSYVAAERAKLNVAEVGYEEEYPTYKINLEGYKEVIDNLEDKGKNVGLILLTHVDGEYGNLNDAKKVGKIAKEKGIPFLLNCAYTVGRMPVNGKEVKADFIVASGHKSMAASAPCGILAFSEEFSDKITKTSEKFPVKEIEMLGCTSRGLPIVTLMASFPHVVERVKKWDEELKKTRYVVDELEKIGFKQLGIKPKEHDLIKFETPVLDEIAKKDKRRGFFFYDELKKRGIGGIRAGVTKEIKMSVYGLEWEQVEYVVNAIKEIVESCK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q60260</accession>
    <name>Y3504_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJECL04</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL04</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
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        <name>large ECE</name>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
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            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
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    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="439" mass="52422" checksum="AA50918A58EFC354" modified="1997-11-01" version="1">MFVDREEELKALNEKLDSNNFEFIVIYGRRRIGKTKLALKSVENREHIYYLAVEGDNLKHFKRYASKVEPTIEYAKEDWEAYFNFLKDKIIIIDEFPNLIKENPNVLSLFQRIVDIHLKNTKTKLIILGSSISMMGEKVLSYKSPLYGRKTGVLKIKPLKFKHLKEFFPKAIWEELVEIYGFADGIPYYLEKVKLPFWDYLDKEIKRVDSFLRYEVDFLMKYEFEEPTTYKKILEAIAFGNHTLGEIKNYLGFKHSDLTPYLKNLIEVEFIERQTPITESVKSKKGRYYIKDNFIAFYFRYIFPNLSAIEEGIFDIEEIKADYNQYLGFVFEKVAKEFLIELNKMNKLPFKFLKIGRWWHRGEEIDLIALNDNDKKALFVEVKWKDLKDRDVKKIYRDLYRKSKLVGLDDYEKYYAIVGKKIESKENGDCLLFDLEDFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q60301</accession>
    <name>T2RM_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative type II restriction enzyme and methyltransferase RM.MjaORFECS2P</fullName>
            <shortName evidence="1">RM.MjaORFECS2P</shortName>
            <ecNumber>3.1.21.4</ecNumber>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName>Adenine-specific methyltransferase activity</fullName>
                <ecNumber>2.1.1.72</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name type="ordered locus">MJECS02</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPES</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Probably a G subtype restriction enzyme that recognizes an undetermined sequence and cleaves at an undetermined site. Probably also acts as an alpha subtype methylase, presumably on the same sequence.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.21.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.4"/>
    <dbReference type="EC" id="2.1.1.72"/>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64516">
        <property type="entry name" value="B64516"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890095.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60301"/>
    <dbReference type="STRING" id="243232.MJ_ECS02"/>
    <dbReference type="REBASE" id="6813">
        <property type="entry name" value="MjaORFECS2P"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q60301"/>
    <dbReference type="DNASU" id="1450829"/>
    <dbReference type="EnsemblBacteria" id="AAC37060">
        <property type="protein sequence ID" value="AAC37060"/>
        <property type="gene ID" value="MJ_ECS02"/>
    </dbReference>
    <dbReference type="GeneID" id="1450829"/>
    <dbReference type="KEGG" id="mja:MJ_ECS02"/>
    <dbReference type="eggNOG" id="arCOG02635">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_270917_0_0_2"/>
    <dbReference type="InParanoid" id="Q60301"/>
    <dbReference type="OMA" id="HEENIGD"/>
    <dbReference type="OrthoDB" id="1425at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011639">
        <property type="entry name" value="RM_methylase_Eco57I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025931">
        <property type="entry name" value="TaqI_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07669">
        <property type="entry name" value="Eco57I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12950">
        <property type="entry name" value="TaqI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000088002" description="Putative type II restriction enzyme and methyltransferase RM.MjaORFECS2P">
        <location>
            <begin position="1"/>
            <end position="1181"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="1181" mass="138439" checksum="9F961D8F8C6A4BDD" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q60332</accession>
    <name>Y021_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative glycyl-radical enzyme activating enzyme MJ0021</fullName>
            <shortName evidence="3">GRE activating enzyme MJ0021</shortName>
            <ecNumber evidence="1">1.97.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0021</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:61976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:14480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15993"/>
            <dbReference type="Rhea" id="RHEA-COMP:15994"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29947"/>
            <dbReference type="ChEBI" id="CHEBI:32722"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:140311"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the organic radical-activating enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.97.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64302">
        <property type="entry name" value="E64302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869513.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60332"/>
    <dbReference type="STRING" id="243232.MJ_0021"/>
    <dbReference type="EnsemblBacteria" id="AAB98003">
        <property type="protein sequence ID" value="AAB98003"/>
        <property type="gene ID" value="MJ_0021"/>
    </dbReference>
    <dbReference type="GeneID" id="1450859"/>
    <dbReference type="KEGG" id="mja:MJ_0021"/>
    <dbReference type="eggNOG" id="arCOG00932">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053467_0_0_2"/>
    <dbReference type="InParanoid" id="Q60332"/>
    <dbReference type="OMA" id="DCFYCPL"/>
    <dbReference type="OrthoDB" id="85003at2157"/>
    <dbReference type="PhylomeDB" id="Q60332"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040087">
        <property type="entry name" value="MJ0021-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001989">
        <property type="entry name" value="Radical_activat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="PTHR43288:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01108">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01087">
        <property type="entry name" value="RADICAL_ACTIVATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000200544" description="Putative glycyl-radical enzyme activating enzyme MJ0021">
        <location>
            <begin position="1"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="23"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0A9N4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="375" mass="43446" checksum="5AEA168D21383CC8" modified="1997-11-01" version="1">MNVEEIEKYLEENFDKLPEGCKQCVKGGKLVLFITGICNNNCYYCPLSEKRKNKDVIYANERLITTVEEAIEEAKLCSSKGVGITGGNPLLKINRTVKFLKALKKEFDEFHAHLYTTPETVNEENLKLLKEADLDEIRLHPTKIFNEGYDEEYIKFLCNKLNLCNKYIEDVGVEIPAIPNMENEILKLAEAIDGIAKFMNINELEFSEENYHELEKRGFMPKDDVSNAIAGSEETALKVIKEFKGDLFINYCPSVLKDAIQMRNRLINRAKNVAKPYEVITEDGLLLRGIMIFDNEDDLKEMAEILEENEIEFEIIDKNICLNPFILEDIIEEMKRQRFPITFSAYISELYPTADALEVERIPLITKKLKFRRRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q60333</accession>
    <name>Y023_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0023</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0023</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the M.jannaschii MJ0023/MJ0349/MJ1072/MJ1074/MJ1107/MJECL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64302">
        <property type="entry name" value="G64302"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60333"/>
    <dbReference type="SMR" id="Q60333"/>
    <dbReference type="EnsemblBacteria" id="AAB98012">
        <property type="protein sequence ID" value="AAB98012"/>
        <property type="gene ID" value="MJ_0023"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0023"/>
    <dbReference type="eggNOG" id="arCOG09652">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165881_0_0_2"/>
    <dbReference type="OMA" id="AMYAPEI"/>
    <dbReference type="PhylomeDB" id="Q60333"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106659" description="Uncharacterized protein MJ0023">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="118" mass="13938" checksum="E00CAF8EA0E72809" modified="1997-11-01" version="1">MKSIRISSDYRAKRDNASCFDETFLKSFAEELYNAIIEIIKENKTIIKNEVRDELRNELATKEDILLVEERLGKKIELLNQKIEREIKLVRRDMIIINLVIILAMYAPEIIGKLLIFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q60362</accession>
    <name>NAH1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Na(+)/H(+) antiporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>MjNhaP1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0057</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="FEBS Lett." volume="527" first="245" last="249">
            <title>Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii.</title>
            <authorList>
                <person name="Hellmer J."/>
                <person name="Paetzold R."/>
                <person name="Zeilinger C."/>
            </authorList>
            <dbReference type="PubMed" id="12220668"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(02)03244-1"/>
        </citation>
        <scope>FUNCTION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="FEBS Lett." volume="542" first="32" last="36">
            <title>Conserved arginine and aspartate residues are critical for function of MjNhaP1, a Na+/H+ antiporter of M. jannaschii.</title>
            <authorList>
                <person name="Hellmer J."/>
                <person name="Teubner A."/>
                <person name="Zeilinger C."/>
            </authorList>
            <dbReference type="PubMed" id="12729893"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(03)00332-6"/>
        </citation>
        <scope>MUTAGENESIS OF ASP-93; ASP-132; GLU-156; ASP-161; HIS-211; HIS-215; ARG-320; HIS-333 AND ARG-347</scope>
    </reference>
    <comment type="function">
        <text evidence="2">This is a Na(+)/H(+) antiporter. Can also transport lithium.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Active at pH 7.0 and below.</text>
        </phDependence>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-8539024">
            <id>Q60362</id>
        </interactant>
        <interactant intactId="EBI-8539024">
            <id>Q60362</id>
            <label>MJ0057</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64307">
        <property type="entry name" value="A64307"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869549.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4CZB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="A/B/C/D=1-426"/>
    </dbReference>
    <dbReference type="PDB" id="4D0A">
        <property type="method" value="EM"/>
        <property type="resolution" value="6.00 A"/>
        <property type="chains" value="B=1-426"/>
    </dbReference>
    <dbReference type="PDBsum" id="4CZB"/>
    <dbReference type="PDBsum" id="4D0A"/>
    <dbReference type="AlphaFoldDB" id="Q60362"/>
    <dbReference type="SMR" id="Q60362"/>
    <dbReference type="MINT" id="Q60362"/>
    <dbReference type="STRING" id="243232.MJ_0057"/>
    <dbReference type="TCDB" id="2.A.36.6.6">
        <property type="family name" value="the monovalent cation:proton antiporter-1 (cpa1) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98037">
        <property type="protein sequence ID" value="AAB98037"/>
        <property type="gene ID" value="MJ_0057"/>
    </dbReference>
    <dbReference type="GeneID" id="1450896"/>
    <dbReference type="KEGG" id="mja:MJ_0057"/>
    <dbReference type="eggNOG" id="arCOG01961">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_9_3_2"/>
    <dbReference type="InParanoid" id="Q60362"/>
    <dbReference type="OMA" id="KWEGILI"/>
    <dbReference type="OrthoDB" id="47913at2157"/>
    <dbReference type="PhylomeDB" id="Q60362"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0050">Antiport</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0739">Sodium transport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000052402" description="Na(+)/H(+) antiporter 1">
        <location>
            <begin position="1"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="208"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in activity." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Almost no change in activity." evidence="3">
        <original>E</original>
        <variation>A</variation>
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="3">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No change in activity; when associated with R-215." evidence="3">
        <original>H</original>
        <variation>R</variation>
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No change in activity; when associated with R-211." evidence="3">
        <original>H</original>
        <variation>R</variation>
        <location>
            <position position="215"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <variation>D</variation>
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Almost no change in activity." evidence="3">
        <original>R</original>
        <variation>H</variation>
        <location>
            <position position="320"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No change in activity." evidence="3">
        <original>H</original>
        <variation>R</variation>
        <location>
            <position position="333"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in activity." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="347"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="2"/>
            <end position="26"/>
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        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="43"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="51"/>
            <end position="76"/>
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    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="82"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="86"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="95"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="133"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="141"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="177"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="183"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="215"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="218"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="236"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="248"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="269"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="319"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="335"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="349"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="368"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="372"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="379"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12220668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12729893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4CZB"/>
        </source>
    </evidence>
    <sequence length="426" mass="45966" checksum="967F9A3E60949A1D" modified="1997-11-01" version="1">MELMMAIGYLGLALVLGSLVAKIAEKLKIPDIPLLLLLGLIIGPFLQIIPSDSAMEIFEYAGPIGLIFILLGGAFTMRISLLKRVIKTVVRLDTITFLITLLISGFIFNMVLNLPYTSPVGYLFGAITAATDPATLIPVFSRVRTNPEVAITLEAESIFNDPLGIVSTSVILGLFGLFSSSNPLIDLITLAGGAIVVGLLLAKIYEKIIIHCDFHEYVAPLVLGGAMLLLYVGDDLLPSICGYGFSGYMAVAIMGLYLGDALFRADDIDYKYIVSFCDDLSLLARVFIFVFLGACIKLSMLENYFIPGLLVALGSIFLARPLGVFLGLIGSKHSFKEKLYFALEGPRGVVPAALAVTVGIEILKNADKIPASITKYITPTDIAGTIIIGTFMTILLSVILEASWAGMLALKLLGEYKPKYKEESHH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>O06918</accession>
    <name>Y353_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0353</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0353</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64344">
        <property type="entry name" value="A64344"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O06918"/>
    <dbReference type="SMR" id="O06918"/>
    <dbReference type="STRING" id="243232.MJ_0353"/>
    <dbReference type="PRIDE" id="O06918"/>
    <dbReference type="EnsemblBacteria" id="AAB98342">
        <property type="protein sequence ID" value="AAB98342"/>
        <property type="gene ID" value="MJ_0353"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0353"/>
    <dbReference type="eggNOG" id="arCOG08302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_190396_0_0_2"/>
    <dbReference type="OMA" id="SYGCAKS"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106824" description="Uncharacterized protein MJ0353">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <sequence length="84" mass="9983" checksum="6ABE05448DB8A210" modified="1997-07-01" version="1">MMRYSSNKIYFIVYLGGKPVSFGVAKDVDEFERRRENIECLSDKIRVVKVGKKLFKRLRRQILEGEKKDFGMLKVNRRDLNVQV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>P54023</accession>
    <name>RL13_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl13</name>
        <name type="ordered locus">MJ0194</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869689.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54023"/>
    <dbReference type="SMR" id="P54023"/>
    <dbReference type="STRING" id="243232.MJ_0194"/>
    <dbReference type="EnsemblBacteria" id="AAB98174">
        <property type="protein sequence ID" value="AAB98174"/>
        <property type="gene ID" value="MJ_0194"/>
    </dbReference>
    <dbReference type="GeneID" id="1451042"/>
    <dbReference type="KEGG" id="mja:MJ_0194"/>
    <dbReference type="eggNOG" id="arCOG04242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076922_1_0_2"/>
    <dbReference type="InParanoid" id="P54023"/>
    <dbReference type="OMA" id="GMLPWKT"/>
    <dbReference type="OrthoDB" id="87367at2157"/>
    <dbReference type="PhylomeDB" id="P54023"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_L13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_L13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_L13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_L13_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="PTHR11545"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="PTHR11545:SF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="SSF52161"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000133760" description="50S ribosomal protein L13">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <sequence length="137" mass="15786" checksum="F92D686B0D9D0E29" modified="1998-07-15" version="2">MTVIDAEGAILGRLASEVAKRVLRGEEIVIVNAEMVVITGNKDWIIKTYQEEREKKNVANPRRFGPKFPRRPDDILRRTIRKMLPYKKPKGREAFKRVKVYVGNPKNLTVDEKISHKLNTTKYITLAELSKHLGAKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>P54037</accession>
    <name>RL14_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl14</name>
        <name type="ordered locus">MJ0466</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64358">
        <property type="entry name" value="B64358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869966.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54037"/>
    <dbReference type="SMR" id="P54037"/>
    <dbReference type="STRING" id="243232.MJ_0466"/>
    <dbReference type="EnsemblBacteria" id="AAB98455">
        <property type="protein sequence ID" value="AAB98455"/>
        <property type="gene ID" value="MJ_0466"/>
    </dbReference>
    <dbReference type="GeneID" id="1451328"/>
    <dbReference type="GeneID" id="65883006"/>
    <dbReference type="KEGG" id="mja:MJ_0466"/>
    <dbReference type="eggNOG" id="arCOG04095">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095071_3_0_2"/>
    <dbReference type="InParanoid" id="P54037"/>
    <dbReference type="OMA" id="AKEVLCI"/>
    <dbReference type="OrthoDB" id="91632at2157"/>
    <dbReference type="PhylomeDB" id="P54037"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_L14_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_L14P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_L14P_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_L14P_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="PTHR11761"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="SSF50193"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000128573" description="50S ribosomal protein L14">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <sequence length="132" mass="14271" checksum="C02B0EE98C742F3D" modified="1996-10-01" version="1">MKAIGSKPVRALPVGARCICADNTGAKEVEIIAVRNYKGVARRLPTARVGDMVIVTVKKGTPEMRKQVLPAVVIRQRKEIRRPDGTRVKFADNAVVIVTPDGNPKGSDIKGPVAKEAAERWPGIARIAKIII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P54044</accession>
    <name>RL18_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18</name>
        <name type="ordered locus">MJ0474</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This is one of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64359">
        <property type="entry name" value="B64359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869975.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54044"/>
    <dbReference type="SMR" id="P54044"/>
    <dbReference type="STRING" id="243232.MJ_0474"/>
    <dbReference type="EnsemblBacteria" id="AAB98463">
        <property type="protein sequence ID" value="AAB98463"/>
        <property type="gene ID" value="MJ_0474"/>
    </dbReference>
    <dbReference type="GeneID" id="1451336"/>
    <dbReference type="KEGG" id="mja:MJ_0474"/>
    <dbReference type="eggNOG" id="arCOG04088">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_2_0_2"/>
    <dbReference type="InParanoid" id="P54044"/>
    <dbReference type="OMA" id="MAHGPRY"/>
    <dbReference type="OrthoDB" id="108717at2157"/>
    <dbReference type="PhylomeDB" id="P54044"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_L18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_L5_euk/L18_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="PTHR23410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131405" description="50S ribosomal protein L18">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <sequence length="195" mass="22354" checksum="E2637368BAADECDF" modified="1996-10-01" version="1">MATGPTYRVKFRRRREAKTDYRKRLKLLLSRKPRLVARRTLNHCIAQIVLYDEKGDKTVVSAHSRELIKLGYKGHTGNLPSAYLTGYLLGKKALAKGYTEAVLDIGLHRATKGNAIFAILKGALDAGMEIPHGEEILPSEERIRGEHIKAYAEMLKEQDEERYKKQFSKYLEKGLEPEKLPEHFEEIKAKIDSMF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>P54045</accession>
    <name>RS5_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps5</name>
        <name type="ordered locus">MJ0475</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text>The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64359">
        <property type="entry name" value="C64359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869976.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54045"/>
    <dbReference type="SMR" id="P54045"/>
    <dbReference type="STRING" id="243232.MJ_0475"/>
    <dbReference type="EnsemblBacteria" id="AAB98464">
        <property type="protein sequence ID" value="AAB98464"/>
        <property type="gene ID" value="MJ_0475"/>
    </dbReference>
    <dbReference type="GeneID" id="1451337"/>
    <dbReference type="KEGG" id="mja:MJ_0475"/>
    <dbReference type="eggNOG" id="arCOG04087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065898_0_1_2"/>
    <dbReference type="InParanoid" id="P54045"/>
    <dbReference type="OMA" id="KRGCGSW"/>
    <dbReference type="OrthoDB" id="60954at2157"/>
    <dbReference type="PhylomeDB" id="P54045"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_S5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_S5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_S5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_S5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_S5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_S5_N_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="PTHR13718"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131648" description="30S ribosomal protein S5">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="S5 DRBM" evidence="1">
        <location>
            <begin position="49"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <sequence length="217" mass="23839" checksum="4C84EB0E6D336A08" modified="1996-10-01" version="1">MRFNIDEWEPKTTIGRMVKEGQITDIDYILDNNLPILEPEIVDALLPDLEEKVLDVKLVQRMHKSGRRARFRATVVVGNRNGYVGVGKGKAKEVGPAIRKAIAQAKKNIIRVKRGCGSWECGCGTPHSIPYKGYGKCGSTAIEILPAPKGVGLVAGDVAKAVLGLAGIKDVWTKTFGETRTTYNFAMATFEALKSLNFTRTMEKHKEKLGIVEGRVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>P81319</accession>
    <name>YC8B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1282.2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1282.2</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81319"/>
    <dbReference type="STRING" id="243232.MJ_1282.2"/>
    <dbReference type="EnsemblBacteria" id="AAB99299">
        <property type="protein sequence ID" value="AAB99299"/>
        <property type="gene ID" value="MJ_1282.2"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1282.2"/>
    <dbReference type="eggNOG" id="arCOG08268">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1500293_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107251" description="Uncharacterized protein MJ1282.2">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <sequence length="179" mass="20501" checksum="BB9FCE006025603D" modified="1998-07-15" version="1">MAKDVFNNSLAENVSFINTSGYYNNSENVTYINMSMNGSFSGILYVKSSYKNYTITINESGNFVFNDTTSPIEVELLNNYSDVILNYNLNESINNFSDTSYLILNESCKNSYFNVIYGNSPMLYVSLHDEDFNHNITIFNPQKGISSKGFVLTDIFITTPRMFYSSLNNSFEYQSWNIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>P81327</accession>
    <name>YD3A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1333.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1333.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870851.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81327"/>
    <dbReference type="SMR" id="P81327"/>
    <dbReference type="STRING" id="243232.MJ_1333.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99347">
        <property type="protein sequence ID" value="AAB99347"/>
        <property type="gene ID" value="MJ_1333.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1452236"/>
    <dbReference type="KEGG" id="mja:MJ_1333.1"/>
    <dbReference type="eggNOG" id="arCOG03426">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870747_0_0_2"/>
    <dbReference type="OMA" id="YKGKASI"/>
    <dbReference type="OrthoDB" id="122771at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107279" description="Uncharacterized protein MJ1333.1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="EamA">
        <location>
            <begin position="13"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="137" mass="14847" checksum="61C99BEDD5157636" modified="1998-06-01" version="1">MDTAIILGLLVAVFYGVGTFFAKIVCEKNPLFQWIVVNIVGIILCLIILLKYKNIIITDQKILTYAIISAVLVVIGSLLLYYALYKGKASIVVPLSSIGPAITVALSILFLKETLTLPQMIGIVLIIIGIILLSISN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q57558</accession>
    <name>Y093_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0093</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0093</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64311">
        <property type="entry name" value="E64311"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869585.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57558"/>
    <dbReference type="SMR" id="Q57558"/>
    <dbReference type="STRING" id="243232.MJ_0093"/>
    <dbReference type="EnsemblBacteria" id="AAB98076">
        <property type="protein sequence ID" value="AAB98076"/>
        <property type="gene ID" value="MJ_0093"/>
    </dbReference>
    <dbReference type="GeneID" id="1450932"/>
    <dbReference type="KEGG" id="mja:MJ_0093"/>
    <dbReference type="eggNOG" id="arCOG05020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850644_0_0_2"/>
    <dbReference type="OMA" id="DYIFLFT"/>
    <dbReference type="OrthoDB" id="96760at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106689" description="Uncharacterized protein MJ0093">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <sequence length="142" mass="16643" checksum="B268466A1D45CEDD" modified="1997-11-01" version="1">MENFNFKCYDIDEKEIPIPPGLPQSIIARLIEICNVKFDIREDEIYNVKYPVLIGKEEDLKEAKKYLELITEAKLTLRDIARLARRFKVKAKIYTDDEDLRYILDVLSNDIANKDYIEIVEEMPEGDKEVIEIGDKKIYVGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57560</accession>
    <name>Y095_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0095</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0095</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64311">
        <property type="entry name" value="G64311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57560"/>
    <dbReference type="STRING" id="243232.MJ_0095"/>
    <dbReference type="EnsemblBacteria" id="AAB98086">
        <property type="protein sequence ID" value="AAB98086"/>
        <property type="gene ID" value="MJ_0095"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0095"/>
    <dbReference type="eggNOG" id="arCOG05010">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143941_0_0_2"/>
    <dbReference type="InParanoid" id="Q57560"/>
    <dbReference type="OMA" id="INIAHHV"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014514">
        <property type="entry name" value="UCP021940"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09974">
        <property type="entry name" value="DUF2209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021940">
        <property type="entry name" value="UCP021940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106691" description="Uncharacterized protein MJ0095">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <sequence length="126" mass="14709" checksum="D41D24ABDD043E9B" modified="1997-11-01" version="1">MAIDISGRHHENDIFFRVYAGVLVEIKADRIVHVEKIDVMVKEEETQKLRDIVKEVKELIDKVGDEFDYILCERGEFFNISKDIISAILKKEVIFPKTRGELEAINIAHHVSYSVRKLLIEEKRKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q57587</accession>
    <name>Y123_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0123</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0123</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1213 and A.aeolicus AA15.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64315">
        <property type="entry name" value="C64315"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869616.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4JIX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=1-110"/>
    </dbReference>
    <dbReference type="PDBsum" id="4JIX"/>
    <dbReference type="AlphaFoldDB" id="Q57587"/>
    <dbReference type="SMR" id="Q57587"/>
    <dbReference type="STRING" id="243232.MJ_0123"/>
    <dbReference type="MEROPS" id="M95.002"/>
    <dbReference type="EnsemblBacteria" id="AAB98111">
        <property type="protein sequence ID" value="AAB98111"/>
        <property type="gene ID" value="MJ_0123"/>
    </dbReference>
    <dbReference type="GeneID" id="1450966"/>
    <dbReference type="KEGG" id="mja:MJ_0123"/>
    <dbReference type="eggNOG" id="arCOG02625">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2340025_0_0_2"/>
    <dbReference type="OMA" id="WREVEKV"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002725">
        <property type="entry name" value="YgjP-like_metallopeptidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01863">
        <property type="entry name" value="DUF45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106703" description="Uncharacterized protein MJ0123">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="28"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="38"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="52"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="60"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="83"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="95"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="4JIX"/>
        </source>
    </evidence>
    <sequence length="110" mass="13187" checksum="B905F6BBFBF207DF" modified="1997-11-01" version="1">MKINENKKDIKDIVNEILISLNINESINIEIKPMKQKIASFSFKTKTLRLNKYVVENFDEELLHYIILHELIHFKIKSINHGIKFENELRNYFSKNECDEIELKIIQKLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57606</accession>
    <name>Y141_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ0141</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ0141</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0141</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Putative antitoxin component of a putative type VII toxin-antitoxin (TA) system. Its cognate toxin might be MF0142, which it might AMPylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64317">
        <property type="entry name" value="F64317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869636.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57606"/>
    <dbReference type="SMR" id="Q57606"/>
    <dbReference type="STRING" id="243232.MJ_0141"/>
    <dbReference type="EnsemblBacteria" id="AAB98124">
        <property type="protein sequence ID" value="AAB98124"/>
        <property type="gene ID" value="MJ_0141"/>
    </dbReference>
    <dbReference type="GeneID" id="1450985"/>
    <dbReference type="KEGG" id="mja:MJ_0141"/>
    <dbReference type="eggNOG" id="arCOG01201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_3_2_2"/>
    <dbReference type="InParanoid" id="Q57606"/>
    <dbReference type="OMA" id="YPDEEAQ"/>
    <dbReference type="OrthoDB" id="108083at2157"/>
    <dbReference type="PhylomeDB" id="Q57606"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106717" description="Putative protein adenylyltransferase MJ0141">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="29"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="99" mass="11578" checksum="2199CE924EE8FADD" modified="1997-11-01" version="1">MEIIEIIKEFKKDISTILKDKLDRVILFGSYARGDYDEESDVDVLILVKEMPTLKEKQKIIKIASRYSLKYDILISPIIYKKTIKTSFIDEVENYGVEV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q57619</accession>
    <name>FER8_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0155</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0155</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 4 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64319">
        <property type="entry name" value="D64319"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869650.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57619"/>
    <dbReference type="SMR" id="Q57619"/>
    <dbReference type="STRING" id="243232.MJ_0155"/>
    <dbReference type="EnsemblBacteria" id="AAB98137">
        <property type="protein sequence ID" value="AAB98137"/>
        <property type="gene ID" value="MJ_0155"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="7Fe_ferredoxin"/>
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        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="7FE8SFRDOXIN"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159136" description="Uncharacterized ferredoxin MJ0155">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
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        <location>
            <begin position="4"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="93"/>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
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            <position position="16"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        <ligand>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        <location>
            <position position="83"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
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    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="111"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
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    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="151" mass="16985" checksum="86EC9B27DEFAEAF1" modified="1996-11-01" version="1">MNPKIIVLNPEKCTKCYDCINICKEIHGESRVRKVDGIPIFCMQCENAPCKEICPVDAIYLKDGIPIVDKERCIACGMCAIACPIGAIFIKNRVAHKCTLCLDVDRITPACVEACKDKALLLVSEETLDMMKEEKRKKILKILREEAKENL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57646</accession>
    <name>CG121_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Regulatory protein Cgi121</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Positive regulator of Bud32 kinase activity</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cgi121</name>
        <name type="ordered locus">MJ0187</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Mol. Cell" volume="32" first="259" last="275">
            <title>Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.</title>
            <authorList>
                <person name="Mao D.Y."/>
                <person name="Neculai D."/>
                <person name="Downey M."/>
                <person name="Orlicky S."/>
                <person name="Haffani Y.Z."/>
                <person name="Ceccarelli D.F."/>
                <person name="Ho J.S."/>
                <person name="Szilard R.K."/>
                <person name="Zhang W."/>
                <person name="Ho C.S."/>
                <person name="Wan L."/>
                <person name="Fares C."/>
                <person name="Rumpel S."/>
                <person name="Kurinov I."/>
                <person name="Arrowsmith C.H."/>
                <person name="Durocher D."/>
                <person name="Sicheri F."/>
            </authorList>
            <dbReference type="PubMed" id="18951093"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2008.10.002"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.60 ANGSTROMS) IN COMPLEX WITH KAE1-BUD32</scope>
        <scope>STRUCTURE BY NMR</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF LEU-134; ALA-138 AND LEU-139</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, a step in the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Cgi121 stimulates Bud32 kinase activity via an activation of Bud32 autophosphorylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CGI121/TPRKB family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB98167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D64323"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="chains" value="A=1-145"/>
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    <dbReference type="PDB" id="3ENH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.60 A"/>
        <property type="chains" value="C/D=1-145"/>
    </dbReference>
    <dbReference type="PDB" id="7KJT">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.34 A"/>
        <property type="chains" value="H=1-145"/>
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    <dbReference type="PDBsum" id="2K8Y"/>
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    <dbReference type="SMR" id="Q57646"/>
    <dbReference type="STRING" id="243232.MJ_0187"/>
    <dbReference type="EnsemblBacteria" id="AAB98167">
        <property type="protein sequence ID" value="AAB98167"/>
        <property type="gene ID" value="MJ_0187"/>
    </dbReference>
    <dbReference type="GeneID" id="1451034"/>
    <dbReference type="KEGG" id="mja:MJ_0187"/>
    <dbReference type="eggNOG" id="arCOG02197">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801715_0_0_2"/>
    <dbReference type="InParanoid" id="Q57646"/>
    <dbReference type="OMA" id="FWMEILV"/>
    <dbReference type="OrthoDB" id="90121at2157"/>
    <dbReference type="PhylomeDB" id="Q57646"/>
    <dbReference type="EvolutionaryTrace" id="Q57646"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="SSF143870"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106735" description="Regulatory protein Cgi121">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on Bud32 binding." evidence="1">
        <original>L</original>
        <variation>E</variation>
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Severely affects Bud32 binding and does not stimulate Bud32 kinase activity; when associated with R-138." evidence="1">
        <original>L</original>
        <variation>E</variation>
        <location>
            <position position="134"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on Bud32 binding." evidence="1">
        <original>A</original>
        <variation>R</variation>
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Severely affects Bud32 binding and does not stimulate Bud32 kinase activity; when associated with E-134." evidence="1">
        <original>A</original>
        <variation>R</variation>
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on Bud32 binding." evidence="1">
        <original>L</original>
        <variation>R</variation>
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="13"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="21"/>
            <end position="26"/>
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    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="27"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="49"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="53"/>
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    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="67"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="104"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="139"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="18951093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="7KJT"/>
        </source>
    </evidence>
    <sequence length="145" mass="16593" checksum="E7CB7330D1E1003F" modified="1996-11-01" version="1">MIIRGIRGARINNEIFNLGLKFQILNADVVATKKHVLHAINQAKTKKPIAKSFWMEILVRASGQRQIHEAIKIIGAKDGNVCLICEDEETFRKIYELIGGEIDDSVLEINEDKERLIREIFKIRGFGNVVERVLEKIALIELKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="140" xmlns="http://uniprot.org/uniprot">
    <accession>Q57658</accession>
    <name>DHAS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">ASA dehydrogenase</shortName>
            <shortName evidence="1">ASADH</shortName>
            <ecNumber>1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">asd</name>
        <name type="ordered locus">MJ0205</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
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                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
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                <person name="Gocayne J.D."/>
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                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
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                <person name="Smith H.O."/>
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            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Mol. Biol." volume="353" first="1055" last="1068">
            <title>A new branch in the family: structure of aspartate-beta-semialdehyde dehydrogenase from Methanococcus jannaschii.</title>
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                <person name="Faehnle C.R."/>
                <person name="Ohren J.F."/>
                <person name="Viola R.E."/>
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            <dbReference type="PubMed" id="16225889"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2005.09.027"/>
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        <scope>X-RAY CRYSTALLOGRAPHY (2.29 ANGSTROMS) IN COMPLEX WITH NADP</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COENZYME SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>DOMAIN</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate. To a lesser extent, is able to use NAD instead of NADP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-aspartate 4-semialdehyde + NADP(+) + phosphate = 4-phospho-L-aspartate + H(+) + NADPH</text>
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    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">13 uM for NADP(+)</KM>
            <KM evidence="2">1700 uM for NAD(+)</KM>
            <text>kcat is 4 sec(-1) for the L-4-aspartyl-phosphate-forming reaction (at 70 degrees Celsius).</text>
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        <temperatureDependence>
            <text evidence="2">Optimum temperature is 70 degrees Celsius.</text>
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    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="2">Consists of two domains, an N-terminal nucleotide-binding domain and a C-terminal dimerization domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
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    <sequence length="354" mass="39439" checksum="5C412CA5C39B166E" modified="1997-11-01" version="1">MSKGEKMKIKVGVLGATGSVGQRFVQLLADHPMFELTALAASERSAGKKYKDACYWFQDRDIPENIKDMVVIPTDPKHEEFEDVDIVFSALPSDLAKKFEPEFAKEGKLIFSNASAYRMEEDVPLVIPEVNADHLELIEIQREKRGWDGAIITNPNCSTICAVITLKPIMDKFGLEAVFIATMQAVSGAGYNGVPSMAILDNLIPFIKNEEEKMQTESLKLLGTLKDGKVELANFKISASCNRVAVIDGHTESIFVKTKEGAEPEEIKEVMDKFDPLKDLNLPTYAKPIVIREEIDRPQPRLDRNEGNGMSIVVGRIRKDPIFDVKYTALEHNTIRGAAGASVLNAEYFVKKYI</sequence>
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<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q57676</accession>
    <name>Y223_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ0223</fullName>
        </recommendedName>
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    <gene>
        <name type="ordered locus">MJ0223</name>
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    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64327">
        <property type="entry name" value="H64327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869719.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2K6I">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=3-46"/>
    </dbReference>
    <dbReference type="PDBsum" id="2K6I"/>
    <dbReference type="AlphaFoldDB" id="Q57676"/>
    <dbReference type="BMRB" id="Q57676"/>
    <dbReference type="SMR" id="Q57676"/>
    <dbReference type="STRING" id="243232.MJ_0223"/>
    <dbReference type="EnsemblBacteria" id="AAB98215">
        <property type="protein sequence ID" value="AAB98215"/>
        <property type="gene ID" value="MJ_0223"/>
    </dbReference>
    <dbReference type="GeneID" id="1451073"/>
    <dbReference type="KEGG" id="mja:MJ_0223"/>
    <dbReference type="eggNOG" id="arCOG03363">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177461_0_0_2"/>
    <dbReference type="OMA" id="TIERWKE"/>
    <dbReference type="EvolutionaryTrace" id="Q57676"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014275">
        <property type="entry name" value="ATPase_A1A0-cplx_hsu"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02926">
        <property type="entry name" value="AhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106746" description="Uncharacterized protein MJ0223">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="6"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="14"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="2K6I"/>
        </source>
    </evidence>
    <sequence length="104" mass="11805" checksum="9BB4DED252DF0732" modified="1996-11-01" version="1">MSVSVMEAIKEVKLAEEQAVKEIEEAKNRAEQIKAEAIEEAKKLIAEAEEEAKKLVEEMIKKAEEEAKKEAEKILEETEKEIKEIISIAKVKILSLKLSEILEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57683</accession>
    <name>Y230_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0230</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0230</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64328">
        <property type="entry name" value="G64328"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57683"/>
    <dbReference type="SMR" id="Q57683"/>
    <dbReference type="STRING" id="243232.MJ_0230"/>
    <dbReference type="EnsemblBacteria" id="AAB98216">
        <property type="protein sequence ID" value="AAB98216"/>
        <property type="gene ID" value="MJ_0230"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0230"/>
    <dbReference type="eggNOG" id="arCOG05236">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2476020_0_0_2"/>
    <dbReference type="OMA" id="PNTDNER"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106751" description="Uncharacterized protein MJ0230">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="87" mass="10070" checksum="C4185109EA09D52F" modified="1996-11-01" version="1">MFVGEIMPMGFGVHYVGSEGVAINPFYDILWMIIFVVIIAVIIYILISPLKKQSSSIDNEKLIKIEKDVEEIKEIVKELKKKWEEIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57693</accession>
    <name>Y241_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0241</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0241</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64330">
        <property type="entry name" value="B64330"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869739.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57693"/>
    <dbReference type="SMR" id="Q57693"/>
    <dbReference type="STRING" id="243232.MJ_0241"/>
    <dbReference type="EnsemblBacteria" id="AAB98229">
        <property type="protein sequence ID" value="AAB98229"/>
        <property type="gene ID" value="MJ_0241"/>
    </dbReference>
    <dbReference type="GeneID" id="1451095"/>
    <dbReference type="KEGG" id="mja:MJ_0241"/>
    <dbReference type="eggNOG" id="arCOG00814">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792142_0_0_2"/>
    <dbReference type="OMA" id="IWLVIEN"/>
    <dbReference type="OrthoDB" id="94149at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030489">
        <property type="entry name" value="TR_Rrf2-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000944">
        <property type="entry name" value="Tscrpt_reg_Rrf2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33221">
        <property type="entry name" value="PTHR33221"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02082">
        <property type="entry name" value="Rrf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106758" description="Uncharacterized protein MJ0241">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="78"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01007"/>
        </source>
    </evidence>
    <sequence length="145" mass="16280" checksum="D55F5A96CCF28534" modified="1996-11-01" version="1">MKVVGLTKKIMEHLKEPITIKELAKKLNMHPKNLDVKIRVLRDLGLVETKKGRNGGVRLTKEGLYLLEKGEITLGSLKLQIVAKDRIGLLADITSRISKIGGNITSTVLEREGDEVIIYLVVENVDKDEIKNTLEDVVEKISILW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57711</accession>
    <name>Y941_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TPR repeat-containing protein MJ0941</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0941</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64417">
        <property type="entry name" value="E64417"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57711"/>
    <dbReference type="SMR" id="Q57711"/>
    <dbReference type="STRING" id="243232.MJ_0941"/>
    <dbReference type="EnsemblBacteria" id="AAB98947">
        <property type="protein sequence ID" value="AAB98947"/>
        <property type="gene ID" value="MJ_0941"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0941"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003728_2_5_2"/>
    <dbReference type="InParanoid" id="Q57711"/>
    <dbReference type="OMA" id="GAYGKAH"/>
    <dbReference type="PhylomeDB" id="Q57711"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0802">TPR repeat</keyword>
    <feature type="chain" id="PRO_0000106459" description="TPR repeat-containing protein MJ0941">
        <location>
            <begin position="1"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 1">
        <location>
            <begin position="27"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 2">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 3">
        <location>
            <begin position="97"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 4">
        <location>
            <begin position="131"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 5">
        <location>
            <begin position="165"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 6">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 7">
        <location>
            <begin position="268"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 8">
        <location>
            <begin position="302"/>
            <end position="335"/>
        </location>
    </feature>
    <sequence length="338" mass="39450" checksum="0A6A40E2FCDDBBE7" modified="1998-12-15" version="3">MIDKIIILYFYGDMRMNRKRTPRVDTLEAVANVLRAYRELFEGNLIKALYYVDKALELEPDFYLALFLKGLALSAKGEIKEAITTFEELLSYESKNPITWVFVGQLYGMSGNCDEALKCYNKALGIENRFLSAFLLKTICLEFLGEYDELLKCYNEVLTYTPNFVPMWVKKAEILRKLGRYEDALLCLNRALELKPHDKNALYLKGVLLKRMGKFREALECFKKLIDELNVKWIDAIRHAVSLMLALDDLKDAERYINIGLEIRKDDVALWYFKGELYERLGKLDEALKCYEKVIELQPHYIKALLSKARIYERQGNIEAAIEYYNKAVENIHKDHGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q57725</accession>
    <name>ILVB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable acetolactate synthase large subunit</fullName>
            <shortName>AHAS</shortName>
            <ecNumber>2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Acetohydroxy-acid synthase large subunit</fullName>
            <shortName>ALS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ilvB</name>
        <name type="ordered locus">MJ0277</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>H(+) + 2 pyruvate = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="1">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64334">
        <property type="entry name" value="F64334"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869775.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57725"/>
    <dbReference type="SMR" id="Q57725"/>
    <dbReference type="STRING" id="243232.MJ_0277"/>
    <dbReference type="EnsemblBacteria" id="AAB98265">
        <property type="protein sequence ID" value="AAB98265"/>
        <property type="gene ID" value="MJ_0277"/>
    </dbReference>
    <dbReference type="GeneID" id="1451132"/>
    <dbReference type="KEGG" id="mja:MJ_0277"/>
    <dbReference type="eggNOG" id="arCOG01998">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_1_3_2"/>
    <dbReference type="InParanoid" id="Q57725"/>
    <dbReference type="OMA" id="CFGTSGP"/>
    <dbReference type="OrthoDB" id="7134at2157"/>
    <dbReference type="PhylomeDB" id="Q57725"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02015">
        <property type="entry name" value="TPP_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012846">
        <property type="entry name" value="Acetolactate_synth_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039368">
        <property type="entry name" value="AHAS_TPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000399">
        <property type="entry name" value="TPP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00118">
        <property type="entry name" value="acolac_lg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00187">
        <property type="entry name" value="TPP_ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000090808" description="Probable acetolactate synthase large subunit">
        <location>
            <begin position="1"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine pyrophosphate binding">
        <location>
            <begin position="396"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="258"/>
            <end position="279"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="591" mass="64493" checksum="DB398C926D9B3A9D" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q57733</accession>
    <name>HSPS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Small heat shock protein HSP16.5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0285</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Proc. Natl. Acad. Sci. U.S.A." volume="95" first="9129" last="9133">
            <title>Small heat shock protein of Methanococcus jannaschii, a hyperthermophile.</title>
            <authorList>
                <person name="Kim R."/>
                <person name="Kim K.K."/>
                <person name="Yokota H."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="9689045"/>
            <dbReference type="DOI" id="10.1073/pnas.95.16.9129"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1998" name="Nature" volume="394" first="595" last="599">
            <title>Crystal structure of a small heat-shock protein.</title>
            <authorList>
                <person name="Kim K.K."/>
                <person name="Kim R."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="9707123"/>
            <dbReference type="DOI" id="10.1038/29106"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS)</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Chaperone that confers thermal protection to other proteins.</text>
    </comment>
    <comment type="subunit">
        <text>Homooligomer of 24 subunits. Forms a spherical shape.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-15555697">
            <id>Q57733</id>
        </interactant>
        <interactant intactId="EBI-15555697">
            <id>Q57733</id>
            <label>MJ0285</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>2</experiments>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64335">
        <property type="entry name" value="F64335"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869783.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1SHS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-147"/>
    </dbReference>
    <dbReference type="PDB" id="4ELD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1-147"/>
    </dbReference>
    <dbReference type="PDB" id="4I88">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.85 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-147"/>
    </dbReference>
    <dbReference type="PDBsum" id="1SHS"/>
    <dbReference type="PDBsum" id="4ELD"/>
    <dbReference type="PDBsum" id="4I88"/>
    <dbReference type="AlphaFoldDB" id="Q57733"/>
    <dbReference type="SMR" id="Q57733"/>
    <dbReference type="DIP" id="DIP-48450N"/>
    <dbReference type="STRING" id="243232.MJ_0285"/>
    <dbReference type="EnsemblBacteria" id="AAB98273">
        <property type="protein sequence ID" value="AAB98273"/>
        <property type="gene ID" value="MJ_0285"/>
    </dbReference>
    <dbReference type="GeneID" id="1451140"/>
    <dbReference type="KEGG" id="mja:MJ_0285"/>
    <dbReference type="eggNOG" id="arCOG01832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046737_12_4_2"/>
    <dbReference type="InParanoid" id="Q57733"/>
    <dbReference type="OMA" id="SGKGFMP"/>
    <dbReference type="OrthoDB" id="100198at2157"/>
    <dbReference type="PhylomeDB" id="Q57733"/>
    <dbReference type="EvolutionaryTrace" id="Q57733"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043621">
        <property type="term" value="F:protein self-association"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="DisProt" id="DP00067"/>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="SSF49764"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0346">Stress response</keyword>
    <feature type="chain" id="PRO_0000126060" description="Small heat shock protein HSP16.5">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="sHSP" evidence="1">
        <location>
            <begin position="36"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="36"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="45"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="51"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="65"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="68"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="76"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="93"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="104"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="121"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="128"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="4ELD"/>
        </source>
    </evidence>
    <sequence length="147" mass="16452" checksum="0A53C6981CFD4266" modified="1996-11-01" version="1">MFGRDPFDSLFERMFKEFFATPMTGTTMIQSSTGIQISGKGFMPISIIEGDQHIKVIAWLPGVNKEDIILNAVGDTLEIRAKRSPLMITESERIIYSEIPEEEEIYRTIKLPATVKEENASAKFENGVLSVILPKAESSIKKGINIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57734</accession>
    <name>Y286_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0286</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0286</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64335">
        <property type="entry name" value="G64335"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869784.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57734"/>
    <dbReference type="STRING" id="243232.MJ_0286"/>
    <dbReference type="EnsemblBacteria" id="AAB98275">
        <property type="protein sequence ID" value="AAB98275"/>
        <property type="gene ID" value="MJ_0286"/>
    </dbReference>
    <dbReference type="GeneID" id="1451141"/>
    <dbReference type="KEGG" id="mja:MJ_0286"/>
    <dbReference type="eggNOG" id="arCOG09661">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2079428_0_0_2"/>
    <dbReference type="OMA" id="EIKWISM"/>
    <dbReference type="OrthoDB" id="111685at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106773" description="Uncharacterized protein MJ0286">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <sequence length="117" mass="14099" checksum="09DCBDDD263919C5" modified="1996-11-01" version="1">MKKFGTVLLSDIVKECLSGDEFAREMMEDLFNFLIKLRLWRWKYLLSQNQKNEIQMSDLLALIKEEKEGINRLFSFLYQTDIPVENRIEILMLLKEFVKEEIKWISMDVSEINFVKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57842</accession>
    <name>MANB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Phosphomannomutase</fullName>
            <shortName>PMM</shortName>
            <ecNumber evidence="2">5.4.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Phosphoglucomutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">manB</name>
        <name type="ordered locus">MJ0399</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Biochemistry" volume="45" first="12366" last="12379">
            <title>Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="White R.H."/>
                <person name="Xu H."/>
            </authorList>
            <dbReference type="PubMed" id="17014089"/>
            <dbReference type="DOI" id="10.1021/bi061018a"/>
        </citation>
        <scope>FUNCTION AS A PHOSPHOMANNOMUTASE</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the formation of mannose-1-P from mannose-6-P. Can also use glucose-6-P.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="2">
            <dbReference type="Rhea" id="RHEA:11142"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64349">
        <property type="entry name" value="G64349"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57842"/>
    <dbReference type="SMR" id="Q57842"/>
    <dbReference type="STRING" id="243232.MJ_0399"/>
    <dbReference type="EnsemblBacteria" id="AAB98388">
        <property type="protein sequence ID" value="AAB98388"/>
        <property type="gene ID" value="MJ_0399"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0399"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_9_2_2"/>
    <dbReference type="InParanoid" id="Q57842"/>
    <dbReference type="OMA" id="HSGEINF"/>
    <dbReference type="PhylomeDB" id="Q57842"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147831" description="Phosphomannomutase">
        <location>
            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P26276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17014089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="17014089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="17014089"/>
        </source>
    </evidence>
    <sequence length="449" mass="51474" checksum="1EB07EEAB409BA00" modified="1996-11-01" version="1">MFGDLMVFKAYDIRGIYGRELDENFAYSLGKCIGKKFENKKILVGNDVRIGSKELLPYFIVGLKEYADVFYAGTISTPLMYFGTKGKYDLGVILTASHNPPEYTGFKMCDKEAIPLSPIEEIKPIFKKYELTESIKEEAKNLNLDDLKVNIIEEYKKFFLKRCKASDKKIAVDFANGATTIAEKEILNELFDNAVFINDYPDGNFPAHQPDTLKMECLKDIIRAVKKNNCELGLIFDGDGDRLGIVDENGNVLRGDILTAIIAKEILKEKSNAKIVYDLRCSKIVPEIIEKYGGIAIKSRVGHYFIKKLMHEIDAEFAGELSNHFYFKEIGYFESPLLALNYILKAMDEENKSLSELNKEFSKYPHSGEINFRVKDQKYIMEKIKEHFKDCKLEELDGISIYCKNFWFNLRPSNTEPLLRLNLEADDEKTMKEKVEEIKNLIAKLDASL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q57869</accession>
    <name>Y426_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0426</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0426</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64353">
        <property type="entry name" value="B64353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869925.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57869"/>
    <dbReference type="STRING" id="243232.MJ_0426"/>
    <dbReference type="EnsemblBacteria" id="AAB98419">
        <property type="protein sequence ID" value="AAB98419"/>
        <property type="gene ID" value="MJ_0426"/>
    </dbReference>
    <dbReference type="GeneID" id="1451286"/>
    <dbReference type="KEGG" id="mja:MJ_0426"/>
    <dbReference type="eggNOG" id="arCOG08292">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1507409_0_0_2"/>
    <dbReference type="OMA" id="YALYIDW"/>
    <dbReference type="OrthoDB" id="93767at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106871" description="Uncharacterized protein MJ0426">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <sequence length="180" mass="21609" checksum="E9BE603DEBAE4053" modified="1996-11-01" version="1">MIMKELIKILKQFGIYSEYLKILDVELDGDRYITILIPTTLNWIEEEEIEEILEEMLKNVRVKISRLPLNKFIKIYLEKNVKNKAYGENIENVKIEGENYALYIDWKNKKIVIHKFNGKNPIKESCKLSSNWETMWGIWVLGFESKEKAKEFAENLADEIYKYYEIDFDIEEHKRCLEDN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q57877</accession>
    <name>Y435_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ0435</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ0435</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0435</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable antitoxin component of a putative type VII toxin-antitoxin (TA) system. Neutralizes cognate toxic MJ0434 by di-AMPylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Probably forms a complex with cognate toxin MJ0434.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57877"/>
    <dbReference type="SMR" id="Q57877"/>
    <dbReference type="STRING" id="243232.MJ_0435"/>
    <dbReference type="PRIDE" id="Q57877"/>
    <dbReference type="EnsemblBacteria" id="AAB98423">
        <property type="protein sequence ID" value="AAB98423"/>
        <property type="gene ID" value="MJ_0435"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0435"/>
    <dbReference type="eggNOG" id="arCOG01206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_4_1_2"/>
    <dbReference type="InParanoid" id="Q57877"/>
    <dbReference type="OMA" id="YNSIHPL"/>
    <dbReference type="PhylomeDB" id="Q57877"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041633">
        <property type="entry name" value="Polbeta"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18765">
        <property type="entry name" value="Polbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106875" description="Putative protein adenylyltransferase MJ0435">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="93" mass="10661" checksum="AA6FD2014B942A68" modified="1998-12-15" version="2">MNINEIKRKIIPILLKHGVKRASIFGSYARNEQKETSDIDILVEFGEGKSLLDLVRLKYELEEVLGKEVDVLTYNSIHPLLKDRILNEAVDVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q57901</accession>
    <name>EF1B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Elongation factor 1-beta</fullName>
            <shortName>EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ef1b</name>
        <name type="ordered locus">MJ0459</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64357">
        <property type="entry name" value="C64357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869959.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57901"/>
    <dbReference type="SMR" id="Q57901"/>
    <dbReference type="STRING" id="243232.MJ_0459"/>
    <dbReference type="EnsemblBacteria" id="AAB98467">
        <property type="protein sequence ID" value="AAB98467"/>
        <property type="gene ID" value="MJ_0459"/>
    </dbReference>
    <dbReference type="GeneID" id="1451321"/>
    <dbReference type="KEGG" id="mja:MJ_0459"/>
    <dbReference type="eggNOG" id="arCOG01988">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165896_0_0_2"/>
    <dbReference type="InParanoid" id="Q57901"/>
    <dbReference type="OMA" id="VMPNSPE"/>
    <dbReference type="OrthoDB" id="124073at2157"/>
    <dbReference type="PhylomeDB" id="Q57901"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribosomal_S6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="PTHR39647"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="SSF54984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0251">Elongation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155058" description="Elongation factor 1-beta">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="89" mass="9921" checksum="0153FB4067D36980" modified="1996-11-01" version="1">MATVLAKIKIMPTSPEVNKEKLKEKIKEVLEKQDVAIRGLFDEPLAFGLYAIYTVIEMEEREGGTEPIENALAEIDEVESVETVEVSLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q57923</accession>
    <name>Y500_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0500</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0500</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64362">
        <property type="entry name" value="D64362"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57923"/>
    <dbReference type="SMR" id="Q57923"/>
    <dbReference type="STRING" id="243232.MJ_0500"/>
    <dbReference type="EnsemblBacteria" id="AAB98491">
        <property type="protein sequence ID" value="AAB98491"/>
        <property type="gene ID" value="MJ_0500"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0500"/>
    <dbReference type="eggNOG" id="arCOG03216">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094096_0_0_2"/>
    <dbReference type="InParanoid" id="Q57923"/>
    <dbReference type="OMA" id="THLASMK"/>
    <dbReference type="PhylomeDB" id="Q57923"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106901" description="Uncharacterized protein MJ0500">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <sequence length="259" mass="29193" checksum="5C528A176B1C2D36" modified="1996-11-01" version="1">MSFMIIEEIKERALNLLSEKEEDFKVIDFSFALPYSYVLIESNGKKALGVAMTLLEEYRGHGNRKDLNINKNLEEFINMADSFDIVERTLGVAAINAVSQYYFNFEANGKDAAELVLNRDDIKKIAFVGNMIPVVNMLKKSEKFDIYVFERSPSLLMDGVLSDAFEYRLLPEMDAVFISGTTLLNDTLDFVLDRAKNAKLKILVGPTAQSLPELFKGFGITHIASTKIIDVDKALLYLKFASSSMLFKGASKKYTMEVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q57924</accession>
    <name>Y501_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0501</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0501</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64362">
        <property type="entry name" value="E64362"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57924"/>
    <dbReference type="STRING" id="243232.MJ_0501"/>
    <dbReference type="EnsemblBacteria" id="AAB98492">
        <property type="protein sequence ID" value="AAB98492"/>
        <property type="gene ID" value="MJ_0501"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0501"/>
    <dbReference type="eggNOG" id="arCOG04894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087505_0_0_2"/>
    <dbReference type="InParanoid" id="Q57924"/>
    <dbReference type="OMA" id="PEFYFEI"/>
    <dbReference type="PhylomeDB" id="Q57924"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019218">
        <property type="entry name" value="DUF2119"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09892">
        <property type="entry name" value="DUF2119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005919">
        <property type="entry name" value="UCP005919"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106902" description="Uncharacterized protein MJ0501">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <sequence length="202" mass="23743" checksum="8F407212641089A5" modified="1996-11-01" version="1">MKIMEIFEFKGNGVKKLFIGGLHGNEGKFTEIILKDFVNSLKECNYIGDIVVIPKLVENSKYISTLSEKYYESDEGKTLINIIKKYKPKVYFELHAYKKENYKKLTSNNRKKVPPLIDIGNNVLIASISPILRKRFSKEDFCMTIEIPSWKVYEVKDEILKILKIGAESLRREEIIEKLKKIYPEHIEKAEYFSKKYNLMLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q57927</accession>
    <name>Y504_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0504</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0504</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64362">
        <property type="entry name" value="H64362"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870005.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57927"/>
    <dbReference type="STRING" id="243232.MJ_0504"/>
    <dbReference type="EnsemblBacteria" id="AAB98495">
        <property type="protein sequence ID" value="AAB98495"/>
        <property type="gene ID" value="MJ_0504"/>
    </dbReference>
    <dbReference type="GeneID" id="1451366"/>
    <dbReference type="KEGG" id="mja:MJ_0504"/>
    <dbReference type="eggNOG" id="arCOG04343">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095207_1_0_2"/>
    <dbReference type="InParanoid" id="Q57927"/>
    <dbReference type="OMA" id="VTYHQGF"/>
    <dbReference type="OrthoDB" id="104210at2157"/>
    <dbReference type="PhylomeDB" id="Q57927"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015074">
        <property type="entry name" value="DUF1867"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016138">
        <property type="entry name" value="UCP016138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106903" description="Uncharacterized protein MJ0504">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <sequence length="196" mass="21452" checksum="F994E98F4670196B" modified="1996-11-01" version="1">MKLFDYPGIQNTDETLKIAVERAKKGDIKSIVVASSTGYTAKKLLDLLEKEGLDLNVVVVTYHQGFHGEDTISMDKEVEEELKKRGAKVFRGSHALSGVERGISNKLGGYGPVQVIAETLRTFGQGVKVCYEITIMACDAGLIKAKEEVIAIGGTGRGADTAMVIKPANMNTFFNIEAREILCMPRIKKKVKEDDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q57936</accession>
    <name>Y516_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0516</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0516</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870019.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57936"/>
    <dbReference type="SMR" id="Q57936"/>
    <dbReference type="STRING" id="243232.MJ_0516"/>
    <dbReference type="EnsemblBacteria" id="AAB98505">
        <property type="protein sequence ID" value="AAB98505"/>
        <property type="gene ID" value="MJ_0516"/>
    </dbReference>
    <dbReference type="GeneID" id="1451380"/>
    <dbReference type="KEGG" id="mja:MJ_0516"/>
    <dbReference type="eggNOG" id="arCOG01553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="InParanoid" id="Q57936"/>
    <dbReference type="OMA" id="QYGIYVH"/>
    <dbReference type="OrthoDB" id="100307at2157"/>
    <dbReference type="PhylomeDB" id="Q57936"/>
    <dbReference type="BRENDA" id="2.7.1.78">
        <property type="organism ID" value="5244"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000118783" description="Uncharacterized protein MJ0516">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="117"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="148" mass="16276" checksum="6EF752B2509B9C94" modified="1998-06-01" version="2">MMKELFRKRSIHVCVVNTGGCNGCDIEIVACLAPRYDIEQYGIYVHNNPREADVLLVTGPVTLQWAERLKEIYEKTPEPKIVVAVGACALSGGIFKEGHVVGGVDKVIPVDAKIPGCPPRPSEIIETILKVAPKAIAMREKRLKNKDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q57946</accession>
    <name>Y526_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0526</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0526</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64365">
        <property type="entry name" value="F64365"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870029.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57946"/>
    <dbReference type="SMR" id="Q57946"/>
    <dbReference type="STRING" id="243232.MJ_0526"/>
    <dbReference type="EnsemblBacteria" id="AAB98529">
        <property type="protein sequence ID" value="AAB98529"/>
        <property type="gene ID" value="MJ_0526"/>
    </dbReference>
    <dbReference type="GeneID" id="1451390"/>
    <dbReference type="KEGG" id="mja:MJ_0526"/>
    <dbReference type="eggNOG" id="arCOG04829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170806_0_0_2"/>
    <dbReference type="OMA" id="LEIGMIG"/>
    <dbReference type="OrthoDB" id="124851at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019213">
        <property type="entry name" value="DUF2108_membrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011308">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09881">
        <property type="entry name" value="DUF2108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006581">
        <property type="entry name" value="EhaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106915" description="Uncharacterized protein MJ0526">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="92" mass="10077" checksum="CB4935AF56AA3110" modified="1996-11-01" version="1">MEVLPLVSGICCILGGIGVILHTNPINKIIMLALLEIGMIGLIVSCYYLDIAIVSSLCEPICTVILLLGYLKYLTTVKKKKRYGRNLPILSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q57961</accession>
    <name>NADM_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber>2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0541</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="J. Bacteriol." volume="179" first="7718" last="7723">
            <title>Characterization of nicotinamide mononucleotide adenylyltransferase from thermophilic archaea.</title>
            <authorList>
                <person name="Raffaelli N."/>
                <person name="Pisani F.M."/>
                <person name="Lorenzi T."/>
                <person name="Emanuelli M."/>
                <person name="Amici A."/>
                <person name="Ruggieri S."/>
                <person name="Magni G."/>
            </authorList>
            <dbReference type="PubMed" id="9401030"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 2-6</scope>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1999" name="Mol. Cell. Biochem." volume="193" first="99" last="102">
            <title>Identification of the archaeal NMN adenylytransferase gene.</title>
            <authorList>
                <person name="Raffaelli N."/>
                <person name="Emanuelli M."/>
                <person name="Pisani F.M."/>
                <person name="Amici A."/>
                <person name="Lorenzi T."/>
                <person name="Ruggieri S."/>
                <person name="Magni G."/>
            </authorList>
            <dbReference type="PubMed" id="10331644"/>
            <dbReference type="DOI" id="10.1023/A:1006968328186"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2000" name="Structure" volume="8" first="993" last="1004">
            <title>Structure of nicotinamide mononucleotide adenylyltransferase: a key enzyme in NAD(+) biosynthesis.</title>
            <authorList>
                <person name="D'Angelo I."/>
                <person name="Raffaelli N."/>
                <person name="Dabusti V."/>
                <person name="Lorenzi T."/>
                <person name="Magni G."/>
                <person name="Rizzi M."/>
            </authorList>
            <dbReference type="PubMed" id="10986466"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(00)00190-8"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2 ANGSTROMS)</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + beta-nicotinamide D-ribonucleotide + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text>Homohexamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64367">
        <property type="entry name" value="E64367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870045.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1F9A">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D/E/F=2-168"/>
    </dbReference>
    <dbReference type="PDBsum" id="1F9A"/>
    <dbReference type="AlphaFoldDB" id="Q57961"/>
    <dbReference type="SMR" id="Q57961"/>
    <dbReference type="STRING" id="243232.MJ_0541"/>
    <dbReference type="PRIDE" id="Q57961"/>
    <dbReference type="EnsemblBacteria" id="AAB98533">
        <property type="protein sequence ID" value="AAB98533"/>
        <property type="gene ID" value="MJ_0541"/>
    </dbReference>
    <dbReference type="GeneID" id="1451406"/>
    <dbReference type="KEGG" id="mja:MJ_0541"/>
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    <dbReference type="BRENDA" id="2.7.7.1">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57961"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000134992" description="Nicotinamide-nucleotide adenylyltransferase">
        <location>
            <begin position="2"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="28"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="41"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="66"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="78"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="81"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="100"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="156"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0000305" key="2">
        <source>
            <dbReference type="PubMed" id="9401030"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1F9A"/>
        </source>
    </evidence>
    <sequence length="168" mass="19610" checksum="D82FF45E68E29834" modified="2007-01-23" version="3">MRGFIIGRFQPFHKGHLEVIKKIAEEVDEIIIGIGSAQKSHTLENPFTAGERILMITQSLKDYDLTYYPIPIKDIEFNSIWVSYVESLTPPFDIVYSGNPLVRVLFEERGYEVKRPEMFNRKEYSGTEIRRRMLNGEKWEHLVPKAVVDVIKEIKGVERLRKLAQTDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q57970</accession>
    <name>Y550_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0550</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0550</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64368">
        <property type="entry name" value="F64368"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870054.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57970"/>
    <dbReference type="SMR" id="Q57970"/>
    <dbReference type="STRING" id="243232.MJ_0550"/>
    <dbReference type="PRIDE" id="Q57970"/>
    <dbReference type="EnsemblBacteria" id="AAB98542">
        <property type="protein sequence ID" value="AAB98542"/>
        <property type="gene ID" value="MJ_0550"/>
    </dbReference>
    <dbReference type="GeneID" id="1451415"/>
    <dbReference type="KEGG" id="mja:MJ_0550"/>
    <dbReference type="eggNOG" id="arCOG01360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060488_0_0_2"/>
    <dbReference type="InParanoid" id="Q57970"/>
    <dbReference type="OMA" id="KRGPHNC"/>
    <dbReference type="OrthoDB" id="29724at2157"/>
    <dbReference type="PhylomeDB" id="Q57970"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005909">
        <property type="entry name" value="RaSEA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="PTHR11135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004954">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01210">
        <property type="entry name" value="TIGR01210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000106927" description="Uncharacterized protein MJ0550">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="45"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="365" mass="42518" checksum="ED684F9589D77411" modified="1996-11-01" version="1">MNQIINDNYREFLKKLRERHLKKRKVKDKNKPIAVWMQDDIYRDFSIGKSLTIILRTEGCYYAKEGGCLMCSYLMDSSPEKITAENIINQFNYAIEKYKEKIKDLKDFSVKIFTSGSFLDDREVPKEARNYIFKKLSEFDNLKEVAIESRPEFIDEDKLNEIRKYLDVNVEIGVGIESFNEEIREKAINKGITNEQIIRAIELAKNYNIGIKAYLLIKPLFITEKEAIYDSISSANKCIELGCSRISFCPATVHKGSVMEFFFNKNQYRPPFLWSIIEILKEVKKSNPKALIMCDTSGVGSERGAHNLYNCKCNKLIKERLERFTLTQDINVLNVECECKNIWNAYIEVENKNIVPLGDERKLLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q57975</accession>
    <name>Y555_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative aminopeptidase MJ0555</fullName>
            <ecNumber>3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0555</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M42 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64369">
        <property type="entry name" value="C64369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870059.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57975"/>
    <dbReference type="SMR" id="Q57975"/>
    <dbReference type="STRING" id="243232.MJ_0555"/>
    <dbReference type="EnsemblBacteria" id="AAB98546">
        <property type="protein sequence ID" value="AAB98546"/>
        <property type="gene ID" value="MJ_0555"/>
    </dbReference>
    <dbReference type="GeneID" id="1451420"/>
    <dbReference type="KEGG" id="mja:MJ_0555"/>
    <dbReference type="eggNOG" id="arCOG01518">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047249_1_0_2"/>
    <dbReference type="InParanoid" id="Q57975"/>
    <dbReference type="OMA" id="FGWPAIH"/>
    <dbReference type="OrthoDB" id="27966at2157"/>
    <dbReference type="PhylomeDB" id="Q57975"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008007">
        <property type="entry name" value="Peptidase_M42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023367">
        <property type="entry name" value="Peptidase_M42_dom2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05343">
        <property type="entry name" value="Peptidase_M42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001123">
        <property type="entry name" value="PepA_GA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000071660" description="Putative aminopeptidase MJ0555">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="350" mass="38622" checksum="9D4942EF5528955B" modified="1997-11-01" version="1">MSVVEYLKKLSKLHGISGREDSVREFMKKELEKYCDSVEIDNFGNLIAKRGNKGKKIMIAAHMDEIGLMVKYIDDNGFLKFTKIGGIYDPTILNQKVVVHGSKGDLIGVLGSKPPHRMKEEEKTKIIKYEDMFIDIGAESREEAIEMGVNIGTWVSFLSEVYDLGKNRLTGKAFDDRVGCAVLLEVMKRLSEEDIDCQVYAVGTVQEEVGLKGARVSAFKINPDVAIALDVTIAGDHPGIKKEDAPVDLGKGPVVGIVDASGRGLIAHPKVLDMIKAVSEKYKIDVQWEVGEGGTTDATAIHLTREGIPTGVISVPARYIHTPVEVIDKRDLEKTVELVYNCIKEVNNFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q57984</accession>
    <name>SYA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Alanine--tRNA ligase</fullName>
            <ecNumber>6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Alanyl-tRNA synthetase</fullName>
            <shortName>AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">alaS</name>
        <name type="ordered locus">MJ0564</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Proc. Natl. Acad. Sci. U.S.A." volume="100" first="15422" last="15427">
            <title>Trans-editing of mischarged tRNAs.</title>
            <authorList>
                <person name="Ahel I."/>
                <person name="Korencic D."/>
                <person name="Ibba M."/>
                <person name="Soll D."/>
            </authorList>
            <dbReference type="PubMed" id="14663147"/>
            <dbReference type="DOI" id="10.1073/pnas.2136934100"/>
        </citation>
        <scope>FUNCTION IN EDITING ACTIVITY ON MISCHARGED TRNA(ALA)</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala) (By similarity). Also functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala), and maybe also from Gly-tRNA(Ala).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64370">
        <property type="entry name" value="D64370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870068.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57984"/>
    <dbReference type="SMR" id="Q57984"/>
    <dbReference type="STRING" id="243232.MJ_0564"/>
    <dbReference type="PRIDE" id="Q57984"/>
    <dbReference type="DNASU" id="1451429"/>
    <dbReference type="EnsemblBacteria" id="AAB98556">
        <property type="protein sequence ID" value="AAB98556"/>
        <property type="gene ID" value="MJ_0564"/>
    </dbReference>
    <dbReference type="GeneID" id="1451429"/>
    <dbReference type="KEGG" id="mja:MJ_0564"/>
    <dbReference type="eggNOG" id="arCOG01255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004485_4_0_2"/>
    <dbReference type="InParanoid" id="Q57984"/>
    <dbReference type="OMA" id="YHHTMFE"/>
    <dbReference type="OrthoDB" id="7896at2157"/>
    <dbReference type="PhylomeDB" id="Q57984"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="SSF101353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="SSF55186"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000075264" description="Alanine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="892"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="590"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="594"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="699"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="703"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="14663147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="892" mass="102851" checksum="409A542E72C8188B" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q57995</accession>
    <name>Y575_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0575</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0575</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0838.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64371">
        <property type="entry name" value="G64371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870079.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57995"/>
    <dbReference type="STRING" id="243232.MJ_0575"/>
    <dbReference type="PRIDE" id="Q57995"/>
    <dbReference type="DNASU" id="1451440"/>
    <dbReference type="EnsemblBacteria" id="AAB98566">
        <property type="protein sequence ID" value="AAB98566"/>
        <property type="gene ID" value="MJ_0575"/>
    </dbReference>
    <dbReference type="GeneID" id="1451440"/>
    <dbReference type="KEGG" id="mja:MJ_0575"/>
    <dbReference type="eggNOG" id="arCOG02469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110109_0_0_2"/>
    <dbReference type="InParanoid" id="Q57995"/>
    <dbReference type="OMA" id="EMCLLDE"/>
    <dbReference type="OrthoDB" id="56837at2157"/>
    <dbReference type="PhylomeDB" id="Q57995"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106938" description="Uncharacterized protein MJ0575">
        <location>
            <begin position="1"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="229" mass="26218" checksum="32A33DF47C2E5BD3" modified="1996-11-01" version="1">MAKILVVTDGAYGYRIKGTINSFGKKNKFIGIYKINKPDDLIVDDIEFPDELLEKIKEADILLLYTQHPDNTYYLCYEARRLNKDIAIIVATWSGEGEKKELKKFDAICPEEMCLLDENEVGSLIDKYPKLKEFLEEFGTPKVKVYVKDNKVIDVEVLRTSICGSTLFMAKLMKGMEVNDIEEFAKKSAMLIQRYPCVAGKIKIFRGDCRKQKALNIHKEAVLEGIIFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57996</accession>
    <name>Y576_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter MJ0576</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0576</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tellurite-resistance/dicarboxylate transporter (TDT) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64371">
        <property type="entry name" value="H64371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870080.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57996"/>
    <dbReference type="SMR" id="Q57996"/>
    <dbReference type="STRING" id="243232.MJ_0576"/>
    <dbReference type="EnsemblBacteria" id="AAB98567">
        <property type="protein sequence ID" value="AAB98567"/>
        <property type="gene ID" value="MJ_0576"/>
    </dbReference>
    <dbReference type="GeneID" id="1451441"/>
    <dbReference type="KEGG" id="mja:MJ_0576"/>
    <dbReference type="eggNOG" id="arCOG04355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030057_6_4_2"/>
    <dbReference type="InParanoid" id="Q57996"/>
    <dbReference type="OMA" id="PVQSMFI"/>
    <dbReference type="OrthoDB" id="47545at2157"/>
    <dbReference type="PhylomeDB" id="Q57996"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000319">
        <property type="term" value="F:sulfite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000316">
        <property type="term" value="P:sulfite transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004695">
        <property type="entry name" value="SLAC1/Mae1/Ssu1/TehA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011552">
        <property type="entry name" value="TehA/Mae1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038665">
        <property type="entry name" value="Voltage-dep_anion_channel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03595">
        <property type="entry name" value="SLAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00816">
        <property type="entry name" value="tdt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000106939" description="Uncharacterized transporter MJ0576">
        <location>
            <begin position="1"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="347" mass="39557" checksum="632F7671A31DE183" modified="1996-11-01" version="1">MLEACESKLDIIKNFVPSWFAAVMGTGILAVDSLLYSSYLPILKDVAVGLFYFNVLLFFIFLVPWVLRWIMFKDNALADLKHPVLSAFYPTIAVSCLVLGADFINIGHNMFWGGVFWTLGAIGMFLFSLIVPFYMFKSESIKLDHVNPGWYIPPVGLIVIPIAGSLIMPHLTGVWHELTVLINYFGWGAGFFLYLALLAVVIYRFILHHPLPSAMAPTVWINLGPIGAGIVALINMVNNSPFITIKEPFYIFSFIFWGFGLWWSLMAIIMTLYYVKKLKLPYAMSWWAFIFPLGAYVASSHLVYKIFKFSIIDYIGFGLYWLLFFFWAITLIKTTNKVYTGEVFKGH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q57999</accession>
    <name>Y579_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0579</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0579</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0578.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64372">
        <property type="entry name" value="C64372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57999"/>
    <dbReference type="SMR" id="Q57999"/>
    <dbReference type="STRING" id="243232.MJ_0579"/>
    <dbReference type="EnsemblBacteria" id="AAB98570">
        <property type="protein sequence ID" value="AAB98570"/>
        <property type="gene ID" value="MJ_0579"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0579"/>
    <dbReference type="eggNOG" id="arCOG04073">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067767_0_0_2"/>
    <dbReference type="InParanoid" id="Q57999"/>
    <dbReference type="OMA" id="DVEEPNC"/>
    <dbReference type="PhylomeDB" id="Q57999"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106940" description="Uncharacterized protein MJ0579">
        <location>
            <begin position="1"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="130"/>
            <end position="137"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="269" mass="30192" checksum="B5BE21D15BFF7079" modified="1996-11-01" version="1">MSEMMIVAVTGGKGGTGKSTLSANLFFYFIENYKTALIDCDVETPNLPYLTGCEDLFLAREVFIEVPNIEEGKTYNYNNVCKEGALLKVGDKLIFIEDLCSGCKACGINSNITFKKKSIGKIYEKKFDNGYLIVGKSNLGERKTAKIVTETKKYGLSKNCEINIVDTAAGTHCNVVRALINADKVLIVTEPTPFGVSDAKRIIKVVEKLNIPYKIVLNRYGISDLKIGYNFKIPYDKRIVECYCKGESFLKYNDLRNYIEEIANWIIWG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58010</accession>
    <name>ACD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Acetate--CoA ligase [ADP-forming]</fullName>
            <ecNumber evidence="1">6.2.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">ADP-forming acetyl coenzyme A synthetase</fullName>
            <shortName evidence="2">ACS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ordered locus">MJ0590</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Bacteriol." volume="184" first="636" last="644">
            <title>Novel type of ADP-forming acetyl coenzyme A synthetase in hyperthermophilic archaea: heterologous expression and characterization of isoenzymes from the sulfate reducer Archaeoglobus fulgidus and the methanogen Methanococcus jannaschii.</title>
            <authorList>
                <person name="Musfeldt M."/>
                <person name="Schoenheit P."/>
            </authorList>
            <dbReference type="PubMed" id="11790732"/>
            <dbReference type="DOI" id="10.1128/JB.184.3.636-644.2002"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of acetate and ATP from acetyl-CoA by using ADP and phosphate. Can also use butyryl-CoA, but not phenylacetyl-CoA. Cannot catalyze the reverse reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">37 uM for acetyl-CoA</KM>
            <KM evidence="1">15 uM for ADP</KM>
            <KM evidence="1">470 uM for phosphate</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the acetate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the acetate CoA ligase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64373">
        <property type="entry name" value="F64373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870094.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58010"/>
    <dbReference type="SMR" id="Q58010"/>
    <dbReference type="STRING" id="243232.MJ_0590"/>
    <dbReference type="EnsemblBacteria" id="AAB98580">
        <property type="protein sequence ID" value="AAB98580"/>
        <property type="gene ID" value="MJ_0590"/>
    </dbReference>
    <dbReference type="GeneID" id="1451455"/>
    <dbReference type="KEGG" id="mja:MJ_0590"/>
    <dbReference type="eggNOG" id="arCOG01340">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007415_3_1_2"/>
    <dbReference type="InParanoid" id="Q58010"/>
    <dbReference type="OMA" id="SFMSQSG"/>
    <dbReference type="OrthoDB" id="2789at2157"/>
    <dbReference type="PhylomeDB" id="Q58010"/>
    <dbReference type="BRENDA" id="6.2.1.B11">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58010"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014089">
        <property type="entry name" value="AcCoA-synth-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02717">
        <property type="entry name" value="AcCoA-syn-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106948" description="Acetate--CoA ligase [ADP-forming]">
        <location>
            <begin position="1"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11790732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0000312" key="3">
        <source>
            <dbReference type="EMBL" id="AAB98580.1"/>
        </source>
    </evidence>
    <sequence length="704" mass="78172" checksum="A60E5F19DA1B2C58" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58073</accession>
    <name>Y659_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0659</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0659</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64382">
        <property type="entry name" value="C64382"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870164.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58073"/>
    <dbReference type="SMR" id="Q58073"/>
    <dbReference type="PRIDE" id="Q58073"/>
    <dbReference type="EnsemblBacteria" id="AAB98658">
        <property type="protein sequence ID" value="AAB98658"/>
        <property type="gene ID" value="MJ_0659"/>
    </dbReference>
    <dbReference type="GeneID" id="1451525"/>
    <dbReference type="KEGG" id="mja:MJ_0659"/>
    <dbReference type="eggNOG" id="arCOG09670">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850645_0_0_2"/>
    <dbReference type="OMA" id="IEKGYCD"/>
    <dbReference type="OrthoDB" id="110330at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106976" description="Uncharacterized protein MJ0659">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <sequence length="138" mass="15470" checksum="96A8AE06757D563C" modified="1996-11-01" version="1">MVSEIIKLIEEGKIEEVLKKVEEIKGDAQLEIIALTLIEKGYCDEAVKVAEKISSFGLKDEVLRKVAIAYIENGEIDKAMALVEKIKTETDLEKIAMKLIEIKKYREALKVAEKIKSRAIKEGILMAIINALLDELGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58084</accession>
    <name>Y670_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0670</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0670</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64383">
        <property type="entry name" value="F64383"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58084"/>
    <dbReference type="SMR" id="Q58084"/>
    <dbReference type="STRING" id="243232.MJ_0670"/>
    <dbReference type="EnsemblBacteria" id="AAB98664">
        <property type="protein sequence ID" value="AAB98664"/>
        <property type="gene ID" value="MJ_0670"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0670"/>
    <dbReference type="eggNOG" id="arCOG00120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052777_0_0_2"/>
    <dbReference type="InParanoid" id="Q58084"/>
    <dbReference type="OMA" id="ICYGLGY"/>
    <dbReference type="PhylomeDB" id="Q58084"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008471">
        <property type="entry name" value="MnmC-like_methylTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05430">
        <property type="entry name" value="Methyltransf_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106983" description="Uncharacterized protein MJ0670">
        <location>
            <begin position="1"/>
            <end position="356"/>
        </location>
    </feature>
    <sequence length="356" mass="41684" checksum="D7B8BA2E16A92E11" modified="1996-11-01" version="1">MRVMLPNKKALEIIRKYMKIYNGKNEKDIKERLIKELKEEHVLVETEDGTYTLKAEDEEEMMHSKVGALKEAIYKFAKPSKITDLSNPRVLDLCSGMGYNAIAALHYNKNAEIDMVEICEEVLFLTLFLDIPYKEHEIIKDKVREYFLNKIGIEYKSDYDNINLYVGDARKFIIKSDKKYNVVFHDAFSPKRDPTLYTYDFLKEIYKRMEDNGVLISYSSAIPFRSALVDCGFVISEKESVGRKRGITLAYKNPNFKPNRINEVDERVIALSVIALPYRDETLSLTKDKIIEDREERREKLKEKLIKIGKYLSTKQIKKGNIPEEILKIQKEDLNSSEIIKKMRLKFFKDANIFIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58090</accession>
    <name>PDXS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="1">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="1">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pdxS</name>
        <name type="ordered locus">MJ0677</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2007-05" db="PDB data bank">
            <title>Crystal structure of pyridoxine biosynthesis protein from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Manzoku M."/>
                <person name="Ebihara A."/>
                <person name="Chen L."/>
                <person name="Fu Z.-Q."/>
                <person name="Chrzas J."/>
                <person name="Wang B.-C."/>
                <person name="Yokoyama S."/>
                <person name="Kuramitsu S."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = H(+) + 3 H2O + L-glutamate + phosphate + pyridoxal 5'-phosphate</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64384">
        <property type="entry name" value="E64384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870182.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2YZR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C=1-330"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YZR"/>
    <dbReference type="AlphaFoldDB" id="Q58090"/>
    <dbReference type="SMR" id="Q58090"/>
    <dbReference type="STRING" id="243232.MJ_0677"/>
    <dbReference type="EnsemblBacteria" id="AAB98672">
        <property type="protein sequence ID" value="AAB98672"/>
        <property type="gene ID" value="MJ_0677"/>
    </dbReference>
    <dbReference type="GeneID" id="1451543"/>
    <dbReference type="KEGG" id="mja:MJ_0677"/>
    <dbReference type="eggNOG" id="arCOG04075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055352_1_0_2"/>
    <dbReference type="InParanoid" id="Q58090"/>
    <dbReference type="OMA" id="RYANRGW"/>
    <dbReference type="OrthoDB" id="64452at2157"/>
    <dbReference type="PhylomeDB" id="Q58090"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="EvolutionaryTrace" id="Q58090"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016843">
        <property type="term" value="F:amine-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:cellular amino acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033983">
        <property type="entry name" value="Thiazole_synthase_ThiG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PTHR31829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05690">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00343">
        <property type="entry name" value="TIGR00343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000109435" description="Pyridoxal 5'-phosphate synthase subunit PdxS">
        <location>
            <begin position="1"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="1">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="271"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="19"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="39"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="77"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="86"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="98"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="117"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="124"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="131"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="143"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="157"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="190"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="194"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="213"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="242"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="254"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="268"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="272"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="293"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="297"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2YZR"/>
        </source>
    </evidence>
    <sequence length="330" mass="35856" checksum="A4FCCE386CEF56AB" modified="1997-11-01" version="1">MKKGTDLLKKGFAKMVKHGVVMDVTNVEQAQIAEEAGAVAVMALERVPADIRAAGGVARMSDPALIEEIMDAVSIPVMAKCRIGHTTEALVLEAIGVDMIDESEVLTQADPFFHIYKKKFNVPFVCGARNLGEAVRRIWEGAAMIRTKGEAGTGNIVEAVRHMRLMNEAIAQLQRMTDEEVYGVAKFYANRYAELAKTVREGMGLPATVLENEPIYEGFTLAEIIDGLYEVLLEVKKLGRLPVVNFAAGGVATPADAALMMQLGSDGVFVGSGIFKSENPLERARAIVEATYNYDKPDIVAEVSKNLGEAMKGIDITQISEAEKMQYRGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58095</accession>
    <name>RTCB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="1">6.5.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">3'-phosphate/5'-hydroxy nucleic acid ligase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mja hyp2 intein</fullName>
                <ecNumber>3.1.-.-</ecNumber>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">rtcB</name>
        <name type="ordered locus">MJ0682</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + diphosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Ligation proceeds through 3 nucleotidyl transfer steps, with 2',3'-cyclic phosphate termini being hydrolyzed to 3'-P termini in a step that precedes 3'-P activation with GMP. In the first nucleotidyl transfer step, RtcB reacts with GTP to form a covalent RtcB-histidine-GMP intermediate with release of PPi; in the second step, the GMP moiety is transferred to the RNA 3'-P; in the third step, the 5'-OH from the opposite RNA strand attacks the activated 3'-P to form a 3',5'-phosphodiester bond and release GMP.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RtcB family.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.8" evidence="1"/>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64385">
        <property type="entry name" value="B64385"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870187.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58095"/>
    <dbReference type="SMR" id="Q58095"/>
    <dbReference type="STRING" id="243232.MJ_0682"/>
    <dbReference type="EnsemblBacteria" id="AAB98677">
        <property type="protein sequence ID" value="AAB98677"/>
        <property type="gene ID" value="MJ_0682"/>
    </dbReference>
    <dbReference type="GeneID" id="1451548"/>
    <dbReference type="KEGG" id="mja:MJ_0682"/>
    <dbReference type="eggNOG" id="arCOG03158">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012374_0_0_2"/>
    <dbReference type="InParanoid" id="Q58095"/>
    <dbReference type="OMA" id="VHNCGVR"/>
    <dbReference type="OrthoDB" id="22545at2157"/>
    <dbReference type="PhylomeDB" id="Q58095"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
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    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000036183" description="tRNA-splicing ligase RtcB, 1st part" evidence="2">
        <location>
            <begin position="1"/>
            <end position="97"/>
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    <feature type="chain" id="PRO_0000036184" description="Mja hyp2 intein" evidence="2">
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    <feature type="domain" description="DOD-type homing endonuclease" evidence="3">
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            <begin position="245"/>
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    </feature>
    <feature type="active site" description="GMP-histidine intermediate" evidence="1">
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            <name>GMP</name>
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            <position position="872"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
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    <sequence length="968" mass="110206" checksum="39A2A66280466130" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58109</accession>
    <name>HIS7_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="1">IGPD</shortName>
            <ecNumber evidence="1">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hisB</name>
        <name type="ordered locus">MJ0698</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="B64387"/>
    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAB98691">
        <property type="protein sequence ID" value="AAB98691"/>
        <property type="gene ID" value="MJ_0698"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0698"/>
    <dbReference type="eggNOG" id="arCOG04398">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044308_2_0_2"/>
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    <dbReference type="PhylomeDB" id="Q58109"/>
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        <property type="reaction ID" value="UER00011"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="HisB"/>
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    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
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    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
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        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
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        <property type="entry name" value="PTHR23133"/>
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        <property type="entry name" value="IGPD"/>
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    </dbReference>
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        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158189" description="Imidazoleglycerol-phosphate dehydratase">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <sequence length="197" mass="22158" checksum="B4E5500ADB4A5D01" modified="1996-11-01" version="1">MFGGNMRIFEVMRETKETNIYLKINIDGTGKYKIDTGIPFFDHLLASFAKHGCFDLIVKARGDLEIDDHHTVEDVGICLGLALNQIEKRNIFRFGWAIIPMDDARATVAIDLSGRSYCVGNYEPKREFVGDLATENINHFFESVASYGMLNIHYEVIGKNEHHKAEALFKAFGVALDLATKIDERKGVISTKGEVKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58124</accession>
    <name>Y714_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0714</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0714</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0310 and MJ1340.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64389">
        <property type="entry name" value="B64389"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58124"/>
    <dbReference type="SMR" id="Q58124"/>
    <dbReference type="STRING" id="243232.MJ_0714"/>
    <dbReference type="EnsemblBacteria" id="AAB98709">
        <property type="protein sequence ID" value="AAB98709"/>
        <property type="gene ID" value="MJ_0714"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0714"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118613_4_0_2"/>
    <dbReference type="InParanoid" id="Q58124"/>
    <dbReference type="OMA" id="FEMDSRA"/>
    <dbReference type="PhylomeDB" id="Q58124"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="PTHR13323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107000" description="Uncharacterized protein MJ0714">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="118" mass="12345" checksum="D6380D2D6E0CFD0E" modified="1996-11-01" version="1">MVESMIDRVLLELNRTDGVKGSMVVGKDGLVIASQLPGNVDAELVGAMASAAFGAAERTSSEIGLSGLEQTMIEGEHGKTLMVDAGEGILVVLTDAKVNLGLIRITMKRAADKIKAMF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58151</accession>
    <name>SOR_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative superoxide reductase</fullName>
            <shortName>SOR</shortName>
            <ecNumber>1.15.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0741</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Uses electrons from reduced NADP, by way of rubredoxin and an oxidoreductase, to catalyze the reduction of superoxide to hydrogen peroxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 H(+) + reduced [rubredoxin] + superoxide = H2O2 + oxidized [rubredoxin]</text>
            <dbReference type="Rhea" id="RHEA:21324"/>
            <dbReference type="Rhea" id="RHEA-COMP:10302"/>
            <dbReference type="Rhea" id="RHEA-COMP:10303"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18421"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.15.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the desulfoferrodoxin family.</text>
    </comment>
    <dbReference type="EC" id="1.15.1.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64392">
        <property type="entry name" value="E64392"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870246.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58151"/>
    <dbReference type="SMR" id="Q58151"/>
    <dbReference type="STRING" id="243232.MJ_0741"/>
    <dbReference type="EnsemblBacteria" id="AAB98735">
        <property type="protein sequence ID" value="AAB98735"/>
        <property type="gene ID" value="MJ_0741"/>
    </dbReference>
    <dbReference type="GeneID" id="1451618"/>
    <dbReference type="KEGG" id="mja:MJ_0741"/>
    <dbReference type="eggNOG" id="arCOG02146">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118960_2_1_2"/>
    <dbReference type="InParanoid" id="Q58151"/>
    <dbReference type="OMA" id="FEKKHTP"/>
    <dbReference type="OrthoDB" id="106274at2157"/>
    <dbReference type="PhylomeDB" id="Q58151"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050605">
        <property type="term" value="F:superoxide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002742">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036073">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01880">
        <property type="entry name" value="Desulfoferrodox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49367">
        <property type="entry name" value="SSF49367"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00332">
        <property type="entry name" value="neela_ferrous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000140871" description="Putative superoxide reductase">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P82385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="116" mass="13950" checksum="4CF2C76237DE0673" modified="1996-11-01" version="1">MTHYCGINRMKEGTDFEKKHTPFIECKDRVKANDYFEVKISTGIPHPMEDNHFIHWIELYMGDLYLARVDFTQFMKPEVKLMVKAPSKEHEKFILRALMRCNLHGVWEYEKEILLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58175</accession>
    <name>HCP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydroxylamine reductase</fullName>
            <ecNumber evidence="1">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Hybrid-cluster protein</fullName>
            <shortName evidence="1">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hcp</name>
        <name type="ordered locus">MJ0765</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>A + H2O + NH4(+) = AH2 + H(+) + hydroxylamine</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="1">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HCP family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98761" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98761.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64395">
        <property type="entry name" value="E64395"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58175"/>
    <dbReference type="SMR" id="Q58175"/>
    <dbReference type="STRING" id="243232.MJ_0765"/>
    <dbReference type="PRIDE" id="Q58175"/>
    <dbReference type="EnsemblBacteria" id="AAB98761">
        <property type="protein sequence ID" value="AAB98761"/>
        <property type="gene ID" value="MJ_0765"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0765"/>
    <dbReference type="eggNOG" id="arCOG02430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038344_2_0_2"/>
    <dbReference type="InParanoid" id="Q58175"/>
    <dbReference type="OMA" id="CAYAQGM"/>
    <dbReference type="PhylomeDB" id="Q58175"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01914">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
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        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="PTHR30109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="PTHR30109:SF0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000076">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151691" description="Hydroxylamine reductase">
        <location>
            <begin position="1"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="1">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="484"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="1">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="539" mass="60458" checksum="46442DD4FCBCE5A5" modified="2000-05-30" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58188</accession>
    <name>Y778_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0778</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0778</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64397">
        <property type="entry name" value="B64397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870283.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58188"/>
    <dbReference type="SMR" id="Q58188"/>
    <dbReference type="STRING" id="243232.MJ_0778"/>
    <dbReference type="DNASU" id="1451655"/>
    <dbReference type="EnsemblBacteria" id="AAB98768">
        <property type="protein sequence ID" value="AAB98768"/>
        <property type="gene ID" value="MJ_0778"/>
    </dbReference>
    <dbReference type="GeneID" id="1451655"/>
    <dbReference type="KEGG" id="mja:MJ_0778"/>
    <dbReference type="eggNOG" id="arCOG01858">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073842_1_0_2"/>
    <dbReference type="InParanoid" id="Q58188"/>
    <dbReference type="OMA" id="GRSKTHG"/>
    <dbReference type="OrthoDB" id="88300at2157"/>
    <dbReference type="PhylomeDB" id="Q58188"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004454">
        <property type="entry name" value="HD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00295">
        <property type="entry name" value="TIGR00295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107029" description="Uncharacterized protein MJ0778">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="18"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <sequence length="169" mass="18940" checksum="912EC4EAB768C8F2" modified="1996-11-01" version="1">MEFEKALSILKNLCSENVVEHCLAVSEYAYELALAIKNKGYEVDVELVRLGGLLHDIGRSRTHGIEHGVVGAEILRELGFDEKLALIAERHIGAGITKEEAIELGLPPKDYLPITLEEKIVAHADNLIFGTKRVEIDDVIKKFEKRLGKNHPSIKRIILLNDEINNLLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58236</accession>
    <name>Y826_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0826</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0826</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64403">
        <property type="entry name" value="B64403"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870337.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58236"/>
    <dbReference type="SMR" id="Q58236"/>
    <dbReference type="STRING" id="243232.MJ_0826"/>
    <dbReference type="EnsemblBacteria" id="AAB98825">
        <property type="protein sequence ID" value="AAB98825"/>
        <property type="gene ID" value="MJ_0826"/>
    </dbReference>
    <dbReference type="GeneID" id="1451709"/>
    <dbReference type="KEGG" id="mja:MJ_0826"/>
    <dbReference type="eggNOG" id="arCOG01912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116732_3_0_2"/>
    <dbReference type="InParanoid" id="Q58236"/>
    <dbReference type="OMA" id="LYFPAWG"/>
    <dbReference type="OrthoDB" id="99396at2157"/>
    <dbReference type="PhylomeDB" id="Q58236"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107064" description="Uncharacterized protein MJ0826">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="138" mass="15094" checksum="24B8EA4E23E20534" modified="1996-11-01" version="1">MEIGYIFILAGFLVIALEAIVPGLYFPAWGIALLIYGVVLLIIPQYAFISAIIAGVLTIIILHKFVYGVGKEIKVGAERFVGMIGIAIEDFEENGYGRIKIENQIWLAKSKDKIKNGDKVEIVGVEGVSLIVKKVEGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q58252</accession>
    <name>MCRB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase I subunit beta</fullName>
            <shortName>MCR I beta</shortName>
            <ecNumber evidence="1">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme-B sulfoethylthiotransferase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mcrB</name>
        <name type="ordered locus">MJ0842</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="1">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-coenzyme M reductase beta subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58252"/>
    <dbReference type="SMR" id="Q58252"/>
    <dbReference type="STRING" id="243232.MJ_0842"/>
    <dbReference type="EnsemblBacteria" id="AAB98847">
        <property type="protein sequence ID" value="AAB98847"/>
        <property type="gene ID" value="MJ_0842"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0842"/>
    <dbReference type="eggNOG" id="arCOG04860">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_617682_0_0_2"/>
    <dbReference type="InParanoid" id="Q58252"/>
    <dbReference type="OMA" id="TAMFEMG"/>
    <dbReference type="PhylomeDB" id="Q58252"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003179">
        <property type="entry name" value="Me_CoM_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022679">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022680">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02241">
        <property type="entry name" value="MCR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02783">
        <property type="entry name" value="MCR_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000263">
        <property type="entry name" value="Meth_CoM_rd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03257">
        <property type="entry name" value="met_CoM_red_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147465" description="Methyl-coenzyme M reductase I subunit beta">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="373"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P11560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="447" mass="47789" checksum="52C76BB2C4C425E7" modified="1998-12-15" version="2">MIPMVKYKDTINLYDEKGKLVEENVPLEAISPLHNPTIQKLVKDVKRTVAVNLAGIENALRTGQVGGKGCMIKGRELDLPIVENAETIAEYVEKVVRVSEDDDTSIKLINDGKQMAVQIPSKRLDVAAEYSVSVLVTAQALKEAIIRTFDVDIFDAPMVHAAVLGGYPHEVTMKGSNIAALLGSPLSLEGPGYALRNIMANHFVACTKKNVMNAVAFAAIMEQTAMFEMGDAVGLFERLHLLGLAYQGLNADNLVIDLVKANGKNGTVGTVVASVVERALEDGVIKEDKTLPSGFKLYKPVDVAKWNAYAAAGLVAAAIVNCGAARAAQNIASTILYYNDILEYETGLPGVDFGRAEGTAVGFSFFSHSIYGGGGPGIFNGNHIVTRHSKGFAIPPVAAAMCLDAGTQMFSPERTSALVGTVFSAIDEFREPLKYVIKGALEVKDKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58268</accession>
    <name>Y858_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0858</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0858</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64407">
        <property type="entry name" value="B64407"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870373.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58268"/>
    <dbReference type="SMR" id="Q58268"/>
    <dbReference type="STRING" id="243232.MJ_0858"/>
    <dbReference type="PRIDE" id="Q58268"/>
    <dbReference type="EnsemblBacteria" id="AAB98864">
        <property type="protein sequence ID" value="AAB98864"/>
        <property type="gene ID" value="MJ_0858"/>
    </dbReference>
    <dbReference type="GeneID" id="1451747"/>
    <dbReference type="KEGG" id="mja:MJ_0858"/>
    <dbReference type="eggNOG" id="arCOG04905">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_160441_0_0_2"/>
    <dbReference type="OMA" id="QAYFVDP"/>
    <dbReference type="OrthoDB" id="113752at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008032">
        <property type="entry name" value="DUF749"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035933">
        <property type="entry name" value="MTH1880"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016458">
        <property type="entry name" value="UCP005648_Ca-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05370">
        <property type="entry name" value="DUF749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005648">
        <property type="entry name" value="UCP005648_Ca-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75412">
        <property type="entry name" value="SSF75412"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107083" description="Uncharacterized protein MJ0858">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <sequence length="113" mass="13335" checksum="D2FE8B783DBCB0AB" modified="1996-11-01" version="1">MNDKNVEFVATLISILTVKEALNSEMENFVKVRAAIDKRELKDDDKVAIFNINSTTSYQVFFIDKDTNIEELKEEFKKMNVRINYDSEQVLKRYIERLRIQNNSKPISNNNKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q58295</accession>
    <name>DPOL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase</fullName>
            <ecNumber>2.7.7.7</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Mja pol-1 intein</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Mja pol-2 intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">pol</name>
        <name type="ordered locus">MJ0885</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="PTM">
        <text evidence="3">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64410">
        <property type="entry name" value="E64410"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58295"/>
    <dbReference type="SMR" id="Q58295"/>
    <dbReference type="STRING" id="243232.MJ_0885"/>
    <dbReference type="PRIDE" id="Q58295"/>
    <dbReference type="EnsemblBacteria" id="AAB98889">
        <property type="protein sequence ID" value="AAB98889"/>
        <property type="gene ID" value="MJ_0885"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0885"/>
    <dbReference type="eggNOG" id="arCOG00328">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG00329">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000203_6_4_2"/>
    <dbReference type="InParanoid" id="Q58295"/>
    <dbReference type="OMA" id="YAHNSYY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041005">
        <property type="entry name" value="PI-TkoII_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18714">
        <property type="entry name" value="PI-TkoII_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="SSF53098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="SSF56672"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000007317" description="DNA polymerase, 1st part" evidence="1">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000007318" description="Mja pol-1 intein" evidence="1">
        <location>
            <begin position="426"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000007319" description="DNA polymerase, 2nd part" evidence="1">
        <location>
            <begin position="795"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000007320" description="Mja pol-2 intein" evidence="1">
        <location>
            <begin position="883"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000007321" description="DNA polymerase, 3rd part" evidence="1">
        <location>
            <begin position="1359"/>
            <end position="1634"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease 1" evidence="2">
        <location>
            <begin position="552"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease 2" evidence="2">
        <location>
            <begin position="1163"/>
            <end position="1295"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1634" mass="191710" checksum="84A1FAFAB1F97DDD" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58300</accession>
    <name>Y890_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0148 protein MJ0890</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0890</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0148 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64411">
        <property type="entry name" value="B64411"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58300"/>
    <dbReference type="SMR" id="Q58300"/>
    <dbReference type="STRING" id="243232.MJ_0890"/>
    <dbReference type="EnsemblBacteria" id="AAB98906">
        <property type="protein sequence ID" value="AAB98906"/>
        <property type="gene ID" value="MJ_0890"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0890"/>
    <dbReference type="eggNOG" id="arCOG00578">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142653_2_0_2"/>
    <dbReference type="InParanoid" id="Q58300"/>
    <dbReference type="OMA" id="AKMLGKH"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00343">
        <property type="entry name" value="UPF0148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022954">
        <property type="entry name" value="UPF0148"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159857" description="UPF0148 protein MJ0890">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="137" mass="15914" checksum="B0EEAC8A4245C0C8" modified="1996-11-01" version="1">MIKTVIDNLCYNFGENMKNDDAIKVLSNELLKGAKMLSTHCSKCGCPLFEKDGKIYCPICEKLKNKETIEKGENEKEIKNEIERKKSEINEILDLNKVVMDKINYLVMKLKEEDEVSRIREIAEAIYVLIKLKKKIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58308</accession>
    <name>FLAG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative flagella-related protein G</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaG</name>
        <name type="ordered locus">MJ0898</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal FlaG family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64412">
        <property type="entry name" value="B64412"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58308"/>
    <dbReference type="STRING" id="243232.MJ_0898"/>
    <dbReference type="EnsemblBacteria" id="AAB98901">
        <property type="protein sequence ID" value="AAB98901"/>
        <property type="gene ID" value="MJ_0898"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0898"/>
    <dbReference type="eggNOG" id="arCOG01822">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134827_1_0_2"/>
    <dbReference type="InParanoid" id="Q58308"/>
    <dbReference type="OMA" id="MINGEIM"/>
    <dbReference type="PhylomeDB" id="Q58308"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200">
        <property type="entry name" value="PTHR42200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Arch_flagellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000087273" description="Putative flagella-related protein G">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="154" mass="16537" checksum="67C5C67B3C7885BC" modified="1996-11-01" version="1">MIYLASSAMSEIVMFVAVLLIAAFVAGILTTSTYKISLNINKKGDALATKLSQDFEIINDPGDIVRNSSAGTIALYIKNTGKDPIIFTNDSFTVIIDGSIVEINTTNQLTSPGSNILSPGDVGEIVVNYNETGYHRIKVISECGISRIIRGYIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58312</accession>
    <name>FLAK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Preflagellin peptidase</fullName>
            <shortName>PFP</shortName>
            <ecNumber>3.4.23.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaK</name>
        <name type="ordered locus">MJ0902</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves the N-terminal leader peptide from preflagellins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Cleaves the signal peptide of 3 to 12 amino acids from the N-terminal of preflagellin, usually at Arg-Gly-|- or Lys-Gly-|-, to release flagellin.</text>
            <dbReference type="EC" id="3.4.23.52"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase A24 family. Archaeal preflagellin peptidase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98907" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.4.23.52"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98907.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64412">
        <property type="entry name" value="F64412"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496655.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58312"/>
    <dbReference type="SMR" id="Q58312"/>
    <dbReference type="STRING" id="243232.MJ_0902"/>
    <dbReference type="MEROPS" id="A24.016"/>
    <dbReference type="EnsemblBacteria" id="AAB98907">
        <property type="protein sequence ID" value="AAB98907"/>
        <property type="gene ID" value="MJ_0902"/>
    </dbReference>
    <dbReference type="GeneID" id="1451791"/>
    <dbReference type="KEGG" id="mja:MJ_0902"/>
    <dbReference type="eggNOG" id="arCOG02298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1197648_0_0_2"/>
    <dbReference type="InParanoid" id="Q58312"/>
    <dbReference type="OrthoDB" id="87374at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009655">
        <property type="entry name" value="Preflagellin_peptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06847">
        <property type="entry name" value="Arc_PepC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1209">Archaeal flagellum biogenesis</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107094" description="Preflagellin peptidase">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="19"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="73"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="1">
        <location>
            <begin position="140"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="1">
        <location>
            <begin position="218"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="site" description="Essential for catalysis" evidence="1">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="site" description="Essential for catalysis" evidence="1">
        <location>
            <position position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="234" mass="26294" checksum="A0F94DDCA7F7D2D4" modified="2012-10-03" version="2">MINFIVGAIGLLIASIYDLKSREIEDYVWVSMVIFGLIYNGYLSFISHDMLYVIQSIVGFIVCFFLGFFMFLLGVGGGDGKLIMGLGALIPKYNMPIHTPLGAILNYLYLPSFPIMVVINAMFFSITLPIIIFLRNVIRGVKPKTKKEVLCMFLGEKMKVSEAIKKERLILGNHENLKLLPSAEKDCDFSKFDKNEEIWVTPAIPFVVPIFLSYLLTSIIGDKIIGIFLSVFGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58321</accession>
    <name>Y911_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Magnesium-chelatase subunit ChlI homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0911</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64413">
        <property type="entry name" value="G64413"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58321"/>
    <dbReference type="SMR" id="Q58321"/>
    <dbReference type="STRING" id="243232.MJ_0911"/>
    <dbReference type="PRIDE" id="Q58321"/>
    <dbReference type="EnsemblBacteria" id="AAB98913">
        <property type="protein sequence ID" value="AAB98913"/>
        <property type="gene ID" value="MJ_0911"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0911"/>
    <dbReference type="eggNOG" id="arCOG00438">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016684_0_2_2"/>
    <dbReference type="InParanoid" id="Q58321"/>
    <dbReference type="OMA" id="RISAVCG"/>
    <dbReference type="PhylomeDB" id="Q58321"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="PTHR32039"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000206875" description="Magnesium-chelatase subunit ChlI homolog">
        <location>
            <begin position="1"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="340"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="343"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="365" mass="41677" checksum="CB0046955E1881E3" modified="1996-11-01" version="1">MMIMQYIYPFTAIVGQEKMKKALILNAINPKIGGVLIRGEKGTAKSTAVRALADLLPEIEIVEGCPFNCDPNGNLCDICKEKKKRGELKTTKKKMKVVNLPIGATEDRVIGTLDIEKAIKEGIKALEPGILAEANRNILYIDEVNLLDDHIIDVLLDAAAMGWNIIEREGVKIKHPSRFILVGTMNPEEGELRPQILDRFGLMVDVEGLNDVKDRVEVIKRVEEFNENPEAFYKKFEEEQNKLRERIIKARELLNKVEISDDLLEFISKVCIELGIQTNRADITVVRTAKALAAYNGRTYVTIDDVKEAMELALPHRMRRKPFEPPQLNKEKLEQMINEFKQQNNKDNEEKEEHKDDDVKKNMMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58339</accession>
    <name>PUR8_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Adenylosuccinate lyase</fullName>
            <shortName>ASL</shortName>
            <ecNumber evidence="2">4.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Adenylosuccinase</fullName>
            <shortName>ASase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">purB</name>
        <name type="ordered locus">MJ0929</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes two reactions in de novo purine nucleotide biosynthesis. Catalyzes the breakdown of 5-aminoimidazole- (N-succinylocarboxamide) ribotide (SAICAR or 2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate) to 5-aminoimidazole-4-carboxamide ribotide (AICAR or 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) and fumarate, and of adenylosuccinate (ADS or N(6)-(1,2-dicarboxyethyl)-AMP) to adenosine monophosphate (AMP) and fumarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>N(6)-(1,2-dicarboxyethyl)-AMP = AMP + fumarate</text>
            <dbReference type="Rhea" id="RHEA:16853"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:16854"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + fumarate</text>
            <dbReference type="Rhea" id="RHEA:23920"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="EC" id="4.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:23921"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer. Residues from neighboring subunits contribute catalytic and substrate-binding residues to each active site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.2" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64416">
        <property type="entry name" value="A64416"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58339"/>
    <dbReference type="SMR" id="Q58339"/>
    <dbReference type="STRING" id="243232.MJ_0929"/>
    <dbReference type="EnsemblBacteria" id="AAB98931">
        <property type="protein sequence ID" value="AAB98931"/>
        <property type="gene ID" value="MJ_0929"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0929"/>
    <dbReference type="eggNOG" id="arCOG01747">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030949_0_1_2"/>
    <dbReference type="InParanoid" id="Q58339"/>
    <dbReference type="OMA" id="ASSCEKI"/>
    <dbReference type="PhylomeDB" id="Q58339"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-18798"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00132"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00075">
        <property type="reaction ID" value="UER00336"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070626">
        <property type="term" value="F:(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004018">
        <property type="term" value="F:N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044208">
        <property type="term" value="P:'de novo' AMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019468">
        <property type="entry name" value="AdenyloSucc_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004769">
        <property type="entry name" value="Pur_lyase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10397">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00998">
        <property type="entry name" value="ADSL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00928">
        <property type="entry name" value="purB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137888" description="Adenylosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="2">
        <location>
            <position position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="21"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>N(6)-(1,2-dicarboxyethyl)-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="87"/>
            <end position="89"/>
        </location>
        <ligand>
            <name>N(6)-(1,2-dicarboxyethyl)-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="114"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>N(6)-(1,2-dicarboxyethyl)-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>N(6)-(1,2-dicarboxyethyl)-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>N(6)-(1,2-dicarboxyethyl)-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="293"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>N(6)-(1,2-dicarboxyethyl)-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="332"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>N(6)-(1,2-dicarboxyethyl)-AMP</name>
            <dbReference type="ChEBI" id="CHEBI:57567"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P0AB89"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="462" mass="52592" checksum="D0E89A0D2F0C1894" modified="1996-11-01" version="1">MELNQIIKSGEKMAVHPIDYRYGTPEMRKVWEEENKLEKMLKVEAALAKAQAELGLIPKEAAEEINKKASTKYVKLERVKEIEKQTKHDVVAMIRALAEVCEGNAGEYIHFGATSNDIVDTANSLLIKESIEIIEDKLKQLRDILLDKAEEHKYTVCVGRTHGQHAIPTTYGMRFALWAAEIDRHLERLKEAKKRICVSMITGAVGTMAAMGEKGLEVHKRVAEILGLEPVLISNQVIQRDRHAEFVFLLALIAQTLNKIGVTVRSMQRTEIGELEEEFDPTKQTGSSTMPHKRNPITFEQICGLSRVIKSLCIAEMDNIPLWEERDLTNSSAERCIFAEVCVLTDHILTLAIKGVKKLKVNKENVERNLELTKGLIMAERIMIELAKRGMGRQTAHEIVRQCAMKAYEEKRHLKDILLENEEVMKYITKEELEELMNPKTYIGLAPQIVDEVIKTLKNKKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58343</accession>
    <name>Y933_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized membrane protein MJ0933</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0933</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64416">
        <property type="entry name" value="E64416"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58343"/>
    <dbReference type="STRING" id="243232.MJ_0933"/>
    <dbReference type="DNASU" id="1451823"/>
    <dbReference type="EnsemblBacteria" id="AAB98935">
        <property type="protein sequence ID" value="AAB98935"/>
        <property type="gene ID" value="MJ_0933"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0933"/>
    <dbReference type="eggNOG" id="arCOG02245">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036918_2_2_2"/>
    <dbReference type="InParanoid" id="Q58343"/>
    <dbReference type="OMA" id="AYGKRTF"/>
    <dbReference type="PhylomeDB" id="Q58343"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="PTHR13353"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00297">
        <property type="entry name" value="TIGR00297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107111" description="Uncharacterized membrane protein MJ0933">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="160"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="238" mass="25514" checksum="65BABA7106CE4780" modified="1996-11-01" version="1">MEVDTMETLIRLFVSILIICVLALMIKKSRCLDNEGVIGSSIMGFILLYFCGFKYLILLLSFFILGVLVSRVGLEKKKAKKMDETCRSLKNVLANGLIPILFAILAIFGFNWALIGYISSIAAATSDTFSSELGILSNEKPRLITTFEVVEKGTDGAITIFGTLAGVLGAFLIGLFGYLLFGDIKIVLCGTAGGIAGNLADSLVGALFERKGILNNEHVNLIATIVGGIIGVLIYCTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58361</accession>
    <name>Y951_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0951</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0951</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TmcAL family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64418">
        <property type="entry name" value="G64418"/>
    </dbReference>
    <dbReference type="PDB" id="3GMI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.91 A"/>
        <property type="chains" value="A=1-355"/>
    </dbReference>
    <dbReference type="PDBsum" id="3GMI"/>
    <dbReference type="AlphaFoldDB" id="Q58361"/>
    <dbReference type="SMR" id="Q58361"/>
    <dbReference type="STRING" id="243232.MJ_0951"/>
    <dbReference type="DNASU" id="1451848"/>
    <dbReference type="EnsemblBacteria" id="AAB98952">
        <property type="protein sequence ID" value="AAB98952"/>
        <property type="gene ID" value="MJ_0951"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0951"/>
    <dbReference type="eggNOG" id="arCOG03224">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777585_0_0_2"/>
    <dbReference type="InParanoid" id="Q58361"/>
    <dbReference type="OMA" id="MGSGQYM"/>
    <dbReference type="PhylomeDB" id="Q58361"/>
    <dbReference type="EvolutionaryTrace" id="Q58361"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032266">
        <property type="entry name" value="HIGH_NTase1_ass"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008513">
        <property type="entry name" value="tRNA(Met)_cyd_acetate_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37825">
        <property type="entry name" value="PTHR37825"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05636">
        <property type="entry name" value="HIGH_NTase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16581">
        <property type="entry name" value="HIGH_NTase1_ass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147199" description="Uncharacterized protein MJ0951">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
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            <begin position="30"/>
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    <feature type="strand" evidence="2">
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            <begin position="52"/>
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    <feature type="helix" evidence="2">
        <location>
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    <feature type="strand" evidence="2">
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            <begin position="76"/>
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    <feature type="strand" evidence="2">
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    <feature type="helix" evidence="2">
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    <feature type="strand" evidence="2">
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    <feature type="helix" evidence="2">
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            <begin position="126"/>
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    <feature type="strand" evidence="2">
        <location>
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    <feature type="helix" evidence="2">
        <location>
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    <feature type="strand" evidence="2">
        <location>
            <begin position="168"/>
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    <feature type="strand" evidence="2">
        <location>
            <begin position="174"/>
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    <feature type="turn" evidence="2">
        <location>
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        <location>
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        </location>
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        </location>
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    <feature type="helix" evidence="2">
        <location>
            <begin position="193"/>
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        </location>
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    <feature type="strand" evidence="2">
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    <feature type="helix" evidence="2">
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    <feature type="helix" evidence="2">
        <location>
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        </location>
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    <feature type="helix" evidence="2">
        <location>
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            <end position="245"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="248"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
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    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="272"/>
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        </location>
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    <feature type="helix" evidence="2">
        <location>
            <begin position="284"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="290"/>
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    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="305"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="318"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="350"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3GMI"/>
        </source>
    </evidence>
    <sequence length="355" mass="41322" checksum="2F683FE81FFE22FA" modified="1996-11-01" version="1">MLIGEIMDLNLKNFLEDREEIIRDAKRKDEKSFKDFKKIVEEIKERENKDKIVCDFTEYNPLHKGHKYALEKGKEHGIFISVLPGPLERSGRGIPYFLNRYIRAEMAIRAGADIVVEGPPMGIMGSGQYMRCLIKMFYSLGAEIIPRGYIPEKTMEKVIDCINKGYHIQVKPYKIICIETGEILGEKLNIDNYVIASMSQMIYKLNREGLKFNPKFVFVKRLEGISGTKIREAIFSGKFEDIKNMLPKTTLSILKELYDNGKLNELILKRFEDRILETANEYDLYEYLPSNVAEILEKKRPFNNIEEIKNSLPYGFSRHFRERILSKLEARIPNETLSKYINNYPAKIKILAVKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58375</accession>
    <name>SUMT_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uroporphyrinogen-III C-methyltransferase</fullName>
            <shortName>Urogen III methylase</shortName>
            <ecNumber evidence="2">2.1.1.107</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase</fullName>
            <shortName>SUMT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Uroporphyrinogen III methylase</fullName>
            <shortName>UROM</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cobA</name>
        <name type="synonym">corA</name>
        <name type="ordered locus">MJ0965</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of both cobalamin (vitamin B12) and coenzyme F430.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) + precorrin-2 + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:32459"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:32460"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98967" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98967.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64420">
        <property type="entry name" value="E64420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496681.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58375"/>
    <dbReference type="SMR" id="Q58375"/>
    <dbReference type="STRING" id="243232.MJ_0965"/>
    <dbReference type="EnsemblBacteria" id="AAB98967">
        <property type="protein sequence ID" value="AAB98967"/>
        <property type="gene ID" value="MJ_0965"/>
    </dbReference>
    <dbReference type="GeneID" id="1451863"/>
    <dbReference type="KEGG" id="mja:MJ_0965"/>
    <dbReference type="eggNOG" id="arCOG00644">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011276_7_0_2"/>
    <dbReference type="InParanoid" id="Q58375"/>
    <dbReference type="OMA" id="TRPEQEE"/>
    <dbReference type="OrthoDB" id="67633at2157"/>
    <dbReference type="PhylomeDB" id="Q58375"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00211"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="SSF53790"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000150371" description="Uroporphyrinogen-III C-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="118"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-homocysteine</name>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P21631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P29564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="242" mass="26340" checksum="BBE8388F036B6851" modified="2004-01-16" version="2">MTGKVILVGAGPGDPELITIKGLKAIKEADVVVYDDLISKELLNYAKKDAELIYVGKRKGKHSFKQEEINKILVEKAKEGKLVVRLKGGDPFVFGRGGEEILELKKHNIPYEVIPGITSAIAVPEVAGIPVTHRKVATSFTVVTGHEAEDKKEKQVDLSKLNADTIVILMGITNLENLVKELLQNPKRSKETPVAIIMEGTTKNQRVIKGTLGDIVEKAKKENARPPGVIVVGEVVNVLDSQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q58446</accession>
    <name>RPO1C_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo1C</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit A''</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mja rpo1C intein</fullName>
            </recommendedName>
            <alternativeName>
                <fullName>Mja rpoA'' intein</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Mja rpoA2 intein</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo1C</name>
        <name evidence="1" type="synonym">rpoA2</name>
        <name type="ordered locus">MJ1043</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms part of the jaw domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="2">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
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    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
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</entry>
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    <accession>Q58452</accession>
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            <fullName>UPF0045 protein MJ1052</fullName>
        </recommendedName>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the UPF0045 family.</text>
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        <location>
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    </feature>
    <feature type="strand" evidence="2">
        <location>
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    </feature>
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        <location>
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    </feature>
    <evidence type="ECO:0000305" key="1"/>
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        <source>
            <dbReference type="PDB" id="2EPI"/>
        </source>
    </evidence>
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</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58453</accession>
    <name>Y1053_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ1053</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1053</name>
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    <organism>
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        <lineage>
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        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
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                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
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            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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    <dbReference type="EnsemblBacteria" id="AAB99060">
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        <property type="gene ID" value="MJ_1053"/>
    </dbReference>
    <dbReference type="GeneID" id="1451950"/>
    <dbReference type="KEGG" id="mja:MJ_1053"/>
    <dbReference type="eggNOG" id="arCOG00921">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109226_0_0_2"/>
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    <dbReference type="OMA" id="TRVTWVD"/>
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        <property type="entry name" value="CHP03879_regulat_dom_put"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107153" description="Uncharacterized protein MJ1053">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <sequence length="163" mass="18789" checksum="F34029258C08497D" modified="1996-11-01" version="1">MIPLVPTSKTEIDKLEHVLILGTLFRPEILELIKDPIEKVTWVDSLAIAAGALAREKAGYTIREIADELGRTEQTIRKHLKGETKAGKLVRETYEMMKRGELNIEEVEKFLEAVVRKEELEKITDIKKLEEEIEKLKKENEELAAKLEKVKEKLKEVLSELEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58481</accession>
    <name>Y1081_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0047 protein MJ1081</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1081</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64434">
        <property type="entry name" value="H64434"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58481"/>
    <dbReference type="SMR" id="Q58481"/>
    <dbReference type="STRING" id="243232.MJ_1081"/>
    <dbReference type="EnsemblBacteria" id="AAB99083">
        <property type="protein sequence ID" value="AAB99083"/>
        <property type="gene ID" value="MJ_1081"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1081"/>
    <dbReference type="eggNOG" id="arCOG04214">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096980_1_1_2"/>
    <dbReference type="InParanoid" id="Q58481"/>
    <dbReference type="OMA" id="VRHTSCS"/>
    <dbReference type="PhylomeDB" id="Q58481"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="PTHR30615"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="SSF111038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01314">
        <property type="entry name" value="UPF0047"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000088525" description="UPF0047 protein MJ1081">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="138" mass="15613" checksum="F7D702871A9A3665" modified="1996-11-01" version="1">MLVIKMLFKYQIKTNKREELVDITPYIISAISESKVKDGIAVIYVPHTTAGITINENADPSVKHDIINFLSHLIPKNWNFTHLEGNSDAHIKSSLVGCSQTIIIKDGKPLLGTWQGIFFAEFDGPRRREFYVKIIGDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58490</accession>
    <name>CBIN_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobalt transport protein CbiN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Energy-coupling factor transporter probable substrate-capture protein CbiN</fullName>
            <shortName evidence="1">ECF transporter S component CbiN</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiN</name>
        <name type="ordered locus">MJ1090</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiN family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64436">
        <property type="entry name" value="A64436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870602.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58490"/>
    <dbReference type="STRING" id="243232.MJ_1090"/>
    <dbReference type="EnsemblBacteria" id="AAB99091">
        <property type="protein sequence ID" value="AAB99091"/>
        <property type="gene ID" value="MJ_1090"/>
    </dbReference>
    <dbReference type="GeneID" id="1451986"/>
    <dbReference type="KEGG" id="mja:MJ_1090"/>
    <dbReference type="eggNOG" id="arCOG04384">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_136197_2_0_2"/>
    <dbReference type="InParanoid" id="Q58490"/>
    <dbReference type="OMA" id="TEIEPDY"/>
    <dbReference type="OrthoDB" id="111394at2157"/>
    <dbReference type="PhylomeDB" id="Q58490"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00330">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003705">
        <property type="entry name" value="CbiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38662">
        <property type="entry name" value="PTHR38662"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02553">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01165">
        <property type="entry name" value="cbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0171">Cobalt transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000134702" description="Cobalt transport protein CbiN">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00330"/>
        </source>
    </evidence>
    <sequence length="95" mass="10428" checksum="FE9CFCE9EE6A62A9" modified="1996-11-01" version="1">METKHIILLAIVAIIIALPLIIYAGKGEEEGYFGGSDDQGCEVVEELGYKPWFHPIWEPPSGEIESLLFALQAAIGAIIIGYYIGYYNAKRQVAA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-18" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58517</accession>
    <name>COBY_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Adenosylcobinamide-phosphate guanylyltransferase</fullName>
            <shortName>AdoCbi-P guanylyltransferase</shortName>
            <ecNumber>2.7.7.62</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cobY</name>
        <name type="ordered locus">MJ1117</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Biochemistry" volume="47" first="3033" last="3037">
            <title>Identification and characterization of the 2-phospho-L-lactate guanylyltransferase involved in coenzyme F420 biosynthesis.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="18260642"/>
            <dbReference type="DOI" id="10.1021/bi702475t"/>
        </citation>
        <scope>FUNCTION AS A NUCLEOTIDYLTRANSFERASE</scope>
        <scope>KINETIC PARAMETERS</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Biochemistry" volume="48" first="5882" last="5889">
            <title>Biochemical characterization of the GTP:adenosylcobinamide-phosphate guanylyltransferase (CobY) enzyme of the hyperthermophilic archaeon Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Otte M.M."/>
                <person name="Escalante-Semerena J.C."/>
            </authorList>
            <dbReference type="PubMed" id="19489548"/>
            <dbReference type="DOI" id="10.1021/bi8023114"/>
        </citation>
        <scope>FUNCTION IN ADENOSYLCOBALAMIN BIOSYNTHESIS</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>GTP-BINDING</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>SUBUNIT</scope>
        <scope>ORDER OF SUBSTRATE BINDING</scope>
        <scope>KINETIC PARAMETERS</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Guanylyltransferase that catalyzes the synthesis of adenosylcobinamide-GDP (AdoCbi-GDP) from adenosylcobinamide-phosphate (AdoCbi-P) and GTP. Is involved in adenosylcobalamin biosynthesis. Binds one GTP per dimer. Cannot use other NTPs or GDP. Does not display AdoCbi kinase activity. Is also able to catalyze the condensation of 2-phospho-L-lactate (LP) with GTP in vitro to form PPi and (2S)-lactyl-2-diphospho-5'-guanosine (LPPG), but is much less efficient than CofC, the presumed enzyme catalyzing this reaction in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosylcob(III)inamide phosphate + GTP + H(+) = adenosylcob(III)inamide-GDP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22712"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58502"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.7.62"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1 2">18.4 uM for adenosylcobinamide phosphate</KM>
            <KM evidence="1 2">2.4 uM for GTP</KM>
            <KM evidence="1 2">6 mM for (2S)-2-phospholactate</KM>
            <Vmax evidence="1 2">115.0 nmol/min/mg enzyme with (2S)-2-phospholactate as substrate</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Binds GTP first, before binding AdoCbi-P.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.62"/>
    <dbReference type="EMBL" id="L77117">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D64439"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="PDB" id="2MZB">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-196"/>
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    <dbReference type="PDB" id="3RSB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B/C/D=1-196"/>
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    <dbReference type="STRING" id="243232.MJ_1117"/>
    <dbReference type="EnsemblBacteria" id="AAB99118">
        <property type="protein sequence ID" value="AAB99118"/>
        <property type="gene ID" value="MJ_1117"/>
    </dbReference>
    <dbReference type="GeneID" id="1452013"/>
    <dbReference type="KEGG" id="mja:MJ_1117"/>
    <dbReference type="eggNOG" id="arCOG01871">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098907_0_0_2"/>
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    <dbReference type="OMA" id="GMGEKPC"/>
    <dbReference type="OrthoDB" id="90478at2157"/>
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    <dbReference type="BRENDA" id="2.7.7.62">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58517"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="EvolutionaryTrace" id="Q58517"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008820">
        <property type="term" value="F:cobinamide phosphate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005245">
        <property type="entry name" value="CHP00454"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="SSF53448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00454">
        <property type="entry name" value="TIGR00454"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107174" description="Adenosylcobinamide-phosphate guanylyltransferase">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
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    </feature>
    <feature type="helix" evidence="3">
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    <feature type="helix" evidence="3">
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="39"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="45"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="64"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="69"/>
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        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="86"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="95"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="129"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="166"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="181"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="18260642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19489548"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3RSB"/>
        </source>
    </evidence>
    <sequence length="196" mass="21746" checksum="6E5F4F07A0F509D4" modified="1996-11-01" version="1">MDALIMAGGKGTRMGGVEKPLIKLCGRCLIDYVVSPLLKSKVNNIFIATSPNTPKTKEYINSAYKDYKNIVVIDTSGKGYIEDLNECIGYFSEPFLVVSSDLINLKSKIINSIVDYFYCIKAKTPDVEALAVMIPKEKYPNPSIDFNGLVPAGINVVSPKHGYQKEEIMVIDELIFNINTKDDLKLAEMLLKKDGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58533</accession>
    <name>Y1133_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1133</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1133</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UbiD family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64441">
        <property type="entry name" value="D64441"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58533"/>
    <dbReference type="SMR" id="Q58533"/>
    <dbReference type="STRING" id="243232.MJ_1133"/>
    <dbReference type="EnsemblBacteria" id="AAB99135">
        <property type="protein sequence ID" value="AAB99135"/>
        <property type="gene ID" value="MJ_1133"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1133"/>
    <dbReference type="eggNOG" id="arCOG01671">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023348_5_1_2"/>
    <dbReference type="InParanoid" id="Q58533"/>
    <dbReference type="OMA" id="TEGGCCW"/>
    <dbReference type="PhylomeDB" id="Q58533"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="PTHR30108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00148">
        <property type="entry name" value="TIGR00148"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000157383" description="Uncharacterized protein MJ1133">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="421" mass="47215" checksum="867974520F489996" modified="1996-11-01" version="1">MREIINKLNPIIIDKADKKFGVSRILKKYDGKPVYIKDVNGFEVVGNLCSRETLSKIFNVKKEDFIFFMLDAMEKEKEGKLKINNKLKEKYIVEIPENIKNWPIPIYYEKDAGAYITSGVVVVYDKDYGYNLSIHRILVKDDYLVIRMVEQRHLHFLYNKALKEKGYLDVAIVIGVHPAVLLAGSTSADITFDELKFAAALLGGEIGVFELDNGLLVPEAEFIIEGKILPEVDDEGPFVDITGTYDIVRKQPIIKIEKLYRKEKPIFHALLPGGIEHKTLMGMPQEPRILKGVRNTVPTVKNIVLTEGGCCWLHAVVQIEKRTEGDGKNAILAAFASHPSLKHVIVVDDDINIFDINDVEYAIATRVQGDKDIVIISGAKGSSLDPSSDLKNKLTAKVGVDATMSLIKGREHFERAKIPDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58542</accession>
    <name>ARSA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative arsenical pump-driving ATPase</fullName>
            <ecNumber>7.3.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Arsenical resistance ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Arsenite-translocating ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Arsenite-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1142</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Anion-transporting ATPase. Catalyzes the extrusion of arsenite.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>arsenite(in) + ATP + H2O = ADP + arsenite(out) + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29242"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.7"/>
        </reaction>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-7584233">
            <id>Q58542</id>
        </interactant>
        <interactant intactId="EBI-7584233">
            <id>Q58542</id>
            <label>MJ1142</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the arsA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.7"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64442">
        <property type="entry name" value="E64442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870653.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3UG6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.30 A"/>
        <property type="chains" value="A/B/C/D=1-349"/>
    </dbReference>
    <dbReference type="PDB" id="3UG7">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B/C/D=1-349"/>
    </dbReference>
    <dbReference type="PDBsum" id="3UG6"/>
    <dbReference type="PDBsum" id="3UG7"/>
    <dbReference type="AlphaFoldDB" id="Q58542"/>
    <dbReference type="SMR" id="Q58542"/>
    <dbReference type="IntAct" id="Q58542">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="MINT" id="Q58542"/>
    <dbReference type="STRING" id="243232.MJ_1142"/>
    <dbReference type="TCDB" id="3.A.19.1.3">
        <property type="family name" value="the guided entry of tail anchored protein (get) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99142">
        <property type="protein sequence ID" value="AAB99142"/>
        <property type="gene ID" value="MJ_1142"/>
    </dbReference>
    <dbReference type="GeneID" id="1452038"/>
    <dbReference type="KEGG" id="mja:MJ_1142"/>
    <dbReference type="eggNOG" id="arCOG02849">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040761_4_0_2"/>
    <dbReference type="InParanoid" id="Q58542"/>
    <dbReference type="OMA" id="MDAPYEF"/>
    <dbReference type="OrthoDB" id="84406at2157"/>
    <dbReference type="PhylomeDB" id="Q58542"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015446">
        <property type="term" value="F:ATPase-coupled arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="Anion-transp_ATPase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="PTHR10803"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0059">Arsenical resistance</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <feature type="chain" id="PRO_0000152257" description="Putative arsenical pump-driving ATPase">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="13"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="39"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="57"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="67"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="87"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="115"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="153"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="165"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="171"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="211"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="235"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="238"/>
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        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="248"/>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="268"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="307"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3UG6"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3UG7"/>
        </source>
    </evidence>
    <sequence length="349" mass="39919" checksum="00041F603DFDD5A3" modified="1996-11-01" version="1">MLSKIKDSINSLRGITEKKLEKKDGTKYIMFGGKGGVGKTTMSAATGVYLAEKGLKVVIVSTDPAHSLRDIFEQEFGHEPTKVKGYDNLYVVEIDPQKAMEEYKEKLKAQIEENPFLGEMLEDQLEMAALSPGTDESAAFDVFLKYMDSNEFDVVIFDTAPTGHTLRFLGMPEVMDKYMTKLIKLRKQMSGFMKMMKKLLPFGGKDEDIDYDKMLEELEKMKERIVRARNILSDPERTAFRLVVIPEEMSILESERAMKALQKYGIPIDAVIVNQLIPEDVQCDFCRARRELQLKRLEMIKEKFGDKVIAYVPLLRTEAKGIETLKQIAKILYGEEEKEEQKIEQKVGQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58549</accession>
    <name>ADPP_METJA</name>
    <protein>
        <recommendedName>
            <fullName>ADP-ribose pyrophosphatase</fullName>
            <ecNumber>3.6.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ADP-ribose diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ADP-ribose phosphohydrolase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Adenosine diphosphoribose pyrophosphatase</fullName>
            <shortName>ADPR-PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">nudF</name>
        <name type="ordered locus">MJ1149</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="J. Biol. Chem." volume="273" first="20924" last="20928">
            <title>Identification and characterization of the Nudix hydrolase from the Archaeon, Methanococcus jannaschii, as a highly specific ADP-ribose pyrophosphatase.</title>
            <authorList>
                <person name="Sheikh S."/>
                <person name="O'Handley S.F."/>
                <person name="Dunn C.A."/>
                <person name="Bessman M.J."/>
            </authorList>
            <dbReference type="PubMed" id="9694840"/>
            <dbReference type="DOI" id="10.1074/jbc.273.33.20924"/>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ADP-D-ribose + H2O = AMP + D-ribose 5-phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:10412"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57967"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text>Optimum pH is 4.85.</text>
        </phDependence>
        <temperatureDependence>
            <text>Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Nudix hydrolase family. NudF subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.13"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64443">
        <property type="entry name" value="D64443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870660.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58549"/>
    <dbReference type="SMR" id="Q58549"/>
    <dbReference type="STRING" id="243232.MJ_1149"/>
    <dbReference type="EnsemblBacteria" id="AAB99149">
        <property type="protein sequence ID" value="AAB99149"/>
        <property type="gene ID" value="MJ_1149"/>
    </dbReference>
    <dbReference type="GeneID" id="1452045"/>
    <dbReference type="KEGG" id="mja:MJ_1149"/>
    <dbReference type="eggNOG" id="arCOG01075">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037162_20_3_2"/>
    <dbReference type="InParanoid" id="Q58549"/>
    <dbReference type="OMA" id="NRDPRGH"/>
    <dbReference type="OrthoDB" id="116493at2157"/>
    <dbReference type="PhylomeDB" id="Q58549"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="SSF55811"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000057047" description="ADP-ribose pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="38"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Nudix box" evidence="3">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="12"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="169" mass="19144" checksum="1966F84445658B00" modified="1996-11-01" version="1">MAKKCFCISGKIFALNLFGKRYSKKIIKKRDLKKYRLYLHPAVAVDGIIEKDNKILLIKRKNNPFKGCFALPGGFVECGETVEEAVVREIKEETGLIPKVKSLLGVYSSPDRDPRGHVISIVFILDVIGGELKAGDDAKEAEFFDLNNLPKLAFDHEKIIKDYMRWKNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58578</accession>
    <name>Y1177_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative transport protein MJ1177</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1177</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64447">
        <property type="entry name" value="A64447"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870691.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58578"/>
    <dbReference type="STRING" id="243232.MJ_1177"/>
    <dbReference type="DNASU" id="1452076"/>
    <dbReference type="EnsemblBacteria" id="AAB99180">
        <property type="protein sequence ID" value="AAB99180"/>
        <property type="gene ID" value="MJ_1177"/>
    </dbReference>
    <dbReference type="GeneID" id="1452076"/>
    <dbReference type="KEGG" id="mja:MJ_1177"/>
    <dbReference type="eggNOG" id="arCOG02642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041771_3_0_2"/>
    <dbReference type="InParanoid" id="Q58578"/>
    <dbReference type="OMA" id="EIAYVYQ"/>
    <dbReference type="OrthoDB" id="79655at2157"/>
    <dbReference type="PhylomeDB" id="Q58578"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="PTHR21716"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000148326" description="Putative transport protein MJ1177">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="234"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="259"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="334" mass="38445" checksum="FD7429A229130200" modified="1996-11-01" version="1">MRFEEFKYVRKGVIVGLLIMLLYIIWPFIDVLAYSCAFAYMALPVYNILRKKFNKTISAGLAISIYILPIMTITIYALLTFMEIILSFNTKSIEPYINEILSIYNSFMLERIINNEQIIAKYIDEFIKYLVSQFSGKIIDVGYLIVKVIMVLFLTFYFLRDGDKAKNLIISFVPDEYKEKMRIYLSYLHDSYKNLFISCVSLSIIITILSYIGYLILGVPYAELFAIITGIFALLPILGGWMVYISIAIYFFLIHDYTKAVFMFIYGELFLSIAPDFVIRPYLVKKEVDIHPVLVVIAFLMAPLSLGLSGFAIGPLVVGALNAFYLAKYRDKKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58584</accession>
    <name>RISC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Riboflavin synthase</fullName>
            <ecNumber>2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ribC</name>
        <name type="ordered locus">MJ1184</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64447">
        <property type="entry name" value="G64447"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870697.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2B98">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D/E=1-156"/>
    </dbReference>
    <dbReference type="PDB" id="2B99">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.22 A"/>
        <property type="chains" value="A/B/C/D/E=1-156"/>
    </dbReference>
    <dbReference type="PDB" id="4Y7J">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.10 A"/>
        <property type="chains" value="A/B/C/D/E=1-156"/>
    </dbReference>
    <dbReference type="PDB" id="4Y7K">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.50 A"/>
        <property type="chains" value="A/B/C/D/E=2-156"/>
    </dbReference>
    <dbReference type="PDB" id="5IM5">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.70 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I/J/K/M/O/Q/S=1-156"/>
    </dbReference>
    <dbReference type="PDBsum" id="2B98"/>
    <dbReference type="PDBsum" id="2B99"/>
    <dbReference type="PDBsum" id="4Y7J"/>
    <dbReference type="PDBsum" id="4Y7K"/>
    <dbReference type="PDBsum" id="5IM5"/>
    <dbReference type="AlphaFoldDB" id="Q58584"/>
    <dbReference type="SMR" id="Q58584"/>
    <dbReference type="STRING" id="243232.MJ_1184"/>
    <dbReference type="EnsemblBacteria" id="AAB99185">
        <property type="protein sequence ID" value="AAB99185"/>
        <property type="gene ID" value="MJ_1184"/>
    </dbReference>
    <dbReference type="GeneID" id="1452082"/>
    <dbReference type="KEGG" id="mja:MJ_1184"/>
    <dbReference type="eggNOG" id="arCOG01322">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1682776_0_0_2"/>
    <dbReference type="InParanoid" id="Q58584"/>
    <dbReference type="OMA" id="QLMTNTH"/>
    <dbReference type="OrthoDB" id="104564at2157"/>
    <dbReference type="PhylomeDB" id="Q58584"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14602"/>
    <dbReference type="BRENDA" id="2.5.1.9">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00405"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58584"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09210">
        <property type="entry name" value="Riboflavin_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006399">
        <property type="entry name" value="Ribfl_synth_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015750">
        <property type="entry name" value="Ribfl_synth_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01506">
        <property type="entry name" value="ribC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134860" description="Riboflavin synthase">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="4"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="33"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="42"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="59"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="95"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="133"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="139"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="146"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2B98"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2B99"/>
        </source>
    </evidence>
    <sequence length="156" mass="17495" checksum="6D65112C6B1320F5" modified="1996-11-01" version="1">MTKKVGIVDTTFARVDMASIAIKKLKELSPNIKIIRKTVPGIKDLPVACKKLLEEEGCDIVMALGMPGKAEKDKVCAHEASLGLMLAQLMTNKHIIEVFVHEDEAKDDKELDWLAKRRAEEHAENVYYLLFKPEYLTRMAGKGLRQGFEDAGPARE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58592</accession>
    <name>VHUA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>F420-non-reducing hydrogenase vhu subunit A</fullName>
            <ecNumber>1.12.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vhuA</name>
        <name type="ordered locus">MJ1192</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">The F420-non-reducing hydrogenase vhu is composed of four subunits; VhuA, VhuD, VhuG and VhuU.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The large subunit of Vhu is split into VhuA and VhuU. Each contributes two ligands to the [NiFeSe] center.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <dbReference type="EC" id="1.12.99.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64448">
        <property type="entry name" value="G64448"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870705.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58592"/>
    <dbReference type="SMR" id="Q58592"/>
    <dbReference type="STRING" id="243232.MJ_1192"/>
    <dbReference type="EnsemblBacteria" id="AAB99194">
        <property type="protein sequence ID" value="AAB99194"/>
        <property type="gene ID" value="MJ_1192"/>
    </dbReference>
    <dbReference type="GeneID" id="1452611"/>
    <dbReference type="KEGG" id="mja:MJ_1192"/>
    <dbReference type="eggNOG" id="arCOG01549">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044556_0_0_2"/>
    <dbReference type="InParanoid" id="Q58592"/>
    <dbReference type="OMA" id="QNNLAMN"/>
    <dbReference type="OrthoDB" id="32760at2157"/>
    <dbReference type="PhylomeDB" id="Q58592"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000199730" description="F420-non-reducing hydrogenase vhu subunit A">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="418" mass="46652" checksum="0BF7333C53360FA4" modified="1996-11-01" version="1">MGKIVIEPLSRLEGHGKVTITLDENGKPKDVKLHITALRGFEQFVVGRPAEEVPRIVPRICGICQTAHHLASVKAIDAAWGVEIPEPAKKLRELMHIGNMIHSHALHFYFLAAPDFVLGPDADPAIRNIVGVIDKAPDVAKQAIALRKFGQKIVEAVGGKAIHPVTGIPGGQAKRLTEEERDELLKDADQMIEYAKNGVELIKQLNEQYMEQIKTLGVIDTYYLGLVKDGKHNFYDDTLRFLSPDGKEKVEFKPEEYLNYIGEYVVPYNYVKHPYYKKVGYPEGVYRVGPLAMLNVCDEMETPLAEEYRKEFLEIFGFPANQSLAYNHARLIELVEACEKAKILLEDNDITSDDIKADVEPKAGNGVGVVYAPRGVLIHNYETDENGIVVKANMIVATTHNVPTMEKAIQQAAQVIFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q58643</accession>
    <name>SUCD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="1">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="1">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">sucD</name>
        <name type="ordered locus">MJ1246</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2007-04" db="PDB data bank">
            <title>Crystal structure of succinyl-CoA synthetase alpha chain from Methanocaldococcus jannaschii DSM 2661.</title>
            <authorList>
                <person name="Niwa H."/>
                <person name="Kuramitsu S."/>
                <person name="Yokoyama S."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + CoA + succinate = ADP + phosphate + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64455">
        <property type="entry name" value="E64455"/>
    </dbReference>
    <dbReference type="PDB" id="2YV1">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=1-294"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YV1"/>
    <dbReference type="AlphaFoldDB" id="Q58643"/>
    <dbReference type="SMR" id="Q58643"/>
    <dbReference type="STRING" id="243232.MJ_1246"/>
    <dbReference type="EnsemblBacteria" id="AAB99250">
        <property type="protein sequence ID" value="AAB99250"/>
        <property type="gene ID" value="MJ_1246"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1246"/>
    <dbReference type="eggNOG" id="arCOG01339">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052104_0_0_2"/>
    <dbReference type="InParanoid" id="Q58643"/>
    <dbReference type="OMA" id="VIICITE"/>
    <dbReference type="PhylomeDB" id="Q58643"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q58643"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="CoA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="SSF52210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="chain" id="PRO_0000102810" description="Succinate--CoA ligase [ADP-forming] subunit alpha">
        <location>
            <begin position="1"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="1">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="25"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="20"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="40"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="56"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="62"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="73"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="131"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="138"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="145"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="150"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="161"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="177"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="185"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="193"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="219"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="234"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="283"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2YV1"/>
        </source>
    </evidence>
    <sequence length="294" mass="30923" checksum="97BAAE1C0D311E6D" modified="1997-11-01" version="1">MVLRDKMILLDENTKAIVQGITGRQGSFHTKKMLECGTKIVGGVTPGKGGQNVHGVPVFDTVKEAVKETDANASVIFVPAPFAKDAVFEAIDAGIELIVVITEHIPVHDTMEFVNYAEDVGVKIIGPNTPGIASPKVGKLGIIPMEVLKEGSVGMVSRSGTLTYEIAHQIKKAGFGVSTCVGIGGDPIVGLRYKEVLDLFEKDDETEAIVMIGEIGGGAEEEAAKFIEKMKKPVIGYIAGQSAPEGKRMGHAGAIVEKGKGTAESKMKALEEAGAYVAKNISDIPKLLAGILGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58662</accession>
    <name>Y1266_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1266</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1266</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64458">
        <property type="entry name" value="A64458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870779.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58662"/>
    <dbReference type="SMR" id="Q58662"/>
    <dbReference type="STRING" id="243232.MJ_1266"/>
    <dbReference type="EnsemblBacteria" id="AAB99272">
        <property type="protein sequence ID" value="AAB99272"/>
        <property type="gene ID" value="MJ_1266"/>
    </dbReference>
    <dbReference type="GeneID" id="1452164"/>
    <dbReference type="KEGG" id="mja:MJ_1266"/>
    <dbReference type="eggNOG" id="arCOG01021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027128_5_1_2"/>
    <dbReference type="InParanoid" id="Q58662"/>
    <dbReference type="OMA" id="MFQSETD"/>
    <dbReference type="OrthoDB" id="23746at2157"/>
    <dbReference type="PhylomeDB" id="Q58662"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028081">
        <property type="entry name" value="Leu-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13458">
        <property type="entry name" value="Peripla_BP_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="SSF53822"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014010" description="Uncharacterized protein MJ1266">
        <location>
            <begin position="22"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="417" mass="46249" checksum="DD8E13FDB556EF40" modified="1996-11-01" version="1" precursor="true">MPYYWGAILIGGVFLAGCTQNQETTTTQSGSKENVIKVGLLVDLSGGLATYGNNEKHICEIAEEKINKYFEEKGMPYKVKLYVEDTRADPNICLQKVQALHAQGITFFLGPMASGEVKNIKGFINSNKIVIISPSSTAPPQMLGFRTPEEKKYVFRFVPTDNFQGNAIGDVAKQLGLKNVIVIYRKDAWGDGLERATVEKLKANGINIIDEIPYDPNIGDWSPIIQTTTNKIAGKGNDTGVIFIGYEEVATLLSQIDDNSPLLKHVWIGCDGTANSKKVLEEAKNKAVKVKLYSTMFQSETDEAEKIKEEFKKRGYGEPDQYALNVYDAFWVGAISYAEMLNKTGGKYDADLLSKLIKENTVKYSEGQFGVKSVTGYIKLNEWNDRASGNYGIFAVTEDGWKLVGVWDSTTGKINWK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58695</accession>
    <name>BIOD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="1">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DTB synthetase</fullName>
            <shortName evidence="1">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bioD</name>
        <name type="ordered locus">MJ1299</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99306" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99306.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64462">
        <property type="entry name" value="B64462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496780.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58695"/>
    <dbReference type="SMR" id="Q58695"/>
    <dbReference type="STRING" id="243232.MJ_1299"/>
    <dbReference type="EnsemblBacteria" id="AAB99306">
        <property type="protein sequence ID" value="AAB99306"/>
        <property type="gene ID" value="MJ_1299"/>
    </dbReference>
    <dbReference type="GeneID" id="1452201"/>
    <dbReference type="KEGG" id="mja:MJ_1299"/>
    <dbReference type="eggNOG" id="arCOG00100">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072551_2_0_2"/>
    <dbReference type="InParanoid" id="Q58695"/>
    <dbReference type="OMA" id="SPHWAAE"/>
    <dbReference type="OrthoDB" id="82623at2157"/>
    <dbReference type="PhylomeDB" id="Q58695"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="PTHR43210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000188005" description="ATP-dependent dethiobiotin synthetase BioD">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="169"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="214" mass="23935" checksum="0E6AA4C9FA7D39C4" modified="2001-04-27" version="2">MIFITGTDTGIGKTYVSSILAENLKKMGINVGYLKPVETGGREDTLTLKNILNTDDDLDLMNPINLKLPLSPNIAFDVENSPLTLDEIKEKIKNAYETLKEKYDFLIVEGAGGVCVPIKEDFLMSDLIKFLGLDAVVVSRPNLGTINHTLLTVEHLRNKGINVRGVIINCITDLSEVLYYEKTFETIEKVGNIEIIGIVKSREDFEIDFEKILR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58709</accession>
    <name>Y1313_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1313</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1313</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To M.thermoautotrophicum MTH1421.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64463">
        <property type="entry name" value="H64463"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870830.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58709"/>
    <dbReference type="SMR" id="Q58709"/>
    <dbReference type="STRING" id="243232.MJ_1313"/>
    <dbReference type="PRIDE" id="Q58709"/>
    <dbReference type="EnsemblBacteria" id="AAB99320">
        <property type="protein sequence ID" value="AAB99320"/>
        <property type="gene ID" value="MJ_1313"/>
    </dbReference>
    <dbReference type="GeneID" id="1452215"/>
    <dbReference type="KEGG" id="mja:MJ_1313"/>
    <dbReference type="eggNOG" id="arCOG04278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018825_1_0_2"/>
    <dbReference type="InParanoid" id="Q58709"/>
    <dbReference type="OMA" id="CTCTPYL"/>
    <dbReference type="OrthoDB" id="31127at2157"/>
    <dbReference type="PhylomeDB" id="Q58709"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01355">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007506">
        <property type="entry name" value="AcnX_catalytic_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04412">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107270" description="Uncharacterized protein MJ1313">
        <location>
            <begin position="1"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="163"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="403" mass="44601" checksum="DDA9EB7CA167F348" modified="1996-11-01" version="1">MYLTKEEEKILDGEYGEVLRRCMNLLVSLGDIYGADKLIPISSAQISGVSYKTIKDIGLEFLEDFAKEDVKVKVYATLNPAGMDLDIWRELGIDEKFAKKQLRIIEAFKKMEVEISCTCTPYLTGNLPRFGEHISWAESSAVSFANSVLGAKTNREGGPSALAAAIIGKTPYYGYHLDENRKTTHIIELDGQLISNFKYGESFYGALGYLVGKIVKNGIPYFENLYKLNPNNDNLKSLGAAMAASGGIALYHAKNLTAECRVKEVVNDKIEKISIGVEEIKEAYEKLNTTNEEPDLICIGCPHCSLMEIKKIAELLKNKKLNADLWVCCSLHIKAIADRMGYTKIIEKAGGKVVKDTCMVVSPIEDLGYKRVATNSGKAAVYLPSFCKSEVIFGDIEELLKGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-20" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58712</accession>
    <name>Y1316_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0248 protein MJ1316</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1316</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0248 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64464">
        <property type="entry name" value="C64464"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58712"/>
    <dbReference type="STRING" id="243232.MJ_1316"/>
    <dbReference type="EnsemblBacteria" id="AAB99323">
        <property type="protein sequence ID" value="AAB99323"/>
        <property type="gene ID" value="MJ_1316"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1316"/>
    <dbReference type="eggNOG" id="arCOG01302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172276_3_1_2"/>
    <dbReference type="InParanoid" id="Q58712"/>
    <dbReference type="OMA" id="TQIPYHR"/>
    <dbReference type="PhylomeDB" id="Q58712"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01245">
        <property type="entry name" value="UPF0248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040459">
        <property type="entry name" value="MJ1316"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007547">
        <property type="entry name" value="UPF0248"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04457">
        <property type="entry name" value="MJ1316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000053413" description="UPF0248 protein MJ1316">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01245"/>
        </source>
    </evidence>
    <sequence length="80" mass="9683" checksum="D39072CF898CC1CE" modified="1996-11-01" version="1">MGIFMLKEILNKIFWHPDYKREDFEVVILHRGAEENKKAISLDDVELKGNYLIYFDTYIPLHRILEIRNKKTGEILYKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58719</accession>
    <name>MRE11_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mre11</name>
        <name type="ordered locus">MJ1323</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-10107692">
            <id>Q58719</id>
        </interactant>
        <interactant intactId="EBI-10107692">
            <id>Q58719</id>
            <label>mre11</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>3</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64465">
        <property type="entry name" value="B64465"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870840.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3AUZ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.21 A"/>
        <property type="chains" value="A=1-313"/>
    </dbReference>
    <dbReference type="PDB" id="3AV0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.10 A"/>
        <property type="chains" value="A=1-366"/>
    </dbReference>
    <dbReference type="PDB" id="4TUG">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.55 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="4TUI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.59 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-333"/>
    </dbReference>
    <dbReference type="PDB" id="5DNY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.11 A"/>
        <property type="chains" value="A/C=1-366"/>
    </dbReference>
    <dbReference type="PDB" id="5F3W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.11 A"/>
        <property type="chains" value="A/C=1-366"/>
    </dbReference>
    <dbReference type="PDBsum" id="3AUZ"/>
    <dbReference type="PDBsum" id="3AV0"/>
    <dbReference type="PDBsum" id="4TUG"/>
    <dbReference type="PDBsum" id="4TUI"/>
    <dbReference type="PDBsum" id="5DNY"/>
    <dbReference type="PDBsum" id="5F3W"/>
    <dbReference type="AlphaFoldDB" id="Q58719"/>
    <dbReference type="SMR" id="Q58719"/>
    <dbReference type="MINT" id="Q58719"/>
    <dbReference type="STRING" id="243232.MJ_1323"/>
    <dbReference type="EnsemblBacteria" id="AAB99332">
        <property type="protein sequence ID" value="AAB99332"/>
        <property type="gene ID" value="MJ_1323"/>
    </dbReference>
    <dbReference type="GeneID" id="1452225"/>
    <dbReference type="KEGG" id="mja:MJ_1323"/>
    <dbReference type="eggNOG" id="arCOG00397">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026621_5_2_2"/>
    <dbReference type="InParanoid" id="Q58719"/>
    <dbReference type="OMA" id="YRQYNLD"/>
    <dbReference type="OrthoDB" id="25870at2157"/>
    <dbReference type="PhylomeDB" id="Q58719"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004529">
        <property type="term" value="F:exodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000353"/>
        <property type="project" value="IntAct"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP_ApaH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000138689" description="DNA double-strand break repair protein Mre11">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="2"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="15"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="42"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="50"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="58"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="77"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="89"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="96"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="104"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="108"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="118"/>
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    <evidence type="ECO:0000255" key="1">
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    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3AV0"/>
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    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5DNY"/>
        </source>
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    <sequence length="366" mass="43132" checksum="EFF6ADAD43EB5AFC" modified="1996-11-01" version="1">MMFVHIADNHLGYRQYNLDDREKDIYDSFKLCIKKILEIKPDVVLHSGDLFNDLRPPVKALRIAMQAFKKLHENNIKVYIVAGNHEMPRRLGEESPLALLKDYVKILDGKDVINVNGEEIFICGTYYHKKSKREEMLDKLKNFESEAKNYKKKILMLHQGINPYIPLDYELEHFDLPKFSYYALGHIHKRILERFNDGILAYSGSTEIIYRNEYEDYKKEGKGFYLVDFSGNDLDISDIEKIDIECREFVEVNIKDKKSFNEAVNKIERCKNKPVVFGKIKREFKPWFDTLKDKILINKAIIVDDEFIDMPDNVDIESLNIKELLVDYANRQGIDGDLVLSLYKALLNNENWKELLDEYYNTKFRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58747</accession>
    <name>NADE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="1">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nadE</name>
        <name type="ordered locus">MJ1352</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + deamido-NAD(+) + NH4(+) = AMP + diphosphate + H(+) + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the NAD synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64468">
        <property type="entry name" value="G64468"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58747"/>
    <dbReference type="SMR" id="Q58747"/>
    <dbReference type="STRING" id="243232.MJ_1352"/>
    <dbReference type="EnsemblBacteria" id="AAB99363">
        <property type="protein sequence ID" value="AAB99363"/>
        <property type="gene ID" value="MJ_1352"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1352"/>
    <dbReference type="eggNOG" id="arCOG00069">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059327_1_1_2"/>
    <dbReference type="InParanoid" id="Q58747"/>
    <dbReference type="OMA" id="MAFLYDY"/>
    <dbReference type="PhylomeDB" id="Q58747"/>
    <dbReference type="BRENDA" id="6.3.1.5">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="PTHR23090"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152224" description="NH(3)-dependent NAD(+) synthetase">
        <location>
            <begin position="1"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="249"/>
            <end position="250"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="259" mass="28982" checksum="BAFD1B7ACAF951BF" modified="1996-11-01" version="1">MLWGESMEEIVNKITKFIREKVEEANANGVVVGLSGGIDSSVTAYLCVKALGKDKVLGLIMPEKNTNPKDVEHAKMVAENLGIKYIISDITDILKAFGAGGYVPTREFDKIADGNLKARIRMCILYYFANKYNLLVAGTSNKSEIYVGYGTKHGDIACDIRPIGNLFKTEVKKLAKYIGVPKEIIEKPPSAGLWEGQTDEEELDIKYETLDTILKLYEKGKTPEEIHKETNIPLETINYVFDLIKKNEHKRTLPPTPEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58752</accession>
    <name>MJK2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable potassium channel protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>MjK2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1357</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="FEBS Lett." volume="547" first="165" last="169">
            <title>MjK1, a K+ channel from M. jannaschii, mediates K+ uptake and K+ sensitivity in E. coli.</title>
            <authorList>
                <person name="Hellmer J."/>
                <person name="Zeilinger C."/>
            </authorList>
            <dbReference type="PubMed" id="12860407"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(03)00706-3"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Probable potassium channel protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64469">
        <property type="entry name" value="D64469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870874.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58752"/>
    <dbReference type="SMR" id="Q58752"/>
    <dbReference type="TCDB" id="1.A.1.13.10">
        <property type="family name" value="the voltage-gated ion channel (vic) superfamily"/>
    </dbReference>
    <dbReference type="PRIDE" id="Q58752"/>
    <dbReference type="EnsemblBacteria" id="AAB99365">
        <property type="protein sequence ID" value="AAB99365"/>
        <property type="gene ID" value="MJ_1357"/>
    </dbReference>
    <dbReference type="GeneID" id="1452259"/>
    <dbReference type="KEGG" id="mja:MJ_1357"/>
    <dbReference type="eggNOG" id="arCOG01958">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_050982_0_1_2"/>
    <dbReference type="InParanoid" id="Q58752"/>
    <dbReference type="OMA" id="HIIVCGC"/>
    <dbReference type="OrthoDB" id="80000at2157"/>
    <dbReference type="PhylomeDB" id="Q58752"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005267">
        <property type="term" value="F:potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0631">Potassium channel</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000054100" description="Probable potassium channel protein 2">
        <location>
            <begin position="1"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="29"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="83"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="3">
        <location>
            <begin position="109"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="4">
        <location>
            <begin position="253"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Selectivity filter" evidence="1">
        <location>
            <begin position="46"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="12860407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="343" mass="38884" checksum="61231B0C001B54C4" modified="1997-11-01" version="1">METSKKLVIVAVLSITLILTYAYLISIIEGVDYFTALYFSVITITTTGYGDFTPKTFLGRTLTVVYLCVGVGIVMYLFSLIAEFIVEGKFEEFVRLKKMKNKIKTLKDHYIICGYGRLGKVVGEKFIEENIPFIAIDINEDVLKEEYEKYPDKFLYIVGDAKKEEVLKKAKIDKAKGLIATLPSDADNVFLTLTARELNPNILITAKADEKEAIRKLKIAGANRVVSPYLIGGLRMAEVSVRPGILDFLSTFIKIAKDEYEEDIELRKFVIEKDSELAYKSLKDANIRGKTGATILGIRREKEFCINPYPEFILKPGDVIYAFGTEENLKYLENLVKKKKKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58758</accession>
    <name>Y1363_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1363</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1363</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64470">
        <property type="entry name" value="B64470"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58758"/>
    <dbReference type="SMR" id="Q58758"/>
    <dbReference type="STRING" id="243232.MJ_1363"/>
    <dbReference type="EnsemblBacteria" id="AAB99371">
        <property type="protein sequence ID" value="AAB99371"/>
        <property type="gene ID" value="MJ_1363"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1363"/>
    <dbReference type="eggNOG" id="arCOG01553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055737_7_3_2"/>
    <dbReference type="InParanoid" id="Q58758"/>
    <dbReference type="OMA" id="WNPREAD"/>
    <dbReference type="PhylomeDB" id="Q58758"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000118784" description="Uncharacterized protein MJ1363">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="151" mass="16168" checksum="8A2910BB696F5F21" modified="1996-11-01" version="1">MTVMIKKIARKKCIHVMLVYTGGCNACDIEVVNAIFSPFYDAEQYNVFLTFNPREADILVVTGCVTKVVAESLRKIYEKIPEPKAVVAVGACALMGGVYKNIGGDLGTSDFVAGPVENIIPVDVKVPGCAPRPEDIIAGIVKALPKVIEGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q58781</accession>
    <name>Y1386_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1386</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1386</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64473">
        <property type="entry name" value="A64473"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58781"/>
    <dbReference type="STRING" id="243232.MJ_1386"/>
    <dbReference type="EnsemblBacteria" id="AAB99399">
        <property type="protein sequence ID" value="AAB99399"/>
        <property type="gene ID" value="MJ_1386"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1386"/>
    <dbReference type="HOGENOM" id="CLU_2678944_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107305" description="Uncharacterized protein MJ1386">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="74" mass="8517" checksum="03F777FB41325531" modified="1996-11-01" version="1">MKIYDAVVKTTFQISTSIFFDYIYFFDYKGMKMAEIFAVNNYTELKKIRRMITFGFTVLGLGIGMIFGDAGLDV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58793</accession>
    <name>Y1398_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ1398</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1398</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64474">
        <property type="entry name" value="E64474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870915.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58793"/>
    <dbReference type="SMR" id="Q58793"/>
    <dbReference type="STRING" id="243232.MJ_1398"/>
    <dbReference type="EnsemblBacteria" id="AAB99407">
        <property type="protein sequence ID" value="AAB99407"/>
        <property type="gene ID" value="MJ_1398"/>
    </dbReference>
    <dbReference type="GeneID" id="1452301"/>
    <dbReference type="KEGG" id="mja:MJ_1398"/>
    <dbReference type="eggNOG" id="arCOG02611">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_718896_0_0_2"/>
    <dbReference type="OMA" id="SHKDINT"/>
    <dbReference type="OrthoDB" id="48466at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000160635" description="Uncharacterized HTH-type transcriptional regulator MJ1398">
        <location>
            <begin position="1"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="1">
        <location>
            <begin position="235"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00340"/>
        </source>
    </evidence>
    <sequence length="391" mass="45530" checksum="68FE5DF4B1418989" modified="1996-11-01" version="1">MKKDTLLLIILILFCIVHVYGIYVHIYGINVSLENVSQYLYNTTVYLSYENGTPCFYIGEKKEFVIIPPYIKVDRDIAPLIKKLRFKIENKSYNLIITKKNVNTNKMAIIFTSINGSREVNGNKTIIWIKDPLKLKQSEVEALYELPEKGEVIARYKDGKPAAIKINNKIYVGFKPDEDVLANLIYIHIVKKTSNPLPYILFTVFLTLASLMLTFQETLKKKFLELISALASVKVFILSRINLLDEEKVLLNDTRREIYNYILDNPGCHLRELSKNLNKPVSTLTWHLRILEKANLIKSKKFGNRLIYYPADMDMRDLPLLYLKNETQKSIFEYLLKSPAHLRKIAKDLNLNVETVRYNLKKLENLGIVKSKEEGNRIVYYINESILKFHK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58802</accession>
    <name>Y1407_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0107 protein MJ1407</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1407</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0107 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64475">
        <property type="entry name" value="F64475"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870924.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58802"/>
    <dbReference type="SMR" id="Q58802"/>
    <dbReference type="STRING" id="243232.MJ_1407"/>
    <dbReference type="EnsemblBacteria" id="AAB99415">
        <property type="protein sequence ID" value="AAB99415"/>
        <property type="gene ID" value="MJ_1407"/>
    </dbReference>
    <dbReference type="GeneID" id="1452310"/>
    <dbReference type="KEGG" id="mja:MJ_1407"/>
    <dbReference type="eggNOG" id="arCOG04279">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141583_2_0_2"/>
    <dbReference type="InParanoid" id="Q58802"/>
    <dbReference type="OMA" id="CEPIVAT"/>
    <dbReference type="OrthoDB" id="108624at2157"/>
    <dbReference type="PhylomeDB" id="Q58802"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01356">
        <property type="entry name" value="AcnX_swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00078">
        <property type="entry name" value="UPF0107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020794">
        <property type="entry name" value="AcnX_sub2_archaea_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012016">
        <property type="entry name" value="AcnX_sub2_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002840">
        <property type="entry name" value="AcnX_swivel_put"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01989">
        <property type="entry name" value="DUF126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004966">
        <property type="entry name" value="UCP004966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152565" description="UPF0107 protein MJ1407">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="129" mass="13724" checksum="995D5A3F705C44D9" modified="1996-11-01" version="1">MELKGRSISKGIIEGIAIVSKKPFSFLGGVDEEGNIIDKDSDLYGQSLKGKIFVFPYGRGSTVGSYVIYGLAKRGILKGIVNKECEPIVATGAILGGIPLVDKIDIEEIKTGDRIVVDGNTGVVKILNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58816</accession>
    <name>CBIA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="1">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ni-sirohydrochlorin a,c-diamide synthase</fullName>
            <ecNumber evidence="1">6.3.5.12</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Ni-sirohydrochlorin a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiA</name>
        <name evidence="1" type="synonym">cfbB</name>
        <name type="ordered locus">MJ1421</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of Ni-sirohydrochlorin, using L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + cob(II)yrinate + 2 H2O + 2 L-glutamine = 2 ADP + cob(II)yrinate a,c diamide + 2 H(+) + 2 L-glutamate + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 ATP + 2 H2O + 2 L-glutamine + Ni-sirohydrochlorin = 2 ADP + 2 H(+) + 2 L-glutamate + Ni-sirohydrochlorin a,c-diamide + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:52896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="ChEBI" id="CHEBI:136887"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP, and cobyrinate or Ni-sirohydrochlorin, and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">The a and c carboxylates of cobyrinate and Ni-sirohydrochlorin are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CobB/CbiA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="1"/>
    <dbReference type="EC" id="6.3.5.12" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64477">
        <property type="entry name" value="D64477"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58816"/>
    <dbReference type="SMR" id="Q58816"/>
    <dbReference type="STRING" id="243232.MJ_1421"/>
    <dbReference type="EnsemblBacteria" id="AAB99432">
        <property type="protein sequence ID" value="AAB99432"/>
        <property type="gene ID" value="MJ_1421"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1421"/>
    <dbReference type="eggNOG" id="arCOG00106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022752_2_0_2"/>
    <dbReference type="InParanoid" id="Q58816"/>
    <dbReference type="OMA" id="QPFKCGP"/>
    <dbReference type="PhylomeDB" id="Q58816"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="PTHR43873"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141276" description="Cobyrinate a,c-diamide synthase">
        <location>
            <begin position="1"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="GATase cobBQ-type" evidence="1">
        <location>
            <begin position="248"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="1">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <sequence length="443" mass="50206" checksum="EA8D66D0FC9ED212" modified="1996-11-01" version="1">MIMKRVVIAGTSSEVGKTVISTGIMKALSKKYNVQGYKVGPDYIDPTYHTIATGNKSRNLDSFFMNKEQIKYLFQKHSKDKDISVIEGVRGLYEGISAIDDIGSTASVAKALDSPIILLVNAKSLTRSAIAIIKGFMSFDNVKIKGVIFNFVRSENHIKKLKDAMSYYLPDIEIIGFIPRNEDFKVEGRHLGLVPTPENLKEIESKIVLWGELVEKYLDLDKIVEIADEDFEEVDDVFLWEVNENYKKIAVAYDKAFNFYYWDNFEALKENKAKIEFFSPLKDSEVPDADILYIGGGYPELFKEELSRNKEMIESIKEFDGYIYGECGGLMYITKSIDNVPMVGLLNCSAVMTKHVQGLSYVKAEFLEDCLIGRKGLKFKGHEFHYSKLVNIKEERFAYKIERGRGIINNLDGIFNGKVLAGYLHNHAVANPYFASSMVNFGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58817</accession>
    <name>RFCS_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Replication factor C small subunit</fullName>
            <shortName>RFC small subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>Clamp loader small subunit</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Mja RFC-1 intein</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Mja RFC-2 intein</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Mja RFC-3 intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">rfcS</name>
        <name type="ordered locus">MJ1422</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="PTM">
        <text evidence="4">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The intein interrupts the potential ATP-binding site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64477">
        <property type="entry name" value="E64477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870940.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58817"/>
    <dbReference type="SMR" id="Q58817"/>
    <dbReference type="STRING" id="243232.MJ_1422"/>
    <dbReference type="MEROPS" id="N10.007"/>
    <dbReference type="PRIDE" id="Q58817"/>
    <dbReference type="EnsemblBacteria" id="AAB99433">
        <property type="protein sequence ID" value="AAB99433"/>
        <property type="gene ID" value="MJ_1422"/>
    </dbReference>
    <dbReference type="GeneID" id="1452326"/>
    <dbReference type="KEGG" id="mja:MJ_1422"/>
    <dbReference type="eggNOG" id="arCOG00469">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03158">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002046_0_0_2"/>
    <dbReference type="InParanoid" id="Q58817"/>
    <dbReference type="OMA" id="VSHNCNY"/>
    <dbReference type="OrthoDB" id="37207at2157"/>
    <dbReference type="PhylomeDB" id="Q58817"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="SSF48019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000030361" description="Replication factor C small subunit, 1st part" evidence="2">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030362" description="Mja RFC-1 intein" evidence="2">
        <location>
            <begin position="54"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030363" description="Replication factor C small subunit, 2nd part" evidence="2">
        <location>
            <begin position="602"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030364" description="Mja RFC-2 intein" evidence="2">
        <location>
            <begin position="627"/>
            <end position="1062"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030365" description="Replication factor C small subunit, 3rd part" evidence="2">
        <location>
            <begin position="1063"/>
            <end position="1124"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030366" description="Mja RFC-3 intein" evidence="2">
        <location>
            <begin position="1125"/>
            <end position="1667"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000030367" description="Replication factor C small subunit, 4th part" evidence="2">
        <location>
            <begin position="1668"/>
            <end position="1847"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease 1" evidence="3">
        <location>
            <begin position="179"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease 2" evidence="3">
        <location>
            <begin position="780"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease 3" evidence="3">
        <location>
            <begin position="1348"/>
            <end position="1508"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="1847" mass="214097" checksum="44EADB5B4D6DE64A" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58829</accession>
    <name>Y1434_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative DNA repair glycosylase MJ1434</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1434</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="2045" last="2055">
            <title>A novel uracil-DNA glycosylase family related to the helix-hairpin-helix DNA glycosylase superfamily.</title>
            <authorList>
                <person name="Chung J.H."/>
                <person name="Im E.K."/>
                <person name="Park H.Y."/>
                <person name="Kwon J.H."/>
                <person name="Lee S."/>
                <person name="Oh J."/>
                <person name="Hwang K.C."/>
                <person name="Lee J.H."/>
                <person name="Jang Y."/>
            </authorList>
            <dbReference type="PubMed" id="12682355"/>
            <dbReference type="DOI" id="10.1093/nar/gkg319"/>
        </citation>
        <scope>PRELIMINARY FUNCTION AS AN URACIL-DNA GLYCOSYLASE</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2010" name="Nucleic Acids Res." volume="38" first="5119" last="5129">
            <title>Helix-hairpin-helix protein MJ1434 from Methanocaldococcus jannaschii and EndoIV homologue TTC0482 from Thermus thermophilus HB27 do not process DNA uracil residues.</title>
            <authorList>
                <person name="Schomacher L."/>
                <person name="Smolorz S."/>
                <person name="Ciirdaeva E."/>
                <person name="Ber S."/>
                <person name="Kramer W."/>
                <person name="Fritz H.J."/>
            </authorList>
            <dbReference type="PubMed" id="20410075"/>
            <dbReference type="DOI" id="10.1093/nar/gkq270"/>
        </citation>
        <scope>SHOWS THAT IT HAS NO URACIL-DNA GLYCOSYLASE ACTIVITY</scope>
        <scope>MUTAGENESIS OF GLU-132</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2 3">Was originally thought to have uracil-DNA glycosylase activity, but further protein analysis show that it does not exhibit DNA uracil glycosylase activity when produced in an Ung-deficient Escherichia coli host.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks the lysine residue within the HhH motif critical for AP lyase activity.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64479">
        <property type="entry name" value="A64479"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58829"/>
    <dbReference type="SMR" id="Q58829"/>
    <dbReference type="STRING" id="243232.MJ_1434"/>
    <dbReference type="EnsemblBacteria" id="AAB99444">
        <property type="protein sequence ID" value="AAB99444"/>
        <property type="gene ID" value="MJ_1434"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1434"/>
    <dbReference type="eggNOG" id="arCOG00461">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012862_6_0_2"/>
    <dbReference type="InParanoid" id="Q58829"/>
    <dbReference type="OMA" id="GPQGWWP"/>
    <dbReference type="PhylomeDB" id="Q58829"/>
    <dbReference type="BRENDA" id="3.2.2.27">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="SSF48150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102244" description="Putative DNA repair glycosylase MJ1434">
        <location>
            <begin position="1"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Shows AP lyase activity." evidence="3">
        <original>E</original>
        <variation>K</variation>
        <location>
            <position position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0AB83"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12682355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="20410075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="220" mass="25938" checksum="BB3188BE152995F6" modified="1996-11-01" version="1">MKENKFEMIYKIYKILLDYYGHQNWWPAETRYEVVVGAILTQNTSWKNVERAINNLKMEDLLEEVKILNVDEDKLKELIRPAGFYNLKAKRLKNVTKFIVENYGNTEEMAKTDKDTLILRAELLSINGVGKETADSILLYALDRESFVVDAYTKRMFSRLGVINEKAKYDEIKEIFEKNLPKDLEIYKEYHALIVEHCKKFCRKKALCDNCPIKEFCLSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q58950</accession>
    <name>SYDND_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="1">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="1">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aspS</name>
        <name type="ordered locus">MJ1555</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx)</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64494">
        <property type="entry name" value="B64494"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58950"/>
    <dbReference type="SMR" id="Q58950"/>
    <dbReference type="STRING" id="243232.MJ_1555"/>
    <dbReference type="EnsemblBacteria" id="AAB99575">
        <property type="protein sequence ID" value="AAB99575"/>
        <property type="gene ID" value="MJ_1555"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1555"/>
    <dbReference type="eggNOG" id="arCOG00406">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004553_2_1_2"/>
    <dbReference type="InParanoid" id="Q58950"/>
    <dbReference type="OMA" id="WVHEIRD"/>
    <dbReference type="PhylomeDB" id="Q58950"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="PTHR43450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000110995" description="Aspartate--tRNA(Asp/Asn) ligase">
        <location>
            <begin position="1"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="1">
        <location>
            <begin position="198"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="228"/>
            <end position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="364"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="409"/>
            <end position="412"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="1">
        <location>
            <position position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <sequence length="438" mass="50350" checksum="13752FF19CBE5337" modified="1997-11-01" version="1">MVNKMKWRRTHYSADIKPEMDGQEVIIMGWVHSIRALGKIIFVILRDREGTVQIVAPKQKVGDELFSQIKKLGAEDVIAVKGKVIANEKAPNGFEILPLELEVINTAKRPLPLDPAEKVPAELDTRLENRFLDLRRPKVQAIFKIRSEMLKSVRNTLYNEGFIEVNTPKLVASCTEGGTELFPISYFEREAFLGQSPQLYKQMLMATGLDRVFEIAPIFRAEEHNTRRHLNEATSIDIEMAFADDKDAMDILEKVVYNAFVDVYENRKKEIETLGIEFELPPEKFDRITYDEAIDIANAKGVEISWGEDLSREAEKAIGEEMEGLYFITDWPSEIRPFYTMPDEKNPNICKAFDLMYKDLEISSGAQRIHLYDLLVENIKKKGLNPDGFTYYLEAFKYGMPPHAGWGLGADRFTMVLTQQENIRECVLFPRDRQRLTP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58964</accession>
    <name>Y1569_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative metal transport protein MJ1569</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1569</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>May be involved in metal transport.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CbiM family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64495">
        <property type="entry name" value="H64495"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871093.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58964"/>
    <dbReference type="SMR" id="Q58964"/>
    <dbReference type="STRING" id="243232.MJ_1569"/>
    <dbReference type="EnsemblBacteria" id="AAB99587">
        <property type="protein sequence ID" value="AAB99587"/>
        <property type="gene ID" value="MJ_1569"/>
    </dbReference>
    <dbReference type="GeneID" id="1452477"/>
    <dbReference type="KEGG" id="mja:MJ_1569"/>
    <dbReference type="eggNOG" id="arCOG02248">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052508_2_1_2"/>
    <dbReference type="InParanoid" id="Q58964"/>
    <dbReference type="OMA" id="GTCSHPC"/>
    <dbReference type="OrthoDB" id="92958at2157"/>
    <dbReference type="PhylomeDB" id="Q58964"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000041">
        <property type="term" value="P:transition metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000107416" description="Putative metal transport protein MJ1569">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="222" mass="23948" checksum="69AEE7C6D44C112B" modified="1996-11-01" version="1">MHIPDGYLGPITCAFFYLIMIPIWYKSIKELKKLDPRKLPLLGVLTAFSFLVMMFNLPVPDGTTAHMVGGTLIAILMDNPWVATIAISIVLIIQAIFFGDGGITCIGANCFNMGVVLPFVGYYVYKFLRDKVGEVIASGIGAYVGIVAAAIVAGFEFGLQPFIEPGYCPYPFTVSVPAMAFAHLITAGPAAAVVTAIVVWYVKKVRPDLFTSKEQQVSGVNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58985</accession>
    <name>Y1590_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1590</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1590</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64498">
        <property type="entry name" value="E64498"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58985"/>
    <dbReference type="STRING" id="243232.MJ_1590"/>
    <dbReference type="EnsemblBacteria" id="AAB99618">
        <property type="protein sequence ID" value="AAB99618"/>
        <property type="gene ID" value="MJ_1590"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1590"/>
    <dbReference type="eggNOG" id="arCOG05117">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2230392_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107431" description="Uncharacterized protein MJ1590">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="105" mass="11685" checksum="37A60722D7DCC2B3" modified="1996-11-01" version="1">MRISPLIAGLIGGFTAAILQALFKVFPPPAYGICIACHTRDLVNWIINHLFGTTLGMALVSKAFPVLTVVGIFIGALITTFIYKETELKQTHSPVYWFHIRNFSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q59039</accession>
    <name>QUEE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="1">CDG synthase</shortName>
            <ecNumber evidence="1">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">queE</name>
        <name type="ordered locus">MJ1645</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-deazaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="1">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64505">
        <property type="entry name" value="C64505"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871169.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59039"/>
    <dbReference type="SMR" id="Q59039"/>
    <dbReference type="STRING" id="243232.MJ_1645"/>
    <dbReference type="EnsemblBacteria" id="AAB99666">
        <property type="protein sequence ID" value="AAB99666"/>
        <property type="gene ID" value="MJ_1645"/>
    </dbReference>
    <dbReference type="GeneID" id="1452554"/>
    <dbReference type="KEGG" id="mja:MJ_1645"/>
    <dbReference type="eggNOG" id="arCOG02173">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066739_1_0_2"/>
    <dbReference type="InParanoid" id="Q59039"/>
    <dbReference type="OMA" id="IPQTHKM"/>
    <dbReference type="OrthoDB" id="64973at2157"/>
    <dbReference type="PhylomeDB" id="Q59039"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000370">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000107457" description="7-carboxy-7-deazaguanine synthase">
        <location>
            <begin position="1"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="15"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="243" mass="28280" checksum="E035C9BE4188BCA2" modified="1996-11-01" version="1">MIREIFNSIMGEGKYIGRRFIFVRFAGCPLNCVYCDEESKGYFNRVEKIPGSGEFETLQKMEIEDIINAIDKLKTPDLFAVSFTGGEPLLYHKQIKEIAEILKDKGYRTFLESNGMFPERIFYFDIASIDIKLKEHFEYIKDEDYEKLYKNELKTIKKLYNLNSDIYAKVVIMEETNIEDVKIIAKDLSDIGNITLCIQPVTPHGNIKSPSQRKLFEIMEACGEYLKDNVMLTIQMHKYLGML</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q59047</accession>
    <name>Y1653_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ribosomal RNA large subunit methyltransferase MJ1653</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1653</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. RlmI family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64506">
        <property type="entry name" value="C64506"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871177.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59047"/>
    <dbReference type="SMR" id="Q59047"/>
    <dbReference type="STRING" id="243232.MJ_1653"/>
    <dbReference type="EnsemblBacteria" id="AAB99674">
        <property type="protein sequence ID" value="AAB99674"/>
        <property type="gene ID" value="MJ_1653"/>
    </dbReference>
    <dbReference type="GeneID" id="1452562"/>
    <dbReference type="KEGG" id="mja:MJ_1653"/>
    <dbReference type="eggNOG" id="arCOG00032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014042_0_0_2"/>
    <dbReference type="InParanoid" id="Q59047"/>
    <dbReference type="OMA" id="DCFCHTG"/>
    <dbReference type="OrthoDB" id="59984at2157"/>
    <dbReference type="PhylomeDB" id="Q59047"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041532">
        <property type="entry name" value="RlmI_PUA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019614">
        <property type="entry name" value="SAM-dep_methyl-trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10672">
        <property type="entry name" value="Methyltrans_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17785">
        <property type="entry name" value="PUA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000213171" description="Putative ribosomal RNA large subunit methyltransferase MJ1653">
        <location>
            <begin position="1"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="385" mass="43763" checksum="27EC604EC4A435ED" modified="1996-11-01" version="1">MTTKLYVDFGGYSAIEKGNLIIPRDNILNKEDFDSIEIGEVVDIYSKRGKFLGRGFKNPKEVRIMTLRKEDLDENYIREKIIKANEYRLKLGFKDTYRMVYTQSDWLNGLVIDKYNDIATVQIFNYGIEKMKDVVVETLLDLGIDSIYEKSSGRNRKRAGLPEVEGILAGEKTETIIQEGEAKFKVTFDGQKTGFFLDQRENRLELEKFIKEGDRVLDICCYTGGFSVHAAIRGAEVVGVDLSKKALKLAEENMELNNIPKDRYEFIEGNAFKVMEEFIEDGEKFDVVILDPPAFAQSKKALKSAIKGYHMLNRFGAKLADRLLVTCSCSQPLEPDAFKALVIDACLKAKKWAKIIKYGSQSPDHPITSKGTEYLKCLFLSVEEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q59063</accession>
    <name>CSM3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system Cms endoribonuclease Csm3</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR type III A-associated RAMP protein Csm3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">csm3</name>
        <name type="ordered locus">MJ1669</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2015" name="J. Mol. Biol." volume="427" first="259" last="273">
            <title>Crystal structure of the Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex.</title>
            <authorList>
                <person name="Numata T."/>
                <person name="Inanaga H."/>
                <person name="Sato C."/>
                <person name="Osawa T."/>
            </authorList>
            <dbReference type="PubMed" id="25451598"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2014.09.029"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) IN COMPLEX WITH ZINC</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 43-ASP--PHE-45; ARG-109; 214-TYR-TYR-215 AND GLU-216</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities.</text>
    </comment>
    <comment type="function">
        <text evidence="1 2">This subunit has the target ssRNA endonuclease activity; it cleaves multiple sites in the target RNA at 6 nucleotide intervals (By similarity). The Csm3-Csm4 complex binds both crRNA and a non-specific RNA; Csm3 alone was not seen to bind RNA (PubMed:25451598).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Part of the Csm effector complex that includes Cas10, Csm2, Csm3, Csm4 and Csm5 (By similarity). Stable Csm3-Csm4 (which crystallizes as 2 heterodimers) and Cas10-Csm1-Csm3-Csm4 subcomplexes can be isolated; Cas10 and Csm3 probably do not directly interact (PubMed:25451598).</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">Encoded in a type III-A CRISPR locus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated Csm3 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64508">
        <property type="entry name" value="C64508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871193.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4QTS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.10 A"/>
        <property type="chains" value="C/D=1-248"/>
    </dbReference>
    <dbReference type="PDBsum" id="4QTS"/>
    <dbReference type="AlphaFoldDB" id="Q59063"/>
    <dbReference type="SMR" id="Q59063"/>
    <dbReference type="STRING" id="243232.MJ_1669"/>
    <dbReference type="EnsemblBacteria" id="AAB99689">
        <property type="protein sequence ID" value="AAB99689"/>
        <property type="gene ID" value="MJ_1669"/>
    </dbReference>
    <dbReference type="GeneID" id="1452578"/>
    <dbReference type="KEGG" id="mja:MJ_1669"/>
    <dbReference type="eggNOG" id="arCOG02658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067743_0_0_2"/>
    <dbReference type="InParanoid" id="Q59063"/>
    <dbReference type="OMA" id="ICKIFGP"/>
    <dbReference type="OrthoDB" id="98478at2157"/>
    <dbReference type="PhylomeDB" id="Q59063"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
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        <property type="entry name" value="CRISPR-assoc_RAMP_Csm3"/>
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        <property type="entry name" value="RAMPs"/>
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        <property type="entry name" value="cas7_TM1809"/>
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    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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    <keyword id="KW-0862">Zinc</keyword>
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            <end position="248"/>
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    <feature type="binding site" evidence="2 4">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 4">
        <location>
            <position position="83"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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    </feature>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
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    <feature type="mutagenesis site" description="Wild-type interaction with Csm4." evidence="2">
        <original>DAF</original>
        <variation>AAA</variation>
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            <begin position="43"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Wild-type interaction with Csm4." evidence="2">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No longer interacts with Csm4." evidence="2">
        <original>YY</original>
        <variation>AA</variation>
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            <begin position="214"/>
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    <feature type="mutagenesis site" description="Wild-type interaction with Csm4." evidence="2">
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    <feature type="strand" evidence="5">
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    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="234"/>
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    </feature>
    <evidence type="ECO:0000250" key="1">
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    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="25451598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PDB" id="4QTS"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="4QTS"/>
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    </evidence>
    <sequence length="248" mass="28159" checksum="C68C09C5FC256BB1" modified="1996-11-01" version="1">MENLTLKGKVILEGIIELETGMHIGGTKETLKIGGTDNPVIRDAFGRILIPGSSLKGKIRALLERKDGKYKEDGRGNYLPHDCGECEICKIFGPHDSKNIKEPVRVIVRDAYLQPEENKKDYDYLEIKVENTIDRLKGTTIKGGIRNMERVVAGSKFKFEVVFNIYKESDKELIKKFIEGMKLLEDDYLGGSGSRGYGKIKFRDIKLICKPKEYYEGNENSKKESDEVESLNELESELDKIWGGINFN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q60258</accession>
    <name>Y3534_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJECL34</fullName>
        </recommendedName>
    </protein>
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        <name type="ordered locus">MJECL34</name>
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanococcales</taxon>
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            <taxon>Methanocaldococcus</taxon>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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                <person name="Woese C.R."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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        </location>
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</entry>
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    <accession>Q60286</accession>
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        <recommendedName>
            <fullName>Uncharacterized protein MJECL27</fullName>
        </recommendedName>
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        <name type="ordered locus">MJECL27</name>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <text evidence="2">To M.jannaschii MJECS06.</text>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <sequence length="162" mass="18670" checksum="C2FBD6BAC47555D7" modified="1997-11-01" version="1">MDILGEIVNVDEYVEKLELQKNDIGFYKCPFCDYTNADAKVVRKHVKSKHLEEIEKELKKLESQKSKNNGKKQTGQKKQGKGKKQPKRVRETCVSTQERKDYVLFFCHNHKVRLHLANGEVLEGKCCCKDPYTVLVDVGKGDVVIVNKAYIVKYVPLDLEKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q60299</accession>
    <name>Y3544_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL44</name>
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    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
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        <lineage>
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            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64515">
        <property type="entry name" value="C64515"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890092.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60299"/>
    <dbReference type="STRING" id="243232.MJ_ECL44"/>
    <dbReference type="PRIDE" id="Q60299"/>
    <dbReference type="EnsemblBacteria" id="AAC37113">
        <property type="protein sequence ID" value="AAC37113"/>
        <property type="gene ID" value="MJ_ECL44"/>
    </dbReference>
    <dbReference type="GeneID" id="1450826"/>
    <dbReference type="KEGG" id="mja:MJ_ECL44"/>
    <dbReference type="eggNOG" id="arCOG03240">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_838406_0_0_2"/>
    <dbReference type="OrthoDB" id="2900at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001238">
        <property type="entry name" value="DNA-binding_RecF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32182:SF0">
        <property type="entry name" value="PTHR32182:SF0"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107523" description="Uncharacterized protein MJECL44">
        <location>
            <begin position="1"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="43"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="331" mass="38593" checksum="E88952E0D5FEFC3F" modified="1997-11-01" version="1">MFIKWGDRLMTKTLHLTKVTIRNFRSIKVAHINNIGDIAVFVGANESGKSNILKALNWFGTDKPLGGDDIPVEFLGKENKEYSKKPIIEAYFEIVNKDKFKEKIISNIKEILEVIKVDINEDILINFEDMLNKIQFLKFEKYADGLFKTYIYDDQLEDITKEFYDCFKNVISKKTPLDLFNLIYEEVLKEEVKKQNIPENQISNAVANIRGNPNFNNHYRKILDEIRTLNTFEEFYEICKRIENIVKSIPNTSVSISIPGRNIQLNPYNIFTEILTKFNSIVTIAFINLKPKFVYLDEEMELKGAVIKNTSWSNTLKEENKTTLLMPDFLE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="62" xmlns="http://uniprot.org/uniprot">
    <accession>Q60300</accession>
    <name>Y3401_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS01</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS01</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64516">
        <property type="entry name" value="A64516"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890094.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60300"/>
    <dbReference type="STRING" id="243232.MJ_ECS01"/>
    <dbReference type="EnsemblBacteria" id="AAC37059">
        <property type="protein sequence ID" value="AAC37059"/>
        <property type="gene ID" value="MJ_ECS01"/>
    </dbReference>
    <dbReference type="GeneID" id="1450828"/>
    <dbReference type="KEGG" id="mja:MJ_ECS01"/>
    <dbReference type="eggNOG" id="arCOG11560">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1088283_0_0_2"/>
    <dbReference type="OMA" id="KCHREYL"/>
    <dbReference type="OrthoDB" id="138336at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107483" description="Uncharacterized protein MJECS01">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <sequence length="276" mass="32558" checksum="2CA3E1D51756BAFE" modified="1997-11-01" version="1">MKFIFEKYYRHPINKYNGSKITPISQKNIFVVIKVELDDNELNNIHDEAKKLAEDVCEKNPSGDIRTEERKLLSSFCGLIAEKVVKQLITEKLMLIKDYIEIKGANVSNENFNYESHNDIEIHLSNDEIITIEIRSSIATKYDLNDILNKDFDILGSYTTSYKSKEHNKDYYFRIIFHNPGDYGYNPHLKKWEYNKRLENAFKQEKNKALDYFKNNVKVYFVGGCTFDDLENYGEHDDLKQKNAEYWIIRPITKGRDVLEILEILEKDILNKLGFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q60304</accession>
    <name>Y3405_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS05</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS05</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64516">
        <property type="entry name" value="E64516"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890098.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60304"/>
    <dbReference type="EnsemblBacteria" id="AAC37063">
        <property type="protein sequence ID" value="AAC37063"/>
        <property type="gene ID" value="MJ_ECS05"/>
    </dbReference>
    <dbReference type="GeneID" id="1450832"/>
    <dbReference type="KEGG" id="mja:MJ_ECS05"/>
    <dbReference type="eggNOG" id="arCOG12721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1792136_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107486" description="Uncharacterized protein MJECS05">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="144" mass="16357" checksum="24E5A15EDE7F451A" modified="1997-11-01" version="1">MESKEYRKLEYNYKAFLIFSKVAMLTFLTVGIGAIFTPQTYPIMPTIGFIVVAGIVSLIGMTIGALIIHQQYETLPANEKLEFKQKLLPEAYYICIELFGYGSLVLLYNTFTSNNPTLCVMSLLMAGLFILVVLVIWYFGYKSY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q60320</accession>
    <name>Y009_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0009</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0009</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64301">
        <property type="entry name" value="A64301"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869502.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60320"/>
    <dbReference type="STRING" id="243232.MJ_0009"/>
    <dbReference type="EnsemblBacteria" id="AAB97998">
        <property type="protein sequence ID" value="AAB97998"/>
        <property type="gene ID" value="MJ_0009"/>
    </dbReference>
    <dbReference type="GeneID" id="1450848"/>
    <dbReference type="KEGG" id="mja:MJ_0009"/>
    <dbReference type="eggNOG" id="arCOG05016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1040553_0_0_2"/>
    <dbReference type="OMA" id="FEPKLWN"/>
    <dbReference type="OrthoDB" id="52801at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106650" description="Uncharacterized protein MJ0009">
        <location>
            <begin position="1"/>
            <end position="276"/>
        </location>
    </feature>
    <sequence length="276" mass="32241" checksum="D974214C75F1A39A" modified="1997-11-01" version="1">MIFNENTPNFIDFKESFKELPLSDETFKIIEENGIKLREIAIGEFSGRDSVAAIIKAIEEGIDFVLPVVAFTGTDYGNINIFYKNWEIVNKRIKEIDKDKILLPLHFMFEPKLWNALNGRWVVLSFKRYGYYRPCIGCHAYLRIIRIPLAKHLGGKIISGERLYHNGDFKIDQIEEVLNVYSKICRDFDVELILPIRYIREGKKIKEIIGEEWEQGEKQFSCVFSGNYRDKDGKVIFDKEGILKMLNEFIYPASVEILKEGYKGNFNYLNIVKKLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q60321</accession>
    <name>Y013_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0013</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0013</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64301">
        <property type="entry name" value="E64301"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60321"/>
    <dbReference type="SMR" id="Q60321"/>
    <dbReference type="STRING" id="243232.MJ_0013"/>
    <dbReference type="EnsemblBacteria" id="AAB98000">
        <property type="protein sequence ID" value="AAB98000"/>
        <property type="gene ID" value="MJ_0013"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0013"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1631660_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q60321"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106653" description="Uncharacterized protein MJ0013">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <sequence length="162" mass="19588" checksum="8C53C3D9F88AFFBB" modified="1997-11-01" version="1">MPKKKNKLPTEIVLTYKVKHNHDLKNLPDEFIKISQRAIDIIWENINWKEKVVKHRYKIGKKKYKYYTTTRLIPKIPKDNDFKRELRNRLLEGWEFASHYVDGAIKTAYSAIESWKSNYLNVNRKKNKPIFKRPFVRVKTTLMKYDRKNGIIRITIKPRKSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q60339</accession>
    <name>FRHD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Coenzyme F420 hydrogenase subunit delta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Putative coenzyme F420 hydrogenase-processing subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">frhD</name>
        <name type="ordered locus">MJ0030</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64303">
        <property type="entry name" value="F64303"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60339"/>
    <dbReference type="SMR" id="Q60339"/>
    <dbReference type="STRING" id="243232.MJ_0030"/>
    <dbReference type="EnsemblBacteria" id="AAB98009">
        <property type="protein sequence ID" value="AAB98009"/>
        <property type="gene ID" value="MJ_0030"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0030"/>
    <dbReference type="eggNOG" id="arCOG04429">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099037_0_2_2"/>
    <dbReference type="InParanoid" id="Q60339"/>
    <dbReference type="OMA" id="CQKKYVS"/>
    <dbReference type="PhylomeDB" id="Q60339"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036211">
        <property type="term" value="P:protein modification process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06064">
        <property type="entry name" value="H2MP_F420-Reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="PTHR30302"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="SSF53163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0064">Aspartyl protease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000201946" description="Coenzyme F420 hydrogenase subunit delta">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="183" mass="20628" checksum="DD79B2FB9DEFE5A7" modified="1997-11-01" version="1">MNETMKAEIESQDDELFDLTPSYLKKEIMVLACGNILFADDGFSVHVIEKLNKILTDKEKQKIALVDAGAGAPQQVLTLIDENSKTKKIIVVDVIDWGIKPGEIKIIEKDELPNPKYHRLDSHDWPLAPLLREVAEKYNIEVKVVGCQAKYISEPDVYIGLSEEVEKAVDKAVEIILRELRGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>P54027</accession>
    <name>RL44E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L44e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl44e</name>
        <name type="ordered locus">MJ0249</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64331">
        <property type="entry name" value="B64331"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869747.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54027"/>
    <dbReference type="SMR" id="P54027"/>
    <dbReference type="STRING" id="243232.MJ_0249"/>
    <dbReference type="EnsemblBacteria" id="AAB98236">
        <property type="protein sequence ID" value="AAB98236"/>
        <property type="gene ID" value="MJ_0249"/>
    </dbReference>
    <dbReference type="GeneID" id="1451103"/>
    <dbReference type="KEGG" id="mja:MJ_0249"/>
    <dbReference type="eggNOG" id="arCOG04109">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114645_3_0_2"/>
    <dbReference type="InParanoid" id="P54027"/>
    <dbReference type="OMA" id="CKKHTIH"/>
    <dbReference type="OrthoDB" id="106603at2157"/>
    <dbReference type="PhylomeDB" id="P54027"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_L44e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="PTHR10369"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="SSF57829"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000149151" description="50S ribosomal protein L44e">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="1">
        <location>
            <begin position="11"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <sequence length="94" mass="11029" checksum="57C5EC30ACC13BD7" modified="1996-10-01" version="1">MKIPKKVRRYCPYCKKHTIHIVEKAKKGKPSELTWGQRQFRRVTAGYGGFPRPLPDRSKPVKKIDLRFKCTECGKMHTKANGCFRSGRFEFVEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>P54042</accession>
    <name>RL6_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl6</name>
        <name type="ordered locus">MJ0471</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64358">
        <property type="entry name" value="G64358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869972.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54042"/>
    <dbReference type="SMR" id="P54042"/>
    <dbReference type="STRING" id="243232.MJ_0471"/>
    <dbReference type="EnsemblBacteria" id="AAB98460">
        <property type="protein sequence ID" value="AAB98460"/>
        <property type="gene ID" value="MJ_0471"/>
    </dbReference>
    <dbReference type="GeneID" id="1451333"/>
    <dbReference type="KEGG" id="mja:MJ_0471"/>
    <dbReference type="eggNOG" id="arCOG04090">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065464_0_0_2"/>
    <dbReference type="InParanoid" id="P54042"/>
    <dbReference type="OMA" id="VFQDGIY"/>
    <dbReference type="OrthoDB" id="80629at2157"/>
    <dbReference type="PhylomeDB" id="P54042"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_L6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_L6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_L6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_L6_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_L6_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_L6P_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="PTHR11655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="SSF56053"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000131085" description="50S ribosomal protein L6">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <sequence length="182" mass="20655" checksum="3AB894EDB2AC18E3" modified="1996-10-01" version="1">MPVAAYIEERVKIPENVQVEINNNEVVVKSGGKELRRRFEHPKIVIKKEGDEIVIFCEYPRRKDKAMVGTIRAHINNMIKGVTEGFTYKLKIRYAHFPMKVSVKGNEVIIENFLGEKHPRRARIMEGVTVKISGEDVIVTGIDKEKVGQTAANIEQATRIKGRDPRVFQDGIYIVEKAGKAI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>P58018</accession>
    <name>YD5A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1351.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1351.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="unpublished observations" date="2001-04">
            <authorList>
                <person name="Medigue C."/>
                <person name="Bocs S."/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496805.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P58018"/>
    <dbReference type="SMR" id="P58018"/>
    <dbReference type="GeneID" id="27929997"/>
    <dbReference type="InParanoid" id="P58018"/>
    <dbReference type="OMA" id="FKGVGFP"/>
    <dbReference type="OrthoDB" id="55229at2157"/>
    <dbReference type="PhylomeDB" id="P58018"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012375">
        <property type="entry name" value="Glu_synth_lsu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018774">
        <property type="entry name" value="GOGAT_lg_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000107290" description="Uncharacterized protein MJ1351.1">
        <location>
            <begin position="2"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00609"/>
        </source>
    </evidence>
    <sequence length="362" mass="41487" checksum="6153A96F3D569739" modified="2006-12-12" version="2">MCGIIGFMSRKKRMIKGDKIALALDSLKERGNGKGSGYVGYGIYPTKYKDCYAFHILIDNTPKFEKIKVEVENVLEQYGTIVKDEEIPTEDGIIEKTQIPWRYFYEVDEKFADREEDVVVDIVMEINDKIDGAFVISSGKDLGVFKAVGWPDEVAKFYRIDKYEGYMWLAHARYPTNTRAWWGGAHPFNLLNWSVVHNGEITSYGTNKRFVEMFGYKCRLLTDTEVVAYILDLLMRKHKIPVEYALSALAPRFWDEIDKMPEEERELHTAIRLAYGGAMLNGPFAIAVGTPQGLIFMNGDIEKDTTMFGLTDRIKLRPLIAAEKDDMIFISSEESAIRRICPDLDRVWMPDAGMPVIARPWK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>P81231</accession>
    <name>Y78B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0785.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0785.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0795.1 and MJ1249.1.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870292.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81231"/>
    <dbReference type="STRING" id="243232.MJ_0785.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98790">
        <property type="protein sequence ID" value="AAB98790"/>
        <property type="gene ID" value="MJ_0785.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451664"/>
    <dbReference type="KEGG" id="mja:MJ_0785.1"/>
    <dbReference type="HOGENOM" id="CLU_1431651_0_0_2"/>
    <dbReference type="OrthoDB" id="137989at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107035" description="Uncharacterized protein MJ0785.1">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="189" mass="21527" checksum="7DB9DB3E8E954EDD" modified="1998-07-15" version="1">MENNKCGTMRGESVYLAYPFILGSVIFVEFFIFGLVYLTFGLGLKTIIITSGVILICLLPISIILIRLFIKSIAGKNKGKLIKKYTKLKILNKKYKILKVLLFIVGINFYLFGNLVSLNINPYTKFVLYSISAFFIIISIVVGDVIVEFYENGVFINPLGFYKWGEVGKEDLNDRLILKIDKLVIECKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>P81293</accession>
    <name>Y51B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized polyferredoxin-like protein MJ0514.2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0514.2</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81293"/>
    <dbReference type="STRING" id="243232.MJ_0514.2"/>
    <dbReference type="EnsemblBacteria" id="AAB98509">
        <property type="protein sequence ID" value="AAB98509"/>
        <property type="gene ID" value="MJ_0514.2"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0514.2"/>
    <dbReference type="eggNOG" id="arCOG02183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061721_0_0_2"/>
    <dbReference type="InParanoid" id="P81293"/>
    <dbReference type="OMA" id="IWYLYEF"/>
    <dbReference type="PhylomeDB" id="P81293"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159150" description="Uncharacterized polyferredoxin-like protein MJ0514.2">
        <location>
            <begin position="1"/>
            <end position="408"/>
        </location>
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    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
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            <end position="72"/>
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    </feature>
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            <begin position="151"/>
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    </feature>
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            <begin position="212"/>
            <end position="241"/>
        </location>
    </feature>
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            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 7" evidence="2">
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            <begin position="273"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 8" evidence="2">
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            <begin position="304"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
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            <position position="55"/>
        </location>
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        </ligand>
    </feature>
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        </ligand>
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        </ligand>
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            <name>[4Fe-4S] cluster</name>
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        </ligand>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
    </feature>
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        <location>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
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        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="408" mass="46462" checksum="2D3C2C10D5ECCD1D" modified="1998-06-01" version="1">MIVMASSLWYLYEFARKKWIKRFIDAKSDKSSYIPPERYRKIPPIVKFPEKCISCEGCKESCPAFAIEMIYNEEYNKKLPVIDEGSCVACANCIEVCPTGVLEMDKHRVETEGLFFDKPKYSNLIIDEEVCVRCGNCERACPINVIERKEGKYVINMALCISCKECIKVCPIENAIVVVDEKTLKEKIDKAFEIKNKKITGKLEIKENVIEKIPHIVSGLCVSCGICKDVCVGEIDLNEKKVVECVKCGLCIEVCSTTAIRIYKPIIPKRKDICYVIDEDLCIGCRICQKVCGSGAIKISKETKLPYIVPELCVRGGACARECPVGAIKVVKPEEAEEAVKVRIIEDKIIESIEKDLVLYTEKYGKVKEEIEKLSLKKLKEELKRRVYEENKRIMEKKRELYDKGNNS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>P81304</accession>
    <name>Y22A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0226.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0226.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869723.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81304"/>
    <dbReference type="EnsemblBacteria" id="AAB98217">
        <property type="protein sequence ID" value="AAB98217"/>
        <property type="gene ID" value="MJ_0226.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451078"/>
    <dbReference type="KEGG" id="mja:MJ_0226.1"/>
    <dbReference type="eggNOG" id="arCOG10447">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1405987_0_0_2"/>
    <dbReference type="OMA" id="WHYITST"/>
    <dbReference type="OrthoDB" id="95394at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106748" description="Uncharacterized protein MJ0226.1">
        <location>
            <begin position="1"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="193" mass="22933" checksum="C981628CFF211079" modified="1998-07-15" version="1">MDIELILLIVVLFLTPYLIALFIIFNPPYCILDYLLYKKYRKAKEEWHYITSTNMGMNRSRWIFILIVEIIALCSGFYILININRPHDEILTFSLIFLFIAIIYDKLTPASGTVEIYKEGIAVYIKIFNTLKPFLNRYIVLPWKFFKGYKIKSKNNTKYVILVPKSRLFFSIYLIDRDGNVEKTIRNHLNPIQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>P81331</accession>
    <name>YE8A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1489.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1489.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81331"/>
    <dbReference type="SMR" id="P81331"/>
    <dbReference type="STRING" id="243232.MJ_1489.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99513">
        <property type="protein sequence ID" value="AAB99513"/>
        <property type="gene ID" value="MJ_1489.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1489.1"/>
    <dbReference type="eggNOG" id="arCOG12721">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1840609_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107373" description="Uncharacterized protein MJ1489.1">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="139" mass="16198" checksum="D6A96C7CB3989A13" modified="1998-07-15" version="1">MLIAIGIMFQPLQYPTIPCIIFMVIVGLGLLFAFQFILGYGFEMHRQITIKDRIAFRNYVVGKIFNVLVEHSYYGLLLSTFNLFVYKKAITIRLCFIFAISIVIFWILGGKLIKKSLKLCFSWFYFFINPSISHKCNSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q57569</accession>
    <name>Y105_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0105</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0105</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64313">
        <property type="entry name" value="A64313"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57569"/>
    <dbReference type="EnsemblBacteria" id="AAB98085">
        <property type="protein sequence ID" value="AAB98085"/>
        <property type="gene ID" value="MJ_0105"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0105"/>
    <dbReference type="eggNOG" id="arCOG04420">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144580_0_0_2"/>
    <dbReference type="InParanoid" id="Q57569"/>
    <dbReference type="OMA" id="IPWEESD"/>
    <dbReference type="PhylomeDB" id="Q57569"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007417">
        <property type="entry name" value="DUF473"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04322">
        <property type="entry name" value="DUF473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106694" description="Uncharacterized protein MJ0105">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <sequence length="152" mass="17506" checksum="CB08D268DAA85897" modified="1997-11-01" version="1">MDTTRPLSWDFQSQLMSNLKILYCLNMLKLSNWYFGGIMKVYGLFGINENAINDFIENHIKTFTIINALNLETVKNLKEGDLVFITSTLREDLRNGTEGILGRVINVSLVPQMINGFEEKEIIAGRVQLEMLGFAKCVKYESIHVEITFRMY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57571</accession>
    <name>Y107_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0107</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0107</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="Nat. Struct. Biol." volume="6" first="750" last="754">
            <title>Identifying two ancient enzymes in Archaea using predicted secondary structure alignment.</title>
            <authorList>
                <person name="Xu H."/>
                <person name="Aurora R."/>
                <person name="Rose G.D."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="10426953"/>
            <dbReference type="DOI" id="10.1038/11525"/>
        </citation>
        <scope>LACK OF DHPS ACTIVITY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Unknown. Does not possess dihydropteroate synthase (DHPS) activity since it does not catalyze the condensation of 6-hydroxymethyl-7,8-dihydropterin pyrophosphate (DHPP) and 4-aminobenzoate to form 7,8-dihydropteroate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64313">
        <property type="entry name" value="C64313"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57571"/>
    <dbReference type="SMR" id="Q57571"/>
    <dbReference type="STRING" id="243232.MJ_0107"/>
    <dbReference type="EnsemblBacteria" id="AAB98089">
        <property type="protein sequence ID" value="AAB98089"/>
        <property type="gene ID" value="MJ_0107"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0107"/>
    <dbReference type="eggNOG" id="arCOG01978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041129_0_0_2"/>
    <dbReference type="InParanoid" id="Q57571"/>
    <dbReference type="OMA" id="DMIDLGM"/>
    <dbReference type="PhylomeDB" id="Q57571"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006760">
        <property type="term" value="P:folic acid-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006156">
        <property type="entry name" value="Dihydroneopterin_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005236">
        <property type="entry name" value="Dihydropt_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025595">
        <property type="entry name" value="DUF4346"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045406">
        <property type="entry name" value="DUF6513"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42844">
        <property type="entry name" value="PTHR42844"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14251">
        <property type="entry name" value="DUF4346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20123">
        <property type="entry name" value="DUF6513"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="SSF51717"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00284">
        <property type="entry name" value="TIGR00284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106696" description="Uncharacterized protein MJ0107">
        <location>
            <begin position="1"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Pterin-binding" evidence="2">
        <location>
            <begin position="146"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="525" mass="59645" checksum="AA8BA61C6B81863D" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q57580</accession>
    <name>ARFB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase</fullName>
            <shortName>FAPy deformylase</shortName>
            <ecNumber>3.5.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Formamide hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">arfB</name>
        <name type="ordered locus">MJ0116</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2009" name="Biochemistry" volume="48" first="4181" last="4188">
            <title>An iron(II) dependent formamide hydrolase catalyzes the second step in the archaeal biosynthetic pathway to riboflavin and 7,8-didemethyl-8-hydroxy-5-deazariboflavin.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="19309161"/>
            <dbReference type="DOI" id="10.1021/bi802341p"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>PATHWAY</scope>
        <scope>GENE NAME</scope>
        <scope>PH DEPENDENCE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of the formamide of 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-monophosphate (FAPy) to form 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (APy).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + H2O = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27282"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="3.5.1.102"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="2">Requires one Fe(2+) ion for activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="2">Requires an additional second metal ion that could be Fe(2+) or Zn(2+).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 7-8. Has approximately 80% maximal activity at pH 6.5 and 40% activity at pH 8.5.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the creatininase superfamily. FAPy deformylase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.102"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64314">
        <property type="entry name" value="D64314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57580"/>
    <dbReference type="SMR" id="Q57580"/>
    <dbReference type="STRING" id="243232.MJ_0116"/>
    <dbReference type="EnsemblBacteria" id="AAB98097">
        <property type="protein sequence ID" value="AAB98097"/>
        <property type="gene ID" value="MJ_0116"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0116"/>
    <dbReference type="eggNOG" id="arCOG04536">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1192640_0_0_2"/>
    <dbReference type="InParanoid" id="Q57580"/>
    <dbReference type="OMA" id="FLIINCH"/>
    <dbReference type="PhylomeDB" id="Q57580"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-15290"/>
    <dbReference type="BRENDA" id="3.5.1.102">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043729">
        <property type="term" value="F:2-amino-5-formylamino-6-(5-phosphoribosylamino)pyrimidin-4(3H)-one formate-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016811">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02116">
        <property type="entry name" value="FAPy_deform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024901">
        <property type="entry name" value="FAPy_deformylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="PTHR35005"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="SSF102215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000106700" description="2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="19309161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="225" mass="25305" checksum="929EF2CEC84DE35C" modified="1997-11-01" version="1">MQLRLSSGNVLNEKVHKVGIIALGSFLENHGAVLPIDTDIKIASYIALKASILTGAKFLGVVIPSTEYEYVKHGIHNKPEEVYSYMRFLINEGKKIGVEKFLIVNCHGGNILVESFLKDLEYEFDIKVEMINITFTHASTEEVSVGYIIGIAKADEETLKEHNNFEKYPEVGMVGLKEARENNKAIDKEAKVVKRFGVKLDKKLGEKILNNAIEKVVEKIKEMIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57598</accession>
    <name>TRMI_METJA</name>
    <protein>
        <recommendedName>
            <fullName>tRNA (adenine(57)-N(1)/adenine(58)-N(1))-methyltransferase TrmI</fullName>
            <ecNumber>2.1.1.219</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>tRNA(m1A57/58)-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">trmI</name>
        <name type="ordered locus">MJ0134</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 58 (m1A58) in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosine(57)/adenosine(58) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) + N(1)-methyladenosine(57)/N(1)-methyladenosine(58) in tRNA + 2 S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:41740"/>
            <dbReference type="Rhea" id="RHEA-COMP:9580"/>
            <dbReference type="Rhea" id="RHEA-COMP:9582"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74491"/>
            <dbReference type="EC" id="2.1.1.219"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer composed of a dimer of dimers.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains a large catalytic C-terminal domain that binds S-adenosyl-L-methionine and a smaller N-terminal domain that may play a role in tRNA recognition. Domains are connected by a linker region.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.219"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64316">
        <property type="entry name" value="F64316"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869627.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57598"/>
    <dbReference type="SMR" id="Q57598"/>
    <dbReference type="STRING" id="243232.MJ_0134"/>
    <dbReference type="EnsemblBacteria" id="AAB98115">
        <property type="protein sequence ID" value="AAB98115"/>
        <property type="gene ID" value="MJ_0134"/>
    </dbReference>
    <dbReference type="GeneID" id="1450975"/>
    <dbReference type="KEGG" id="mja:MJ_0134"/>
    <dbReference type="eggNOG" id="arCOG00978">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025402_0_1_2"/>
    <dbReference type="InParanoid" id="Q57598"/>
    <dbReference type="OMA" id="VVYPKDA"/>
    <dbReference type="OrthoDB" id="64860at2157"/>
    <dbReference type="PhylomeDB" id="Q57598"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016429">
        <property type="term" value="F:tRNA (adenine-N1-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="PTHR12133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000106712" description="tRNA (adenine(57)-N(1)/adenine(58)-N(1))-methyltransferase TrmI">
        <location>
            <begin position="1"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="98"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8GBB2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00952"/>
        </source>
    </evidence>
    <sequence length="282" mass="32207" checksum="558FB972B21CFA9C" modified="1997-11-01" version="1">MFAYKLLVDERGKRYLLKKNVEKFGTDLGIVDMKDIEEGVELKSHKGHTFYLVEPTMFDILKRMKRTVTTLLPKDIGFIIARAGIREGETVVEAGTGSGALTMYLSNAVGKTGKVITYDIRPEFAKVARKNLLRVGAIKKGQKIIGLDEEFDDEDEIEIEDGLFNVIQKIGDVREKIDEKDVDVIVLDLPDPWNVVENAKKALNKKRGRIVTYLPYIEQVKKTVEKLKEEGFWDIHTYEIIEREIEISEKGVRPSTRMIGHTGYITVARVPPEPLDREEEKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="61" xmlns="http://uniprot.org/uniprot">
    <accession>Q57642</accession>
    <name>Y183_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0183</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0183</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64322">
        <property type="entry name" value="H64322"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57642"/>
    <dbReference type="SMR" id="Q57642"/>
    <dbReference type="STRING" id="243232.MJ_0183"/>
    <dbReference type="EnsemblBacteria" id="AAB98180">
        <property type="protein sequence ID" value="AAB98180"/>
        <property type="gene ID" value="MJ_0183"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0183"/>
    <dbReference type="HOGENOM" id="CLU_3147975_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106732" description="Uncharacterized protein MJ0183">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <sequence length="48" mass="5688" checksum="19B753A15C69F8FB" modified="1996-11-01" version="1">MLSPDMPLKNLDEYDRLGIKKKADAIARFIENRWDYLQKNNMIALYGN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q57645</accession>
    <name>ARGJ_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Glutamate N-acetyltransferase</fullName>
            <ecNumber>2.3.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Ornithine acetyltransferase</fullName>
            <shortName>OATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Ornithine transacetylase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName>Glutamate N-acetyltransferase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Glutamate N-acetyltransferase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="primary">argJ</name>
        <name type="ordered locus">MJ0186</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Eur. J. Biochem." volume="267" first="5217" last="5226">
            <title>Characterization and kinetic mechanism of mono- and bifunctional ornithine acetyltransferases from thermophilic microorganisms.</title>
            <authorList>
                <person name="Marc F."/>
                <person name="Weigel P."/>
                <person name="Legrain C."/>
                <person name="Almeras Y."/>
                <person name="Santrot M."/>
                <person name="Glansdorff N."/>
                <person name="Sakanyan V."/>
            </authorList>
            <dbReference type="PubMed" id="10931207"/>
            <dbReference type="DOI" id="10.1046/j.1432-1327.2000.01593.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 185-192</scope>
        <scope>FUNCTION AS AN OATASE</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>NCIMB 8224 / CCM 2186 / VKM B-718</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes only the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>L-glutamate + N(2)-acetyl-L-ornithine = L-ornithine + N-acetyl-L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Competitively inhibited by L-ornithine.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">9.6 mM for N-acetyl-L-ornithine</KM>
            <KM evidence="2">11.3 mM for L-glutamate</KM>
            <Vmax evidence="2">175.0 mmol/min/mg enzyme</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 8.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is above 95 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ArgJ family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64323">
        <property type="entry name" value="C64323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869681.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57645"/>
    <dbReference type="SMR" id="Q57645"/>
    <dbReference type="STRING" id="243232.MJ_0186"/>
    <dbReference type="MEROPS" id="T05.002"/>
    <dbReference type="DNASU" id="1451033"/>
    <dbReference type="EnsemblBacteria" id="AAB98166">
        <property type="protein sequence ID" value="AAB98166"/>
        <property type="gene ID" value="MJ_0186"/>
    </dbReference>
    <dbReference type="GeneID" id="1451033"/>
    <dbReference type="KEGG" id="mja:MJ_0186"/>
    <dbReference type="eggNOG" id="arCOG04413">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027172_1_0_2"/>
    <dbReference type="InParanoid" id="Q57645"/>
    <dbReference type="OMA" id="DYVHENS"/>
    <dbReference type="OrthoDB" id="29910at2157"/>
    <dbReference type="PhylomeDB" id="Q57645"/>
    <dbReference type="SABIO-RK" id="Q57645"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00111"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:acetyl-CoA:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="PTHR23100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="SSF56266"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000002273" description="Glutamate N-acetyltransferase alpha chain" evidence="1">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000002274" description="Glutamate N-acetyltransferase beta chain" evidence="1">
        <location>
            <begin position="185"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="397"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="108"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="1">
        <location>
            <position position="109"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="1">
        <location>
            <begin position="184"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10931207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="402" mass="43757" checksum="2D915AB01CC7A8D6" modified="1997-11-01" version="1">MRVIDGGVTAPKGFKANGYKEGKFGVAIIISEKDAVGAGTFTTNKVVAHPVVLSRELIKNRDKFRAIVANSGNANCFTKDGMEDAKEMQRLVAELFNINEDEVLVASTGVIGRKMDMNIIKDRINKVYNLIKEGNSSINAAKAIMTTDTKPKEIAVEFEVNGKTVRVGGIAKGAGMIAPNMLHATMLCFITTDIEIDKESLTNILQKVVDKTFNNISVDGDTSTNDTVFVLANGLSGVNYEECGEEFENALLYVCRELAKMIVKDGEGATKFMEVVVKGAKTEEDAVKASKAIVNSLLVKTAVFGGDPNWGRIVAAVGYSGADFNPEVVDVILSNYKDEVYLVKDGIPLADEGTEELKKAEEIMKSDEIKIVVDLKMGEFENVCYGCDLSYEYVRINAEYTT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q57647</accession>
    <name>Y188_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0188</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0188</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64323">
        <property type="entry name" value="E64323"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869683.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57647"/>
    <dbReference type="SMR" id="Q57647"/>
    <dbReference type="STRING" id="243232.MJ_0188"/>
    <dbReference type="PRIDE" id="Q57647"/>
    <dbReference type="EnsemblBacteria" id="AAB98168">
        <property type="protein sequence ID" value="AAB98168"/>
        <property type="gene ID" value="MJ_0188"/>
    </dbReference>
    <dbReference type="GeneID" id="1451035"/>
    <dbReference type="KEGG" id="mja:MJ_0188"/>
    <dbReference type="eggNOG" id="arCOG00623">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982154_0_0_2"/>
    <dbReference type="InParanoid" id="Q57647"/>
    <dbReference type="OMA" id="GRIMFRT"/>
    <dbReference type="OrthoDB" id="46801at2157"/>
    <dbReference type="PhylomeDB" id="Q57647"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB/Sulfiredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016427">
        <property type="entry name" value="UCP004699_CBS/ParB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004699">
        <property type="entry name" value="UCP004699_CBS_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="SSF110849"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000106736" description="Uncharacterized protein MJ0188">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="9"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="67"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <sequence length="265" mass="29851" checksum="B5D0CB9955F69F4A" modified="1996-11-01" version="1">MSVKVSEYMTKKVVTVSKDNTVKDVIKLLKETGHNSFPVVENGKLIGIVSVHDIVGKDDNEKVENVMTKRKDMVVTTPDANIMDVGRIMFRTGFSKLPVVDEENNLVGIISNMDVIRSQIEKTTPKKLENIIKTYKSLGYNLRVEKEEVDVNKLRPTQNKIHADELVGRMYELKKGLAEPIIAIKTKRGDYYILVDGHHRAVAAYKMGVPKLDAYVIYLDTDKKLGIEKTAEIMNLKSLEDVKIVDSDDENSVKVIKYNKNGVLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q57678</accession>
    <name>NUCS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Endonuclease NucS</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nucS</name>
        <name type="ordered locus">MJ0225</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cleaves both 3' and 5' ssDNA extremities of branched DNA structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NucS endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64328">
        <property type="entry name" value="B64328"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57678"/>
    <dbReference type="SMR" id="Q57678"/>
    <dbReference type="STRING" id="243232.MJ_0225"/>
    <dbReference type="EnsemblBacteria" id="AAB98210">
        <property type="protein sequence ID" value="AAB98210"/>
        <property type="gene ID" value="MJ_0225"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0225"/>
    <dbReference type="eggNOG" id="arCOG01304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069350_1_0_2"/>
    <dbReference type="InParanoid" id="Q57678"/>
    <dbReference type="OMA" id="VHTDEGQ"/>
    <dbReference type="PhylomeDB" id="Q57678"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000014">
        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00722">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002793">
        <property type="entry name" value="Endonuclease_NucS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38814">
        <property type="entry name" value="PTHR38814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01939">
        <property type="entry name" value="NucS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155691" description="Endonuclease NucS">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00722"/>
        </source>
    </evidence>
    <sequence length="263" mass="30984" checksum="35F9F59D4C2D7A6E" modified="1996-11-01" version="1">MMRLEKVFYLTNPTTKDLENFIDMYVFKYILILLARCKVFYEGRAKSQLEEGDRVIIIKPDGAFLIHKDKKREPVNWQPSGSSIIWEVEDNFFILKSIRRKPKEELKVVISEVYHACAFNCEDYEEINLRGSESEMAEMIFRNPDLIEEGFKPISREYQIPTGIVDILGKDKENKWVILELKRRRADLQAVSQLKRYVEYFKNKYGEDKVRGILVSPSLTTGAEKLLKEENLEFKRLNPPKGSKRDLKHNIKTKKTTVLDEWL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57687</accession>
    <name>Y235_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0235</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0235</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64329">
        <property type="entry name" value="D64329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869733.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57687"/>
    <dbReference type="SMR" id="Q57687"/>
    <dbReference type="STRING" id="243232.MJ_0235"/>
    <dbReference type="EnsemblBacteria" id="AAB98227">
        <property type="protein sequence ID" value="AAB98227"/>
        <property type="gene ID" value="MJ_0235"/>
    </dbReference>
    <dbReference type="GeneID" id="1451088"/>
    <dbReference type="KEGG" id="mja:MJ_0235"/>
    <dbReference type="eggNOG" id="arCOG05025">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_186521_0_0_2"/>
    <dbReference type="OMA" id="EMEIMAS"/>
    <dbReference type="OrthoDB" id="131924at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106754" description="Uncharacterized protein MJ0235">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <sequence length="82" mass="9899" checksum="CB23BFE0DA7F4E9F" modified="1996-11-01" version="1">MEKLIINSMKKVFLEELKDLENELNQIFKKYNVKSKDELKKKIANGEIKEEQIKDDLERIEFLEENIERVMKCLREINVKSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57688</accession>
    <name>THIDN_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Bifunctional thiamine biosynthesis protein ThiDN</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName>Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase</fullName>
                <ecNumber evidence="2">2.7.1.49</ecNumber>
                <ecNumber evidence="2">2.7.4.7</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Hydroxymethylpyrimidine kinase</fullName>
                <shortName>HMP kinase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName>Hydroxymethylpyrimidine phosphate kinase</fullName>
                <shortName>HMP-P kinase</shortName>
                <shortName>HMP-phosphate kinase</shortName>
                <shortName>HMPP kinase</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>Thiamine-phosphate synthase ThiN</fullName>
                <shortName>TP synthase</shortName>
                <shortName>TPS</shortName>
                <ecNumber>2.5.1.3</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Thiamine-phosphate pyrophosphorylase</fullName>
                <shortName>TMP pyrophosphorylase</shortName>
                <shortName>TMP-PPase</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name type="primary">thiDN</name>
        <name type="ordered locus">MJ0236</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2009-02" db="PDB data bank">
            <title>Crystal structure of the C-terminal domain of protein MJ0236 (Y236_METJA).</title>
            <authorList>
                <consortium name="New York structural genomix research consortium (NYSGXRC)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.03 ANGSTROMS) OF 236-416</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P.</text>
    </comment>
    <comment type="function">
        <text evidence="3">Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-amino-5-hydroxymethyl-2-methylpyrimidine + ATP = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.49"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + ATP = 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + ADP</text>
            <dbReference type="Rhea" id="RHEA:19893"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62899"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate + 4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 2 H(+) = CO2 + diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:47848"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:62890"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>4-amino-2-methyl-5-(diphosphooxymethyl)pyrimidine + 4-methyl-5-(2-phosphooxyethyl)-thiazole + H(+) = diphosphate + thiamine phosphate</text>
            <dbReference type="Rhea" id="RHEA:22328"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:57841"/>
            <dbReference type="ChEBI" id="CHEBI:58296"/>
            <dbReference type="EC" id="2.5.1.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the ThiD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the ThiN family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98222" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.49" evidence="2"/>
    <dbReference type="EC" id="2.7.4.7" evidence="2"/>
    <dbReference type="EC" id="2.5.1.3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98222.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64329">
        <property type="entry name" value="E64329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496432.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PHP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.03 A"/>
        <property type="chains" value="A/B/D/E=236-416"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PHP"/>
    <dbReference type="AlphaFoldDB" id="Q57688"/>
    <dbReference type="SMR" id="Q57688"/>
    <dbReference type="STRING" id="243232.MJ_0236"/>
    <dbReference type="EnsemblBacteria" id="AAB98222">
        <property type="protein sequence ID" value="AAB98222"/>
        <property type="gene ID" value="MJ_0236"/>
    </dbReference>
    <dbReference type="GeneID" id="1451089"/>
    <dbReference type="KEGG" id="mja:MJ_0236"/>
    <dbReference type="eggNOG" id="arCOG00020">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035788_0_0_2"/>
    <dbReference type="InParanoid" id="Q57688"/>
    <dbReference type="OrthoDB" id="64383at2157"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00141"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57688"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004789">
        <property type="term" value="F:thiamine-phosphate diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045029">
        <property type="entry name" value="HMP/HMP-P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PTHR20858"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="SSF53639"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106755" description="Bifunctional thiamine biosynthesis protein ThiDN">
        <location>
            <begin position="1"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Thiamine-phosphate synthase">
        <location>
            <begin position="236"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>4-amino-5-hydroxymethyl-2-methylpyrimidine</name>
            <dbReference type="ChEBI" id="CHEBI:16892"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="240"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="269"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="279"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="282"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="289"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="295"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="303"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="309"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="327"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="335"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="342"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="347"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="352"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="361"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="378"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="391"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="399"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P76422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q9WZP7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="2PHP"/>
        </source>
    </evidence>
    <sequence length="417" mass="47056" checksum="810246AD46805E27" modified="2012-01-25" version="2">MVILAIGGYDPTSGAGISADIKTAHTLGVYCPTITTSVIPQNNKMVYEKFDLPEENIKNQFKAVFEEFDIEYVKTGVLTKPAIDTLLKYIDKYDLKVICDPVLASTTKFSFVDEKLMEKYIELFNKSFLITPNKEEYKKIMEFIKNNNLMIRNDLYILATGIDDILMKNFKPIKTFKGFRVDKEVHGTGCVYSTAITAFLSKGYDLEEAIKEAKRFVLSSVIYAKKSKFGYNSNPTYINKEKVIKNLSYAIYLLKKMNFTLIPEVGSNIAESLPFPKDFKDVAALTGRIIKNKLGGFYIVGDIEFGASEHIAKIILSASKFNPEIRACMNIKYDGGLIKLLKDKFAVSSFDRKEEPPNVSTMEWGTKIACEKFGGVPDIIYDRGGEGKEPMIRVLGRDAIEVVKKVEVIQKIYNTLM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57758</accession>
    <name>Y310_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0310</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0310</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64338">
        <property type="entry name" value="G64338"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869808.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57758"/>
    <dbReference type="SMR" id="Q57758"/>
    <dbReference type="STRING" id="243232.MJ_0310"/>
    <dbReference type="EnsemblBacteria" id="AAB98296">
        <property type="protein sequence ID" value="AAB98296"/>
        <property type="gene ID" value="MJ_0310"/>
    </dbReference>
    <dbReference type="GeneID" id="1451165"/>
    <dbReference type="KEGG" id="mja:MJ_0310"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118613_4_0_2"/>
    <dbReference type="InParanoid" id="Q57758"/>
    <dbReference type="OMA" id="MIRIEMK"/>
    <dbReference type="OrthoDB" id="103616at2157"/>
    <dbReference type="PhylomeDB" id="Q57758"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="PTHR13323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106786" description="Uncharacterized protein MJ0310">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <sequence length="114" mass="11872" checksum="55030A1155D57A5E" modified="1996-11-01" version="1">MIDRVLLELNKTEGIKGSMVVGKDGLVIASQLPGSVDAELVGAMASAAFGAAERTAAEIGMGTLEQTMIEGEHGKTLMVDAGEGILVVLTDAKVNLGLIRITMKRAADKIKAMF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57765</accession>
    <name>Y317_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0317</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0317</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64339">
        <property type="entry name" value="F64339"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869815.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57765"/>
    <dbReference type="STRING" id="243232.MJ_0317"/>
    <dbReference type="EnsemblBacteria" id="AAB98311">
        <property type="protein sequence ID" value="AAB98311"/>
        <property type="gene ID" value="MJ_0317"/>
    </dbReference>
    <dbReference type="GeneID" id="1451172"/>
    <dbReference type="KEGG" id="mja:MJ_0317"/>
    <dbReference type="eggNOG" id="arCOG05029">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083800_0_0_2"/>
    <dbReference type="OMA" id="RGVFSWT"/>
    <dbReference type="OrthoDB" id="52283at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106790" description="Uncharacterized protein MJ0317">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <sequence length="224" mass="26112" checksum="1EBF400D80C4AAC1" modified="1996-11-01" version="1">MKPKYALRKDMIGEFTLNKSFNTYRGKVLKADFNGPIEGIVMKNKKEHIYFYPLLALHMVKPLNCVPINVIPKTSLPTNPKNVHIKEALSRIVGRTLKVYYETPKTSYLGRLLGFTRGVFSWTLVLEIHGEVVLLFNPDYIVYYGTKWKFLKNNPPYKPPRLMNITKTANYLKRCLLEDVIIEPEYPRINIEDKVFVYPYGVVSKDDYLGKTVEDILKEKEFLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q57769</accession>
    <name>Y321_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0321</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0321</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64340">
        <property type="entry name" value="B64340"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869819.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57769"/>
    <dbReference type="STRING" id="243232.MJ_0321"/>
    <dbReference type="EnsemblBacteria" id="AAB98312">
        <property type="protein sequence ID" value="AAB98312"/>
        <property type="gene ID" value="MJ_0321"/>
    </dbReference>
    <dbReference type="GeneID" id="1451176"/>
    <dbReference type="KEGG" id="mja:MJ_0321"/>
    <dbReference type="eggNOG" id="arCOG10171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_158396_0_0_2"/>
    <dbReference type="OMA" id="GWINNKK"/>
    <dbReference type="OrthoDB" id="127280at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.810.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027387">
        <property type="entry name" value="Cytb/b6-like_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106792" description="Uncharacterized protein MJ0321">
        <location>
            <begin position="1"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="122" mass="13504" checksum="952EE0C8D020250B" modified="1996-11-01" version="1">MTNNDKIVAIVTSIAVICISLTVIFCDTLVLAVGVPTLVLLWLVFLGWINNKKLDKGEMRRAITGSIVIAFFIILIAISKNPDIYSNNKEIFSLFFGMVTTIIGYYFGYRSGKESKNSSGNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q57808</accession>
    <name>Y362_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0362</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0362</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64345">
        <property type="entry name" value="B64345"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869861.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57808"/>
    <dbReference type="STRING" id="243232.MJ_0362"/>
    <dbReference type="PRIDE" id="Q57808"/>
    <dbReference type="EnsemblBacteria" id="AAB98354">
        <property type="protein sequence ID" value="AAB98354"/>
        <property type="gene ID" value="MJ_0362"/>
    </dbReference>
    <dbReference type="GeneID" id="1451219"/>
    <dbReference type="KEGG" id="mja:MJ_0362"/>
    <dbReference type="eggNOG" id="arCOG03884">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_194995_0_0_2"/>
    <dbReference type="OrthoDB" id="126773at2157"/>
    <dbReference type="PhylomeDB" id="Q57808"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019205">
        <property type="entry name" value="DUF2080_transposon-encoded"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09853">
        <property type="entry name" value="DUF2080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106832" description="Uncharacterized protein MJ0362">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <sequence length="63" mass="7107" checksum="490E45E7CCAF260B" modified="1996-11-01" version="1">MKIRKKTWRLPKDATISFKGVVKPIGNSGMILIPKDYVGCKVLVTILDELGNEEFEIVETEKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q57831</accession>
    <name>CAS2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endoribonuclease Cas2</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas2</name>
        <name type="ordered locus">MJ0386</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Functions as a ssRNA-specific endoribonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer, forms a heterotetramer with a Cas1 homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated endoribonuclease Cas2 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64348">
        <property type="entry name" value="B64348"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57831"/>
    <dbReference type="SMR" id="Q57831"/>
    <dbReference type="STRING" id="243232.MJ_0386"/>
    <dbReference type="EnsemblBacteria" id="AAB98372">
        <property type="protein sequence ID" value="AAB98372"/>
        <property type="gene ID" value="MJ_0386"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0386"/>
    <dbReference type="eggNOG" id="arCOG04194">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_161124_0_1_2"/>
    <dbReference type="InParanoid" id="Q57831"/>
    <dbReference type="OMA" id="LTHVQNS"/>
    <dbReference type="PhylomeDB" id="Q57831"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043571">
        <property type="term" value="P:maintenance of CRISPR repeat elements"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09725">
        <property type="entry name" value="Cas2_I_II_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01471">
        <property type="entry name" value="Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021127">
        <property type="entry name" value="CRISPR_associated_Cas2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019199">
        <property type="entry name" value="Virulence_VapD/CRISPR_Cas2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34405">
        <property type="entry name" value="PTHR34405"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09827">
        <property type="entry name" value="CRISPR_Cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01573">
        <property type="entry name" value="cas2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106849" description="CRISPR-associated endoribonuclease Cas2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="114" mass="13655" checksum="2F817459771C9306" modified="1996-11-01" version="1">MLKKEWDYVNKILKKIKNIRNLLQDESMYVIIVYDVNVSRVNKIKSFLRKHLNWVQNSVFEGEVTKAEFERIKDGILRIIDEDEDSVIIYQFPLNFMPKREILGLEKNPIDDII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57858</accession>
    <name>Y415_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0415</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0415</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64351">
        <property type="entry name" value="G64351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869914.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57858"/>
    <dbReference type="SMR" id="Q57858"/>
    <dbReference type="STRING" id="243232.MJ_0415"/>
    <dbReference type="EnsemblBacteria" id="AAB98406">
        <property type="protein sequence ID" value="AAB98406"/>
        <property type="gene ID" value="MJ_0415"/>
    </dbReference>
    <dbReference type="GeneID" id="1451275"/>
    <dbReference type="KEGG" id="mja:MJ_0415"/>
    <dbReference type="eggNOG" id="arCOG06574">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656905_0_0_2"/>
    <dbReference type="OMA" id="QIICYYA"/>
    <dbReference type="OrthoDB" id="94626at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106861" description="Uncharacterized protein MJ0415">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="162" mass="18253" checksum="79D77FE542F10BFF" modified="1996-11-01" version="1">MKRLKLLGGVMLFAIVSLMVCGCMVVFPKKYPDVLYKEYDVIKIENRTINGVKTAIVYQVKTEIGARSSPYSLDADSKKDIGAITYYVFKNTDVDEVQIICYYAGGGGLQPYYKFKIKRRDAELSGLLNVSEKELPSATLYYIDKLISLGDLWINDRLPVNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q57864</accession>
    <name>Y421_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0421</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0421</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64352">
        <property type="entry name" value="E64352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869920.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57864"/>
    <dbReference type="SMR" id="Q57864"/>
    <dbReference type="STRING" id="243232.MJ_0421"/>
    <dbReference type="EnsemblBacteria" id="AAB98409">
        <property type="protein sequence ID" value="AAB98409"/>
        <property type="gene ID" value="MJ_0421"/>
    </dbReference>
    <dbReference type="GeneID" id="1451281"/>
    <dbReference type="KEGG" id="mja:MJ_0421"/>
    <dbReference type="eggNOG" id="arCOG00056">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_795999_0_0_2"/>
    <dbReference type="InParanoid" id="Q57864"/>
    <dbReference type="OMA" id="ESRYQIQ"/>
    <dbReference type="OrthoDB" id="27531at2157"/>
    <dbReference type="PhylomeDB" id="Q57864"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11718">
        <property type="entry name" value="THUMP_SPOUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041730">
        <property type="entry name" value="MJ0421-like_THUMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025849">
        <property type="entry name" value="SPOUT_MTase_fused_to_THUMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14419">
        <property type="entry name" value="SPOUT_MTase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106867" description="Uncharacterized protein MJ0421">
        <location>
            <begin position="1"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="THUMP" evidence="1">
        <location>
            <begin position="45"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <sequence length="360" mass="41397" checksum="6C268E2E09D2753B" modified="1996-11-01" version="1">MKFIIKTQKGFENIVVNNLKEIVDDFNYIVSPDGYQGIVIVESDEDIEDKILQIPEVERVLKVYFETETDFDKIVNLAEKIKDYIKEDETFAVETKKRGKHDFSSTDINIVLGAKIKDLTNASVDLNNPDKVVHVEVFKNKTYVSITPGEKFKKYTKEKRNARELFKKVVIVQMPYLGEKIVCKRFGEAIGRAAQGFEVKELIIAPKEKVDAYELMEFIKGVKIGQHSRYEIQKRAYPFEIKLVPVTVQDLYQVVRDKRRDNRLLIITDPKGDELSKIKDKLAYDLRKKREIIVFCGSREGIPRGLFRFADYIVDLAPHMTFATEHAIPAALIALWGVYSGEDLENSSKEEKTESNSNGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q57884</accession>
    <name>HYPB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable hydrogenase maturation factor HypB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">hypB</name>
        <name type="ordered locus">MJ0442</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference evidence="6 7" key="2">
        <citation type="journal article" date="2006" name="J. Biol. Chem." volume="281" first="27492" last="27502">
            <title>Structural insights into HypB, a GTP-binding protein that regulates metal binding.</title>
            <authorList>
                <person name="Gasper R."/>
                <person name="Scrima A."/>
                <person name="Wittinghofer A."/>
            </authorList>
            <dbReference type="PubMed" id="16807243"/>
            <dbReference type="DOI" id="10.1074/jbc.M600809200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH ZINC</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase. Exhibits a low intrinsic GTPase activity, which is essential for nickel insertion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SIMIBI class G3E GTPase family. HypB/HupM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64355">
        <property type="entry name" value="B64355"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869941.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2HF8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B=1-221"/>
    </dbReference>
    <dbReference type="PDB" id="2HF9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B=1-221"/>
    </dbReference>
    <dbReference type="PDBsum" id="2HF8"/>
    <dbReference type="PDBsum" id="2HF9"/>
    <dbReference type="AlphaFoldDB" id="Q57884"/>
    <dbReference type="SMR" id="Q57884"/>
    <dbReference type="STRING" id="243232.MJ_0442"/>
    <dbReference type="EnsemblBacteria" id="AAB98429">
        <property type="protein sequence ID" value="AAB98429"/>
        <property type="gene ID" value="MJ_0442"/>
    </dbReference>
    <dbReference type="GeneID" id="1451302"/>
    <dbReference type="KEGG" id="mja:MJ_0442"/>
    <dbReference type="eggNOG" id="arCOG01231">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056148_0_1_2"/>
    <dbReference type="InParanoid" id="Q57884"/>
    <dbReference type="OMA" id="ECHLDAH"/>
    <dbReference type="OrthoDB" id="76245at2157"/>
    <dbReference type="PhylomeDB" id="Q57884"/>
    <dbReference type="EvolutionaryTrace" id="Q57884"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
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            <name>Zn(2+)</name>
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        <location>
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        </location>
    </feature>
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    </feature>
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        <location>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="206"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O25560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P0AAN3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16807243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="16807243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="2HF8"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="7">
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    </evidence>
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        <source>
            <dbReference type="PDB" id="2HF9"/>
        </source>
    </evidence>
    <sequence length="221" mass="24340" checksum="0BB5415386A81138" modified="1996-11-01" version="1">MHLVGVLDIAKDILKANKRLADKNRKLLNKHGVVAFDFMGAIGSGKTLLIEKLIDNLKDKYKIACIAGDVIAKFDAERMEKHGAKVVPLNTGKECHLDAHLVGHALEDLNLDEIDLLFIENVGNLICPADFDLGTHKRIVVISTTEGDDTIEKHPGIMKTADLIVINKIDLADAVGADIKKMENDAKRINPDAEVVLLSLKTMEGFDKVLEFIEKSVKEVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q57921</accession>
    <name>Y498_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0498</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0498</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="AAB98486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="B64362"/>
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    <dbReference type="EnsemblBacteria" id="AAB98486">
        <property type="protein sequence ID" value="AAB98486"/>
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    </dbReference>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_132457_1_0_2"/>
    <dbReference type="InParanoid" id="Q57921"/>
    <dbReference type="OMA" id="ETCFGAY"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012025">
        <property type="entry name" value="UCP005642_methan"/>
    </dbReference>
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        <property type="entry name" value="DUF2102"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="UCP005642"/>
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    </dbReference>
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        <property type="entry name" value="methan_mark_6"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106900" description="Uncharacterized protein MJ0498">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
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    <sequence length="156" mass="17921" checksum="B514D56D41EAAAB7" modified="1996-11-01" version="1">MMKKTKVIVLAENALTTPGKLVRYINTLNQPVIVKETCFGAYIEGEEELVDKLAQEIRNYERNRIFCKDRGYAIWDKRRCRAFRGGGPREGFHQLEAEQAVLDKIGLALDKIDKEGIKPMEEVLAKENELIKRETKIPVEEFKNIIEKVLGSKNEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57930</accession>
    <name>TBP_METJA</name>
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        <recommendedName>
            <fullName>TATA-box-binding protein</fullName>
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        <alternativeName>
            <fullName>Box A-binding protein</fullName>
            <shortName>BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>TATA sequence-binding protein</fullName>
            <shortName>TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tbp</name>
        <name type="ordered locus">MJ0507</name>
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    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Genes Cells" volume="13" first="1127" last="1140">
            <title>Crystal structure of Methanococcus jannaschii TATA box-binding protein.</title>
            <authorList>
                <person name="Adachi N."/>
                <person name="Senda M."/>
                <person name="Natsume R."/>
                <person name="Senda T."/>
                <person name="Horikoshi M."/>
            </authorList>
            <dbReference type="PubMed" id="19090808"/>
            <dbReference type="DOI" id="10.1111/j.1365-2443.2008.01233.x"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 4-183</scope>
    </reference>
    <comment type="function">
        <text evidence="1">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBP family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB98497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="C64363"/>
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    <dbReference type="PDB" id="2Z8U">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B/P/Q=4-183"/>
    </dbReference>
    <dbReference type="PDBsum" id="2Z8U"/>
    <dbReference type="AlphaFoldDB" id="Q57930"/>
    <dbReference type="SMR" id="Q57930"/>
    <dbReference type="STRING" id="243232.MJ_0507"/>
    <dbReference type="PRIDE" id="Q57930"/>
    <dbReference type="EnsemblBacteria" id="AAB98497">
        <property type="protein sequence ID" value="AAB98497"/>
        <property type="gene ID" value="MJ_0507"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0507"/>
    <dbReference type="eggNOG" id="arCOG01764">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060161_4_3_2"/>
    <dbReference type="InParanoid" id="Q57930"/>
    <dbReference type="OMA" id="NCEYEPE"/>
    <dbReference type="PhylomeDB" id="Q57930"/>
    <dbReference type="EvolutionaryTrace" id="Q57930"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140223">
        <property type="term" value="F:general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="PTHR10126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000154008" description="TATA-box-binding protein">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="1">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="repeat" description="2">
        <location>
            <begin position="101"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="25"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="45"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="50"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="54"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="62"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="72"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="116"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="133"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="141"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="145"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="153"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2Z8U"/>
        </source>
    </evidence>
    <sequence length="183" mass="20233" checksum="3C648FA0FC404944" modified="1996-11-01" version="1">MKIMEPEIKIVNVVVSTKIGDNIDLEEVAMILENAEYEPEQFPGLVCRLSVPKVALLIFRSGKVNCTGAKSKEEAEIAIKKIIKELKDAGIDVIENPEIKIQNMVATADLGIEPNLDDIALMVEGTEYEPEQFPGLVYRLDDPKVVVLIFGSGKVVITGLKSEEDAKRALKKILDTIKEVQEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q57933</accession>
    <name>Y513_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0513</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0513</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64364">
        <property type="entry name" value="A64364"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57933"/>
    <dbReference type="STRING" id="243232.MJ_0513"/>
    <dbReference type="EnsemblBacteria" id="AAB98512">
        <property type="protein sequence ID" value="AAB98512"/>
        <property type="gene ID" value="MJ_0513"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0513"/>
    <dbReference type="eggNOG" id="arCOG08281">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1173375_0_0_2"/>
    <dbReference type="OMA" id="MFIRGFV"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106906" description="Uncharacterized protein MJ0513">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <sequence length="241" mass="27890" checksum="88C7D3ACE842AC57" modified="1996-11-01" version="1">MMIMVKYGIKIEDDRVEIVRYSTVYTDEGVEELEEIYLQIKADDYESILGIYEPYPKKDVRFVGNLDDLKVVKGQEMRTAVPIPLSLCRAKYLKRIDEDDERITYLDINGVPIQRGIIVGIVVGVQHKRTSTGKDYTIFRIFDGYGWGRLRLFGIKANPEIFTGMFIRGFVRFGAVEFRTEEGELRKAISLTLNDIPVIVQPKEYIVHKKFIDEVVLPRVAPELIEEDKEEEETDEETINS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q57938</accession>
    <name>Y518_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0518</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0518</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64364">
        <property type="entry name" value="F64364"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870021.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57938"/>
    <dbReference type="STRING" id="243232.MJ_0518"/>
    <dbReference type="EnsemblBacteria" id="AAB98507">
        <property type="protein sequence ID" value="AAB98507"/>
        <property type="gene ID" value="MJ_0518"/>
    </dbReference>
    <dbReference type="GeneID" id="1451382"/>
    <dbReference type="KEGG" id="mja:MJ_0518"/>
    <dbReference type="eggNOG" id="arCOG04834">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366257_0_0_2"/>
    <dbReference type="OMA" id="MNEVPFM"/>
    <dbReference type="OrthoDB" id="128796at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019211">
        <property type="entry name" value="EhaL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011305">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09877">
        <property type="entry name" value="DUF2104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004953">
        <property type="entry name" value="EhaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106908" description="Uncharacterized protein MJ0518">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="94" mass="10552" checksum="BD9002A28C1B2D73" modified="1996-11-01" version="1">MGLFELNLAIILFIIGNFIGLEYSYRKYSSPYVEKGIDKFALAISVFGGILINSPLYMLGCLLIGFPLGMRPGYGRVEFVVGLAVALFLYFLRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57979</accession>
    <name>SURE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="1">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">surE</name>
        <name type="ordered locus">MJ0559</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98553" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98553.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64369">
        <property type="entry name" value="G64369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496542.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57979"/>
    <dbReference type="SMR" id="Q57979"/>
    <dbReference type="STRING" id="243232.MJ_0559"/>
    <dbReference type="EnsemblBacteria" id="AAB98553">
        <property type="protein sequence ID" value="AAB98553"/>
        <property type="gene ID" value="MJ_0559"/>
    </dbReference>
    <dbReference type="GeneID" id="1451424"/>
    <dbReference type="KEGG" id="mja:MJ_0559"/>
    <dbReference type="eggNOG" id="arCOG02303">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_1_3_2"/>
    <dbReference type="InParanoid" id="Q57979"/>
    <dbReference type="OMA" id="DCVHIAL"/>
    <dbReference type="OrthoDB" id="110625at2157"/>
    <dbReference type="PhylomeDB" id="Q57979"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106411">
        <property type="term" value="F:XMP 5'-nucleosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="PTHR30457"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SSF64167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000111864" description="5'-nucleotidase SurE">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="266" mass="29771" checksum="A5D5D497F15D7281" modified="2000-05-30" version="2">MEILIVNDDGIYSPSLIALYNALKEKFSDANITIVAPTNQQSGIGRAISLFEPLRMTKVKLAKDIVGYAVSGTPTDCVILGIYQILKKVPDLVISGINIGENLGTEIMTSGTLGAAFEAAHHGAKSIASSLQITSDHLKFKELDIPINFEIPAKITAKIAEKYLDYDMPCDVLNINIPENATLETPIEITRLARKMYTTHVEERIDPRGRSYYWIDGYPIFEEEEDTDVYVLRKKRHISITPLTLDTTIKNLDEFKEKYGKILCEM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58007</accession>
    <name>Y587_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0587</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0587</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0129 and MJ0554.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64373">
        <property type="entry name" value="C64373"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870091.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58007"/>
    <dbReference type="SMR" id="Q58007"/>
    <dbReference type="STRING" id="243232.MJ_0587"/>
    <dbReference type="DNASU" id="1451452"/>
    <dbReference type="EnsemblBacteria" id="AAB98584">
        <property type="protein sequence ID" value="AAB98584"/>
        <property type="gene ID" value="MJ_0587"/>
    </dbReference>
    <dbReference type="GeneID" id="1451452"/>
    <dbReference type="KEGG" id="mja:MJ_0587"/>
    <dbReference type="HOGENOM" id="CLU_1718222_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58007"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035582">
        <property type="entry name" value="DUF5418"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17439">
        <property type="entry name" value="DUF5418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106946" description="Uncharacterized protein MJ0587">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="153" mass="17733" checksum="282C1DABE1827DDC" modified="1997-11-01" version="1">MVNEYKAHSSFILKVVITLIGYWIASILAIIIYSMFFKIETNTFLLCLLLPTPIIWFNILIGMGLTYRCMENLTIYDKHKLWCVFVRDLTLTILATILATLTTMELYQIEHPLKPIEFVFIVGLVLIVGFTIITTLIIKYLKIIKNLKKISKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58008</accession>
    <name>TRUD1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable tRNA pseudouridine synthase D 1</fullName>
            <ecNumber evidence="1">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA pseudouridylate synthase D 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA-uridine isomerase D 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">truD1</name>
        <name type="ordered locus">MJ0588</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98579" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98579.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64373">
        <property type="entry name" value="D64373"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58008"/>
    <dbReference type="SMR" id="Q58008"/>
    <dbReference type="STRING" id="243232.MJ_0588"/>
    <dbReference type="EnsemblBacteria" id="AAB98579">
        <property type="protein sequence ID" value="AAB98579"/>
        <property type="gene ID" value="MJ_0588"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0588"/>
    <dbReference type="eggNOG" id="arCOG04252">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005281_4_1_2"/>
    <dbReference type="InParanoid" id="Q58008"/>
    <dbReference type="OMA" id="GFPNYFD"/>
    <dbReference type="PhylomeDB" id="Q58008"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106029">
        <property type="term" value="F:tRNA pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="PTHR13326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="SSF55120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000152540" description="Probable tRNA pseudouridine synthase D 1">
        <location>
            <begin position="1"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="TRUD" evidence="1">
        <location>
            <begin position="167"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="392" mass="46320" checksum="DC00CC533E6E065C" modified="2003-09-26" version="2">MPLNMNKYLTDAYTGGIIKKYPEDFIVEEITPEGIILEVGKSIEFKDEENWKGNYIHFTLEKRNWTTLDAIREIANRVGKQRKHFGFAGNKDKYAVTTQRVGCFNVKLEDLMKVKIKGIILRDFQKTNRKIRLGDLWGNRFTIRVREPELKGKELEEALNKLCKLKYFLNYYGVQRFGTTRPITHIVGRFIIERDWEGAFHAYCGTPLPYDDKKSKLARELVDEENFKEAYKKFPKAFFYERRMIKAYIETGSYQKAFMILPPYLRCMFINAYQSYLFNEIINRRFEYGFEPMEGDILIDNVPSGALFGYKTRFASGIQGEIEREIYERENLSPEDFKIGEFGSFIGDRRAMIGKIYNMKYWIEDDSYVLQFCLKKGNYATSVLREFIEKKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q58026</accession>
    <name>Y609_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0609</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0609</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64376">
        <property type="entry name" value="A64376"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870113.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3GI8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.59 A"/>
        <property type="chains" value="C=1-435"/>
    </dbReference>
    <dbReference type="PDB" id="3GI9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.48 A"/>
        <property type="chains" value="C=1-435"/>
    </dbReference>
    <dbReference type="PDB" id="3GIA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.32 A"/>
        <property type="chains" value="A=1-435"/>
    </dbReference>
    <dbReference type="PDBsum" id="3GI8"/>
    <dbReference type="PDBsum" id="3GI9"/>
    <dbReference type="PDBsum" id="3GIA"/>
    <dbReference type="AlphaFoldDB" id="Q58026"/>
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    <dbReference type="TCDB" id="2.A.3.6.3">
        <property type="family name" value="the amino acid-polyamine-organocation (apc) family"/>
    </dbReference>
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        <property type="antibodies" value="1 sequenced antibody"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98602">
        <property type="protein sequence ID" value="AAB98602"/>
        <property type="gene ID" value="MJ_0609"/>
    </dbReference>
    <dbReference type="GeneID" id="1451474"/>
    <dbReference type="KEGG" id="mja:MJ_0609"/>
    <dbReference type="eggNOG" id="arCOG00009">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_15_2_2"/>
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    <dbReference type="OMA" id="HKAFGIH"/>
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    <dbReference type="EvolutionaryTrace" id="Q58026"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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    <keyword id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
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        <source>
            <dbReference type="PDB" id="3GI9"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3GIA"/>
        </source>
    </evidence>
    <sequence length="435" mass="47358" checksum="3F289E21FC56CF97" modified="1996-11-01" version="1">MELKNKKLSLWEAVSMAVGVMIGASIFSIFGVGAKIAGRNLPETFILSGIYALLVAYSYTKLGAKIVSNAGPIAFIHKAIGDNIITGALSILLWMSYVISIALFAKGFAGYFLPLINAPINTFNIAITEIGIVAFFTALNFFGSKAVGRAEFFIVLVKLLILGLFIFAGLITIHPSYVIPDLAPSAVSGMIFASAIFFLSYMGFGVITNASEHIENPKKNVPRAIFISILIVMFVYVGVAISAIGNLPIDELIKASENALAVAAKPFLGNLGFLLISIGALFSISSAMNATIYGGANVAYSLAKDGELPEFFERKVWFKSTEGLYITSALGVLFALLFNMEGVASITSAVFMVIYLFVILSHYILIDEVGGRKEIVIFSFIVVLGVFLLLLYYQWITNRFVFYGIIATFIGVLIFEIIYRKVTKRTFSNNMYVKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58032</accession>
    <name>VATD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>V-type ATP synthase subunit D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>V-ATPase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">atpD</name>
        <name type="ordered locus">MJ0615</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase D subunit family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="EnsemblBacteria" id="AAB98610">
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    </dbReference>
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    <dbReference type="KEGG" id="mja:MJ_0615"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033176">
        <property type="term" value="C:proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
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    </dbReference>
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        <property type="entry name" value="V_ATPase_subD"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144250" description="V-type ATP synthase subunit D">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="216" mass="24951" checksum="DBF27A987C56913A" modified="1996-11-01" version="1">MQRVNPTRMELLKLKNKIKLAEKGHKLLKQKRDALIMEFFQIIEQASDLRDKVEAKLAEAYKDLIMAQTVMGTLAVKEAALAAKNDKLEVDMDTKNIMGVTVPTFEIYNVRRKVGERGYSPYGVSSKLDEAAKKFEEALELITELAEIETSIKLLAEEIITTKRRVNALEYVIIPRLKSLKKYISMRLDEMERENFFRLKLIKSRIEKREAEGETV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58035</accession>
    <name>Y618_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0235 protein MJ0618</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0618</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0235 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64377">
        <property type="entry name" value="B64377"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870123.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58035"/>
    <dbReference type="SMR" id="Q58035"/>
    <dbReference type="STRING" id="243232.MJ_0618"/>
    <dbReference type="EnsemblBacteria" id="AAB98613">
        <property type="protein sequence ID" value="AAB98613"/>
        <property type="gene ID" value="MJ_0618"/>
    </dbReference>
    <dbReference type="GeneID" id="1451484"/>
    <dbReference type="KEGG" id="mja:MJ_0618"/>
    <dbReference type="eggNOG" id="arCOG04058">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130694_6_1_2"/>
    <dbReference type="InParanoid" id="Q58035"/>
    <dbReference type="OMA" id="IGKINEW"/>
    <dbReference type="OrthoDB" id="123783at2157"/>
    <dbReference type="PhylomeDB" id="Q58035"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00634">
        <property type="entry name" value="UPF0235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005228">
        <property type="entry name" value="CHP00251"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13420">
        <property type="entry name" value="PTHR13420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="SSF69786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00251">
        <property type="entry name" value="TIGR00251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139468" description="UPF0235 protein MJ0618">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00634"/>
        </source>
    </evidence>
    <sequence length="98" mass="11289" checksum="E01AAF935D15500A" modified="1996-11-01" version="1">MIEKIIKESREGVLIDIDVQANAKKNEIVGINEWRKRLSIKIKAPATEGKANKEIIKFFKEIFKKDVEIVSGKLNPQKTVLIGDIKKDEVIEILKRYL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58046</accession>
    <name>Y629_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0111 protein MJ0629</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0629</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0111 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64378">
        <property type="entry name" value="E64378"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58046"/>
    <dbReference type="SMR" id="Q58046"/>
    <dbReference type="STRING" id="243232.MJ_0629"/>
    <dbReference type="EnsemblBacteria" id="AAB98621">
        <property type="protein sequence ID" value="AAB98621"/>
        <property type="gene ID" value="MJ_0629"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0629"/>
    <dbReference type="eggNOG" id="arCOG02640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104916_1_1_2"/>
    <dbReference type="InParanoid" id="Q58046"/>
    <dbReference type="OMA" id="LPNMRRE"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002727">
        <property type="entry name" value="DUF47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018445">
        <property type="entry name" value="Put_Phosphate_transp_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36536">
        <property type="entry name" value="PTHR36536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01865">
        <property type="entry name" value="PhoU_div"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00153">
        <property type="entry name" value="TIGR00153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000154913" description="UPF0111 protein MJ0629">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="214" mass="25428" checksum="EB6895CCC79C4AD9" modified="1996-11-01" version="1">MDVITMSIFLFERDNEKSVIDNLRLLIQMSLKSIELLKDYMNSKDEKILKEIIKIEEEGDETTKNIRINLEKAFLPNMRRELSRSAELLDETLDSLKHAAMLYELLKEEFDEYLKNEIDLVLMITVDMFQHLDRVLDVIEKGGDLDPIIKEIKDKEKFIDDVYQNRIYKYLINLEVESFWEGKILCDFIDNIVNISDYIEDVADELHIIYLHTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58047</accession>
    <name>Y630_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative phosphate permease MJ0630</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0630</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Potential transporter for phosphate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64378">
        <property type="entry name" value="F64378"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870135.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58047"/>
    <dbReference type="SMR" id="Q58047"/>
    <dbReference type="STRING" id="243232.MJ_0630"/>
    <dbReference type="TCDB" id="2.A.20.3.1">
        <property type="family name" value="the inorganic phosphate transporter (pit) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98622">
        <property type="protein sequence ID" value="AAB98622"/>
        <property type="gene ID" value="MJ_0630"/>
    </dbReference>
    <dbReference type="GeneID" id="1451496"/>
    <dbReference type="KEGG" id="mja:MJ_0630"/>
    <dbReference type="eggNOG" id="arCOG02267">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015355_0_0_2"/>
    <dbReference type="InParanoid" id="Q58047"/>
    <dbReference type="OMA" id="RANPHKA"/>
    <dbReference type="OrthoDB" id="40216at2157"/>
    <dbReference type="PhylomeDB" id="Q58047"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001204">
        <property type="entry name" value="Phos_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11101">
        <property type="entry name" value="PTHR11101"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01384">
        <property type="entry name" value="PHO4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000080804" description="Putative phosphate permease MJ0630">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="297" mass="32036" checksum="4A51E12ADE68026C" modified="1996-11-01" version="1">MITIEISINLELIISFYLLFILGANNVANAIGTAYASRATTYRNLLILFSISVIIGSLFAKNVGSTVNSLSSDALTALIISALVMTLSTYKKVPISLHTVIICSLIGLNFNSSNLYVFGEILLSWILSPIIAVVIAYILYSAYEKIDISILKKITMIRYFLLISAAVVAFNLGSNDLPTVLGTFTTSQIIYIIGAIFLCLGAYLYGNRVSETLSMITNLSVSSAFIAQLSGGLAVTIFTALGMPVSTTQAIVGGILGVGLTKGIKTVRWKVLKNIIFWWVVAPIIALIIGFIINRMI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58050</accession>
    <name>SYL_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Leucine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Leucyl-tRNA synthetase</fullName>
            <shortName evidence="1">LeuRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">leuS</name>
        <name type="ordered locus">MJ0633</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64379">
        <property type="entry name" value="A64379"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58050"/>
    <dbReference type="SMR" id="Q58050"/>
    <dbReference type="STRING" id="243232.MJ_0633"/>
    <dbReference type="PRIDE" id="Q58050"/>
    <dbReference type="EnsemblBacteria" id="AAB98628">
        <property type="protein sequence ID" value="AAB98628"/>
        <property type="gene ID" value="MJ_0633"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0633"/>
    <dbReference type="eggNOG" id="arCOG00809">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004174_0_0_2"/>
    <dbReference type="InParanoid" id="Q58050"/>
    <dbReference type="OMA" id="AWNMAFQ"/>
    <dbReference type="PhylomeDB" id="Q58050"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00049_A">
        <property type="entry name" value="Leu_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020791">
        <property type="entry name" value="Leu-tRNA-lgase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004493">
        <property type="entry name" value="Leu-tRNA-synth_Ia_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45794">
        <property type="entry name" value="PTHR45794"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00395">
        <property type="entry name" value="leuS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000152131" description="Leucine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="633"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="636"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00049"/>
        </source>
    </evidence>
    <sequence length="942" mass="110184" checksum="25A551461566A399" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58068</accession>
    <name>Y652_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable RNA-binding protein MJ0652</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0652</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64381">
        <property type="entry name" value="D64381"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58068"/>
    <dbReference type="SMR" id="Q58068"/>
    <dbReference type="STRING" id="243232.MJ_0652"/>
    <dbReference type="EnsemblBacteria" id="AAB98643">
        <property type="protein sequence ID" value="AAB98643"/>
        <property type="gene ID" value="MJ_0652"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0652"/>
    <dbReference type="eggNOG" id="arCOG01346">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095994_2_1_2"/>
    <dbReference type="InParanoid" id="Q58068"/>
    <dbReference type="OMA" id="FKPREGW"/>
    <dbReference type="PhylomeDB" id="Q58068"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017924">
        <property type="entry name" value="RNA-binding_YhbY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="SSF75471"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00253">
        <property type="entry name" value="RNA_bind_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000202169" description="Probable RNA-binding protein MJ0652">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="CRM" evidence="1">
        <location>
            <begin position="11"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <sequence length="134" mass="15731" checksum="8183CEEB75B07A12" modified="1996-11-01" version="1">MVIFMTEEQKRKLTGKMKRMLRAKAHHLEPVVWVGKEGSEKVIKEVDRQLKERGLIKVKVRKAALLYEDKYEIAEKLAKACDAEVVSVVGHVITLFRPREGWKKYLAKKPKKKVKKDEKIIELFEKFKKKAVKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58094</accession>
    <name>TKTN_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative transketolase N-terminal section</fullName>
            <shortName>TK</shortName>
            <ecNumber>2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0681</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="1">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="1">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transketolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Could be the product of a pseudogene. Corresponds to the N-terminal of members of this family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64385">
        <property type="entry name" value="A64385"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870186.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58094"/>
    <dbReference type="SMR" id="Q58094"/>
    <dbReference type="STRING" id="243232.MJ_0681"/>
    <dbReference type="EnsemblBacteria" id="AAB98676">
        <property type="protein sequence ID" value="AAB98676"/>
        <property type="gene ID" value="MJ_0681"/>
    </dbReference>
    <dbReference type="GeneID" id="1451547"/>
    <dbReference type="KEGG" id="mja:MJ_0681"/>
    <dbReference type="eggNOG" id="arCOG01053">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_4_1_2"/>
    <dbReference type="InParanoid" id="Q58094"/>
    <dbReference type="OMA" id="MVHAVNS"/>
    <dbReference type="OrthoDB" id="73908at2157"/>
    <dbReference type="PhylomeDB" id="Q58094"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="uncertain"/>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000191914" description="Putative transketolase N-terminal section">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="274" mass="30336" checksum="FE3C053AC09F0A4E" modified="1996-11-01" version="1">MDNNLEIKDLEKIAKKVRYNIVKMVGLAKSGHPGGSLSATDIIVALYFKLMNYSPDNPYKKDRDRFVLSKGHAAPALYAVLSELGIIEEEELWKLRRLEGKLQGHPSMDTPGVEICTGSLGQGFSAAVGMALGCRLDKLNNYVYVLLGDGECQEGIVWEAAMAAAHYKLDNLIAFIDRNKLQIDGCTEDVMSLGDIKAKFEAFGWDVFEIDGHNFEEIINTVEKAKSMKNGKPKMIIAYTVKGKGVSFMENNVAFHGKAPNEEQLKQALEELSE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58116</accession>
    <name>HMDH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>3-hydroxy-3-methylglutaryl-coenzyme A reductase</fullName>
            <shortName>HMG-CoA reductase</shortName>
            <ecNumber>1.1.1.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">hmgA</name>
        <name type="ordered locus">MJ0705</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts HMG-CoA to mevalonate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(R)-mevalonate + CoA + 2 NADP(+) = (3S)-hydroxy-3-methylglutaryl-CoA + 2 H(+) + 2 NADPH</text>
            <dbReference type="Rhea" id="RHEA:15989"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HMG-CoA reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.34"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64388">
        <property type="entry name" value="A64388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870211.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58116"/>
    <dbReference type="SMR" id="Q58116"/>
    <dbReference type="STRING" id="243232.MJ_0705"/>
    <dbReference type="EnsemblBacteria" id="AAB98699">
        <property type="protein sequence ID" value="AAB98699"/>
        <property type="gene ID" value="MJ_0705"/>
    </dbReference>
    <dbReference type="GeneID" id="1451573"/>
    <dbReference type="KEGG" id="mja:MJ_0705"/>
    <dbReference type="eggNOG" id="arCOG04260">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001734_2_2_2"/>
    <dbReference type="InParanoid" id="Q58116"/>
    <dbReference type="OMA" id="DDKMTRA"/>
    <dbReference type="OrthoDB" id="26445at2157"/>
    <dbReference type="PhylomeDB" id="Q58116"/>
    <dbReference type="UniPathway" id="UPA00058">
        <property type="reaction ID" value="UER00103"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004420">
        <property type="term" value="F:hydroxymethylglutaryl-CoA reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015936">
        <property type="term" value="P:coenzyme A metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00643">
        <property type="entry name" value="HMG-CoA_reductase_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.770.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002202">
        <property type="entry name" value="HMG_CoA_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023074">
        <property type="entry name" value="HMG_CoA_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023076">
        <property type="entry name" value="HMG_CoA_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004554">
        <property type="entry name" value="HMG_CoA_Rdtase_eu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009023">
        <property type="entry name" value="HMG_CoA_Rdtase_NAD(P)-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009029">
        <property type="entry name" value="HMG_CoA_Rdtase_sub-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10572">
        <property type="entry name" value="PTHR10572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00368">
        <property type="entry name" value="HMG-CoA_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00071">
        <property type="entry name" value="HMGCOARDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55035">
        <property type="entry name" value="SSF55035"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56542">
        <property type="entry name" value="SSF56542"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00533">
        <property type="entry name" value="HMG_CoA_R_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00066">
        <property type="entry name" value="HMG_COA_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00318">
        <property type="entry name" value="HMG_COA_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50065">
        <property type="entry name" value="HMG_COA_REDUCTASE_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000114461" description="3-hydroxy-3-methylglutaryl-coenzyme A reductase">
        <location>
            <begin position="1"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="405" mass="44128" checksum="F8F8E4729A77DFC9" modified="1996-11-01" version="1">MENYNDILEKMLNGEIKPYQLDKMFGSKIATEIRRKFIEKKVGIEFKHICNYSIDEEMAMKKNIENMIGAIQIPLGFAGPLKINGEYAKGEFYIPLATTEGALVASVNRGCSIITKCGGATVRVIDDKMTRAPCLKTKSVVDAIKVRDWIRENFERIKEVAESTTRHGKLIKIEPILIVGRNLYPRFVFKTGDAMGMNMVTIATEKACNFIEGELKKEGIFVKTVAVSGNACVDKKPSGMNLINGRGKSIVAEVFLTEKEVNKYLKTTSQAIAEVNRLKNYIGSAISNSMGFNAHYANIIGAIFLATGQDEAHIVEGSLGITMAEVEDDGLYFSVTLPDVPIGTVGGGTRVETQKECLEMLGCYGDNKALKFAEIVGAAVLAGELSLLGALAAGHLGKAHQELGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q58126</accession>
    <name>Y716_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0716</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0716</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64389">
        <property type="entry name" value="D64389"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58126"/>
    <dbReference type="SMR" id="Q58126"/>
    <dbReference type="STRING" id="243232.MJ_0716"/>
    <dbReference type="EnsemblBacteria" id="AAB98715">
        <property type="protein sequence ID" value="AAB98715"/>
        <property type="gene ID" value="MJ_0716"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0716"/>
    <dbReference type="eggNOG" id="arCOG01688">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2366210_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="SSF111126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107001" description="Uncharacterized protein MJ0716">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <sequence length="95" mass="11014" checksum="2CD4174BE8DA3A5C" modified="1996-11-01" version="1">MEFYNIGDIMALKFTIEELSNQKRDTLGRNIDVTVFRLIRFMDLERYLGRGAHGVIYECGRELGLALNPKTVEDVVKFCEEYKIGKVEIVNKEPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58129</accession>
    <name>Y719_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ0719</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0719</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64389">
        <property type="entry name" value="G64389"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58129"/>
    <dbReference type="SMR" id="Q58129"/>
    <dbReference type="STRING" id="243232.MJ_0719"/>
    <dbReference type="PRIDE" id="Q58129"/>
    <dbReference type="EnsemblBacteria" id="AAB98713">
        <property type="protein sequence ID" value="AAB98713"/>
        <property type="gene ID" value="MJ_0719"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0719"/>
    <dbReference type="eggNOG" id="arCOG00187">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017344_4_1_2"/>
    <dbReference type="InParanoid" id="Q58129"/>
    <dbReference type="OMA" id="ISYKPQY"/>
    <dbReference type="PhylomeDB" id="Q58129"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006415">
        <property type="term" value="P:translational termination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03236">
        <property type="entry name" value="ABC_RNaseL_inhibitor_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034348">
        <property type="entry name" value="RLI_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="PTHR19248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093220" description="Uncharacterized ABC transporter ATP-binding protein MJ0719">
        <location>
            <begin position="1"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="14"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="53"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 1" evidence="1">
        <location>
            <begin position="77"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter 2" evidence="1">
        <location>
            <begin position="348"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="569"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="380"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="600" mass="68019" checksum="07BC8DF6107B354C" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58135</accession>
    <name>Y725_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0725</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0725</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhB family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64390">
        <property type="entry name" value="E64390"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870230.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58135"/>
    <dbReference type="SMR" id="Q58135"/>
    <dbReference type="STRING" id="243232.MJ_0725"/>
    <dbReference type="EnsemblBacteria" id="AAB98721">
        <property type="protein sequence ID" value="AAB98721"/>
        <property type="gene ID" value="MJ_0725"/>
    </dbReference>
    <dbReference type="GeneID" id="1451602"/>
    <dbReference type="KEGG" id="mja:MJ_0725"/>
    <dbReference type="eggNOG" id="arCOG02651">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037958_0_0_2"/>
    <dbReference type="InParanoid" id="Q58135"/>
    <dbReference type="OMA" id="GYEIFNE"/>
    <dbReference type="OrthoDB" id="49654at2157"/>
    <dbReference type="PhylomeDB" id="Q58135"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159227" description="Uncharacterized protein MJ0725">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="261" mass="29017" checksum="10D8066AB1458133" modified="1996-11-01" version="1">MKYLSAKSKLNIDAQDGGFTTTLLSYCLENGILDAVVVVGDKNWKPVAYLATTPTELLKSTKSKYSISPNNKLLEYATENYDKVGLVGLPCHILGGLQFDLTLKVGLFCTKNFYYDTIKSIIKERFGVNIDEVAKMNITKGKFVVETLKKKGFAGTEKVVYEIPIKEIEKLCNLGCRVCTDFSAKYADVSVGSVGSEDGWNTVIVRNKMVEDIINEMAEKGLIEVKETVDIKAIEKLENIKKKNEEINKCSAYFAVCPALF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q58158</accession>
    <name>FPRA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme F420H(2) oxidase</fullName>
            <ecNumber evidence="1">1.5.3.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>FMN protein FprA</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Flavoprotein A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Type A flavoprotein FprA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">fprA</name>
        <name type="ordered locus">MJ0748</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Eur. J. Biochem." volume="254" first="325" last="332">
            <title>A family of flavoproteins in the domains Archaea and Bacteria.</title>
            <authorList>
                <person name="Wasserfallen A."/>
                <person name="Ragettli S."/>
                <person name="Jouanneau Y."/>
                <person name="Leisinger T."/>
            </authorList>
            <dbReference type="PubMed" id="9660187"/>
            <dbReference type="DOI" id="10.1046/j.1432-1327.1998.2540325.x"/>
        </citation>
        <scope>DISCUSSION OF FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of F420H(2) with O(2) (By similarity). May be involved in O(2) detoxification, reducing the intracellular O(2) concentration to a level allowing growth at the expense of methane formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>O2 + 2 reduced coenzyme F420-(gamma-L-Glu)(n) = 2 H(+) + 2 H2O + 2 oxidized coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:39711"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.3.22"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="1">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="1">Binds 2 iron ions per subunit.</text>
    </comment>
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    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
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        <location>
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        <ligand>
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    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q50497"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00088"/>
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    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="416" mass="47380" checksum="DA2C17F0647A2255" modified="1996-11-01" version="1">MKKYESRRSKIADGVYWVGVLDWDIRMYHGYTLKGTTYNAYLVFGDEKVALIDNTYPGTSAQMWGRIKDAFEKEGREFKIDVIVQNHVEKDHSGALPEIHKKFPDAPIYCTEVAVEGLKKHYPSLKDAQFKVVHTGDTVDLGGKTLTFLEAPLLHWPDSMFTFYNEGGILFSNDAFGQHLCFPAHKRFDKDIPEYVLMDANQKFYANLITPLSKLVLKKFEEVIQLGLLEKIKMIAPSHGQIWTDPMKVIKAYQDFATGKAAKDKAVIVYDTMHYSTQKMAHAFAEGLMSEGIDVVMYFLHYDERSEIVKDILDAKAVLFGIPTIYDEPYPSIGDIIYYLRGLKFNRTGFKRLAVTFGSMGGEGGAVAKIAEDLAKCGFEVINQYELYYVPTEDELTNCYNMGKELAKRIKEMKIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q58173</accession>
    <name>Y763_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ0763</fullName>
        </recommendedName>
    </protein>
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        <name type="ordered locus">MJ0763</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanocaldococcus</taxon>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
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            <begin position="1"/>
            <end position="121"/>
        </location>
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</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58183</accession>
    <name>Y773_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ0773</fullName>
        </recommendedName>
    </protein>
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        <name type="ordered locus">MJ0773</name>
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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            </authorList>
            <dbReference type="PubMed" id="8688087"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="SSF46785"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013997" description="Uncharacterized protein MJ0773">
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            <begin position="34"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="175" mass="20247" checksum="63EE09A737EE4F11" modified="1996-11-01" version="1" precursor="true">MERLPYEIVSTIFRKAILHYVLIRGTTYPQSLAENLNISKGLASSFLRLCSALNIMKRERAGHKVLYSFTSKGLAILKRLAPEIFDLSFSSVFEQLPKKKIATKYYPVDKIGFEISWKEDKLGGIVFSFFDSNGEHLGDVFRSNKGYWWCVICQSDTCKHIDYLKRLYKTLKNQD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q58187</accession>
    <name>TFE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Transcription factor E</fullName>
            <shortName>TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName>TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">tfe</name>
        <name type="ordered locus">MJ0777</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="51719" last="51721">
            <title>A fully recombinant system for activator-dependent archaeal transcription.</title>
            <authorList>
                <person name="Ouhammouch M."/>
                <person name="Werner F."/>
                <person name="Weinzierl R.O."/>
                <person name="Geiduschek E.P."/>
            </authorList>
            <dbReference type="PubMed" id="15485836"/>
            <dbReference type="DOI" id="10.1074/jbc.C400446200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2005" name="Mol. Cell. Biol." volume="25" first="8344" last="8355">
            <title>Direct modulation of RNA polymerase core functions by basal transcription factors.</title>
            <authorList>
                <person name="Werner F."/>
                <person name="Weinzierl R.O."/>
            </authorList>
            <dbReference type="PubMed" id="16135821"/>
            <dbReference type="DOI" id="10.1128/MCB.25.18.8344-8355.2005"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH TBP AND RNA POLYMERASE</scope>
    </reference>
    <comment type="function">
        <text evidence="2 3">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer (By similarity). Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with RNA polymerase in the absence of Tbp or DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TFE family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98767" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98767.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58187"/>
    <dbReference type="SMR" id="Q58187"/>
    <dbReference type="STRING" id="243232.MJ_0777"/>
    <dbReference type="EnsemblBacteria" id="AAB98767">
        <property type="protein sequence ID" value="AAB98767"/>
        <property type="gene ID" value="MJ_0777"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0777"/>
    <dbReference type="eggNOG" id="arCOG04270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100097_0_0_2"/>
    <dbReference type="InParanoid" id="Q58187"/>
    <dbReference type="OMA" id="DSGWLTY"/>
    <dbReference type="PhylomeDB" id="Q58187"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001113">
        <property type="term" value="P:transcription open complex formation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="PTHR13097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="TF_Zn_Ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00373">
        <property type="entry name" value="TIGR00373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000107028" description="Transcription factor E">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="HTH TFE/IIEalpha-type">
        <location>
            <begin position="3"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="15485836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16135821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="173" mass="20667" checksum="F78759126582C7B6" modified="2008-02-26" version="3">MLNDPLVQEVLFNIFEGDEKGFEVIDVLLEKGETTEEEIAKELGVKLNVVRKLLYKLYDARLVDYKRWKDEDTNWYSYTWLPTLEKLPYVVKKKINELIKDLEKKLEFEKNNMFFFCPNCNVRFTFEEAMDYGFSCPGCGNMLQEFDNSELIKDLEEQIKFLKEELKNNPFLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58189</accession>
    <name>Y779_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0779</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0779</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64397">
        <property type="entry name" value="C64397"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870284.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58189"/>
    <dbReference type="SMR" id="Q58189"/>
    <dbReference type="STRING" id="243232.MJ_0779"/>
    <dbReference type="EnsemblBacteria" id="AAB98769">
        <property type="protein sequence ID" value="AAB98769"/>
        <property type="gene ID" value="MJ_0779"/>
    </dbReference>
    <dbReference type="GeneID" id="1451656"/>
    <dbReference type="KEGG" id="mja:MJ_0779"/>
    <dbReference type="eggNOG" id="arCOG01811">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_929424_0_0_2"/>
    <dbReference type="InParanoid" id="Q58189"/>
    <dbReference type="OMA" id="NRMIITC"/>
    <dbReference type="OrthoDB" id="60030at2157"/>
    <dbReference type="PhylomeDB" id="Q58189"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107030" description="Uncharacterized protein MJ0779">
        <location>
            <begin position="1"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="299" mass="33291" checksum="2251166CDB6BFAD5" modified="1996-11-01" version="1">MPKYLTTLYKRTIKRNIILFKKLGKDFDEKKFILLLIIIAAIPLLISYYLHLTLKSMIIFVVIYVGAALFIPSILYENKIETLENNIPQALYIMILALESGRSINEALLEVVKSNIKEVSDIFRKVLYLMENQKLSFEESMTIVSNLYDSKVLRMLARIMIENRKYGGDLSDSLKILAKTLEDFKMYKRQLLSVTASGLAIGFIILCGVIPAVAALLGAYLIAVSGMLSGVAPIPPVKPEDISKGFEIVQMGTAIIGALFAIPIFGLKIGRMFLISAVTMTIGVLAYYTILKFAPGIFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58209</accession>
    <name>Y799_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0799</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0799</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To Synechocystis PCC 6803 sll0787 and M.jannaschii MJ0640.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64399">
        <property type="entry name" value="G64399"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58209"/>
    <dbReference type="SMR" id="Q58209"/>
    <dbReference type="STRING" id="243232.MJ_0799"/>
    <dbReference type="EnsemblBacteria" id="AAB98794">
        <property type="protein sequence ID" value="AAB98794"/>
        <property type="gene ID" value="MJ_0799"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0799"/>
    <dbReference type="eggNOG" id="arCOG00642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_948680_0_0_2"/>
    <dbReference type="OMA" id="MEGPYPL"/>
    <dbReference type="PhylomeDB" id="Q58209"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011414">
        <property type="entry name" value="UCP036541_AIR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="PTHR30270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036541">
        <property type="entry name" value="UCP036541_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107050" description="Uncharacterized protein MJ0799">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="296" mass="33054" checksum="7590A47A898F0B22" modified="1996-11-01" version="1">MKMENFEYELKMAIEHILETNYPRKAFWHFDDLIDDLKSGIKAGDDAVVIKNMVINMEGPYPLKLGAKTALIHTACDVVAMGAEPKFALNAIQAKNEDEIKLAVDGLRKQSIGLNIPIIGGNTQTVEELKSCISVAVFGELIDENLIIKDGGAKDGDLLIMLGDPVEGDVGERIYKAKKKFDTYLEILENGIKINACKDASRGGWLGNLLEMLIKAKKGAEIKSLPYPRATRYLGTYIIAVPEEEYEKVVDIALKNKCPVVLFGRILEKPKLIIGTKEYISENKMLELIKKFPYKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58232</accession>
    <name>CSG_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">S-layer protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cell surface glycoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Surface layer protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">sla</name>
        <name type="synonym">slmJ1</name>
        <name type="ordered locus">MJ0822</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Extremophiles" volume="6" first="351" last="358">
            <title>Genes and derived amino acid sequences of S-layer proteins from mesophilic, thermophilic, and extremely thermophilic methanococci.</title>
            <authorList>
                <person name="Akca E."/>
                <person name="Claus H."/>
                <person name="Schultz N."/>
                <person name="Karbach G."/>
                <person name="Schlott B."/>
                <person name="Debaerdemaeker T."/>
                <person name="Declerq J.P."/>
                <person name="Konig H."/>
            </authorList>
            <dbReference type="PubMed" id="12382110"/>
            <dbReference type="DOI" id="10.1007/s00792-001-0264-1"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>PROTEIN SEQUENCE OF 29-38</scope>
        <scope>FUNCTION</scope>
        <scope>SUBCELLULAR LOCATION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="thesis" date="2000" institute="University of Illinois, United States">
            <title>Archaeal gene identification.</title>
            <authorList>
                <person name="Graham D.E."/>
            </authorList>
        </citation>
        <scope>PROTEIN SEQUENCE OF 29-39</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">S-layer protein. The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of the cell.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Cell wall</location>
            <location evidence="2">S-layer</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Mj S-layer protein family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AJ311636">
        <property type="protein sequence ID" value="CAC84089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64402">
        <property type="entry name" value="F64402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870333.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58232"/>
    <dbReference type="SMR" id="Q58232"/>
    <dbReference type="DNASU" id="1451705"/>
    <dbReference type="EnsemblBacteria" id="AAB98821">
        <property type="protein sequence ID" value="AAB98821"/>
        <property type="gene ID" value="MJ_0822"/>
    </dbReference>
    <dbReference type="GeneID" id="1451705"/>
    <dbReference type="KEGG" id="mja:MJ_0822"/>
    <dbReference type="eggNOG" id="arCOG03418">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016296_0_0_2"/>
    <dbReference type="InParanoid" id="Q58232"/>
    <dbReference type="OMA" id="LMFKEGT"/>
    <dbReference type="OrthoDB" id="37561at2157"/>
    <dbReference type="PhylomeDB" id="Q58232"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030115">
        <property type="term" value="C:S-layer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006454">
        <property type="entry name" value="S_layer_MJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022650">
        <property type="entry name" value="S_layer_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05123">
        <property type="entry name" value="S_layer_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01564">
        <property type="entry name" value="S_layer_MJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0134">Cell wall</keyword>
    <keyword id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0325">Glycoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0701">S-layer</keyword>
    <keyword id="KW-0964">Secreted</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2 3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000032620" description="S-layer protein">
        <location>
            <begin position="29"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="138"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <feature type="glycosylation site" description="N-linked (GlcNAc...) asparagine" evidence="1">
        <location>
            <position position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="12382110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source ref="3"/>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="12382110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="558" mass="60548" checksum="CF46C69302BBFB01" modified="1996-11-01" version="1" precursor="true"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q58256</accession>
    <accession>Q977G8</accession>
    <name>MCRA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methyl-coenzyme M reductase I subunit alpha</fullName>
            <shortName>MCR I alpha</shortName>
            <ecNumber evidence="1">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Coenzyme-B sulfoethylthiotransferase alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mcrA</name>
        <name type="ordered locus">MJ0846</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Microbiology" volume="148" first="3521" last="3530">
            <title>The mcrA gene as an alternative to 16S rRNA in the phylogenetic analysis of methanogen populations in landfill.</title>
            <authorList>
                <person name="Luton P.E."/>
                <person name="Wayne J.M."/>
                <person name="Sharp R.J."/>
                <person name="Riley P.W."/>
            </authorList>
            <dbReference type="PubMed" id="12427943"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 340-478</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>coenzyme B + methyl-coenzyme M = coenzyme M-coenzyme B heterodisulfide + methane</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="1">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="2">Is methylated on C5 of Arg-274 by the methyltransferase MJ0841. This post-translational methylation, despite being not essential in vivo, plays a role for the stability and structural integrity of MCR.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyl-coenzyme M reductase alpha subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="AF414040">
        <property type="protein sequence ID" value="AAL29289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870360.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6PEU">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="M/N/P/Q=442-453"/>
    </dbReference>
    <dbReference type="PDBsum" id="6PEU"/>
    <dbReference type="AlphaFoldDB" id="Q58256"/>
    <dbReference type="SMR" id="Q58256"/>
    <dbReference type="STRING" id="243232.MJ_0846"/>
    <dbReference type="PRIDE" id="Q58256"/>
    <dbReference type="EnsemblBacteria" id="AAB98851">
        <property type="protein sequence ID" value="AAB98851"/>
        <property type="gene ID" value="MJ_0846"/>
    </dbReference>
    <dbReference type="GeneID" id="1451734"/>
    <dbReference type="KEGG" id="mja:MJ_0846"/>
    <dbReference type="eggNOG" id="arCOG04857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493170_0_0_2"/>
    <dbReference type="InParanoid" id="Q58256"/>
    <dbReference type="OMA" id="AMLYDQI"/>
    <dbReference type="OrthoDB" id="6589at2157"/>
    <dbReference type="PhylomeDB" id="Q58256"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016212">
        <property type="entry name" value="Me_CoM_Rdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009047">
        <property type="entry name" value="Me_CoM_Rdtase_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02249">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000262">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="SSF48081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="SSF55088"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03256">
        <property type="entry name" value="met_CoM_red_alp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0488">Methylation</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000147452" description="Methyl-coenzyme M reductase I subunit alpha">
        <location>
            <begin position="1"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </ligand>
        <ligandPart>
            <name>Ni</name>
            <dbReference type="ChEBI" id="CHEBI:28112"/>
        </ligandPart>
    </feature>
    <feature type="binding site" description="in chain A" evidence="1">
        <location>
            <position position="228"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <note>ligand shared between two alpha subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in chain A" evidence="1">
        <location>
            <begin position="259"/>
            <end position="260"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <note>ligand shared between two alpha subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in chain B" evidence="1">
        <location>
            <position position="273"/>
        </location>
        <ligand>
            <name>coenzyme B</name>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <note>ligand shared between two alpha subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="335"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>coenzyme M</name>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="5-methylarginine" evidence="2">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 2; AAL29289." evidence="3" ref="2">
        <original>N</original>
        <variation>F</variation>
        <location>
            <position position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P11558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8THH1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="553" mass="61266" checksum="CC3AB9AC4F745A24" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58267</accession>
    <name>Y857_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0857</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0857</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64407">
        <property type="entry name" value="A64407"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58267"/>
    <dbReference type="STRING" id="243232.MJ_0857"/>
    <dbReference type="EnsemblBacteria" id="AAB98863">
        <property type="protein sequence ID" value="AAB98863"/>
        <property type="gene ID" value="MJ_0857"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0857"/>
    <dbReference type="eggNOG" id="arCOG00679">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032903_17_0_2"/>
    <dbReference type="InParanoid" id="Q58267"/>
    <dbReference type="OMA" id="YINGGWS"/>
    <dbReference type="PhylomeDB" id="Q58267"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107082" description="Uncharacterized protein MJ0857">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <sequence length="274" mass="31704" checksum="7C0149CC545F597E" modified="1996-11-01" version="1">MPKTIKLTKTIALKSKPLTKTKIKIIENTIESYKEVLSIALDFGLKNNRKSHRKIREGIYEEIKSKLPKLPTHYIYTASQDASTRIKSFIAMKKRDKAYTSKPKIKNISLWLDDVLTNYRDFKNNIEKLFLIDKEGKKTLHLRLSTPNGRIVIPLKPHKQFFKLLNEGWGIKAGFKLRLNKEDGTITVLIPLEKEITINDSYKTVYALDFNLDNITYGNFENIELIKTDLGKLTEKYSNIMTNIQEKFSFKGIHKQDKPLKRKGFILLKNSVGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58288</accession>
    <name>Y878_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized lipoprotein MJ0878</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0878</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64409">
        <property type="entry name" value="F64409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870392.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58288"/>
    <dbReference type="SMR" id="Q58288"/>
    <dbReference type="STRING" id="243232.MJ_0878"/>
    <dbReference type="EnsemblBacteria" id="AAB98893">
        <property type="protein sequence ID" value="AAB98893"/>
        <property type="gene ID" value="MJ_0878"/>
    </dbReference>
    <dbReference type="GeneID" id="1451767"/>
    <dbReference type="KEGG" id="mja:MJ_0878"/>
    <dbReference type="eggNOG" id="arCOG03417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025776_0_0_2"/>
    <dbReference type="InParanoid" id="Q58288"/>
    <dbReference type="OMA" id="WIKFFGA"/>
    <dbReference type="OrthoDB" id="12936at2157"/>
    <dbReference type="PhylomeDB" id="Q58288"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014005" description="Uncharacterized lipoprotein MJ0878">
        <location>
            <begin position="21"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="119"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-archaeol cysteine" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00344"/>
        </source>
    </evidence>
    <sequence length="414" mass="47112" checksum="BF7F9F2D294E62BF" modified="1996-11-01" version="1" precursor="true">MKKLLAIGILCIMVTAVMSGCVSEKEININNSNKITTNMPVSEKNITKILKYAKNMNLIYYDENGNIVNPYNGDKWKYKVFIDATGQKFLLKNKDDPVPSWAKEKLGDNFKVINVPLTRVIVMSSTEIALMEAINDDGSVIGSVKGIMWGKSYKWYFKDINKSLAEGKIIDVGSSSNPNWDKIIEINPQVIFVYPGYDGDKIIAKCKELGITYVADAEYLENDPLGRCEWVKMFAAFYNKEPEAKRYFEKVEDNCLKVINKTKNCPKVTVAWGYNSQWGCYVPENNSYVAKEIMFYCNGDYIFKDLNGTGSAKINYETFAERAKDADVWVVPSSTAWLSTFKEDNPGYETFKAVKNGRVFCESDDYWQLGLLKTDEVIMDLATILHPEAFKGRKTHFFLKYNIENNTATPFIAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58303</accession>
    <name>FLAB3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Flagellin B3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaB3</name>
        <name type="ordered locus">MJ0893</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal flagellin family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98896" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98896.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64411">
        <property type="entry name" value="E64411"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58303"/>
    <dbReference type="SMR" id="Q58303"/>
    <dbReference type="STRING" id="243232.MJ_0893"/>
    <dbReference type="DNASU" id="1451782"/>
    <dbReference type="EnsemblBacteria" id="AAB98896">
        <property type="protein sequence ID" value="AAB98896"/>
        <property type="gene ID" value="MJ_0893"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0893"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051124_0_1_2"/>
    <dbReference type="InParanoid" id="Q58303"/>
    <dbReference type="OMA" id="TAGKLQH"/>
    <dbReference type="PhylomeDB" id="Q58303"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="PTHR35903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Arch_flagellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="propeptide" id="PRO_0000009377" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000009378" description="Flagellin B3">
        <location>
            <begin position="12"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="216" mass="23053" checksum="A1B36EC8C761DCE0" modified="1997-11-01" version="1" precursor="true">MLLDYIKSRRGAIGIGTLIIFIALVLVAAVAAAVIINTAANLQHKAARVGEESTRQVASGIQVLKITGYAVNTKNITKLAILVSPNVGDEIDLSSTIVTISNGDYKASLVYGGQITYVNTNGTRDIFNESWPNIANPTTEFGVIVLQDADGSMNNTEHPTMNFGDKAIIAINVGDVFGGIMPRERIYGEVIPEFGASGIIEFRAPSTFSEHVVTLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58316</accession>
    <name>Y906_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0906</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0906</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64413">
        <property type="entry name" value="B64413"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58316"/>
    <dbReference type="SMR" id="Q58316"/>
    <dbReference type="STRING" id="243232.MJ_0906"/>
    <dbReference type="EnsemblBacteria" id="AAB98916">
        <property type="protein sequence ID" value="AAB98916"/>
        <property type="gene ID" value="MJ_0906"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0906"/>
    <dbReference type="eggNOG" id="arCOG05058">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052780_0_0_2"/>
    <dbReference type="InParanoid" id="Q58316"/>
    <dbReference type="OMA" id="FRQGLKF"/>
    <dbReference type="PhylomeDB" id="Q58316"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026669">
        <property type="entry name" value="Arsenite_MeTrfase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43675">
        <property type="entry name" value="PTHR43675"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107098" description="Uncharacterized protein MJ0906">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <sequence length="366" mass="42708" checksum="56AABFC1F3FF4727" modified="1996-11-01" version="1">MKFDSAKIENVVGGGSFEIGDDDSKILDEIISEVINDIMPSEIKLKKKIEMIDNTIEYLSYELLVTFIKHGVEFGIFPIIAKYSPKIDDIPLLVKYPNKQFILDYIKTALKLEILKYEDEKIKINEDFELNIKMPKFDKIISDYVMKYNFITHVSRYALISYSHPKIAISFKKDPDIWDMILSSPYYSLCREIASDYLKIDKGDYILDVGCGSRSPKYFIDMIYPEGHYMGVDISKGLLQIAECRIKRLYCDSYELKNIDFTKIIPKEKYDYIICSHTMIYAPSLKQFLNKMMSSIHSGGKIFISEEFILDKNENICKEVFEFYNRLNKRFRGYYSEKDIVDILESLGYDFKIESLGNGILVIEKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q58336</accession>
    <name>Y926_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0926</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0926</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64415">
        <property type="entry name" value="F64415"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58336"/>
    <dbReference type="EnsemblBacteria" id="AAB98938">
        <property type="protein sequence ID" value="AAB98938"/>
        <property type="gene ID" value="MJ_0926"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0926"/>
    <dbReference type="HOGENOM" id="CLU_2476021_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107110" description="Uncharacterized protein MJ0926">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <sequence length="87" mass="10458" checksum="2FD59227A291A76D" modified="1996-11-01" version="1">MELSIENELDKNILSILKDQDLIEDYALKVKIERCNNKYICTCDVIIIQKKLFIKKPVKIEPIKKRLMEILEAKPYNVEYRIDIRLY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58363</accession>
    <name>Y953_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0953</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0953</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64419">
        <property type="entry name" value="A64419"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870467.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58363"/>
    <dbReference type="SMR" id="Q58363"/>
    <dbReference type="STRING" id="243232.MJ_0953"/>
    <dbReference type="EnsemblBacteria" id="AAB98958">
        <property type="protein sequence ID" value="AAB98958"/>
        <property type="gene ID" value="MJ_0953"/>
    </dbReference>
    <dbReference type="GeneID" id="1451851"/>
    <dbReference type="KEGG" id="mja:MJ_0953"/>
    <dbReference type="eggNOG" id="arCOG03099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257387_0_0_2"/>
    <dbReference type="InParanoid" id="Q58363"/>
    <dbReference type="OMA" id="IIPFEPY"/>
    <dbReference type="OrthoDB" id="107043at2157"/>
    <dbReference type="PhylomeDB" id="Q58363"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107119" description="Uncharacterized protein MJ0953">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="100" mass="11192" checksum="1E73563CF1336F91" modified="1996-11-01" version="1">MRLLGVIGYLAVLIKAICESWVDVVKRSINGEIHPQVIEIESIINNPTGLVLLSWSITATPGTLVIDLIPEERKLKVAVISPRSREDIVPFEPYIKKIFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58380</accession>
    <name>CFBA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="1">4.99.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CbiXS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Sirohydrochlorin nickelchelatase</fullName>
            <ecNumber evidence="1">4.99.1.11</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cbiX</name>
        <name evidence="1" type="synonym">cfbA</name>
        <name type="ordered locus">MJ0970</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni-sirohydrochlorin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Co-sirohydrochlorin + 2 H(+) = Co(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:15893"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:60049"/>
            <dbReference type="EC" id="4.99.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 H(+) + Ni-sirohydrochlorin = Ni(2+) + sirohydrochlorin</text>
            <dbReference type="Rhea" id="RHEA:52796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="EC" id="4.99.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CbiX family. CbiXS subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.3" evidence="1"/>
    <dbReference type="EC" id="4.99.1.11" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64421">
        <property type="entry name" value="B64421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870484.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6M25">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M26">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.61 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M27">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M28">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.00 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M29">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M2A">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.23 A"/>
        <property type="chains" value="A/B=1-86, A/B=119-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M2E">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M2F">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M2G">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDB" id="6M2H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.10 A"/>
        <property type="chains" value="A/B=1-143"/>
    </dbReference>
    <dbReference type="PDBsum" id="6M25"/>
    <dbReference type="PDBsum" id="6M26"/>
    <dbReference type="PDBsum" id="6M27"/>
    <dbReference type="PDBsum" id="6M28"/>
    <dbReference type="PDBsum" id="6M29"/>
    <dbReference type="PDBsum" id="6M2A"/>
    <dbReference type="PDBsum" id="6M2E"/>
    <dbReference type="PDBsum" id="6M2F"/>
    <dbReference type="PDBsum" id="6M2G"/>
    <dbReference type="PDBsum" id="6M2H"/>
    <dbReference type="AlphaFoldDB" id="Q58380"/>
    <dbReference type="SMR" id="Q58380"/>
    <dbReference type="STRING" id="243232.MJ_0970"/>
    <dbReference type="PRIDE" id="Q58380"/>
    <dbReference type="EnsemblBacteria" id="AAB98975">
        <property type="protein sequence ID" value="AAB98975"/>
        <property type="gene ID" value="MJ_0970"/>
    </dbReference>
    <dbReference type="GeneID" id="1451868"/>
    <dbReference type="KEGG" id="mja:MJ_0970"/>
    <dbReference type="eggNOG" id="arCOG02246">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_065901_2_1_2"/>
    <dbReference type="InParanoid" id="Q58380"/>
    <dbReference type="OMA" id="HEHEKLE"/>
    <dbReference type="OrthoDB" id="93992at2157"/>
    <dbReference type="PhylomeDB" id="Q58380"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00785">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023652">
        <property type="entry name" value="SiroHydchlorin_Cochelatase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150356" description="Sirohydrochlorin cobaltochelatase">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="2"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="12"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="15"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="49"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="61"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="74"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="79"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="119"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="130"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00785"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="6M2A"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="6M2F"/>
        </source>
    </evidence>
    <sequence length="143" mass="16519" checksum="3B2947A443F25098" modified="1996-11-01" version="1">MEALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAKRIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEIIYREPIGADDRIVDIIIDRAFGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="147" xmlns="http://uniprot.org/uniprot">
    <accession>Q58424</accession>
    <name>SERA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>D-3-phosphoglycerate dehydrogenase</fullName>
            <shortName>PGDH</shortName>
            <ecNumber>1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">serA</name>
        <name type="ordered locus">MJ1018</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64427">
        <property type="entry name" value="A64427"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870531.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58424"/>
    <dbReference type="SMR" id="Q58424"/>
    <dbReference type="STRING" id="243232.MJ_1018"/>
    <dbReference type="PRIDE" id="Q58424"/>
    <dbReference type="EnsemblBacteria" id="AAB99020">
        <property type="protein sequence ID" value="AAB99020"/>
        <property type="gene ID" value="MJ_1018"/>
    </dbReference>
    <dbReference type="GeneID" id="1451915"/>
    <dbReference type="KEGG" id="mja:MJ_1018"/>
    <dbReference type="eggNOG" id="arCOG01754">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019796_8_1_2"/>
    <dbReference type="InParanoid" id="Q58424"/>
    <dbReference type="OMA" id="NIAGMQV"/>
    <dbReference type="OrthoDB" id="24697at2157"/>
    <dbReference type="PhylomeDB" id="Q58424"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="SSF143548"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0718">Serine biosynthesis</keyword>
    <feature type="chain" id="PRO_0000076009" description="D-3-phosphoglycerate dehydrogenase">
        <location>
            <begin position="1"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="3">
        <location>
            <begin position="452"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="231"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="149"/>
            <end position="150"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="229"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="278"/>
            <end position="281"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P0A9T0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="524" mass="56924" checksum="5C561BB68C170793" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58447</accession>
    <name>NUSA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nusA</name>
        <name type="ordered locus">MJ1045</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NusA family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64430">
        <property type="entry name" value="D64430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870558.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58447"/>
    <dbReference type="SMR" id="Q58447"/>
    <dbReference type="STRING" id="243232.MJ_1045"/>
    <dbReference type="EnsemblBacteria" id="AAB99049">
        <property type="protein sequence ID" value="AAB99049"/>
        <property type="gene ID" value="MJ_1045"/>
    </dbReference>
    <dbReference type="GeneID" id="1451942"/>
    <dbReference type="KEGG" id="mja:MJ_1045"/>
    <dbReference type="eggNOG" id="arCOG01760">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131906_1_0_2"/>
    <dbReference type="InParanoid" id="Q58447"/>
    <dbReference type="OMA" id="TDQIKYI"/>
    <dbReference type="OrthoDB" id="99842at2157"/>
    <dbReference type="PhylomeDB" id="Q58447"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription, termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025249">
        <property type="entry name" value="KH_dom_NusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="NusA_bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="PTHR22648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13184">
        <property type="entry name" value="KH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="SSF54814"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0806">Transcription termination</keyword>
    <feature type="chain" id="PRO_0000181983" description="Probable transcription termination protein NusA">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="KH" evidence="1">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="149"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="183" mass="21135" checksum="2845502CAD695783" modified="1997-11-01" version="1">MAKVRLTTEEIMKIGFFEKIANVPILDCVLNDERVAFIVKEGDVGAAIGKGGENVKTAEEKFGKKVDIIEYSDDWRKFIRNIFAPIQLDDVWVKRVGKDVVAFIKINPKVRRAVFGEKGKNLERALKILKRHTKITKIKVIVENQKFKRKRAKRPVVKDQQQEQTETKQETDVQQDVKETVKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58467</accession>
    <name>Y1068_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized membrane protein MJ1068</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1068</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polysaccharide synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64433">
        <property type="entry name" value="B64433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870580.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58467"/>
    <dbReference type="SMR" id="Q58467"/>
    <dbReference type="STRING" id="243232.MJ_1068"/>
    <dbReference type="EnsemblBacteria" id="AAB99070">
        <property type="protein sequence ID" value="AAB99070"/>
        <property type="gene ID" value="MJ_1068"/>
    </dbReference>
    <dbReference type="GeneID" id="1451964"/>
    <dbReference type="KEGG" id="mja:MJ_1068"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022017_5_0_2"/>
    <dbReference type="InParanoid" id="Q58467"/>
    <dbReference type="OMA" id="GLMPQFI"/>
    <dbReference type="OrthoDB" id="41033at2157"/>
    <dbReference type="PhylomeDB" id="Q58467"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029303">
        <property type="entry name" value="Polysacc_synt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14667">
        <property type="entry name" value="Polysacc_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000166460" description="Uncharacterized membrane protein MJ1068">
        <location>
            <begin position="1"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="355"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="387"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="408"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="444"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="467"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="507" mass="57664" checksum="A345A38772278235" modified="1996-11-01" version="1">MSYKEKAVKGVSWHLLSYFLAAPIAYLVRVLYANEIPKLDVGLFYAVLDFFSMLVVFRAFGLDQALIRYIPKYLAENRLDMLKSSIVFVGILQTILAFIVAFLVVIFAPYIAEFYINNQGQFTGRLDLVINILIIMAMGYYFLDSIVAFFSNILTGFQLQNYASSTRVVRILSVFIFSLIFIYLFNVHNAYVPSVSYLLMAVVMIIIYGYIVVKKIFPKFAKEKVIFSRKLIRNLFSYGMYVMIGYAGSLILGYLDGICLTYFTGLNAVADYRNVAMPTVNILSYFAFSVGAVLFPMSSELWEKGYKKALSYGVEKVFLYSLIIVTPLAILMAYFPTVIINILFNPKYLSAAPAIQILSFGAMFLTFNSIGFNILNGIGRPNISTKILYIGASFNLIFNILLIPKFGIIGAAITTVFGYFIMWIFQIWFLNKLLEHQFLNKKWILVILVGIFSLIPVMFIKDLIDNVILQLFVCGVVYFGIYILGIFGLKIINIYEVKDIISKIIKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58486</accession>
    <name>Y1086_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1086</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1086</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64435">
        <property type="entry name" value="E64435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870598.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58486"/>
    <dbReference type="STRING" id="243232.MJ_1086"/>
    <dbReference type="PRIDE" id="Q58486"/>
    <dbReference type="DNASU" id="1451982"/>
    <dbReference type="EnsemblBacteria" id="AAB99087">
        <property type="protein sequence ID" value="AAB99087"/>
        <property type="gene ID" value="MJ_1086"/>
    </dbReference>
    <dbReference type="GeneID" id="1451982"/>
    <dbReference type="KEGG" id="mja:MJ_1086"/>
    <dbReference type="eggNOG" id="arCOG01831">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072733_0_0_2"/>
    <dbReference type="InParanoid" id="Q58486"/>
    <dbReference type="OMA" id="FTHTYAG"/>
    <dbReference type="OrthoDB" id="40897at2157"/>
    <dbReference type="PhylomeDB" id="Q58486"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107164" description="Uncharacterized protein MJ1086">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <sequence length="340" mass="40555" checksum="CC82FB3F988358C1" modified="1996-11-01" version="1">MKVRIRDFIETTEGLYFAVNTYAHPKNKFFAFLRYVPYEFVDFKIEDNNIREINGRKYIKMAESKIAYKFLEEKFSKYLYYDETINVLMHAIPKEDVKRILRPKERLNEIINEENNLNELEEKCRKLALILEDYGVPIKSMGVSGSLLLKLNNKNSDIDFVIYGKDMHKKAREALKQAFEDNKLKPLSDDFWKIAYKKRIKDKTLTYEEFVFYEKRKYNRGIVDNTMFDLLFTREWDEITEKYGDKRYKNLGFAKIEGRVLNDDFAFDNPAVYKIECYNDEDIKEVVSFTHTYAGQCFNGEEIVARGKLEEVIDKSGERYKRIVVGTTREAFNEYIKIKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58522</accession>
    <name>VAPB3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative antitoxin VapB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">vapB3</name>
        <name type="ordered locus">MJ1122</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possibly the antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is VapC3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0330 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64440">
        <property type="entry name" value="A64440"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58522"/>
    <dbReference type="SMR" id="Q58522"/>
    <dbReference type="STRING" id="243232.MJ_1122"/>
    <dbReference type="EnsemblBacteria" id="AAB99124">
        <property type="protein sequence ID" value="AAB99124"/>
        <property type="gene ID" value="MJ_1122"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1122"/>
    <dbReference type="eggNOG" id="arCOG02681">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170073_3_0_2"/>
    <dbReference type="InParanoid" id="Q58522"/>
    <dbReference type="OMA" id="IMIRDEV"/>
    <dbReference type="PhylomeDB" id="Q58522"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00794">
        <property type="entry name" value="UPF0330"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003847">
        <property type="entry name" value="Put_antitoxin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02697">
        <property type="entry name" value="VAPB_antitox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000157108" description="Putative antitoxin VapB3">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00794"/>
        </source>
    </evidence>
    <sequence length="77" mass="9135" checksum="461C4E194C235556" modified="1996-11-01" version="1">MINMATITIDDDVYKELLKLKGRKSVSEFIKELLEERKRKNLDVFMIAFGSRSEEDVEKLKKELKEAEKWMQSLIQV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-08-03" version="145" xmlns="http://uniprot.org/uniprot">
    <accession>Q58524</accession>
    <name>HELS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ATP-dependent DNA helicase Hel308</fullName>
            <ecNumber evidence="2">3.6.4.12</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Endonuclease PI-MjaHel</fullName>
                <ecNumber>3.1.-.-</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>Mja Hel intein</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Mja Pep3 intein</fullName>
            </alternativeName>
        </component>
    </protein>
    <gene>
        <name evidence="2" type="primary">hel308</name>
        <name type="ordered locus">MJ1124</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">DNA-dependent ATPase and 3'-5' DNA helicase that may be involved in repair of stalled replication forks.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the helicase family. Hel308 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="2"/>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64440">
        <property type="entry name" value="C64440"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870635.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58524"/>
    <dbReference type="SMR" id="Q58524"/>
    <dbReference type="STRING" id="243232.MJ_1124"/>
    <dbReference type="PRIDE" id="Q58524"/>
    <dbReference type="EnsemblBacteria" id="AAB99126">
        <property type="protein sequence ID" value="AAB99126"/>
        <property type="gene ID" value="MJ_1124"/>
    </dbReference>
    <dbReference type="GeneID" id="1452020"/>
    <dbReference type="KEGG" id="mja:MJ_1124"/>
    <dbReference type="eggNOG" id="arCOG00553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_279743_0_0_2"/>
    <dbReference type="InParanoid" id="Q58524"/>
    <dbReference type="OMA" id="CLNANTE"/>
    <dbReference type="OrthoDB" id="9752at2157"/>
    <dbReference type="PhylomeDB" id="Q58524"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006314">
        <property type="term" value="P:intron homing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00442">
        <property type="entry name" value="Helicase_Hel308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022965">
        <property type="entry name" value="Helicase_Hel308"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0404">Intron homing</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000013299" description="ATP-dependent DNA helicase Hel308, 1st part" evidence="1">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013300" description="Endonuclease PI-MjaHel" evidence="1">
        <location>
            <begin position="338"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013301" description="ATP-dependent DNA helicase Hel308, 2nd part" evidence="1">
        <location>
            <begin position="839"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="2">
        <location>
            <begin position="26"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease">
        <location>
            <begin position="451"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="1195" mass="138674" checksum="87C9CF1A87EFB52D" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="156" xmlns="http://uniprot.org/uniprot">
    <accession>Q58530</accession>
    <name>KAE1B_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="1">tRNA N6-adenosine threonylcarbamoyltransferase</fullName>
                <ecNumber evidence="1">2.3.1.234</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>N6-L-threonylcarbamoyladenine synthase</fullName>
                <shortName>t(6)A synthase</shortName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="1">tRNA threonylcarbamoyladenosine biosynthesis protein Kae1</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="1">Serine/threonine-protein kinase Bud32</fullName>
                <ecNumber evidence="1">2.7.11.1</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name type="ordered locus">MJ1130</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="EMBO J." volume="27" first="2340" last="2351">
            <title>Structure of the archaeal Kae1/Bud32 fusion protein MJ1130: a model for the eukaryotic EKC/KEOPS subcomplex.</title>
            <authorList>
                <person name="Hecker A."/>
                <person name="Lopreiato R."/>
                <person name="Graille M."/>
                <person name="Collinet B."/>
                <person name="Forterre P."/>
                <person name="Libri D."/>
                <person name="van Tilbeurgh H."/>
            </authorList>
            <dbReference type="PubMed" id="19172740"/>
            <dbReference type="DOI" id="10.1038/emboj.2008.157"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) IN COMPLEX WITH AN ATP ANALOG</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="Mol. Cell" volume="32" first="259" last="275">
            <title>Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine.</title>
            <authorList>
                <person name="Mao D.Y."/>
                <person name="Neculai D."/>
                <person name="Downey M."/>
                <person name="Orlicky S."/>
                <person name="Haffani Y.Z."/>
                <person name="Ceccarelli D.F."/>
                <person name="Ho J.S."/>
                <person name="Szilard R.K."/>
                <person name="Zhang W."/>
                <person name="Ho C.S."/>
                <person name="Wan L."/>
                <person name="Fares C."/>
                <person name="Rumpel S."/>
                <person name="Kurinov I."/>
                <person name="Arrowsmith C.H."/>
                <person name="Durocher D."/>
                <person name="Sicheri F."/>
            </authorList>
            <dbReference type="PubMed" id="18951093"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2008.10.002"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.67 ANGSTROMS) IN COMPLEXES WITH MAGNESIUM AND CGI121</scope>
        <scope>FUNCTION</scope>
        <scope>SUBUNIT</scope>
        <scope>ACTIVITY REGULATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction (By similarity). The Bud32 domain probably displays kinase activity that regulates Kae1 function. In vitro, exhibits low ATPase activity, but does not bind DNA and does not have endonuclease activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:37059"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.3.1.234"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Activity provided by the Kae1 region seems to be regulated via phosphorylation by the protein kinase Bud32, which is itself activated by Cgi121.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3">Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the KAE1 / TsaD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.234" evidence="1"/>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870641.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2VWB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.05 A"/>
        <property type="chains" value="A/B=1-535"/>
    </dbReference>
    <dbReference type="PDB" id="3EN9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.67 A"/>
        <property type="chains" value="A/B=1-535"/>
    </dbReference>
    <dbReference type="PDB" id="3ENH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.60 A"/>
        <property type="chains" value="A/B=1-535"/>
    </dbReference>
    <dbReference type="PDB" id="5JMV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.39 A"/>
        <property type="chains" value="A/B/C=1-335"/>
    </dbReference>
    <dbReference type="PDBsum" id="2VWB"/>
    <dbReference type="PDBsum" id="3EN9"/>
    <dbReference type="PDBsum" id="3ENH"/>
    <dbReference type="PDBsum" id="5JMV"/>
    <dbReference type="AlphaFoldDB" id="Q58530"/>
    <dbReference type="SMR" id="Q58530"/>
    <dbReference type="STRING" id="243232.MJ_1130"/>
    <dbReference type="EnsemblBacteria" id="AAB99132">
        <property type="protein sequence ID" value="AAB99132"/>
        <property type="gene ID" value="MJ_1130"/>
    </dbReference>
    <dbReference type="GeneID" id="1452026"/>
    <dbReference type="KEGG" id="mja:MJ_1130"/>
    <dbReference type="eggNOG" id="arCOG01183">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01185">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023208_2_2_2"/>
    <dbReference type="InParanoid" id="Q58530"/>
    <dbReference type="OMA" id="GETMDTG"/>
    <dbReference type="OrthoDB" id="9881at2157"/>
    <dbReference type="PhylomeDB" id="Q58530"/>
    <dbReference type="EvolutionaryTrace" id="Q58530"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061711">
        <property type="term" value="F:N(6)-L-threonylcarbamoyladenine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006468">
        <property type="term" value="P:protein phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01446">
        <property type="entry name" value="Kae1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01447">
        <property type="entry name" value="Kae1_Bud32_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000905">
        <property type="entry name" value="Gcp-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017861">
        <property type="entry name" value="KAE1/TsaD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022449">
        <property type="entry name" value="Kae1_arc"/>
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    <sequence length="535" mass="60570" checksum="B18EEFACE8E5A99F" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58535</accession>
    <name>MOAC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="1">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">moaC</name>
        <name type="ordered locus">MJ1135</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaC family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB99137.1"/>
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    <dbReference type="GeneID" id="1452031"/>
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    <dbReference type="HOGENOM" id="CLU_074693_1_2_2"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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        </location>
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            <begin position="72"/>
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        <ligand>
            <name>substrate</name>
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        <ligand>
            <name>substrate</name>
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        </source>
    </evidence>
    <sequence length="152" mass="17043" checksum="D91AC8ED1D2852AF" modified="1996-11-01" version="1">MLTHVDDKGVKMVDISKKEDVERICVAEGYIKLKPETIKLIKEQKIKKGNVLTTAQIAGILAVKKTYELIPMCHPLPITSVNVDFEVFEDKIKAICSVKTTYKTGIEMEALTGVSIALLTIWDMVKSAEKDEDGQYKTAEIFGIRVVEKIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58541</accession>
    <name>Y1141_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1141</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1141</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64442">
        <property type="entry name" value="D64442"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58541"/>
    <dbReference type="STRING" id="243232.MJ_1141"/>
    <dbReference type="EnsemblBacteria" id="AAB99141">
        <property type="protein sequence ID" value="AAB99141"/>
        <property type="gene ID" value="MJ_1141"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1141"/>
    <dbReference type="eggNOG" id="arCOG02078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067052_1_0_2"/>
    <dbReference type="InParanoid" id="Q58541"/>
    <dbReference type="OMA" id="CPAKLTP"/>
    <dbReference type="PhylomeDB" id="Q58541"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002829">
        <property type="entry name" value="DUF116"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43801">
        <property type="entry name" value="PTHR43801"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01976">
        <property type="entry name" value="DUF116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006594">
        <property type="entry name" value="UCP006594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107186" description="Uncharacterized protein MJ1141">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="214" mass="24430" checksum="AC0C4D0301F95228" modified="1996-11-01" version="1">MISILGLDGFLQLVGMITIAIFALAFISFILILIISYILLKKNKLIFPSLALFLMDNLYSILLKIFLLIGTEDTFYRVGIEFYNKYYEDRFKKAKKRVLILPHCLRDTKCPAKLTPKGVECIFCNRCRVGEIIKVAEEKGYKVYIVPGSTFLKRILKEEKPEAVFGVACNRDLFYGMNMLSRKGIPSQGQPLLRDGCINTLVDVDELITRLKSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58543</accession>
    <name>MSMJL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Large-conductance mechanosensitive channel MscMJLR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1143</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="EMBO J." volume="20" first="1888" last="1896">
            <title>Structural and functional differences between two homologous mechanosensitive channels of Methanococcus jannaschii.</title>
            <authorList>
                <person name="Kloda A."/>
                <person name="Martinac B."/>
            </authorList>
            <dbReference type="PubMed" id="11296222"/>
            <dbReference type="DOI" id="10.1093/emboj/20.8.1888"/>
        </citation>
        <scope>FUNCTION AS A MECHANOSENSITIVE CHANNEL</scope>
        <scope>SUBCELLULAR LOCATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Large-conductance mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. Selective for cations. Rectifies with voltage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64442">
        <property type="entry name" value="F64442"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870654.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58543"/>
    <dbReference type="SMR" id="Q58543"/>
    <dbReference type="STRING" id="243232.MJ_1143"/>
    <dbReference type="TCDB" id="1.A.23.4.1">
        <property type="family name" value="the small conductance mechanosensitive ion channel (mscs) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99143">
        <property type="protein sequence ID" value="AAB99143"/>
        <property type="gene ID" value="MJ_1143"/>
    </dbReference>
    <dbReference type="GeneID" id="1452039"/>
    <dbReference type="KEGG" id="mja:MJ_1143"/>
    <dbReference type="eggNOG" id="arCOG01568">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037945_0_1_2"/>
    <dbReference type="InParanoid" id="Q58543"/>
    <dbReference type="OMA" id="QNYKRLQ"/>
    <dbReference type="OrthoDB" id="58511at2157"/>
    <dbReference type="PhylomeDB" id="Q58543"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006686">
        <property type="entry name" value="MscS_channel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="SSF50182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="SSF82689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="SSF82861"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01246">
        <property type="entry name" value="UPF0003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000110254" description="Large-conductance mechanosensitive channel MscMJLR">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11296222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="361" mass="40683" checksum="A6339C3C8AC4D68E" modified="1996-11-01" version="1">MTITQMISEILMHNTVYNYILSLISIILFIVIGKYANALIERLADKLHKKSGIELDELLIRALSLPVAIAIILSGFYFGVNFLYLLPSLKTAVNEGILTAFILCVVVFFDRFLNELVERYLALTISKKTKKDVDDQIVVLTKKLVRLVVWVVGLLLILSNLGYDIKTLLAGLGIGGLAVALASQNLVSNLIAGLIILTDKPFKIGNWITFSGGSGIVEDIGIRSTKIRATDNSIIVVPNSKLIDEIIQNVPSKNKWKVSTTIGVTYNTPVEKIRKAEEIIKNILLEHPNVEDEPITVYFKEFGDWSLNIQVVYYIKNSRYNGYQKYISTINEVNLKIKEEFDRKGIEFAFPTYTLYLKRDD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q58548</accession>
    <name>TFS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor S</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Transcription elongation factor IIS/RNA polymerase subunit homolog</fullName>
            <shortName evidence="1">TFIIS/RPSU homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tfs</name>
        <name type="ordered locus">MJ1148</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Induces RNA cleavage activity in the RNA polymerase. In its presence, the cleavage activity of the RNA polymerase truncates the RNA back to position +15 in a stepwise manner by releasing mainly dinucleotides from the 3'-end of the nascent RNA. The truncated RNAs are able to continue elongation. Involved in transcriptional proofreading and fidelity. Misincorporation of nucleotides during elongation of transcription leads to arrested elongation complexes which are rescued by TFS-promoted removal of a dinucleotide from the 3'-end. TFS is able to induce a cleavage resynthesis cycle in stalled elongation complexes (resulting from the next missing nucleotide or a reduced incorporation rate of a wrong nucleotide) preventing misincorporation and enabling proofreading in a post-incorporation manner. Pausing of elongation complexes is the main determinant of TFS-induced RNA cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">More similar by sequence similarity to the eukaryotic RNA polymerase subunits.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64443">
        <property type="entry name" value="C64443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870659.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58548"/>
    <dbReference type="SMR" id="Q58548"/>
    <dbReference type="STRING" id="243232.MJ_1148"/>
    <dbReference type="PRIDE" id="Q58548"/>
    <dbReference type="EnsemblBacteria" id="AAB99148">
        <property type="protein sequence ID" value="AAB99148"/>
        <property type="gene ID" value="MJ_1148"/>
    </dbReference>
    <dbReference type="GeneID" id="1452044"/>
    <dbReference type="KEGG" id="mja:MJ_1148"/>
    <dbReference type="eggNOG" id="arCOG00579">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093932_3_2_2"/>
    <dbReference type="InParanoid" id="Q58548"/>
    <dbReference type="OMA" id="MVKFCPK"/>
    <dbReference type="OrthoDB" id="121393at2157"/>
    <dbReference type="PhylomeDB" id="Q58548"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="DNA-dir_RNA_pol_M/15kDasu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="PTHR11239"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="TFIIS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000121481" description="Transcription factor S">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="2">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TFIIS-type" evidence="3">
        <location>
            <begin position="65"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q9P9I8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="108" mass="12750" checksum="33AE963C576AD1BC" modified="1996-11-01" version="1">MVKFCPKCNNLMLPKDGKLKCAVCGYEEETTAEGSKEYEYKEHLENKKEKITVIESEGLETLPTTRIECPKCGHNEAYWWLQQTRCADEPETRFYKCKKCGHTWREYD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58552</accession>
    <name>Y1152_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1152</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1152</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64443">
        <property type="entry name" value="G64443"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870663.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58552"/>
    <dbReference type="SMR" id="Q58552"/>
    <dbReference type="STRING" id="243232.MJ_1152"/>
    <dbReference type="EnsemblBacteria" id="AAB99158">
        <property type="protein sequence ID" value="AAB99158"/>
        <property type="gene ID" value="MJ_1152"/>
    </dbReference>
    <dbReference type="GeneID" id="1452048"/>
    <dbReference type="KEGG" id="mja:MJ_1152"/>
    <dbReference type="eggNOG" id="arCOG09567">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2766168_0_0_2"/>
    <dbReference type="OMA" id="GHEKYVE"/>
    <dbReference type="OrthoDB" id="143685at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107190" description="Uncharacterized protein MJ1152">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <sequence length="73" mass="8760" checksum="C7116019D1CE55E7" modified="1996-11-01" version="1">MVIMKKHVKRIVKKYLGSKKAESEEEDILPDWIVKVKNEIENITYEEYVKDVDKFFNEVKKKGIEKVLFDDIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58560</accession>
    <name>GATA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName>Glu-ADT subunit A</shortName>
            <ecNumber>6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">gatA</name>
        <name type="ordered locus">MJ1160</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64444">
        <property type="entry name" value="G64444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870673.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58560"/>
    <dbReference type="SMR" id="Q58560"/>
    <dbReference type="STRING" id="243232.MJ_1160"/>
    <dbReference type="EnsemblBacteria" id="AAB99163">
        <property type="protein sequence ID" value="AAB99163"/>
        <property type="gene ID" value="MJ_1160"/>
    </dbReference>
    <dbReference type="GeneID" id="1452058"/>
    <dbReference type="KEGG" id="mja:MJ_1160"/>
    <dbReference type="eggNOG" id="arCOG01717">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009600_0_3_2"/>
    <dbReference type="InParanoid" id="Q58560"/>
    <dbReference type="OMA" id="EVSCPHF"/>
    <dbReference type="OrthoDB" id="12800at2157"/>
    <dbReference type="PhylomeDB" id="Q58560"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="PTHR11895"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="SSF75304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000105233" description="Glutamyl-tRNA(Gln) amidotransferase subunit A">
        <location>
            <begin position="1"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="1">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="1">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="434" mass="48008" checksum="0A95BF0C62161F5F" modified="1997-11-01" version="1">MIVERVEEYLDRIEKINKDINALIEVKPEKVLEEAKKLEKDEKAKKKPLYGKIIVVKANINVEGYTISCASKTLENYIAPYDATVIEKIKENGGLIIGIANMDEFACGSSGETSYFGPTKNPRAKDRIPGGSSSGSAAAVSADLCDMALGSDTGGSIRNPASHCGVVGFKPSYGVVSRYGLCDLAMSFDQIGPLTKTAEDALLLTNIIKGKDLRDTTTVETKPFEKKDIKGFKVGVVKEFMDVADEKIRDKVEKAIEVFKDLGCEIVELSYKYVDLALPTYYLINYVEFFSSTRRYDGRRYGYKIEEVCGEEVLRRIMIGSMISQKEYSGKYYKNALKARNLMRNEMIKIMKDVDIIVGATVPKLPHKLGEKLTPMEMYSYDVLTVPANICGLCAGVVPCGDINGIPVGLQIQGKPFEDEKVLSAMIAFEKAME</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58561</accession>
    <name>Y1161_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1161</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1161</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64444">
        <property type="entry name" value="H64444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870674.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58561"/>
    <dbReference type="STRING" id="243232.MJ_1161"/>
    <dbReference type="EnsemblBacteria" id="AAB99175">
        <property type="protein sequence ID" value="AAB99175"/>
        <property type="gene ID" value="MJ_1161"/>
    </dbReference>
    <dbReference type="GeneID" id="1452059"/>
    <dbReference type="KEGG" id="mja:MJ_1161"/>
    <dbReference type="eggNOG" id="arCOG09677">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718240_0_0_2"/>
    <dbReference type="OMA" id="DYQVING"/>
    <dbReference type="OrthoDB" id="129576at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107197" description="Uncharacterized protein MJ1161">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="167" mass="18977" checksum="7A3CA6409CCC6640" modified="1996-11-01" version="1">MRDNIRSINKLLIYLVLILSAMFLCQNVFAEDYNAIEITVKNISSGEILYQKIFPKDEAFSDYQVINGFTIDIHYNPNYPGMVYKVHQSNNHFIFNVSGELNWGSYQQSGDIACIVDVIHYDFPPPSNTTSNTTTTSSSSTKAPIPLSVYLAITAFFTYIIYRKSKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58568</accession>
    <name>FWDD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Protein FwdD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fwdD</name>
        <name type="ordered locus">MJ1168</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64445">
        <property type="entry name" value="G64445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870681.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58568"/>
    <dbReference type="SMR" id="Q58568"/>
    <dbReference type="STRING" id="243232.MJ_1168"/>
    <dbReference type="EnsemblBacteria" id="AAB99170">
        <property type="protein sequence ID" value="AAB99170"/>
        <property type="gene ID" value="MJ_1168"/>
    </dbReference>
    <dbReference type="GeneID" id="1452066"/>
    <dbReference type="KEGG" id="mja:MJ_1168"/>
    <dbReference type="eggNOG" id="arCOG02674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123704_1_0_2"/>
    <dbReference type="InParanoid" id="Q58568"/>
    <dbReference type="OMA" id="GMIYIPM"/>
    <dbReference type="OrthoDB" id="91239at2157"/>
    <dbReference type="PhylomeDB" id="Q58568"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02789">
        <property type="entry name" value="MopB_CT_FmdC-FwdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041717">
        <property type="entry name" value="FmdC/FwdD_MopB-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012040">
        <property type="entry name" value="Formylmethanofuran_DH_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015873">
        <property type="entry name" value="FwdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000087393" description="Protein FwdD">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <sequence length="133" mass="15207" checksum="39D14E35D18E935F" modified="1996-11-01" version="1">MKFFLNTGRTIWQGEAMEAGKNLDLYVKAAGVVYINEEDMEKLGVKEGDKVKVKSEYGEVVVYVKKATERMPEGMIYIPMGPWANCVVKPDTHSTGMPTFKGYPGFYVEVEKTDEEFLDMRSLMRKKYIEAVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58607</accession>
    <name>Y1210_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1210</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1210</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64451">
        <property type="entry name" value="A64451"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58607"/>
    <dbReference type="SMR" id="Q58607"/>
    <dbReference type="STRING" id="243232.MJ_1210"/>
    <dbReference type="DNASU" id="1452106"/>
    <dbReference type="EnsemblBacteria" id="AAB99213">
        <property type="protein sequence ID" value="AAB99213"/>
        <property type="gene ID" value="MJ_1210"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1210"/>
    <dbReference type="eggNOG" id="arCOG00348">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075000_1_0_2"/>
    <dbReference type="InParanoid" id="Q58607"/>
    <dbReference type="OMA" id="ICLMGET"/>
    <dbReference type="PhylomeDB" id="Q58607"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="CHP00062"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00162">
        <property type="entry name" value="TIGR00162"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107214" description="Uncharacterized protein MJ1210">
        <location>
            <begin position="1"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="258" mass="28708" checksum="62E71561D4950050" modified="1996-11-01" version="1">MMFMVKIITRKVKDIEPLENALLIEGLPGIGHVGRLAAEHLVHEFKGEKFLELFCYDFPPQVLVKDDGTIEYMCAEFYAIREPKPMIVVLGNTQALSPIGQYHLAEEIVKIGIEYGANFVYTLGGFGVGKLCEEVKVYGATTSKELAKKLKEHDILFRTDGGGIVGAAGLMLMFADLNGIPGICLMGETPGYLIDPNAAKAVLEKFCKLENIEINMEELEKRAKGMEQFIEKIKKFEEEMLKAAQAKPPSEEDLRYIG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58613</accession>
    <name>Y1216_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative RNase MJ1216</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative toxin MJ1216</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1216</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable toxic component of a putative type VII toxin-antitoxin (TA) system, probably an RNase. Probably neutralized by antitoxin MJ1215 or MJ1217. Neutralization may be due to AMPylation by antitoxin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer, probably forms a complex with antitoxin MJ1215 or MJ1217.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Modified by antitoxin MJ1215 or MJ1217; probably at least 2 successive AMPylation events occur on Tyr-83.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64451">
        <property type="entry name" value="G64451"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870728.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58613"/>
    <dbReference type="SMR" id="Q58613"/>
    <dbReference type="STRING" id="243232.MJ_1216"/>
    <dbReference type="EnsemblBacteria" id="AAB99217">
        <property type="protein sequence ID" value="AAB99217"/>
        <property type="gene ID" value="MJ_1216"/>
    </dbReference>
    <dbReference type="GeneID" id="1452112"/>
    <dbReference type="KEGG" id="mja:MJ_1216"/>
    <dbReference type="eggNOG" id="arCOG05024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142825_3_3_2"/>
    <dbReference type="InParanoid" id="Q58613"/>
    <dbReference type="OMA" id="WRAMAGM"/>
    <dbReference type="OrthoDB" id="112390at2157"/>
    <dbReference type="PhylomeDB" id="Q58613"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000158263" description="Putative RNase MJ1216">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RX(4)HXY motif" evidence="1">
        <location>
            <begin position="76"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-di-AMP-tyrosine" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJR1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="121" mass="14498" checksum="F95805B623D85298" modified="1997-11-01" version="1">MPKRDIKAFLYDILEYMDDIINFTKNMEYEEFINNKAIKYAVVRCLEVIGEAVKKIPKDIREKYPHIPFKELAGMRDKLIHQYFGVDYLTVWETAKYEIPEIKKEFEKIIKDIEGKDENSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q58672</accession>
    <name>ILVD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="1">DAD</shortName>
            <ecNumber evidence="1">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ilvD</name>
        <name type="ordered locus">MJ1276</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:27695"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99282" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99282.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64459">
        <property type="entry name" value="C64459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496775.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58672"/>
    <dbReference type="SMR" id="Q58672"/>
    <dbReference type="STRING" id="243232.MJ_1276"/>
    <dbReference type="EnsemblBacteria" id="AAB99282">
        <property type="protein sequence ID" value="AAB99282"/>
        <property type="gene ID" value="MJ_1276"/>
    </dbReference>
    <dbReference type="GeneID" id="1452174"/>
    <dbReference type="KEGG" id="mja:MJ_1276"/>
    <dbReference type="eggNOG" id="arCOG04045">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014271_4_2_2"/>
    <dbReference type="InParanoid" id="Q58672"/>
    <dbReference type="OMA" id="TQGRNMA"/>
    <dbReference type="OrthoDB" id="7176at2157"/>
    <dbReference type="PhylomeDB" id="Q58672"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="SSF143975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000103541" description="Dihydroxy-acid dehydratase">
        <location>
            <begin position="1"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="467"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="553" mass="59198" checksum="9FF5127928919E60" modified="2002-07-11" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58697</accession>
    <name>Y1301_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ1301</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1301</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64462">
        <property type="entry name" value="D64462"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58697"/>
    <dbReference type="STRING" id="243232.MJ_1301"/>
    <dbReference type="DNASU" id="1452203"/>
    <dbReference type="EnsemblBacteria" id="AAB99326">
        <property type="protein sequence ID" value="AAB99326"/>
        <property type="gene ID" value="MJ_1301"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1301"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q58697"/>
    <dbReference type="OMA" id="EMEYILH"/>
    <dbReference type="PhylomeDB" id="Q58697"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184675" description="Uncharacterized ATP-binding protein MJ1301">
        <location>
            <begin position="1"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="400" mass="46904" checksum="EE9460CAD791D049" modified="1997-11-01" version="1">MGILVIFMKFFNREKEIHKILSIIEGEPNLIYFIYGSINSGKTALINEIINNRLDKNKYIVFYFDLREIFISKYDDFIEVLFEEYEGDKSPIEVIKAIINDLPSLYGIPIPKNTLNEIFKKKTTKNVFRYITNVLMDIKREGKQPIIIIDELQKIGDMKINGFLIYELFNYFVSLTKHKHLCHVFCLSSDSLFIERVYNEAMLEDRVDYILVDDHRGGYAPSIGILPQIESGVAFGNPALEYSNRGFASMRGEYILVDDFDKETALKFMDFLAKENNMSLTNEDKELIYNYVGGKPVLIIKVIDKLRYENLNDILDFMLKDATQKLKYFLEDVKEEDEELYKKVVDALKLFKEDYEIEDIKIPKKIREFLIKRNILFLNPIEGILKPQSFLVWNAIKKVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58724</accession>
    <name>MTM4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II methyltransferase M.MjaIV</fullName>
            <shortName evidence="1">M.MjaIV</shortName>
            <ecNumber>2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">mjaIVMP</name>
        <name type="ordered locus">MJ1328</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">A methylase that recognizes the double-stranded sequence 5'-GTNNAC-3', methylates A-5 on both strands, and protects the DNA from cleavage by the MjaIV endonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.1.1.72"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64465">
        <property type="entry name" value="G64465"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58724"/>
    <dbReference type="SMR" id="Q58724"/>
    <dbReference type="STRING" id="243232.MJ_1328"/>
    <dbReference type="REBASE" id="3895">
        <property type="entry name" value="M.MjaIV"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99337">
        <property type="protein sequence ID" value="AAB99337"/>
        <property type="gene ID" value="MJ_1328"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1328"/>
    <dbReference type="eggNOG" id="arCOG03521">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_932594_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58724"/>
    <dbReference type="PRO" id="PR:Q58724"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000088020" description="Type II methyltransferase M.MjaIV">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <sequence length="298" mass="35328" checksum="9BE52F35B001822B" modified="1996-11-01" version="1">MQKNQTIFLNTWKFLITLHQKPWSTFFTKIPDFPHVLLKDIAKVGVGLVSGFDEAFLLNEDDISKLNEDEKQLIKNFVKAKNCKRFVVEGFVQYILIEDNLKDEEIFKTKYPNIYKKLLKFKDRMENRYLPKNKKWFNWQALRNYKFLIKNLNKKRIYVPTLDRKPYNRFSLGDDELLPSGDVIFIQPYNDDDIYFLLGYLNSSFFRNYYLANGGRRGGRVAFTQKLLENAKIPTFSDEVKEKIKNIVKDIIYNLKNGKDIENLERQIDYIIVSAINNNQFKGYQTTLKNLLKPKLKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58736</accession>
    <name>Y1340_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1340</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1340</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0310 and MJ0714.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64467">
        <property type="entry name" value="C64467"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870858.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58736"/>
    <dbReference type="SMR" id="Q58736"/>
    <dbReference type="STRING" id="243232.MJ_1340"/>
    <dbReference type="EnsemblBacteria" id="AAB99350">
        <property type="protein sequence ID" value="AAB99350"/>
        <property type="gene ID" value="MJ_1340"/>
    </dbReference>
    <dbReference type="GeneID" id="1452243"/>
    <dbReference type="KEGG" id="mja:MJ_1340"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118613_4_0_2"/>
    <dbReference type="InParanoid" id="Q58736"/>
    <dbReference type="OMA" id="NVGRIRY"/>
    <dbReference type="OrthoDB" id="103616at2157"/>
    <dbReference type="PhylomeDB" id="Q58736"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="PTHR13323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107284" description="Uncharacterized protein MJ1340">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="114" mass="11886" checksum="55030A1155D57BEE" modified="1996-11-01" version="1">MIDRVLLELNKTEGIKGSMVVGKDGLVIASQLPGSVDAELVGAMASAAFGAAERTAAEIGMGTLEQTMIEGEHGKTLMVDAGEGILVVLTDAKVNLGLIRITMKRAAEKIKAMF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58737</accession>
    <name>Y1341_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1341</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1341</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64467">
        <property type="entry name" value="D64467"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870859.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58737"/>
    <dbReference type="SMR" id="Q58737"/>
    <dbReference type="EnsemblBacteria" id="AAB99351">
        <property type="protein sequence ID" value="AAB99351"/>
        <property type="gene ID" value="MJ_1341"/>
    </dbReference>
    <dbReference type="GeneID" id="1452244"/>
    <dbReference type="KEGG" id="mja:MJ_1341"/>
    <dbReference type="eggNOG" id="arCOG00116">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893144_0_0_2"/>
    <dbReference type="OMA" id="SPKIINC"/>
    <dbReference type="OrthoDB" id="85237at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107285" description="Uncharacterized protein MJ1341">
        <location>
            <begin position="1"/>
            <end position="312"/>
        </location>
    </feature>
    <sequence length="312" mass="35425" checksum="8D01DAB275CDB249" modified="1996-11-01" version="1">MILKHRRPNIYGLMNKEGNKEEVEMIINELLNRDYKITFLPSGSSAVFLSMWIAKIYSNEISIPDMGGWQGFLKFPKLLNLKNNMIETNLGIIDLEKLDESLKENSSLILTSLAGYLAPQPLKEIKKLCEEREVLFIEDISGKIGGDCGYGDIVVCSTGTPKILNCEYGGFLGISKEIEEKLGNALNDIKILSKTYKTINYFGLLKEELLNAKKTYKKYVEASKIIKDEIENAYFREFEGISVFIECDNPKNISKKINSLIKLDNRKSITTICPNYDRILKNGIVFETKKIDISELNREVINEIIIALSSIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58766</accession>
    <name>Y1371_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized methyltransferase MJ1371</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1371</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtxX family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64471">
        <property type="entry name" value="B64471"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58766"/>
    <dbReference type="SMR" id="Q58766"/>
    <dbReference type="STRING" id="243232.MJ_1371"/>
    <dbReference type="EnsemblBacteria" id="AAB99379">
        <property type="protein sequence ID" value="AAB99379"/>
        <property type="gene ID" value="MJ_1371"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1371"/>
    <dbReference type="eggNOG" id="arCOG00854">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086562_0_0_2"/>
    <dbReference type="InParanoid" id="Q58766"/>
    <dbReference type="OMA" id="GVDEGWT"/>
    <dbReference type="PhylomeDB" id="Q58766"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016764">
        <property type="entry name" value="MeTrfase_MtxX_xsu"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019709">
        <property type="entry name" value="Methyltransf_MtxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03270">
        <property type="entry name" value="methan_mark_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000135933" description="Uncharacterized methyltransferase MJ1371">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="244" mass="27353" checksum="1354A2D00731E2C3" modified="1996-11-01" version="1">MIIMYAIGIGDNKEEVLKAYEKLKEEGIEVELIDNPKLLVDKLLDGEIDGAVRGSLSSSKVILYLRERIGKFYRASILKNPFTNGIFLLSPVGIDDISEDKNERIKDKIRIIEFASNFLKNYNIKAKVAVLSGGRLGDLGRNKVVDETIYEAEEIVEHFKGNVDIIHNGILIEEYLKDGYNIIIAVDGITGNLIFRCLGLICKIPGYGAVILSDKNVNFIDTSRNANWERYYNAIKFLIGGDFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58785</accession>
    <name>RPOI_METJA</name>
    <protein>
        <recommendedName>
            <fullName>DNA-directed RNA polymerase subunit I</fullName>
            <ecNumber>2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpoI</name>
        <name type="ordered locus">MJ1390</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.7.6"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64473">
        <property type="entry name" value="E64473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870907.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58785"/>
    <dbReference type="SMR" id="Q58785"/>
    <dbReference type="STRING" id="243232.MJ_1390"/>
    <dbReference type="EnsemblBacteria" id="AAB99398">
        <property type="protein sequence ID" value="AAB99398"/>
        <property type="gene ID" value="MJ_1390"/>
    </dbReference>
    <dbReference type="GeneID" id="1452293"/>
    <dbReference type="KEGG" id="mja:MJ_1390"/>
    <dbReference type="eggNOG" id="arCOG09634">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2271089_0_0_2"/>
    <dbReference type="OMA" id="KIIMVRR"/>
    <dbReference type="OrthoDB" id="139203at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000074040" description="DNA-directed RNA polymerase subunit I">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <sequence length="109" mass="12677" checksum="E9A807C289430E4F" modified="1996-11-01" version="1">MKGMDEYEKIINDLNTINSKAKFIGIKIIMVRRIIDMHKDNDKLIKKVLEGIKNTDLYDLVLNACPELKGERIKDVYFKKNDYFNVIKKTMSSENTVLKNVLINDESPP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58886</accession>
    <name>Y1491_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1491</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1491</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64486">
        <property type="entry name" value="B64486"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58886"/>
    <dbReference type="STRING" id="243232.MJ_1491"/>
    <dbReference type="EnsemblBacteria" id="AAB99510">
        <property type="protein sequence ID" value="AAB99510"/>
        <property type="gene ID" value="MJ_1491"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1491"/>
    <dbReference type="eggNOG" id="arCOG04907">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151706_0_0_2"/>
    <dbReference type="OMA" id="DYENCGL"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107374" description="Uncharacterized protein MJ1491">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="127" mass="13006" checksum="6D3FB594A7961C96" modified="1996-11-01" version="1">MKYMVSFDQERMLKPAVIGGIINGILGAICCLCYVIGGAVAAHLYVNAGGICDYENCGLVGAISGVIGGVIASILSFLFMSAYLSALGLKAAMFTGASFIIGFITAIIFGAILGAVGGVIYVVIKNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58903</accession>
    <name>Y1508_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ1508</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1508</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64488">
        <property type="entry name" value="C64488"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58903"/>
    <dbReference type="SMR" id="Q58903"/>
    <dbReference type="STRING" id="243232.MJ_1508"/>
    <dbReference type="PRIDE" id="Q58903"/>
    <dbReference type="EnsemblBacteria" id="AAB99520">
        <property type="protein sequence ID" value="AAB99520"/>
        <property type="gene ID" value="MJ_1508"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1508"/>
    <dbReference type="eggNOG" id="arCOG00922">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q58903"/>
    <dbReference type="OMA" id="ATMLKPN"/>
    <dbReference type="PhylomeDB" id="Q58903"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03255">
        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093226" description="Uncharacterized ABC transporter ATP-binding protein MJ1508">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="2"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="38"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="224" mass="25264" checksum="CF0B72742D24921E" modified="1996-11-01" version="1">MIEAKNVWKIYGKGEAKTIALKNINLKIEEGEFVMIMGPSGCGKSTLLNILALLDTPTKGEVYYKGRRTSSMSENERAIFRRKISGFIFQQFHLIKTLTALENVELPMMLDERDKSYRRKRAKKLLEMVGLGDRLNHYPHQLSGGQQQRVAIARALANNPKIIFADEPTGNLDSKSGMAVMSILKGLNEKGITIIMVTHEQELTKYASKIIKLRDGEIVEIINK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q58913</accession>
    <name>Y1518_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1518</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1518</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64489">
        <property type="entry name" value="E64489"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58913"/>
    <dbReference type="STRING" id="243232.MJ_1518"/>
    <dbReference type="EnsemblBacteria" id="AAB99549">
        <property type="protein sequence ID" value="AAB99549"/>
        <property type="gene ID" value="MJ_1518"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1518"/>
    <dbReference type="HOGENOM" id="CLU_738912_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58913"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107390" description="Uncharacterized protein MJ1518">
        <location>
            <begin position="1"/>
            <end position="374"/>
        </location>
    </feature>
    <sequence length="374" mass="44817" checksum="6D678115913A8BFA" modified="1996-11-01" version="1">MLSIIFGSKPREDEIDSVDRYMTKLFLRIIFIPDYTNKIHDLSENVSKNIKNLIMELKSGNIFEINKILDDLEYNIYTIDDESLTIKERVYNAVEIIFKVVSNEILRTESIDDKELEIIKKLHELVVLTDKVLRITYNSLNRSLEDINSYRRIIANNNLPDDIIRRLNIINAYIEKIKSDILSMRENEQRVLIICLSKFYNLYLSKDKKEREKLADEIKNLIEYLYFDWYYDFIDNIGEYEFKFIKNIAAMCLLYDYTKELEKDGSKEMFNHVIFKILRNGGFKVKEYDIIETLLNIRENLPKSSKIDVRYFDKELIKIIRGIVRESKKDVFYNNMKRLKEFVEELREMEKKLSEANNITLHDLRKLGFNDMFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58947</accession>
    <name>Y1552_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1552</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1552</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64493">
        <property type="entry name" value="G64493"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58947"/>
    <dbReference type="SMR" id="Q58947"/>
    <dbReference type="STRING" id="243232.MJ_1552"/>
    <dbReference type="DNASU" id="1452460"/>
    <dbReference type="EnsemblBacteria" id="AAB99572">
        <property type="protein sequence ID" value="AAB99572"/>
        <property type="gene ID" value="MJ_1552"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1552"/>
    <dbReference type="eggNOG" id="arCOG01285">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_119412_2_2_2"/>
    <dbReference type="InParanoid" id="Q58947"/>
    <dbReference type="OMA" id="IYYGTKF"/>
    <dbReference type="PhylomeDB" id="Q58947"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="DUF35_OB-fold_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="DUF35_Znr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="DUF35_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_aCoA_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107408" description="Uncharacterized protein MJ1552">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <sequence length="141" mass="16390" checksum="11690E4E7922D751" modified="1998-06-01" version="2">MGIKYLLRDKMVVRSWRHIKERYCLIGVRCKNCGTVYFPSREICPKCRRKTEFEEIKLSGKGKVYTYSVVHVAPKDFEKQAPYVIAIIELEEGARITGQIVDCKPEDVYIGMQVEAVFRRIKEDGDDGVITYGYKFKPIEN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58975</accession>
    <name>Y1580_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1580</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1580</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.thermoautotrophicum MTH137.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64497">
        <property type="entry name" value="C64497"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58975"/>
    <dbReference type="STRING" id="243232.MJ_1580"/>
    <dbReference type="EnsemblBacteria" id="AAB99600">
        <property type="protein sequence ID" value="AAB99600"/>
        <property type="gene ID" value="MJ_1580"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1580"/>
    <dbReference type="eggNOG" id="arCOG05092">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_134280_4_0_2"/>
    <dbReference type="InParanoid" id="Q58975"/>
    <dbReference type="OMA" id="IAINERY"/>
    <dbReference type="PhylomeDB" id="Q58975"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019277">
        <property type="entry name" value="DUF2304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10066">
        <property type="entry name" value="DUF2304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107425" description="Uncharacterized protein MJ1580">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="124" mass="14644" checksum="B9F5B537435BCB62" modified="1996-11-01" version="1">MLIKILEEKLMELIQIVGVIFALFALSRVVLQLKRRSISFNEGLFWIFVWGFVVIFLVFPEFFGYVAEVLGVGRGVDALIYISIVVLFYLIYRLYAKINNLERQITHIVREIAIRDRYEPKKRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q59016</accession>
    <name>Y1621_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1621</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1621</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64502">
        <property type="entry name" value="D64502"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59016"/>
    <dbReference type="SMR" id="Q59016"/>
    <dbReference type="EnsemblBacteria" id="AAB99647">
        <property type="protein sequence ID" value="AAB99647"/>
        <property type="gene ID" value="MJ_1621"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1621"/>
    <dbReference type="eggNOG" id="arCOG02209">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2985688_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107443" description="Uncharacterized protein MJ1621">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="57" mass="6422" checksum="076F438483AED617" modified="1996-11-01" version="1">MPTVNILSYFAFSVEAVLFPMSSELWEKGYREALGYGVEKICLYSFVLVLPIAILMA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q59020</accession>
    <name>RQCH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Archaeal Rqc2 homolog aRqcH</fullName>
            <shortName evidence="1 2">aRqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">rqcH</name>
        <name type="ordered locus">MJ1625</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2019" name="Cell" volume="0" first="0" last="0">
            <title>Alanine Tails Signal Proteolysis in Bacterial Ribosome-Associated Quality Control.</title>
            <authorList>
                <person name="Lytvynenko I."/>
                <person name="Paternoga H."/>
                <person name="Thrun A."/>
                <person name="Balke A."/>
                <person name="Mueller T.A."/>
                <person name="Chiang C.H."/>
                <person name="Nagler K."/>
                <person name="Tsaprailis G."/>
                <person name="Anders S."/>
                <person name="Bischofs I."/>
                <person name="Maupin-Furlow J.A."/>
                <person name="Spahn C.M.T."/>
                <person name="Joazeiro C.A.P."/>
            </authorList>
            <dbReference type="PubMed" id="31155236"/>
            <dbReference type="DOI" id="10.1016/j.cell.2019.05.002"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Probably part of the ribosome quality control system (RQC). May mediate the addition of alanine residues (Ala tailing) to incompletely synthesized nascent chains from stalled ribosomes, leading to their degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NEMF family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64502">
        <property type="entry name" value="H64502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871150.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59020"/>
    <dbReference type="SMR" id="Q59020"/>
    <dbReference type="STRING" id="243232.MJ_1625"/>
    <dbReference type="PRIDE" id="Q59020"/>
    <dbReference type="EnsemblBacteria" id="AAB99643">
        <property type="protein sequence ID" value="AAB99643"/>
        <property type="gene ID" value="MJ_1625"/>
    </dbReference>
    <dbReference type="GeneID" id="1452535"/>
    <dbReference type="KEGG" id="mja:MJ_1625"/>
    <dbReference type="eggNOG" id="arCOG01695">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003612_2_1_2"/>
    <dbReference type="InParanoid" id="Q59020"/>
    <dbReference type="OMA" id="MVYGKRN"/>
    <dbReference type="OrthoDB" id="69351at2157"/>
    <dbReference type="PhylomeDB" id="Q59020"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_A">
        <property type="entry name" value="RqcH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_S13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043681">
        <property type="entry name" value="RqcH_archaeal"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="SSF46946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0820">tRNA-binding</keyword>
    <feature type="chain" id="PRO_0000107445" description="Archaeal Rqc2 homolog aRqcH">
        <location>
            <begin position="1"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="291"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="410"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="31155236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3">
        <source>
            <dbReference type="PubMed" id="31155236"/>
        </source>
    </evidence>
    <sequence length="671" mass="78616" checksum="49BB5EE873CF6A86" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-06-06" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q59040</accession>
    <name>PYREL_METJA</name>
    <protein>
        <recommendedName>
            <fullName>PyrE-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1646</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64505">
        <property type="entry name" value="D64505"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59040"/>
    <dbReference type="SMR" id="Q59040"/>
    <dbReference type="STRING" id="243232.MJ_1646"/>
    <dbReference type="EnsemblBacteria" id="AAB99667">
        <property type="protein sequence ID" value="AAB99667"/>
        <property type="gene ID" value="MJ_1646"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1646"/>
    <dbReference type="eggNOG" id="arCOG00028">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111001_0_0_2"/>
    <dbReference type="InParanoid" id="Q59040"/>
    <dbReference type="OMA" id="DIHVDWS"/>
    <dbReference type="PhylomeDB" id="Q59040"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006222">
        <property type="term" value="P:UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01214">
        <property type="entry name" value="PyrE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022854">
        <property type="entry name" value="PyrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF41">
        <property type="entry name" value="PTHR19278:SF41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="SSF88659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000110810" description="PyrE-like protein">
        <location>
            <begin position="1"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="211" mass="23306" checksum="61693F216CB2CEF1" modified="1996-11-01" version="1">MREIMNKELLKKVIELKSNGLTIGEIAEELNVSMETARYLVLNAEKLLKEEEKAIKLENVDIFIDWKNIGSSANRLKYISSIIVDILKSRNIEFDTVVGVSTSGVPIATLVASELGKELTIYIPKKHISEEGKKITGSISQNFSAVNYKRAVIIDDVVTSGSTLKECIKQLKEVCSPKLVVVLIDKSGLDEIEGVPLIPLIRIGAVNVEQK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q59049</accession>
    <name>HPRT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hypoxanthine/guanine phosphoribosyltransferase</fullName>
            <shortName evidence="1">HGPRTase</shortName>
            <ecNumber evidence="1">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hpt</name>
        <name type="ordered locus">MJ1655</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + GMP = 5-phospho-alpha-D-ribose 1-diphosphate + guanine</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. Archaeal HPRT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64506">
        <property type="entry name" value="E64506"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871179.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59049"/>
    <dbReference type="SMR" id="Q59049"/>
    <dbReference type="STRING" id="243232.MJ_1655"/>
    <dbReference type="EnsemblBacteria" id="AAB99676">
        <property type="protein sequence ID" value="AAB99676"/>
        <property type="gene ID" value="MJ_1655"/>
    </dbReference>
    <dbReference type="GeneID" id="1452564"/>
    <dbReference type="KEGG" id="mja:MJ_1655"/>
    <dbReference type="eggNOG" id="arCOG00030">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126376_0_0_2"/>
    <dbReference type="InParanoid" id="Q59049"/>
    <dbReference type="OMA" id="FIHPISD"/>
    <dbReference type="OrthoDB" id="88302at2157"/>
    <dbReference type="PhylomeDB" id="Q59049"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043103">
        <property type="term" value="P:hypoxanthine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01467">
        <property type="entry name" value="Hypx_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026597">
        <property type="entry name" value="HGPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00103">
        <property type="entry name" value="PUR_PYR_PR_TRANSFER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0660">Purine salvage</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000149496" description="Hypoxanthine/guanine phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01467"/>
        </source>
    </evidence>
    <sequence length="183" mass="20218" checksum="A20172DC74A941FB" modified="1997-11-01" version="1">MLLEETLKSCPIVKRGEYHYFIHPISDGVPVVEPKLLREVATRIIKIGDFEGATKLVTAEAMGIPLVTTLSLYTDIPYVIMRKREYKLPGEIPVFQSTGYSKGQLYLNGIEKGDKVVIIDDVISTGGTMIAIIDALKRAGAEIKDIICVIERGEGKKIVEEKTGYKIKTLVKIDVVDGKVVIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-10" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q59052</accession>
    <name>Y1658_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1658</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1658</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64506">
        <property type="entry name" value="H64506"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871182.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59052"/>
    <dbReference type="STRING" id="243232.MJ_1658"/>
    <dbReference type="EnsemblBacteria" id="AAB99685">
        <property type="protein sequence ID" value="AAB99685"/>
        <property type="gene ID" value="MJ_1658"/>
    </dbReference>
    <dbReference type="GeneID" id="1452567"/>
    <dbReference type="KEGG" id="mja:MJ_1658"/>
    <dbReference type="eggNOG" id="arCOG06554">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_144581_0_0_2"/>
    <dbReference type="OMA" id="IFISEMD"/>
    <dbReference type="OrthoDB" id="124231at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020380">
        <property type="entry name" value="Uncharacterised_MJ1658"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17393">
        <property type="entry name" value="DUF5402"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107461" description="Uncharacterized protein MJ1658">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <sequence length="119" mass="14042" checksum="607B4444AB5FF512" modified="1996-11-01" version="1">MKDKILKDREEIEKFFIKLLGREIFISEMDIFASRCGCVGIMITVRGLFVDDVEIFKEKILNKLEELAKSYDINADWIFVRVLPGSDDIINIGVREFCNICREEYRFKKPRPDLISLKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-09-19" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q59059</accession>
    <name>Y1665_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0210 protein MJ1665</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1665</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0210 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64507">
        <property type="entry name" value="G64507"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871189.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59059"/>
    <dbReference type="SMR" id="Q59059"/>
    <dbReference type="STRING" id="243232.MJ_1665"/>
    <dbReference type="EnsemblBacteria" id="AAB99687">
        <property type="protein sequence ID" value="AAB99687"/>
        <property type="gene ID" value="MJ_1665"/>
    </dbReference>
    <dbReference type="GeneID" id="1452574"/>
    <dbReference type="KEGG" id="mja:MJ_1665"/>
    <dbReference type="eggNOG" id="arCOG04321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048704_0_0_2"/>
    <dbReference type="InParanoid" id="Q59059"/>
    <dbReference type="OMA" id="EMAIGMV"/>
    <dbReference type="OrthoDB" id="26259at2157"/>
    <dbReference type="PhylomeDB" id="Q59059"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd08025">
        <property type="entry name" value="RNR_PFL_like_DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01221">
        <property type="entry name" value="UPF0210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="PTHR37560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000070564" description="UPF0210 protein MJ1665">
        <location>
            <begin position="1"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01221"/>
        </source>
    </evidence>
    <sequence length="458" mass="48599" checksum="2B7DECC92BB06501" modified="1996-11-01" version="1">MFRPEEIIETIKMIKMENLDLRTVTLGLSLRDCVSKDLDELKENIYNKITSSAENLVETAERISEKYGIPIVNKRIAVTPISLVIGGAIKDLDKEEQIKACVEVGEVLDKAAKKVRVDFLGGYSALVHKDATKEDRALIDSIPFMMEKTERVCSSVNVASTKTGINMDAVKRMGEIIKETAFRTEKAIGCAKLVVFANAPEDNPFMAGAFHGVGEGDKVINVGVSGPGVVRAVIEKLPDADFGTLANEIKKVAFKITRVGELIGREVSKELGVKFGVVDLSLAPTPARGDSIANILEAMGLEKCGTHGSTAALALLNDAVKKGGAMATSYVGGLSGAFIPVSEDSGMVEAVEAGALTLEKLEAMTCVCSVGIDMVAIPGDTPASTISAIIADEMAIGVINNKTTAVRIIPVPGKKAGEYVDYGGLLGKAPIMEVNKYSSEKFIKRGGRIPAPLQALTN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q60179</accession>
    <name>TRPB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Tryptophan synthase beta chain</fullName>
            <ecNumber>4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trpB</name>
        <name type="ordered locus">MJ1037</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrpB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64429">
        <property type="entry name" value="D64429"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60179"/>
    <dbReference type="SMR" id="Q60179"/>
    <dbReference type="STRING" id="243232.MJ_1037"/>
    <dbReference type="PRIDE" id="Q60179"/>
    <dbReference type="EnsemblBacteria" id="AAB99040">
        <property type="protein sequence ID" value="AAB99040"/>
        <property type="gene ID" value="MJ_1037"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1037"/>
    <dbReference type="eggNOG" id="arCOG01433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_3_1_2"/>
    <dbReference type="InParanoid" id="Q60179"/>
    <dbReference type="OMA" id="HGMKSYF"/>
    <dbReference type="PhylomeDB" id="Q60179"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="PLP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="Trypto_synt_PLP_dependent"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="SSF53686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000099038" description="Tryptophan synthase beta chain">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="404" mass="44364" checksum="A75D876F95EA8529" modified="1997-11-01" version="1">MSILKKYKDMYPDENGKFGIYGGKFVPETLMPAIAELEEAFKRFWINNEGNFREEFYALLRDYVGRPTPLYYAERLSEELGCKVYLKREDLAHLGAHKINNALGQALLAKKMGKKRVIAETGAGQHGVATAAACAKLGLECIIYMGAKDVERQKLNVFRMELMGAKVIPVFGGSQTLKDAVNEALRDWTTNVRTTYYLLGSALGPHPYPMMVREFQRVIGKELKEQILEKEGRLPDVIVACVGGGSNAIGAFYEFLDDDVELYAVEAGGKGIETGMHGASLCAGEVGVLHGAKIYVKEDEFGQIEESYSISAGLDYPGVGPELSFLKDEGRIKAVCVTDDEALEAFQLLCRLEGILPALESSHALAYAVKLADKLDKDDIMVINLSGRGDKDVQTVAKALGREI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q60275</accession>
    <name>Y3513_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized MCM-type protein MJECL13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL13</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64511">
        <property type="entry name" value="E64511"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890060.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60275"/>
    <dbReference type="SMR" id="Q60275"/>
    <dbReference type="STRING" id="243232.MJ_ECL13"/>
    <dbReference type="EnsemblBacteria" id="AAC37086">
        <property type="protein sequence ID" value="AAC37086"/>
        <property type="gene ID" value="MJ_ECL13"/>
    </dbReference>
    <dbReference type="GeneID" id="1450799"/>
    <dbReference type="KEGG" id="mja:MJ_ECL13"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_2"/>
    <dbReference type="InParanoid" id="Q60275"/>
    <dbReference type="OrthoDB" id="3067at2157"/>
    <dbReference type="PhylomeDB" id="Q60275"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032508">
        <property type="term" value="P:DNA duplex unwinding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000194132" description="Uncharacterized MCM-type protein MJECL13">
        <location>
            <begin position="1"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="MCM">
        <location>
            <begin position="271"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="315"/>
            <end position="322"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="602" mass="69323" checksum="07C1EABB3D305AD3" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q60305</accession>
    <name>Y3406_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS06</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS06</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJECL27.</text>
    </comment>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64516">
        <property type="entry name" value="F64516"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890099.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60305"/>
    <dbReference type="SMR" id="Q60305"/>
    <dbReference type="STRING" id="243232.MJ_ECS06"/>
    <dbReference type="PRIDE" id="Q60305"/>
    <dbReference type="EnsemblBacteria" id="AAC37064">
        <property type="protein sequence ID" value="AAC37064"/>
        <property type="gene ID" value="MJ_ECS06"/>
    </dbReference>
    <dbReference type="GeneID" id="1450833"/>
    <dbReference type="KEGG" id="mja:MJ_ECS06"/>
    <dbReference type="eggNOG" id="arCOG12102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1801728_0_0_2"/>
    <dbReference type="OrthoDB" id="136478at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <keyword id="KW-0863">Zinc-finger</keyword>
    <feature type="chain" id="PRO_0000107487" description="Uncharacterized protein MJECS06">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C2H2-type">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="140" mass="16592" checksum="DDD50F3AEC9A690E" modified="1997-11-01" version="1">MNIDDYIEKLEIQKEGFFYKCPYCNYTNADVKAIKKHIKSKHYDIIAKEVENLNKQNKPQRKPMKKQPKKKDDDYKDYMLLFAHKKKCKIYLDNGMIVEGTVKAKDRFNIMVLDAKVDDKEVERIIIQKGHIVALIPLEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q60309</accession>
    <name>Y3410_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS10</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64517">
        <property type="entry name" value="B64517"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890103.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60309"/>
    <dbReference type="EnsemblBacteria" id="AAC37068">
        <property type="protein sequence ID" value="AAC37068"/>
        <property type="gene ID" value="MJ_ECS10"/>
    </dbReference>
    <dbReference type="GeneID" id="1450837"/>
    <dbReference type="KEGG" id="mja:MJ_ECS10"/>
    <dbReference type="HOGENOM" id="CLU_971851_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107491" description="Uncharacterized protein MJECS10">
        <location>
            <begin position="1"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="286" mass="33271" checksum="E4A26112108033A9" modified="1997-11-01" version="1">MNFKKVSNMPDEIICNFYVNISYLEISATYDSKNKRYSLSSKTGNFNVGVKGFEKLLSIFEKIGHVMKINEKTYKIIIQNKILNLKHLYSKKNKTHTLEILDEYNNLMKLSGKKLIDVALQLFKTFKINENIENSLNVKKTENDIQIINNNQNLKIEENINDSKTKENDNKCYREIIDRVLKSRSEQLFIENLENIPPKSVIYSITGAFSFIFGLGVLCETLESGKIRWKAIILGFIILILILAIVNYLMMKEKNVDEHEYNIPTKLMEDVYTTDCENFPCGYFPK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q60354</accession>
    <name>Y046_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative methyltransferase MJ0046</fullName>
            <ecNumber>2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0046</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64305">
        <property type="entry name" value="F64305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869537.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60354"/>
    <dbReference type="SMR" id="Q60354"/>
    <dbReference type="STRING" id="243232.MJ_0046"/>
    <dbReference type="EnsemblBacteria" id="AAB98026">
        <property type="protein sequence ID" value="AAB98026"/>
        <property type="gene ID" value="MJ_0046"/>
    </dbReference>
    <dbReference type="GeneID" id="1450884"/>
    <dbReference type="KEGG" id="mja:MJ_0046"/>
    <dbReference type="eggNOG" id="arCOG00111">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071987_0_0_2"/>
    <dbReference type="InParanoid" id="Q60354"/>
    <dbReference type="OMA" id="PTPINRY"/>
    <dbReference type="OrthoDB" id="52481at2157"/>
    <dbReference type="PhylomeDB" id="Q60354"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052907">
        <property type="term" value="F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="PTHR13393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000218022" description="Putative methyltransferase MJ0046">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="261" mass="29931" checksum="E46C39551D2C34CF" modified="1997-11-01" version="1">MLGLKIEDAIKYNEKLKKYVYKKGDKLRINFKDKEALIEYNKTVLKVLFDLDIEFHKNGLIPTPINRYLFIKSTFETLKELGIEKPTVLEIGTGHSAIISLLIKKFYNAEVYATEVDEEFIDFAKRNIEKNKLDIKIINSKGRAIEGIEELKDKKFDLIISYPPFYSKNSVASGRKFGGALAKNVELIGGGKFGEEFSFKIIEEGINFLNKKGVISLMMPKKPEKRRELIIKKMKEVGLDVEVDEIKTGNRLRYIIKGIKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q60378</accession>
    <name>Y072_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0072</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0072</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64308">
        <property type="entry name" value="H64308"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60378"/>
    <dbReference type="SMR" id="Q60378"/>
    <dbReference type="STRING" id="243232.MJ_0072"/>
    <dbReference type="EnsemblBacteria" id="AAB98054">
        <property type="protein sequence ID" value="AAB98054"/>
        <property type="gene ID" value="MJ_0072"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0072"/>
    <dbReference type="eggNOG" id="arCOG05114">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3210744_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106680" description="Uncharacterized protein MJ0072">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <sequence length="44" mass="4889" checksum="C742DB9EBBC1F451" modified="1997-11-01" version="1">MIILLDLNGTIATDGKIKEGVKERLTILKERAEIYILSADTSEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>P54010</accession>
    <name>RL32_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L32e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl32e</name>
        <name type="ordered locus">MJ0472</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64358">
        <property type="entry name" value="H64358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869973.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54010"/>
    <dbReference type="SMR" id="P54010"/>
    <dbReference type="STRING" id="243232.MJ_0472"/>
    <dbReference type="EnsemblBacteria" id="AAB98461">
        <property type="protein sequence ID" value="AAB98461"/>
        <property type="gene ID" value="MJ_0472"/>
    </dbReference>
    <dbReference type="GeneID" id="1451334"/>
    <dbReference type="KEGG" id="mja:MJ_0472"/>
    <dbReference type="eggNOG" id="arCOG00781">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_3_1_2"/>
    <dbReference type="InParanoid" id="P54010"/>
    <dbReference type="OMA" id="HPSGYEE"/>
    <dbReference type="OrthoDB" id="110802at2157"/>
    <dbReference type="PhylomeDB" id="P54010"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_L32e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_L32e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_L32e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_L32e_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="PTHR23413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="SSF52042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000131151" description="50S ribosomal protein L32e">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="146" mass="17274" checksum="0347A94FF9BEB9A4" modified="1996-10-01" version="1">MNRLLRLRFKLKMKKPDFIRQEAHRHKRLGEKWRRPKGRHSKMRLKWKEKPPVVEIGYRSPKAVRGLHPSGLEDVLVYNVKDLEKLNPETQGARIASTVGKRKKIEIIIRARELGIRILNISEEKQEELLKLAEKQEAQEVNETNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>P54030</accession>
    <name>RL11_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl11</name>
        <name type="ordered locus">MJ0373</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64346">
        <property type="entry name" value="E64346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869872.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="5COL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.25 A"/>
        <property type="chains" value="A/B=1-161"/>
    </dbReference>
    <dbReference type="PDB" id="5D8H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="C=1-161"/>
    </dbReference>
    <dbReference type="PDB" id="5DAR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.90 A"/>
        <property type="chains" value="C/F=1-161"/>
    </dbReference>
    <dbReference type="PDBsum" id="5COL"/>
    <dbReference type="PDBsum" id="5D8H"/>
    <dbReference type="PDBsum" id="5DAR"/>
    <dbReference type="AlphaFoldDB" id="P54030"/>
    <dbReference type="SMR" id="P54030"/>
    <dbReference type="STRING" id="243232.MJ_0373"/>
    <dbReference type="EnsemblBacteria" id="AAB98362">
        <property type="protein sequence ID" value="AAB98362"/>
        <property type="gene ID" value="MJ_0373"/>
    </dbReference>
    <dbReference type="GeneID" id="1451230"/>
    <dbReference type="KEGG" id="mja:MJ_0373"/>
    <dbReference type="eggNOG" id="arCOG04372">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_4_0_2"/>
    <dbReference type="InParanoid" id="P54030"/>
    <dbReference type="OMA" id="CKQFNAK"/>
    <dbReference type="OrthoDB" id="98187at2157"/>
    <dbReference type="PhylomeDB" id="P54030"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_L11/L12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_L11/L12_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_L11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_L11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_L11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_L11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="PTHR11661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="SSF46906"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="SSF54747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104435" description="50S ribosomal protein L11">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="3"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="17"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="21"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="26"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="64"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="73"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="100"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="135"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="5COL"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5D8H"/>
        </source>
    </evidence>
    <sequence length="161" mass="17490" checksum="2B3B00332D110A81" modified="1996-10-01" version="1">MAKEVVEVLVTGGRATAGPPLGPAIGPLGVNVMQVVKEINEKTKDYEGMQVPVKVIVDTETRKFEIEVGIPPTTALIKKELGIETAAHEPRHEVVGNLTLEQVIKIAKMKKDAMLSYTLKNAVKEVLGTCGSMGVTVEGKDPKEVQKEIDAGVYDEYFKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>P54055</accession>
    <name>RS8E_METJA</name>
    <protein>
        <recommendedName>
            <fullName>30S ribosomal protein S8e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rps8e</name>
        <name type="ordered locus">MJ0673</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64384">
        <property type="entry name" value="A64384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870178.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54055"/>
    <dbReference type="SMR" id="P54055"/>
    <dbReference type="STRING" id="243232.MJ_0673"/>
    <dbReference type="EnsemblBacteria" id="AAB98667">
        <property type="protein sequence ID" value="AAB98667"/>
        <property type="gene ID" value="MJ_0673"/>
    </dbReference>
    <dbReference type="GeneID" id="1451539"/>
    <dbReference type="KEGG" id="mja:MJ_0673"/>
    <dbReference type="eggNOG" id="arCOG04154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080597_2_1_2"/>
    <dbReference type="InParanoid" id="P54055"/>
    <dbReference type="OMA" id="NPNYVRR"/>
    <dbReference type="OrthoDB" id="116294at2157"/>
    <dbReference type="PhylomeDB" id="P54055"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_S8e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_S8e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_S8e/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_S8e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="PTHR10394"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000122267" description="30S ribosomal protein S8e">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="129" mass="14539" checksum="1C51A1576A252E16" modified="1996-10-01" version="1">MSVWQGRSRRKPTGGLYRPARKKRKYEMGREPIETHVAEEAFKIKKVRTRGGNLKVKVVRTGFANVLDPETGTCKKVKIITVRENKANIHYVRRNVITKGAIIETEIGLAKVTSRPGQDGTVNAILIKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>P61159</accession>
    <name>FDHA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Formate dehydrogenase subunit alpha</fullName>
            <ecNumber>1.17.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fdhA</name>
        <name type="ordered locus">MJ1353</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="J. Mol. Biol." volume="266" first="637" last="641">
            <title>Selenoprotein synthesis in archaea: identification of an mRNA element of Methanococcus jannaschii probably directing selenocysteine insertion.</title>
            <authorList>
                <person name="Wilting R."/>
                <person name="Schorling S."/>
                <person name="Persson B.C."/>
                <person name="Boeck A."/>
            </authorList>
            <dbReference type="PubMed" id="9102456"/>
            <dbReference type="DOI" id="10.1006/jmbi.1996.0812"/>
        </citation>
        <scope>PROBABLE SELENOCYSTEINE AT SEC-131</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the oxidation of formate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>formate + NAD(+) = CO2 + NADH</text>
            <dbReference type="Rhea" id="RHEA:15985"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.17.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mo-bis(molybdopterin guanine dinucleotide)</name>
            <dbReference type="ChEBI" id="CHEBI:60539"/>
        </cofactor>
        <text evidence="1">Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Dimer of an alpha and a beta subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.9"/>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="InParanoid" id="P61159"/>
    <dbReference type="OMA" id="DGPPCCY"/>
    <dbReference type="PhylomeDB" id="P61159"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009326">
        <property type="term" value="C:formate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008863">
        <property type="term" value="F:formate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015942">
        <property type="term" value="P:formate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02790">
        <property type="entry name" value="MopB_CT_Formate-Dh_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02753">
        <property type="entry name" value="MopB_Formate-Dh-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041925">
        <property type="entry name" value="CT_Formate-Dh_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041924">
        <property type="entry name" value="Formate_Dh-H_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006478">
        <property type="entry name" value="Formate_DH_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006656">
        <property type="entry name" value="Mopterin_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006963">
        <property type="entry name" value="Mopterin_OxRdtase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006655">
        <property type="entry name" value="Mopterin_OxRdtase_prok_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027467">
        <property type="entry name" value="MopterinOxRdtase_cofactor_BS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04879">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00384">
        <property type="entry name" value="Molybdopterin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00926">
        <property type="entry name" value="Molybdop_Fe4S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="SSF50692"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01591">
        <property type="entry name" value="Fdh-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51669">
        <property type="entry name" value="4FE4S_MOW_BIS_MGD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00551">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00490">
        <property type="entry name" value="MOLYBDOPTERIN_PROK_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0500">Molybdenum</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0712">Selenocysteine</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000063226" description="Formate dehydrogenase subunit alpha">
        <location>
            <begin position="1"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S Mo/W bis-MGD-type" evidence="2">
        <location>
            <begin position="3"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="non-standard amino acid" description="Selenocysteine" evidence="3">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="673" mass="75770" checksum="B87A668BDF6B98D5" modified="2008-02-26" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>P81309</accession>
    <name>Y52A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0526.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0526.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH386.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81309"/>
    <dbReference type="SMR" id="P81309"/>
    <dbReference type="STRING" id="243232.MJ_0526.1"/>
    <dbReference type="DNASU" id="1451391"/>
    <dbReference type="EnsemblBacteria" id="AAB98527">
        <property type="protein sequence ID" value="AAB98527"/>
        <property type="gene ID" value="MJ_0526.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0526.1"/>
    <dbReference type="eggNOG" id="arCOG03190">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174513_1_0_2"/>
    <dbReference type="OMA" id="CINFCIA"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019214">
        <property type="entry name" value="DUF2109_membrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011316">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09882">
        <property type="entry name" value="DUF2109"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036534">
        <property type="entry name" value="EhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106916" description="Uncharacterized protein MJ0526.1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="82" mass="9169" checksum="4ABFA1488DE787EA" modified="1998-07-15" version="1">MMDIVEIIIGFIALLMTARIFLERSRARKLLYLCCLSFCISALIALYVDSPMGGIVAITYFICSTISSNAIAYTIEQTKHIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>P81329</accession>
    <name>ARTD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable archaeosortase D</fullName>
            <ecNumber evidence="1">3.4.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">artD</name>
        <name type="ordered locus">MJ1469.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="J. Bacteriol." volume="194" first="36" last="48">
            <title>Archaeosortases and exosortases are widely distributed systems linking membrane transit with posttranslational modification.</title>
            <authorList>
                <person name="Haft D.H."/>
                <person name="Payne S.H."/>
                <person name="Selengut J.D."/>
            </authorList>
            <dbReference type="PubMed" id="22037399"/>
            <dbReference type="DOI" id="10.1128/JB.06026-11"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>GENE FAMILY</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Transpeptidase that recognizes and modifies its substrate by proteolytic cleavage of a sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the archaeosortase and its substrate, which is then transferred and covalently attached to the cell membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the exosortase/archaeosortase family. Archaeosortase D subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870990.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81329"/>
    <dbReference type="STRING" id="243232.MJ_1469.1"/>
    <dbReference type="TCDB" id="9.B.297.3.1">
        <property type="family name" value="the archaeosortase/exosortase/rhomosortase (sortase) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99490">
        <property type="protein sequence ID" value="AAB99490"/>
        <property type="gene ID" value="MJ_1469.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1452374"/>
    <dbReference type="KEGG" id="mja:MJ_1469.1"/>
    <dbReference type="eggNOG" id="arCOG04471">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1599045_0_0_2"/>
    <dbReference type="OMA" id="TCSLEMA"/>
    <dbReference type="OrthoDB" id="124884at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026432">
        <property type="entry name" value="Archaeo_ArtD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04175">
        <property type="entry name" value="archaeo_artD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107355" description="Probable archaeosortase D">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="1">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="D4GUZ4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="22037399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="22037399"/>
        </source>
    </evidence>
    <sequence length="154" mass="18128" checksum="0CC1081E2F38B045" modified="1998-07-15" version="1">MGNKNAIYILRFLIYFFIFYYILKMLEGNIMDLLTITLSKLLNLKFYKNEIIVGKNIIEISSPCTCSLEMALFLGYIFGTPDVPIKYKISYSVFGLSIITISNILRIILIINYSNMINYNVVHDVISFIIFPIALFLNWFWIYLLKMKKIIMFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>P81330</accession>
    <name>Y13A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0139.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0139.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81330"/>
    <dbReference type="SMR" id="P81330"/>
    <dbReference type="STRING" id="243232.MJ_0139.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98131">
        <property type="protein sequence ID" value="AAB98131"/>
        <property type="gene ID" value="MJ_0139.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0139.1"/>
    <dbReference type="eggNOG" id="arCOG02134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2299352_0_0_2"/>
    <dbReference type="PhylomeDB" id="P81330"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106715" description="Uncharacterized protein MJ0139.1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <sequence length="100" mass="12094" checksum="8C7C2C24E8FEC1F3" modified="1998-06-01" version="1">MRLTIEVIKERIVERKLFKRNRKSIEVKILAGLLYYLGLSLRKVSLFLSQFEDISHESVRIYYHKIKEVLNRFPSNSKFDTVVSWIKSFMMFYNWVKSLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q57562</accession>
    <name>IF2B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">eif2b</name>
        <name type="ordered locus">MJ0097</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64312">
        <property type="entry name" value="A64312"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869589.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1K81">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=108-143"/>
    </dbReference>
    <dbReference type="PDB" id="1K8B">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=39-90"/>
    </dbReference>
    <dbReference type="PDBsum" id="1K81"/>
    <dbReference type="PDBsum" id="1K8B"/>
    <dbReference type="AlphaFoldDB" id="Q57562"/>
    <dbReference type="BMRB" id="Q57562"/>
    <dbReference type="SMR" id="Q57562"/>
    <dbReference type="STRING" id="243232.MJ_0097"/>
    <dbReference type="EnsemblBacteria" id="AAB98077">
        <property type="protein sequence ID" value="AAB98077"/>
        <property type="gene ID" value="MJ_0097"/>
    </dbReference>
    <dbReference type="GeneID" id="1450936"/>
    <dbReference type="KEGG" id="mja:MJ_0097"/>
    <dbReference type="eggNOG" id="arCOG01640">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026663_3_1_2"/>
    <dbReference type="InParanoid" id="Q57562"/>
    <dbReference type="OMA" id="GKPDTRL"/>
    <dbReference type="OrthoDB" id="96453at2157"/>
    <dbReference type="PhylomeDB" id="Q57562"/>
    <dbReference type="EvolutionaryTrace" id="Q57562"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="PTHR23001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="SSF100966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="SSF75689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00311">
        <property type="entry name" value="aIF-2beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137421" description="Translation initiation factor 2 subunit beta">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="46"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="52"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="75"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="84"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="117"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="126"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="136"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1K81"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1K8B"/>
        </source>
    </evidence>
    <sequence length="143" mass="16797" checksum="1762C47A953FEB25" modified="1997-11-01" version="1">MSDLENIDYYDYKALLKRARSQIPDYVFQKDRFELPEIEILIEGNRTIIRNFRELAKAVNRDEEFFAKYLLKETGSAGNLEGGRLILQRRISPELLKSRINDFLREYVICRECGKPDTKIIKEGRVHLLKCMACGAIRPIRMI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57575</accession>
    <name>SECD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein-export membrane protein SecD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">secD</name>
        <name type="ordered locus">MJ0111</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the protein translocation apparatus. Forms a complex with SecF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SecD/SecF family. SecD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64313">
        <property type="entry name" value="G64313"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869603.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57575"/>
    <dbReference type="SMR" id="Q57575"/>
    <dbReference type="STRING" id="243232.MJ_0111"/>
    <dbReference type="EnsemblBacteria" id="AAB98092">
        <property type="protein sequence ID" value="AAB98092"/>
        <property type="gene ID" value="MJ_0111"/>
    </dbReference>
    <dbReference type="GeneID" id="1450952"/>
    <dbReference type="KEGG" id="mja:MJ_0111"/>
    <dbReference type="eggNOG" id="arCOG03055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007894_5_1_2"/>
    <dbReference type="InParanoid" id="Q57575"/>
    <dbReference type="OMA" id="SAHLQMM"/>
    <dbReference type="OrthoDB" id="14000at2157"/>
    <dbReference type="PhylomeDB" id="Q57575"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01463_A">
        <property type="entry name" value="SecD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022646">
        <property type="entry name" value="SecD/SecF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024912">
        <property type="entry name" value="SecD_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PTHR30081"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07549">
        <property type="entry name" value="Sec_GG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0653">Protein transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0811">Translocation</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000095990" description="Protein-export membrane protein SecD">
        <location>
            <begin position="1"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01463"/>
        </source>
    </evidence>
    <sequence length="396" mass="43052" checksum="70BA0A62C74E9251" modified="1996-11-01" version="1">MDISKLLKDRKILILIIFVTLSVFLIVFKGLDFGIDLSGGTIIVLKAEKPMSDKEIEATIKIITERLNYNGLNDVVIYPRGNDEIIVEIPKSCDTDRIIKILKQQGVFVAKIDNITAYTGSDVQNVELPTKIPQGETWAYGVPFELTLEGAKKFAEVAKGKAYHKVELYMDGKLISAPVLSPDLADGKPHPQQVITVGAYPPTKEEIDEAMAIYSALKSGALPVKLDIEYISTISPEFGKEFLKGTAIALLLAFIAVGIIVSIRYKQPKIAIPILITCISEVIIILGFASLIDWKLDLPSIAGIIAAVGTGVDNQIVITDEALKRGAGKIRASIKRAFFIIFASAATSIAAMLPLFVLGVGMLKGFAITTIAGVLIGIFITRPAFARIIEEMFKKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57581</accession>
    <name>IF2A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">eif2a</name>
        <name type="ordered locus">MJ0117</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64314">
        <property type="entry name" value="E64314"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57581"/>
    <dbReference type="SMR" id="Q57581"/>
    <dbReference type="STRING" id="243232.MJ_0117"/>
    <dbReference type="EnsemblBacteria" id="AAB98098">
        <property type="protein sequence ID" value="AAB98098"/>
        <property type="gene ID" value="MJ_0117"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0117"/>
    <dbReference type="eggNOG" id="arCOG04107">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033458_0_2_2"/>
    <dbReference type="InParanoid" id="Q57581"/>
    <dbReference type="OMA" id="DVNEHQR"/>
    <dbReference type="PhylomeDB" id="Q57581"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00231">
        <property type="entry name" value="eIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022967">
        <property type="entry name" value="S1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022964">
        <property type="entry name" value="TIF2_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="PTHR10602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="SSF110993"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="SSF116742"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0396">Initiation factor</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000137394" description="Translation initiation factor 2 subunit alpha">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="S1 motif">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="266" mass="30485" checksum="31D2805A642D372A" modified="1997-11-01" version="1">MFIMRREFPEEGDIVIGTVKDVKPYGAFVELLEYPGKEGMIHISEVTSGWVKNIRDHVKVGQRVVAKVLRVDERKGHIDLSLKRVTEQQKRAKVQEWKRFQRASKMLERAAEKLGKSLEEAWEEVGYLLEDEFGELYNAFETMVIEGKEVLDDLEISEEWKNVLYEVAKESIELTNVEVEGVIEMKSYAPDGIKQIKKALTTALKANPYEDVEVKITYIGAPKYRVVVIAPDYKSGEEVFKKVCEKAVATIKKLGGEGTYYRESKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57594</accession>
    <name>T1S1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Type I restriction enzyme MjaVII specificity subunit</fullName>
            <shortName>S protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Type I specificity subunit S.MjaVII</fullName>
            <shortName evidence="3">S.MjaVII</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Type-1 restriction enzyme MjaXIP specificity subunit</fullName>
            <shortName>S.MjaXIP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0130</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <reference evidence="6" key="4">
        <citation type="journal article" date="2005" name="Proc. Natl. Acad. Sci. U.S.A." volume="102" first="3248" last="3253">
            <title>Crystal structure of DNA sequence specificity subunit of a type I restriction-modification enzyme and its functional implications.</title>
            <authorList>
                <person name="Kim J.-S."/>
                <person name="DeGiovanni A."/>
                <person name="Jancarik J."/>
                <person name="Adams P.D."/>
                <person name="Yokota H."/>
                <person name="Kim R."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="15728358"/>
            <dbReference type="DOI" id="10.1073/pnas.0409851102"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <scope>DOMAIN</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3 5">The specificity (S) subunit of a type I restriction enzyme; this subunit dictates DNA sequence specificity. The M and S subunits together form a methyltransferase (MTase) that methylates A-3 on the top and bottom strands of the sequence 5'-CAAN(7)TGG-3'. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated (PubMed:12654995) (Probable). After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="domain">
        <text evidence="2 5">Contains two target DNA recognition domains (TDR), each specifying recognition of one of the two defined components of the target sequence (Probable). The TDRs show high structural similarity and each forms a globular structure. The two TDRs are separated by 2 long, conserved antiparallel helices that form a coiled coil structure. These conserved regions may act as a molecular ruler for the separation between the two recognized DNA sequences (PubMed:15728358).</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg(2+) as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAB98112" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98112.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="1YF2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B=1-425"/>
    </dbReference>
    <dbReference type="PDBsum" id="1YF2"/>
    <dbReference type="AlphaFoldDB" id="Q57594"/>
    <dbReference type="SMR" id="Q57594"/>
    <dbReference type="STRING" id="243232.MJ_0130"/>
    <dbReference type="REBASE" id="154997">
        <property type="entry name" value="S.VscVS12ORF1031P"/>
    </dbReference>
    <dbReference type="REBASE" id="182833">
        <property type="entry name" value="S.Bli37I"/>
    </dbReference>
    <dbReference type="REBASE" id="191882">
        <property type="entry name" value="S2.Apa1468ORF2954P"/>
    </dbReference>
    <dbReference type="REBASE" id="203440">
        <property type="entry name" value="S3.Lpl434ORF2272P"/>
    </dbReference>
    <dbReference type="REBASE" id="203797">
        <property type="entry name" value="S.Ppe892ORF47P"/>
    </dbReference>
    <dbReference type="REBASE" id="203829">
        <property type="entry name" value="S.Bli141ORF4598P"/>
    </dbReference>
    <dbReference type="REBASE" id="203832">
        <property type="entry name" value="S.Bli27ORF807P"/>
    </dbReference>
    <dbReference type="REBASE" id="290906">
        <property type="entry name" value="S.Msa27082ORF3559P"/>
    </dbReference>
    <dbReference type="REBASE" id="3901">
        <property type="entry name" value="S.MjaVII"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98112">
        <property type="protein sequence ID" value="AAB98112"/>
        <property type="gene ID" value="MJ_0130"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0130"/>
    <dbReference type="eggNOG" id="arCOG02626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021095_10_3_2"/>
    <dbReference type="InParanoid" id="Q57594"/>
    <dbReference type="OMA" id="RMSITND"/>
    <dbReference type="PhylomeDB" id="Q57594"/>
    <dbReference type="EvolutionaryTrace" id="Q57594"/>
    <dbReference type="PRO" id="PR:Q57594"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
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    <evidence type="ECO:0000250" key="1">
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            <dbReference type="PDB" id="1YF2"/>
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    <sequence length="425" mass="48568" checksum="E196A947574FE91D" modified="2006-10-03" version="3">MFYKEENFKKTEIGEIPEDWEIVELKDVCKKIKAGGTPKTSVEEYYKNGTIPFVKIEDITNSNKYLTNTKIKITEEGLNNSNAWIVPKNSVLFAMYGSIGETAINKIEVATNQAILGIIPKDNILESEFLYYILAKNKNYYSKLGMQTTQKNLNAQIVKSFKIPLPPLEEQKQIAKILTKIDEGIEIIEKSINKLERIKKGLMHKLLTKGIGHSRFKKSEIGEIPEDWEVFEIKDIFEVKTGTTPSTKKSEYWENGEINWITPLDLSRLNEKIYIGSSERKVTKIALEKCNLNLIPKGSIIISTRAPVGYVAVLTVESTFNQGCKGLFQKNNDSVNTEFYAYYLKFKKNLLENLSGGSTFKELSKSMLENFKIPLPPLEEQKQIAKILSSVDKSIELKKQKKEKLQRMKKKIMELLLTGKVRVKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q57603</accession>
    <name>MVP_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Hyperpolarization-activated voltage-gated potassium channel</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>MVP</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mvp</name>
        <name type="ordered locus">MJ0139</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2003" name="Nat. Neurosci." volume="6" first="353" last="361">
            <title>Hyperpolarization moves S4 sensors inward to open MVP, a methanococcal voltage-gated potassium channel.</title>
            <authorList>
                <person name="Sesti F."/>
                <person name="Rajan S."/>
                <person name="Gonzalez-Colaso R."/>
                <person name="Nikolaeva N."/>
                <person name="Goldstein S.A."/>
            </authorList>
            <dbReference type="PubMed" id="12640457"/>
            <dbReference type="DOI" id="10.1038/nn1028"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="FEBS Lett." volume="547" first="165" last="169">
            <title>MjK1, a K+ channel from M. jannaschii, mediates K+ uptake and K+ sensitivity in E. coli.</title>
            <authorList>
                <person name="Hellmer J."/>
                <person name="Zeilinger C."/>
            </authorList>
            <dbReference type="PubMed" id="12860407"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(03)00706-3"/>
        </citation>
        <scope>FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Voltage-gated potassium-selective channel opened by hyperpolarization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cell membrane</location>
            <topology>Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text>The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Inhibited by barium.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the potassium channel family.</text>
    </comment>
    <dbReference type="EMBL" id="AY268105">
        <property type="protein sequence ID" value="AAP31055.1"/>
        <property type="molecule type" value="mRNA"/>
    </dbReference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C64317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869632.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="STRING" id="243232.MJ_0139"/>
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        <property type="family name" value="the voltage-gated ion channel (vic) superfamily"/>
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    <dbReference type="PRIDE" id="Q57603"/>
    <dbReference type="EnsemblBacteria" id="AAB98122">
        <property type="protein sequence ID" value="AAB98122"/>
        <property type="gene ID" value="MJ_0139"/>
    </dbReference>
    <dbReference type="GeneID" id="1450980"/>
    <dbReference type="KEGG" id="mja:MJ_0139"/>
    <dbReference type="eggNOG" id="arCOG01964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313152_0_0_2"/>
    <dbReference type="InParanoid" id="Q57603"/>
    <dbReference type="OrthoDB" id="91207at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071805">
        <property type="term" value="P:potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="PTHR11537"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0631">Potassium channel</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <keyword id="KW-0851">Voltage-gated channel</keyword>
    <feature type="chain" id="PRO_0000054098" description="Hyperpolarization-activated voltage-gated potassium channel">
        <location>
            <begin position="1"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Segment S1" evidence="2">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="32"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Segment S2" evidence="2">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="60"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Segment S3" evidence="2">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Voltage-sensor; Name=Segment S4" evidence="2">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="118"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Segment S5" evidence="2">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="147"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical; Name=Segment S6" evidence="2">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="203"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Selectivity filter" evidence="1">
        <location>
            <begin position="170"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12860407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="209" mass="24183" checksum="FC91E7320F34D90F" modified="1997-11-01" version="1">MNLKDRRLKKIMEVLSLIFTFEIVASFILSTYNPPYQDLLIKLDYISIMFFTFEFIYNFYYVEDKAKFFKDIYNIVDAIVVIAFLLYSLQVFYSKAFLGLRVINLLRILVLLRIIKLRKLEENQALINFLTLLTICFIASCLIWIVESGVNPAINNFFDAFYFTTISITTVGYGDITPKTDAGKLIIIFSVLFFISGLITSLQKALKGD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q57607</accession>
    <name>Y142_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative toxin MJ0142</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>UPF0332 protein MJ0142</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0142</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative toxin component of a putative type VII toxin-antitoxin (TA) system. Its cognate antitoxin might be MJ0141.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0332 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64317">
        <property type="entry name" value="G64317"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869637.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57607"/>
    <dbReference type="SMR" id="Q57607"/>
    <dbReference type="STRING" id="243232.MJ_0142"/>
    <dbReference type="EnsemblBacteria" id="AAB98125">
        <property type="protein sequence ID" value="AAB98125"/>
        <property type="gene ID" value="MJ_0142"/>
    </dbReference>
    <dbReference type="GeneID" id="1450986"/>
    <dbReference type="KEGG" id="mja:MJ_0142"/>
    <dbReference type="eggNOG" id="arCOG02123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151247_1_0_2"/>
    <dbReference type="InParanoid" id="Q57607"/>
    <dbReference type="OMA" id="YYTMFYC"/>
    <dbReference type="OrthoDB" id="118985at2157"/>
    <dbReference type="PhylomeDB" id="Q57607"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000159639" description="Putative toxin MJ0142">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="147" mass="17592" checksum="707D67BF058E065C" modified="1998-12-15" version="2">MRLKIMELRYKRELEKLIEKAEKSLEASENLYNSEFYDFAVSRIYYSMFYCVKALLLTKEINPKKHSGVLKMFAKEFIKTNELDVELFEYINEAYNYRQTADYDATIEIKKEEAEYLLHKGHIFLNKTKKYLISKNILKGENDNTKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q57628</accession>
    <name>Y164_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0164</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0164</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64320">
        <property type="entry name" value="E64320"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57628"/>
    <dbReference type="STRING" id="243232.MJ_0164"/>
    <dbReference type="EnsemblBacteria" id="AAB98152">
        <property type="protein sequence ID" value="AAB98152"/>
        <property type="gene ID" value="MJ_0164"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0164"/>
    <dbReference type="eggNOG" id="arCOG06658">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_712928_0_0_2"/>
    <dbReference type="OMA" id="IDVYEYN"/>
    <dbReference type="PhylomeDB" id="Q57628"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019249">
        <property type="entry name" value="DUF2226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09987">
        <property type="entry name" value="DUF2226"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106726" description="Uncharacterized protein MJ0164">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="395" mass="46274" checksum="5EA400ABDCC9A412" modified="1996-11-01" version="1">MRENMIKVVDGEYVKTLYEGNLEEIINEIDTGYILILVKEGNKLHEGYIFVEDGKIVGCYYTDSESTEVFGNKEKVIELLNYENKVIDIYKYNKDKINLMKWLYPEIFACKDTNKVSEKNEDMSEKRDIVEKYLNIKLDIPLDNLIEANTKDFEKYLEDNKYIIINAYRKKDGKFENGYIIYKGQTPIAAAYECDLGVLLGKDAYEKLEEMLKDENTVIDVYEYNEKKTHVILELYPQMKILDENENKSSEKADSLESEGSITTAEEIEEESTVSREELLKKLGIKEPDENWIETILEDVFRPSDEELEELKEKIESEIVNKVKRMEGVSDVLVNLKIKWENGRYYIFGDVNVKRKRILGIIKKDIDPSIVKFEIDNTIKKYVSKYTSRININIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57633</accession>
    <name>Y169_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0169</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0169</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ParA family. MinD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64321">
        <property type="entry name" value="B64321"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57633"/>
    <dbReference type="SMR" id="Q57633"/>
    <dbReference type="PRIDE" id="Q57633"/>
    <dbReference type="EnsemblBacteria" id="AAB98154">
        <property type="protein sequence ID" value="AAB98154"/>
        <property type="gene ID" value="MJ_0169"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0169"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037612_0_3_2"/>
    <dbReference type="InParanoid" id="Q57633"/>
    <dbReference type="OMA" id="KDMAIHL"/>
    <dbReference type="PhylomeDB" id="Q57633"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106728" description="Uncharacterized ATP-binding protein MJ0169">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q72H90"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="263" mass="28950" checksum="292FBE38EE72ACF4" modified="1996-11-01" version="1">MIFMIITIASGKGGVGKTTTSASLAVALAKLGKKVLAIDGDISMANLGILFNMEKKKPSLHEVLSEEADVRDAIYKHKTGVYVLPTSLSLEGYKKSDIDLLPDVVNEVADDFDYVIIDAPAGLNREMATHLAIADKLLLVVTPEMFSIIDAVRLKESAEMAGTPLMGVVLNRVGRDFGEMGRDEIEMLIKGKVLVEVPEDENVRSAALKKMSVIEYRKNSPASQAYMKLASIIAGVPIYIEDEIKIIRKESFIDKIKRLFRMY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q57636</accession>
    <name>PIMT_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber>2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Protein-beta-aspartate methyltransferase</fullName>
            <shortName>PIMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pcm</name>
        <name type="ordered locus">MJ0172</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64321">
        <property type="entry name" value="E64321"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869667.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2YXE">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=1-215"/>
    </dbReference>
    <dbReference type="PDBsum" id="2YXE"/>
    <dbReference type="AlphaFoldDB" id="Q57636"/>
    <dbReference type="SMR" id="Q57636"/>
    <dbReference type="STRING" id="243232.MJ_0172"/>
    <dbReference type="PRIDE" id="Q57636"/>
    <dbReference type="EnsemblBacteria" id="AAB98157">
        <property type="protein sequence ID" value="AAB98157"/>
        <property type="gene ID" value="MJ_0172"/>
    </dbReference>
    <dbReference type="GeneID" id="1451019"/>
    <dbReference type="KEGG" id="mja:MJ_0172"/>
    <dbReference type="eggNOG" id="arCOG00976">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055432_2_0_2"/>
    <dbReference type="InParanoid" id="Q57636"/>
    <dbReference type="OMA" id="TISAIHM"/>
    <dbReference type="OrthoDB" id="88476at2157"/>
    <dbReference type="PhylomeDB" id="Q57636"/>
    <dbReference type="EvolutionaryTrace" id="Q57636"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00090">
        <property type="entry name" value="PIMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PTHR11579"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000111916" description="Protein-L-isoaspartate O-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="3"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="23"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="40"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="52"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="58"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="80"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="103"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="112"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="130"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="161"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2YXE"/>
        </source>
    </evidence>
    <sequence length="215" mass="23761" checksum="E5A8634F2E2BAE8B" modified="1996-11-01" version="1">MDLEEQKKAVIEKLIREGYIKSKRVIDALLKVPREEFLPEHLKEYAYVDTPLEIGYGQTISAIHMVGMMCELLDLKPGMKVLEIGTGCGYHAAVTAEIVGEDGLVVSIERIPELAEKAERTLRKLGYDNVIVIVGDGTLGYEPLAPYDRIYTTAAGPKIPEPLIRQLKDGGKLLMPVGRYLQRLVLAEKRGDEIIIKDCGPVAFVPLVGKEGFQG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q57639</accession>
    <name>Y175_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0175</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0175</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64321">
        <property type="entry name" value="H64321"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57639"/>
    <dbReference type="SMR" id="Q57639"/>
    <dbReference type="STRING" id="243232.MJ_0175"/>
    <dbReference type="EnsemblBacteria" id="AAB98160">
        <property type="protein sequence ID" value="AAB98160"/>
        <property type="gene ID" value="MJ_0175"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0175"/>
    <dbReference type="eggNOG" id="arCOG04318">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099315_0_0_2"/>
    <dbReference type="InParanoid" id="Q57639"/>
    <dbReference type="OMA" id="EKTHGDI"/>
    <dbReference type="PhylomeDB" id="Q57639"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016070">
        <property type="term" value="P:RNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033956">
        <property type="entry name" value="Translin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="PTHR10741"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741:SF2">
        <property type="entry name" value="PTHR10741:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="SSF74784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106729" description="Uncharacterized protein MJ0175">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <sequence length="222" mass="26426" checksum="9FBBB385C9FFD3D3" modified="1996-11-01" version="1">MYKQILTIGYIKFLVVAMDELNYLINYLANKDSVREEILKLSREITRDCAMLIRKIHKSDDKDEFKDKLNEISEKIKKLNSLATFPEFVGYLSTPQQEFVEALSLYMIKFDNKIPSFKELDFIKEENYILGLADVIGELRREVLEAMKNDNLAEVERYFKFMEDLYEFLMNFDYYHVVDNLRRKQDISRGILEKTHGDIVTFIQNLKLREHLKRVQIGLSQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57651</accession>
    <name>Y198_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0198</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0198</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G64324"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869693.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57651"/>
    <dbReference type="SMR" id="Q57651"/>
    <dbReference type="STRING" id="243232.MJ_0198"/>
    <dbReference type="EnsemblBacteria" id="AAB98193">
        <property type="protein sequence ID" value="AAB98193"/>
        <property type="gene ID" value="MJ_0198"/>
    </dbReference>
    <dbReference type="GeneID" id="1451047"/>
    <dbReference type="KEGG" id="mja:MJ_0198"/>
    <dbReference type="eggNOG" id="arCOG01170">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_893150_0_0_2"/>
    <dbReference type="OMA" id="IDSYLWE"/>
    <dbReference type="OrthoDB" id="39216at2157"/>
    <dbReference type="PhylomeDB" id="Q57651"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="SSF54826"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106737" description="Uncharacterized protein MJ0198">
        <location>
            <begin position="1"/>
            <end position="314"/>
        </location>
    </feature>
    <sequence length="314" mass="35242" checksum="439FC94A7DAEDDEA" modified="1996-11-01" version="1">MTNVIIEKISAKEVFKGAKIKVMITTMTNISIGYDIIEVNNPEEAIADVENVIAPELIGYPATDIDFIDSLICETSVNNPTVAMGISISVARAASNSLDIPLFKFLGGALTTELPIVASGILVDKDKNELIPIVMADSIEDIVNLYLKLTDVLSHDYSIVNIDGAYTCKDIFNEIPKIRNLIDEIKEDEDLDILLGLSSKKETVKDKDLSQIDYLEVEEPVEFDGFLCTDSIYEESDFVKVFPYEMGTITEMYYYINYIMDKGLYPVIFGNNSSFAHIAVSFKVPFLRPKLSSNVLNEVWNIERTIMNPNIRRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q57664</accession>
    <name>GALE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative UDP-glucose 4-epimerase</fullName>
            <ecNumber>5.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Galactowaldenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>UDP-galactose 4-epimerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0211</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64326">
        <property type="entry name" value="D64326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869707.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57664"/>
    <dbReference type="SMR" id="Q57664"/>
    <dbReference type="STRING" id="243232.MJ_0211"/>
    <dbReference type="PRIDE" id="Q57664"/>
    <dbReference type="EnsemblBacteria" id="AAB98196">
        <property type="protein sequence ID" value="AAB98196"/>
        <property type="gene ID" value="MJ_0211"/>
    </dbReference>
    <dbReference type="GeneID" id="1451061"/>
    <dbReference type="KEGG" id="mja:MJ_0211"/>
    <dbReference type="eggNOG" id="arCOG01369">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_7_2"/>
    <dbReference type="InParanoid" id="Q57664"/>
    <dbReference type="OMA" id="GEHLICN"/>
    <dbReference type="OrthoDB" id="43299at2157"/>
    <dbReference type="PhylomeDB" id="Q57664"/>
    <dbReference type="UniPathway" id="UPA00214"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0299">Galactose metabolism</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000183225" description="Putative UDP-glucose 4-epimerase">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="30"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="50"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="183"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="198"/>
            <end position="200"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="263"/>
            <end position="266"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="305" mass="34542" checksum="2756773CF95D50BD" modified="1996-11-01" version="1">MILVTGGAGFIGSHIVDKLIENNYDVIILDNLTTGNKNNINPKAEFVNADIRDKDLDEKINFKDVEVVIHQAAQINVRNSVENPVYDGDINVLGTINILEMMRKYDIDKIVFASSGGAVYGEPNYLPVDENHPINPLSPYGLSKYVGEEYIKLYNRLYGIEYAILRYSNVYGERQDPKGEAGVISIFIDKMLKNQSPIIFGDGNQTRDFVYVGDVAKANLMALNWKNEIVNIGTGKETSVNELFDIIKHEIGFRGEAIYDKPREGEVYRIYLDIKKAESLGWKPEIDLKEGIKRVVNWMKNNNRT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q57685</accession>
    <name>Y233_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0233</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0233</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ1189.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64329">
        <property type="entry name" value="B64329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869731.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57685"/>
    <dbReference type="STRING" id="243232.MJ_0233"/>
    <dbReference type="EnsemblBacteria" id="AAB98226">
        <property type="protein sequence ID" value="AAB98226"/>
        <property type="gene ID" value="MJ_0233"/>
    </dbReference>
    <dbReference type="GeneID" id="1451086"/>
    <dbReference type="KEGG" id="mja:MJ_0233"/>
    <dbReference type="eggNOG" id="arCOG02880">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083802_0_0_2"/>
    <dbReference type="OMA" id="PEWANYW"/>
    <dbReference type="OrthoDB" id="119187at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106753" description="Uncharacterized protein MJ0233">
        <location>
            <begin position="1"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="243"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="277" mass="31307" checksum="31F66D38BD552B39" modified="1996-11-01" version="1">MGTIESYLTNSYNYIIFNFKKLCIGGLMSAIVGAMSGVTTAFIDLFMERANYDIMHIIMSFLIYFGIIFIIGLIVSAIIGGYNVRIMKTTVEGLNVAPDWNNITDLLYRGILYIVGLVLLNIIFYFIPAILFVFGIFSLYISKIIGAFLIIISILIFIISVISLWLYSKLAEVNYSVKGFYGFFEFKEIFKMIGIRYIILVIIIAIINFIISLIVVLPLNIIDIFISYSALANSILTIIYISIKGISYALSTFVDFYLGVFSIRAVALYYKDRKGIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q57708</accession>
    <name>Y260_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0260</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0260</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64332">
        <property type="entry name" value="E64332"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57708"/>
    <dbReference type="STRING" id="243232.MJ_0260"/>
    <dbReference type="EnsemblBacteria" id="AAB98246">
        <property type="protein sequence ID" value="AAB98246"/>
        <property type="gene ID" value="MJ_0260"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0260"/>
    <dbReference type="eggNOG" id="arCOG01739">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118895_0_0_2"/>
    <dbReference type="InParanoid" id="Q57708"/>
    <dbReference type="OMA" id="SAHWTNA"/>
    <dbReference type="PhylomeDB" id="Q57708"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027245">
        <property type="entry name" value="Sec11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="PTHR10806"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="PTHR10806:SF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="SSF51306"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106762" description="Uncharacterized protein MJ0260">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="203" mass="23724" checksum="9A6DC1DE1D8DF8C8" modified="1996-11-01" version="1">MVVLFLIWSHVNVVVSDSMYPIMKRGDLVIVENAGFEFNPNDVDVGDIVVYKAHWPYYQYLLSEIDYKLNLNPYTTLYIFKEGDFKDMSVKVLGEIKTDKSSYKILEADIPKSPTKPVIHRVIDKVEFNNKTYFIIKGDNNPIHDPELVSINQIKQRVIVVDGHPLVIPYVGYLSIWLKEYWYLVVLFVLIYYAYNYLKGGRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57739</accession>
    <name>FTSY_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Signal recognition particle receptor FtsY</fullName>
            <shortName evidence="1">SRP receptor</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftsY</name>
        <name type="ordered locus">MJ0291</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Acts as a receptor for the complex formed by the signal recognition particle (SRP) and the ribosome-nascent chain (RNC).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cell membrane</location>
            <topology>Peripheral membrane protein</topology>
            <orientation>Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP-binding SRP family. FtsY subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64336">
        <property type="entry name" value="D64336"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869789.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57739"/>
    <dbReference type="SMR" id="Q57739"/>
    <dbReference type="STRING" id="243232.MJ_0291"/>
    <dbReference type="EnsemblBacteria" id="AAB98276">
        <property type="protein sequence ID" value="AAB98276"/>
        <property type="gene ID" value="MJ_0291"/>
    </dbReference>
    <dbReference type="GeneID" id="1451146"/>
    <dbReference type="KEGG" id="mja:MJ_0291"/>
    <dbReference type="eggNOG" id="arCOG01227">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_3_1_2"/>
    <dbReference type="InParanoid" id="Q57739"/>
    <dbReference type="OMA" id="GISDQFQ"/>
    <dbReference type="OrthoDB" id="90641at2157"/>
    <dbReference type="PhylomeDB" id="Q57739"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031226">
        <property type="term" value="C:intrinsic component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="SSF47364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0675">Receptor</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000101218" description="Signal recognition particle receptor FtsY">
        <location>
            <begin position="1"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="209"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="291"/>
            <end position="295"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="349"/>
            <end position="352"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00920"/>
        </source>
    </evidence>
    <sequence length="409" mass="45755" checksum="4FBCFF8290693EEC" modified="1996-11-01" version="1">MFGKLKEKLLETASKITEKIYSKGEAEEVKEEKEEKSKISFTSLFKKEPKKEEVKKEKAEEEIKKSEIVKTEPSEKVEEAKEEIKEIKEEKEEKKITFFDRFGLTRAIKKVLKKEVVILEEDIEDVLEELEIALLEADVALEVVEKLIENIKNELVGRKISPDDNVEEITINAVKNAIKNILSQEKIDIEEIIKKNKAEGKPTVIVFVGINGTGKTTTIAKLAYKLKQKGYSVVLAAGDTFRAGAIEQLEQHAKNVGVKVIKHKPGADSAAVIYDAIQHAKARGIDVVLADTAGRQATNVNLMEEIKKVVRVTKPDLVIFVGDALTGNDAVYQAEEFNRAVNIDGIILTKVDADAKGGAALSIGYAIGKPILYLGVGQRYQDLIEFDADWMVRKLFGEEEFEFSTEREF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57773</accession>
    <name>Y327_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0327</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0327</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64340">
        <property type="entry name" value="G64340"/>
    </dbReference>
    <dbReference type="PDB" id="2KLA">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-104"/>
    </dbReference>
    <dbReference type="PDB" id="2QTD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=1-104"/>
    </dbReference>
    <dbReference type="PDBsum" id="2KLA"/>
    <dbReference type="PDBsum" id="2QTD"/>
    <dbReference type="AlphaFoldDB" id="Q57773"/>
    <dbReference type="BMRB" id="Q57773"/>
    <dbReference type="SMR" id="Q57773"/>
    <dbReference type="STRING" id="243232.MJ_0327"/>
    <dbReference type="EnsemblBacteria" id="AAB98315">
        <property type="protein sequence ID" value="AAB98315"/>
        <property type="gene ID" value="MJ_0327"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0327"/>
    <dbReference type="eggNOG" id="arCOG02734">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104194_2_3_2"/>
    <dbReference type="InParanoid" id="Q57773"/>
    <dbReference type="OMA" id="FLIFKIE"/>
    <dbReference type="PhylomeDB" id="Q57773"/>
    <dbReference type="EvolutionaryTrace" id="Q57773"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003731">
        <property type="entry name" value="Di-Nase_FeMo-co_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036105">
        <property type="entry name" value="DiNase_FeMo-co_biosyn_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02579">
        <property type="entry name" value="Nitro_FeMo-Co"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53146">
        <property type="entry name" value="SSF53146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106796" description="Uncharacterized protein MJ0327">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="4"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="23"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="45"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="57"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="66"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="77"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="86"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="2QTD"/>
        </source>
    </evidence>
    <sequence length="104" mass="11898" checksum="519531CD7ACC8741" modified="1996-11-01" version="1">MINMKVAISMDVDKISNSFEDCKYFLIVRIDDNEVKSTKVIFNDESGKKSIVKENVNAIICKNISEENYKKFSKKIEIYHAEGDDVDKNISLFIEGELSKISNP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57778</accession>
    <name>Y332_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0332</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0332</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64341">
        <property type="entry name" value="D64341"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869829.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57778"/>
    <dbReference type="SMR" id="Q57778"/>
    <dbReference type="STRING" id="243232.MJ_0332"/>
    <dbReference type="EnsemblBacteria" id="AAB98320">
        <property type="protein sequence ID" value="AAB98320"/>
        <property type="gene ID" value="MJ_0332"/>
    </dbReference>
    <dbReference type="GeneID" id="1451187"/>
    <dbReference type="KEGG" id="mja:MJ_0332"/>
    <dbReference type="eggNOG" id="arCOG09638">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257357_0_0_2"/>
    <dbReference type="OMA" id="KENQRTH"/>
    <dbReference type="OrthoDB" id="136986at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106801" description="Uncharacterized protein MJ0332">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <sequence length="110" mass="12180" checksum="88EA0EBA982A3301" modified="1996-11-01" version="1">MGVSMSLWKKLDEKENQRTHNLSNSDANDRILRVINSAFGVPDDIKEEVIAAIDKAIKHGLENGTLNYLKVIELACEGYTKEDIAEAYGHQILGAYVAVLILTGKPLKLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q57784</accession>
    <name>Y338_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0338</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0338</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64342">
        <property type="entry name" value="B64342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869836.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57784"/>
    <dbReference type="SMR" id="Q57784"/>
    <dbReference type="STRING" id="243232.MJ_0338"/>
    <dbReference type="EnsemblBacteria" id="AAB98326">
        <property type="protein sequence ID" value="AAB98326"/>
        <property type="gene ID" value="MJ_0338"/>
    </dbReference>
    <dbReference type="GeneID" id="1451194"/>
    <dbReference type="KEGG" id="mja:MJ_0338"/>
    <dbReference type="eggNOG" id="arCOG09643">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2257358_0_0_2"/>
    <dbReference type="OMA" id="IAILACY"/>
    <dbReference type="OrthoDB" id="136861at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106808" description="Uncharacterized protein MJ0338">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <sequence length="104" mass="11842" checksum="12C9316CFCC498E0" modified="1996-11-01" version="1">MIFMVDVSEILAKMKPLLGMSADEEFANPEQAEASANLAIKTSQVEDVDIIAILACYYYTESRDMKTDENVSVMANHFYRMYQIAIEEAKQKELNEEEGAVFFD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q57792</accession>
    <name>Y346_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0346</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0346</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64343">
        <property type="entry name" value="B64343"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869844.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57792"/>
    <dbReference type="STRING" id="243232.MJ_0346"/>
    <dbReference type="EnsemblBacteria" id="AAB98336">
        <property type="protein sequence ID" value="AAB98336"/>
        <property type="gene ID" value="MJ_0346"/>
    </dbReference>
    <dbReference type="GeneID" id="1451202"/>
    <dbReference type="KEGG" id="mja:MJ_0346"/>
    <dbReference type="HOGENOM" id="CLU_2285051_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106816" description="Uncharacterized protein MJ0346">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <sequence length="101" mass="11750" checksum="A467A4DB2728BF87" modified="1996-11-01" version="1">MEDMITLTDERKTVKFENLPEIYVRVHDEAKGLVLTVKNPYDTDVYAKVYYAPDGENYDTVTSEVVKYDYQAQKTELIPIKHPYMKISFDSAVSGWVSYIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q57797</accession>
    <name>Y351_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0351</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0351</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64343">
        <property type="entry name" value="G64343"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57797"/>
    <dbReference type="STRING" id="243232.MJ_0351"/>
    <dbReference type="EnsemblBacteria" id="AAB98340">
        <property type="protein sequence ID" value="AAB98340"/>
        <property type="gene ID" value="MJ_0351"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0351"/>
    <dbReference type="HOGENOM" id="CLU_1736427_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106822" description="Uncharacterized protein MJ0351">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <sequence length="150" mass="16810" checksum="8A82F7D62AA4D58B" modified="1996-11-01" version="1">MVEKMSPKTFGGEIMENSDIRAKLLEVLESGVVNGKWTIGIVRLNSELRRLLVNRCQCYINDSETTKSKTKIIAEFCMDAAENGVRYSPDLVNAALTILKSMGYNIKDVQCYYDVDFPAVITVDGEVGVVIKPEHMEDLRVVGVKKLTEF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q57830</accession>
    <name>CAS8A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated protein Cas8a2/Csa4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR-associated protein Cas8a2/Csa4, subtype I-A/Apern</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cas8a2</name>
        <name type="ordered locus">MJ0385</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas8a2/Csa4 family. Subtype I-A/Apern subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64348">
        <property type="entry name" value="A64348"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57830"/>
    <dbReference type="STRING" id="243232.MJ_0385"/>
    <dbReference type="EnsemblBacteria" id="AAB98380">
        <property type="protein sequence ID" value="AAB98380"/>
        <property type="gene ID" value="MJ_0385"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0385"/>
    <dbReference type="eggNOG" id="arCOG01441">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_722832_0_0_2"/>
    <dbReference type="InParanoid" id="Q57830"/>
    <dbReference type="OMA" id="KLMDFLW"/>
    <dbReference type="PhylomeDB" id="Q57830"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09666">
        <property type="entry name" value="Cas8a2_I-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010184">
        <property type="entry name" value="CRISPR-assoc_prot_MJ0385"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09703">
        <property type="entry name" value="Cas_Csa4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01914">
        <property type="entry name" value="cas_Csa4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106848" description="CRISPR-associated protein Cas8a2/Csa4">
        <location>
            <begin position="1"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="375" mass="43187" checksum="9A2853EA2CBEBE51" modified="1996-11-01" version="1">MIIKGLRVEKEKLQSLQKFWRLRVMLFETPGYNEILDLYIAYGVVECLVREGVENLRFFPIGNKYCIVVEEPTNVNIKNRIEKGMLNALEDMLSLHKAIGKYISTKEDIKIISDVDFSAGANINNVYWDGIPKTLEKIKENIKKGKLSTKSKNTVPLTLMPAAGKYMPKIYGVKGGNPIKIDDSNYALAWIGFHYYAPYINISDNRATYIHIYAIKPLEELGLIEILALKDLKKKINNYQLGKYKFFVNKKLALLYHLTHTESISALEIVTKKNFSVVSYTLENVDNNQAIRSFGEYDLSKLMDFLWYLKATDFYNTIQFVDNILRGDLEVSLTFIDGILYDDLDAVYSAIRKLKSVRISPLIIQSILEWFGNFY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q57835</accession>
    <name>Y390_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0390</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0390</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64348">
        <property type="entry name" value="F64348"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869889.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57835"/>
    <dbReference type="STRING" id="243232.MJ_0390"/>
    <dbReference type="EnsemblBacteria" id="AAB98381">
        <property type="protein sequence ID" value="AAB98381"/>
        <property type="gene ID" value="MJ_0390"/>
    </dbReference>
    <dbReference type="GeneID" id="1451247"/>
    <dbReference type="KEGG" id="mja:MJ_0390"/>
    <dbReference type="eggNOG" id="arCOG08287">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056049_0_0_2"/>
    <dbReference type="OMA" id="YVDIYLG"/>
    <dbReference type="OrthoDB" id="115839at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106851" description="Uncharacterized protein MJ0390">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <sequence length="130" mass="14973" checksum="5854471834A81D73" modified="1996-11-01" version="1">MEEKIILSIQNPEDVLISYVDIYLGDKNVSLEVLSKDTAKINLPFDKDEGEGEIVVKIKYKTLPHYKNNNNKKEVKKQDYKNLTQTLNEITKKTTNRKDNDIIIADSKPVSLDGLKKEEKKKKLNDIIIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q57865</accession>
    <name>DAPB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <shortName evidence="1">HTPA reductase</shortName>
            <ecNumber evidence="1">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapB</name>
        <name type="ordered locus">MJ0422</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98410" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98410.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64352">
        <property type="entry name" value="F64352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496490.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57865"/>
    <dbReference type="SMR" id="Q57865"/>
    <dbReference type="STRING" id="243232.MJ_0422"/>
    <dbReference type="EnsemblBacteria" id="AAB98410">
        <property type="protein sequence ID" value="AAB98410"/>
        <property type="gene ID" value="MJ_0422"/>
    </dbReference>
    <dbReference type="GeneID" id="1451282"/>
    <dbReference type="KEGG" id="mja:MJ_0422"/>
    <dbReference type="eggNOG" id="arCOG04393">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047479_2_1_2"/>
    <dbReference type="InParanoid" id="Q57865"/>
    <dbReference type="OMA" id="HHPNKAD"/>
    <dbReference type="OrthoDB" id="86833at2157"/>
    <dbReference type="PhylomeDB" id="Q57865"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00018"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016726">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00102">
        <property type="entry name" value="DapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="PTHR20836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141518" description="4-hydroxy-tetrahydrodipicolinate reductase">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="170"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>(S)-2,3,4,5-tetrahydrodipicolinate</name>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="274" mass="29955" checksum="D943FB6CF3DC706E" modified="2000-05-30" version="2">MIKVAVTGALGRMGSNIIKTITQQEDMKVVCAFEVPNHPKKGEDVGELIGIGKIGVPLSTADELDKVLKETKPDVLVDFTIAHACVENVKIAAKNGVNLVIGTTGFTEEQKAEIEKAIKENNVAAVISQNFAIGVNIFFKTLEFLAKKLGDYDIEIIEMHHRYKKDAPSGTALRAAEIIKANRGIESVFVYGRYGMTGERKKEEIGIHALRGGDVVGDHTVIFAGDGERIELTHRASSRQAFVNGVILAIRYIADKKEGIYNTFDVLGLNEIKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57953</accession>
    <name>FERA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0533</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0533</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64366">
        <property type="entry name" value="E64366"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870037.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57953"/>
    <dbReference type="SMR" id="Q57953"/>
    <dbReference type="STRING" id="243232.MJ_0533"/>
    <dbReference type="EnsemblBacteria" id="AAB98524">
        <property type="protein sequence ID" value="AAB98524"/>
        <property type="gene ID" value="MJ_0533"/>
    </dbReference>
    <dbReference type="GeneID" id="1451398"/>
    <dbReference type="KEGG" id="mja:MJ_0533"/>
    <dbReference type="eggNOG" id="arCOG00958">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_11_4_2"/>
    <dbReference type="InParanoid" id="Q57953"/>
    <dbReference type="OMA" id="CISCRIC"/>
    <dbReference type="OrthoDB" id="123760at2157"/>
    <dbReference type="PhylomeDB" id="Q57953"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159138" description="Uncharacterized ferredoxin MJ0533">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="2"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="56" mass="6160" checksum="A2DD7651E00B2AC1" modified="1996-11-01" version="1">MVKIDYKKCGYCGACVGVCEKLAINLIEHIIVIDEKKCNNCKLCTIVCPLNALEGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57963</accession>
    <name>RL10E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L10e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl10e</name>
        <name type="ordered locus">MJ0543</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64367">
        <property type="entry name" value="G64367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870047.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57963"/>
    <dbReference type="SMR" id="Q57963"/>
    <dbReference type="STRING" id="243232.MJ_0543"/>
    <dbReference type="EnsemblBacteria" id="AAB98535">
        <property type="protein sequence ID" value="AAB98535"/>
        <property type="gene ID" value="MJ_0543"/>
    </dbReference>
    <dbReference type="GeneID" id="1451408"/>
    <dbReference type="KEGG" id="mja:MJ_0543"/>
    <dbReference type="eggNOG" id="arCOG04113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084051_0_2_2"/>
    <dbReference type="InParanoid" id="Q57963"/>
    <dbReference type="OMA" id="HHVIREN"/>
    <dbReference type="OrthoDB" id="82104at2157"/>
    <dbReference type="PhylomeDB" id="Q57963"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_L10e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_L10e/L16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_L10e/L16_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_L10e_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_L10e_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="PTHR11726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="SSF54686"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000147135" description="50S ribosomal protein L10e">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <sequence length="174" mass="19576" checksum="CB9BB339DA713968" modified="1996-11-01" version="1">MASLRPNRCYRDVDKPPYTRKEYVKGVPQPKVVHFIMGNLSAEFPVKVNLVATRPIQIRHNALEAARVAANKYLTKMCGRMGYKFQIRVYPHQILREHKMATGAGADRISDGMRLAFGKPIGTAARVKEGQAILTVWVNPDKFPAAKEALRRAAMKLPVPCRIVIEQGKELLKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q57968</accession>
    <name>Y548_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0548</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0548</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=1-293"/>
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        <property type="resolution" value="3.05 A"/>
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    <dbReference type="EnsemblBacteria" id="AAB98540">
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
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    </evidence>
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</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58041</accession>
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        <recommendedName>
            <fullName>Uncharacterized ferredoxin MJ0624</fullName>
        </recommendedName>
    </protein>
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    <organism>
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        <lineage>
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        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <text evidence="1">Ferredoxins are iron-sulfur proteins that transfer electrons probably in the CO-dehydrogenase complex.</text>
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        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
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        </cofactor>
        <text evidence="1">Binds 2 [4Fe-4S] clusters.</text>
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    </dbReference>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
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</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58061</accession>
    <name>Y645_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0645</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0645</name>
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    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
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        <lineage>
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    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64380">
        <property type="entry name" value="E64380"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58061"/>
    <dbReference type="SMR" id="Q58061"/>
    <dbReference type="STRING" id="243232.MJ_0645"/>
    <dbReference type="EnsemblBacteria" id="AAB98639">
        <property type="protein sequence ID" value="AAB98639"/>
        <property type="gene ID" value="MJ_0645"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0645"/>
    <dbReference type="eggNOG" id="arCOG01091">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111441_1_0_2"/>
    <dbReference type="InParanoid" id="Q58061"/>
    <dbReference type="OMA" id="RETMICG"/>
    <dbReference type="PhylomeDB" id="Q58061"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005381">
        <property type="term" value="F:iron ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:cellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006880">
        <property type="term" value="P:intracellular sequestering of iron ion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006682">
        <property type="entry name" value="CHP00267"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="PTHR31851"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00267">
        <property type="entry name" value="TIGR00267"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106970" description="Uncharacterized protein MJ0645">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="24"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="191" mass="20046" checksum="7FB368CC3B48FE2F" modified="1996-11-01" version="1">MLRIPRSLKSIINTINGESGTRYIVRGLIDGSLSALGVVIGASGSADASVIIAAGLGGGIANGLSNILGAFTAEKASLERERIQKEKSLLKKNGYLKKSIIYKKAIRETMICGLIDGISTTIGSALPVVPFFLFDIKTALYVAIGITIAILFILGVFIGKISKENVIISGIKMVAGALAVAILCFMIEKAF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58076</accession>
    <name>Y662_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0662</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0662</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64382">
        <property type="entry name" value="F64382"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870167.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58076"/>
    <dbReference type="STRING" id="243232.MJ_0662"/>
    <dbReference type="EnsemblBacteria" id="AAB98659">
        <property type="protein sequence ID" value="AAB98659"/>
        <property type="gene ID" value="MJ_0662"/>
    </dbReference>
    <dbReference type="GeneID" id="1451528"/>
    <dbReference type="KEGG" id="mja:MJ_0662"/>
    <dbReference type="eggNOG" id="arCOG03951">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1318521_0_0_2"/>
    <dbReference type="InParanoid" id="Q58076"/>
    <dbReference type="OMA" id="WGGLMWY"/>
    <dbReference type="OrthoDB" id="57852at2157"/>
    <dbReference type="PhylomeDB" id="Q58076"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026841">
        <property type="entry name" value="Aur1/Ipt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxi_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="P_Acid_Pase_2/haloperoxidase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14378">
        <property type="entry name" value="PAP2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="SSF48317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106979" description="Uncharacterized protein MJ0662">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="239" mass="27769" checksum="D66F091DB1B0AA61" modified="1996-11-01" version="1">MDKRILVRLAIYPIAYVLWGGLMWYSQIITPLDVTNLLLKLPLCNKEFYIFLQSLPNIVIEFFKIIYLYGFSSMIIGGIAYYLFIKRDFLKSDIILIDLALGWLFAGLIYTFVVVKSPFQVGVAKDLINMHYFWIFTKPTYEIPSLHTAYSFLLALHFKDEKPLNYIYFALAILIPISTLIMGMHWIVDVITGVLYGYIIYKFPKTIHIKISKALDFLAGHIKPCILCGKCKERETHEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q58097</accession>
    <name>MFNC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">(5-formylfuran-3-yl)methyl phosphate transaminase</fullName>
            <ecNumber evidence="3">2.6.1.108</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-HFC-P:alanine aminotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mfnC</name>
        <name type="ordered locus">MJ0684</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="Biochemistry" volume="53" first="4635" last="4647">
            <title>Biosynthesis of the 5-(aminomethyl)-3-furanmethanol moiety of methanofuran.</title>
            <authorList>
                <person name="Miller D."/>
                <person name="Wang Y."/>
                <person name="Xu H."/>
                <person name="Harich K."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="24977328"/>
            <dbReference type="DOI" id="10.1021/bi500615p"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Acta Crystallogr. D" volume="59" first="563" last="565">
            <title>Crystallization and preliminary X-ray analysis of the Mj0684 gene product, a putative aspartate aminotransferase, from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Yang J.K."/>
                <person name="Chang C."/>
                <person name="Cho S.J."/>
                <person name="Lee J.Y."/>
                <person name="Yu Y.G."/>
                <person name="Eom S.H."/>
                <person name="Suh S.W."/>
            </authorList>
            <dbReference type="PubMed" id="12595727"/>
            <dbReference type="DOI" id="10.1107/S0907444903000076"/>
        </citation>
        <scope>CRYSTALLIZATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2008" name="Bull. Korean Chem. Soc." volume="29" first="173" last="176">
            <title>Crystal structure of MJ0684 from Methanococcus jannaschii, a novel archaeal homolog of kynurenine aminotransferase.</title>
            <authorList>
                <person name="Yang J.K."/>
            </authorList>
            <dbReference type="DOI" id="10.5012/bkcs.2008.29.1.173"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH PYRIDOXAL-PHOSPHATE</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transamination reaction between 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) and alanine to produce pyruvate and 5-(aminomethyl)-3-furanmethanol phosphate (F1-P), the precursor for the furan moiety in methanofuran.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + L-alanine = [5-(aminomethyl)-3-furyl]methyl phosphate + pyruvate</text>
            <dbReference type="Rhea" id="RHEA:43564"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:83407"/>
            <dbReference type="ChEBI" id="CHEBI:83431"/>
            <dbReference type="EC" id="2.6.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.108" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870189.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2Z61">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A=1-370"/>
    </dbReference>
    <dbReference type="PDBsum" id="2Z61"/>
    <dbReference type="AlphaFoldDB" id="Q58097"/>
    <dbReference type="SMR" id="Q58097"/>
    <dbReference type="STRING" id="243232.MJ_0684"/>
    <dbReference type="EnsemblBacteria" id="AAB98679">
        <property type="protein sequence ID" value="AAB98679"/>
        <property type="gene ID" value="MJ_0684"/>
    </dbReference>
    <dbReference type="GeneID" id="1451550"/>
    <dbReference type="KEGG" id="mja:MJ_0684"/>
    <dbReference type="eggNOG" id="arCOG01130">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_3_2"/>
    <dbReference type="InParanoid" id="Q58097"/>
    <dbReference type="OMA" id="WIVADEV"/>
    <dbReference type="OrthoDB" id="32104at2157"/>
    <dbReference type="PhylomeDB" id="Q58097"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-18938"/>
    <dbReference type="BRENDA" id="2.6.1.108">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="EvolutionaryTrace" id="Q58097"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123859" description="(5-formylfuran-3-yl)methyl phosphate transaminase">
        <location>
            <begin position="1"/>
            <end position="370"/>
        </location>
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    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="4">
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        </location>
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    </feature>
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        <location>
            <begin position="354"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
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        <source>
            <dbReference type="PubMed" id="12595727"/>
        </source>
    </evidence>
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        <source ref="4"/>
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        <source>
            <dbReference type="PubMed" id="24977328"/>
        </source>
    </evidence>
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    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="2Z61"/>
        </source>
    </evidence>
    <sequence length="370" mass="42348" checksum="51C2EE6D50079940" modified="1998-12-15" version="2">MLSKRLLNFESFEVMDILALAQKLESEGKKVIHLEIGEPDFNTPKPIVDEGIKSLKEGKTHYTDSRGILELREKISELYKDKYKADIIPDNIIITGGSSLGLFFALSSIIDDGDEVLIQNPCYPCYKNFIRFLGAKPVFCDFTVESLEEALSDKTKAIIINSPSNPLGEVIDREIYEFAYENIPYIISDEIYNGLVYEGKCYSAIEFDENLEKTILINGFSKLYAMTGWRIGYVISNDEIIEAILKLQQNLFISAPTISQYAALKAFEKETEREINSMIKEFDRRRRLVLKYVKDFGWEVNNPIGAYYVFPNIGEDGREFAYKLLKEKFVALTPGIGFGSKGKNYIRISYANSYENIKEGLERIKEFLNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58106</accession>
    <name>Y695_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ0695</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0695</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0803.</text>
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        <property type="protein sequence ID" value="AAB98696.1"/>
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    </dbReference>
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    </dbReference>
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    <dbReference type="EnsemblBacteria" id="AAB98696">
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        <property type="gene ID" value="MJ_0695"/>
    </dbReference>
    <dbReference type="GeneID" id="1451562"/>
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    <dbReference type="eggNOG" id="arCOG10950">
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013993" description="Uncharacterized protein MJ0695">
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            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
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    <sequence length="108" mass="13250" checksum="CF0051894326A911" modified="1996-11-01" version="1" precursor="true">MNLWEFRFGKSFLFIPNFIMKVLAFEELALFDIFDSLINVKINIYENGIECGFSFYKWDEFKGYKIEDKYIRLISKFPLIIRLIFVRDIYLRYDEELEGIIEKHLRQK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="130" xmlns="http://uniprot.org/uniprot">
    <accession>Q58108</accession>
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            <fullName evidence="1">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
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        <name evidence="1" type="primary">flpA</name>
        <name type="ordered locus">MJ0697</name>
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="Acta Crystallogr. D" volume="55" first="338" last="340">
            <title>Expression, purification and preliminary X-ray analysis of a fibrillarin homolog from Methanococcus jannaschii, a hyperthermophile.</title>
            <authorList>
                <person name="Wang H."/>
                <person name="Yokota H."/>
                <person name="Kim R."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="10089444"/>
            <dbReference type="DOI" id="10.1107/S0907444998007513"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS)</scope>
        <scope>MASS SPECTROMETRY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="EMBO J." volume="19" first="317" last="323">
            <title>Crystal structure of a fibrillarin homologue from Methanococcus jannaschii, a hyperthermophile, at 1.6-A resolution.</title>
            <authorList>
                <person name="Wang H."/>
                <person name="Boisvert D."/>
                <person name="Kim K.K."/>
                <person name="Kim R."/>
                <person name="Kim S.-H."/>
            </authorList>
            <dbReference type="PubMed" id="10654930"/>
            <dbReference type="DOI" id="10.1093/emboj/19.3.317"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="mass spectrometry" mass="25971.0" method="Electrospray" evidence="2"/>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="A64387"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-230"/>
    </dbReference>
    <dbReference type="PDB" id="1G8S">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-230"/>
    </dbReference>
    <dbReference type="PDBsum" id="1FBN"/>
    <dbReference type="PDBsum" id="1G8S"/>
    <dbReference type="AlphaFoldDB" id="Q58108"/>
    <dbReference type="SMR" id="Q58108"/>
    <dbReference type="IntAct" id="Q58108">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0697"/>
    <dbReference type="EnsemblBacteria" id="AAB98690">
        <property type="protein sequence ID" value="AAB98690"/>
        <property type="gene ID" value="MJ_0697"/>
    </dbReference>
    <dbReference type="GeneID" id="1451564"/>
    <dbReference type="KEGG" id="mja:MJ_0697"/>
    <dbReference type="eggNOG" id="arCOG00078">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059055_2_0_2"/>
    <dbReference type="InParanoid" id="Q58108"/>
    <dbReference type="OMA" id="INMATHR"/>
    <dbReference type="OrthoDB" id="58340at2157"/>
    <dbReference type="PhylomeDB" id="Q58108"/>
    <dbReference type="EvolutionaryTrace" id="Q58108"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D RNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone-glutamine methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990258">
        <property type="term" value="P:histone glutamine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031167">
        <property type="term" value="P:rRNA methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000148533" description="Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="130"/>
            <end position="131"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="150"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="5"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="13"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="19"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
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    </feature>
    <feature type="strand" evidence="3">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="41"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
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            <begin position="47"/>
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        </location>
    </feature>
    <feature type="turn" evidence="3">
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            <begin position="54"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="58"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="77"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
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            <begin position="87"/>
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    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="99"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="108"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="124"/>
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        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="134"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="138"/>
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        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="144"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="180"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="185"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="189"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="205"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="214"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="220"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10089444"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1FBN"/>
        </source>
    </evidence>
    <sequence length="230" mass="25966" checksum="9ECAAD7C4C606756" modified="1996-11-01" version="1">MEDIKIKEIFENIYEVDLGDGLKRIATKSIVKGKKVYDEKIIKIGDEEYRIWNPNKSKLAAAIIKGLKVMPIKRDSKILYLGASAGTTPSHVADIADKGIVYAIEYAPRIMRELLDACAERENIIPILGDANKPQEYANIVEKVDVIYEDVAQPNQAEILIKNAKWFLKKGGYGMIAIKARSIDVTKDPKEIFKEQKEILEAGGFKIVDEVDIEPFEKDHVMFVGIWEGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58128</accession>
    <name>Y718_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter MJ0718</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0718</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chromate ion transporter (CHR) (TC 2.A.51) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64389">
        <property type="entry name" value="F64389"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870223.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58128"/>
    <dbReference type="SMR" id="Q58128"/>
    <dbReference type="STRING" id="243232.MJ_0718"/>
    <dbReference type="DNASU" id="1451595"/>
    <dbReference type="EnsemblBacteria" id="AAB98712">
        <property type="protein sequence ID" value="AAB98712"/>
        <property type="gene ID" value="MJ_0718"/>
    </dbReference>
    <dbReference type="GeneID" id="1451595"/>
    <dbReference type="KEGG" id="mja:MJ_0718"/>
    <dbReference type="eggNOG" id="arCOG06434">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018106_0_0_2"/>
    <dbReference type="InParanoid" id="Q58128"/>
    <dbReference type="OMA" id="YQGAVGH"/>
    <dbReference type="OrthoDB" id="25705at2157"/>
    <dbReference type="PhylomeDB" id="Q58128"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015109">
        <property type="term" value="F:chromate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014047">
        <property type="entry name" value="Chr_Tranpt_l_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003370">
        <property type="entry name" value="Chromate_transpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02417">
        <property type="entry name" value="Chromate_transp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004810">
        <property type="entry name" value="ChrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00937">
        <property type="entry name" value="2A51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000107003" description="Uncharacterized transporter MJ0718">
        <location>
            <begin position="1"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="338"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="402" mass="45132" checksum="A35EBADB62CF9C8B" modified="1996-11-01" version="1">MMMHPMNKTNKRDKMEKRNNPSALNIFMSFLKLGMVAFGGPTAIAYVREMVVDEKKWMDEKSFNNGVALAQIIPGASVMQVAAYVGFYLRGIVGAFAAFMAYALPAFLIMLFLTIIYMHVKSLPKTVSIFEALRIIVVSLAANGTLNFSKKNIRTIGDVFLLLISALLFILKFSPFIVIFVSIFIGFLMYRRDITKLSLKIDIPREKLRIYKYVAYLLFGVFLFNLILYMIDSKLFLLSTLMMKVDVFAFGGGYGSVPFMLHEVVDKYNLMDAKTFMDGIALGQITPGPIVITATFVGYIVGGFIGSIISTISVFTPSFIILLSSIPIFDSLKHNTIFKNILHMILVSFVGLLVAVTIRFALLVDWSIQALIIFIVSFLLLYKKYNMLLVVLLSLVLGYLIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58144</accession>
    <name>RUBY_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative rubrerythrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0734</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May provide oxidative stress protection via catalytic reduction of intracellular hydrogen peroxide.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="1">Binds 3 Fe(3+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Possesses two rubredoxin-like centers and two non-sulfur oxo-bridged di-iron centers per dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AAB98729.1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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        <ligand>
            <name>Fe(3+)</name>
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            <name>Fe(3+)</name>
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            <name>Fe(3+)</name>
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            <name>Fe(3+)</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P24931"/>
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    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00085"/>
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    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00241"/>
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    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="204" mass="23835" checksum="25B2ABFB53DB6769" modified="1996-11-01" version="1">MINNFFVINMKETLKNLTKAYIGESLARNRYTCYAKIAKQEGYEQIAEIFLLTAENEREHAKWLYYLITELKKKYNIDDKAIKVDGVEVPIVLGNTAENLKASIEGEHFEHTEMYPKFADIAEKEGLKEIADRLRAIGIAEKHHEERFKKLLKEVEEGTVFKKDKPVEWVCRKCGFVHLGKEPPEKCPSCSHPRKYFEVKCEKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q58206</accession>
    <name>Y796_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ABC transporter ATP-binding protein MJ0796</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0796</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="J. Biol. Chem." volume="276" first="32313" last="32321">
            <title>The crystal structure of the MJ0796 ATP-binding cassette. Implications for the structural consequences of ATP hydrolysis in the active site of an ABC transporter.</title>
            <authorList>
                <person name="Yuan Y.-R."/>
                <person name="Blecker S."/>
                <person name="Martsinkevich O."/>
                <person name="Millen L."/>
                <person name="Thomas P.J."/>
                <person name="Hunt J.F."/>
            </authorList>
            <dbReference type="PubMed" id="11402022"/>
            <dbReference type="DOI" id="10.1074/jbc.M100758200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS)</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2002" name="Mol. Cell" volume="10" first="139" last="149">
            <title>ATP binding to the motor domain from an ABC transporter drives formation of a nucleotide sandwich dimer.</title>
            <authorList>
                <person name="Smith P.C."/>
                <person name="Karpowich N."/>
                <person name="Millen L."/>
                <person name="Moody J.E."/>
                <person name="Rosen J."/>
                <person name="Thomas P.J."/>
                <person name="Hunt J.F."/>
            </authorList>
            <dbReference type="PubMed" id="12150914"/>
            <dbReference type="DOI" id="10.1016/S1097-2765(02)00576-2"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF MUTANT GLN-171</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D64399"/>
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    <dbReference type="RefSeq" id="WP_010870305.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="PDB" id="1F3O">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A=1-235"/>
    </dbReference>
    <dbReference type="PDB" id="1L2T">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B=1-235"/>
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    <dbReference type="PDB" id="3TIF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.80 A"/>
        <property type="chains" value="A/B=1-235"/>
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        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="ABC_MJ0796_LolCDE_FtsE"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
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    <dbReference type="InterPro" id="IPR017911">
        <property type="entry name" value="MacB_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SSF52540"/>
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    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
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        <location>
            <begin position="1"/>
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            <begin position="2"/>
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        </location>
    </feature>
    <feature type="binding site" evidence="1">
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            <begin position="38"/>
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        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <begin position="2"/>
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            <begin position="16"/>
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        </source>
    </evidence>
    <sequence length="235" mass="26566" checksum="C3035AE896099E72" modified="1996-11-01" version="1">MIKLKNVTKTYKMGEEIIYALKNVNLNIKEGEFVSIMGPSGSGKSTMLNIIGCLDKPTEGEVYIDNIKTNDLDDDELTKIRRDKIGFVFQQFNLIPLLTALENVELPLIFKYRGAMSGEERRKRALECLKMAELEERFANHKPNQLSGGQQQRVAIARALANNPPIILADEPTGALDSKTGEKIMQLLKKLNEEDGKTVVVVTHDINVARFGERIIYLKDGEVEREEKLRGFDDR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58219</accession>
    <name>Y809_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Acylphosphatase-like protein MJ0809</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0809</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64401">
        <property type="entry name" value="A64401"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58219"/>
    <dbReference type="SMR" id="Q58219"/>
    <dbReference type="EnsemblBacteria" id="AAB98817">
        <property type="protein sequence ID" value="AAB98817"/>
        <property type="gene ID" value="MJ_0809"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0809"/>
    <dbReference type="eggNOG" id="arCOG01674">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1656874_0_0_2"/>
    <dbReference type="InParanoid" id="Q58219"/>
    <dbReference type="OMA" id="ETREEPY"/>
    <dbReference type="PhylomeDB" id="Q58219"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020456">
        <property type="entry name" value="Acylphosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47268">
        <property type="entry name" value="PTHR47268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="SSF54975"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158563" description="Acylphosphatase-like protein MJ0809">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Acylphosphatase-like" evidence="1">
        <location>
            <begin position="8"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <sequence length="167" mass="19723" checksum="DD998FE1329C0042" modified="1996-11-01" version="1">MVIIMPTTYELKIYGKVQHVGFRDRIENIGKGLGINGIIYNYKDGTVRILANFDDEEDKELFKKYIERLEKKDKLIEIEKIEEKRLNTYIEFPEGISRLSSDDILELNKKLDEGVKYIKLIFAELEEHKKVLLEIKDMHVKTVEILSEHTRILNEIKDTLKDIKDKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58228</accession>
    <name>Y818_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0818</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0818</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64402">
        <property type="entry name" value="B64402"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870329.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58228"/>
    <dbReference type="SMR" id="Q58228"/>
    <dbReference type="STRING" id="243232.MJ_0818"/>
    <dbReference type="EnsemblBacteria" id="AAB98819">
        <property type="protein sequence ID" value="AAB98819"/>
        <property type="gene ID" value="MJ_0818"/>
    </dbReference>
    <dbReference type="GeneID" id="1451701"/>
    <dbReference type="KEGG" id="mja:MJ_0818"/>
    <dbReference type="eggNOG" id="arCOG05052">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116581_0_0_2"/>
    <dbReference type="OMA" id="KRDKYRH"/>
    <dbReference type="OrthoDB" id="68219at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035451">
        <property type="entry name" value="Ada-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016798">
        <property type="entry name" value="UCP021980_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021980">
        <property type="entry name" value="UCP021980_RNA-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57884">
        <property type="entry name" value="SSF57884"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107062" description="Uncharacterized protein MJ0818">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <sequence length="203" mass="23754" checksum="4715370D01BB0A9E" modified="1996-11-01" version="1">MKLTEKNITLFALTCFVIISTTWLFLNPIQPKEKHIAEIKEGDYVVIKGYIQEMYVKRDKYRHVINISRIVINDGTGNLDVVAFGKTREELLTYILSYNPMIKEGDYIEVKGRVTLYKGKYEIILNNIKDFKLLKKNNFERDIYLSPTPTGIYASKYGKKYHTSKNCPYGKRLKEENIIYFYSEDDAKALGYEKCKWCEEHGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="60" xmlns="http://uniprot.org/uniprot">
    <accession>Q58238</accession>
    <name>Y828_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0828</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0828</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64403">
        <property type="entry name" value="D64403"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58238"/>
    <dbReference type="SMR" id="Q58238"/>
    <dbReference type="STRING" id="243232.MJ_0828"/>
    <dbReference type="EnsemblBacteria" id="AAB98827">
        <property type="protein sequence ID" value="AAB98827"/>
        <property type="gene ID" value="MJ_0828"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0828"/>
    <dbReference type="eggNOG" id="arCOG01915">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2820876_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107065" description="Uncharacterized protein MJ0828">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <sequence length="66" mass="7485" checksum="C1C9DF3595FFA7CD" modified="1996-11-01" version="1">MRIEAEGQAKAIQIVAEAARQYFKDEAQLYKALEVANNVLKDNAKYVISENILDVVRNFIKKDKSA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58280</accession>
    <name>FSR_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Coenzyme F420-dependent sulfite reductase</fullName>
            <ecNumber evidence="3">1.8.98.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Sulfite reductase (coenzyme F420)</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">fsr</name>
        <name type="ordered locus">MJ0870</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Biol. Chem." volume="280" first="38776" last="38786">
            <title>A new type of sulfite reductase, a novel coenzyme F420-dependent enzyme, from the methanarchaeon Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Johnson E.F."/>
                <person name="Mukhopadhyay B."/>
            </authorList>
            <dbReference type="PubMed" id="16048999"/>
            <dbReference type="DOI" id="10.1074/jbc.M503492200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>INDUCTION</scope>
        <scope>DOMAIN</scope>
        <scope>IDENTIFICATION BY MASS SPECTROMETRY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="Appl. Environ. Microbiol." volume="74" first="3591" last="3595">
            <title>Coenzyme F420-dependent sulfite reductase-enabled sulfite detoxification and use of sulfite as a sole sulfur source by Methanococcus maripaludis.</title>
            <authorList>
                <person name="Johnson E.F."/>
                <person name="Mukhopadhyay B."/>
            </authorList>
            <dbReference type="PubMed" id="18378657"/>
            <dbReference type="DOI" id="10.1128/AEM.00098-08"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>EXPRESSION IN M.MARIPALUDIS</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the reduction of sulfite to sulfide using reduced F420 as the electron source. Involved in sulfite detoxification and assimilation. Cannot use NADH or NADPH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>2 H(+) + 3 H2O + hydrogen sulfide + 3 oxidized coenzyme F420-(gamma-L-Glu)(n) = 3 reduced coenzyme F420-(gamma-L-Glu)(n) + sulfite</text>
            <dbReference type="Rhea" id="RHEA:42808"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17359"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.8.98.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 4 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </cofactor>
        <text evidence="1">Binds 1 siroheme per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">12.2 uM for sulfite</KM>
            <KM evidence="3">21.2 uM for reduced coenzyme F420</KM>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 7.0.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is above 95 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="induction">
        <text evidence="3">Induced by sulfite.</text>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains an N-terminal H(2)F420 dehydrogenase domain and a C-terminal dissimilatory-type siroheme sulfite reductase domain.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">Expression in Methanococcus maripaludis, a sulfite-sensitive methanogen, leads to sulfite detoxification and use of sulfite as a sole sulfur source by M. maripaludis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.</text>
    </comment>
    <dbReference type="EC" id="1.8.98.3" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64408">
        <property type="entry name" value="F64408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870385.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="7NP8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=1-620"/>
    </dbReference>
    <dbReference type="PDBsum" id="7NP8"/>
    <dbReference type="AlphaFoldDB" id="Q58280"/>
    <dbReference type="SMR" id="Q58280"/>
    <dbReference type="STRING" id="243232.MJ_0870"/>
    <dbReference type="PRIDE" id="Q58280"/>
    <dbReference type="EnsemblBacteria" id="AAB98876">
        <property type="protein sequence ID" value="AAB98876"/>
        <property type="gene ID" value="MJ_0870"/>
    </dbReference>
    <dbReference type="GeneID" id="1451759"/>
    <dbReference type="KEGG" id="mja:MJ_0870"/>
    <dbReference type="eggNOG" id="arCOG02061">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_428041_0_0_2"/>
    <dbReference type="InParanoid" id="Q58280"/>
    <dbReference type="OMA" id="GDECWKP"/>
    <dbReference type="OrthoDB" id="49654at2157"/>
    <dbReference type="BRENDA" id="1.8.98.3">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.413.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005117">
        <property type="entry name" value="NiRdtase/SiRdtase_haem-b_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036136">
        <property type="entry name" value="Nit/Sulf_reduc_fer-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006067">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045854">
        <property type="entry name" value="NO2/SO3_Rdtase_4Fe4S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006066">
        <property type="entry name" value="NO2/SO3_Rdtase_FeS/sirohaem_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="PTHR31332"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01077">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03460">
        <property type="entry name" value="NIR_SIR_ferr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00397">
        <property type="entry name" value="SIROHAEM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55124">
        <property type="entry name" value="SSF55124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56014">
        <property type="entry name" value="SSF56014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00365">
        <property type="entry name" value="NIR_SIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0349">Heme</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000199970" description="Coenzyme F420-dependent sulfite reductase">
        <location>
            <begin position="1"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="486"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 3" evidence="2">
        <location>
            <begin position="520"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="434"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="1">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>siroheme</name>
            <dbReference type="ChEBI" id="CHEBI:60052"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="495"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="498"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="524"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="527"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="534"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q59109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16048999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="18378657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="16048999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="620" mass="69794" checksum="9D71D2580D7D0BA8" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="68" xmlns="http://uniprot.org/uniprot">
    <accession>Q58284</accession>
    <name>Y874_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0874</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0874</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To B.subtilis YdcN C-terminal region.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64409">
        <property type="entry name" value="B64409"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58284"/>
    <dbReference type="STRING" id="243232.MJ_0874"/>
    <dbReference type="DNASU" id="1451763"/>
    <dbReference type="EnsemblBacteria" id="AAB98881">
        <property type="protein sequence ID" value="AAB98881"/>
        <property type="gene ID" value="MJ_0874"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0874"/>
    <dbReference type="eggNOG" id="arCOG00199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2392908_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107087" description="Uncharacterized protein MJ0874">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="93" mass="10860" checksum="07603737C0CC494D" modified="1996-11-01" version="1">MMLLLAILHKNDADYFIAEAMGVKIIEEEAYKKISKETFEEALKELKNSDVVVYTDFPIGEMNELNLKLIEEAKNLKKRMIFYEDDISVLKEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58291</accession>
    <name>OTC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Ornithine carbamoyltransferase</fullName>
            <shortName>OTCase</shortName>
            <ecNumber>2.1.3.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">argF</name>
        <name type="ordered locus">MJ0881</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>carbamoyl phosphate + L-ornithine = H(+) + L-citrulline + phosphate</text>
            <dbReference type="Rhea" id="RHEA:19513"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. OTCase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64410">
        <property type="entry name" value="A64410"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870395.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58291"/>
    <dbReference type="SMR" id="Q58291"/>
    <dbReference type="STRING" id="243232.MJ_0881"/>
    <dbReference type="EnsemblBacteria" id="AAB98885">
        <property type="protein sequence ID" value="AAB98885"/>
        <property type="gene ID" value="MJ_0881"/>
    </dbReference>
    <dbReference type="GeneID" id="1451770"/>
    <dbReference type="KEGG" id="mja:MJ_0881"/>
    <dbReference type="eggNOG" id="arCOG00912">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043846_3_2_2"/>
    <dbReference type="InParanoid" id="Q58291"/>
    <dbReference type="OMA" id="DGNNVCN"/>
    <dbReference type="OrthoDB" id="43727at2157"/>
    <dbReference type="PhylomeDB" id="Q58291"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00112"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004585">
        <property type="term" value="F:ornithine carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019240">
        <property type="term" value="P:citrulline biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01109">
        <property type="entry name" value="OTCase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002292">
        <property type="entry name" value="Orn/put_carbamltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024904">
        <property type="entry name" value="OTCase_ArgI"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00102">
        <property type="entry name" value="OTCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="SSF53671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00658">
        <property type="entry name" value="orni_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000113065" description="Ornithine carbamoyltransferase">
        <location>
            <begin position="1"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="226"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="262"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="305" mass="34569" checksum="98F12CBA15BB319A" modified="1996-11-01" version="1">MHLLDLDVLSREDVLKIIEYGIYFKKNRRKHEKILEGKSVAILFEKPSTRTRMSFDIAVYELGGHPLIMNQNEIHLGKKESIKDTAKVMGRYVDTIVARVYKHRHLEEMAKYSSVPVINALSDLAHPCQILADLMTIKEYKGKFKGLKIAYLGDGNNVCNSLILGSALVGMDTYVGTPKGYEPNAKVVLKAKEIINNYGEGSLTLTNDPIEAAEDADVLYTDVWISMGDDKDKEEVLKIFPPFQINSKLLEYAKDDVIVMHCLPANRGYEITDDVIDGEHSVVYDEAENRLHVQKGVFKFIFERK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58301</accession>
    <name>FLAB1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Flagellin B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaB1</name>
        <name type="ordered locus">MJ0891</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal flagellin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64411">
        <property type="entry name" value="C64411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870405.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="5YA6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.50 A"/>
        <property type="chains" value="A/B=13-217"/>
    </dbReference>
    <dbReference type="PDBsum" id="5YA6"/>
    <dbReference type="AlphaFoldDB" id="Q58301"/>
    <dbReference type="SMR" id="Q58301"/>
    <dbReference type="STRING" id="243232.MJ_0891"/>
    <dbReference type="EnsemblBacteria" id="AAB98894">
        <property type="protein sequence ID" value="AAB98894"/>
        <property type="gene ID" value="MJ_0891"/>
    </dbReference>
    <dbReference type="GeneID" id="1451780"/>
    <dbReference type="KEGG" id="mja:MJ_0891"/>
    <dbReference type="eggNOG" id="arCOG01829">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051124_0_1_2"/>
    <dbReference type="InParanoid" id="Q58301"/>
    <dbReference type="OMA" id="GESHVLN"/>
    <dbReference type="OrthoDB" id="94462at2157"/>
    <dbReference type="PhylomeDB" id="Q58301"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="PTHR35903"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Arch_flagellin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="propeptide" id="PRO_0000009373" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000009374" description="Flagellin B1">
        <location>
            <begin position="13"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="73"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="77"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="100"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="130"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="134"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="155"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="166"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="173"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="186"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="194"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="199"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="211"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5YA6"/>
        </source>
    </evidence>
    <sequence length="217" mass="22700" checksum="4374437380061565" modified="1997-11-01" version="1" precursor="true">MKVFEFLKGKRGAMGIGTLIIFIAMVLVAAVAAAVLINTSGFLQQKAMATGKESTEQVASGLMCIGVTGHYDKTLGGIDKLAIYITPNAGSAPIDLKNAKLFLIYDGESHVLNYSTVTTATLGADDIFNSSAITDWSLADSSSYVVGVIQDADGSLSNGVINKGDIAVLLVNANAVFNKAIPTRSEVSGQFQPEFGAPAVIQFTTPAAYTQTVIELQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q58314</accession>
    <name>Y904_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0904</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0904</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64412">
        <property type="entry name" value="H64412"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870418.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58314"/>
    <dbReference type="STRING" id="243232.MJ_0904"/>
    <dbReference type="EnsemblBacteria" id="AAB98914">
        <property type="protein sequence ID" value="AAB98914"/>
        <property type="gene ID" value="MJ_0904"/>
    </dbReference>
    <dbReference type="GeneID" id="1451793"/>
    <dbReference type="KEGG" id="mja:MJ_0904"/>
    <dbReference type="eggNOG" id="arCOG05057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1393583_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107096" description="Uncharacterized protein MJ0904">
        <location>
            <begin position="1"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="195" mass="21909" checksum="ED92E6C6B7E30392" modified="1996-11-01" version="1">MNIENLAYNSSSDVLKAKIKNDKFPVNLTVQYWVDVSRGSNIYYKSSIFQTEIYPKSEKELIVPLTLGDLESGIYNITLYVRVNNFALFNYQKVPVILKKSISIEINGSKGVMQQKSNKESDEIINETSETHKNMTIDIKNLSNNKDNKSNIEESTAKNVKSNIETKKSADNNSILGKISGFFGSIVSTIFSLFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58330</accession>
    <name>Y920_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0920</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0920</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64414">
        <property type="entry name" value="H64414"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870434.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58330"/>
    <dbReference type="SMR" id="Q58330"/>
    <dbReference type="STRING" id="243232.MJ_0920"/>
    <dbReference type="PRIDE" id="Q58330"/>
    <dbReference type="EnsemblBacteria" id="AAB98924">
        <property type="protein sequence ID" value="AAB98924"/>
        <property type="gene ID" value="MJ_0920"/>
    </dbReference>
    <dbReference type="GeneID" id="1451809"/>
    <dbReference type="KEGG" id="mja:MJ_0920"/>
    <dbReference type="eggNOG" id="arCOG00354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1472124_0_0_2"/>
    <dbReference type="InParanoid" id="Q58330"/>
    <dbReference type="OMA" id="VGGTTKM"/>
    <dbReference type="OrthoDB" id="79086at2157"/>
    <dbReference type="PhylomeDB" id="Q58330"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107105" description="Uncharacterized protein MJ0920">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="G">
        <location>
            <begin position="17"/>
            <end position="137"/>
        </location>
    </feature>
    <sequence length="185" mass="21241" checksum="AD53E72ED4DB1FAE" modified="1996-11-01" version="1">MQDKEFKIAIIGPENAGKSSIMNALFGKYVSLVSEVGGTTKMPIKRYWGKLKIGRIKEEPEFVNLVFVDLGGLYTTTDKQSPIMTPKVLEKTFEEINDSDMIIHVIDGSVGLLRSFERLHHLLKFRYQKPIIVVINKCDLLNDSDKEHLKNYVERRIKNTPIFVSAKTFEGIPELLDIIIKYLKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58334</accession>
    <name>Y924_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0924</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0924</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0547 and MJ0169.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64415">
        <property type="entry name" value="D64415"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58334"/>
    <dbReference type="SMR" id="Q58334"/>
    <dbReference type="STRING" id="243232.MJ_0924"/>
    <dbReference type="EnsemblBacteria" id="AAB98928">
        <property type="protein sequence ID" value="AAB98928"/>
        <property type="gene ID" value="MJ_0924"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0924"/>
    <dbReference type="eggNOG" id="arCOG00589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1154355_0_0_2"/>
    <dbReference type="OMA" id="QSYAKKE"/>
    <dbReference type="PhylomeDB" id="Q58334"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107109" description="Uncharacterized ATP-binding protein MJ0924">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="16"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="263" mass="29130" checksum="EF2020594D327CA0" modified="1996-11-01" version="1">MDYRWIQMKVITFSIAKGGTGKTIITANAAAALAKKGKKILLIDGDVGSKSLSHLLNVKSNIFLADIIEEERPIKDAIVNTPIENIELLAVGKSLADYLKFDINILKRFKELGDYDYVFIDAPSTSSGVETYLALGLSDYFIPVLDYTAFGPSLQGAINTIVIGKNYLESTPAGFIINKAEDLPESVINDIKKILGLECISIIHKNSLVEQSYAKKEIVYLTSSDKKFVEEIDKIVDALEKLKEVKERDIPKVIEKIKESTLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58347</accession>
    <name>PUR2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="2">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">purD</name>
        <name type="ordered locus">MJ0937</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-phospho-beta-D-ribosylamine + ATP + glycine = ADP + H(+) + N(1)-(5-phospho-beta-D-ribosyl)glycinamide + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GARS family.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64417">
        <property type="entry name" value="A64417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870451.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58347"/>
    <dbReference type="SMR" id="Q58347"/>
    <dbReference type="STRING" id="243232.MJ_0937"/>
    <dbReference type="EnsemblBacteria" id="AAB98942">
        <property type="protein sequence ID" value="AAB98942"/>
        <property type="gene ID" value="MJ_0937"/>
    </dbReference>
    <dbReference type="GeneID" id="1451833"/>
    <dbReference type="KEGG" id="mja:MJ_0937"/>
    <dbReference type="eggNOG" id="arCOG04415">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027420_3_0_2"/>
    <dbReference type="InParanoid" id="Q58347"/>
    <dbReference type="OMA" id="KATVCKY"/>
    <dbReference type="OrthoDB" id="58022at2157"/>
    <dbReference type="PhylomeDB" id="Q58347"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PTHR43472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="SSF51246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151510" description="Phosphoribosylamine--glycine ligase">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="109"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="140"/>
            <end position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="294"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <sequence length="444" mass="49098" checksum="9AD115FBC8AB8BD1" modified="1996-11-01" version="1">MKILLIGGGARESAIAHALKKNEEVKLYTLMKNKNPGIARLSEEIKLAKETDLDAVKEFAEKVKPDLAVIGPEAPLGEGVVDLLEEMGISAVGPKKLAAQIETNKEFMRNLFKKYNIKGSLMYKAFEEYGEELESFIDELTEKGIKAVVKPVGLTGGKGVKVVGEQLKDNEEAKKYAKEIFETGLGGGKVLIEEKLEGVEFTLHGFVDGDTIKFTPFVQDHPHALEGDEGSITGGMGSYSCPDHKLPFMTEEDVKLAKEIMEETVKALKEEVGGYKGILYGQFMLTKEGPKIIEYNARFGDPEAMNLLAILKNDFLEVCEAIVNKKLKDIDVEFENKATVCKYVVPKGYPDNPVRGEPITVDEEAIKKTGAILHYASVNEDNGSLYMTGSRAVAVVGVADTIEEAERIAEEATKYIKGEVYHRSDIGKKELIKKRIEKMNELRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58348</accession>
    <name>Y938_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0014 membrane protein MJ0938</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0938</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0014 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64417">
        <property type="entry name" value="B64417"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58348"/>
    <dbReference type="STRING" id="243232.MJ_0938"/>
    <dbReference type="EnsemblBacteria" id="AAB98943">
        <property type="protein sequence ID" value="AAB98943"/>
        <property type="gene ID" value="MJ_0938"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0938"/>
    <dbReference type="eggNOG" id="arCOG04953">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076147_1_0_2"/>
    <dbReference type="InParanoid" id="Q58348"/>
    <dbReference type="OMA" id="PWYEPQY"/>
    <dbReference type="PhylomeDB" id="Q58348"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005226">
        <property type="entry name" value="UPF0014_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30028">
        <property type="entry name" value="PTHR30028"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03649">
        <property type="entry name" value="UPF0014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00245">
        <property type="entry name" value="TIGR00245"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000217860" description="UPF0014 membrane protein MJ0938">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="250" mass="27984" checksum="320012908D9C7F22" modified="1996-11-01" version="1">MIIKVMVMLLIELTYAFIFVIIAVLIAYREKLGIEKKILYVSILALIQLFILGFVLLYIFSFGMVGAFLMIGVMITLASYLIMREINLKNKTKLFICLFITFLTTTIVSLAVLTIPKVVKFEPIYVIPLMGMVIGNTMNTIHLALDKIIDMVKSERDILWGYLALGATEIEALRPFIKNAVKSAVIPQMNRTKSVGVIFIPGAMVGMLLSGANPIYAAEIQIIIMWMILSSAVISGILICYLMYKEIIRA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58352</accession>
    <name>Y942_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable ATP-dependent helicase MJ0942</fullName>
            <ecNumber>3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0942</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the helicase family. DinG subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64417">
        <property type="entry name" value="F64417"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870456.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58352"/>
    <dbReference type="SMR" id="Q58352"/>
    <dbReference type="STRING" id="243232.MJ_0942"/>
    <dbReference type="PRIDE" id="Q58352"/>
    <dbReference type="EnsemblBacteria" id="AAB98945">
        <property type="protein sequence ID" value="AAB98945"/>
        <property type="gene ID" value="MJ_0942"/>
    </dbReference>
    <dbReference type="GeneID" id="1451838"/>
    <dbReference type="KEGG" id="mja:MJ_0942"/>
    <dbReference type="eggNOG" id="arCOG00770">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012117_2_0_2"/>
    <dbReference type="InParanoid" id="Q58352"/>
    <dbReference type="OMA" id="QVNPVNE"/>
    <dbReference type="OrthoDB" id="31834at2157"/>
    <dbReference type="PhylomeDB" id="Q58352"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006139">
        <property type="term" value="P:nucleobase-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="PTHR11472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000102009" description="Probable ATP-dependent helicase MJ0942">
        <location>
            <begin position="1"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="6"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="449"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box">
        <location>
            <begin position="195"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="41"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="651" mass="75957" checksum="1E55B13938D315B2" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58362</accession>
    <name>PFDA1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Prefoldin subunit alpha 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GimC subunit alpha 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pfdA1</name>
        <name type="synonym">pfdA</name>
        <name type="ordered locus">MJ0952</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64418">
        <property type="entry name" value="H64418"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58362"/>
    <dbReference type="SMR" id="Q58362"/>
    <dbReference type="STRING" id="243232.MJ_0952"/>
    <dbReference type="PRIDE" id="Q58362"/>
    <dbReference type="EnsemblBacteria" id="AAB98957">
        <property type="protein sequence ID" value="AAB98957"/>
        <property type="gene ID" value="MJ_0952"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0952"/>
    <dbReference type="eggNOG" id="arCOG01341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091867_1_2_2"/>
    <dbReference type="InParanoid" id="Q58362"/>
    <dbReference type="OMA" id="ELQNQFM"/>
    <dbReference type="PhylomeDB" id="Q58362"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PTHR12674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00293">
        <property type="entry name" value="TIGR00293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153674" description="Prefoldin subunit alpha 1">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="142" mass="16080" checksum="770B816F0A98517A" modified="1996-11-01" version="1">MENMAEDLRQKAMALEIYNQQLQMIQSEITSIRALKSEIMNSIKTIENIKADEETLIPVGPGVFLKAKIVDDKALIGVKSDIYVEKSFNEVIEDLKKSVEDLDKAEKEGMKKAEELAKAITALRKELQTEIQKAQQAQDKKQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58372</accession>
    <name>RNP4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 4</fullName>
            <shortName evidence="1">RNase P component 4</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp4</name>
        <name type="ordered locus">MJ0962</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Nucleic Acids Res." volume="36" first="4172" last="4180">
            <title>Studies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysis.</title>
            <authorList>
                <person name="Pulukkunat D.K."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="18558617"/>
            <dbReference type="DOI" id="10.1093/nar/gkn360"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP1</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="411" first="368" last="383">
            <title>Cooperative RNP assembly: complementary rescue of structural defects by protein and RNA subunits of archaeal RNase P.</title>
            <authorList>
                <person name="Chen W.Y."/>
                <person name="Xu Y."/>
                <person name="Cho I.M."/>
                <person name="Oruganti S.V."/>
                <person name="Foster M.P."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="21683084"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2011.05.012"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP1</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2012" name="Nucleic Acids Res." volume="40" first="4666" last="4680">
            <title>Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors.</title>
            <authorList>
                <person name="Chen W.Y."/>
                <person name="Singh D."/>
                <person name="Lai L.B."/>
                <person name="Stiffler M.A."/>
                <person name="Lai H.D."/>
                <person name="Foster M.P."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="22298511"/>
            <dbReference type="DOI" id="10.1093/nar/gks013"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP1</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 4">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4">Consists of a catalytic RNA component and at least 4 protein subunits. Forms a subcomplex with Rnp1 which stimulates the catalytic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64420">
        <property type="entry name" value="B64420"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870476.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6K0A">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.60 A"/>
        <property type="chains" value="G/H=1-128"/>
    </dbReference>
    <dbReference type="PDB" id="6K0B">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.30 A"/>
        <property type="chains" value="G/H=1-128"/>
    </dbReference>
    <dbReference type="PDBsum" id="6K0A"/>
    <dbReference type="PDBsum" id="6K0B"/>
    <dbReference type="AlphaFoldDB" id="Q58372"/>
    <dbReference type="SMR" id="Q58372"/>
    <dbReference type="STRING" id="243232.MJ_0962"/>
    <dbReference type="EnsemblBacteria" id="AAB98964">
        <property type="protein sequence ID" value="AAB98964"/>
        <property type="gene ID" value="MJ_0962"/>
    </dbReference>
    <dbReference type="GeneID" id="1451860"/>
    <dbReference type="KEGG" id="mja:MJ_0962"/>
    <dbReference type="eggNOG" id="arCOG04345">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079140_3_1_2"/>
    <dbReference type="InParanoid" id="Q58372"/>
    <dbReference type="OMA" id="HVVITCL"/>
    <dbReference type="OrthoDB" id="107253at2157"/>
    <dbReference type="PhylomeDB" id="Q58372"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000153852" description="Ribonuclease P protein component 4">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18558617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="21683084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22298511"/>
        </source>
    </evidence>
    <sequence length="128" mass="15567" checksum="462D677CE5B159D3" modified="1996-11-01" version="1">MKKFLEKKLKKIAYERIDILMSLAEEEAKKGNWDRAKRYVYLARRIAMKMRIRFPKKWKRRICKKCGTFLLYGRNARVRIKSKRYPHVVITCLECGAIYRIPMIREKKEKRRKKLEERLKAKSNSQTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58376</accession>
    <name>Y966_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0966</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0966</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64420">
        <property type="entry name" value="F64420"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58376"/>
    <dbReference type="SMR" id="Q58376"/>
    <dbReference type="STRING" id="243232.MJ_0966"/>
    <dbReference type="EnsemblBacteria" id="AAB98968">
        <property type="protein sequence ID" value="AAB98968"/>
        <property type="gene ID" value="MJ_0966"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0966"/>
    <dbReference type="eggNOG" id="arCOG01355">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011543_3_3_2"/>
    <dbReference type="InParanoid" id="Q58376"/>
    <dbReference type="OMA" id="PESYNWS"/>
    <dbReference type="PhylomeDB" id="Q58376"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000107122" description="Uncharacterized protein MJ0966">
        <location>
            <begin position="1"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="164"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="444" mass="51348" checksum="25ABAB5890A20C70" modified="1996-11-01" version="1">MIKMIKNVAIIYPNKFKAGISCLAVHVLANHLSKYRDLNVGVYFLENYDRIKNFDAIFITLQYENDYFNAIKIIKDLRKNNPNAIFVAGGPCVMENFFPIAEFFDVFIVGEIEGSDVMLKVINREFDVEGVYSKYLEKDKVKRIYPKKLTIDDYPIYQPTSEEGAYGKSFLLEIGRGCPRRCRFCLARAIYYPPRFRKLDDLMYLAEEGVKVNKVNKVALIAPSVGDYKYIVELCNFLDDMGVHISPSSLRADTLNDDLMRILKPKTLTIAPEAGSERLREFIKKDIRERDIANAIDLAKKFGVEKVKLYFMVGIPTETDEDIEELINLTKKVKKEIRKVEISVNPMIPKPHTDFEVEEFDLSSKKKIKYIEKALKKEGIRVEYENFNSMICQCILARGDENLSKYLDYSKNPTSLISALKKDRLLDKYLGRFEDKGVWKNIIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="64" xmlns="http://uniprot.org/uniprot">
    <accession>Q58393</accession>
    <name>Y986_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0986</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0986</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64423">
        <property type="entry name" value="B64423"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58393"/>
    <dbReference type="SMR" id="Q58393"/>
    <dbReference type="EnsemblBacteria" id="AAB98995">
        <property type="protein sequence ID" value="AAB98995"/>
        <property type="gene ID" value="MJ_0986"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0986"/>
    <dbReference type="eggNOG" id="arCOG07514">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202286_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107130" description="Uncharacterized protein MJ0986">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <sequence length="75" mass="8731" checksum="2C32908691499D11" modified="1998-01-01" version="2">MCLICVLGEYIMTQREKDNNIKRVQVTFTKSQWELIENFRGILGQTDAEIVRTIVLTWLSEKSIITTIKKEIGDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58400</accession>
    <name>Y993_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative hydrogenase expression/formation protein MJ0993</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0993</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the HypD family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64424">
        <property type="entry name" value="A64424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870507.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58400"/>
    <dbReference type="SMR" id="Q58400"/>
    <dbReference type="STRING" id="243232.MJ_0993"/>
    <dbReference type="EnsemblBacteria" id="AAB98998">
        <property type="protein sequence ID" value="AAB98998"/>
        <property type="gene ID" value="MJ_0993"/>
    </dbReference>
    <dbReference type="GeneID" id="1451891"/>
    <dbReference type="KEGG" id="mja:MJ_0993"/>
    <dbReference type="eggNOG" id="arCOG04428">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048562_1_0_2"/>
    <dbReference type="InParanoid" id="Q58400"/>
    <dbReference type="OMA" id="HVCGSHE"/>
    <dbReference type="OrthoDB" id="24633at2157"/>
    <dbReference type="PhylomeDB" id="Q58400"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070025">
        <property type="term" value="F:carbon monoxide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11750">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002780">
        <property type="entry name" value="Hyd_form_HypD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042243">
        <property type="entry name" value="HypD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042244">
        <property type="entry name" value="HypD_2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149">
        <property type="entry name" value="PTHR30149"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01924">
        <property type="entry name" value="HypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005622">
        <property type="entry name" value="Hydrgn_mat_hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00075">
        <property type="entry name" value="hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000201454" description="Putative hydrogenase expression/formation protein MJ0993">
        <location>
            <begin position="1"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P24192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="358" mass="40142" checksum="8BE027849D879A77" modified="1996-11-01" version="1">MININDRALIKKAIDKINKLAEKIDKLKIMHVCGSHEHTICKYGIRDVLPENITVVPGPGCPVCVTTQKEIDTAIYLADNGYVITTLGDMYRVPGSEKSLMEKQSEGCDVRIVYSISEAVKMAKKERDKKFVFVAIGFETTAPTTGAELISLKNKDVNNFFILNCHRQTPPVMEFLLNEGVYLDAFICPGHVSTITGLKPYYGLCEKYKAPMVVAGFEPIDVLMAIIMILKQVISGEAKVENEYIRAVKPEGNVLAQKIINEVFESIDVPWRGFPVVKNGGFGLREKYKKFDIYEHEDIPEIKEKIPKGCICDKILRGEKLPTDCPLFGTVCTPLNPVGSCMVSDEGTCRIFYKYRRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58412</accession>
    <name>Y1006_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ1006</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1006</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64425">
        <property type="entry name" value="E64425"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58412"/>
    <dbReference type="STRING" id="243232.MJ_1006"/>
    <dbReference type="PRIDE" id="Q58412"/>
    <dbReference type="DNASU" id="1451903"/>
    <dbReference type="EnsemblBacteria" id="AAB99014">
        <property type="protein sequence ID" value="AAB99014"/>
        <property type="gene ID" value="MJ_1006"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1006"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q58412"/>
    <dbReference type="OMA" id="WKFGVEE"/>
    <dbReference type="PhylomeDB" id="Q58412"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184672" description="Uncharacterized ATP-binding protein MJ1006">
        <location>
            <begin position="1"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="359" mass="42777" checksum="7D2F72172C6E8BC4" modified="1997-11-01" version="1">MAIQNGLRNRILFLVIFMKFYNREKELNYLKNYVQLEPNSILFVYGPKSSGKSTVMLRVIEELSKKDDLVFFYYDLREYATPTKEEFLEIFFEKGDKKYLLNRFEINLKIFKFGIEEKFDFDNIKLNDVFSKMKESINAVIKDGKKPILIIDELQKLKSIYFNGEGKGDKSLLNELFNLFVHLTKVRHLCHVICLTSDTLFIEEIYRNSTLENTSEYYLIDWLRKESIRNILKEEGFSEEEVDYCLKYLSLPYEISQLINNKKLGLSVEQTIKQWINIERDKILYLISTQKEFEMGKLIDALKLFENKIKVDIKEIIRDNLMDEVKFLIKNEILFYDVMNGIIKPTSVKKWYAIKEVIE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58413</accession>
    <name>SYV_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Valine--tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Valyl-tRNA synthetase</fullName>
            <shortName evidence="1">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">valS</name>
        <name type="ordered locus">MJ1007</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-valine + tRNA(Val) = AMP + diphosphate + L-valyl-tRNA(Val)</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="1">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64425">
        <property type="entry name" value="F64425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870520.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58413"/>
    <dbReference type="SMR" id="Q58413"/>
    <dbReference type="STRING" id="243232.MJ_1007"/>
    <dbReference type="PRIDE" id="Q58413"/>
    <dbReference type="EnsemblBacteria" id="AAB99009">
        <property type="protein sequence ID" value="AAB99009"/>
        <property type="gene ID" value="MJ_1007"/>
    </dbReference>
    <dbReference type="GeneID" id="1451904"/>
    <dbReference type="KEGG" id="mja:MJ_1007"/>
    <dbReference type="eggNOG" id="arCOG00808">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_0_2_2"/>
    <dbReference type="InParanoid" id="Q58413"/>
    <dbReference type="OMA" id="FATKLWN"/>
    <dbReference type="OrthoDB" id="4914at2157"/>
    <dbReference type="PhylomeDB" id="Q58413"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA editing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.740.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="PTHR11946"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="SSF47323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="SSF50677"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106249" description="Valine--tRNA ligase">
        <location>
            <begin position="1"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region">
        <location>
            <begin position="527"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <sequence length="878" mass="102572" checksum="4D78BCA0D1F2022E" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58414</accession>
    <name>ILVE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative branched-chain-amino-acid aminotransferase</fullName>
            <shortName>BCAT</shortName>
            <ecNumber>2.6.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Transaminase B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ilvE</name>
        <name type="ordered locus">MJ1008</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2-oxoglutarate + L-leucine = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2-oxoglutarate + L-isoleucine = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>2-oxoglutarate + L-valine = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64425">
        <property type="entry name" value="G64425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870521.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58414"/>
    <dbReference type="SMR" id="Q58414"/>
    <dbReference type="STRING" id="243232.MJ_1008"/>
    <dbReference type="EnsemblBacteria" id="AAB99010">
        <property type="protein sequence ID" value="AAB99010"/>
        <property type="gene ID" value="MJ_1008"/>
    </dbReference>
    <dbReference type="GeneID" id="1451905"/>
    <dbReference type="KEGG" id="mja:MJ_1008"/>
    <dbReference type="eggNOG" id="arCOG02297">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020844_3_0_2"/>
    <dbReference type="InParanoid" id="Q58414"/>
    <dbReference type="OMA" id="WRGSEMM"/>
    <dbReference type="OrthoDB" id="41340at2157"/>
    <dbReference type="PhylomeDB" id="Q58414"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050048">
        <property type="term" value="F:L-leucine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="SSF56752"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0032">Aminotransferase</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000103290" description="Putative branched-chain-amino-acid aminotransferase">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="2">
        <location>
            <position position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="288" mass="32096" checksum="033DB417DFD89CBC" modified="1996-11-01" version="1">MKIYLNGKFVDEKDAKVSVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKSLCIDIPLTKEEMIDVVLETLRVNNLRDAYIRLVVTRGVGDLGLDPRKCGKPTIFCIAIPMPPLLGEDGIRAITVSVRRLPVDVLNPAVKSLNYLNSVLAKIQANYAGVDEAFLLDDKGFVVEGTGDNIFIVKNGVLKTPPVYQSILKGITRDVVIKLAKEEGIEVVEEPLTLHDLYTADELFITGTAAEIVPVFEIDGRVINNKQVGEITKKLKEKFKDIRTKWGIKVYDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58419</accession>
    <name>PSTA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">pstA</name>
        <name type="ordered locus">MJ1013</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64426">
        <property type="entry name" value="D64426"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870526.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58419"/>
    <dbReference type="SMR" id="Q58419"/>
    <dbReference type="STRING" id="243232.MJ_1013"/>
    <dbReference type="EnsemblBacteria" id="AAB99017">
        <property type="protein sequence ID" value="AAB99017"/>
        <property type="gene ID" value="MJ_1013"/>
    </dbReference>
    <dbReference type="GeneID" id="1451910"/>
    <dbReference type="KEGG" id="mja:MJ_1013"/>
    <dbReference type="eggNOG" id="arCOG00168">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033621_2_0_2"/>
    <dbReference type="InParanoid" id="Q58419"/>
    <dbReference type="OMA" id="GIARWKA"/>
    <dbReference type="OrthoDB" id="86965at2157"/>
    <dbReference type="PhylomeDB" id="Q58419"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:inorganic phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="SSF161098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060206" description="Probable phosphate transport system permease protein PstA">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="2">
        <location>
            <begin position="68"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="281" mass="30249" checksum="A807ECBD406DD45E" modified="1996-11-01" version="1">MSPIKHKTIRMIKDKIFLFIVGALTLLAILPLFHIIISIVEKGLPIIMERGLTFITGTLSEGGIGPAIVGTLMLTFLATLIGLPLAFLAGAYAYEFPNSFIGRATKMLLQIMLEFPTILVGTFVMGMLVVPMGTFSALAGALALALILTPYVAVYTEEAMAEVPKIYKEGGYALGCTRAQVIFKVITKMAKKGILTGILIGMAKVAGETAPLLFTAGGLYEVYPTNPLEPVGAIPLLIYTLVQSPSIEDHQMAWGAALVMLIIFLAIFVPIRYALKDDIKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58444</accession>
    <name>RPO2N_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo2N</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit B''</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo2N</name>
        <name evidence="3" type="synonym">rpoB2</name>
        <name type="ordered locus">MJ1040</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this organism) is implicated in DNA promoter recognition and in nucleotide binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64429">
        <property type="entry name" value="G64429"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58444"/>
    <dbReference type="SMR" id="Q58444"/>
    <dbReference type="STRING" id="243232.MJ_1040"/>
    <dbReference type="EnsemblBacteria" id="AAB99043">
        <property type="protein sequence ID" value="AAB99043"/>
        <property type="gene ID" value="MJ_1040"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1040"/>
    <dbReference type="eggNOG" id="arCOG01762">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_3_2"/>
    <dbReference type="InParanoid" id="Q58444"/>
    <dbReference type="OMA" id="GKICPSE"/>
    <dbReference type="PhylomeDB" id="Q58444"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="PTHR20856"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000074034" description="DNA-directed RNA polymerase subunit Rpo2N">
        <location>
            <begin position="1"/>
            <end position="498"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="B8YB55"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P11513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="8688087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="498" mass="56487" checksum="F57FD38EEEB79298" modified="1997-11-01" version="1">MAIMRELVDAYFKEHGLIDHQIESYNDFVENRLQKIIDEVGYIETEITGGYKVKLGKIKVGKPVIKEADGSIRPITPMEARIRDLTYSVPLYLEMTPIIGEGEDAREGETVEVYIGELPVMLGSKICHLYGKSREELIDLGEDPEDPFGYFIINGTEKVLITQEDLIPNRILCEKAERSGKIVDVAKVFSTRHGFRALCTVERHPDGLLYATFPGMPGQIPLVILMKALGAETDKDIIESIDDERFFMEIVLNIQEIREEHNINSPEDALEFIGKRVAPGQAKDYRLKRAETVLCNYLLPHLGVTKEDFPKKIRFLGIMARNALELYFGYRGEDDKDHYAYKRAKLAGDLMEDLFRYAFSQLVKDIKYQLERQTLRNKTPSIQAAVRSDILTERIKHAMATGTWVGGKTGVSQLLDRTSYLATNSQLRRIVSPLSRSQPHFEARELHGTHWGKICPSETPEGPNCGLVKNFAIMCKVTREEDDSKVIELLKSFGINVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q58450</accession>
    <name>KAD6_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative adenylate kinase</fullName>
            <shortName evidence="1">AK</shortName>
            <ecNumber evidence="1">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1050</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64431">
        <property type="entry name" value="A64431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870563.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58450"/>
    <dbReference type="SMR" id="Q58450"/>
    <dbReference type="STRING" id="243232.MJ_1050"/>
    <dbReference type="PRIDE" id="Q58450"/>
    <dbReference type="EnsemblBacteria" id="AAB99053">
        <property type="protein sequence ID" value="AAB99053"/>
        <property type="gene ID" value="MJ_1050"/>
    </dbReference>
    <dbReference type="GeneID" id="1451947"/>
    <dbReference type="KEGG" id="mja:MJ_1050"/>
    <dbReference type="eggNOG" id="arCOG01038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079096_0_1_2"/>
    <dbReference type="InParanoid" id="Q58450"/>
    <dbReference type="OMA" id="QCEIFGT"/>
    <dbReference type="OrthoDB" id="110798at2157"/>
    <dbReference type="PhylomeDB" id="Q58450"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:adenylate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="PTHR12595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000153906" description="Putative adenylate kinase">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMP" evidence="1">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="1">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
    </evidence>
    <sequence length="177" mass="20690" checksum="F209572AA79CD2F9" modified="1996-11-01" version="1">MRIAITGTPGVGKTTISKVLRDRLGIKVIDITEAVKKYKLYTEKDEDMDSYVIDFEKLEKFIDEIEEKEKTIILDGHVSHLLNPDYIIVLRCNPEIIKERLEKRGYKPKKVLENIQAEILDVCLCESKGKVYEIDTTNRDVENIVDEIIEAIKHKKERKGVVDWTEKYFDYLTLEIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58471</accession>
    <name>Y1071_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1071</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1071</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0977 C-terminal region.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64433">
        <property type="entry name" value="F64433"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870583.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58471"/>
    <dbReference type="STRING" id="243232.MJ_1071"/>
    <dbReference type="PRIDE" id="Q58471"/>
    <dbReference type="EnsemblBacteria" id="AAB99078">
        <property type="protein sequence ID" value="AAB99078"/>
        <property type="gene ID" value="MJ_1071"/>
    </dbReference>
    <dbReference type="GeneID" id="1451967"/>
    <dbReference type="KEGG" id="mja:MJ_1071"/>
    <dbReference type="eggNOG" id="arCOG00433">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_887427_0_0_2"/>
    <dbReference type="OMA" id="YFGKWNI"/>
    <dbReference type="OrthoDB" id="46740at2157"/>
    <dbReference type="PhylomeDB" id="Q58471"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107157" description="Uncharacterized protein MJ1071">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="313" mass="37221" checksum="AE71D9EB59B7C800" modified="1996-11-01" version="1">MAFDEICDEIILNYEDAKDFAYILKLTYLNEFKKLENLNLNKFGIIKKDDLSFYGKNYPLFKSLLFFNEIPVFRGEKESILFLKSIGLSPRITLNSLTYKEKIKLGNEFLKRCINFVPKEYISYIPQLIFGKEYYFRGVCLKEYVSALNGLYKIGKKKKVKKLIINMELPDEKDVKKYKKKLAKKITLFNKKLENYEINYFNLKFNNKNFECQYIYVKQSVWDKILGLFGEGIELKYYPTLVNIAYSSEKVDFLKPFFIFVDKGDISVYAKVPKLIYLKDGLSLNYLNLRGKYVYFGNWEKDKFWEIIERGVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58478</accession>
    <name>Y1078_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0104 membrane protein MJ1078</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1078</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0104 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64434">
        <property type="entry name" value="E64434"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58478"/>
    <dbReference type="STRING" id="243232.MJ_1078"/>
    <dbReference type="EnsemblBacteria" id="AAB99082">
        <property type="protein sequence ID" value="AAB99082"/>
        <property type="gene ID" value="MJ_1078"/>
    </dbReference>
    <dbReference type="GeneID" id="1451974"/>
    <dbReference type="KEGG" id="mja:MJ_1078"/>
    <dbReference type="eggNOG" id="arCOG00899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048072_3_0_2"/>
    <dbReference type="InParanoid" id="Q58478"/>
    <dbReference type="OMA" id="AGPWHFA"/>
    <dbReference type="OrthoDB" id="87593at2157"/>
    <dbReference type="PhylomeDB" id="Q58478"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00374">
        <property type="entry name" value="TIGR00374"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000138104" description="UPF0104 membrane protein MJ1078">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="234" mass="26481" checksum="2929A35F27ED0BE0" modified="1996-11-01" version="1">MFINSLVPAKLGDVYRGYLLKKKTNESISLGVGTVFIERVFDLVAMISLLFISAYLSFKSDIPKEILYSIKWGVIIILFLIILIFGFLIVNSKINLKNKKLEAILMNFEKGLKAVKLNTLPLLITLSFTGWFIEGLTVYFIFLSLNLNLEILFGVFSDLASSLLTAIPLTPSGLGVVEYALIYILKLKNIDYSGAFAVLILYRLISYFSIVLFGAIMFYIVERNILKEPKNEKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58482</accession>
    <name>Y1082_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1082</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1082</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1503.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64435">
        <property type="entry name" value="A64435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870594.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58482"/>
    <dbReference type="SMR" id="Q58482"/>
    <dbReference type="STRING" id="243232.MJ_1082"/>
    <dbReference type="EnsemblBacteria" id="AAB99084">
        <property type="protein sequence ID" value="AAB99084"/>
        <property type="gene ID" value="MJ_1082"/>
    </dbReference>
    <dbReference type="GeneID" id="1451978"/>
    <dbReference type="KEGG" id="mja:MJ_1082"/>
    <dbReference type="eggNOG" id="arCOG02242">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140786_1_0_2"/>
    <dbReference type="InParanoid" id="Q58482"/>
    <dbReference type="OMA" id="EDLMRCI"/>
    <dbReference type="OrthoDB" id="96961at2157"/>
    <dbReference type="PhylomeDB" id="Q58482"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107162" description="Uncharacterized protein MJ1082">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="120" mass="14455" checksum="F40E066CD5198029" modified="1996-11-01" version="1">MKEFIINRLKKFTIEDLMRCILGLQEIEIRVYFDLLENGEGSVLEIAERVNRDRTTVQKALRSLMNCGLVDRRKVTEKVGYKYIYNAVDLDRVSDIIEELLDDWYQNVKKWLTYFRENRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q58488</accession>
    <name>ECFA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Energy-coupling factor transporter ATP-binding protein EcfA</fullName>
            <shortName evidence="1">ECF transporter A component EcfA</shortName>
            <ecNumber evidence="1">3.6.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ecfA</name>
        <name type="synonym">cbiO</name>
        <name type="ordered locus">MJ1088</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. Energy-coupling factor EcfA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64435">
        <property type="entry name" value="G64435"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870600.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58488"/>
    <dbReference type="SMR" id="Q58488"/>
    <dbReference type="STRING" id="243232.MJ_1088"/>
    <dbReference type="EnsemblBacteria" id="AAB99089">
        <property type="protein sequence ID" value="AAB99089"/>
        <property type="gene ID" value="MJ_1088"/>
    </dbReference>
    <dbReference type="GeneID" id="1451984"/>
    <dbReference type="KEGG" id="mja:MJ_1088"/>
    <dbReference type="eggNOG" id="arCOG00202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_22_2"/>
    <dbReference type="InParanoid" id="Q58488"/>
    <dbReference type="OMA" id="YALELCP"/>
    <dbReference type="OrthoDB" id="92866at2157"/>
    <dbReference type="PhylomeDB" id="Q58488"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005876">
        <property type="entry name" value="Co_trans_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01166">
        <property type="entry name" value="cbiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51246">
        <property type="entry name" value="CBIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092141" description="Energy-coupling factor transporter ATP-binding protein EcfA">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="37"/>
            <end position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01710"/>
        </source>
    </evidence>
    <sequence length="279" mass="31121" checksum="7A2DF31BE68CFA81" modified="1996-11-01" version="1">MYIVETKDLYFRYPDGTAVLKGINFKVKKGEMVSLLGPNGAGKSTLFLHFNGILRPTKGEVLIKGKPIKYDKKSLVEVRKTVGLVFQNPDDQIFAPTVKEDVAFGPLNLGLPKEEVEKRVKEALKAVGMEGFENKPPHHLSGGQKKRVAIAGILAMQPEVIVLDEPTAGLDPVGASKIMKLLYDLNKKGMTIIISTHDVDLVPVYADKVYVMYDGKILKEGTPKEVFSDVETIRKANLRLPRVAHLIEILNKKDNIPIEWGFTIGEVRRNIVNYLKEKC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58554</accession>
    <name>Y1154_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1154</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1154</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64444">
        <property type="entry name" value="A64444"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870665.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58554"/>
    <dbReference type="SMR" id="Q58554"/>
    <dbReference type="STRING" id="243232.MJ_1154"/>
    <dbReference type="DNASU" id="1452050"/>
    <dbReference type="EnsemblBacteria" id="AAB99151">
        <property type="protein sequence ID" value="AAB99151"/>
        <property type="gene ID" value="MJ_1154"/>
    </dbReference>
    <dbReference type="GeneID" id="1452050"/>
    <dbReference type="KEGG" id="mja:MJ_1154"/>
    <dbReference type="eggNOG" id="arCOG04430">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026821_3_0_2"/>
    <dbReference type="InParanoid" id="Q58554"/>
    <dbReference type="OMA" id="RRQTPRW"/>
    <dbReference type="OrthoDB" id="11161at2157"/>
    <dbReference type="PhylomeDB" id="Q58554"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107192" description="Uncharacterized protein MJ1154">
        <location>
            <begin position="1"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="50"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01175"/>
        </source>
    </evidence>
    <sequence length="451" mass="53001" checksum="FA71589054E00C84" modified="1996-11-01" version="1">MKVIRDSIHKDIYLDEKELEIIDSEEFQRLRNIKQTGLTYLVYPSANHTRFEHSLGTMFIASKIAEKINADVELTRVSALLHDIGHPPFSHTLEICGYSHEVFGRKKIKHMNLDNFSKSEIIKTLNRKNLEGKIISGDVDADRMDYLLRDSYHTGTAYGMIDLPRILRSITTFESFGKVKIGILKKGIQAIESLLVARHQMYSAVYMHPTVRIADTMIKRAVIKEIQEKNLDIKDLANMDDIALVSFLRISENYLMERIDRRNLYKNLITYSYFDLNPIEKWIFVNLDEKQILSLESRFYEEFGWDIFIDIYPIPKMEEHNVYIISDEGVKRLDEVSPLAQSLKPSEMRLWNISIYAPKEKIKELRENNVKDRINKILKELDVKVESKLIDILKEYGTITGKRRFLEIAKERGISPKEFYNELHKLIFCGLIKERFNRRTYVYCLNNFVKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58567</accession>
    <name>FWDG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Polyferredoxin protein FwdG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">fwdG</name>
        <name type="ordered locus">MJ1167</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="3">Binds 2 [4Fe-4S] clusters.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64445">
        <property type="entry name" value="F64445"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870680.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58567"/>
    <dbReference type="SMR" id="Q58567"/>
    <dbReference type="STRING" id="243232.MJ_1167"/>
    <dbReference type="EnsemblBacteria" id="AAB99169">
        <property type="protein sequence ID" value="AAB99169"/>
        <property type="gene ID" value="MJ_1167"/>
    </dbReference>
    <dbReference type="GeneID" id="1452065"/>
    <dbReference type="KEGG" id="mja:MJ_1167"/>
    <dbReference type="eggNOG" id="arCOG00292">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139698_5_4_2"/>
    <dbReference type="InParanoid" id="Q58567"/>
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    <dbReference type="OrthoDB" id="118147at2157"/>
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            <fullName>Uncharacterized protein MJ1225</fullName>
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
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                <person name="Fraser C.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
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                <person name="Gocayne J.D."/>
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                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
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                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
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                <person name="Glodek A."/>
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                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
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        <subcellularLocation>
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        <text evidence="3">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily.</text>
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        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002360_6_4_2"/>
    <dbReference type="InParanoid" id="Q58623"/>
    <dbReference type="OMA" id="GVDINWM"/>
    <dbReference type="PhylomeDB" id="Q58623"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008553">
        <property type="term" value="F:P-type proton-exporting transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02076">
        <property type="entry name" value="P-type_ATPase_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
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    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006534">
        <property type="entry name" value="P-type_ATPase_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00120">
        <property type="entry name" value="HATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="SSF81653"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="SSF81660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="SSF81665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01647">
        <property type="entry name" value="ATPase-IIIA_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000046427" description="Putative cation-transporting ATPase MJ1226">
        <location>
            <begin position="1"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="615"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="641"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="680"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="712"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="747"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="773"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="1">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="805" mass="89618" checksum="8E63D3F7E47668CD" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q58624</accession>
    <name>Y1227_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative glycyl-radical enzyme activating enzyme MJ1227</fullName>
            <shortName evidence="3">GRE activating enzyme MJ1227</shortName>
            <ecNumber evidence="1">1.97.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1227</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>glycyl-[protein] + reduced [flavodoxin] + S-adenosyl-L-methionine = 5'-deoxyadenosine + glycin-2-yl radical-[protein] + H(+) + L-methionine + semiquinone [flavodoxin]</text>
            <dbReference type="Rhea" id="RHEA:61976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10622"/>
            <dbReference type="Rhea" id="RHEA-COMP:14480"/>
            <dbReference type="Rhea" id="RHEA-COMP:15993"/>
            <dbReference type="Rhea" id="RHEA-COMP:15994"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29947"/>
            <dbReference type="ChEBI" id="CHEBI:32722"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:140311"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the organic radical-activating enzymes family.</text>
    </comment>
    <dbReference type="EC" id="1.97.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64453">
        <property type="entry name" value="B64453"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870739.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58624"/>
    <dbReference type="SMR" id="Q58624"/>
    <dbReference type="STRING" id="243232.MJ_1227"/>
    <dbReference type="PRIDE" id="Q58624"/>
    <dbReference type="DNASU" id="1452123"/>
    <dbReference type="EnsemblBacteria" id="AAB99230">
        <property type="protein sequence ID" value="AAB99230"/>
        <property type="gene ID" value="MJ_1227"/>
    </dbReference>
    <dbReference type="GeneID" id="1452123"/>
    <dbReference type="KEGG" id="mja:MJ_1227"/>
    <dbReference type="eggNOG" id="arCOG00952">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078147_2_1_2"/>
    <dbReference type="InParanoid" id="Q58624"/>
    <dbReference type="OMA" id="MDIKAPL"/>
    <dbReference type="OrthoDB" id="101349at2157"/>
    <dbReference type="PhylomeDB" id="Q58624"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012840">
        <property type="entry name" value="NrdG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001989">
        <property type="entry name" value="Radical_activat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01094">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02495">
        <property type="entry name" value="NrdG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01087">
        <property type="entry name" value="RADICAL_ACTIVATING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000200545" description="Putative glycyl-radical enzyme activating enzyme MJ1227">
        <location>
            <begin position="1"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="14"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P0A9N4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="240" mass="27682" checksum="362C14E1F761E57A" modified="1996-11-01" version="1">MKALVSGIVDLSTIDYPKKASAVIFLYGCNMKCPYCHNLKFMLEHKRGMTVEEIFNDIDFLFADAIVISGGEPTLQKDAVIEIARYAKEKGFPVKIDTNGTHPEVIEELIKNKLIDYVAIDVKCRFDKYKEFVKCREDGEEIKNKILKIIDLCKKNNVFVECRTTFVPKVMDEEDIEDIAKTVKDCDLYAIQQFEPKDAYDEEFKKLPMPKENELRELGKIAKKYIDNVVIRTINGTFEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58633</accession>
    <name>Y1236_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1236</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1236</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64454">
        <property type="entry name" value="C64454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870748.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58633"/>
    <dbReference type="SMR" id="Q58633"/>
    <dbReference type="STRING" id="243232.MJ_1236"/>
    <dbReference type="EnsemblBacteria" id="AAB99240">
        <property type="protein sequence ID" value="AAB99240"/>
        <property type="gene ID" value="MJ_1236"/>
    </dbReference>
    <dbReference type="GeneID" id="1452132"/>
    <dbReference type="KEGG" id="mja:MJ_1236"/>
    <dbReference type="eggNOG" id="arCOG00543">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009673_5_1_2"/>
    <dbReference type="InParanoid" id="Q58633"/>
    <dbReference type="OMA" id="YLDGMIW"/>
    <dbReference type="OrthoDB" id="5321at2157"/>
    <dbReference type="PhylomeDB" id="Q58633"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="KH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107231" description="Uncharacterized protein MJ1236">
        <location>
            <begin position="1"/>
            <end position="634"/>
        </location>
    </feature>
    <sequence length="634" mass="71761" checksum="F6FB9D6C07732A15" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q58637</accession>
    <name>Y1240_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1240</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1240</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64454">
        <property type="entry name" value="G64454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870752.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58637"/>
    <dbReference type="SMR" id="Q58637"/>
    <dbReference type="STRING" id="243232.MJ_1240"/>
    <dbReference type="EnsemblBacteria" id="AAB99245">
        <property type="protein sequence ID" value="AAB99245"/>
        <property type="gene ID" value="MJ_1240"/>
    </dbReference>
    <dbReference type="GeneID" id="1452136"/>
    <dbReference type="KEGG" id="mja:MJ_1240"/>
    <dbReference type="HOGENOM" id="CLU_2340194_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107233" description="Uncharacterized protein MJ1240">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <sequence length="97" mass="11401" checksum="2F4F613EA65576D7" modified="1996-11-01" version="1">MEIKKSYKDYKKLVWNVSTEFTQALARIMFFYIVNDLETAKNLAKITSPYLPKVPSKLLKELSEAIEEEIKAKSDIEKEKAKEKVKKAFVKLFYYTV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q58642</accession>
    <name>Y1245_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1245</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1245</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ1244 and M.thermoautotrophicum MTH1110.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64455">
        <property type="entry name" value="D64455"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870757.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58642"/>
    <dbReference type="SMR" id="Q58642"/>
    <dbReference type="STRING" id="243232.MJ_1245"/>
    <dbReference type="EnsemblBacteria" id="AAB99257">
        <property type="protein sequence ID" value="AAB99257"/>
        <property type="gene ID" value="MJ_1245"/>
    </dbReference>
    <dbReference type="GeneID" id="1452141"/>
    <dbReference type="KEGG" id="mja:MJ_1245"/>
    <dbReference type="eggNOG" id="arCOG03424">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145179_0_0_2"/>
    <dbReference type="OMA" id="NERWTVM"/>
    <dbReference type="OrthoDB" id="115161at2157"/>
    <dbReference type="PhylomeDB" id="Q58642"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024184">
        <property type="entry name" value="UCP005637"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019296">
        <property type="entry name" value="Unchr_N-regulatory-PII-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10126">
        <property type="entry name" value="Nit_Regul_Hom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005637">
        <property type="entry name" value="UCP005637"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107236" description="Uncharacterized protein MJ1245">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="112" mass="13105" checksum="F605E8244C6C5B71" modified="1996-11-01" version="1">MTKRVLFELFVEEKNVGKAINIMTLAGITGFFLHKYRGLSPDKFKNLSKEELEDIEKVYEIIRDESDKAVVIGTVVKEEKAKKIEELLKEKMNNERWTVMKIPILKVKVHRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58653</accession>
    <name>COFD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">2-phospho-L-lactate transferase</fullName>
            <ecNumber evidence="1">2.7.8.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">LPPG:FO 2-phospho-L-lactate transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cofD</name>
        <name type="ordered locus">MJ1256</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Biochemistry" volume="41" first="3754" last="3761">
            <title>Characterization of the 2-phospho-L-lactate transferase enzyme involved in coenzyme F(420) biosynthesis in Methanococcus jannaschii.</title>
            <authorList>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="11888293"/>
            <dbReference type="DOI" id="10.1021/bi011937v"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF SER-211</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the transfer of the 2-phospholactate moiety from (2S)-lactyl-2-diphospho-5'-guanosine to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of oxidized coenzyme F420-0 and GMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + H(+) + oxidized coenzyme F420-0</text>
            <dbReference type="Rhea" id="RHEA:63444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="EC" id="2.7.8.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Inhibited by EDTA in vitro.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">32 uM for FO</KM>
            <KM evidence="2">17 uM for LPPG</KM>
            <KM evidence="2">515 uM for LPPA</KM>
            <Vmax evidence="2">1.4 umol/min/mg enzyme with LPPG as substrate</Vmax>
            <Vmax evidence="2">0.1 umol/min/mg enzyme with LPPA as substrate</Vmax>
        </kinetics>
        <temperatureDependence>
            <text>Optimum temperature is about 37 degrees Celsius. Thermostable. Still fully active after heating at 80 degrees Celsius for 24 hours. Activity begins to decrease after heating at 98 degrees Celsius for 30 minutes. Inactive after heating at 110 degrees Celsius for 30 minutes.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CofD family.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.28" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64456">
        <property type="entry name" value="G64456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870769.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58653"/>
    <dbReference type="SMR" id="Q58653"/>
    <dbReference type="STRING" id="243232.MJ_1256"/>
    <dbReference type="EnsemblBacteria" id="AAB99260">
        <property type="protein sequence ID" value="AAB99260"/>
        <property type="gene ID" value="MJ_1256"/>
    </dbReference>
    <dbReference type="GeneID" id="1452154"/>
    <dbReference type="KEGG" id="mja:MJ_1256"/>
    <dbReference type="eggNOG" id="arCOG04395">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055795_1_0_2"/>
    <dbReference type="InParanoid" id="Q58653"/>
    <dbReference type="OMA" id="DLDTVMY"/>
    <dbReference type="OrthoDB" id="31831at2157"/>
    <dbReference type="PhylomeDB" id="Q58653"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-12181"/>
    <dbReference type="BRENDA" id="2.7.8.28">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58653"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07186">
        <property type="entry name" value="CofD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01257">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010115">
        <property type="entry name" value="FbiA/CofD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007">
        <property type="entry name" value="PTHR43007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="SSF142338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01819">
        <property type="entry name" value="F420_cofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000145772" description="2-phospho-L-lactate transferase">
        <location>
            <begin position="1"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="No change in activity." evidence="2">
        <original>S</original>
        <variation>A</variation>
        <location>
            <position position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11888293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11888293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="311" mass="34599" checksum="A2830E42C824F64F" modified="1996-11-01" version="1">MIFVITVLSGGTGTPKLLQGLKRVVNNEELAVIVNTGEDTWIGDLYLSPDVDTVLYTLADLINEETWYGVKEDTFYTHEQLKNLGFDEVLRIGDKDRALKMHKTYYLKRGHKLSEVVDMEKVALGIKAKVIPMTDDRVETKILAKVDGKVDLLKFHDFWVKRKGDVEVLDVIYENSLYAKPCEKAVEAIKNSDLVIIGPSNPITSIGPILSLNGIKELLKDKKVVVVSPIVGNSAVSGPAGKLMKAKGYDVSVKGIYEFYKDIVDVLVIDNVDKEIAKEIPCEVLITNTIMKTLDDKVRLAKNIIEFCGSL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-08-03" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58654</accession>
    <name>Y1257_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1257</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1257</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64456">
        <property type="entry name" value="H64456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870770.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58654"/>
    <dbReference type="SMR" id="Q58654"/>
    <dbReference type="STRING" id="243232.MJ_1257"/>
    <dbReference type="PRIDE" id="Q58654"/>
    <dbReference type="EnsemblBacteria" id="AAB99268">
        <property type="protein sequence ID" value="AAB99268"/>
        <property type="gene ID" value="MJ_1257"/>
    </dbReference>
    <dbReference type="GeneID" id="1452155"/>
    <dbReference type="KEGG" id="mja:MJ_1257"/>
    <dbReference type="eggNOG" id="arCOG00056">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_775255_0_0_2"/>
    <dbReference type="InParanoid" id="Q58654"/>
    <dbReference type="OMA" id="NCVVDIG"/>
    <dbReference type="OrthoDB" id="40605at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107243" description="Uncharacterized protein MJ1257">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="THUMP" evidence="1">
        <location>
            <begin position="51"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <sequence length="349" mass="41198" checksum="D0A061A05D8CA5C7" modified="1996-11-01" version="1">MKPVALVTTKPGFEPQLREELNKLPIKKKILWTPFRGILKVLSQNPYEFLNIIKENKNNLKFSLRIIPLEIGCQTDINEIKKAISFLINKKKEKLKNKSFVVRCNRRGNHEFTSEELERIIGEYVLENFKDLNLRVNLKDWDFKINIEILQDESYISIFQDEFNELVIEENIKNLKNLKRYIERPLNRSERKMQELMEKFPFIFENINCVVDIGSSPGGWAKMLSKKAKKVYAIDTGELKIKANNIIHIKKRAENVDFEKDINEEIDLITNDTNLYPDESLFLTLKFAKHLKTNGYIIHTLKARNLKTKKEDLEKVLKILSYYRNIKIFKIINLRANTKNELTLILKKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="69" xmlns="http://uniprot.org/uniprot">
    <accession>Q58688</accession>
    <name>Y1292_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1292</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1292</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58688"/>
    <dbReference type="STRING" id="243232.MJ_1292"/>
    <dbReference type="EnsemblBacteria" id="AAB99308">
        <property type="protein sequence ID" value="AAB99308"/>
        <property type="gene ID" value="MJ_1292"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1292"/>
    <dbReference type="eggNOG" id="arCOG05057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_493165_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107258" description="Uncharacterized protein MJ1292">
        <location>
            <begin position="1"/>
            <end position="552"/>
        </location>
    </feature>
    <sequence length="552" mass="63978" checksum="A4B5B14354B29159" modified="1998-06-01" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58691</accession>
    <name>Y1295_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1295</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1295</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64461">
        <property type="entry name" value="F64461"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870812.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58691"/>
    <dbReference type="SMR" id="Q58691"/>
    <dbReference type="STRING" id="243232.MJ_1295"/>
    <dbReference type="DNASU" id="1452197"/>
    <dbReference type="EnsemblBacteria" id="AAB99302">
        <property type="protein sequence ID" value="AAB99302"/>
        <property type="gene ID" value="MJ_1295"/>
    </dbReference>
    <dbReference type="GeneID" id="1452197"/>
    <dbReference type="KEGG" id="mja:MJ_1295"/>
    <dbReference type="eggNOG" id="arCOG00304">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106253_0_0_2"/>
    <dbReference type="InParanoid" id="Q58691"/>
    <dbReference type="OMA" id="GHNITNG"/>
    <dbReference type="OrthoDB" id="85944at2157"/>
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    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' exodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107261" description="Uncharacterized protein MJ1295">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <sequence length="218" mass="24666" checksum="92C3E7FB2526D0AD" modified="1996-11-01" version="1">MRFDFHTHTVFSDGELIPAELVRRARVLKHRAIAITDHADFSNYKELIEKTTIAKEELKKYWDDIIVIVGVELTHIPPKSIPKMAKKAKDLGAEIVVVHGETVVEPVEEKTNYYASISEDVDILAHPGFIDKETAENLKENDIFVEITSRRGHNITNGYVANIAREFGLKTLINTDTHAPEDLIDDEFAKKVGLGAGLTNKELENTLLHYPKELLKRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58749</accession>
    <name>Y1354_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1354</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1354</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64469">
        <property type="entry name" value="A64469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870871.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58749"/>
    <dbReference type="SMR" id="Q58749"/>
    <dbReference type="STRING" id="243232.MJ_1354"/>
    <dbReference type="EnsemblBacteria" id="AAB99366">
        <property type="protein sequence ID" value="AAB99366"/>
        <property type="gene ID" value="MJ_1354"/>
    </dbReference>
    <dbReference type="GeneID" id="1452256"/>
    <dbReference type="KEGG" id="mja:MJ_1354"/>
    <dbReference type="eggNOG" id="arCOG09680">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1870746_0_0_2"/>
    <dbReference type="OMA" id="EMEKYRY"/>
    <dbReference type="OrthoDB" id="115852at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107293" description="Uncharacterized protein MJ1354">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="145" mass="16713" checksum="15EFEE71C5262B37" modified="1996-11-01" version="1">MDVGIILGILSAMGFLVFLGIGGHILIGYEIIRKISKAYEKGEDVKELENKIINKSHLTNTLEKITTFTLTSIFLFEMEKYRYVIDVGYSILFLVTLTLYLVPNLSLLVWVTFFGATVFMIMLWILRFRAIKEFNKAFIEELTTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58757</accession>
    <name>Y1362_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1362</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1362</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64470">
        <property type="entry name" value="A64470"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870879.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58757"/>
    <dbReference type="SMR" id="Q58757"/>
    <dbReference type="STRING" id="243232.MJ_1362"/>
    <dbReference type="EnsemblBacteria" id="AAB99370">
        <property type="protein sequence ID" value="AAB99370"/>
        <property type="gene ID" value="MJ_1362"/>
    </dbReference>
    <dbReference type="GeneID" id="1452264"/>
    <dbReference type="KEGG" id="mja:MJ_1362"/>
    <dbReference type="eggNOG" id="arCOG01545">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830576_0_0_2"/>
    <dbReference type="OMA" id="FPFYLAT"/>
    <dbReference type="OrthoDB" id="88886at2157"/>
    <dbReference type="PhylomeDB" id="Q58757"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107299" description="Uncharacterized protein MJ1362">
        <location>
            <begin position="1"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="322" mass="35835" checksum="CDF313E6C717C90A" modified="1996-11-01" version="1">MIDELISIIGIPALAFAISTYIPGIQRKIEARIQQRIGPSILAPGFWAFFKFLFKETKAPDANLPKLYNLLPLLSIVVLWALLSITSLTSFHILSNEIGIVGLLKLEEMMYVILGSLAFSIMGWKMPFIDECKGTPFIKTLKLSLEQLGAVRSFKMITIGSFPFYLATFLPFVQKKSIFLKDIVGEPFLFSLAGIFGAACYFIGYVIMIKEYPFSITHTKADVIEGPTMELIAKYRALYLASKELLLIALGSLFATLYLGIAPDIENPITIVENFAIALIFPILATFVRAFSPVLLFKQIYPISYVATLIGVIGFIFALLGW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q58799</accession>
    <name>Y1404_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1404</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1404</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64475">
        <property type="entry name" value="C64475"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58799"/>
    <dbReference type="SMR" id="Q58799"/>
    <dbReference type="STRING" id="243232.MJ_1404"/>
    <dbReference type="EnsemblBacteria" id="AAB99421">
        <property type="protein sequence ID" value="AAB99421"/>
        <property type="gene ID" value="MJ_1404"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1404"/>
    <dbReference type="eggNOG" id="arCOG00601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_656595_0_0_2"/>
    <dbReference type="InParanoid" id="Q58799"/>
    <dbReference type="OMA" id="ETWGAIQ"/>
    <dbReference type="PhylomeDB" id="Q58799"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014651">
        <property type="entry name" value="UCP036983_2CBS_MJ1404"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036983">
        <property type="entry name" value="UCP_2CBS_MJ1404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="SSF54631"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0129">CBS domain</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000107313" description="Uncharacterized protein MJ1404">
        <location>
            <begin position="1"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 1" evidence="1">
        <location>
            <begin position="13"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 2" evidence="1">
        <location>
            <begin position="74"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 3" evidence="1">
        <location>
            <begin position="139"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="CBS 4" evidence="1">
        <location>
            <begin position="217"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <sequence length="421" mass="47609" checksum="FF89C9E64060202F" modified="1996-11-01" version="1">MRLMLNEPVKEIMTKDVVTVTPDTPVSKALGIMEENGFHHLIVVDKKDGKEEYYLISMRDLLLASSTDEEVRSLMYKAHCVHEDTPFLDAVCEMLDSGQRAAPIVNNVGKMVGIITDYDIMARAAKSKIMKDTKVTKIMTRNVITINENDSIGKARALMRDNNIGRLVVVDDEGNPVGMVTEVDILKKVFKPKKKMTAGEFKGEKVPRMGQPVRLIMNTPLITVDVDASAADAARVMQEYDIRGVPVVKGKSLRGIVTRLDIIKYIADLKKGAMIEIELHGMLDPEFKDLAERIIATEVKKMVKHAGKIHWIKITIKKERDKGGVPYYRITTYVKTPNKLYVGEGRPKASLPNKLEAEGEDIAYVSEHERWEFIDVLKESLESVLRQLEADYDKYHPKHAGKVVKGQFPEEFNPEEFKKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-08-03" version="143" xmlns="http://uniprot.org/uniprot">
    <accession>Q58812</accession>
    <name>LONB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Archaeal Lon protease</fullName>
            <ecNumber>3.4.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ATP-dependent protease La homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1417</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2004" name="J. Biol. Chem." volume="279" first="53451" last="53457">
            <title>The active site of a lon protease from Methanococcus jannaschii distinctly differs from the canonical catalytic Dyad of Lon proteases.</title>
            <authorList>
                <person name="Im Y.J."/>
                <person name="Na Y."/>
                <person name="Kang G.B."/>
                <person name="Rho S.H."/>
                <person name="Kim M.K."/>
                <person name="Lee J.H."/>
                <person name="Chung C.H."/>
                <person name="Eom S.H."/>
            </authorList>
            <dbReference type="PubMed" id="15456757"/>
            <dbReference type="DOI" id="10.1074/jbc.M410437200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 456-640</scope>
        <scope>ACTIVE SITE</scope>
    </reference>
    <comment type="function">
        <text evidence="1">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S16 family. Archaeal LonB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64476">
        <property type="entry name" value="H64476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870934.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1XHK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B=456-640"/>
    </dbReference>
    <dbReference type="PDBsum" id="1XHK"/>
    <dbReference type="AlphaFoldDB" id="Q58812"/>
    <dbReference type="SMR" id="Q58812"/>
    <dbReference type="STRING" id="243232.MJ_1417"/>
    <dbReference type="MEROPS" id="S16.008"/>
    <dbReference type="EnsemblBacteria" id="AAB99427">
        <property type="protein sequence ID" value="AAB99427"/>
        <property type="gene ID" value="MJ_1417"/>
    </dbReference>
    <dbReference type="GeneID" id="1452320"/>
    <dbReference type="KEGG" id="mja:MJ_1417"/>
    <dbReference type="eggNOG" id="arCOG02160">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_392630_0_0_2"/>
    <dbReference type="InParanoid" id="Q58812"/>
    <dbReference type="OMA" id="GLYFWNL"/>
    <dbReference type="OrthoDB" id="7657at2157"/>
    <dbReference type="PhylomeDB" id="Q58812"/>
    <dbReference type="EvolutionaryTrace" id="Q58812"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041699">
        <property type="entry name" value="AAA_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004663">
        <property type="entry name" value="Lon_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="PTHR10046"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13654">
        <property type="entry name" value="AAA_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00764">
        <property type="entry name" value="lon_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0720">Serine protease</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000076150" description="Archaeal Lon protease">
        <location>
            <begin position="1"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="2">
        <location>
            <begin position="136"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="topological domain" description="Cytoplasmic" evidence="2">
        <location>
            <begin position="160"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="3">
        <location>
            <begin position="456"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="550"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="593"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="47"/>
            <end position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="461"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="469"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="490"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="498"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="533"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="545"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="548"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="570"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="583"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="603"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="609"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="620"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="629"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="15456757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="1XHK"/>
        </source>
    </evidence>
    <sequence length="649" mass="71918" checksum="2419207160114DBB" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2022-05-25" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58814</accession>
    <name>Y1419_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0147 protein MJ1419</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1419</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0147 family.</text>
    </comment>
    <comment type="sequence caution" evidence="1">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99429" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99429.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64477">
        <property type="entry name" value="B64477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_012980461.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58814"/>
    <dbReference type="SMR" id="Q58814"/>
    <dbReference type="STRING" id="243232.MJ_1419"/>
    <dbReference type="EnsemblBacteria" id="AAB99429">
        <property type="protein sequence ID" value="AAB99429"/>
        <property type="gene ID" value="MJ_1419"/>
    </dbReference>
    <dbReference type="GeneID" id="8804713"/>
    <dbReference type="KEGG" id="mja:MJ_1419"/>
    <dbReference type="eggNOG" id="arCOG04308">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165882_1_0_2"/>
    <dbReference type="InParanoid" id="Q58814"/>
    <dbReference type="OMA" id="RTIIWEV"/>
    <dbReference type="OrthoDB" id="116790at2157"/>
    <dbReference type="PhylomeDB" id="Q58814"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000150908" description="UPF0147 protein MJ1419">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="93" mass="10278" checksum="87088824EB02CD8C" modified="2001-04-27" version="2">MVFGSAASEKTPEEILKGVALMLDEIINDTTVPRNIRAAAEKAKEAVLKEGEEPIVRSATAIHILDEISNDPNMPLHTRTQIWSIVSELERVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q58841</accession>
    <name>PYRK_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Dihydroorotate oxidase B, electron transfer subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrK</name>
        <name type="ordered locus">MJ1446</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="1">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="1">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PyrK family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64480">
        <property type="entry name" value="E64480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870966.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58841"/>
    <dbReference type="SMR" id="Q58841"/>
    <dbReference type="STRING" id="243232.MJ_1446"/>
    <dbReference type="DNASU" id="1452350"/>
    <dbReference type="EnsemblBacteria" id="AAB99455">
        <property type="protein sequence ID" value="AAB99455"/>
        <property type="gene ID" value="MJ_1446"/>
    </dbReference>
    <dbReference type="GeneID" id="1452350"/>
    <dbReference type="KEGG" id="mja:MJ_1446"/>
    <dbReference type="eggNOG" id="arCOG02199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003827_1_1_2"/>
    <dbReference type="InParanoid" id="Q58841"/>
    <dbReference type="OMA" id="RYMKCGI"/>
    <dbReference type="OrthoDB" id="67226at2157"/>
    <dbReference type="PhylomeDB" id="Q58841"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01211">
        <property type="entry name" value="DHODB_Fe_S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037117">
        <property type="entry name" value="Dihydroorotate_DH_ele_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023455">
        <property type="entry name" value="Dihydroorotate_DHASE_ETsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="SSF52343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="SSF63380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0274">FAD</keyword>
    <keyword id="KW-0285">Flavoprotein</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000148375" description="Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="1">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01211"/>
        </source>
    </evidence>
    <sequence length="257" mass="28908" checksum="08D463BFE1CD432D" modified="1996-11-01" version="1">MEKPVICRIKEIIEESPTVKTFVVDKDFDFKPGQFAMLWLPGVDEKPFGFSSKNSFSVARVGEFTKKMHELKEGDIIGVRGPYGTYFEPIGDKVLAVAGGIGAAPIITAVEEFSKQGIEITTILGARTKEELLFLDRFEKVSRLEICTDDGSFGFKGFTTEKMKEVLKEEKFDLIITCGPEIMMKKVVEIANEYNIPVQVSMERYMKCGIGICGQCCVDDEGLCVCKDGPVFWGDKLKFIREFGKYKRDASGKKIYY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58843</accession>
    <name>MTM2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II methyltransferase M.MjaII</fullName>
            <shortName evidence="1">M.MjaII</shortName>
            <ecNumber>2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N-4 cytosine-specific methyltransferase MjaII</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaIIM</name>
        <name type="ordered locus">MJ1448</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="patent" date="1999-03-11" number="WO9911821">
            <title>Method for screening restriction endonucleases.</title>
            <authorList>
                <person name="Noren C.J."/>
                <person name="Roberts R.J."/>
                <person name="Patti J."/>
                <person name="Byrd D.R."/>
                <person name="Morgan R.D."/>
            </authorList>
        </citation>
        <scope>CHARACTERIZATION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">An alpha subtype methylase that recognizes the double-stranded sequence 5'-GGNCC-3', methylates C-5 on both strands, and protects the DNA from cleavage by the MjaII endonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64480">
        <property type="entry name" value="G64480"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870968.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58843"/>
    <dbReference type="STRING" id="243232.MJ_1448"/>
    <dbReference type="REBASE" id="3896">
        <property type="entry name" value="M.MjaII"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99457">
        <property type="protein sequence ID" value="AAB99457"/>
        <property type="gene ID" value="MJ_1448"/>
    </dbReference>
    <dbReference type="GeneID" id="1452352"/>
    <dbReference type="KEGG" id="mja:MJ_1448"/>
    <dbReference type="eggNOG" id="arCOG00890">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039482_0_0_2"/>
    <dbReference type="InParanoid" id="Q58843"/>
    <dbReference type="OMA" id="KHGKICK"/>
    <dbReference type="OrthoDB" id="63009at2157"/>
    <dbReference type="PhylomeDB" id="Q58843"/>
    <dbReference type="PRO" id="PR:Q58843"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000087937" description="Type II methyltransferase M.MjaII">
        <location>
            <begin position="1"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0000305" key="3">
        <source ref="2"/>
    </evidence>
    <sequence length="530" mass="63755" checksum="FF6F29ADD080060C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-04-26" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58877</accession>
    <name>PGK2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">2-phosphoglycerate kinase</fullName>
            <shortName evidence="1">2PGK</shortName>
            <ecNumber evidence="1">2.7.2.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pgk2</name>
        <name type="ordered locus">MJ1482</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate. Involved in the biosynthesis of cyclic 2,3-bisphosphoglycerate, a thermoprotectant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate + ATP = (2R)-2,3-bisphosphoglycerate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42408"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Thermoadapter biosynthesis; cyclic 2,3-diphosphoglycerate biosynthesis; cyclic 2,3-diphosphoglycerate from 2-phospho-D-glycerate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 2-phosphoglycerate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.16" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64485">
        <property type="entry name" value="A64485"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871004.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58877"/>
    <dbReference type="SMR" id="Q58877"/>
    <dbReference type="STRING" id="243232.MJ_1482"/>
    <dbReference type="EnsemblBacteria" id="AAB99494">
        <property type="protein sequence ID" value="AAB99494"/>
        <property type="gene ID" value="MJ_1482"/>
    </dbReference>
    <dbReference type="GeneID" id="1452388"/>
    <dbReference type="KEGG" id="mja:MJ_1482"/>
    <dbReference type="eggNOG" id="arCOG01967">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_848909_0_0_2"/>
    <dbReference type="InParanoid" id="Q58877"/>
    <dbReference type="OMA" id="EMPFSKG"/>
    <dbReference type="OrthoDB" id="57277at2157"/>
    <dbReference type="PhylomeDB" id="Q58877"/>
    <dbReference type="UniPathway" id="UPA00551">
        <property type="reaction ID" value="UER00609"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016774">
        <property type="term" value="F:phosphotransferase activity, carboxyl group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00769">
        <property type="entry name" value="2PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020872">
        <property type="entry name" value="2PKG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156149" description="2-phosphoglycerate kinase">
        <location>
            <begin position="1"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="1">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00769"/>
        </source>
    </evidence>
    <sequence length="309" mass="35545" checksum="0F2528DB92E76278" modified="1996-11-01" version="1">MDLQNDIIVRGKSYEMPFSKGILARSLTAAGLKPSIAYRIAWDIYEMLKKENIRVIDKADLRRRVYYYLISKNYDEVAKKYLLWRMVLGRRPIVILIGGASGVGTSTIAFEIASRLGIPSVIGTDSIREVMRKVISRDLIPTLYESSYTAWKVLRDDEGNKYIKGFERHSEAVLTGVEGVIDRCLVEGQSVIIEGTHLVPTLLKDKYLENSHVVFIMLTIYNEELHKMRFYARGRVSSRPTERYLKYFKIIRMINDYMVETAKKKGIPVVENIKISETVDKCLNIITERLKTMIELEGLSEEDMLEEGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-19" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58921</accession>
    <name>Y1526_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0280 protein MJ1526</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1526</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0280 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64490">
        <property type="entry name" value="E64490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871050.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58921"/>
    <dbReference type="SMR" id="Q58921"/>
    <dbReference type="STRING" id="243232.MJ_1526"/>
    <dbReference type="EnsemblBacteria" id="AAB99544">
        <property type="protein sequence ID" value="AAB99544"/>
        <property type="gene ID" value="MJ_1526"/>
    </dbReference>
    <dbReference type="GeneID" id="1452434"/>
    <dbReference type="KEGG" id="mja:MJ_1526"/>
    <dbReference type="eggNOG" id="arCOG04376">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074757_0_0_2"/>
    <dbReference type="InParanoid" id="Q58921"/>
    <dbReference type="OMA" id="YGVCTSS"/>
    <dbReference type="OrthoDB" id="81284at2157"/>
    <dbReference type="PhylomeDB" id="Q58921"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01079">
        <property type="entry name" value="UPF0280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037456">
        <property type="entry name" value="MA1715-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007183">
        <property type="entry name" value="UPF0280"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006421">
        <property type="entry name" value="UCP006421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="SSF143631"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140486" description="UPF0280 protein MJ1526">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01079"/>
        </source>
    </evidence>
    <sequence length="244" mass="26804" checksum="051C70F1D9C6E3D4" modified="1996-11-01" version="1">MWFKKRIIIKETNILLKVDDKGYFKKAEEIILKNRLELERYILKNPYFLTSYFPVDVEDDAPEIVRLMAIAGEIANVGPMASVAGAIAEMLIKNLNAKNIIAENGGDICLRAKKDVIIGLYAGNSKITGEVGFRLKKEKIKNIYGVCTSSATVGHSVSFGEADAVTVFAKSSAIADAAATAICNASRGRDEEEMINNALEKADEIKKIDGIFVVVKDKVGIKGKIPELVKTDKRITLGELFDIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58956</accession>
    <name>Y1561_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1561</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1561</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0795 and MJ1506.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64494">
        <property type="entry name" value="H64494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871085.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58956"/>
    <dbReference type="SMR" id="Q58956"/>
    <dbReference type="STRING" id="243232.MJ_1561"/>
    <dbReference type="EnsemblBacteria" id="AAB99582">
        <property type="protein sequence ID" value="AAB99582"/>
        <property type="gene ID" value="MJ_1561"/>
    </dbReference>
    <dbReference type="GeneID" id="1452469"/>
    <dbReference type="KEGG" id="mja:MJ_1561"/>
    <dbReference type="eggNOG" id="arCOG02080">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036466_0_0_2"/>
    <dbReference type="InParanoid" id="Q58956"/>
    <dbReference type="OMA" id="DSWSNCN"/>
    <dbReference type="OrthoDB" id="20421at2157"/>
    <dbReference type="PhylomeDB" id="Q58956"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107412" description="Uncharacterized protein MJ1561">
        <location>
            <begin position="1"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="524"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="553" mass="61713" checksum="86CA1E67C5A0AA01" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58957</accession>
    <name>Y1562_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative membrane protein MJ1562</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1562</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the resistance-nodulation-cell division (RND) (TC 2.A.6) family. MmpL subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64495">
        <property type="entry name" value="A64495"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58957"/>
    <dbReference type="SMR" id="Q58957"/>
    <dbReference type="STRING" id="243232.MJ_1562"/>
    <dbReference type="TCDB" id="2.A.6.7.2">
        <property type="family name" value="the resistance-nodulation-cell division (rnd) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99583">
        <property type="protein sequence ID" value="AAB99583"/>
        <property type="gene ID" value="MJ_1562"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1562"/>
    <dbReference type="eggNOG" id="arCOG02174">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060248_0_0_2"/>
    <dbReference type="InParanoid" id="Q58957"/>
    <dbReference type="OMA" id="EFGGTET"/>
    <dbReference type="PhylomeDB" id="Q58957"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004869">
        <property type="entry name" value="MMPL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000731">
        <property type="entry name" value="SSD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03176">
        <property type="entry name" value="MMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50156">
        <property type="entry name" value="SSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000103587" description="Putative membrane protein MJ1562">
        <location>
            <begin position="1"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="388" mass="42770" checksum="40B048F80AE24D54" modified="1996-11-01" version="1">MVVLMLREILKKVAHFSEQKPFLMLLIILIITVFAGISATNVKSQTAFEKMLPQDNPIIKTLYEVRDEFGGTDVITICIKLKPSDSSDKVVDIRDPRVLKAIKELEDNLRYVDGITSVSSPVDIIIQKNNGIVPNDIDTVKDILNKLPEDKRKRIFNSDYSMTVVNAYTDAGGDQKKLMRVMDDVNERIEETPFPPGVEVIATGTPPMRKLMDELMKESQSFTTTVGLIGILIILIIYFRKPLSSIMPLLPVLIAVIWTGGAMGLLDIPLDMATAGIGSLILGLGIDYGIHLMHRYDEERRKGMPIDKAIETAVVETGTAVMATTATTVVGFLALVLAPLPMMANLGKVCALGISFCMVVVLTLLPALIVIEERHIMPLIKRLKGDTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58981</accession>
    <name>NNR_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Bifunctional NAD(P)H-hydrate repair enzyme Nnr</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName>ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber>4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>NAD(P)H-hydrate epimerase</fullName>
                <ecNumber>5.1.99.6</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName>NAD(P)HX epimerase</fullName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name type="primary">nnr</name>
        <name type="ordered locus">MJ1586</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6S)-NADHX + ADP = AMP + H(+) + NADH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6S)-NADPHX + ADP = AMP + H(+) + NADPH + phosphate</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="1">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136"/>
    <dbReference type="EC" id="5.1.99.6"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64498">
        <property type="entry name" value="A64498"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58981"/>
    <dbReference type="SMR" id="Q58981"/>
    <dbReference type="STRING" id="243232.MJ_1586"/>
    <dbReference type="EnsemblBacteria" id="AAB99605">
        <property type="protein sequence ID" value="AAB99605"/>
        <property type="gene ID" value="MJ_1586"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1586"/>
    <dbReference type="eggNOG" id="arCOG00018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024853_4_1_2"/>
    <dbReference type="InParanoid" id="Q58981"/>
    <dbReference type="OMA" id="HNGGDAL"/>
    <dbReference type="PhylomeDB" id="Q58981"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NADHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052857">
        <property type="term" value="F:NADPHX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017953">
        <property type="entry name" value="Carbohydrate_kinase_pred_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="SSF64153"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01049">
        <property type="entry name" value="YJEF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01050">
        <property type="entry name" value="YJEF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000119051" description="Bifunctional NAD(P)H-hydrate repair enzyme Nnr">
        <location>
            <begin position="1"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal">
        <location>
            <begin position="35"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal">
        <location>
            <begin position="232"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="NAD(P)H-hydrate epimerase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX 1; for epimerase activity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX 1; for epimerase activity" evidence="1">
        <location>
            <begin position="158"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="ADP-dependent (S)-NAD(P)H-hydrate dehydratase" evidence="1">
        <location>
            <begin position="232"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADPHX 2; for dehydratase activity" evidence="1">
        <location>
            <begin position="380"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <label>1</label>
            <note>for epimerase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <label>2</label>
            <note>for dehydratase activity</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="404"/>
            <end position="408"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <label>2</label>
            <note>for dehydratase activity</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="491" mass="54979" checksum="6F8994706685F6F2" modified="1996-11-01" version="1">MRRDINNFLFGERMELFEILKQKIKEKEVITPKEMAIIDDNAEFLGIQKILLMENAGKAVYEEIKDIDAEEFIIFCGTGNNGGDGFVVARHLGKGDVILIGKESEIKTYEARENFKILKNLAEFGNIRIREIKWAEEVNDIFERLKNKKAVIIDAMIGTGVKGELREPFKTIVDKINELKQINKNIFVISVDVETGHLESDLTITFHKRKTINKDNAIVKKIGIPKEAEYIVGWGDLKALRKRDSNSHKGQNGKVLIIGGSKDFYGAPILAGLAALKIVDLVGILSVGKVIDKVNHPEFIMYRVEGDYLSSQHVDYTLEIAKKYDVVVLGNGLGANNRTKAFLNEFLAKYDGKVVIDADAIKVIDYNNFEFSENYIFTPHKREFEYMGIDLDNIENIKSTIVLKGKYDIIFNANNLKINKTGNAGLTKGGTGDVLAGLIGALFAVNEAFLSACCGAFINGYAGDLLLKEKGYYYTPLDLIEKIPNVLKIFQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58995</accession>
    <name>CDPAS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CDP-archaeol synthase</fullName>
            <ecNumber evidence="1">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">carS</name>
        <name type="ordered locus">MJ1600</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64499">
        <property type="entry name" value="G64499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871125.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58995"/>
    <dbReference type="SMR" id="Q58995"/>
    <dbReference type="STRING" id="243232.MJ_1600"/>
    <dbReference type="EnsemblBacteria" id="AAB99620">
        <property type="protein sequence ID" value="AAB99620"/>
        <property type="gene ID" value="MJ_1600"/>
    </dbReference>
    <dbReference type="GeneID" id="1452509"/>
    <dbReference type="KEGG" id="mja:MJ_1600"/>
    <dbReference type="eggNOG" id="arCOG04106">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105710_0_0_2"/>
    <dbReference type="InParanoid" id="Q58995"/>
    <dbReference type="OMA" id="GKTWRGT"/>
    <dbReference type="OrthoDB" id="124973at2157"/>
    <dbReference type="PhylomeDB" id="Q58995"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="PTHR39650"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000094170" description="CDP-archaeol synthase">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <sequence length="177" mass="19460" checksum="2F10ABA3951396E2" modified="1996-11-01" version="1">MFYRLLFASLWYILPAYVANASACIFGGGTPVDLGKNFIDGRRLIGNGVTYRGCIFGILCGTLVGLIQGILVDFNIFNSLDFYGTVLDHVILAFFLSVGAIVGDAVGSFIKRRLNIERGKPAPLLDQLDFVIGALAFGYIVAPIPYEMIIIICLFTVFVHLLGNIIAYKLGIKDVWW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58996</accession>
    <name>Y1601_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1601</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1601</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64499">
        <property type="entry name" value="H64499"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871126.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58996"/>
    <dbReference type="STRING" id="243232.MJ_1601"/>
    <dbReference type="EnsemblBacteria" id="AAB99621">
        <property type="protein sequence ID" value="AAB99621"/>
        <property type="gene ID" value="MJ_1601"/>
    </dbReference>
    <dbReference type="GeneID" id="1452510"/>
    <dbReference type="KEGG" id="mja:MJ_1601"/>
    <dbReference type="eggNOG" id="arCOG04396">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094842_1_0_2"/>
    <dbReference type="InParanoid" id="Q58996"/>
    <dbReference type="OMA" id="CPPDRFD"/>
    <dbReference type="OrthoDB" id="104907at2157"/>
    <dbReference type="PhylomeDB" id="Q58996"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107434" description="Uncharacterized protein MJ1601">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01007"/>
        </source>
    </evidence>
    <sequence length="160" mass="18337" checksum="0886905973D1D0EA" modified="1996-11-01" version="1">MITIDIELKDKPGELLRVLTPISKYGANVISIIHSREEKRGGKVPVRIVIDVDDKDKLKKILEDLEKEGAIIKKIDGKDKKVYLDVVVIGHVVDTNVRDTIDRINEIGLVEDLDLIMPHPDKESSAMMRIIVDEDKIEELFYLFEELEKEKGLLFIKSIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-08-03" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q59010</accession>
    <name>Y1615_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1615</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1615</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64501">
        <property type="entry name" value="F64501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871140.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59010"/>
    <dbReference type="STRING" id="243232.MJ_1615"/>
    <dbReference type="EnsemblBacteria" id="AAB99637">
        <property type="protein sequence ID" value="AAB99637"/>
        <property type="gene ID" value="MJ_1615"/>
    </dbReference>
    <dbReference type="GeneID" id="1452524"/>
    <dbReference type="KEGG" id="mja:MJ_1615"/>
    <dbReference type="eggNOG" id="arCOG02394">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067019_0_0_2"/>
    <dbReference type="InParanoid" id="Q59010"/>
    <dbReference type="OMA" id="HVGILRY"/>
    <dbReference type="OrthoDB" id="38718at2157"/>
    <dbReference type="PhylomeDB" id="Q59010"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007381">
        <property type="entry name" value="CheF1/F2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201">
        <property type="entry name" value="PTHR42201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04283">
        <property type="entry name" value="CheF-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107438" description="Uncharacterized protein MJ1615">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <sequence length="255" mass="29634" checksum="A40BF51E0F469B3E" modified="1996-11-01" version="1">MIDKSSEIARFSGKGILITPKTLEKPLLKWEKLEIILYKDKIVFEFVDKTIEVGVEDIEDVGAELPKKVIDIAKSTLEDITYHSSIIIKSKEFGNVMVGFAPETSIYGKAPIDNFLRKLFYILLNKKEVKILYNAGENSENTKWENGFLTFIKKRIKDGLVTKIEYRLVVEILDNEDSKIYDIFSNIKDVEIEEKDVDGEIEPVLKILQVKDGKDIISYLYTKDKKVRLFILRYMVILLDYKYIGILRYLQETVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q60334</accession>
    <name>ARCH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0024</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archease family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64302">
        <property type="entry name" value="H64302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869516.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60334"/>
    <dbReference type="SMR" id="Q60334"/>
    <dbReference type="STRING" id="243232.MJ_0024"/>
    <dbReference type="EnsemblBacteria" id="AAB98005">
        <property type="protein sequence ID" value="AAB98005"/>
        <property type="gene ID" value="MJ_0024"/>
    </dbReference>
    <dbReference type="GeneID" id="1450862"/>
    <dbReference type="KEGG" id="mja:MJ_0024"/>
    <dbReference type="eggNOG" id="arCOG04055">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_111362_3_0_2"/>
    <dbReference type="InParanoid" id="Q60334"/>
    <dbReference type="OMA" id="WLSELLY"/>
    <dbReference type="OrthoDB" id="125788at2157"/>
    <dbReference type="PhylomeDB" id="Q60334"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="PTHR12682"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="SSF69819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0106">Calcium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000068843" description="Protein archease">
        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <sequence length="139" mass="16411" checksum="9FD640383110CEA6" modified="1997-11-01" version="1">MFNYFETTADLGVEAKGKSLEEAFKEGAKGLYNIMVDIDKVDKKEKIEFEITGEDLEELLYNFLNELLFYTDVENLVFNDFDVKIEKNDNGYRLKCTAYGEKINKEKHNIKEEVKAVTYHKMEIKQEEDGWKIRYIVDL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q60361</accession>
    <name>Y054_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0054</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0054</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64306">
        <property type="entry name" value="F64306"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869546.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60361"/>
    <dbReference type="STRING" id="243232.MJ_0054"/>
    <dbReference type="EnsemblBacteria" id="AAB98034">
        <property type="protein sequence ID" value="AAB98034"/>
        <property type="gene ID" value="MJ_0054"/>
    </dbReference>
    <dbReference type="GeneID" id="1450893"/>
    <dbReference type="KEGG" id="mja:MJ_0054"/>
    <dbReference type="eggNOG" id="arCOG04893">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_982163_0_0_2"/>
    <dbReference type="InParanoid" id="Q60361"/>
    <dbReference type="OMA" id="QGLCGRI"/>
    <dbReference type="OrthoDB" id="45819at2157"/>
    <dbReference type="PhylomeDB" id="Q60361"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009181">
        <property type="entry name" value="UCP004929_methan"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09872">
        <property type="entry name" value="DUF2099"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004929">
        <property type="entry name" value="UCP004929"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03275">
        <property type="entry name" value="methan_mark_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106672" description="Uncharacterized protein MJ0054">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <sequence length="281" mass="31169" checksum="ABDB1F83C578B7B3" modified="1997-11-01" version="1">MDRHVMEALGKARVVVENGRVVEVTEPKIKYCPLFAKHRGIKEITKESIKENIEFRIKDFGLFTKNRVVEESRYIVPFGASEILMSALKRKAIDVAVIVADCAGTIITSNPNLVQGLCGRISGIIETSPILEVIEKIEKAGGVVLNKKTAEINQFEGVKKAIELDYKKIAVTVTNLEDAKRCKSLENDEIKILTFGVHLTGIEGSEEIAKYFDLVTACASKVLREKLKGKIKAQIGKTIPIFALSDFGKEILLERAKDLDKVLISIENLPVLNDNQPKPLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q60385</accession>
    <name>Y078_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0078</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0078</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64309">
        <property type="entry name" value="F64309"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869570.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60385"/>
    <dbReference type="SMR" id="Q60385"/>
    <dbReference type="STRING" id="243232.MJ_0078"/>
    <dbReference type="EnsemblBacteria" id="AAB98066">
        <property type="protein sequence ID" value="AAB98066"/>
        <property type="gene ID" value="MJ_0078"/>
    </dbReference>
    <dbReference type="GeneID" id="1450917"/>
    <dbReference type="KEGG" id="mja:MJ_0078"/>
    <dbReference type="eggNOG" id="arCOG08278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1313129_0_0_2"/>
    <dbReference type="OMA" id="SEFECIR"/>
    <dbReference type="OrthoDB" id="70540at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106684" description="Uncharacterized protein MJ0078">
        <location>
            <begin position="1"/>
            <end position="207"/>
        </location>
    </feature>
    <sequence length="207" mass="24563" checksum="571955A95E51DAF1" modified="1997-11-01" version="1">MNCEEYRKKLKLNYGNREKLKVLKELYQMEVDEITKLNILDDVFELLTSTKERGFEDIISTHYTSDSKNKAIIYYCIKIIEKVGIKYPNLVYIYIPYLIKLLDSEFECIRFASAEALANIPSKLTIYAYPKLIKKLDNEVYAKVLVKLIMKSDNKEAILLKLFENFNEYSLYVIKELYKYDKELVYEFIPLILKEFGNNGLYSFLQK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>O53113</accession>
    <name>Y409_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0409</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0409</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64351">
        <property type="entry name" value="A64351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869908.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="O53113"/>
    <dbReference type="STRING" id="243232.MJ_0409"/>
    <dbReference type="EnsemblBacteria" id="AAB98405">
        <property type="protein sequence ID" value="AAB98405"/>
        <property type="gene ID" value="MJ_0409"/>
    </dbReference>
    <dbReference type="GeneID" id="1451269"/>
    <dbReference type="KEGG" id="mja:MJ_0409"/>
    <dbReference type="eggNOG" id="arCOG06686">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024489_0_0_2"/>
    <dbReference type="OMA" id="FAYINKV"/>
    <dbReference type="OrthoDB" id="10251at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106858" description="Uncharacterized protein MJ0409">
        <location>
            <begin position="1"/>
            <end position="703"/>
        </location>
    </feature>
    <sequence length="703" mass="81654" checksum="A74D0DF5037D0545" modified="1998-06-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P54014</accession>
    <name>RL3_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl3</name>
        <name type="ordered locus">MJ0176</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64322">
        <property type="entry name" value="A64322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869671.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54014"/>
    <dbReference type="SMR" id="P54014"/>
    <dbReference type="STRING" id="243232.MJ_0176"/>
    <dbReference type="EnsemblBacteria" id="AAB98161">
        <property type="protein sequence ID" value="AAB98161"/>
        <property type="gene ID" value="MJ_0176"/>
    </dbReference>
    <dbReference type="GeneID" id="1451023"/>
    <dbReference type="KEGG" id="mja:MJ_0176"/>
    <dbReference type="eggNOG" id="arCOG04070">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033361_2_0_2"/>
    <dbReference type="InParanoid" id="P54014"/>
    <dbReference type="OMA" id="HQRTEYN"/>
    <dbReference type="OrthoDB" id="31722at2157"/>
    <dbReference type="PhylomeDB" id="P54014"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_L3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_L3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_L3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_L3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="PTHR11363"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="SSF50447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000077210" description="50S ribosomal protein L3">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <sequence length="335" mass="38117" checksum="63241BDE7DEF81C3" modified="1996-10-01" version="1">MGLNINRPRRGSLAFSPRKRAKRPVPRIRSWPEEDTVRLQAFPVYKAGMSHAFIKEDNPKSPNAGQEVFTPITILEAPPINVCAIRVYGRNERNYLTTLTEVWADNLDKELERKIKLPKKEDRKTVEDLEALKDKIEDVRVLVHTNPKLTCLPKKKPEILEIRIGGKDIEERLNYAKEILGKQLNITDVFQEGELVDTIGVTKGKGFQGQVKRWGVKIQFGKHARKGVGRHVGSIGPWQPKMVMWSVPMPGQMGYHQRTEYNKRILKIGNNGDEITPKGGFLHYGVIRNNYVVLKGSVQGPAKRLIVLRRAIRPQEPLIKVPEITYISTTSKQGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>P54061</accession>
    <name>RL30E_METJA</name>
    <protein>
        <recommendedName>
            <fullName>50S ribosomal protein L30e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rpl30e</name>
        <name type="ordered locus">MJ1044</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64430">
        <property type="entry name" value="C64430"/>
    </dbReference>
    <dbReference type="PDB" id="3CPQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.90 A"/>
        <property type="chains" value="A/B=1-110"/>
    </dbReference>
    <dbReference type="PDBsum" id="3CPQ"/>
    <dbReference type="AlphaFoldDB" id="P54061"/>
    <dbReference type="SMR" id="P54061"/>
    <dbReference type="STRING" id="243232.MJ_1044"/>
    <dbReference type="EnsemblBacteria" id="AAB99048">
        <property type="protein sequence ID" value="AAB99048"/>
        <property type="gene ID" value="MJ_1044"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1044"/>
    <dbReference type="eggNOG" id="arCOG01752">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130502_1_0_2"/>
    <dbReference type="InParanoid" id="P54061"/>
    <dbReference type="OMA" id="RPHKIMA"/>
    <dbReference type="PhylomeDB" id="P54061"/>
    <dbReference type="EvolutionaryTrace" id="P54061"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_L30e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="L30/L30e/YlxQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="L30e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_L30e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_L30e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_L7Ae/L30e/S12e/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="PTHR11449"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449:SF1">
        <property type="entry name" value="PTHR11449:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="SSF55315"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000146147" description="50S ribosomal protein L30e">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="20"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="26"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="39"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="66"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="73"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="87"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="99"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3CPQ"/>
        </source>
    </evidence>
    <sequence length="110" mass="12134" checksum="EBECA4E135E098B6" modified="1996-10-01" version="1">MRRRENMDVNKAIRTAVDTGKVILGSKRTIKFVKHGEGKLVVLAGNIPKDLEEDVKYYAKLSNIPVYQHKITSLELGAVCGKPFPVAALLVLDEGLSNIMELVEKKEGGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>P54062</accession>
    <name>RS12_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps12</name>
        <name type="ordered locus">MJ1046</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870559.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54062"/>
    <dbReference type="SMR" id="P54062"/>
    <dbReference type="STRING" id="243232.MJ_1046"/>
    <dbReference type="EnsemblBacteria" id="AAB99050">
        <property type="protein sequence ID" value="AAB99050"/>
        <property type="gene ID" value="MJ_1046"/>
    </dbReference>
    <dbReference type="GeneID" id="24891505"/>
    <dbReference type="KEGG" id="mja:MJ_1046"/>
    <dbReference type="eggNOG" id="arCOG04255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115574_0_1_2"/>
    <dbReference type="InParanoid" id="P54062"/>
    <dbReference type="OMA" id="KFRWSQR"/>
    <dbReference type="OrthoDB" id="85293at2157"/>
    <dbReference type="PhylomeDB" id="P54062"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_S12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_S12/S23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_S12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_S23_euk/arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="PTHR11652"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00055">
        <property type="entry name" value="RIBOSOMAL_S12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000146369" description="30S ribosomal protein S12">
        <location>
            <begin position="1"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <sequence length="148" mass="16829" checksum="6261744BDED62CFD" modified="1998-07-15" version="2">MSGSKSPRGEFAGRKLRLKRKWCRWHDYNYVRRVLKLKEKYDPLEGAPMARGIVIEKVGLEAKQPNSAIRKCVRVQLIKNGRVVTAFCPGNHAINFIDEHDEVIIEGIGGPKGPRAKGDIPGVKYKVIMVGRNSLRELVRGRQEKIKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>P54063</accession>
    <name>RS7_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps7</name>
        <name type="ordered locus">MJ1047</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64430">
        <property type="entry name" value="F64430"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870560.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54063"/>
    <dbReference type="SMR" id="P54063"/>
    <dbReference type="STRING" id="243232.MJ_1047"/>
    <dbReference type="EnsemblBacteria" id="AAB99051">
        <property type="protein sequence ID" value="AAB99051"/>
        <property type="gene ID" value="MJ_1047"/>
    </dbReference>
    <dbReference type="GeneID" id="1451944"/>
    <dbReference type="KEGG" id="mja:MJ_1047"/>
    <dbReference type="eggNOG" id="arCOG04254">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063975_0_0_2"/>
    <dbReference type="InParanoid" id="P54063"/>
    <dbReference type="OMA" id="KMNIVER"/>
    <dbReference type="OrthoDB" id="80498at2157"/>
    <dbReference type="PhylomeDB" id="P54063"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_S7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_S5/S7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_S5/S7_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_S7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_S7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_S7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_S7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="PTHR11205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="SSF47973"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000124399" description="30S ribosomal protein S7">
        <location>
            <begin position="1"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <sequence length="191" mass="21760" checksum="98A03F368921FD97" modified="1996-10-01" version="1">MELDEIKVFGRWSTKDVVVKDPGLRNYINLTPIYVPHTAGRYTKRQFEKAKMNIVERLVNKVMRREENTGKKLKALKIVENAFEIIEKRTKQNPIQVLVDAIENAGPREDTTRISYGGIVYLQSVDCSSLRRIDVALRNIALGAYMAAHKSKKPIEEALAEEIIAAARGDMQKSYAVRKKEETERVAQSAR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>P81316</accession>
    <name>YB5A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1155.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1155.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To A.fulgidus AF1754.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870667.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81316"/>
    <dbReference type="SMR" id="P81316"/>
    <dbReference type="STRING" id="243232.MJ_1155.1"/>
    <dbReference type="DNASU" id="1452052"/>
    <dbReference type="EnsemblBacteria" id="AAB99154">
        <property type="protein sequence ID" value="AAB99154"/>
        <property type="gene ID" value="MJ_1155.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1452052"/>
    <dbReference type="KEGG" id="mja:MJ_1155.1"/>
    <dbReference type="eggNOG" id="arCOG07899">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_172997_0_0_2"/>
    <dbReference type="OMA" id="WENFGIS"/>
    <dbReference type="OrthoDB" id="130327at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008407">
        <property type="entry name" value="Brnchd-chn_aa_trnsp_AzlD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05437">
        <property type="entry name" value="AzlD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107193" description="Uncharacterized protein MJ1155.1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="110" mass="12498" checksum="47AFCF07E9448A17" modified="1998-07-15" version="1">MDKNILAIIFVAVGTYLIRYIPIHLHSKIKNIDEKVKEINEILIYSSTSVISALFITSFIKFPIIFSNVLISTISLIFAIVSYKKWNNLGISILISVVIYYLASKFLISI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>P82736</accession>
    <name>Y658_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0658</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0658</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH238.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870163.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P82736"/>
    <dbReference type="STRING" id="243232.MJ_0658"/>
    <dbReference type="EnsemblBacteria" id="AAB98657">
        <property type="protein sequence ID" value="AAB98657"/>
        <property type="gene ID" value="MJ_0658"/>
    </dbReference>
    <dbReference type="GeneID" id="1451524"/>
    <dbReference type="KEGG" id="mja:MJ_0658"/>
    <dbReference type="eggNOG" id="arCOG00098">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_867712_0_0_2"/>
    <dbReference type="OMA" id="FTYNFIW"/>
    <dbReference type="OrthoDB" id="67919at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="SSF69336"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106974" description="Uncharacterized protein MJ0658">
        <location>
            <begin position="1"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="318" mass="35714" checksum="92948BAE49469523" modified="2001-03-01" version="1">MLFNIFKKSNKKKLDEFLNENKDKVLTLKLDKPVDCLCDFTYNFIWQSKFNPETEIKENITFKTLVEHLKNGGVVYIKGNVGKRFCSSMGVDLKYFSGSGERIKVGTVIVDGDVDTRFGISMLSGTVYINEKNKIKEPMGNVIEVESDIKGYRKFISITEFVEERYKEEKLLKPNKFDKNKGILEINDKIKRDTVGARLNEDKTIVVNGDVDLSTGILMRKGKVIVNGNAGKNTGAVLNGGTVIINGNTNDFTGFEMRDGIIVVDGNAGKYLGAKKKEGVIYARDGKEVPPTKKYELNNDDIAFLKSLGYTGKFYKFI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q57557</accession>
    <name>Y092_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized iron-sulfur protein MJ0092</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0092</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64311">
        <property type="entry name" value="D64311"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869584.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57557"/>
    <dbReference type="SMR" id="Q57557"/>
    <dbReference type="STRING" id="243232.MJ_0092"/>
    <dbReference type="DNASU" id="1450931"/>
    <dbReference type="EnsemblBacteria" id="AAB98073">
        <property type="protein sequence ID" value="AAB98073"/>
        <property type="gene ID" value="MJ_0092"/>
    </dbReference>
    <dbReference type="GeneID" id="1450931"/>
    <dbReference type="KEGG" id="mja:MJ_0092"/>
    <dbReference type="eggNOG" id="arCOG00333">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023081_2_0_2"/>
    <dbReference type="InParanoid" id="Q57557"/>
    <dbReference type="OMA" id="VYQDACH"/>
    <dbReference type="OrthoDB" id="21885at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
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    </dbReference>
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        <property type="term" value="F:electron transfer activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
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        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
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        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="SSF54292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0001">2Fe-2S</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000158709" description="Uncharacterized iron-sulfur protein MJ0092">
        <location>
            <begin position="1"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="2">
        <location>
            <begin position="2"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="3">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="3">
        <location>
            <begin position="177"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="489" mass="56128" checksum="A634315770434C3D" modified="1997-11-01" version="1">MIKITVKRFNGEKEYLESYEVPENITVLEALEYINKHYEANILFRASCRNAQCGSCAVTINGEPRLACETKVEDGMIIEPLRGFKVIRDLIVDREPYYKKLLGIKNYLIRKNYPEELEILIPKYVEENKELRGCIDCLSCLSVCPAREVSDYPGPTFMRQLARFAFDKRDEDGREITAYFENIYNCTTCAKCVEVCPKEIDIVHRAIEKLRALAFSKGYYIENHLKVRENVLKYNRSVVEEELPLLKQVADFYPAESEKLRVAFFTGCLVDFRLQNVGKDAIKVLNAHGVSVVIPKNQVCCGSPFFRTGQRDVAEMLKRKNLEIFNKLDVDCVVTICAGCGSTLKNDYKERKFEVKDITEVLTEVGLLKYKPLKMRITYHDPCHLRRGQKIYKQPREILKSIPELEFIDIEARCCGAGGGVRSGKPDIANLIGKSRARMIYDANVDAVITVCPFCEYHIRDSLKRFKEENKIDKEIDVMNIVSLLAKVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57574</accession>
    <name>Y110_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0110</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0110</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64313">
        <property type="entry name" value="F64313"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57574"/>
    <dbReference type="SMR" id="Q57574"/>
    <dbReference type="STRING" id="243232.MJ_0110"/>
    <dbReference type="TCDB" id="2.A.123.2.5">
        <property type="family name" value="the sweet, pq-loop, saliva, mtn3 (sweet) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98095">
        <property type="protein sequence ID" value="AAB98095"/>
        <property type="gene ID" value="MJ_0110"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0110"/>
    <dbReference type="eggNOG" id="arCOG05022">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135915_2_1_2"/>
    <dbReference type="InParanoid" id="Q57574"/>
    <dbReference type="OMA" id="WIIYGYK"/>
    <dbReference type="PhylomeDB" id="Q57574"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106697" description="Uncharacterized protein MJ0110">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="93" mass="10727" checksum="5BD1158C16D3D0D8" modified="1996-11-01" version="1">MVINMDFDITVIGYIAGTLTTFASLPQLIKSLKEKDMSNISLAFVITFTTGLTLWLIYGILRNDYPIIVFNILSLMFWIPITYLKIRDEMRKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q57593</accession>
    <name>Y129_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0129</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0129</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0554 and MJ0587.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64316">
        <property type="entry name" value="A64316"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57593"/>
    <dbReference type="SMR" id="Q57593"/>
    <dbReference type="STRING" id="243232.MJ_0129"/>
    <dbReference type="DNASU" id="1450971"/>
    <dbReference type="EnsemblBacteria" id="AAB98118">
        <property type="protein sequence ID" value="AAB98118"/>
        <property type="gene ID" value="MJ_0129"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0129"/>
    <dbReference type="HOGENOM" id="CLU_1718222_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q57593"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035582">
        <property type="entry name" value="DUF5418"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17439">
        <property type="entry name" value="DUF5418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106707" description="Uncharacterized protein MJ0129">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="170" mass="19621" checksum="4F3B62C7DDA89D9A" modified="1997-11-01" version="1">MLSFVVMSFLIFVIVMVNEHKAHLSVIQKMILAVVNGSITIILSIIVFYIFYPQNISLFLITAGILTVFVFLYGLLLFLFGFTHRELSYLSKYDKYKFLCKFTIEMFSSLTNHAFLTISAIVLYQIQHPKPTIDFIVMIGMITISVIVVMLLFLKTYSIIIKQLKKLENN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57597</accession>
    <name>Y133_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0133</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0133</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64316">
        <property type="entry name" value="E64316"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57597"/>
    <dbReference type="SMR" id="Q57597"/>
    <dbReference type="STRING" id="243232.MJ_0133"/>
    <dbReference type="DNASU" id="1450974"/>
    <dbReference type="EnsemblBacteria" id="AAB98114">
        <property type="protein sequence ID" value="AAB98114"/>
        <property type="gene ID" value="MJ_0133"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0133"/>
    <dbReference type="eggNOG" id="arCOG01894">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068832_0_0_2"/>
    <dbReference type="InParanoid" id="Q57597"/>
    <dbReference type="OMA" id="HIHISGI"/>
    <dbReference type="PhylomeDB" id="Q57597"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00019">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="PTHR21445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="SSF51658"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106711" description="Uncharacterized protein MJ0133">
        <location>
            <begin position="1"/>
            <end position="273"/>
        </location>
    </feature>
    <sequence length="273" mass="31386" checksum="87069E801903BC49" modified="1997-11-01" version="1">MNREIMLIFGTAGVPISAEDEFKAVDVLRKLNLGAMELEFVKGVYMKEDYAKKLKEYGEDIIFSAHAPHYINLNANEEEKVENSIRRIIKTAKVLNNCGKNLVFHPGYYLKRSKEVTYNRIKSNIQRILDKLEALNLNVMLRPETTGKTTQFGDIDETLKLCFELNILPCIDFSHIYARSRGVINDYNSFYKILEKVENVLGKEAIKDMHIHLSGIEYGKGGERRHLPLNESNFNYRDVLKALKDFDASGTVICESPMLEYDAVLLMKCYNEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57620</accession>
    <name>ACDB2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Acetyl-CoA decarbonylase/synthase complex subunit beta 2</fullName>
            <shortName>ACDS complex subunit beta 2</shortName>
            <ecNumber evidence="3">2.3.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>ACDS complex acyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cdhC2</name>
        <name type="ordered locus">MJ0156</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon complex generates CO from CO(2), while the beta subunit (this protein) combines the CO with CoA and a methyl group to form acetyl-CoA. The methyl group, which is incorporated into acetyl-CoA, is transferred to the beta subunit by a corrinoid iron-sulfur protein (the gamma-delta complex).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>acetyl-CoA + Co(I)-[corrinoid Fe-S protein] + H(+) = CO + CoA + methyl-Co(III)-[corrinoid Fe-S protein]</text>
            <dbReference type="Rhea" id="RHEA:45212"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.3.1.169"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="1">Binds 1 [Ni-Fe-S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CdhC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.169" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64319">
        <property type="entry name" value="E64319"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869651.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57620"/>
    <dbReference type="SMR" id="Q57620"/>
    <dbReference type="STRING" id="243232.MJ_0156"/>
    <dbReference type="EnsemblBacteria" id="AAB98138">
        <property type="protein sequence ID" value="AAB98138"/>
        <property type="gene ID" value="MJ_0156"/>
    </dbReference>
    <dbReference type="GeneID" id="1451000"/>
    <dbReference type="KEGG" id="mja:MJ_0156"/>
    <dbReference type="eggNOG" id="arCOG04360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_613408_0_0_2"/>
    <dbReference type="InParanoid" id="Q57620"/>
    <dbReference type="OMA" id="FMHLNQR"/>
    <dbReference type="OrthoDB" id="26992at2157"/>
    <dbReference type="PhylomeDB" id="Q57620"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018492">
        <property type="term" value="F:carbon-monoxide dehydrogenase (acceptor) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043884">
        <property type="term" value="F:CO-methylating acetyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01138">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045822">
        <property type="entry name" value="ACS_CODH_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004461">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038571">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023432">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42281">
        <property type="entry name" value="PTHR42281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19436">
        <property type="entry name" value="ACS_CODH_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03598">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="SSF56821"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00316">
        <property type="entry name" value="cdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000155102" description="Acetyl-CoA decarbonylase/synthase complex subunit beta 2">
        <location>
            <begin position="1"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="469" mass="52642" checksum="BDC866AD7C28661C" modified="1996-11-01" version="1">MFDDIPVSVGPMNEGERVRGPDMYVELAGPKSYGFELVKVVNKAEDKVEIIGKDIDEMEEGSRNPFAIIVEVSGSNLEEDLEGVLERRIHEFLNYIEGVMHLNQRDQVWIRINKDSFNKGLRLKHIGKVVQRLFKAEFPFIEKCDVTIITDPEKVKEELEKAREIYNKRDEKTKALHEEDVDVFYGCVMCQSFAPTHVCVITPDRPALCGGINYLDARAAAKIDPNGPIFEIPKGECLDEKLGIYSGVNEVVRERSQGTVEEVTLHSALEKPCTSCGCFEAIVFYIPEVDGFGIAHRGYKGETPMGIPFSTLAGQCSGGKQVPGFVGISISYMKSPKFLQGDGGWERVVWLPKELKERVKDAIPEELYDKIATEEDVKTTDELIKFLKEKGHPCAERIGAEVEEEAIEEEEVEEEMEEVEGIEVPTMTLPGTFAGLPPGIKIVLYNAVIKAEKIIITKEEPEKKKKKKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q57654</accession>
    <name>Y201_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0201</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0201</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64325">
        <property type="entry name" value="B64325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869696.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57654"/>
    <dbReference type="STRING" id="243232.MJ_0201"/>
    <dbReference type="DNASU" id="1451050"/>
    <dbReference type="EnsemblBacteria" id="AAB98185">
        <property type="protein sequence ID" value="AAB98185"/>
        <property type="gene ID" value="MJ_0201"/>
    </dbReference>
    <dbReference type="GeneID" id="1451050"/>
    <dbReference type="KEGG" id="mja:MJ_0201"/>
    <dbReference type="eggNOG" id="arCOG03118">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098634_2_0_2"/>
    <dbReference type="InParanoid" id="Q57654"/>
    <dbReference type="OMA" id="IFQPIPT"/>
    <dbReference type="OrthoDB" id="84854at2157"/>
    <dbReference type="PhylomeDB" id="Q57654"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="SNARE_assoc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="SNARE_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106738" description="Uncharacterized protein MJ0201">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <sequence length="160" mass="18626" checksum="EDEACBF3DA3B24E2" modified="1997-11-01" version="1">MINLELFKEFLLNLIKDYGYFGIFLVGFSEPIFQPFPTEIFIIAGILLGLDWKLVWLISTIACNFGAVVTYYLAKKYGEKLMLKLFDEEKIKKGSHYLKKWGILGVIIASFTPIPFEVICWVCGSFEMPFERYMIAVFLSRLIRHGMVILPFVLKDHIHF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57655</accession>
    <name>Y202_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0202</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0202</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64325">
        <property type="entry name" value="C64325"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57655"/>
    <dbReference type="SMR" id="Q57655"/>
    <dbReference type="STRING" id="243232.MJ_0202"/>
    <dbReference type="EnsemblBacteria" id="AAB98186">
        <property type="protein sequence ID" value="AAB98186"/>
        <property type="gene ID" value="MJ_0202"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0202"/>
    <dbReference type="eggNOG" id="arCOG04410">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071520_1_0_2"/>
    <dbReference type="InParanoid" id="Q57655"/>
    <dbReference type="OMA" id="FLLKAKC"/>
    <dbReference type="PhylomeDB" id="Q57655"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014444">
        <property type="entry name" value="PH1575-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006593">
        <property type="entry name" value="UCP006593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="SSF111321"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106739" description="Uncharacterized protein MJ0202">
        <location>
            <begin position="1"/>
            <end position="304"/>
        </location>
    </feature>
    <sequence length="304" mass="34419" checksum="8A3FA78D5085CA8C" modified="1996-11-01" version="1">MVLLILKFHGESVKIKPECAICIIRQVVDAANEITDDEREQFRLIKSTMEVIKDVYGESAVPAWMGTVVHRYLKKISNNNDPYKNLKEKANKIALQYLDKVREMSNTDDELERLRKKVLATIAGNVIDFGAYSTGINIEKLIEDTLNGELKIDNSRKLLNDLKDKNIKKILYICDNAGEIIFDRVLMEEIKKYDKDIVAVVKGKPILNDATLEDAKIAKIDEIAKVITTGSDIIGIILEECSEEFLKEFESADLIIAKGMGNYESLTEYEDKIDKPIYYILKAKCKPVAENIGVDVGDNVLLKR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q57656</accession>
    <name>PUR5_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber>6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">purM</name>
        <name type="ordered locus">MJ0203</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AIR synthase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64325">
        <property type="entry name" value="D64325"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869698.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57656"/>
    <dbReference type="SMR" id="Q57656"/>
    <dbReference type="STRING" id="243232.MJ_0203"/>
    <dbReference type="PRIDE" id="Q57656"/>
    <dbReference type="EnsemblBacteria" id="AAB98187">
        <property type="protein sequence ID" value="AAB98187"/>
        <property type="gene ID" value="MJ_0203"/>
    </dbReference>
    <dbReference type="GeneID" id="1451052"/>
    <dbReference type="KEGG" id="mja:MJ_0203"/>
    <dbReference type="eggNOG" id="arCOG00639">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047116_0_0_2"/>
    <dbReference type="InParanoid" id="Q57656"/>
    <dbReference type="OMA" id="EPLFMTD"/>
    <dbReference type="OrthoDB" id="62508at2157"/>
    <dbReference type="PhylomeDB" id="Q57656"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="PTHR10520"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="SSF55326"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="SSF56042"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148281" description="Phosphoribosylformylglycinamidine cyclo-ligase">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="350" mass="38202" checksum="C1F68B7884DFB293" modified="1996-11-01" version="1">MVTYKDAGVDISHEDKVIKALVSQITFKRSDIKPAELGLHYAGAVEFGDYYLVLSTDGVGSKMIVAEMANKFDTVGIDMIAMNVNDAICIGAEPIALVDYLAVGHITEEIAEQIGKGLNEGAKEANINIVGGETATLPDMIKGIDLAGTVLAIVKKDEIITGKDVKAGDVIVGLRSSGIHSNGLSLARKVFFDIAKLDINDKLSYGKTVAEELLTPTRIYVKPVLEMIRDKDIEVKGLAHITGGSFRKLKRLNDKVTYYIDNLPEPLPIFKEIQRLGNVPDEEMFRTFNMGIGFCVIVDEEDANKVIKIANKYNIPAQVIGRVVDSLEVNGNKIVGKAVVKYNDKHIILE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57667</accession>
    <name>HYPA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydrogenase maturation factor HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">hypA</name>
        <name type="ordered locus">MJ0214</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the HypA/HybF family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64326">
        <property type="entry name" value="G64326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869710.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57667"/>
    <dbReference type="SMR" id="Q57667"/>
    <dbReference type="STRING" id="243232.MJ_0214"/>
    <dbReference type="EnsemblBacteria" id="AAB98198">
        <property type="protein sequence ID" value="AAB98198"/>
        <property type="gene ID" value="MJ_0214"/>
    </dbReference>
    <dbReference type="GeneID" id="1451064"/>
    <dbReference type="KEGG" id="mja:MJ_0214"/>
    <dbReference type="eggNOG" id="arCOG04426">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126929_2_1_2"/>
    <dbReference type="InParanoid" id="Q57667"/>
    <dbReference type="OMA" id="ILLCPCG"/>
    <dbReference type="OrthoDB" id="109617at2157"/>
    <dbReference type="PhylomeDB" id="Q57667"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00213">
        <property type="entry name" value="HypA_HybF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020538">
        <property type="entry name" value="Hydgase_Ni_incorp_HypA/HybF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000688">
        <property type="entry name" value="HypA/HybF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535">
        <property type="entry name" value="PTHR34535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01155">
        <property type="entry name" value="HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004761">
        <property type="entry name" value="Hydrgn_mat_HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00100">
        <property type="entry name" value="hypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01249">
        <property type="entry name" value="HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000129077" description="Hydrogenase maturation factor HypA">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="124" mass="14058" checksum="1769B05D496957AD" modified="1996-11-01" version="1">MHELSYANAMLEAILNSIKKEEEKGKKIKKVTEINLEVGELTFINVEQLKFAFEVIAEGTVCEGAKINVEFIKPKCKCLDCGYEGEPEILDEFEVYCPKCKSIRLKLSGGKEFNIKNATVEYED</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57671</accession>
    <name>VATF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>V-type ATP synthase subunit F</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>V-ATPase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">atpF</name>
        <name type="ordered locus">MJ0218</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64327">
        <property type="entry name" value="C64327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869714.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57671"/>
    <dbReference type="SMR" id="Q57671"/>
    <dbReference type="STRING" id="243232.MJ_0218"/>
    <dbReference type="EnsemblBacteria" id="AAB98201">
        <property type="protein sequence ID" value="AAB98201"/>
        <property type="gene ID" value="MJ_0218"/>
    </dbReference>
    <dbReference type="GeneID" id="8803838"/>
    <dbReference type="KEGG" id="mja:MJ_0218"/>
    <dbReference type="eggNOG" id="arCOG04102">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135754_2_2_2"/>
    <dbReference type="InParanoid" id="Q57671"/>
    <dbReference type="OMA" id="QIPDKFG"/>
    <dbReference type="OrthoDB" id="113093at2157"/>
    <dbReference type="PhylomeDB" id="Q57671"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00312">
        <property type="entry name" value="ATP_synth_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022944">
        <property type="entry name" value="ATPase_V1-cplx_fsu_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="SSF159468"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144818" description="V-type ATP synthase subunit F">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="98" mass="10941" checksum="5D8680EFEB8FA08F" modified="1996-11-01" version="1">MKVGVVGDRETAIGFRLAGLTDVYEVKNDEEAVKAINELANNENIAFIIITERIAESIKDKLKNINKVIVEIPDKHGKLERIDPVKELIRKAIGVSMK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q57686</accession>
    <name>TRPD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="1">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpD</name>
        <name type="ordered locus">MJ0234</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + N-(5-phospho-beta-D-ribosyl)anthranilate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64329">
        <property type="entry name" value="C64329"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57686"/>
    <dbReference type="SMR" id="Q57686"/>
    <dbReference type="STRING" id="243232.MJ_0234"/>
    <dbReference type="EnsemblBacteria" id="AAB98221">
        <property type="protein sequence ID" value="AAB98221"/>
        <property type="gene ID" value="MJ_0234"/>
    </dbReference>
    <dbReference type="GeneID" id="1451087"/>
    <dbReference type="KEGG" id="mja:MJ_0234"/>
    <dbReference type="eggNOG" id="arCOG02012">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034315_2_1_2"/>
    <dbReference type="InParanoid" id="Q57686"/>
    <dbReference type="OMA" id="GPMTNPA"/>
    <dbReference type="PhylomeDB" id="Q57686"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="PTHR43285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="SSF47648"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="SSF52418"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000154512" description="Anthranilate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="90"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="107"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <sequence length="336" mass="36630" checksum="DF360D25328F71B6" modified="1996-11-01" version="1">MITEALKKVIEFKDLDEKEAEAVMKDIMSGNAKPTQIAAILTALRMKGETIEEITAFAKIMREFSLKINPNVPKLLDTCGTGGDNLNTFNISTATAFVVSAYVPVAKHGNKAVSSKSGSADVLEALGVNLNVPIERVKESIEKIGIGFLFAPHFHPAMKFATPVRKELGIRTVFNVLGPLTNPANANYQLMGVYDEKLTEKLANVLKNLGLKGALVVHGSGMDEITTIGKTKISELRNGEIKSYYIEPEDFGIKKAKLEDIRGGDAEENAKIIGEIFEGEEVGAKRDIVVLNAAFALYIAEEAKDVEEGIKLAEKSIDEGKALKKLEDLIEFYREG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57700</accession>
    <name>PYRF_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="1">4.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">OMP decarboxylase</fullName>
            <shortName evidence="1">OMPDCase</shortName>
            <shortName evidence="1">OMPdecase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrF</name>
        <name type="ordered locus">MJ0252</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>H(+) + orotidine 5'-phosphate = CO2 + UMP</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the OMP decarboxylase family. Type 1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98239" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98239.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64331">
        <property type="entry name" value="E64331"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496436.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4LUI">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A/B=1-213"/>
    </dbReference>
    <dbReference type="PDB" id="4LUJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A/B=1-213"/>
    </dbReference>
    <dbReference type="PDBsum" id="4LUI"/>
    <dbReference type="PDBsum" id="4LUJ"/>
    <dbReference type="AlphaFoldDB" id="Q57700"/>
    <dbReference type="SMR" id="Q57700"/>
    <dbReference type="STRING" id="243232.MJ_0252"/>
    <dbReference type="EnsemblBacteria" id="AAB98239">
        <property type="protein sequence ID" value="AAB98239"/>
        <property type="gene ID" value="MJ_0252"/>
    </dbReference>
    <dbReference type="GeneID" id="1451106"/>
    <dbReference type="KEGG" id="mja:MJ_0252"/>
    <dbReference type="eggNOG" id="arCOG00081">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067069_2_0_2"/>
    <dbReference type="InParanoid" id="Q57700"/>
    <dbReference type="OMA" id="EMSHPGA"/>
    <dbReference type="OrthoDB" id="54384at2157"/>
    <dbReference type="PhylomeDB" id="Q57700"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01200_A">
        <property type="entry name" value="OMPdecase_type1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="PTHR32119:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0210">Decarboxylase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134610" description="Orotidine 5'-phosphate decarboxylase">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="3"/>
            <end position="7"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="28"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="34"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="54"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="81"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="107"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="117"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="140"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="163"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="175"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="187"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="192"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="4LUI"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="4LUJ"/>
        </source>
    </evidence>
    <sequence length="213" mass="23592" checksum="0B073BE37D50C556" modified="2002-01-23" version="2">MPKLMLALDVLDRDRALKIVEDVKDYVDAIKVGYPLVLSTGTEIIKEIKKLCNKEVIADFKVADIPATNEKIAKITLKYADGIIVHGFVGEDSVKAVQDVAKKLNKKVIMVTEMSHPGAVQFLQPIADKLSEMAKKLKVDAIVAPSTRPERLKEIKEIAELPVITPGVGAQGGKIEDILNILDENDYVIVGRAIYQSQNPKEEAKKYKEMLNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q57736</accession>
    <name>Y288_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0288</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0288</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64336">
        <property type="entry name" value="A64336"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869786.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57736"/>
    <dbReference type="STRING" id="243232.MJ_0288"/>
    <dbReference type="DNASU" id="1451143"/>
    <dbReference type="EnsemblBacteria" id="AAB98281">
        <property type="protein sequence ID" value="AAB98281"/>
        <property type="gene ID" value="MJ_0288"/>
    </dbReference>
    <dbReference type="GeneID" id="1451143"/>
    <dbReference type="KEGG" id="mja:MJ_0288"/>
    <dbReference type="eggNOG" id="arCOG09662">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1529261_0_0_2"/>
    <dbReference type="OMA" id="KVAIMVV"/>
    <dbReference type="OrthoDB" id="94914at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106775" description="Uncharacterized protein MJ0288">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="178" mass="19266" checksum="7F0DE50289012476" modified="1996-11-01" version="1">MKKFSAIFGLLSVIFVIMAISQVSGLSGAITPPKIDIMVNASNGLPQDINSIIYVKNPNSFPVKVEMVTTGDLNNSKKVEVKIMKNNFTLKPGETVGVNITFTVKEKDNYEGDILTKISPVDYGDDKKGVNLKASVVLPTKVAIMVVGNEIHTKELVITAVLIISILGLGAMLIRRHL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57753</accession>
    <name>Y305_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0305</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0305</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64338">
        <property type="entry name" value="B64338"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57753"/>
    <dbReference type="SMR" id="Q57753"/>
    <dbReference type="STRING" id="243232.MJ_0305"/>
    <dbReference type="TCDB" id="2.A.49.4.1">
        <property type="family name" value="the chloride carrier/channel (clc) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98292">
        <property type="protein sequence ID" value="AAB98292"/>
        <property type="gene ID" value="MJ_0305"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0305"/>
    <dbReference type="eggNOG" id="arCOG02569">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015263_5_3_2"/>
    <dbReference type="InParanoid" id="Q57753"/>
    <dbReference type="OMA" id="NPYYRVL"/>
    <dbReference type="PhylomeDB" id="Q57753"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="Cl-channel_volt-gated"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="SSF81340"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0868">Chloride</keyword>
    <keyword id="KW-0869">Chloride channel</keyword>
    <keyword id="KW-0407">Ion channel</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000094500" description="Uncharacterized protein MJ0305">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="395" mass="42618" checksum="9CDC65D6201CF7C0" modified="1996-11-01" version="1">MPMNIVNMFGKYIKIIKWIGIASLIGIVGGLSSVIIAIIIEYFPEKHNVLLIPIVFFIAGLFVDYIYELKGSGIDRVLKALNTNEKLTWIRGLLKVLLAGAVIAVGGSAGKEGPCVQSSASFADELYRLLKLKNRELVIITGIAGGLGGAFSAPLGTAILACEIIEHENFNYINLIPPIIASVVGYLIFYLITGRKHLFNITLSYTINIHDFLLFILGAFFCSLIAHCYIKTYRKISSTFDNLKIPYCIKTLIGGILVAVISYFIPEVMGMGLTLTKELFIMEFSLVFLVLLLIGKILATSFTVGSGTPGGLVFPSMCIGAISGIIFGSLIGDCSAPYIVLGIATTLSATTNAPLGGAVLCTEIFGFDFAVPASIGAVIGYQMTKLETIFKYIRF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q57806</accession>
    <name>Y360_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0360</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0360</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64344">
        <property type="entry name" value="H64344"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869859.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57806"/>
    <dbReference type="SMR" id="Q57806"/>
    <dbReference type="STRING" id="243232.MJ_0360"/>
    <dbReference type="PRIDE" id="Q57806"/>
    <dbReference type="EnsemblBacteria" id="AAB98352">
        <property type="protein sequence ID" value="AAB98352"/>
        <property type="gene ID" value="MJ_0360"/>
    </dbReference>
    <dbReference type="GeneID" id="1451217"/>
    <dbReference type="KEGG" id="mja:MJ_0360"/>
    <dbReference type="eggNOG" id="arCOG10172">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881086_0_0_2"/>
    <dbReference type="OrthoDB" id="97924at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106831" description="Uncharacterized protein MJ0360">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="135" mass="14803" checksum="E1938F1628AA213F" modified="1996-11-01" version="1">MKRLGVFLILASIVCGVVAICGCTGGGGTDYSSSTASAETETCPVQILEHHLVRKDYGTVYVEGVAQNVGNKRLKFVEIKARFYDADGVLIDEFMDVHRDVDPGQKFRFKIIGPIGEEGKKVAKYDIAVGTWWTE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57821</accession>
    <name>CAS3_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative CRISPR-associated nuclease/helicase Cas3</fullName>
            <ecNumber>3.1.-.-</ecNumber>
            <ecNumber>3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas3</name>
        <name type="ordered locus">MJ0376</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the N-terminal section; belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">In the central section; belongs to the CRISPR-associated helicase Cas3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">This protein is about 60 residues too short at the N-terminus compared to its close orthologs.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EC" id="3.6.4.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64346">
        <property type="entry name" value="H64346"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869875.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57821"/>
    <dbReference type="SMR" id="Q57821"/>
    <dbReference type="STRING" id="243232.MJ_0376"/>
    <dbReference type="PRIDE" id="Q57821"/>
    <dbReference type="EnsemblBacteria" id="AAB98365">
        <property type="protein sequence ID" value="AAB98365"/>
        <property type="gene ID" value="MJ_0376"/>
    </dbReference>
    <dbReference type="GeneID" id="1451233"/>
    <dbReference type="KEGG" id="mja:MJ_0376"/>
    <dbReference type="eggNOG" id="arCOG01445">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009347_1_1_2"/>
    <dbReference type="InParanoid" id="Q57821"/>
    <dbReference type="OMA" id="WLNIVYD"/>
    <dbReference type="OrthoDB" id="1317at2157"/>
    <dbReference type="PhylomeDB" id="Q57821"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035011">
        <property type="entry name" value="Cas3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF5">
        <property type="entry name" value="PTHR47959:SF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106839" description="Putative CRISPR-associated nuclease/helicase Cas3">
        <location>
            <begin position="1"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="domain" description="HD Cas3-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="3">
        <location>
            <begin position="213"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="4">
        <location>
            <begin position="434"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box">
        <location>
            <begin position="352"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6"/>
    <sequence length="728" mass="86467" checksum="8BC3DBCA30226B40" modified="1998-12-15" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57846</accession>
    <name>Y403_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MEMO1 family protein MJ0403</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0403</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MEMO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64350">
        <property type="entry name" value="C64350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869902.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57846"/>
    <dbReference type="SMR" id="Q57846"/>
    <dbReference type="STRING" id="243232.MJ_0403"/>
    <dbReference type="EnsemblBacteria" id="AAB98390">
        <property type="protein sequence ID" value="AAB98390"/>
        <property type="gene ID" value="MJ_0403"/>
    </dbReference>
    <dbReference type="GeneID" id="1451263"/>
    <dbReference type="KEGG" id="mja:MJ_0403"/>
    <dbReference type="eggNOG" id="arCOG01728">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038085_2_0_2"/>
    <dbReference type="InParanoid" id="Q57846"/>
    <dbReference type="OMA" id="EQEAQYG"/>
    <dbReference type="OrthoDB" id="82745at2157"/>
    <dbReference type="PhylomeDB" id="Q57846"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd07361">
        <property type="entry name" value="MEMO_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00055">
        <property type="entry name" value="MEMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002737">
        <property type="entry name" value="MEMO1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11060">
        <property type="entry name" value="PTHR11060"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01875">
        <property type="entry name" value="Memo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000134379" description="MEMO1 family protein MJ0403">
        <location>
            <begin position="1"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00055"/>
        </source>
    </evidence>
    <sequence length="287" mass="32097" checksum="D788000C3E6B8834" modified="1996-11-01" version="1">MNKIRYPAVAGLFYPSHPDELIDMIEQCYLHKFGPKSMPVHGTYEKPIGLLCPHAGYVYSGPIQAHSYYELSKRVDALEETTVVILGPNHTGLGSGVSVMDGIWRTPLGDVKCDEEFVEELWRKCEIVDLDETAHLNEHSIEVQLPFLKHLELLNIAKFKIVPICMMFQDYETAVEVGYFIAKIAKELNRRIVVIASSDLTHYEPQEIASKKDAIVIKDILEMNEKELYEDVVNYNISMCGYGPVIAMLKAMKTLGAEKAKLLAYATSGDITGDYSAVVGYASAIVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q57851</accession>
    <name>MPTD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">Dihydroneopterin aldolase</fullName>
            <shortName evidence="1 5">DHNA</shortName>
            <ecNumber evidence="1 2 3">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 5">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptD</name>
        <name type="ordered locus">MJ0408</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="ACS Chem. Biol." volume="7" first="1807" last="1816">
            <title>Comparative genomics guided discovery of two missing archaeal enzyme families involved in the biosynthesis of the pterin moiety of tetrahydromethanopterin and tetrahydrofolate.</title>
            <authorList>
                <person name="Crecy-Lagard V.D."/>
                <person name="Phillips G."/>
                <person name="Grochowski L.L."/>
                <person name="Yacoubi B.E."/>
                <person name="Jenney F."/>
                <person name="Adams M.W."/>
                <person name="Murzin A.G."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="22931285"/>
            <dbReference type="DOI" id="10.1021/cb300342u"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>GENE NAME</scope>
        <scope>PATHWAY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2014" name="J. Bacteriol." volume="196" first="3191" last="3198">
            <title>Biochemical characterization of a dihydroneopterin aldolase used for methanopterin biosynthesis in methanogens.</title>
            <authorList>
                <person name="Wang Y."/>
                <person name="Xu H."/>
                <person name="Grochowski L.L."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="24982305"/>
            <dbReference type="DOI" id="10.1128/JB.01812-14"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MUTAGENESIS OF GLU-25; HIS-35; GLN-61; TYR-78 AND TYR-111</scope>
    </reference>
    <reference key="4">
        <citation type="submission" date="2011-07" db="PDB data bank">
            <title>Crystal structure of hypothetical protein MJ0408 from Methanococcus jannaschii.</title>
            <authorList>
                <consortium name="New York structural genomix research consortium (NYSGXRC)"/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.89 ANGSTROMS) OF 2-121 IN COMPLEX WITH PTERIN ANALOG</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Catalyzes the conversion of 7,8-dihydroneopterin (H2Neo) to 6-hydroxymethyl-7,8-dihydropterin (6-HMD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">64.5 uM for 7,8-dihydroneopterin (at 25 degrees Celsius)</KM>
            <KM evidence="3">9.9 uM for 7,8-dihydroneopterin (at 70 degrees Celsius)</KM>
            <text evidence="3">kcat is 0.07 sec(-1) at 25 degrees Celsius. kcat is 1.0 sec(-1) at 70 degrees Celsius.</text>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 8.3.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1 2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal dihydroneopterin aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1 2 3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64350">
        <property type="entry name" value="H64350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869907.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2OGF">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.89 A"/>
        <property type="chains" value="A/B/C/D=2-121"/>
    </dbReference>
    <dbReference type="PDBsum" id="2OGF"/>
    <dbReference type="AlphaFoldDB" id="Q57851"/>
    <dbReference type="SMR" id="Q57851"/>
    <dbReference type="STRING" id="243232.MJ_0408"/>
    <dbReference type="EnsemblBacteria" id="AAB98398">
        <property type="protein sequence ID" value="AAB98398"/>
        <property type="gene ID" value="MJ_0408"/>
    </dbReference>
    <dbReference type="GeneID" id="1451268"/>
    <dbReference type="KEGG" id="mja:MJ_0408"/>
    <dbReference type="eggNOG" id="arCOG04705">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149105_1_0_2"/>
    <dbReference type="InParanoid" id="Q57851"/>
    <dbReference type="OMA" id="FHQFVGT"/>
    <dbReference type="OrthoDB" id="110834at2157"/>
    <dbReference type="PhylomeDB" id="Q57851"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-17933"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="EvolutionaryTrace" id="Q57851"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02130">
        <property type="entry name" value="DHNA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027508">
        <property type="entry name" value="DHN_aldolase_MptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036839">
        <property type="entry name" value="MptD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007181">
        <property type="entry name" value="MtpD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04038">
        <property type="entry name" value="DHNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143560">
        <property type="entry name" value="SSF143560"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106857" description="Dihydroneopterin aldolase">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Has 25-fold higher Km towards 7,8-dihydroneopterin than wild-type." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Has 20-fold lower kcat than wild-type." evidence="3">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Has 20-fold lower kcat than wild-type." evidence="3">
        <original>Q</original>
        <variation>A</variation>
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Has 20-fold lower kcat than wild-type." evidence="3">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Has 2-fold lower kcat than wild-type." evidence="3">
        <original>Y</original>
        <variation>F</variation>
        <location>
            <position position="111"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="3"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="7"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="12"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="64"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="83"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="86"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="107"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22931285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="24982305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source ref="4"/>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="22931285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="24982305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7">
        <source ref="4"/>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2OGF"/>
        </source>
    </evidence>
    <sequence length="121" mass="14182" checksum="281669A0B3616F73" modified="1996-11-01" version="1">MRVEETEVFKKYFKNLTDRERAVFEGGITLGALFHQFVGTPVSKYNKESLERAIEEAMKNQPCVYDIKVKIRNVGEKYVSLDGKMLDVDLKIKINKTVAHLKLEYIPEIDYPLMYVKKFEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q57880</accession>
    <name>TRM14_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tRNA (guanine(6)-N2)-methyltransferase</fullName>
            <ecNumber evidence="3">2.1.1.256</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">tRNA m2G6-methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">trm14</name>
        <name type="ordered locus">MJ0438</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="Nucleic Acids Res." volume="39" first="7641" last="7655">
            <title>Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14.</title>
            <authorList>
                <person name="Menezes S."/>
                <person name="Gaston K.W."/>
                <person name="Krivos K.L."/>
                <person name="Apolinario E.E."/>
                <person name="Reich N.O."/>
                <person name="Sowers K.R."/>
                <person name="Limbach P.A."/>
                <person name="Perona J.J."/>
            </authorList>
            <dbReference type="PubMed" id="21693558"/>
            <dbReference type="DOI" id="10.1093/nar/gkr475"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>GENE NAME</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the methylation of the guanosine nucleotide at position 6 (m2G6) in tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>guanosine(6) in tRNA + S-adenosyl-L-methionine = H(+) + N(2)-methylguanosine(6) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:51116"/>
            <dbReference type="Rhea" id="RHEA-COMP:12888"/>
            <dbReference type="Rhea" id="RHEA-COMP:12889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74481"/>
            <dbReference type="EC" id="2.1.1.256"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.256" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64354">
        <property type="entry name" value="F64354"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869937.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57880"/>
    <dbReference type="SMR" id="Q57880"/>
    <dbReference type="STRING" id="243232.MJ_0438"/>
    <dbReference type="PRIDE" id="Q57880"/>
    <dbReference type="EnsemblBacteria" id="AAB98426">
        <property type="protein sequence ID" value="AAB98426"/>
        <property type="gene ID" value="MJ_0438"/>
    </dbReference>
    <dbReference type="GeneID" id="1451298"/>
    <dbReference type="KEGG" id="mja:MJ_0438"/>
    <dbReference type="eggNOG" id="arCOG00048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032119_0_0_2"/>
    <dbReference type="InParanoid" id="Q57880"/>
    <dbReference type="OMA" id="GFMYKAN"/>
    <dbReference type="OrthoDB" id="47556at2157"/>
    <dbReference type="PhylomeDB" id="Q57880"/>
    <dbReference type="BRENDA" id="2.1.1.256">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RNA_methylase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000140480" description="tRNA (guanine(6)-N2)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="43"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="173"/>
            <end position="177"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="204"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="289"/>
            <end position="290"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8U248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="21693558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="21693558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="381" mass="43738" checksum="9BB972D94479444D" modified="1996-11-01" version="1">MDYYVTLSPGLEKISKNEIESFGGKIKEIRENKGRIFFSGDLKLIPKINYLSRTIERMNILLHREEIPNIALDDIYKRVYNIDWTEWIKENQSFAIRPLRAGEHNFTSIDIGRVAGEAVIKSYQRDKNIRLKVNLDEPDVIVRVEVIFDELIVGIDTTGDIALDKRGYRVFNHPAHLNATIASSLVYLSDWKDDEMLLDPMCGSGTIPIEGALMKRNIPPGKFRENKYGFKFIDIFGYELLDKIKKEIVENKNIYKIIGLDKNQKYLDGAKDNAKNAEVLDTIEFICGDATKLHEKFNESDVIIANPPYGIRIGSKRSVKKLYDEFLSSAKEIMHGSSRLIVITAEDKMFKDAIAKNNFEVKEEFNVMFGGLMTRVFYLTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q57903</accession>
    <name>RNP1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribonuclease P protein component 1</fullName>
            <shortName evidence="1">RNase P component 1</shortName>
            <ecNumber evidence="1">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rnp1</name>
        <name type="ordered locus">MJ0464</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2008" name="Nucleic Acids Res." volume="36" first="4172" last="4180">
            <title>Studies on Methanocaldococcus jannaschii RNase P reveal insights into the roles of RNA and protein cofactors in RNase P catalysis.</title>
            <authorList>
                <person name="Pulukkunat D.K."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="18558617"/>
            <dbReference type="DOI" id="10.1093/nar/gkn360"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP4</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2011" name="J. Mol. Biol." volume="411" first="368" last="383">
            <title>Cooperative RNP assembly: complementary rescue of structural defects by protein and RNA subunits of archaeal RNase P.</title>
            <authorList>
                <person name="Chen W.Y."/>
                <person name="Xu Y."/>
                <person name="Cho I.M."/>
                <person name="Oruganti S.V."/>
                <person name="Foster M.P."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="21683084"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2011.05.012"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP4</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2012" name="Nucleic Acids Res." volume="40" first="4666" last="4680">
            <title>Fidelity of tRNA 5'-maturation: a possible basis for the functional dependence of archaeal and eukaryal RNase P on multiple protein cofactors.</title>
            <authorList>
                <person name="Chen W.Y."/>
                <person name="Singh D."/>
                <person name="Lai L.B."/>
                <person name="Stiffler M.A."/>
                <person name="Lai H.D."/>
                <person name="Foster M.P."/>
                <person name="Gopalan V."/>
            </authorList>
            <dbReference type="PubMed" id="22298511"/>
            <dbReference type="DOI" id="10.1093/nar/gks013"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>INTERACTION WITH RNP4</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 3 4">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 3 4">Consists of a catalytic RNA component and at least 4 protein subunits. Forms a subcomplex with Rnp4 which stimulates the catalytic RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64357">
        <property type="entry name" value="H64357"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869964.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6K0A">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.60 A"/>
        <property type="chains" value="E/F=1-95"/>
    </dbReference>
    <dbReference type="PDB" id="6K0B">
        <property type="method" value="EM"/>
        <property type="resolution" value="4.30 A"/>
        <property type="chains" value="E/F=1-95"/>
    </dbReference>
    <dbReference type="PDBsum" id="6K0A"/>
    <dbReference type="PDBsum" id="6K0B"/>
    <dbReference type="AlphaFoldDB" id="Q57903"/>
    <dbReference type="SMR" id="Q57903"/>
    <dbReference type="STRING" id="243232.MJ_0464"/>
    <dbReference type="EnsemblBacteria" id="AAB98453">
        <property type="protein sequence ID" value="AAB98453"/>
        <property type="gene ID" value="MJ_0464"/>
    </dbReference>
    <dbReference type="GeneID" id="1451326"/>
    <dbReference type="KEGG" id="mja:MJ_0464"/>
    <dbReference type="eggNOG" id="arCOG00784">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107020_2_1_2"/>
    <dbReference type="InParanoid" id="Q57903"/>
    <dbReference type="OMA" id="WHELIGL"/>
    <dbReference type="OrthoDB" id="124913at2157"/>
    <dbReference type="PhylomeDB" id="Q57903"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="SSF101744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000128429" description="Ribonuclease P protein component 1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18558617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="21683084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22298511"/>
        </source>
    </evidence>
    <sequence length="95" mass="10888" checksum="8D8DD531C165D09E" modified="1996-11-01" version="1">MITPHNILRHELIGLKVEIVEAKNKAMIGIKGKVVDETRNTLVIEKEDGREVVIPKDIAVFLFQLKGCKVKVDGRLLIGRPEERLKKKIKILYPY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q57935</accession>
    <name>Y515_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0515</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0515</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64364">
        <property type="entry name" value="C64364"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57935"/>
    <dbReference type="SMR" id="Q57935"/>
    <dbReference type="STRING" id="243232.MJ_0515"/>
    <dbReference type="EnsemblBacteria" id="AAB98504">
        <property type="protein sequence ID" value="AAB98504"/>
        <property type="gene ID" value="MJ_0515"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0515"/>
    <dbReference type="eggNOG" id="arCOG01547">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="InParanoid" id="Q57935"/>
    <dbReference type="OMA" id="TFAECIE"/>
    <dbReference type="PhylomeDB" id="Q57935"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014029">
        <property type="entry name" value="NADH_UbQ_OxRdtase_49kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00535">
        <property type="entry name" value="COMPLEX1_49K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000118627" description="Uncharacterized protein MJ0515">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="380" mass="43638" checksum="3EAD16D67834A76F" modified="1996-11-01" version="1">MSENMATIPIGPIHPVLKEPLRIKLVLDGEKPVDAEIEMGYVHRGIEKIMEGKHCHKGIHLAERVCGICSYVHTMTFAECIEHISKIEIPDKAKYLRVVTCELERIHSHLIASAVYNLSIEHETLAMWLLNVREIIMDLMEMITGNRVNMGYNVIGGVRRDINREMMDEIYKKLDIFEDELKNIIEVFETGPLIALRSKEIGILPYHEVMRTRAVGPICRGSGLPESDWRLRHSTYQELKFKPVWREEGDNFARMMVRHEEIIESVRLIREALEHYEECSGDIRVKAEIKGGKGEWRNEAPRGEVTYRMEITDGGIIKRIMIRTPTVMNLEAYKYMLKTCPTVADAVSAYTSIDPCVSCTERCIVAVKDGKEIPISIKFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q57943</accession>
    <name>Y523_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0523</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0523</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64365">
        <property type="entry name" value="C64365"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57943"/>
    <dbReference type="STRING" id="243232.MJ_0523"/>
    <dbReference type="EnsemblBacteria" id="AAB98515">
        <property type="protein sequence ID" value="AAB98515"/>
        <property type="gene ID" value="MJ_0523"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0523"/>
    <dbReference type="eggNOG" id="arCOG04832">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1369520_0_0_2"/>
    <dbReference type="OMA" id="NMILLTD"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019212">
        <property type="entry name" value="DUF2105_membrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011311">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09878">
        <property type="entry name" value="DUF2105"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019136">
        <property type="entry name" value="EhaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106912" description="Uncharacterized protein MJ0523">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="11"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="234" mass="25584" checksum="EBEFD2D776D1B56C" modified="1996-11-01" version="1">MGEMENIIYSIYHPTILVGFAIGILSLLAIGFQKNDLHALILTDVVECAMLIIIAGVGTDLAEALILPGLVVSLAELLAVSEVLITRKYLKSKRPKPKSYKLFEEFKLPLYTGELKYDIHMEILKTSPKFLAIILIVYGAILSGFTGGAVIATGLLFYALSQRVIGVEISEELKTMWEGISGLSGIAWALWIFGFIGFFVFPDKWLLCLLMAGLGLVIKVGSKLGLIGYIGEVR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q57956</accession>
    <name>KORC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2-oxoglutarate synthase subunit KorC</fullName>
            <ecNumber>1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>2-ketoglutarate oxidoreductase gamma chain</fullName>
            <shortName>KOR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>2-oxoglutarate-ferredoxin oxidoreductase subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">korC</name>
        <name type="ordered locus">MJ0536</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2-oxoglutarate + CoA + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64366">
        <property type="entry name" value="H64366"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57956"/>
    <dbReference type="SMR" id="Q57956"/>
    <dbReference type="STRING" id="243232.MJ_0536"/>
    <dbReference type="PRIDE" id="Q57956"/>
    <dbReference type="EnsemblBacteria" id="AAB98530">
        <property type="protein sequence ID" value="AAB98530"/>
        <property type="gene ID" value="MJ_0536"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0536"/>
    <dbReference type="eggNOG" id="arCOG01602">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_0_0_2"/>
    <dbReference type="InParanoid" id="Q57956"/>
    <dbReference type="OMA" id="EVSWMPS"/>
    <dbReference type="PhylomeDB" id="Q57956"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000099946" description="2-oxoglutarate synthase subunit KorC">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <sequence length="187" mass="20548" checksum="4B0687CBDE6B0536" modified="1996-11-01" version="1">MNKRRVKMRKEIRLSGFGGQGIILAGVILGRAAALYDNKEAVQTQSYGPEARGGASKSEVVISDEPIDFPKVIKPDILVCLSQQAYDKYKDDIKEGGVVLVDEDLVSTDKMPEVDVTMYKIPFTRIASEEIKLPIVANIVMLGALTRLTNIVSKESMEKAILDSVPKGTEEKNLLAFSKGYEVAKEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q57960</accession>
    <name>Y540_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0540</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0540</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64367">
        <property type="entry name" value="D64367"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870044.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57960"/>
    <dbReference type="STRING" id="243232.MJ_0540"/>
    <dbReference type="EnsemblBacteria" id="AAB98537">
        <property type="protein sequence ID" value="AAB98537"/>
        <property type="gene ID" value="MJ_0540"/>
    </dbReference>
    <dbReference type="GeneID" id="1451405"/>
    <dbReference type="KEGG" id="mja:MJ_0540"/>
    <dbReference type="eggNOG" id="arCOG05038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_181218_0_0_2"/>
    <dbReference type="OMA" id="YIEVYFG"/>
    <dbReference type="OrthoDB" id="123447at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019208">
        <property type="entry name" value="DUF2097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09870">
        <property type="entry name" value="DUF2097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106921" description="Uncharacterized protein MJ0540">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <sequence length="85" mass="10174" checksum="9978D3AF344582DE" modified="1996-11-01" version="1">MEEIIDVKNPKEVIEYLNNIDVDEYVEIYFGRVHVEGRLMHYNDGLIRLVHEKYGIIEVEIEKILDDLLELVHSNGEKRVVLRFY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q57969</accession>
    <name>NIKR_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0549</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="1">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64368">
        <property type="entry name" value="E64368"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870053.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57969"/>
    <dbReference type="SMR" id="Q57969"/>
    <dbReference type="STRING" id="243232.MJ_0549"/>
    <dbReference type="EnsemblBacteria" id="AAB98541">
        <property type="protein sequence ID" value="AAB98541"/>
        <property type="gene ID" value="MJ_0549"/>
    </dbReference>
    <dbReference type="GeneID" id="8804158"/>
    <dbReference type="KEGG" id="mja:MJ_0549"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_1_2_2"/>
    <dbReference type="InParanoid" id="Q57969"/>
    <dbReference type="OMA" id="AHNCLET"/>
    <dbReference type="OrthoDB" id="90041at2157"/>
    <dbReference type="PhylomeDB" id="Q57969"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0533">Nickel</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000139302" description="Putative nickel-responsive regulator">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <sequence length="141" mass="16133" checksum="5BE0C5F09D38B5ED" modified="1996-11-01" version="1">MTEMDRISISLPSKLLREFDEIIAERGYASRSEAIRDAIRDYIIKHKWIHSLEGERAGSISVIYNHHASDVMEKITEIQHNYTDIIVATLHLHLDHDHCLETILVRGDAKRIRELTDRLTALKGVKQVKLSVMVPGGQIPE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q57994</accession>
    <name>Y574_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0574</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0574</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64371">
        <property type="entry name" value="F64371"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870078.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57994"/>
    <dbReference type="SMR" id="Q57994"/>
    <dbReference type="STRING" id="243232.MJ_0574"/>
    <dbReference type="EnsemblBacteria" id="AAB98574">
        <property type="protein sequence ID" value="AAB98574"/>
        <property type="gene ID" value="MJ_0574"/>
    </dbReference>
    <dbReference type="GeneID" id="1451439"/>
    <dbReference type="KEGG" id="mja:MJ_0574"/>
    <dbReference type="eggNOG" id="arCOG09553">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176001_1_0_2"/>
    <dbReference type="OMA" id="QKRMPDV"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027379">
        <property type="entry name" value="CLS_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13396">
        <property type="entry name" value="PLDc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106937" description="Uncharacterized protein MJ0574">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="93" mass="10446" checksum="60CF4CE6406EB6EF" modified="1996-11-01" version="1">MWPCPIGFGMVGFPIFGFFFMGLFFVIGIAVFIIIIITIVDILKRDALDTLEKILWILVVWFLGIIGAIIYYLLSKRNSKSKGDNNGKNIGSN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58006</accession>
    <name>Y586_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized HTH-type transcriptional regulator MJ0586</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0586</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64373">
        <property type="entry name" value="B64373"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58006"/>
    <dbReference type="SMR" id="Q58006"/>
    <dbReference type="STRING" id="243232.MJ_0586"/>
    <dbReference type="PRIDE" id="Q58006"/>
    <dbReference type="EnsemblBacteria" id="AAB98578">
        <property type="protein sequence ID" value="AAB98578"/>
        <property type="gene ID" value="MJ_0586"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0586"/>
    <dbReference type="eggNOG" id="arCOG01863">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130237_0_0_2"/>
    <dbReference type="InParanoid" id="Q58006"/>
    <dbReference type="OMA" id="VQCEMCG"/>
    <dbReference type="PhylomeDB" id="Q58006"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="CHP00270"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="SSF47413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00270">
        <property type="entry name" value="TIGR00270"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000149791" description="Uncharacterized HTH-type transcriptional regulator MJ0586">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="90"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="H-T-H motif" evidence="1">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00257"/>
        </source>
    </evidence>
    <sequence length="170" mass="19671" checksum="3EC953BDC7FEA6EA" modified="1996-11-01" version="1">MRDSIMQMCELCGKLTDKLYKVIIEGSEMNVCKECAKFGKSPKTYSRLGKKTIIGKGTITTNKQVKKPIKRRRDIFDTLPMLREDYGDVIREAREKRGLSIEELAKKLKMKASTLQKFERYELEPNEKEIKILEKELKISLTESIGEETSYYGGRDEDGFTLGDFIKIKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58023</accession>
    <name>Y606_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0606</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0606</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64375">
        <property type="entry name" value="F64375"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870110.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58023"/>
    <dbReference type="STRING" id="243232.MJ_0606"/>
    <dbReference type="EnsemblBacteria" id="AAB98600">
        <property type="protein sequence ID" value="AAB98600"/>
        <property type="gene ID" value="MJ_0606"/>
    </dbReference>
    <dbReference type="GeneID" id="1451471"/>
    <dbReference type="KEGG" id="mja:MJ_0606"/>
    <dbReference type="eggNOG" id="arCOG05042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183480_0_0_2"/>
    <dbReference type="OMA" id="LTNYVYT"/>
    <dbReference type="OrthoDB" id="109850at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043941">
        <property type="entry name" value="EMC6-arch"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19094">
        <property type="entry name" value="EMC6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106954" description="Uncharacterized protein MJ0606">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="91" mass="9831" checksum="FA2B983CA10406F6" modified="1996-11-01" version="1">MDLEGKCCLIHAIGGIIFGYLANYVYTAGLGIFSGIATLIFLFIGAVIFGHISAKTFGEESLTQKQWLGCGVLPFFLVAIVVWVLKFNGLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q58025</accession>
    <name>PPAC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Manganese-dependent inorganic pyrophosphatase</fullName>
            <ecNumber>3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Pyrophosphate phospho-hydrolase</fullName>
            <shortName>PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">ppaC</name>
        <name type="ordered locus">MJ0608</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Arch. Biochem. Biophys." volume="379" first="292" last="298">
            <title>Methanococcus jannaschii ORF mj0608 codes for a class C inorganic pyrophosphatase protected by Co(2+) or Mn(2+) ions against fluoride inhibition.</title>
            <authorList>
                <person name="Kuhn N.J."/>
                <person name="Wadeson A."/>
                <person name="Ward S."/>
                <person name="Young T.W."/>
            </authorList>
            <dbReference type="PubMed" id="10898947"/>
            <dbReference type="DOI" id="10.1006/abbi.2000.1860"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-5</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>MASS SPECTROMETRY</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>diphosphate + H2O = H(+) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="mass spectrometry" mass="34169.0" method="Electrospray" evidence="2"/>
    <comment type="similarity">
        <text evidence="3">Belongs to the PPase class C family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64375">
        <property type="entry name" value="H64375"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870112.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2EB0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-307"/>
    </dbReference>
    <dbReference type="PDBsum" id="2EB0"/>
    <dbReference type="AlphaFoldDB" id="Q58025"/>
    <dbReference type="SMR" id="Q58025"/>
    <dbReference type="STRING" id="243232.MJ_0608"/>
    <dbReference type="EnsemblBacteria" id="AAB98601">
        <property type="protein sequence ID" value="AAB98601"/>
        <property type="gene ID" value="MJ_0608"/>
    </dbReference>
    <dbReference type="GeneID" id="1451473"/>
    <dbReference type="KEGG" id="mja:MJ_0608"/>
    <dbReference type="eggNOG" id="arCOG01567">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025243_0_1_2"/>
    <dbReference type="InParanoid" id="Q58025"/>
    <dbReference type="OMA" id="IMLCAIL"/>
    <dbReference type="OrthoDB" id="57518at2157"/>
    <dbReference type="PhylomeDB" id="Q58025"/>
    <dbReference type="EvolutionaryTrace" id="Q58025"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00207">
        <property type="entry name" value="PPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022934">
        <property type="entry name" value="Mn-dep_inorganic_PyrPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0170">Cobalt</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000158599" description="Manganese-dependent inorganic pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="2"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="27"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="61"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="100"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="122"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="134"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="189"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="195"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="202"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="247"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="256"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="260"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="269"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="282"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="293"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="298"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10898947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="2EB0"/>
        </source>
    </evidence>
    <sequence length="307" mass="34113" checksum="EF7EDD1610668D9A" modified="1996-11-01" version="1">MRYVVGHKNPDTDSIASAIVLAYFLDCYPARLGDINPETEFVLRKFGVMEPELIESAKGKEIILVDHSEKSQSFDDLEEGKLIAIIDHHKVGLTTTEPILYYAKPVGSTATVIAELYFKDAIDLIGGKKKELKPDLAGLLLSAIISDTVLFKSPTTTDLDKEMAKKLAEIAGISNIEEFGMEILKAKSVVGKLKPEEIINMDFKNFDFNGKKVGIGQVEVIDVSEVESKKEDIYKLLEEKLKNEGYDLIVFLITDIMKEGSEALVVGNKEMFEKAFNVKVEGNSVFLEGVMSRKKQVVPPLERAYNG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58064</accession>
    <name>PFDA2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Prefoldin subunit alpha 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>GimC subunit alpha 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">pfdA2</name>
        <name type="ordered locus">MJ0648</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64380">
        <property type="entry name" value="H64380"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870153.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6VY1">
        <property type="method" value="EM"/>
        <property type="resolution" value="6.00 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/a/b/c/d/e/f/g=1-147"/>
    </dbReference>
    <dbReference type="PDBsum" id="6VY1"/>
    <dbReference type="AlphaFoldDB" id="Q58064"/>
    <dbReference type="SMR" id="Q58064"/>
    <dbReference type="STRING" id="243232.MJ_0648"/>
    <dbReference type="EnsemblBacteria" id="AAB98646">
        <property type="protein sequence ID" value="AAB98646"/>
        <property type="gene ID" value="MJ_0648"/>
    </dbReference>
    <dbReference type="GeneID" id="1451514"/>
    <dbReference type="KEGG" id="mja:MJ_0648"/>
    <dbReference type="eggNOG" id="arCOG01341">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091867_1_3_2"/>
    <dbReference type="InParanoid" id="Q58064"/>
    <dbReference type="OMA" id="RGYMANI"/>
    <dbReference type="OrthoDB" id="106803at2157"/>
    <dbReference type="PhylomeDB" id="Q58064"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12674">
        <property type="entry name" value="PTHR12674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00293">
        <property type="entry name" value="TIGR00293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0143">Chaperone</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000153675" description="Prefoldin subunit alpha 2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="147" mass="16394" checksum="1731EAD91ED0AC9F" modified="1996-11-01" version="1">MVNEVIDINEAVRAYIAQIEGLRAEIGRLDATIATLRQSLATLKSLKTLGEGKTVLVPVGSIAQVEMKVEKMDKVVVSVGQNISAELEYEEALKYIEDEIKKLLTFRLVLEQAIAELYAKIEDLIAEAQQTSEEEKAEEEENEEKAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58071</accession>
    <name>KCY_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Cytidylate kinase</fullName>
            <shortName>CK</shortName>
            <ecNumber>2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cytidine monophosphate kinase</fullName>
            <shortName>CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cmk</name>
        <name type="ordered locus">MJ0656</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + CMP = ADP + CDP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + dCMP = ADP + dCDP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64381">
        <property type="entry name" value="H64381"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870161.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58071"/>
    <dbReference type="SMR" id="Q58071"/>
    <dbReference type="STRING" id="243232.MJ_0656"/>
    <dbReference type="EnsemblBacteria" id="AAB98651">
        <property type="protein sequence ID" value="AAB98651"/>
        <property type="gene ID" value="MJ_0656"/>
    </dbReference>
    <dbReference type="GeneID" id="1451522"/>
    <dbReference type="KEGG" id="mja:MJ_0656"/>
    <dbReference type="eggNOG" id="arCOG01037">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079959_1_0_2"/>
    <dbReference type="InParanoid" id="Q58071"/>
    <dbReference type="OMA" id="ADFRFWL"/>
    <dbReference type="OrthoDB" id="110333at2157"/>
    <dbReference type="PhylomeDB" id="Q58071"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000132013" description="Cytidylate kinase">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="178" mass="20495" checksum="FEC3D475FD8CB7DD" modified="1996-11-01" version="1">MIITIGGLPGTGTTTIAKMIAEKYNLRHVCAGFIFREMAKEMGMDLQEFSKYAEQHKEIDEEIDRRQVEIAKQGNVVLEGRLAAWMLLKNGIKPDLTIWFKAPLEVRAERISKRENIDKDVALKKMIEREASEKKRYKEIYNIDLDDLSIYDLVIDTSKWDVEGVFNIVSSAIDNLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58082</accession>
    <name>Y668_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0668</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0668</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64383">
        <property type="entry name" value="D64383"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58082"/>
    <dbReference type="SMR" id="Q58082"/>
    <dbReference type="STRING" id="243232.MJ_0668"/>
    <dbReference type="EnsemblBacteria" id="AAB98670">
        <property type="protein sequence ID" value="AAB98670"/>
        <property type="gene ID" value="MJ_0668"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0668"/>
    <dbReference type="eggNOG" id="arCOG09634">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200898_0_0_2"/>
    <dbReference type="OMA" id="ILMIRHI"/>
    <dbReference type="PhylomeDB" id="Q58082"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106982" description="Uncharacterized protein MJ0668">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <sequence length="85" mass="10091" checksum="242BB65671A2C305" modified="1996-11-01" version="1">MLFYMLFFLVVIMENDEKIIEDLKIINSKAKFVGIKILMIRHIIESHMKDKKSIYKILESTKNTELYKLILIACPKLEEINEESN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58122</accession>
    <name>G1PDH_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="1">G1P dehydrogenase</shortName>
            <shortName evidence="1">G1PDH</shortName>
            <ecNumber evidence="1">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">egsA</name>
        <name type="ordered locus">MJ0712</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NAD(+) + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + sn-glycerol 1-phosphate = dihydroxyacetone phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64388">
        <property type="entry name" value="H64388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870218.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4RFL">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B/C/D=1-335"/>
    </dbReference>
    <dbReference type="PDB" id="4RGQ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.23 A"/>
        <property type="chains" value="A/B/C/D=1-335"/>
    </dbReference>
    <dbReference type="PDB" id="4RGV">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.45 A"/>
        <property type="chains" value="A/B=1-335"/>
    </dbReference>
    <dbReference type="PDBsum" id="4RFL"/>
    <dbReference type="PDBsum" id="4RGQ"/>
    <dbReference type="PDBsum" id="4RGV"/>
    <dbReference type="AlphaFoldDB" id="Q58122"/>
    <dbReference type="SMR" id="Q58122"/>
    <dbReference type="STRING" id="243232.MJ_0712"/>
    <dbReference type="PRIDE" id="Q58122"/>
    <dbReference type="EnsemblBacteria" id="AAB98707">
        <property type="protein sequence ID" value="AAB98707"/>
        <property type="gene ID" value="MJ_0712"/>
    </dbReference>
    <dbReference type="GeneID" id="1451590"/>
    <dbReference type="KEGG" id="mja:MJ_0712"/>
    <dbReference type="eggNOG" id="arCOG00982">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038362_0_0_2"/>
    <dbReference type="InParanoid" id="Q58122"/>
    <dbReference type="OMA" id="ALHGEQC"/>
    <dbReference type="OrthoDB" id="57545at2157"/>
    <dbReference type="PhylomeDB" id="Q58122"/>
    <dbReference type="BRENDA" id="1.1.1.261">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NAD+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase [NADP+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="PTHR43616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0444">Lipid biosynthesis</keyword>
    <keyword id="KW-0443">Lipid metabolism</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword id="KW-1208">Phospholipid metabolism</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000157342" description="Glycerol-1-phosphate dehydrogenase [NAD(P)+]">
        <location>
            <begin position="1"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="78"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="100"/>
            <end position="103"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="19"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="31"/>
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        </location>
    </feature>
    <feature type="turn" evidence="2">
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            <begin position="37"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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        </location>
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    <feature type="helix" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="105"/>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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            <end position="112"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
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        </location>
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    <feature type="helix" evidence="2">
        <location>
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        </location>
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    <feature type="helix" evidence="2">
        <location>
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    <feature type="helix" evidence="2">
        <location>
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    <feature type="helix" evidence="2">
        <location>
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    <feature type="helix" evidence="2">
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    <feature type="helix" evidence="2">
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    <feature type="helix" evidence="2">
        <location>
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    <feature type="helix" evidence="2">
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    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="322"/>
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="4RFL"/>
        </source>
    </evidence>
    <sequence length="335" mass="37275" checksum="A99C777C3126367B" modified="1996-11-01" version="1">MIIVTPRYTIIEDGAINKIEEILKKLNLKNPLVITGKNTKKYCRFFYDIVYYDEILNNLEIELKKYTAYDCVIGIGGGRSIDTGKYLAYKLGIPFISVPTTASNDGIASPIVSIRQPSFMVDAPIAIIADTEIIKKSPRRLLSAGMGDIVSNITAVLDWKLAYKEKGEKYSESSAIFSKTIAKELISYVLNSDLSEYHNKLVKALVGSGIAIAIANSSRPASGSEHLFSHALDKLKEEYNLNINSLHGEQCGIGTIMMSYLHEKENKKLSGLHEKIKMSLKKVDAPTTAKELGFDEDIIIEALTMAHKIRNRWTILRDGLSREEARKLAEETGVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="71" xmlns="http://uniprot.org/uniprot">
    <accession>Q58168</accession>
    <name>Y758_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0758</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0758</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64394">
        <property type="entry name" value="F64394"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58168"/>
    <dbReference type="STRING" id="243232.MJ_0758"/>
    <dbReference type="EnsemblBacteria" id="AAB98758">
        <property type="protein sequence ID" value="AAB98758"/>
        <property type="gene ID" value="MJ_0758"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0758"/>
    <dbReference type="eggNOG" id="arCOG08262">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_145180_0_0_2"/>
    <dbReference type="OMA" id="CPFMYLL"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107017" description="Uncharacterized protein MJ0758">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <sequence length="159" mass="18245" checksum="99ABB8A49BBD2F56" modified="1996-11-01" version="1">MVCSRGIFGIDIMNKKGIDSLFVSALYYSINKAIYDVMGDGGKVLGRRASYEMIKLLKDLGFIKENMSNEEIKNLFVNTFGLSEDLNIVEEDKKVIFEVINPTLDLFLKKLMEENLKPYVCPFMYLLSEIYSVSNNCRLMLSDVVPETEEKVKLIFKKV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q58191</accession>
    <name>Y781_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0781</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Mja klbA intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ordered locus">MJ0781</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="2">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64397">
        <property type="entry name" value="E64397"/>
    </dbReference>
    <dbReference type="PDB" id="2JMZ">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=398-577"/>
    </dbReference>
    <dbReference type="PDB" id="2JNQ">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=398-577"/>
    </dbReference>
    <dbReference type="PDBsum" id="2JMZ"/>
    <dbReference type="PDBsum" id="2JNQ"/>
    <dbReference type="AlphaFoldDB" id="Q58191"/>
    <dbReference type="BMRB" id="Q58191"/>
    <dbReference type="SMR" id="Q58191"/>
    <dbReference type="STRING" id="243232.MJ_0781"/>
    <dbReference type="EnsemblBacteria" id="AAB98770">
        <property type="protein sequence ID" value="AAB98770"/>
        <property type="gene ID" value="MJ_0781"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0781"/>
    <dbReference type="eggNOG" id="arCOG01819">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_0_1_2"/>
    <dbReference type="InParanoid" id="Q58191"/>
    <dbReference type="OMA" id="TGHDGCS"/>
    <dbReference type="PhylomeDB" id="Q58191"/>
    <dbReference type="EvolutionaryTrace" id="Q58191"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000013122" description="Uncharacterized protein MJ0781, 1st part" evidence="1">
        <location>
            <begin position="1"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013123" description="Mja klbA intein" evidence="1">
        <location>
            <begin position="405"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013124" description="Uncharacterized protein MJ0781, 2nd part" evidence="1">
        <location>
            <begin position="573"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="308"/>
            <end position="315"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="434"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="446"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="450"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="455"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="461"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="468"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="473"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="481"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="501"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="509"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="515"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="522"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="529"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="542"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="549"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="555"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="558"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="568"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2JMZ"/>
        </source>
    </evidence>
    <sequence length="721" mass="82743" checksum="13B76CEC7976F0F3" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58244</accession>
    <name>Y834_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0834</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0834</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64404">
        <property type="entry name" value="B64404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870346.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58244"/>
    <dbReference type="STRING" id="243232.MJ_0834"/>
    <dbReference type="EnsemblBacteria" id="AAB98837">
        <property type="protein sequence ID" value="AAB98837"/>
        <property type="gene ID" value="MJ_0834"/>
    </dbReference>
    <dbReference type="GeneID" id="1451718"/>
    <dbReference type="KEGG" id="mja:MJ_0834"/>
    <dbReference type="eggNOG" id="arCOG09555">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1253590_0_0_2"/>
    <dbReference type="OMA" id="SCQLEWI"/>
    <dbReference type="OrthoDB" id="95135at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="SSF54001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107069" description="Uncharacterized protein MJ0834">
        <location>
            <begin position="1"/>
            <end position="238"/>
        </location>
    </feature>
    <sequence length="238" mass="28773" checksum="15795760AC988941" modified="1996-11-01" version="1">MFMDYKYFFITIILISIFCGCYEKSYSFVEYNRHYELNEPNNTKNPNYDQNIFLNHDLPKTYPKMYKFPKNYYELSDKMFPDVKKRDLDTLSYILKTIKLPAYKKNYYDCSEASCQLEWILEGYGFKTYLVYGILDTYGNSGSHMWVAVQLDNGKMVLVESTYLCENYYCPDYAIIYKNYNLNNIVIVRDMKYIPKFYADTPDMFLIPHNNRRFLITQLDWWNHPKNAEIKKEMFNLK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58275</accession>
    <name>Y865_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative methylthiotransferase MJ0865</fullName>
            <ecNumber>2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0865</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methylthiotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64408">
        <property type="entry name" value="A64408"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58275"/>
    <dbReference type="SMR" id="Q58275"/>
    <dbReference type="STRING" id="243232.MJ_0865"/>
    <dbReference type="EnsemblBacteria" id="AAB98870">
        <property type="protein sequence ID" value="AAB98870"/>
        <property type="gene ID" value="MJ_0865"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0865"/>
    <dbReference type="eggNOG" id="arCOG01356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_600805_0_0_2"/>
    <dbReference type="InParanoid" id="Q58275"/>
    <dbReference type="OMA" id="THRGCLG"/>
    <dbReference type="PhylomeDB" id="Q58275"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023979">
        <property type="entry name" value="CHP04014_B12-bd/rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01217">
        <property type="entry name" value="B12-binding_methylthiotransfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04014">
        <property type="entry name" value="B12_SAM_MJ_0865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000141759" description="Putative methylthiotransferase MJ0865">
        <location>
            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="3">
        <location>
            <begin position="163"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="449" mass="50957" checksum="6A97907DAF81DBB6" modified="1996-11-01" version="1">MNKIKEGANVRITIYSPEVYTYGAMLIGGILKHKGYNVHLVRKIDKTLFLKSDVIIFSLYSTLHILDKNIREAIDFIKKVRKNKTKVYVAGCVSTYPEIILNELNVDGVIVGEGEITTPKIIEGDKEGLAYKEGDEIVINYPKEKPDLNHPLPLIPKDIEQQSIRGANVYIETHRGCLGNCTFCQVPKFFGKTIRSRDVEDVVEEVKAFKRAGAKRIAISGGTGSLYAFKKSINRDKFFELLEKVSEVIGKNNLSVPDMRVDYVDEEILEAIKNYTIGWVFYGIESGSDKILKDMKKGTNREKNLDAIKLAKDCGVKVAGSFIVAYPTETEMDYLLTKDFIVDAELDDVFVSIAEPIPTTELCDLVLSMPKEENLLYKMHEGEYRKLGLSEAEARCFDLLIHAEMWKSMPKPLTPQLYNLYLNEARIQGKDIRAITDLLFKYRDLLLKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58282</accession>
    <name>Y872_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized lipoprotein MJ0872</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0872</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98880" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98880.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64408">
        <property type="entry name" value="H64408"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496645.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58282"/>
    <dbReference type="SMR" id="Q58282"/>
    <dbReference type="STRING" id="243232.MJ_0872"/>
    <dbReference type="PRIDE" id="Q58282"/>
    <dbReference type="EnsemblBacteria" id="AAB98880">
        <property type="protein sequence ID" value="AAB98880"/>
        <property type="gene ID" value="MJ_0872"/>
    </dbReference>
    <dbReference type="GeneID" id="1451761"/>
    <dbReference type="KEGG" id="mja:MJ_0872"/>
    <dbReference type="eggNOG" id="arCOG03417">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025776_0_0_2"/>
    <dbReference type="InParanoid" id="Q58282"/>
    <dbReference type="OMA" id="NWYAVGG"/>
    <dbReference type="OrthoDB" id="90656at2157"/>
    <dbReference type="PhylomeDB" id="Q58282"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014004" description="Uncharacterized lipoprotein MJ0872">
        <location>
            <begin position="21"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="3">
        <location>
            <begin position="104"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-archaeol cysteine" evidence="1">
        <location>
            <position position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="391" mass="44910" checksum="C063343A4AF51CA4" modified="2004-05-10" version="2" precursor="true">MRKLFLLSILMIGVIVAFAGCVEESKTTTQLQQTTQSESQKAETQPKLGVNVVRYAETFKLYPHWDEGYCVVADSVGNKFVLVEGNAKAPNISDGKIIKVPVKRIVTDFYCPIISAADILNAYHHTIVGAPKYAVEKSPKLKELFDEGKVVDIGSPSKGVNYELIVNLTPDIVFLGDWKSEDVVEEKLKELGVTVSRFYTYQEPTYMGRVEWIKFAAAFWGSNAYKKADKWFENVVKVRENILKKVQNVTNEPTVVIFSWSKTKNMPGIYGNDSYYSKMIAEFKGKNVFDDYNRGYQYVDKETFYERAMNADVVILIWFYGDVKTKEDLLKINPNFAEFKAFKTGRFYVSHPDYYVWEARDPAGYMMDFAKMIHPELFGGDDDLKYYYKIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58290</accession>
    <name>Y880_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0880</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0880</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0871, MJ1556 and MJ1589.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64409">
        <property type="entry name" value="H64409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870394.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58290"/>
    <dbReference type="STRING" id="243232.MJ_0880"/>
    <dbReference type="TCDB" id="9.B.156.2.2">
        <property type="family name" value="the putative 4-10 tms permease (4-10p) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98884">
        <property type="protein sequence ID" value="AAB98884"/>
        <property type="gene ID" value="MJ_0880"/>
    </dbReference>
    <dbReference type="GeneID" id="1451769"/>
    <dbReference type="KEGG" id="mja:MJ_0880"/>
    <dbReference type="eggNOG" id="arCOG00360">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048086_2_0_2"/>
    <dbReference type="InParanoid" id="Q58290"/>
    <dbReference type="OMA" id="HQFPYYA"/>
    <dbReference type="OrthoDB" id="59004at2157"/>
    <dbReference type="PhylomeDB" id="Q58290"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="PTHR38139"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107089" description="Uncharacterized protein MJ0880">
        <location>
            <begin position="1"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="178"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="308" mass="34204" checksum="CF8BBA7524C83530" modified="1996-11-01" version="1">MDILPYLTKIILLSSIGITIASIIVETNLISKIKKITKPICLISNLPEECVVSLLGNFINPTVGKSMLSGFYKENKVNEKEVIVTTIISPLPTILGESVFRVQLPLAVVILGYKLGLIYVSLNVISGFLQALIGILYANIFFERRQINIDNNNNEKIVFNREVIIKGFKKSLKILKKVIPMIVIFTLLINFLIKLGLMDVVKGLFSPIFRILDLPGEAITVLIANLAHFSAGYTTVDILIKNGVLNEKQALIVLLIGNIISVTMIYLKHSIGTYISLFGRFGLKLAVINYTISVMIKILLILLLIAFF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58304</accession>
    <name>FLAC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative flagella-related protein C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaC</name>
        <name type="ordered locus">MJ0894</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">To M.voltae FlaC.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64411">
        <property type="entry name" value="F64411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870408.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58304"/>
    <dbReference type="SMR" id="Q58304"/>
    <dbReference type="STRING" id="243232.MJ_0894"/>
    <dbReference type="EnsemblBacteria" id="AAB98897">
        <property type="protein sequence ID" value="AAB98897"/>
        <property type="gene ID" value="MJ_0894"/>
    </dbReference>
    <dbReference type="GeneID" id="1451783"/>
    <dbReference type="KEGG" id="mja:MJ_0894"/>
    <dbReference type="eggNOG" id="arCOG05119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123708_0_0_2"/>
    <dbReference type="InParanoid" id="Q58304"/>
    <dbReference type="OMA" id="NQINPFI"/>
    <dbReference type="OrthoDB" id="87816at2157"/>
    <dbReference type="PhylomeDB" id="Q58304"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009205">
        <property type="entry name" value="FlaC_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05377">
        <property type="entry name" value="FlaC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018660">
        <property type="entry name" value="Archl_flaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000087267" description="Putative flagella-related protein C">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="141" mass="16244" checksum="A634B2A299355FA2" modified="1996-11-01" version="1">METTEGLLAKVNDIESKLPKLESSINNLRKENEMLRVELNKINENLQDIMALYEVVSNQINPFIGVSKITATSLEKLERLETEYKRLKKTVEELTNDLIILGSLYLHQLDINLDEIIEEVLEEEIIKSMSGEDTHDTKDNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58305</accession>
    <name>FLAD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative flagella-related protein D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaD</name>
        <name type="ordered locus">MJ0895</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">To M.voltae FlaD, also to FlaE.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64411">
        <property type="entry name" value="G64411"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870409.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58305"/>
    <dbReference type="SMR" id="Q58305"/>
    <dbReference type="STRING" id="243232.MJ_0895"/>
    <dbReference type="EnsemblBacteria" id="AAB98898">
        <property type="protein sequence ID" value="AAB98898"/>
        <property type="gene ID" value="MJ_0895"/>
    </dbReference>
    <dbReference type="GeneID" id="1451784"/>
    <dbReference type="KEGG" id="mja:MJ_0895"/>
    <dbReference type="eggNOG" id="arCOG02964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047519_0_0_2"/>
    <dbReference type="InParanoid" id="Q58305"/>
    <dbReference type="OMA" id="YYEIGWI"/>
    <dbReference type="OrthoDB" id="61570at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006752">
        <property type="entry name" value="Arch_fla_DE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016682">
        <property type="entry name" value="FlaD_prd_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04659">
        <property type="entry name" value="Arch_fla_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017066">
        <property type="entry name" value="FlaD_arch_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000087269" description="Putative flagella-related protein D">
        <location>
            <begin position="1"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="342" mass="39951" checksum="B384DDDE1775566C" modified="1998-12-15" version="2">MIQKTISETSPPPMFSDEEILTEDEIEEYLDNLKSKLPSFVIILLKNNLRGKRVTKKQLDKIVERITEVLSKGRRDDKTEELNKKLQTLEQKLDAIMKLTTMAVSTKTSEEIEKIKTNEKIEIENAPIKIKSEEKPEKPVEIEVSKKKETMDTGKKTETEEKVKEIEVPKPVEKTHEKVEETKTKGEIKKEVKKEVKLKKYELPKESPMGGSFMTPIEEEKEYRLNDIPEDAVSMTLVFKWLEFLISRGGMTYLPDILDYYNKIGWISNRVILKLLRFAKNMKITFDEEELRPRDKLSPSDHIVSLLYIEKLAGRPIDSEILEMLEIEIRRIKKWAIELQSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58306</accession>
    <name>FLAE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative flagella-related protein E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaE</name>
        <name type="ordered locus">MJ0896</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">To M.voltae FlaE, also to FlaD.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64411">
        <property type="entry name" value="H64411"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58306"/>
    <dbReference type="SMR" id="Q58306"/>
    <dbReference type="STRING" id="243232.MJ_0896"/>
    <dbReference type="EnsemblBacteria" id="AAB98899">
        <property type="protein sequence ID" value="AAB98899"/>
        <property type="gene ID" value="MJ_0896"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0896"/>
    <dbReference type="eggNOG" id="arCOG02964">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881106_0_0_2"/>
    <dbReference type="InParanoid" id="Q58306"/>
    <dbReference type="OMA" id="LEYLCER"/>
    <dbReference type="PhylomeDB" id="Q58306"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006752">
        <property type="entry name" value="Arch_fla_DE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04659">
        <property type="entry name" value="Arch_fla_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000087271" description="Putative flagella-related protein E">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="140" mass="15892" checksum="2CF9E580E9D09146" modified="1996-11-01" version="1">MGEVFKMDGLASTILEVHKPAKLEDIPDEDPIAIILALKWLEYLCERAGVENVSDILDFYYMLGWLGDKALAKLLKFLKGIKVDEENVVEGSGKLNITDHIISLLFIERLNGKKISAELLDKIEWELRKIKKGAEQFYGI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58307</accession>
    <name>FLAF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative flagella-related protein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaF</name>
        <name type="ordered locus">MJ0897</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Archaeal flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.voltae FlaF.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64412">
        <property type="entry name" value="A64412"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870411.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58307"/>
    <dbReference type="STRING" id="243232.MJ_0897"/>
    <dbReference type="EnsemblBacteria" id="AAB98900">
        <property type="protein sequence ID" value="AAB98900"/>
        <property type="gene ID" value="MJ_0897"/>
    </dbReference>
    <dbReference type="GeneID" id="1451786"/>
    <dbReference type="KEGG" id="mja:MJ_0897"/>
    <dbReference type="eggNOG" id="arCOG01824">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881047_0_0_2"/>
    <dbReference type="OMA" id="VSEYGNQ"/>
    <dbReference type="OrthoDB" id="101546at2157"/>
    <dbReference type="PhylomeDB" id="Q58307"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0007">Acetylation</keyword>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-0449">Lipoprotein</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000021270" description="Putative flagella-related protein F">
        <location>
            <begin position="19"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylcysteine" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-archaeol cysteine" evidence="1">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="139" mass="15624" checksum="4FB4BB7170E40C06" modified="1996-11-01" version="1" precursor="true">MGFSSVVGATVMIIALLVCGAYLYVTMDSYYENVDEAYTTYYSHVHAKLNEKLVITDVKSSTSQTNITIYNNGSVVVEPDKFTILFDGTVVPEENISYYPKLKKYLVPLDSITIVVNWTQPSRICIVSDNGNKYFYSLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58310</accession>
    <name>Y900_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0900</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0900</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64412">
        <property type="entry name" value="D64412"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58310"/>
    <dbReference type="SMR" id="Q58310"/>
    <dbReference type="STRING" id="243232.MJ_0900"/>
    <dbReference type="EnsemblBacteria" id="AAB98903">
        <property type="protein sequence ID" value="AAB98903"/>
        <property type="gene ID" value="MJ_0900"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0900"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005379_2_2_2"/>
    <dbReference type="InParanoid" id="Q58310"/>
    <dbReference type="OMA" id="CLEYGMS"/>
    <dbReference type="PhylomeDB" id="Q58310"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000207310" description="Uncharacterized protein MJ0900">
        <location>
            <begin position="1"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="551" mass="63493" checksum="2BFC8A31CA32A1BC" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58315</accession>
    <name>Y905_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0905</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0905</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64413">
        <property type="entry name" value="A64413"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870419.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58315"/>
    <dbReference type="SMR" id="Q58315"/>
    <dbReference type="STRING" id="243232.MJ_0905"/>
    <dbReference type="EnsemblBacteria" id="AAB98915">
        <property type="protein sequence ID" value="AAB98915"/>
        <property type="gene ID" value="MJ_0905"/>
    </dbReference>
    <dbReference type="GeneID" id="1451794"/>
    <dbReference type="KEGG" id="mja:MJ_0905"/>
    <dbReference type="eggNOG" id="arCOG03422">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_174518_0_0_2"/>
    <dbReference type="InParanoid" id="Q58315"/>
    <dbReference type="OMA" id="YYRLTEY"/>
    <dbReference type="OrthoDB" id="108207at2157"/>
    <dbReference type="PhylomeDB" id="Q58315"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010863">
        <property type="entry name" value="DUF1495"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07381">
        <property type="entry name" value="DUF1495"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107097" description="Uncharacterized protein MJ0905">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <sequence length="111" mass="12873" checksum="AF34D2852F85715F" modified="1996-11-01" version="1">MSLAFIDPMIIRSLNKSKLRKKILYLLYKMYPHGIYLSEISRRVRSDPSNVLGCLKGMNGRYNGHFSLIELGLVECVERGGVKIYKLTDYGKKIVEVLKDQDSDFIESLRW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58320</accession>
    <name>Y910_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0910</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0910</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="F64413"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58320"/>
    <dbReference type="SMR" id="Q58320"/>
    <dbReference type="STRING" id="243232.MJ_0910"/>
    <dbReference type="EnsemblBacteria" id="AAB98912">
        <property type="protein sequence ID" value="AAB98912"/>
        <property type="gene ID" value="MJ_0910"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0910"/>
    <dbReference type="eggNOG" id="arCOG06472">
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    <dbReference type="HOGENOM" id="CLU_016684_2_1_2"/>
    <dbReference type="OMA" id="IMEHERA"/>
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        <property type="component" value="Chromosome"/>
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    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF53300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107100" description="Uncharacterized protein MJ0910">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <sequence length="174" mass="19707" checksum="A28670E3FF18042A" modified="1996-11-01" version="1">MGAMRRMEAAKGAIISLLLDAYQKRNKIGMIAFRKDKAELILPFTSSVELGEKLLKDLPTGGKTPLADAFIKSYEVFDREIRKNPNIIPIMIVISDFKPNVAVKEDYVKEVFDACEKIAEKGINVILIDTEPQSFIKIGIGKEIANRFGFKYYKIEELSKDKILDICKSLEINF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-08-03" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>Q58344</accession>
    <name>Y934_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized polyferredoxin-like protein MJ0934</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0934</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="243232.MJ_0934"/>
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    </dbReference>
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    <dbReference type="eggNOG" id="arCOG02184">
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    <dbReference type="HOGENOM" id="CLU_1521877_0_0_2"/>
    <dbReference type="InParanoid" id="Q58344"/>
    <dbReference type="OMA" id="NCKLCIQ"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
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        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
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        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="5"/>
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        <property type="match status" value="6"/>
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    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
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            <end position="209"/>
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            <begin position="38"/>
            <end position="67"/>
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            <begin position="63"/>
            <end position="92"/>
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            <begin position="90"/>
            <end position="119"/>
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            <begin position="122"/>
            <end position="151"/>
        </location>
    </feature>
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        <location>
            <begin position="145"/>
            <end position="174"/>
        </location>
    </feature>
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        <location>
            <begin position="179"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
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        </ligand>
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        </location>
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            <dbReference type="ChEBI" id="CHEBI:49883"/>
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            <name>[4Fe-4S] cluster</name>
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    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="209" mass="23567" checksum="755EDCFF8077D54B" modified="1996-11-01" version="1">MRFMEEEFYKIFKGAGLIKTLIRSIFLTNRNKFSKPSKTKPIQLTECIGCGLCVDVCPTNAIKIFSFRETICSVCGTCVDVCPNNAIIKDRFTIDADKCTKCGVCVLFCPIPIIKKEIPKPKTPVILKDRCNSCGLCECEAIDIINKEINPEKCKLCLSCIEKCPLQAILTPDEYINSLIVKVDIDSCIFCRECEEICPIRGNYEHRES</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58353</accession>
    <name>Y943_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0943</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0943</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="G64417"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58353"/>
    <dbReference type="EnsemblBacteria" id="AAB98953">
        <property type="protein sequence ID" value="AAB98953"/>
        <property type="gene ID" value="MJ_0943"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0943"/>
    <dbReference type="eggNOG" id="arCOG03788">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_658268_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107114" description="Uncharacterized protein MJ0943">
        <location>
            <begin position="1"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="10"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="148"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="417" mass="46355" checksum="6DF8D82E1AA8BD90" modified="1996-11-01" version="1">MITMDTIRKALVCFSILSILVLACGCVNTPEKIDININSNTNNGENTEKPINQENQNVNNVENKKESQSTQNIQSYENKEIKNQENHPLQSNQNYEQTNGNFNEENENAMTNVGESEVNYNNEPAYNYYIEITYPDGTIPDKIEEQMLYYIKVIDPIVGGLAGIDIYVDGNYIGTLDDVYGIVECVFYEPGYHTITAEDNGKILASKTVYVEEGTAYNSGESENYDEYDNNYESNDLQQTQTQFSEIEVYVDDIKPSNSIIITKLAMNPGFLASINGISPDIGVNIEMENGEKINLKYVSMDVDLIIDNPNSESITIDKIILNMFDDEGHSLGRGEVSNIVITPGENPVTVKVNIPINKMGYEILRKLSGEEVFAEISGSAYIEGSGEVPFSGEADLLPPLPTPPFPLPPLPPFPTE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58379</accession>
    <name>POK_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantoate kinase</fullName>
            <shortName evidence="1">PoK</shortName>
            <ecNumber evidence="1">2.7.1.169</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0969</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64421">
        <property type="entry name" value="A64421"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870483.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58379"/>
    <dbReference type="SMR" id="Q58379"/>
    <dbReference type="STRING" id="243232.MJ_0969"/>
    <dbReference type="EnsemblBacteria" id="AAB98974">
        <property type="protein sequence ID" value="AAB98974"/>
        <property type="gene ID" value="MJ_0969"/>
    </dbReference>
    <dbReference type="GeneID" id="1451867"/>
    <dbReference type="KEGG" id="mja:MJ_0969"/>
    <dbReference type="eggNOG" id="arCOG04263">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081191_0_0_2"/>
    <dbReference type="InParanoid" id="Q58379"/>
    <dbReference type="OMA" id="MLGETVF"/>
    <dbReference type="OrthoDB" id="104702at2157"/>
    <dbReference type="PhylomeDB" id="Q58379"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PTHR42282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107123" description="Pantoate kinase">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <sequence length="270" mass="30035" checksum="F594A63020D4E66B" modified="1996-11-01" version="1">MFAPGHITGFFVICKSSNKLKTGSIGAGITIDRGVNVELKEGNGSIFYNNKKVNICAVEKVIEHYKKFGYNDDYDIIFSSDFPLGSGLGMSGGCALILAKKLNEMLNLNENYAEIAHISEVECGTGLGDVIAQYVKGFVIRKTPGFPINVEKIVVDDDYYIIIEIFGKKETKEIITNDIWIKKINEYGERCLNELLKNPTLENFVNLSYEFAVNTGLINEKILSICEDLKFTVGASQSMLGNTLFCISKKETLEDALSILKNPIVCNIYY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-16" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58433</accession>
    <name>Y1027_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1027</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1027</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the complex I 49 kDa subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64428">
        <property type="entry name" value="B64428"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58433"/>
    <dbReference type="SMR" id="Q58433"/>
    <dbReference type="STRING" id="243232.MJ_1027"/>
    <dbReference type="EnsemblBacteria" id="AAB99031">
        <property type="protein sequence ID" value="AAB99031"/>
        <property type="gene ID" value="MJ_1027"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1027"/>
    <dbReference type="eggNOG" id="arCOG01547">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015134_1_2_2"/>
    <dbReference type="InParanoid" id="Q58433"/>
    <dbReference type="OMA" id="CDPCFTC"/>
    <dbReference type="PhylomeDB" id="Q58433"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="SSF56762"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000118628" description="Uncharacterized protein MJ1027">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="377" mass="43175" checksum="913C3C1A6BE0332C" modified="1996-11-01" version="1">MLFMKYEGEIAIGPVHPTMLEPHRLRLFIEDEIIKEAELVIGVNYRGIELIMEGLPPEKISILSEKICGICSHIHVWCNVTVTERGCDIEVPERAEYIRAIVEELERLHSHMLLFGHAFEVLGFETMAFRAFMIREPIMQILGEITGGRAQYSCPIIGGIRPRCNIKESKIPALLERLEKFEENLKKLLERTVNDPMIMSRIKDVGVLDKKTAKKLHALGPTARGSGIHSDMRKMGQVPVYDNFEFEEIVFDDGDVFSRLAVRFYECFESVKIIRQGLKDLPNLDKRIYNPNYELKEFKPIDVYNEAQRGQVYYSYGLDENGRVRQVKIRTPTATNLACMEAILPGHHVSDAELIIASCDPCFTCTDRMIVVKENKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58460</accession>
    <name>Y1060_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1060</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1060</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64432">
        <property type="entry name" value="C64432"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870573.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58460"/>
    <dbReference type="STRING" id="243232.MJ_1060"/>
    <dbReference type="EnsemblBacteria" id="AAB99072">
        <property type="protein sequence ID" value="AAB99072"/>
        <property type="gene ID" value="MJ_1060"/>
    </dbReference>
    <dbReference type="GeneID" id="1451957"/>
    <dbReference type="KEGG" id="mja:MJ_1060"/>
    <dbReference type="eggNOG" id="arCOG06580">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_506818_0_0_2"/>
    <dbReference type="OMA" id="NMILAIT"/>
    <dbReference type="OrthoDB" id="38791at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107154" description="Uncharacterized protein MJ1060">
        <location>
            <begin position="1"/>
            <end position="537"/>
        </location>
    </feature>
    <sequence length="537" mass="65990" checksum="83E8A0C63B6D0837" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58470</accession>
    <name>Y1070_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1070</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1070</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64433">
        <property type="entry name" value="E64433"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58470"/>
    <dbReference type="SMR" id="Q58470"/>
    <dbReference type="STRING" id="243232.MJ_1070"/>
    <dbReference type="EnsemblBacteria" id="AAB99077">
        <property type="protein sequence ID" value="AAB99077"/>
        <property type="gene ID" value="MJ_1070"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1070"/>
    <dbReference type="eggNOG" id="arCOG05067">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_135436_0_0_2"/>
    <dbReference type="OMA" id="THYGNIG"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014007" description="Uncharacterized protein MJ1070">
        <location>
            <begin position="20"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="157" mass="18240" checksum="75CD5561820D9429" modified="1996-11-01" version="1" precursor="true">MRKYLIILVLLLFLSSSFGYYFDYIKVSESNPIKTITFKINKAENYSYKLSFVHYGNINKSMKVNIYLNGNLAYTIDDSNDASPAYKKNASIDITNYLKDGENVLKVEGMNLIGNENYHPYYVLKDIYINEPAKTPIDFKLMIYALLIICFLIYKKC</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q58496</accession>
    <name>ARGC_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="1">AGPR</shortName>
            <ecNumber evidence="1">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="1">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argC</name>
        <name type="ordered locus">MJ1096</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = H(+) + N-acetyl-L-glutamyl 5-phosphate + NADPH</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99099" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99099.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64436">
        <property type="entry name" value="G64436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870608.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58496"/>
    <dbReference type="SMR" id="Q58496"/>
    <dbReference type="STRING" id="243232.MJ_1096"/>
    <dbReference type="EnsemblBacteria" id="AAB99099">
        <property type="protein sequence ID" value="AAB99099"/>
        <property type="gene ID" value="MJ_1096"/>
    </dbReference>
    <dbReference type="GeneID" id="1451992"/>
    <dbReference type="KEGG" id="mja:MJ_1096"/>
    <dbReference type="eggNOG" id="arCOG00495">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006384_0_1_2"/>
    <dbReference type="InParanoid" id="Q58496"/>
    <dbReference type="OMA" id="PHLTPMI"/>
    <dbReference type="OrthoDB" id="51061at2157"/>
    <dbReference type="PhylomeDB" id="Q58496"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000112486" description="N-acetyl-gamma-glutamyl-phosphate reductase">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="341" mass="38172" checksum="72AE13AD35061D88" modified="2000-05-30" version="2">MKEVAIIGATGYTGAELLRLLANHEKVNVTYITSRKEAGKHVFKVHPHLKGIEKYKNLCFTGDIDKVDAYLVFTATPHGASMDIVPDFIERGMKVIDLSGDYRFEDLSLYEKYYKIKHKGLPDVKIAYGLPELHREEIKEAQLVANPGCFPTGAILAVAPLVKENIIEERIIFDSKTGVSGAGIKPTETTHFPNVNENINPYKITTHRHTPEIEKELKKLGKAKVSFTPHLAPITRGILTTAHTFLAKDVDREEIIKIYEKFYGSEVFVRIFSEEIPKLTWVRGTNFCDIGGFEIDEHGRLVVISAIDNLVKGASGQAIQNMNIMFGFDEKEGLFDVGLNP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58499</accession>
    <name>MFNB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">(5-formylfuran-3-yl)methyl phosphate synthase</fullName>
            <ecNumber evidence="1 4">4.2.3.153</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 6">4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase</fullName>
            <shortName evidence="1 5">4-HFC-P synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 5" type="primary">mfnB</name>
        <name type="ordered locus">MJ1099</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="Biochemistry" volume="53" first="4635" last="4647">
            <title>Biosynthesis of the 5-(aminomethyl)-3-furanmethanol moiety of methanofuran.</title>
            <authorList>
                <person name="Miller D."/>
                <person name="Wang Y."/>
                <person name="Xu H."/>
                <person name="Harich K."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="24977328"/>
            <dbReference type="DOI" id="10.1021/bi500615p"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>PATHWAY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2015" name="Biochemistry" volume="54" first="2997" last="3008">
            <title>Mechanism of the enzymatic synthesis of 4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate (4-HFC-P) from glyceraldehyde-3-phosphate catalyzed by 4-HFC-P synthase.</title>
            <authorList>
                <person name="Wang Y."/>
                <person name="Jones M.K."/>
                <person name="Xu H."/>
                <person name="Ray W.K."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="25905665"/>
            <dbReference type="DOI" id="10.1021/acs.biochem.5b00176"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>PATHWAY</scope>
        <scope>ACTIVE SITE</scope>
        <scope>MUTAGENESIS OF ASP-25; LYS-27; LYS-85; ASP-151 AND LYS-155</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2014" name="Acta Crystallogr. F Struct. Biol. Commun." volume="70" first="1472" last="1479">
            <title>Structure of the methanofuran/methanopterin-biosynthetic enzyme MJ1099 from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Bobik T.A."/>
                <person name="Morales E.J."/>
                <person name="Shin A."/>
                <person name="Cascio D."/>
                <person name="Sawaya M.R."/>
                <person name="Arbing M."/>
                <person name="Yeates T.O."/>
                <person name="Rasche M.E."/>
            </authorList>
            <dbReference type="PubMed" id="25372812"/>
            <dbReference type="DOI" id="10.1107/S2053230X1402130X"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2 4">Catalyzes the formation of 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) from two molecules of glyceraldehyde-3-P (GA-3-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 4">
            <text>2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:83407"/>
            <dbReference type="EC" id="4.2.3.153"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <text evidence="4">kcat is 0.026 sec(-1).</text>
        </kinetics>
        <phDependence>
            <text evidence="4">Optimum pH is 7.0.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1 2 4">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer. Trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MfnB family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.153" evidence="1 4"/>
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        <property type="protein sequence ID" value="AAB99102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="PIR" id="B64437">
        <property type="entry name" value="B64437"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.40 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H/I=1-235"/>
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    <dbReference type="PDB" id="4U9P">
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        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B/C=1-235"/>
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    <dbReference type="PDBsum" id="4RC1"/>
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    <dbReference type="DNASU" id="1451996"/>
    <dbReference type="EnsemblBacteria" id="AAB99102">
        <property type="protein sequence ID" value="AAB99102"/>
        <property type="gene ID" value="MJ_1099"/>
    </dbReference>
    <dbReference type="GeneID" id="1451996"/>
    <dbReference type="KEGG" id="mja:MJ_1099"/>
    <dbReference type="eggNOG" id="arCOG04482">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068659_0_0_2"/>
    <dbReference type="InParanoid" id="Q58499"/>
    <dbReference type="OMA" id="NFPWVIR"/>
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    <dbReference type="BioCyc" id="MetaCyc:MON-18937"/>
    <dbReference type="BRENDA" id="4.2.3.153">
        <property type="organism ID" value="3260"/>
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    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00681">
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        <property type="entry name" value="4HFCP_synth"/>
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        <property type="entry name" value="4HFCP_synth_arc"/>
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    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UCP015957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000134865" description="(5-formylfuran-3-yl)methyl phosphate synthase">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="1 8">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1 8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Lack of activity." evidence="4">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Lack of activity." evidence="4">
        <original>K</original>
        <variation>R</variation>
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Lack of activity." evidence="4">
        <original>K</original>
        <variation>R</variation>
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Lack of activity." evidence="4">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="151"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Almost no change in activity." evidence="4">
        <original>K</original>
        <variation>R</variation>
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="2"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="22"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
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            <begin position="29"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="53"/>
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    <feature type="helix" evidence="10">
        <location>
            <begin position="66"/>
            <end position="78"/>
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    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="82"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="115"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="136"/>
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    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="146"/>
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    </feature>
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        <location>
            <begin position="160"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="166"/>
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    </feature>
    <feature type="strand" evidence="10">
        <location>
            <begin position="182"/>
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        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="191"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="194"/>
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    </feature>
    <feature type="helix" evidence="10">
        <location>
            <begin position="224"/>
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="24977328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25372812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="25905665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="5">
        <source>
            <dbReference type="PubMed" id="24977328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="6">
        <source>
            <dbReference type="PubMed" id="25905665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="7"/>
    <evidence type="ECO:0000305" key="8">
        <source>
            <dbReference type="PubMed" id="25905665"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="9">
        <source>
            <dbReference type="PDB" id="4RC1"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="10">
        <source>
            <dbReference type="PDB" id="4U9P"/>
        </source>
    </evidence>
    <sequence length="235" mass="25099" checksum="9F425AC7C6096E85" modified="1996-11-01" version="1">MILLVSPIDVEEAKEAIAGGADIIDVKNPKEGSLGANFPWMIKAIREVTPKDLLVSATVGDVPYKPGTISLAAVGAAISGADYIKVGLYGVKNYYQAVELMKNVVRAVKDIDENKIVVAAGYADAYRVGAVEPLIVPKIARDAGCDVAMLDTAIKDGKTLFDFQSKEILAEFVDEAHSYGLKCALAGSIKKEHIPILKEIGTDIVGVRGAACKGGDRNNGRIDRELVKELKELCK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-08-03" version="124" xmlns="http://uniprot.org/uniprot">
    <accession>Q58509</accession>
    <name>PYRE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="1">OPRT</shortName>
            <shortName evidence="1">OPRTase</shortName>
            <ecNumber evidence="1">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pyrE</name>
        <name type="ordered locus">MJ1109</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>diphosphate + orotidine 5'-phosphate = 5-phospho-alpha-D-ribose 1-diphosphate + orotate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="1"/>
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        <property type="gene ID" value="MJ_1109"/>
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    <dbReference type="GeneID" id="1452006"/>
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    <dbReference type="OrthoDB" id="117225at2157"/>
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        <property type="reaction ID" value="UER00119"/>
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        <property type="term" value="F:magnesium ion binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006222">
        <property type="term" value="P:UMP biosynthetic process"/>
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    </dbReference>
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        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PRibTrfase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="SSF53271"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000110779" description="Orotate phosphoribosyltransferase">
        <location>
            <begin position="1"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="116"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <sequence length="176" mass="19284" checksum="D208C1F49789E694" modified="1996-11-01" version="1">MDKKSKLINLLKEVGCIRFGEFILASGKKSNYYIDIKKATTNPEILKLVGEIIAEQIKDEDVKVAGVELGSVPIATAVSIIAQKPLLIVRKKPKDYGTKNKIEGELKEGDKVVIVEDVTTTGGSVLKAVKEIRENGGIVDKVFVVVDRLEGAKENLQKENVELIPLVTVKELQSTQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q58510</accession>
    <name>Y1110_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1110</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1110</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64438">
        <property type="entry name" value="E64438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870622.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58510"/>
    <dbReference type="SMR" id="Q58510"/>
    <dbReference type="STRING" id="243232.MJ_1110"/>
    <dbReference type="EnsemblBacteria" id="AAB99113">
        <property type="protein sequence ID" value="AAB99113"/>
        <property type="gene ID" value="MJ_1110"/>
    </dbReference>
    <dbReference type="GeneID" id="1452007"/>
    <dbReference type="KEGG" id="mja:MJ_1110"/>
    <dbReference type="eggNOG" id="arCOG03198">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_565769_0_0_2"/>
    <dbReference type="InParanoid" id="Q58510"/>
    <dbReference type="OMA" id="HKIAIGN"/>
    <dbReference type="OrthoDB" id="21208at2157"/>
    <dbReference type="PhylomeDB" id="Q58510"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007503">
        <property type="entry name" value="DUF530"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04409">
        <property type="entry name" value="DUF530"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107172" description="Uncharacterized protein MJ1110">
        <location>
            <begin position="1"/>
            <end position="486"/>
        </location>
    </feature>
    <sequence length="486" mass="56596" checksum="EC8007EAAEFFF977" modified="1996-11-01" version="1">MESTSNLIKNANEFLDSLNEINDKLKEVVGKIKNKTIDKTKLSDIILTLEKNLEILQDLKSKMEFLEFDSPYKNVGKLKGGYDSEGLQEIASYSTYLRRIASEKKGILERVRHALVAHKIALAHLTEDIGNINLPPNLPLDGSYKKIMFEFPPYLVTTYKEFLDILEPKGRGILTSYTISLIVIDKGKREFKRIKVEDKNYEKYIKEKFGNAIITSIKRNFSKNKIIDDQYVRRVLAIGYLNTYKDEIEKAINEKIDKLLNEEEKKYLNRYLELCLLFREEADISGGILDVRCMEERKLKELELKEILEKEGLYRDGEPIEPLKKAIKIKNELSKKISKDILIKRFSEDVFKFYLYKTPDERARSNLFPSIMITPQRGFLSWMSVDGINCVDVLDLKFKLEEELPKYQIPLKNIGGVALYLIHDWDAVERFNFKKKDIEDLLKKIALIEPIKEILKDKNVDVSKLEKFGKVKKEKTKKFLDLLSGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58604</accession>
    <name>Y1207_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized N-acetyltransferase MJ1207</fullName>
            <ecNumber>2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1207</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64450">
        <property type="entry name" value="F64450"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870719.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58604"/>
    <dbReference type="SMR" id="Q58604"/>
    <dbReference type="STRING" id="243232.MJ_1207"/>
    <dbReference type="PRIDE" id="Q58604"/>
    <dbReference type="DNASU" id="1452104"/>
    <dbReference type="EnsemblBacteria" id="AAB99211">
        <property type="protein sequence ID" value="AAB99211"/>
        <property type="gene ID" value="MJ_1207"/>
    </dbReference>
    <dbReference type="GeneID" id="1452104"/>
    <dbReference type="KEGG" id="mja:MJ_1207"/>
    <dbReference type="eggNOG" id="arCOG00826">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG01354">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_36_0_2"/>
    <dbReference type="InParanoid" id="Q58604"/>
    <dbReference type="OMA" id="VGFIVCD"/>
    <dbReference type="OrthoDB" id="59127at2157"/>
    <dbReference type="PhylomeDB" id="Q58604"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000074630" description="Uncharacterized N-acetyltransferase MJ1207">
        <location>
            <begin position="1"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="75"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="226" mass="26939" checksum="376E718D3509E2DA" modified="1996-11-01" version="1">MRVQLILHLEIPKEVCRSLEVDNYINNSIEINLKCEKKPTLYIKTHSIGSLKSILDDFFRCQNAAMEVYNLIKTYTIRNVTKDDLDDFLELYFKAYRGFDKYYYKKKKWARWYFKWLMKRDEDGFFVCEVNGKPVGFVACDCNWISNIEKREVAEIHEIFVDPDFRGRGIGTALINKAIEYAKKRGRRIVELWVGVENKGAIEFYKRLGFEEKEVVKGWLRMVKRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58606</accession>
    <name>MTM6_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type II methyltransferase M.MjaVI</fullName>
            <shortName evidence="1">M.MjaVI</shortName>
            <ecNumber>2.1.1.113</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>N-4 cytosine-specific methyltransferase MjaVI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mjaVIM</name>
        <name type="ordered locus">MJ1209</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">A beta subtype methylase that recognizes the double-stranded sequence 5'-CCGG-3', methylates C-1 on both strands, and protects the DNA from cleavage by the MjaVI endonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:16857"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:13674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:137933"/>
            <dbReference type="EC" id="2.1.1.113"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.113"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58606"/>
    <dbReference type="SMR" id="Q58606"/>
    <dbReference type="STRING" id="243232.MJ_1209"/>
    <dbReference type="REBASE" id="3893">
        <property type="entry name" value="M.MjaVI"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99220">
        <property type="protein sequence ID" value="AAB99220"/>
        <property type="gene ID" value="MJ_1209"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1209"/>
    <dbReference type="eggNOG" id="arCOG00115">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399756_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58606"/>
    <dbReference type="PRO" id="PR:Q58606"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015667">
        <property type="term" value="F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004809">
        <property type="term" value="F:tRNA (guanine-N2-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002941">
        <property type="entry name" value="DNA_methylase_N4/N6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017985">
        <property type="entry name" value="MeTrfase_CN4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001091">
        <property type="entry name" value="RM_Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01555">
        <property type="entry name" value="N6_N4_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00508">
        <property type="entry name" value="S21N4MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00093">
        <property type="entry name" value="N4_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000087939" description="Type II methyltransferase M.MjaVI">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000303" key="1">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="194" mass="22461" checksum="6DB2BAEEAEAEBC10" modified="1998-06-01" version="2">MKVIIVKIWNKINGITLINDDFLNVDLPNESIDLIVTSPPYNVGIDYNQHDDTIPYEEYLDWTKQWLKKALTLLKKDGRLCLNIPLDKNKGGIKPVYADIVKIALDVGFKYQTTIIWNEQNISRRTAWGSFMSASAPYVIAPVETIVVLYKESWKKLSKGESDITKEEFIEWTNGLWTFPGESKKELDIQHHFR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q58616</accession>
    <name>Y1219_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1219</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1219</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64452">
        <property type="entry name" value="B64452"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58616"/>
    <dbReference type="STRING" id="243232.MJ_1219"/>
    <dbReference type="EnsemblBacteria" id="AAB99224">
        <property type="protein sequence ID" value="AAB99224"/>
        <property type="gene ID" value="MJ_1219"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1219"/>
    <dbReference type="HOGENOM" id="CLU_2243864_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107221" description="Uncharacterized protein MJ1219">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="104" mass="11505" checksum="3306B9A4AC3C0E72" modified="1996-11-01" version="1">MVNMAPNTNFASLVAVAGCVLLGYNYYTGNIFCGVIGSLLLFGALWSLNGGKIWGIISFIISASIFCYINWDFILNLLFYSIIAFIVMSILILIFGNNRGGYYY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-08-03" version="132" xmlns="http://uniprot.org/uniprot">
    <accession>Q58663</accession>
    <name>LIVG_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable branched-chain amino acid transport ATP-binding protein LivG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">livG</name>
        <name type="ordered locus">MJ1267</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Structure" volume="9" first="571" last="586">
            <title>Crystal structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter.</title>
            <authorList>
                <person name="Karpowich N."/>
                <person name="Martsinkevich O."/>
                <person name="Millen L."/>
                <person name="Yuan Y.-R."/>
                <person name="Dai P.L."/>
                <person name="MacVey K."/>
                <person name="Thomas P.J."/>
                <person name="Hunt J.F."/>
            </authorList>
            <dbReference type="PubMed" id="11470432"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(01)00617-7"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text>Probable component of a branched-chain amino-acid transport system.</text>
    </comment>
    <comment type="subunit">
        <text>Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64458">
        <property type="entry name" value="B64458"/>
    </dbReference>
    <dbReference type="PDB" id="1G6H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=1-257"/>
    </dbReference>
    <dbReference type="PDB" id="1G9X">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B/C=1-257"/>
    </dbReference>
    <dbReference type="PDB" id="1GAJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A=1-257"/>
    </dbReference>
    <dbReference type="PDBsum" id="1G6H"/>
    <dbReference type="PDBsum" id="1G9X"/>
    <dbReference type="PDBsum" id="1GAJ"/>
    <dbReference type="AlphaFoldDB" id="Q58663"/>
    <dbReference type="BMRB" id="Q58663"/>
    <dbReference type="SMR" id="Q58663"/>
    <dbReference type="STRING" id="243232.MJ_1267"/>
    <dbReference type="EnsemblBacteria" id="AAB99273">
        <property type="protein sequence ID" value="AAB99273"/>
        <property type="gene ID" value="MJ_1267"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1267"/>
    <dbReference type="eggNOG" id="arCOG00925">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q58663"/>
    <dbReference type="OMA" id="EHDMRFI"/>
    <dbReference type="PhylomeDB" id="Q58663"/>
    <dbReference type="EvolutionaryTrace" id="Q58663"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0029">Amino-acid transport</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092412" description="Probable branched-chain amino acid transport ATP-binding protein LivG">
        <location>
            <begin position="1"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="8"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="6"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="20"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="29"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="35"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="60"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="76"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="84"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="92"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="99"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="105"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="114"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="144"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="151"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="173"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="180"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="186"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="205"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="213"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="217"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="221"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="230"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="238"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="248"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="253"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="1G6H"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1GAJ"/>
        </source>
    </evidence>
    <sequence length="257" mass="28995" checksum="B3347C3277D09C12" modified="1996-11-01" version="1">MRDTMEILRTENIVKYFGEFKALDGVSISVNKGDVTLIIGPNGSGKSTLINVITGFLKADEGRVYFENKDITNKEPAELYHYGIVRTFQTPQPLKEMTVLENLLIGEINPGESPLNSLFYKKWIPKEEEMVEKAFKILEFLKLSHLYDRKAGELSGGQMKLVEIGRALMTNPKMIVMDEPIAGVAPGLAHDIFNHVLELKAKGITFLIIEHRLDIVLNYIDHLYVMFNGQIIAEGRGEEEIKNVLSDPKVVEIYIGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58664</accession>
    <name>LIVF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable branched-chain amino acid transport ATP-binding protein LivF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">livF</name>
        <name type="ordered locus">MJ1268</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Probable component of a branched-chain amino-acid transport system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAB99274" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99274.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64458">
        <property type="entry name" value="C64458"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58664"/>
    <dbReference type="SMR" id="Q58664"/>
    <dbReference type="STRING" id="243232.MJ_1268"/>
    <dbReference type="EnsemblBacteria" id="AAB99274">
        <property type="protein sequence ID" value="AAB99274"/>
        <property type="gene ID" value="MJ_1268"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1268"/>
    <dbReference type="eggNOG" id="arCOG00924">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_2_2"/>
    <dbReference type="InParanoid" id="Q58664"/>
    <dbReference type="OMA" id="YITIVGP"/>
    <dbReference type="PhylomeDB" id="Q58664"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015803">
        <property type="term" value="P:branched-chain amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0029">Amino-acid transport</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000092405" description="Probable branched-chain amino acid transport ATP-binding protein LivF">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="1">
        <location>
            <begin position="2"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="34"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="237" mass="26353" checksum="5631954537C1E4F1" modified="2001-09-26" version="2">MIKVKNLNAGYGKLQILFDVNAKIEKGKITTVVGPNGSGKSTFLKTLFGLTKIYSGEIIFKDKDIAKVPPHQKARMKIAYLPQTNNVFANLTVEENLKIAGYVLDKDKVKERIEIALSVFPELKDILKRKAGTLSGGQRQFLAMGMALVRDAEVLMLDEPTAQLSPKLAEVIFEKIIEMRDNFGLTILLVEQNAKRALEISDNGYMFVSGRVAFEGTAEELLNHEKFKEYSLGITAV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58666</accession>
    <name>LIVM_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable branched-chain amino acid transport permease protein LivM</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">livM</name>
        <name type="ordered locus">MJ1270</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64458">
        <property type="entry name" value="E64458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870783.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58666"/>
    <dbReference type="STRING" id="243232.MJ_1270"/>
    <dbReference type="EnsemblBacteria" id="AAB99276">
        <property type="protein sequence ID" value="AAB99276"/>
        <property type="gene ID" value="MJ_1270"/>
    </dbReference>
    <dbReference type="GeneID" id="1452168"/>
    <dbReference type="KEGG" id="mja:MJ_1270"/>
    <dbReference type="eggNOG" id="arCOG01273">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031365_1_0_2"/>
    <dbReference type="InParanoid" id="Q58666"/>
    <dbReference type="OMA" id="PIFANKY"/>
    <dbReference type="OrthoDB" id="49280at2157"/>
    <dbReference type="PhylomeDB" id="Q58666"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="PTHR30482"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0029">Amino-acid transport</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060065" description="Probable branched-chain amino acid transport permease protein LivM">
        <location>
            <begin position="1"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="269"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="345" mass="37937" checksum="7771476C39F6FB51" modified="1996-11-01" version="1">MSIDLISMILLWFGLYYIVSLSLNMEFGYAGIPNFGKALSVLVGAIAVGGILDRLLMLYFGIGGDFITGTTYATSAINNLIASNPIVGIGILILAIILASILGFVVGAIFILPSAKLKEDYLGITLLAISEAVLLICTYNLNIIGGYYGISTPDILAFVSGEYRGWVFAWIVLFIAFLVYLFFERLLNTPFGRVLRAMRENENTVKAFGRDIMKLRIKTMAIGSAIGAIAGVLYSLYTVNIIANAFTRVDWTFFPFLMVLLGGKGNNKGVALGVLCYVIVKVLLDIYKYNIKYALGIPFEPVWLSYMLFGVLMLLILYYKPSGLIPEKPIITPPMKKKIMEISGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58673</accession>
    <name>LEUD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Isopropylmalate/citramalate isomerase small subunit</fullName>
            <ecNumber>4.2.1.33</ecNumber>
            <ecNumber>4.2.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>(R)-2-methylmalate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>(R)-citramalate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>3-isopropylmalate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Alpha-isopropylmalate dehydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Citraconate hydratase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Isopropylmalate isomerase</fullName>
            <shortName>IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Maleate hydratase</fullName>
            <shortName>Malease</shortName>
            <ecNumber>4.2.1.31</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">leuD</name>
        <name type="ordered locus">MJ1277</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="J. Bacteriol." volume="189" first="4391" last="4400">
            <title>Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Drevland R.M."/>
                <person name="Waheed A."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="17449626"/>
            <dbReference type="DOI" id="10.1128/JB.00166-07"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2012" name="Biochem. Biophys. Res. Commun." volume="419" first="160" last="164">
            <title>Crystal structure of LeuD from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Lee E.H."/>
                <person name="Cho Y.W."/>
                <person name="Hwang K.Y."/>
            </authorList>
            <dbReference type="PubMed" id="22326391"/>
            <dbReference type="DOI" id="10.1016/j.bbrc.2012.01.125"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Enzyme with broad specificity that catalyzes reversible hydroxyacid isomerizations via dehydration/hydration reactions. Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate, a step involved in leucine biosynthesis. Catalyzes the isomerization between 2-methylmalate and 3-methylmalate, via the formation of 2-methylmaleate (citraconate), a step involved in isoleucine biosynthesis. Also displays malease activity, i.e. catalyzes the hydration of maleate to form (R)-malate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(3R)-citramalate = 2-methylmaleate + H2O</text>
            <dbReference type="Rhea" id="RHEA:22332"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30719"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="EC" id="4.2.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-methylmaleate + H2O = (2R,3S)-3-methylmalate</text>
            <dbReference type="Rhea" id="RHEA:42424"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30719"/>
            <dbReference type="ChEBI" id="CHEBI:58511"/>
            <dbReference type="EC" id="4.2.1.35"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-malate = H2O + maleate</text>
            <dbReference type="Rhea" id="RHEA:23692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:30780"/>
            <dbReference type="EC" id="4.2.1.31"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1">80 uM for 2-methylmaleate</KM>
            <KM evidence="1">810 uM for (R)-2-methylmalate</KM>
            <KM evidence="1">1900 uM for racemic 2-isopropylmalate</KM>
            <KM evidence="1">39 uM for racemic 3-isopropylmalate</KM>
            <KM evidence="1">400 uM for maleate</KM>
            <Vmax evidence="1">15.0 umol/min/mg enzyme for 2-methylmaleate hydration reaction</Vmax>
            <Vmax evidence="1">1.5 umol/min/mg enzyme for (R)-2-methylmalate dehydration reaction</Vmax>
            <Vmax evidence="1">4.2 umol/min/mg enzyme for 2-isopropylmalate dehydration reaction</Vmax>
            <Vmax evidence="1">1.8 umol/min/mg enzyme for 3-isopropylmalate dehydration reaction</Vmax>
            <Vmax evidence="1">34.0 umol/min/mg enzyme for maleate hydration reaction</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="1">Optimum pH is 7.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="1">Optimum temperature is 70 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 LeuC and 2 LeuD. The heterotetramer that can be formed in vitro between HacA and LeuD is inactive.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LeuD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33"/>
    <dbReference type="EC" id="4.2.1.35"/>
    <dbReference type="EC" id="4.2.1.31"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64459">
        <property type="entry name" value="D64459"/>
    </dbReference>
    <dbReference type="PDB" id="3VBA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B/C/D/E/F=1-168"/>
    </dbReference>
    <dbReference type="PDBsum" id="3VBA"/>
    <dbReference type="AlphaFoldDB" id="Q58673"/>
    <dbReference type="SMR" id="Q58673"/>
    <dbReference type="STRING" id="243232.MJ_1277"/>
    <dbReference type="EnsemblBacteria" id="AAB99283">
        <property type="protein sequence ID" value="AAB99283"/>
        <property type="gene ID" value="MJ_1277"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1277"/>
    <dbReference type="eggNOG" id="arCOG02230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="InParanoid" id="Q58673"/>
    <dbReference type="OMA" id="GLPIIEC"/>
    <dbReference type="PhylomeDB" id="Q58673"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-13648"/>
    <dbReference type="BRENDA" id="4.2.1.33">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58673"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00067"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047508">
        <property type="term" value="F:(R)-2-methylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050075">
        <property type="term" value="F:maleate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword id="KW-0432">Leucine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141937" description="Isopropylmalate/citramalate isomerase small subunit">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="3"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="18"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="32"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="39"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="47"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="65"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="71"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="83"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="91"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="104"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="110"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="119"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="124"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="128"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="145"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="17449626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3VBA"/>
        </source>
    </evidence>
    <sequence length="168" mass="18377" checksum="A53C2CA883B6CCD7" modified="1996-11-01" version="1">MRSIIKGRVWKFGNNVDTDAILPARYLVYTKPEELAQFVMTGADPDFPKKVKPGDIIVGGKNFGCGSSREHAPLGLKGAGISCVIAESFARIFYRNAINVGLPLIECKGISEKVNEGDELEVNLETGEIKNLTTGEVLKGQKLPEFMMEILEAGGLMPYLKKKMAESQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q58684</accession>
    <name>Y1288_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1288</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1288</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64460">
        <property type="entry name" value="G64460"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58684"/>
    <dbReference type="SMR" id="Q58684"/>
    <dbReference type="STRING" id="243232.MJ_1288"/>
    <dbReference type="EnsemblBacteria" id="AAB99290">
        <property type="protein sequence ID" value="AAB99290"/>
        <property type="gene ID" value="MJ_1288"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1288"/>
    <dbReference type="eggNOG" id="arCOG01817">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1100963_0_0_2"/>
    <dbReference type="InParanoid" id="Q58684"/>
    <dbReference type="PhylomeDB" id="Q58684"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000207312" description="Uncharacterized protein MJ1288">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="252" mass="29481" checksum="F5418BCF81D510BC" modified="1998-12-15" version="2">MIFMVIKKIFGENFNFNKNIDIKKIFKLDKNVKKDREENESYLDALKEIYEEIKNLEIYEKMTIGMAEIIIGYDNVEKTKKYIVIEPILTKEEIKLFLKLRKVVQALLDVPVEEIDKEKLEDYLKEKIKEIFDDLKLTLDDVTRHKLIYFLIKYLIGYGKIDALMKDENLEDISCTGVGKPVYVFHRKYEHLKTNIKFETDEELDSFCISLAQRCGKSLTLANPIVDGSLPDGSRLNVTLGRDISDMVQHLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q58710</accession>
    <name>COBD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cobD</name>
        <name type="ordered locus">MJ1314</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CobD/CbiB family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64464">
        <property type="entry name" value="A64464"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870831.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58710"/>
    <dbReference type="STRING" id="243232.MJ_1314"/>
    <dbReference type="EnsemblBacteria" id="AAB99321">
        <property type="protein sequence ID" value="AAB99321"/>
        <property type="gene ID" value="MJ_1314"/>
    </dbReference>
    <dbReference type="GeneID" id="1452216"/>
    <dbReference type="KEGG" id="mja:MJ_1314"/>
    <dbReference type="eggNOG" id="arCOG04274">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054212_0_2_2"/>
    <dbReference type="InParanoid" id="Q58710"/>
    <dbReference type="OMA" id="NSGYTMA"/>
    <dbReference type="OrthoDB" id="101304at2157"/>
    <dbReference type="PhylomeDB" id="Q58710"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="PTHR34308"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00380">
        <property type="entry name" value="cobD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000150941" description="Probable cobalamin biosynthesis protein CobD">
        <location>
            <begin position="1"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="307" mass="34502" checksum="D24DDA2B50ECC1B1" modified="1996-11-01" version="1">MLNPIILFLAIIFDRIIGELPESIHPTVWIGKLIAFLENIFKSTNCKNKYRDFLFGSLTTFITLLVVGVIAFFVDKCIMLLPFPLNYIIYGFLLSTTIGYKSLFEFCKKPIEYIKNGDLEGARKAVQHIVSRDASKLDKEHVLSAAVESLSENITDSIIGALFYAIFFGLPGAFVYRAINTLDAMIGYKNEKYLWYGKLAARLDDIANFIPSRIAGILLIITAPFYKGDVKKAIYGFLKEANKVPSPNSGYTMATLANALNITLEKIGYYKLGSGKIDVEKSLNAFKAVDYTVVVFLIIYTLIWWIT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58711</accession>
    <name>PRNK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Polyribonucleotide 5'-hydroxyl-kinase MJ1315</fullName>
            <ecNumber>2.7.1.78</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Polynucleotide kinase MJ1315</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1315</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-monophospho-2'-deoxyribonucleoside-DNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15669"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13184"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-monophospho-ribonucleoside-RNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54580"/>
            <dbReference type="Rhea" id="RHEA-COMP:13935"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="2.7.1.78"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64464">
        <property type="entry name" value="B64464"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58711"/>
    <dbReference type="SMR" id="Q58711"/>
    <dbReference type="STRING" id="243232.MJ_1315"/>
    <dbReference type="DNASU" id="1452217"/>
    <dbReference type="EnsemblBacteria" id="AAB99322">
        <property type="protein sequence ID" value="AAB99322"/>
        <property type="gene ID" value="MJ_1315"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1315"/>
    <dbReference type="eggNOG" id="arCOG04127">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051301_0_1_2"/>
    <dbReference type="InParanoid" id="Q58711"/>
    <dbReference type="OMA" id="RKIHREM"/>
    <dbReference type="PhylomeDB" id="Q58711"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046404">
        <property type="term" value="F:polydeoxyribonucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051731">
        <property type="term" value="F:polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="PTHR12755"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107272" description="Polyribonucleotide 5'-hydroxyl-kinase MJ1315">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="39"/>
            <end position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <sequence length="361" mass="41352" checksum="E2397969BE7E8870" modified="1996-11-01" version="1">MVDNMISKAYYTTEIPEDRFEALSCIKDSQKPLKIILLGGVDSGKTTLATFLANELLNLGFKVAIVDSDVGQKSILPPATISLAFPETNFNNLYEIKPYKSYFVGSTAPIQFFGEMITGTKLLCDYAEDKADIIIVDTTGLISGSGADLKRMKIEMIKPDIIIALEKRNELKSILKPFENKIRVFYLKVYENAKSFSREERKEIRAEKWKEYFKNSKIYNIGFNDVVIGGTKVFQGEKILEDEKYLLESLFKWKILYGSKCDGRYTIVKRDLVNMPRQIDKNILYYIEPERFNNLIVGLIDEDSFCIGLGILKTIDFENETLEILTPISEEDIKNIREIRFGRIRVDENGEELGLLDRDSI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58726</accession>
    <name>Y1330_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1330</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1330</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64466">
        <property type="entry name" value="A64466"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58726"/>
    <dbReference type="SMR" id="Q58726"/>
    <dbReference type="STRING" id="243232.MJ_1330"/>
    <dbReference type="EnsemblBacteria" id="AAB99339">
        <property type="protein sequence ID" value="AAB99339"/>
        <property type="gene ID" value="MJ_1330"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1330"/>
    <dbReference type="eggNOG" id="arCOG01869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_120791_0_0_2"/>
    <dbReference type="InParanoid" id="Q58726"/>
    <dbReference type="OMA" id="YDAKKPG"/>
    <dbReference type="PhylomeDB" id="Q58726"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="SSF101307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107277" description="Uncharacterized protein MJ1330">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <sequence length="127" mass="14151" checksum="F33BC568A9CDF378" modified="1998-06-01" version="2">MDAGMDLYIGSNEERDRVKEKLKEILLKQLSNPNVSTLLIAAILLDNEGRANNLPFNYNEDPNYVYVDEVIGLAIANEIAGTKAIFNFRFYDAKKPGIIGELDRKGFMFLDDAIAGLLAGCMSKVFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-08-03" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>Q58735</accession>
    <name>Y1339_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1339</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1339</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To M.thermoautotrophicum MTH765.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64467">
        <property type="entry name" value="B64467"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870857.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58735"/>
    <dbReference type="SMR" id="Q58735"/>
    <dbReference type="STRING" id="243232.MJ_1339"/>
    <dbReference type="EnsemblBacteria" id="AAB99349">
        <property type="protein sequence ID" value="AAB99349"/>
        <property type="gene ID" value="MJ_1339"/>
    </dbReference>
    <dbReference type="GeneID" id="1452242"/>
    <dbReference type="KEGG" id="mja:MJ_1339"/>
    <dbReference type="eggNOG" id="arCOG00362">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077970_2_1_2"/>
    <dbReference type="InParanoid" id="Q58735"/>
    <dbReference type="OMA" id="RFEFMRE"/>
    <dbReference type="OrthoDB" id="80071at2157"/>
    <dbReference type="PhylomeDB" id="Q58735"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016433">
        <property type="entry name" value="Small_GTPase_MJ1339"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004882">
        <property type="entry name" value="GTP_bind_MJ1339_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107282" description="Uncharacterized protein MJ1339">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="G">
        <location>
            <begin position="17"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="12"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="154" mass="17196" checksum="94626C30C181107A" modified="1996-11-01" version="1">MKKDEVKVVVIGSSDVGKTTLMENLIDKIGKVEYKGITTAIDYGSLTIKDKKIHFFGTPGQKRFEFMRELALKGTNFALVVLDASKGITKEDEEIIKLLESKKIPYGIFINKTDVGDIDTSEVYNFCNPKFIVKGCAVKKDGLDELINKIMSHT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-08-03" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58740</accession>
    <name>GLNK2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nitrogen regulatory protein GlnK2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">glnK2</name>
        <name type="ordered locus">MJ1344</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the regulation of nitrogen metabolism (By similarity). Regulates the activity of its targets by protein-protein interaction in response to the nitrogen status of the cell (By similarity). Regulates the activity of the ammonia channel Amt2 via direct interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer (By similarity). Interacts and forms a complex with Amt2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the P(II) protein family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64467">
        <property type="entry name" value="G64467"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870862.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58740"/>
    <dbReference type="SMR" id="Q58740"/>
    <dbReference type="STRING" id="243232.MJ_1344"/>
    <dbReference type="EnsemblBacteria" id="AAB99353">
        <property type="protein sequence ID" value="AAB99353"/>
        <property type="gene ID" value="MJ_1344"/>
    </dbReference>
    <dbReference type="GeneID" id="1452247"/>
    <dbReference type="KEGG" id="mja:MJ_1344"/>
    <dbReference type="eggNOG" id="arCOG02305">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082268_0_0_2"/>
    <dbReference type="InParanoid" id="Q58740"/>
    <dbReference type="OMA" id="YRGTEHV"/>
    <dbReference type="OrthoDB" id="113533at2157"/>
    <dbReference type="PhylomeDB" id="Q58740"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030234">
        <property type="term" value="F:enzyme regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002187">
        <property type="entry name" value="N-reg_PII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017918">
        <property type="entry name" value="N-reg_PII_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30115">
        <property type="entry name" value="PTHR30115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00543">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00340">
        <property type="entry name" value="PIIGLNB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00938">
        <property type="entry name" value="P-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="SSF54913"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00638">
        <property type="entry name" value="PII_GLNB_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51343">
        <property type="entry name" value="PII_GLNB_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139816" description="Nitrogen regulatory protein GlnK2">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="37"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ADP</name>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="87"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="B8ZYW0"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O28524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q60381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="112" mass="12532" checksum="3DDCE2A0F32FE9F3" modified="1996-11-01" version="1">MKKVEAIIRPEKLEIVKKALSDAGYVGMTVSEVKGRGVQGGIVERYRGREYIVDLIPKVKIELVVKEEDVDNVIDIICENARTGNPGDGKIFVIPVERVVRVRTKEEGRDVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-10-18" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58753</accession>
    <name>Y1358_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1358</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1358</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64469">
        <property type="entry name" value="E64469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870875.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58753"/>
    <dbReference type="SMR" id="Q58753"/>
    <dbReference type="STRING" id="243232.MJ_1358"/>
    <dbReference type="DNASU" id="1452260"/>
    <dbReference type="EnsemblBacteria" id="AAB99377">
        <property type="protein sequence ID" value="AAB99377"/>
        <property type="gene ID" value="MJ_1358"/>
    </dbReference>
    <dbReference type="GeneID" id="1452260"/>
    <dbReference type="KEGG" id="mja:MJ_1358"/>
    <dbReference type="eggNOG" id="arCOG12720">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1718223_0_0_2"/>
    <dbReference type="OMA" id="VDEHNIA"/>
    <dbReference type="OrthoDB" id="137199at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107296" description="Uncharacterized protein MJ1358">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="152" mass="18007" checksum="0FB45F8E0C4378FC" modified="1998-06-01" version="2">MGVVFAFGFYLIFIKLTGLKLMDYFPRFKENRLKMIFSILSVILAFLINWLIMKNFSFLIEIIHPIASVWIFIILIYLLLRFLFLKRVPLSNYEKKFMGNMSAIAIFLELLKIIEYVDEHNIASPITVALVFFIPVVVFFNCKYFYEMELSS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-15" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58754</accession>
    <name>Y1359_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0273 protein MJ1359</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1359</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0273 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64469">
        <property type="entry name" value="F64469"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870876.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58754"/>
    <dbReference type="SMR" id="Q58754"/>
    <dbReference type="STRING" id="243232.MJ_1359"/>
    <dbReference type="EnsemblBacteria" id="AAB99368">
        <property type="protein sequence ID" value="AAB99368"/>
        <property type="gene ID" value="MJ_1359"/>
    </dbReference>
    <dbReference type="GeneID" id="1452261"/>
    <dbReference type="KEGG" id="mja:MJ_1359"/>
    <dbReference type="eggNOG" id="arCOG01171">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023669_2_0_2"/>
    <dbReference type="InParanoid" id="Q58754"/>
    <dbReference type="OMA" id="FSQQFLW"/>
    <dbReference type="OrthoDB" id="76011at2157"/>
    <dbReference type="PhylomeDB" id="Q58754"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01076">
        <property type="entry name" value="UPF0273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022475">
        <property type="entry name" value="UPF0273_KaiC-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03877">
        <property type="entry name" value="thermo_KaiC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184585" description="UPF0273 protein MJ1359">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="KaiC" evidence="1">
        <location>
            <begin position="2"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01076"/>
        </source>
    </evidence>
    <sequence length="242" mass="26988" checksum="28F86D37CB25811B" modified="1996-11-01" version="1">MKRVKTGIPGMDEILHGGIPERNVVLLSGGPGTGKSIFCQQFLYKGVVDYNEPSILVALEEHPVQIRENMRQFGWDIRKLEEEGKFAIIDAFTYGIGSAAKREKYVVNDPNDERELIDVLKTAINDIGAKRIGIDSVTTLYINKPMLARRTVFLLKRVISGLGCTAIFTSQISVGERGFGGPGVEHAVDGIIRLDLDEIDGELKRSLIVWKMRGTSHSLKRHPFDITNEGIIVYPDKVLKLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58774</accession>
    <name>Y1379_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative protein adenylyltransferase MJ1379</fullName>
            <ecNumber evidence="1">2.7.7.n1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative antitoxin MJ1379</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1379</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable antitoxin component of a putative type VII toxin-antitoxin (TA) system. Neutralizes cognate toxic MJ1380 by di-AMPylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + L-tyrosyl-[protein] = diphosphate + O-(5'-adenylyl)-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.n1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + O-(5'-adenylyl)-L-tyrosyl-[protein] = diphosphate + O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">Binds 2 Mg(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Probably forms a complex with cognate toxin MJ1380.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MntA antitoxin family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.n1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64472">
        <property type="entry name" value="B64472"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870896.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58774"/>
    <dbReference type="SMR" id="Q58774"/>
    <dbReference type="STRING" id="243232.MJ_1379"/>
    <dbReference type="EnsemblBacteria" id="AAB99389">
        <property type="protein sequence ID" value="AAB99389"/>
        <property type="gene ID" value="MJ_1379"/>
    </dbReference>
    <dbReference type="GeneID" id="1452282"/>
    <dbReference type="KEGG" id="mja:MJ_1379"/>
    <dbReference type="eggNOG" id="arCOG01206">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130257_10_3_2"/>
    <dbReference type="InParanoid" id="Q58774"/>
    <dbReference type="OMA" id="TMNPFLK"/>
    <dbReference type="OrthoDB" id="108083at2157"/>
    <dbReference type="PhylomeDB" id="Q58774"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000107304" description="Putative protein adenylyltransferase MJ1379">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GSX(10)DXD motif" evidence="2">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJN8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH7"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="100" mass="11819" checksum="E20512C0CFE1019F" modified="1997-11-01" version="1">MKTLSEIKEILRKHKKILKDKYKVKSIALFGSYARGEQTEESDIDIMVEFDENNYPSFSEYLELIEYLEKILGLKVDLITKKSIHNPYVKKSIEEDLIYV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58795</accession>
    <name>Y1400_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0333 protein MJ1400</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1400</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0333 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64474">
        <property type="entry name" value="G64474"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870917.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58795"/>
    <dbReference type="SMR" id="Q58795"/>
    <dbReference type="STRING" id="243232.MJ_1400"/>
    <dbReference type="EnsemblBacteria" id="AAB99411">
        <property type="protein sequence ID" value="AAB99411"/>
        <property type="gene ID" value="MJ_1400"/>
    </dbReference>
    <dbReference type="GeneID" id="1452303"/>
    <dbReference type="KEGG" id="mja:MJ_1400"/>
    <dbReference type="eggNOG" id="arCOG05082">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_197251_2_0_2"/>
    <dbReference type="OMA" id="NTHNTAM"/>
    <dbReference type="OrthoDB" id="129266at2157"/>
    <dbReference type="PhylomeDB" id="Q58795"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000218268" description="UPF0333 protein MJ1400">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="67" mass="7485" checksum="30B893EF421AAC34" modified="1996-11-01" version="1">MKFIMKFIKSNKGQISLEFSLLVMVVVLSAIIVSYYLIKTAIETRNANMDVINQSSNVAEKSLSNVT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58827</accession>
    <name>Y1432_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1432</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1432</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64478">
        <property type="entry name" value="G64478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870950.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58827"/>
    <dbReference type="SMR" id="Q58827"/>
    <dbReference type="STRING" id="243232.MJ_1432"/>
    <dbReference type="EnsemblBacteria" id="AAB99442">
        <property type="protein sequence ID" value="AAB99442"/>
        <property type="gene ID" value="MJ_1432"/>
    </dbReference>
    <dbReference type="GeneID" id="1452336"/>
    <dbReference type="KEGG" id="mja:MJ_1432"/>
    <dbReference type="eggNOG" id="arCOG00985">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090468_1_1_2"/>
    <dbReference type="InParanoid" id="Q58827"/>
    <dbReference type="OMA" id="HDHIEIL"/>
    <dbReference type="OrthoDB" id="85279at2157"/>
    <dbReference type="PhylomeDB" id="Q58827"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022430">
        <property type="entry name" value="CHP03684"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015266">
        <property type="entry name" value="DUF1947"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="PTHR22798"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09183">
        <property type="entry name" value="DUF1947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005067">
        <property type="entry name" value="Tma_RNA-bind_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="SSF88697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03684">
        <property type="entry name" value="arCOG00985"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107324" description="Uncharacterized protein MJ1432">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="78"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00161"/>
        </source>
    </evidence>
    <sequence length="162" mass="18365" checksum="C67CF3638B2024BD" modified="1996-11-01" version="1">MEIRKRYFLSKKDVKKIKKELEVFFENVDEIIPKKGNVEIAITDDFEIILVDKEPIAFKKDDKVIPTLKLLLKSLPDKNLVVVDIGAIKFLINGADVMAPGIVDADENIKEEDVVFVVDENHKKPICVGIALMNGKEMKEADKGKAIKNLHYVGDKIWNFKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q58853</accession>
    <name>Y1458_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1458</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1458</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64482">
        <property type="entry name" value="A64482"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870978.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58853"/>
    <dbReference type="SMR" id="Q58853"/>
    <dbReference type="STRING" id="243232.MJ_1458"/>
    <dbReference type="EnsemblBacteria" id="AAB99466">
        <property type="protein sequence ID" value="AAB99466"/>
        <property type="gene ID" value="MJ_1458"/>
    </dbReference>
    <dbReference type="GeneID" id="1452362"/>
    <dbReference type="KEGG" id="mja:MJ_1458"/>
    <dbReference type="eggNOG" id="arCOG00813">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1264587_0_0_2"/>
    <dbReference type="OMA" id="YTQQFIK"/>
    <dbReference type="OrthoDB" id="89825at2157"/>
    <dbReference type="PhylomeDB" id="Q58853"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040537">
        <property type="entry name" value="DUF5612"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015832">
        <property type="entry name" value="UCP006363_ACT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18462">
        <property type="entry name" value="DUF5612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006363">
        <property type="entry name" value="UCP006363_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="SSF52172"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107346" description="Uncharacterized protein MJ1458">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="4"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01007"/>
        </source>
    </evidence>
    <sequence length="218" mass="23472" checksum="DD96DA57FFCB8A78" modified="1996-11-01" version="1">MEIGISIEAENKVGVLHKLTGILSELGGNITYTQQFIKDDGKIGFIYMEVEGIKDIEELKRRMESCECVKSFEIHSSLKKIYGKRVIIIGGGAQVAEVARGAISEADRHNIRGERISVDTLPIVGEENLYEAVKAVATLPRVGILVLAGSLMGGKITEAVKELKEKTGIPVISLKMFGSVPKVADLVVGDPLQAGVLAVMAIAETAKFDINKVKGRVL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q58904</accession>
    <name>Y1509_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1509</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1509</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64488">
        <property type="entry name" value="D64488"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871032.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58904"/>
    <dbReference type="SMR" id="Q58904"/>
    <dbReference type="STRING" id="243232.MJ_1509"/>
    <dbReference type="EnsemblBacteria" id="AAB99534">
        <property type="protein sequence ID" value="AAB99534"/>
        <property type="gene ID" value="MJ_1509"/>
    </dbReference>
    <dbReference type="GeneID" id="1452416"/>
    <dbReference type="KEGG" id="mja:MJ_1509"/>
    <dbReference type="eggNOG" id="arCOG05087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117658_0_0_2"/>
    <dbReference type="InParanoid" id="Q58904"/>
    <dbReference type="OMA" id="IKMRHIP"/>
    <dbReference type="OrthoDB" id="84048at2157"/>
    <dbReference type="PhylomeDB" id="Q58904"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015207">
        <property type="entry name" value="DUF1931"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09123">
        <property type="entry name" value="DUF1931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="SSF47113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107383" description="Uncharacterized protein MJ1509">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <sequence length="153" mass="17718" checksum="0B1AABEDC831685B" modified="1996-11-01" version="1">MAEMIIPYPQLKKIMKTTCEIDLYKTEAEDIMDVVEKKLADLFEVAHRNAKEENAKIIKMRHIPLTKGFLNSMELFRSVIEEENIVAETIKKYVMKKIPGDLPLDDIVVDNLPLITGTIFIVVGRVIKALHEDIERIRKEHIEEAKKVLDYTL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="116" xmlns="http://uniprot.org/uniprot">
    <accession>Q58916</accession>
    <name>NAH2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable Na(+)/H(+) antiporter 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>MjNhaP2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1521</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="FEBS Lett." volume="527" first="245" last="249">
            <title>Identification of a pH regulated Na(+)/H(+) antiporter of Methanococcus jannaschii.</title>
            <authorList>
                <person name="Hellmer J."/>
                <person name="Paetzold R."/>
                <person name="Zeilinger C."/>
            </authorList>
            <dbReference type="PubMed" id="12220668"/>
            <dbReference type="DOI" id="10.1016/S0014-5793(02)03244-1"/>
        </citation>
        <scope>SIMILARITY TO NA(+)/H(+) ANTIPORTERS</scope>
    </reference>
    <comment type="function">
        <text>This is probably a Na(+)/H(+) antiporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64489">
        <property type="entry name" value="H64489"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871045.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58916"/>
    <dbReference type="SMR" id="Q58916"/>
    <dbReference type="STRING" id="243232.MJ_1521"/>
    <dbReference type="EnsemblBacteria" id="AAB99540">
        <property type="protein sequence ID" value="AAB99540"/>
        <property type="gene ID" value="MJ_1521"/>
    </dbReference>
    <dbReference type="GeneID" id="1452429"/>
    <dbReference type="KEGG" id="mja:MJ_1521"/>
    <dbReference type="eggNOG" id="arCOG01961">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005912_9_3_2"/>
    <dbReference type="InParanoid" id="Q58916"/>
    <dbReference type="OMA" id="WFDEYGE"/>
    <dbReference type="OrthoDB" id="47913at2157"/>
    <dbReference type="PhylomeDB" id="Q58916"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015299">
        <property type="term" value="F:solute:proton antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0050">Antiport</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0915">Sodium</keyword>
    <keyword id="KW-0739">Sodium transport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000052403" description="Probable Na(+)/H(+) antiporter 2">
        <location>
            <begin position="1"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="93"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="242"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="307"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="384"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="422" mass="45810" checksum="AF56570C0626D339" modified="1998-12-15" version="2">MNIVLFLGYLSILFAGGAIIAKIAKKIGIPDIPLLLIFGLILSILNVIPKNIVESSFDFIGNFGLIILLFIGSFEMEWNIMKRVLDVIIKLDILALLIVWIISGIVFNFVFHLPILSLIGLLFGAIVSATDPATLIPIFSSMDIDPEVAITLEAESVFNDPLGIVVTLICLSALGLAKAENPILEFFSLAVGGIILGVIAGKFYEIIISKIKFEDYIAPFTLGLAIAFWYFAEGIFPSITGYEISGFMAVAIMGLYIGNVIVHKKEHKKDMEKVAVFMDELSIFIRILIFVLLGASISIPLLEKYALPAFLCALGSILLARPVGVLIATAIPPIRPLTERIYLALEGPRGVVPATLAAMVYTEIMKHPNIVPKNIASLMPPTELAGTILVATFMTIIVSVVLEASWAKPLANILLKRKTSTS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q58924</accession>
    <name>OGT_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methylated-DNA--protein-cysteine methyltransferase</fullName>
            <ecNumber evidence="1 2">2.1.1.63</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">6-O-methylguanine-DNA methyltransferase</fullName>
            <shortName evidence="1">MGMT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">O-6-methylguanine-DNA-alkyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ogt</name>
        <name type="ordered locus">MJ1529</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Magn. Reson. Chem." volume="44" first="S71" last="S82">
            <title>Structural studies of MJ1529, an O6-methylguanine-DNA methyltransferase.</title>
            <authorList>
                <person name="Roberts A."/>
                <person name="Pelton J.G."/>
                <person name="Wemmer D.E."/>
            </authorList>
            <dbReference type="PubMed" id="16826543"/>
            <dbReference type="DOI" id="10.1002/mrc.1823"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:24000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:11367"/>
            <dbReference type="Rhea" id="RHEA-COMP:11368"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:85445"/>
            <dbReference type="ChEBI" id="CHEBI:85448"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]</text>
            <dbReference type="Rhea" id="RHEA:53428"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:10132"/>
            <dbReference type="Rhea" id="RHEA-COMP:13555"/>
            <dbReference type="Rhea" id="RHEA-COMP:13556"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:82612"/>
            <dbReference type="ChEBI" id="CHEBI:137386"/>
            <dbReference type="ChEBI" id="CHEBI:137387"/>
            <dbReference type="EC" id="2.1.1.63"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text>This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MGMT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.63" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64490">
        <property type="entry name" value="H64490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871053.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2G7H">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-167"/>
    </dbReference>
    <dbReference type="PDBsum" id="2G7H"/>
    <dbReference type="AlphaFoldDB" id="Q58924"/>
    <dbReference type="BMRB" id="Q58924"/>
    <dbReference type="SMR" id="Q58924"/>
    <dbReference type="STRING" id="243232.MJ_1529"/>
    <dbReference type="EnsemblBacteria" id="AAB99547">
        <property type="protein sequence ID" value="AAB99547"/>
        <property type="gene ID" value="MJ_1529"/>
    </dbReference>
    <dbReference type="GeneID" id="1452437"/>
    <dbReference type="KEGG" id="mja:MJ_1529"/>
    <dbReference type="eggNOG" id="arCOG02724">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000445_52_2_2"/>
    <dbReference type="InParanoid" id="Q58924"/>
    <dbReference type="OMA" id="DYFIGMI"/>
    <dbReference type="OrthoDB" id="122341at2157"/>
    <dbReference type="PhylomeDB" id="Q58924"/>
    <dbReference type="EvolutionaryTrace" id="Q58924"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003908">
        <property type="term" value="F:methylated-DNA-[protein]-cysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006307">
        <property type="term" value="P:DNA dealkylation involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00772">
        <property type="entry name" value="OGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001497">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023546">
        <property type="entry name" value="MGMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="SSF46767"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00374">
        <property type="entry name" value="MGMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000139377" description="Methylated-DNA--protein-cysteine methyltransferase">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile; methyl group acceptor" evidence="1">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="2"/>
            <end position="5"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="8"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="31"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="44"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="67"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="82"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="99"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="110"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="123"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="127"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16826543"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2G7H"/>
        </source>
    </evidence>
    <sequence length="167" mass="19431" checksum="7FC44483D31F6EF2" modified="1996-11-01" version="1">MIIQIEEYFIGMIFKGNQLVRNTIPLRREEIFNFMDGEVVSNPEDEHLKVAEIILKLYFAEIDDKKVRELISYKLEVPEFTKKVLDIVKDIEFGKTLTYGDIAKKLNTSPRAVGMALKRNPLPLIIPCHRVVAKNSLGGYSYGLDKKKFILERERLNMVSFKFNKVY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-11" modified="2022-05-25" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q58925</accession>
    <name>Y1530_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized N-acetyltransferase MJ1530</fullName>
            <ecNumber>2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1530</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64491">
        <property type="entry name" value="A64491"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871054.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58925"/>
    <dbReference type="SMR" id="Q58925"/>
    <dbReference type="STRING" id="243232.MJ_1530"/>
    <dbReference type="EnsemblBacteria" id="AAB99551">
        <property type="protein sequence ID" value="AAB99551"/>
        <property type="gene ID" value="MJ_1530"/>
    </dbReference>
    <dbReference type="GeneID" id="1452438"/>
    <dbReference type="KEGG" id="mja:MJ_1530"/>
    <dbReference type="eggNOG" id="arCOG00833">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013985_23_0_2"/>
    <dbReference type="InParanoid" id="Q58925"/>
    <dbReference type="OMA" id="SGEIMGY"/>
    <dbReference type="OrthoDB" id="95090at2157"/>
    <dbReference type="PhylomeDB" id="Q58925"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:peptide alpha-N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006474">
        <property type="term" value="P:N-terminal protein amino acid acetylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="PTHR23091"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="SSF55729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0012">Acyltransferase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000074632" description="Uncharacterized N-acetyltransferase MJ1530">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="156" mass="18052" checksum="298FE51AC69E88CD" modified="1996-11-01" version="1">MIIRKFSSKDLDAVEEIEREAFKTPYPTSLILGFWSMYPNCFYVAEIDGRVVGYILGSMDWGNGHIISLAVKKECRGLGIGTALLKTLENYYFNIANCNYIVLEVRVSNVLARRFYYRMGYRDRKLLPKYYEDGEDAILMIKKKPNAKGPLIITLW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q58926</accession>
    <name>T1SX_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Putative type I restriction enzyme MjaX specificity subunit</fullName>
            <shortName>S protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Putative type I specificity subunit S.MjaX</fullName>
            <shortName evidence="2">S.MjaX</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1531</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">A putative specificity (S) subunit of a type I restriction enzyme thought to recognize 5'-TAGN(6)TGC-3'; the other subunits are unknown.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Contains two DNA recognition domains, each specifying recognition of one of the two defined components of the target sequence.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64491">
        <property type="entry name" value="B64491"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58926"/>
    <dbReference type="SMR" id="Q58926"/>
    <dbReference type="STRING" id="243232.MJ_1531"/>
    <dbReference type="REBASE" id="3899">
        <property type="entry name" value="S.MjaX"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99552">
        <property type="protein sequence ID" value="AAB99552"/>
        <property type="gene ID" value="MJ_1531"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1531"/>
    <dbReference type="eggNOG" id="arCOG02626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021095_2_1_2"/>
    <dbReference type="InParanoid" id="Q58926"/>
    <dbReference type="OMA" id="GARRYVY"/>
    <dbReference type="PhylomeDB" id="Q58926"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <feature type="chain" id="PRO_0000107393" description="Putative type I restriction enzyme MjaX specificity subunit">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P05719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="425" mass="48889" checksum="DB879AC2D8911AE9" modified="1997-11-01" version="1">MVILLQFYKEENFKKTEIGEIPEDWEVRELKDILEVIRNGLTAKQNKDKIGYPITRIETISDSKIDITKLGYVEDIKQEDIAKYRLIIGDILFSHINSEEHIGKVAIYEGKPEFLLHGMNLLLLRPNKNKIEPYYLLYLLRHFKQKNIFKYIAKRAVNQSSINQTQLKHLKIPLPPLEEQKQIAKILSDFDNLIGTINKQIEVLNKAKKGMMKKLFTKGVFEHKSFKKSEIGEIPEDWEVVELGNEKYFKIIMGQSPPSSSYNKEGEGVPFLQGKAEFGNIYPNPVLYTNKPLKVVDDEDILISVRAPVGDVNIAPFKLCIGRGLAGIKSNKEKVDNFFVFYYLSYIKPKIEYLGGGAVFKAITKKDLESIKIPLPPLEEQKAIAKRLKAIDDLIEIKRKEKEQIEKAKKKIMNLLLTGKIRVKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q59006</accession>
    <name>AMYA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative alpha-amylase</fullName>
            <ecNumber>3.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1611</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64501">
        <property type="entry name" value="B64501"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871136.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59006"/>
    <dbReference type="SMR" id="Q59006"/>
    <dbReference type="STRING" id="243232.MJ_1611"/>
    <dbReference type="CAZy" id="GH57">
        <property type="family name" value="Glycoside Hydrolase Family 57"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99631">
        <property type="protein sequence ID" value="AAB99631"/>
        <property type="gene ID" value="MJ_1611"/>
    </dbReference>
    <dbReference type="GeneID" id="1452520"/>
    <dbReference type="KEGG" id="mja:MJ_1611"/>
    <dbReference type="eggNOG" id="arCOG03278">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033691_0_0_2"/>
    <dbReference type="InParanoid" id="Q59006"/>
    <dbReference type="OMA" id="GWRSPNY"/>
    <dbReference type="OrthoDB" id="45403at2157"/>
    <dbReference type="PhylomeDB" id="Q59006"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="SSF88713"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0326">Glycosidase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184575" description="Putative alpha-amylase">
        <location>
            <begin position="1"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="467" mass="55558" checksum="40A6B1CDDD4D967E" modified="1996-11-01" version="1">MLITFNFEVHQPHRLNKEINQNGNTLWEKYVDTKLNKEVFNKVANKCYIPTNELILELIDEYDFKVNYSITGVFVEQALEFNDYVLDLFKDLVKTGNVELIAETYHHSLTSLFETEDEFIEDIEMHRKMYKEIFGFKAKVFRNTELIYNNRIAKIAKDLGFKAIFTEGIEKILGWRSPNYLYQSPDGMKILLRNYRLSDDIGFRFSARDWDQYPLTADKYAIWLASTPGEVINIYMDYETFGEHHWKETGIFEFLRYLPIEIAKHEHLEVVNVSEVVDRLEPRGEIYVHEFATISWADTERDVSAWLGNKMQRISFEKLKDIGKFIKENSNKLKKLNKFDEIYKMYKVLQTSDNLYYQSIKGLSDMSVHNYFSHFDTPFDAYASYLNILYDFEYYIKELLAKSEFDKNNRRKDGQKQYEKDDEVKKESLINTNIIVAKDDKTESIYIEDEEGKKNKRYERDEGFIIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q59027</accession>
    <name>Y1633_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1633</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1633</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64503">
        <property type="entry name" value="G64503"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59027"/>
    <dbReference type="SMR" id="Q59027"/>
    <dbReference type="STRING" id="243232.MJ_1633"/>
    <dbReference type="PRIDE" id="Q59027"/>
    <dbReference type="EnsemblBacteria" id="AAB99654">
        <property type="protein sequence ID" value="AAB99654"/>
        <property type="gene ID" value="MJ_1633"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1633"/>
    <dbReference type="eggNOG" id="arCOG01566">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046377_1_1_2"/>
    <dbReference type="InParanoid" id="Q59027"/>
    <dbReference type="OMA" id="KCATALM"/>
    <dbReference type="PhylomeDB" id="Q59027"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="SSF64182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107448" description="Uncharacterized protein MJ1633">
        <location>
            <begin position="1"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="4"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00543"/>
        </source>
    </evidence>
    <sequence length="478" mass="52989" checksum="555CDADF98BD109D" modified="1996-11-01" version="1">MMNMITVIGFGSFGRKVVNFIKNKEPITIIDKNIDDADDLVKEGVTVIVGDATQDEVLKKAKIENADIVLILTNEPEVNRRIAERVCELSPNSYKIARAIPRYPELYMGLNIDKIINILESGAKDIAKEVEDAKLKRKLMQLKSVLIEGKKRCMKLEKTEEEKKAPLLILTHINPDPDAIASAMALKTLAERWGVDSDIAYGGNIGYDENKAMINLLGIKLLNVEDIDLDNYCVIAVIDTSTSKQLPIELPNIDIIIDHHNNTDLTAKYMDVRPEVGATASILTQYLMELDIEPSRNLATALFYGIQSDTDYFKRETSKLDFEAAAYLQSYIDASILNMIENPEISTEVMEVLAKAVMNRRVVKGNIALAYVGEISNRDALPKAADFLLKMEGISTTFVFGIVGDEIHISARTKDLRLNLGEILNKAFGGGGHQTAAAAKIPLGIFKAVSDKEALRKLVEEAIRAKILEVIGIKEEEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q59034</accession>
    <name>TRMY_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (pseudouridine(54)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmY</name>
        <name type="ordered locus">MJ1640</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2012" name="RNA" volume="18" first="412" last="420">
            <title>Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs.</title>
            <authorList>
                <person name="Wurm J.P."/>
                <person name="Griese M."/>
                <person name="Bahr U."/>
                <person name="Held M."/>
                <person name="Heckel A."/>
                <person name="Karas M."/>
                <person name="Soppa J."/>
                <person name="Woehnert J."/>
            </authorList>
            <dbReference type="PubMed" id="22274954"/>
            <dbReference type="DOI" id="10.1261/rna.028498.111"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2012" name="RNA" volume="18" first="421" last="433">
            <title>The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA.</title>
            <authorList>
                <person name="Chatterjee K."/>
                <person name="Blaby I.K."/>
                <person name="Thiaville P.C."/>
                <person name="Majumder M."/>
                <person name="Grosjean H."/>
                <person name="Yuan Y.A."/>
                <person name="Gupta R."/>
                <person name="de Crecy-Lagard V."/>
            </authorList>
            <dbReference type="PubMed" id="22274953"/>
            <dbReference type="DOI" id="10.1261/rna.030841.111"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>GENE NAME</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="J. Mol. Cell Biol." volume="2" first="366" last="374">
            <title>Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase.</title>
            <authorList>
                <person name="Chen H.Y."/>
                <person name="Yuan Y.A."/>
            </authorList>
            <dbReference type="PubMed" id="21098051"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-METHIONINE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3 4">Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3 4">
            <text>pseudouridine(54) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:55292"/>
            <dbReference type="Rhea" id="RHEA-COMP:14140"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="EC" id="2.1.1.257"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily. TrmY family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.257" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64504">
        <property type="entry name" value="F64504"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871164.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3AI9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.55 A"/>
        <property type="chains" value="X=1-205"/>
    </dbReference>
    <dbReference type="PDB" id="3AIA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="A/B=1-205"/>
    </dbReference>
    <dbReference type="PDBsum" id="3AI9"/>
    <dbReference type="PDBsum" id="3AIA"/>
    <dbReference type="AlphaFoldDB" id="Q59034"/>
    <dbReference type="SMR" id="Q59034"/>
    <dbReference type="STRING" id="243232.MJ_1640"/>
    <dbReference type="EnsemblBacteria" id="AAB99660">
        <property type="protein sequence ID" value="AAB99660"/>
        <property type="gene ID" value="MJ_1640"/>
    </dbReference>
    <dbReference type="GeneID" id="1452549"/>
    <dbReference type="KEGG" id="mja:MJ_1640"/>
    <dbReference type="eggNOG" id="arCOG01239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107018_0_0_2"/>
    <dbReference type="InParanoid" id="Q59034"/>
    <dbReference type="OMA" id="HADHCII"/>
    <dbReference type="OrthoDB" id="126445at2157"/>
    <dbReference type="PhylomeDB" id="Q59034"/>
    <dbReference type="BRENDA" id="2.1.1.257">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q59034"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd18087">
        <property type="entry name" value="TrmY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00587">
        <property type="entry name" value="tRNA_methyltr_TrmY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007158">
        <property type="entry name" value="TrmY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703">
        <property type="entry name" value="PTHR40703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04013">
        <property type="entry name" value="Methyltrn_RNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000157948" description="tRNA (pseudouridine(54)-N(1))-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <begin position="179"/>
            <end position="184"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 2">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="2"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="22"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="43"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="50"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="64"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="101"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="106"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="112"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="132"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="150"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="163"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="175"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="186"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="21098051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="22274953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="22274954"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3AIA"/>
        </source>
    </evidence>
    <sequence length="205" mass="23704" checksum="EBED16985B07E132" modified="1996-11-01" version="1">MREFIFKANKTITSSDINLKDLPGSCGRLDLLCRCVSDAFFLSHDIRRDVVFYAVLYGQPNPPVCIKFVGSELKKVSPDERNIAIFIKKALKKFEELDEEQRKDWNQSTPGIYVRRLGFRNLVLEKLEEGKNIYYLHMNGEDVENVDIENPVFIIGDHIGIGEEDERFLDEIKAKRISLSPLELHANHCITIIHNVLDKKRICEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q59061</accession>
    <name>CSM5_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system Cms protein Csm5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR type III A-associated protein Csm5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">csm5</name>
        <name type="ordered locus">MJ1667</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities.</text>
    </comment>
    <comment type="function">
        <text evidence="1">This subunit might be involved in maturation of a crRNA intermediate to its mature form.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the Csm effector complex that includes Cas10, Csm2, Csm3, Csm4 and Csm5.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="2">Encoded in a type III-A CRISPR locus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm5 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64508">
        <property type="entry name" value="A64508"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59061"/>
    <dbReference type="STRING" id="243232.MJ_1667"/>
    <dbReference type="PRIDE" id="Q59061"/>
    <dbReference type="EnsemblBacteria" id="AAB99692">
        <property type="protein sequence ID" value="AAB99692"/>
        <property type="gene ID" value="MJ_1667"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1667"/>
    <dbReference type="eggNOG" id="arCOG03718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036878_3_0_2"/>
    <dbReference type="InParanoid" id="Q59061"/>
    <dbReference type="OMA" id="PIFIGCG"/>
    <dbReference type="PhylomeDB" id="Q59061"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010173">
        <property type="entry name" value="CRISPR-assoc_Csm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38007">
        <property type="entry name" value="PTHR38007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01899">
        <property type="entry name" value="cas_TM1807_csm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000107464" description="CRISPR system Cms protein Csm5">
        <location>
            <begin position="1"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0A7HF79"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="418" mass="48804" checksum="14C31107463948D2" modified="1996-11-01" version="1">MLKTYPLKMMMTSRIRIMKTTINLIIRSIPMENQYYSHLIQRGITMEVKCELITPIFIGCGEEYSQLDYFIEDGLAHIIDLEKAVSDLDDLEKVDYISGLIVSNIDNNRLNLTAKDILESVGLNPYDYVIRKIESEIFSNKKTRVKKFINQNNTYYIPGSSIKGAIRTAYIFNYYDKNLPELLKILDDRNIKLHDKGKELEKNAISKDIPKDFFKYLKISDSLNLEGEFKFIHTKRWNYRKKKFDVPINMEGMTKGTFSINIKIEDEFFKNINKRLKTNYNPKDDEKKFDILKNLCNNFSKTVVEFELKKNNPVYVEKSYEKLLADINKDDAIYLNLGFGGGFLNKTVYPLLWKNDENHLYFRKIKSLFIALSGGNKNLKNAWLKANSYLDFPTTKTVYVKNNSAIAPLGWIKMTLVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q59062</accession>
    <name>CSM4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR system Cms protein Csm4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR type III-A associated RAMP protein Csm4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">csm4</name>
        <name type="ordered locus">MJ1668</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2015" name="J. Mol. Biol." volume="427" first="259" last="273">
            <title>Crystal structure of the Csm3-Csm4 subcomplex in the type III-A CRISPR-Cas interference complex.</title>
            <authorList>
                <person name="Numata T."/>
                <person name="Inanaga H."/>
                <person name="Sato C."/>
                <person name="Osawa T."/>
            </authorList>
            <dbReference type="PubMed" id="25451598"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2014.09.029"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF 229-GLU-ASP-230</scope>
        <scope>RNA-BINDING</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities.</text>
    </comment>
    <comment type="function">
        <text evidence="3 5">The subunit probably binds to the 5' handle of the crRNA, helping in discrimination between self- and non-self (Probable). The Csm3-Csm4 complex binds both crRNA and a non-specific RNA; Csm4 alone also binds RNA (PubMed:25451598).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Part of the Csm effector complex that includes Cas10, Csm2, Csm3, Csm4 and Csm5 (By similarity). Stable Cas10/Csm1-Csm4, Csm3-Csm4 (which crystallizes as 2 heterodimers) and Cas10-Csm1-Csm3-Csm4 subcomplexes can be isolated (PubMed:25451598).</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">Encoded in a type III-A CRISPR locus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CRISPR-associated Csm4 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64508">
        <property type="entry name" value="B64508"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871192.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4QTS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.10 A"/>
        <property type="chains" value="A/B=1-376"/>
    </dbReference>
    <dbReference type="PDBsum" id="4QTS"/>
    <dbReference type="AlphaFoldDB" id="Q59062"/>
    <dbReference type="SMR" id="Q59062"/>
    <dbReference type="STRING" id="243232.MJ_1668"/>
    <dbReference type="PRIDE" id="Q59062"/>
    <dbReference type="EnsemblBacteria" id="AAB99688">
        <property type="protein sequence ID" value="AAB99688"/>
        <property type="gene ID" value="MJ_1668"/>
    </dbReference>
    <dbReference type="GeneID" id="1452577"/>
    <dbReference type="KEGG" id="mja:MJ_1668"/>
    <dbReference type="eggNOG" id="arCOG03222">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062371_0_0_2"/>
    <dbReference type="InParanoid" id="Q59062"/>
    <dbReference type="OMA" id="NSKYHFG"/>
    <dbReference type="OrthoDB" id="68179at2157"/>
    <dbReference type="PhylomeDB" id="Q59062"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005510">
        <property type="entry name" value="Csm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040932">
        <property type="entry name" value="Csm4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17953">
        <property type="entry name" value="Csm4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01903">
        <property type="entry name" value="cas5_csm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000107465" description="CRISPR system Cms protein Csm4">
        <location>
            <begin position="1"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="332"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No longer interacts with Csm3." evidence="3">
        <original>ED</original>
        <variation>AA</variation>
        <location>
            <begin position="229"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="2"/>
            <end position="8"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="72"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="81"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="95"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="102"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="105"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="111"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="118"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="122"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="turn" evidence="7">
        <location>
            <begin position="132"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="139"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="154"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="202"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="228"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="244"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="254"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="265"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="270"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="284"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="288"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="293"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="304"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="310"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="321"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="364"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0A7HGA1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25451598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="25451598"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="4QTS"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="4QTS"/>
        </source>
    </evidence>
    <sequence length="376" mass="43851" checksum="F5DC850CA5FF7DF2" modified="1996-11-01" version="1">MKMVVLKPKINSKFHFGEGSLERNSKIFHSNSLFSAIVNNYIKLYGREDLEKNIEKIKNIRLSSLLYKIKNIYLIPKPEHPEFYKLKGNPGIKPKDIKKIQFFSIKAYKELLDNELDWKNKIKHIVDYQTINKSIVISEKEIEEIKRIFGIKAEKLKHAKISLISKHLEQKVAIDRLKDITLEKDDKGQLYNIEFIKLNENVEFYFLIDYNNEDKEFIKKLEASIKLIEDEGLGGKRSIGAGFFEKVEIVDLPEDFNEILDENSKYNNLEYKMLLGVGIPNKDDIKNIEYYKLIEIGGYIYSLECLTKPKRNILALTEGSIVKNDFIGDVKDISPQNDDDEQNKNNENNNKLNHKVYTHGKPILLPFNPKRDNYGS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q60180</accession>
    <name>TRPA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tryptophan synthase alpha chain</fullName>
            <ecNumber evidence="1">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trpA</name>
        <name type="ordered locus">MJ1038</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + H2O + L-tryptophan</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TrpA family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99041" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99041.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64429">
        <property type="entry name" value="E64429"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60180"/>
    <dbReference type="SMR" id="Q60180"/>
    <dbReference type="STRING" id="243232.MJ_1038"/>
    <dbReference type="EnsemblBacteria" id="AAB99041">
        <property type="protein sequence ID" value="AAB99041"/>
        <property type="gene ID" value="MJ_1038"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1038"/>
    <dbReference type="eggNOG" id="arCOG01086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016734_0_2_2"/>
    <dbReference type="InParanoid" id="Q60180"/>
    <dbReference type="OMA" id="LVMTYWN"/>
    <dbReference type="PhylomeDB" id="Q60180"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04724">
        <property type="entry name" value="Tryptophan_synthase_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00131">
        <property type="entry name" value="Trp_synth_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018204">
        <property type="entry name" value="Trp_synthase_alpha_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002028">
        <property type="entry name" value="Trp_synthase_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43406">
        <property type="entry name" value="PTHR43406"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00290">
        <property type="entry name" value="Trp_syntA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00262">
        <property type="entry name" value="trpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00167">
        <property type="entry name" value="TRP_SYNTHASE_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="chain" id="PRO_0000098889" description="Tryptophan synthase alpha chain">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="281" mass="31271" checksum="D68E352004FB1E9F" modified="2000-05-30" version="2">MIMKLAEKFEELKNKGEKAFVAFYVGGDPNLEISEKALEVICKHADIVEIGIPFSDPVADGITIQKADVRALNSGMNPLKAFELAKKLNEKAPNVPKVFLTYYNIIFKMGEEEFVKKCKEAGVSGIIVPDLPIEEADSLYNYCKKYGVDLIFLVAPTTPDERLKKILEKCSGFVYVVSVTGITGAREKVAEETKELIKRVKKFSKIPACVGFGISKREHVEEITEIADGAIVGSAIVKIVEKHLDENGQIKDEEKFLKELEEFVKNLKEGTKKKAKVAIKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="56" xmlns="http://uniprot.org/uniprot">
    <accession>Q60268</accession>
    <name>Y3503_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL03</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL03</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64510">
        <property type="entry name" value="C64510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890050.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60268"/>
    <dbReference type="SMR" id="Q60268"/>
    <dbReference type="STRING" id="243232.MJ_ECL03"/>
    <dbReference type="EnsemblBacteria" id="AAC37077">
        <property type="protein sequence ID" value="AAC37077"/>
        <property type="gene ID" value="MJ_ECL03"/>
    </dbReference>
    <dbReference type="GeneID" id="1450790"/>
    <dbReference type="KEGG" id="mja:MJ_ECL03"/>
    <dbReference type="HOGENOM" id="CLU_2191037_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107495" description="Uncharacterized protein MJECL03">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <sequence length="108" mass="12603" checksum="36E6408CDEFA49EB" modified="1997-11-01" version="1">MRDCKVQVGGFGEWIIKCDLELTFDEVMRGEIEELVEFIKSLESLLKSSSEDSFRKNVLNALISTLKTLPWTSRLEYADDEHNIIDVYPDDKNKKWIIKCNLCLFPDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q60270</accession>
    <name>Y3508_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable helicase MJECL08</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ordered locus">MJECL08</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HerA family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64510">
        <property type="entry name" value="H64510"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890055.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_ECL08"/>
    <dbReference type="EnsemblBacteria" id="AAC37081">
        <property type="protein sequence ID" value="AAC37081"/>
        <property type="gene ID" value="MJ_ECL08"/>
    </dbReference>
    <dbReference type="GeneID" id="1450794"/>
    <dbReference type="KEGG" id="mja:MJ_ECL08"/>
    <dbReference type="eggNOG" id="arCOG00280">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023842_2_0_2"/>
    <dbReference type="InParanoid" id="Q60270"/>
    <dbReference type="OMA" id="WINRIAK"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="PhylomeDB" id="Q60270"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018538">
        <property type="entry name" value="HerA_barrel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033186">
        <property type="entry name" value="HerA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="PTHR42957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05872">
        <property type="entry name" value="DUF853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09378">
        <property type="entry name" value="HAS-barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107500" description="Probable helicase MJECL08">
        <location>
            <begin position="1"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="171"/>
            <end position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="501"/>
            <end position="502"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="F2Z5Z6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q97WG8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000312" key="4">
        <source>
            <dbReference type="EMBL" id="AAC37081.1"/>
        </source>
    </evidence>
    <sequence length="520" mass="58182" checksum="D1888AFDFE1D9079" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
    <accession>Q60273</accession>
    <name>Y3511_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL11</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64511">
        <property type="entry name" value="C64511"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890058.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60273"/>
    <dbReference type="STRING" id="243232.MJ_ECL11"/>
    <dbReference type="EnsemblBacteria" id="AAC37084">
        <property type="protein sequence ID" value="AAC37084"/>
        <property type="gene ID" value="MJ_ECL11"/>
    </dbReference>
    <dbReference type="GeneID" id="1450797"/>
    <dbReference type="KEGG" id="mja:MJ_ECL11"/>
    <dbReference type="eggNOG" id="arCOG03239">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115497_0_0_2"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107503" description="Uncharacterized protein MJECL11">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <sequence length="114" mass="12826" checksum="99A168C6C180676B" modified="1997-11-01" version="1">MGEGFKSALIIALLTSILKNGYLLIDSAEAFHHPSSLEITSQMLTKSVKNNNVQVFLTTHSLELIDFLLEHASKEGIEGRLIYMRRDGENLISSMESFENVREMRETLGIDLRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="110" xmlns="http://uniprot.org/uniprot">
    <accession>Q60297</accession>
    <name>T1MH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Type I restriction enzyme MjaIX methylase subunit</fullName>
            <shortName>M protein</shortName>
            <ecNumber evidence="1">2.1.1.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Type I methyltransferase M.MjaIX</fullName>
            <shortName evidence="4">M.MjaIX</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL42</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
        <scope>SUBTYPE</scope>
    </reference>
    <comment type="function">
        <text evidence="1 4">The subtype gamma methyltransferase (M) subunit of a type I restriction enzyme. The M and S subunits together form a methyltransferase (MTase) that methylates A-3 on the top and A-2 on the bottom strand of the sequence 5'-CCAN(5)GTR-3'. In the presence of the R subunit the complex can also act as an endonuclease, binding to the same target sequence but cutting the DNA some distance from this site. Whether the DNA is cut or modified depends on the methylation state of the target sequence. When the target site is unmodified, the DNA is cut. When the target site is hemimethylated, the complex acts as a maintenance MTase modifying the DNA so that both strands become methylated. After locating a non-methylated recognition site, the enzyme complex serves as a molecular motor that translocates DNA in an ATP-dependent manner until a collision occurs that triggers cleavage.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The type I restriction/modification system is composed of three polypeptides R, M and S.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Type I restriction and modification enzymes are complex, multifunctional systems which require ATP, S-adenosyl methionine and Mg(2+) as cofactors and, in addition to their endonucleolytic and methylase activities, are potent DNA-dependent ATPases.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the N(4)/N(6)-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="1"/>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64515">
        <property type="entry name" value="A64515"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60297"/>
    <dbReference type="SMR" id="Q60297"/>
    <dbReference type="STRING" id="243232.MJ_ECL42"/>
    <dbReference type="REBASE" id="155513">
        <property type="entry name" value="M.VscVS05ORF3158P"/>
    </dbReference>
    <dbReference type="REBASE" id="188606">
        <property type="entry name" value="M.AsoACEORF2384P"/>
    </dbReference>
    <dbReference type="REBASE" id="191852">
        <property type="entry name" value="M.Apa1447ORF2799P"/>
    </dbReference>
    <dbReference type="REBASE" id="191856">
        <property type="entry name" value="M.Apa1447ORF3031P"/>
    </dbReference>
    <dbReference type="REBASE" id="191865">
        <property type="entry name" value="M.Apa1342ORF2767P"/>
    </dbReference>
    <dbReference type="REBASE" id="191868">
        <property type="entry name" value="M.Apa1342ORF2943P"/>
    </dbReference>
    <dbReference type="REBASE" id="191872">
        <property type="entry name" value="M.Apa1342ORF3157P"/>
    </dbReference>
    <dbReference type="REBASE" id="191885">
        <property type="entry name" value="M.Apa1468ORF2991P"/>
    </dbReference>
    <dbReference type="REBASE" id="191888">
        <property type="entry name" value="M.Apa1468ORF3072P"/>
    </dbReference>
    <dbReference type="REBASE" id="204130">
        <property type="entry name" value="M.Keu1446ORF147P"/>
    </dbReference>
    <dbReference type="REBASE" id="211764">
        <property type="entry name" value="M.RspL182ORF2217P"/>
    </dbReference>
    <dbReference type="REBASE" id="3910">
        <property type="entry name" value="M.MjaIX"/>
    </dbReference>
    <dbReference type="DNASU" id="1450824"/>
    <dbReference type="EnsemblBacteria" id="AAC37111">
        <property type="protein sequence ID" value="AAC37111"/>
        <property type="gene ID" value="MJ_ECL42"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_ECL42"/>
    <dbReference type="eggNOG" id="arCOG02632">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013049_4_1_2"/>
    <dbReference type="InParanoid" id="Q60297"/>
    <dbReference type="OMA" id="YEYLMGM"/>
    <dbReference type="PhylomeDB" id="Q60297"/>
    <dbReference type="PRO" id="PR:Q60297"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022749">
        <property type="entry name" value="D12N6_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12161">
        <property type="entry name" value="HsdM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000088029" description="Type I restriction enzyme MjaIX methylase subunit">
        <location>
            <begin position="1"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="227"/>
            <end position="232"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="256"/>
            <end position="258"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P08957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q89Z59"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5"/>
    <sequence length="558" mass="64552" checksum="0DD8568C71C3AF37" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="73" xmlns="http://uniprot.org/uniprot">
    <accession>Q60306</accession>
    <name>Y3407_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS07</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS07</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64516">
        <property type="entry name" value="G64516"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890100.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60306"/>
    <dbReference type="STRING" id="243232.MJ_ECS07"/>
    <dbReference type="PRIDE" id="Q60306"/>
    <dbReference type="EnsemblBacteria" id="AAC37065">
        <property type="protein sequence ID" value="AAC37065"/>
        <property type="gene ID" value="MJ_ECS07"/>
    </dbReference>
    <dbReference type="GeneID" id="1450834"/>
    <dbReference type="KEGG" id="mja:MJ_ECS07"/>
    <dbReference type="eggNOG" id="arCOG04110">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_620571_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107488" description="Uncharacterized protein MJECS07">
        <location>
            <begin position="1"/>
            <end position="441"/>
        </location>
    </feature>
    <sequence length="441" mass="53092" checksum="5A494246188B0104" modified="1997-11-01" version="1">METTIMVVAMDRLTYYKLTSRYLYYFCLDRLLSCHRLDLDRDRKINWRHPKYWNYNNIPKGEKPYLRHIDLWDYRGNFKDWIIENLRSIYCSPYYFESPRYELEYPAIDYNYWDWVIDIDFKGNFKKSKEIAKEVREIFKDYGVKDIHIKFSGSKGFHIWINARYLPNEILNKGYVEGSTILTNFLNFKLRKLLDFVGKEGDTGIDLSIYKENMTVTAPFSLYYADIEGKTPISIPFPINKVEKFKPIYLQNVKPYFIRKLTKYYENFGEIRGDLTDFVDDALRYYQATKKVNNDIKIDLEINTNKKAKPKVEHSKREYIKTTIGDKEVVLNKVKLESYLNELINSNWEDGKMRGCYYLTSLCKLLGYGRDKTIKLLNRWASKYGNKYIKHIDYEVRYLYDRNGKVCSIKWLKENIPEAKTQIEIYKKYYELDKNNYLVKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q60310</accession>
    <name>Y3411_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0304 protein MJECS11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS11</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0304 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAC37069" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37069.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64517">
        <property type="entry name" value="C64517"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890104.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60310"/>
    <dbReference type="SMR" id="Q60310"/>
    <dbReference type="PRIDE" id="Q60310"/>
    <dbReference type="EnsemblBacteria" id="AAC37069">
        <property type="protein sequence ID" value="AAC37069"/>
        <property type="gene ID" value="MJ_ECS11"/>
    </dbReference>
    <dbReference type="GeneID" id="1450838"/>
    <dbReference type="KEGG" id="mja:MJ_ECS11"/>
    <dbReference type="HOGENOM" id="CLU_101021_1_0_2"/>
    <dbReference type="InParanoid" id="Q60310"/>
    <dbReference type="PhylomeDB" id="Q60310"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3190.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00762">
        <property type="entry name" value="UPF0304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005587">
        <property type="entry name" value="UPF0304_YfbU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023146">
        <property type="entry name" value="YfbU_alpha-helical_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023145">
        <property type="entry name" value="YfbU_helix-hairpin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03887">
        <property type="entry name" value="YfbU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006272">
        <property type="entry name" value="UCP006272"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000218176" description="UPF0304 protein MJECS11">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="168" mass="20255" checksum="71AE55175D60F70F" modified="2004-04-13" version="2">MLSKIERLILANQYKILKILENTSEYDEIIKILEEGYEIFYDEILGHIFDELPESEGQFVLDILSFYDIVVEPYKQKNPNDHEIINHPYSYFKGFDGNSETKYMAFVRFLIEDQKKFSFVAKYAKKTDNFNSHFPMLDKYRKMVELWESKYNKKYDLKREEILDILNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q60372</accession>
    <name>Y068_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0212 protein MJ0068</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0068</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0212 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64308">
        <property type="entry name" value="D64308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869560.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60372"/>
    <dbReference type="STRING" id="243232.MJ_0068"/>
    <dbReference type="EnsemblBacteria" id="AAB98047">
        <property type="protein sequence ID" value="AAB98047"/>
        <property type="gene ID" value="MJ_0068"/>
    </dbReference>
    <dbReference type="GeneID" id="8804275"/>
    <dbReference type="KEGG" id="mja:MJ_0068"/>
    <dbReference type="eggNOG" id="arCOG02119">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138334_0_0_2"/>
    <dbReference type="InParanoid" id="Q60372"/>
    <dbReference type="OMA" id="KVFNAQS"/>
    <dbReference type="OrthoDB" id="101789at2157"/>
    <dbReference type="PhylomeDB" id="Q60372"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01223">
        <property type="entry name" value="UPF0212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007564">
        <property type="entry name" value="UPF0212"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42199">
        <property type="entry name" value="PTHR42199"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04475">
        <property type="entry name" value="DUF555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016934">
        <property type="entry name" value="UCP016934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000068276" description="UPF0212 protein MJ0068">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01223"/>
        </source>
    </evidence>
    <sequence length="115" mass="12515" checksum="2FE056FFC3A9A076" modified="1997-11-01" version="1">MPNYHVTLQAAYIVRNVDDVEDAISVTISQIGKMLNKEGLNYVDIDIGLTICPKCGELVDCVLVVARTALVGVLLSMKVFNAESPEHAIRIAKATIGKVLKNIPLEPVDVVELEK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>P54026</accession>
    <name>RS17E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps17e</name>
        <name type="ordered locus">MJ0245</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98233" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98233.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64330">
        <property type="entry name" value="F64330"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496434.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54026"/>
    <dbReference type="SMR" id="P54026"/>
    <dbReference type="STRING" id="243232.MJ_0245"/>
    <dbReference type="EnsemblBacteria" id="AAB98233">
        <property type="protein sequence ID" value="AAB98233"/>
        <property type="gene ID" value="MJ_0245"/>
    </dbReference>
    <dbReference type="GeneID" id="1451099"/>
    <dbReference type="KEGG" id="mja:MJ_0245"/>
    <dbReference type="eggNOG" id="arCOG01885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_176720_0_1_2"/>
    <dbReference type="InParanoid" id="P54026"/>
    <dbReference type="OMA" id="TRDFEHN"/>
    <dbReference type="OrthoDB" id="128227at2157"/>
    <dbReference type="PhylomeDB" id="P54026"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_S17e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018273">
        <property type="entry name" value="Ribosomal_S17e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="RPS17e-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="PTHR10732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="SSF116820"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00712">
        <property type="entry name" value="RIBOSOMAL_S17E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000141553" description="30S ribosomal protein S17e">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="62" mass="7510" checksum="7D0DA547702EF62E" modified="2001-12-19" version="2">MGRIRQTLIKRTAMELIKKYRDLFTTDFETNKRVLEEVAQISTKRLRNRIAGYITHKMRQLQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>P54036</accession>
    <name>RS17_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps17</name>
        <name type="ordered locus">MJ0465</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64358">
        <property type="entry name" value="A64358"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869965.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54036"/>
    <dbReference type="SMR" id="P54036"/>
    <dbReference type="STRING" id="243232.MJ_0465"/>
    <dbReference type="EnsemblBacteria" id="AAB98454">
        <property type="protein sequence ID" value="AAB98454"/>
        <property type="gene ID" value="MJ_0465"/>
    </dbReference>
    <dbReference type="GeneID" id="1451327"/>
    <dbReference type="KEGG" id="mja:MJ_0465"/>
    <dbReference type="eggNOG" id="arCOG04096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073626_0_3_2"/>
    <dbReference type="InParanoid" id="P54036"/>
    <dbReference type="OMA" id="KKYERYE"/>
    <dbReference type="OrthoDB" id="108145at2157"/>
    <dbReference type="PhylomeDB" id="P54036"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_S17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_S17/S11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_S17_arc-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_S17_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_S17_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="PTHR10744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000128499" description="30S ribosomal protein S17">
        <location>
            <begin position="1"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <sequence length="117" mass="13266" checksum="476CFF0D79EF5D8C" modified="1996-10-01" version="1">MAARNIGIQVKAPEVECDDKNCPFHGNLPVRGQSFVGVVVSDKPHKTVIIKREVVKYIKKYERYERRTTKLAAHNPPCIHARVGDIVRVMECRPISKTKAFVVVEKLGRIDEVKGEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P54047</accession>
    <name>RL15_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl15</name>
        <name type="ordered locus">MJ0477</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64359">
        <property type="entry name" value="E64359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869978.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54047"/>
    <dbReference type="SMR" id="P54047"/>
    <dbReference type="STRING" id="243232.MJ_0477"/>
    <dbReference type="EnsemblBacteria" id="AAB98466">
        <property type="protein sequence ID" value="AAB98466"/>
        <property type="gene ID" value="MJ_0477"/>
    </dbReference>
    <dbReference type="GeneID" id="1451339"/>
    <dbReference type="KEGG" id="mja:MJ_0477"/>
    <dbReference type="eggNOG" id="arCOG00779">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109163_0_0_2"/>
    <dbReference type="InParanoid" id="P54047"/>
    <dbReference type="OMA" id="WGRVGQH"/>
    <dbReference type="OrthoDB" id="121171at2157"/>
    <dbReference type="PhylomeDB" id="P54047"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_prot_L15/27a_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_L15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000104863" description="50S ribosomal protein L15">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="143" mass="16107" checksum="2A78A8ABA578FF87" modified="1996-10-01" version="1">MIRKKKKVKKIRGSRTCGGGSHKKRRGAGNKGGRGMAGGHKHKWTWIIKYCPDYFGKYGFKRHPSLVKRLETINVGELEEIVLKNPDKFEKEDDKFVVDVIELGYEKVLGKGKVTIPMIVKAVEVSEKAREKIEAVGGEVVEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>P54054</accession>
    <name>RL14E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L14e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl14e</name>
        <name type="ordered locus">MJ0657</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64382">
        <property type="entry name" value="A64382"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870162.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54054"/>
    <dbReference type="SMR" id="P54054"/>
    <dbReference type="STRING" id="243232.MJ_0657"/>
    <dbReference type="EnsemblBacteria" id="AAB98652">
        <property type="protein sequence ID" value="AAB98652"/>
        <property type="gene ID" value="MJ_0657"/>
    </dbReference>
    <dbReference type="GeneID" id="24891860"/>
    <dbReference type="KEGG" id="mja:MJ_0657"/>
    <dbReference type="eggNOG" id="arCOG04167">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_183474_0_0_2"/>
    <dbReference type="InParanoid" id="P54054"/>
    <dbReference type="OMA" id="RRCNIKH"/>
    <dbReference type="OrthoDB" id="118643at2157"/>
    <dbReference type="PhylomeDB" id="P54054"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06088">
        <property type="entry name" value="KOW_RPL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00721">
        <property type="entry name" value="Ribosomal_L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_L2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023651">
        <property type="entry name" value="Ribosomal_L14e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_protein_L14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041985">
        <property type="entry name" value="RPL14_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="PTHR11127"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="SSF50104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000132046" description="50S ribosomal protein L14e">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00721"/>
        </source>
    </evidence>
    <sequence length="80" mass="8739" checksum="F3B78C1E42629C93" modified="1996-10-01" version="1">MPAIEVGRVCIKTAGREAGKVCVIVDILDKNFVIVDGLVKRRRCNIKHLEPTEKKVDIPKGASTEEVKLALDAAGLLKEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>P81303</accession>
    <name>NOP10_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">nop10</name>
        <name type="ordered locus">MJ0116.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="interaction">
        <interactant intactId="EBI-9026465">
            <id>P81303</id>
        </interactant>
        <interactant intactId="EBI-9026474">
            <id>Q57612</id>
            <label>truB</label>
        </interactant>
        <organismsDiffer>false</organismsDiffer>
        <experiments>5</experiments>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PDB" id="2APO">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.95 A"/>
        <property type="chains" value="B=1-60"/>
    </dbReference>
    <dbReference type="PDB" id="2AQC">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-60"/>
    </dbReference>
    <dbReference type="PDBsum" id="2APO"/>
    <dbReference type="PDBsum" id="2AQC"/>
    <dbReference type="AlphaFoldDB" id="P81303"/>
    <dbReference type="BMRB" id="P81303"/>
    <dbReference type="SMR" id="P81303"/>
    <dbReference type="DIP" id="DIP-60500N"/>
    <dbReference type="IntAct" id="P81303">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0116.1"/>
    <dbReference type="PRIDE" id="P81303"/>
    <dbReference type="EnsemblBacteria" id="AAB98109">
        <property type="protein sequence ID" value="AAB98109"/>
        <property type="gene ID" value="MJ_0116.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0116.1"/>
    <dbReference type="eggNOG" id="arCOG00906">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196480_1_0_2"/>
    <dbReference type="InParanoid" id="P81303"/>
    <dbReference type="OMA" id="FRIRKCP"/>
    <dbReference type="PhylomeDB" id="P81303"/>
    <dbReference type="EvolutionaryTrace" id="P81303"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="SSF144210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0690">Ribosome biogenesis</keyword>
    <keyword id="KW-0698">rRNA processing</keyword>
    <feature type="chain" id="PRO_0000149018" description="Ribosome biogenesis protein Nop10">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="15"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="43"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2APO"/>
        </source>
    </evidence>
    <sequence length="60" mass="7130" checksum="F803BDAF7F216DE7" modified="1998-07-15" version="1">MVEMRMKKCPKCGLYTLKEICPKCGEKTVIPKPPKFSLEDRWGKYRRMLKRALKNKNKAE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>P81308</accession>
    <name>Y45A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0458.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0458.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81308"/>
    <dbReference type="SMR" id="P81308"/>
    <dbReference type="STRING" id="243232.MJ_0458.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98468">
        <property type="protein sequence ID" value="AAB98468"/>
        <property type="gene ID" value="MJ_0458.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0458.1"/>
    <dbReference type="eggNOG" id="arCOG01989">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_196471_1_0_2"/>
    <dbReference type="InParanoid" id="P81308"/>
    <dbReference type="OMA" id="KCGFEGP"/>
    <dbReference type="PhylomeDB" id="P81308"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044720">
        <property type="entry name" value="HVO_2753-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011668">
        <property type="entry name" value="HVO_2753-like_ZBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733">
        <property type="entry name" value="PTHR40733"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07754">
        <property type="entry name" value="HVO_2753_ZBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106887" description="Uncharacterized protein MJ0458.1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <sequence length="57" mass="6459" checksum="0FB4D7019B6BC426" modified="1998-07-15" version="1">MGEMKYVCISCNAEIAPREKSTKFPCPNCGEVEIVRCERCRKLNNPYKCPKCGFEGP</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>P81318</accession>
    <name>YC8A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1282.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1282.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0902.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870796.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81318"/>
    <dbReference type="STRING" id="243232.MJ_1282.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99298">
        <property type="protein sequence ID" value="AAB99298"/>
        <property type="gene ID" value="MJ_1282.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1452181"/>
    <dbReference type="KEGG" id="mja:MJ_1282.1"/>
    <dbReference type="eggNOG" id="arCOG02298">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_999708_0_0_2"/>
    <dbReference type="OMA" id="GDCKFLM"/>
    <dbReference type="OrthoDB" id="87374at2157"/>
    <dbReference type="PhylomeDB" id="P81318"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107250" description="Uncharacterized protein MJ1282.1">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="254" mass="29965" checksum="8DDFA786B5D62919" modified="1998-07-15" version="1">MDLGYLYGLICSIYGAVEDWRKREVTDFLWISMLWVGVFIHLLYNKSLLLFFIEIFAVLFITLSVRYEKFNKLVYIGVFLFLLSFILFKSYFALSFLVFYLIGIFLYYLNFMGGGDCKFLMGLSYLKGMFFTFIIFLNAILFVIPYCIFILLINLKNGNHKRLKLKNLPLLFIALKKDIDKVKKFETIMGDDENLSLIPNINEEKEEKKTYKGKVWVTPQLPFLVFICLSYILYIVSPFPLIFKVIELVIKSHF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>P81325</accession>
    <name>YF9A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1597.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1597.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81325"/>
    <dbReference type="STRING" id="243232.MJ_1597.1"/>
    <dbReference type="EnsemblBacteria" id="AAB99628">
        <property type="protein sequence ID" value="AAB99628"/>
        <property type="gene ID" value="MJ_1597.1"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1597.1"/>
    <dbReference type="HOGENOM" id="CLU_1954679_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107432" description="Uncharacterized protein MJ1597.1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <sequence length="128" mass="15255" checksum="AA298F1AE774032D" modified="1998-07-15" version="1">MFPFRIGLILTTKEEYEVLNGLFQFPYRIGLILIAKILVWHWRVYLFSFPSSKRSDFNFLKQPQVKHWFISFPSSKRSDFNLLCYCQFPYSPPKNFHPPRGLILTENEVKELKKCLCFHPPRGLILTV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-21" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>P81332</accession>
    <name>ACDE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit epsilon</fullName>
            <shortName evidence="1">ACDS complex subunit epsilon</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ACDS complex carbon monoxide dehydrogenase subunit epsilon</fullName>
            <shortName evidence="1">ACDS CODH subunit epsilon</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhB</name>
        <name type="ordered locus">MJ0154</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase. The precise role of the epsilon subunit is unclear; it may have a stabilizing role within the alpha(2)epsilon(2) component and/or be involved in electron transfer to FAD during a potential FAD-mediated CO oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdhB family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869649.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81332"/>
    <dbReference type="SMR" id="P81332"/>
    <dbReference type="STRING" id="243232.MJ_0154"/>
    <dbReference type="EnsemblBacteria" id="AAB98136">
        <property type="protein sequence ID" value="AAB98136"/>
        <property type="gene ID" value="MJ_0154"/>
    </dbReference>
    <dbReference type="GeneID" id="1450998"/>
    <dbReference type="KEGG" id="mja:MJ_0154"/>
    <dbReference type="eggNOG" id="arCOG04408">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1773184_0_0_2"/>
    <dbReference type="InParanoid" id="P81332"/>
    <dbReference type="OMA" id="ITYYYLA"/>
    <dbReference type="OrthoDB" id="109783at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019385">
        <property type="term" value="P:methanogenesis, from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01134">
        <property type="entry name" value="CdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003704">
        <property type="entry name" value="CO_DH_CoA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02552">
        <property type="entry name" value="CO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006035">
        <property type="entry name" value="CO_dh_b_ACDS_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00315">
        <property type="entry name" value="cdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000155090" description="Acetyl-CoA decarbonylase/synthase complex subunit epsilon">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01134"/>
        </source>
    </evidence>
    <sequence length="146" mass="16858" checksum="EFF8B65DDF4518D6" modified="1998-06-01" version="1">MDERFIAYIPTAGSNVAHAEITSPTLVKMMIRRAKKPILILGENLEENEKELISKLIEKFNLKTIKTPEEMNLMAIMKYLASSDYDLALFTGITYYYLAQAATHLKQFSNVVTISIDKYYQPNTLYSFPNLSKEEYLDYLRKLLEG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57561</accession>
    <name>Y096_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0096</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0096</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64311">
        <property type="entry name" value="H64311"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57561"/>
    <dbReference type="STRING" id="243232.MJ_0096"/>
    <dbReference type="EnsemblBacteria" id="AAB98087">
        <property type="protein sequence ID" value="AAB98087"/>
        <property type="gene ID" value="MJ_0096"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0096"/>
    <dbReference type="eggNOG" id="arCOG05021">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1131620_0_0_2"/>
    <dbReference type="OMA" id="IWYSYFI"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106692" description="Uncharacterized protein MJ0096">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="262" mass="29463" checksum="47329C0221DDCFA2" modified="1997-11-01" version="1">MEVKAMEIFKKYLSLNIPKKILITYFLCWAGFLFSFSVGKFLLYLSSILKSNFISEPAKLAQSVGTAKFNAVSSAVSNTVGVKNAYLTYALSYIVSNFMGCLIIMFALGALAYLYKKDLEKAKTLEEKEELFKCYQKYLLILFIFTVINPLTGLIGVNLQYSDLIAVLPHGFFEFFGFATAVVVGVELSNKILPIVKREITSKKIVILIACSFIFIFIAGMLEPIDWFIYSYAKAYGIPLLAAFATGYKNLFLYLISMLFKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q57572</accession>
    <name>KPYK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Pyruvate kinase</fullName>
            <shortName>PK</shortName>
            <ecNumber>2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0108</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + pyruvate = ADP + H(+) + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64313">
        <property type="entry name" value="D64313"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57572"/>
    <dbReference type="SMR" id="Q57572"/>
    <dbReference type="STRING" id="243232.MJ_0108"/>
    <dbReference type="EnsemblBacteria" id="AAB98090">
        <property type="protein sequence ID" value="AAB98090"/>
        <property type="gene ID" value="MJ_0108"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0108"/>
    <dbReference type="eggNOG" id="arCOG04120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015439_8_0_2"/>
    <dbReference type="InParanoid" id="Q57572"/>
    <dbReference type="OMA" id="QVPIVQK"/>
    <dbReference type="PhylomeDB" id="Q57572"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_Kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PTHR11817"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="SSF50800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="SSF51621"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="SSF52935"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0670">Pyruvate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000112128" description="Pyruvate kinase">
        <location>
            <begin position="1"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="35"/>
            <end position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P14618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="447" mass="50490" checksum="EA6C4DC031B8C1A4" modified="1997-11-01" version="1">MVGMMRKTKILVTLGPSLENKLDKAINLIDGVRFNMSHATTDYCEKFLNILEKNNIAKVMDLKGIKIRIKEVKLKNKILKMGEKVVIGEDIKLNYNIDTIEEGHFILINDGKIKLRVVEKTDKIIAVVEVGGEIKEGMGVNLPDTRIELPIIDETDLKNIKFAVEKDFEYIALSFVRNKEDVKELKDIISEYKGDCEVISKIETKEGLKNIKEIARESDGVMVARGDLGVEVPIENIPIEQKNILRIANRYGILSITATQILDSMINNPFPTRAEVTDIANAIYDGTDCLMLSNETTIGKYPIEAIKVLNKVAKVADEHYEEFGDRVCLEVESIDEGLVYAVYELYKKLNTKLVITPTYSGRTAKLISKLRINSKIIAPTPNIRTLKRLRLVWGVESCLMEEFDDMEKIINTCREMAKKEIGKGIYLITLGHPIGQKKTNTIKVESI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="148" xmlns="http://uniprot.org/uniprot">
    <accession>Q57573</accession>
    <name>BSUHB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Fructose-1,6-bisphosphatase/inositol-1-monophosphatase</fullName>
            <shortName>FBPase/IMPase</shortName>
            <ecNumber evidence="3">3.1.3.11</ecNumber>
            <ecNumber evidence="1 3">3.1.3.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Inositol-1-phosphatase</fullName>
            <shortName>I-1-Pase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">suhB</name>
        <name type="ordered locus">MJ0109</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1998" name="Appl. Environ. Microbiol." volume="64" first="2609" last="2615">
            <title>Cloning and expression of the inositol monophosphatase gene from Methanococcus jannaschii and characterization of the enzyme.</title>
            <authorList>
                <person name="Chen L."/>
                <person name="Roberts M.F."/>
            </authorList>
            <dbReference type="PubMed" id="9647837"/>
        </citation>
        <scope>FUNCTION AS IMPASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>ACTIVITY REGULATION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2000" name="Nat. Struct. Biol." volume="7" first="1046" last="1050">
            <title>MJ0109 is an enzyme that is both an inositol monophosphatase and the 'missing' archaeal fructose-1,6-bisphosphatase.</title>
            <authorList>
                <person name="Stec B."/>
                <person name="Yang H."/>
                <person name="Johnson K.A."/>
                <person name="Chen L."/>
                <person name="Roberts M.F."/>
            </authorList>
            <dbReference type="PubMed" id="11062561"/>
            <dbReference type="DOI" id="10.1038/80968"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) IN COMPLEX WITH PHOSPHATE AND METAL IONS</scope>
        <scope>FUNCTION AS BOTH FBPASE AND IMPASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>KINETIC PARAMETERS</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2001" name="Biochemistry" volume="40" first="618" last="630">
            <title>Crystal structure and catalytic mechanism of the MJ0109 gene product: a bifunctional enzyme with inositol monophosphatase and fructose 1,6-bisphosphatase activities.</title>
            <authorList>
                <person name="Johnson K.A."/>
                <person name="Chen L."/>
                <person name="Yang H."/>
                <person name="Roberts M.F."/>
                <person name="Stec B."/>
            </authorList>
            <dbReference type="PubMed" id="11170378"/>
            <dbReference type="DOI" id="10.1021/bi0016422"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEXES WITH MYO-INOSITOL PHOSPHATE; MYO-INOSITOL AND METAL IONS</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 3">Phosphatase with broad specificity; it can dephosphorylate fructose 1,6-bisphosphate, both D and L isomers of inositol-1-phosphate (I-1-P), 2'-AMP, pNPP, beta-glycerol phosphate, and alpha-D-glucose-1-phosphate. Cannot hydrolyze glucose-6-phosphate, fructose-6-phosphate, NAD(+) or 5'-AMP. May be involved in the biosynthesis of a unique osmolyte, di-myo-inositol 1,1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 3">
            <text>a myo-inositol phosphate + H2O = myo-inositol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24056"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:84139"/>
            <dbReference type="EC" id="3.1.3.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">IMPase activity is inhibited by Ca(2+) and Zn(2+). In contrast to mammalian I-1-P phosphatases, is not inhibited by Li(+) up to 100 mM.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="1 3">0.091 mM for inositol-1-phosphate (at 85 degrees Celsius)</KM>
            <KM evidence="1 3">0.038 mM for D-fructose 1,6-bisphosphate (at 85 degrees Celsius)</KM>
            <Vmax evidence="1 3">9.3 umol/min/mg enzyme for IMPase activity (at 85 degrees Celsius)</Vmax>
            <text>kcat is 4.2 sec(-1) for IMPase activity (at 85 degrees Celsius) and 7.0 sec(-1) for FBPase activity (at 85 degrees Celsius).</text>
        </kinetics>
        <temperatureDependence>
            <text evidence="3">Is thermostable. After incubation at 85 degrees Celsius for 30 minutes, more than 95% of I-1-Pase activity remains.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1 3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the inositol monophosphatase superfamily. FBPase class 4 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="3"/>
    <dbReference type="EC" id="3.1.3.25" evidence="1 3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64313">
        <property type="entry name" value="E64313"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869601.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1DK4">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="A/B=1-252"/>
    </dbReference>
    <dbReference type="PDB" id="1G0H">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
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    <keyword id="KW-1185">Reference proteome</keyword>
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    </evidence>
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    <sequence length="252" mass="28578" checksum="3ED5B4401075EE6E" modified="1996-11-01" version="1">MKWDEIGKNIAKEIEKEILPYFGRKDKSYVVGTSPSGDETEIFDKISEDIALKYLKSLNVNIVSEELGVIDNSSEWTVVIDPIDGSFNFINGIPFFAFCFGVFKNNEPYYGLTYEFLTKSFYEAYKGKGAYLNGRKIKVKDFNPNNIVISYYPSKKIDLEKLRNKVKRVRIFGAFGLEMCYVAKGTLDAVFDVRPKVRAVDIASSYIICKEAGALITDENGDELKFDLNATDRLNIIVANSKEMLDIILDLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="131" xmlns="http://uniprot.org/uniprot">
    <accession>Q57635</accession>
    <name>DNLI_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA ligase</fullName>
            <ecNumber evidence="1">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">lig</name>
        <name type="ordered locus">MJ0171</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB98156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="D64321"/>
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    <dbReference type="RefSeq" id="WP_010869666.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="entry name" value="DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022865">
        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="SSF117018"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0233">DNA recombination</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000059604" description="DNA ligase">
        <location>
            <begin position="1"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="1">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="432"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="438"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <sequence length="573" mass="66790" checksum="6F95434EE968038C" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q57666</accession>
    <name>Y213_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0213</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0213</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64326">
        <property type="entry name" value="F64326"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869709.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57666"/>
    <dbReference type="SMR" id="Q57666"/>
    <dbReference type="STRING" id="243232.MJ_0213"/>
    <dbReference type="EnsemblBacteria" id="AAB98204">
        <property type="protein sequence ID" value="AAB98204"/>
        <property type="gene ID" value="MJ_0213"/>
    </dbReference>
    <dbReference type="GeneID" id="1451063"/>
    <dbReference type="KEGG" id="mja:MJ_0213"/>
    <dbReference type="eggNOG" id="arCOG01042">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_131306_1_1_2"/>
    <dbReference type="InParanoid" id="Q57666"/>
    <dbReference type="OMA" id="RTFCYAT"/>
    <dbReference type="OrthoDB" id="104786at2157"/>
    <dbReference type="PhylomeDB" id="Q57666"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_L5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="UPF0201"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="SSF55282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106744" description="Uncharacterized protein MJ0213">
        <location>
            <begin position="1"/>
            <end position="149"/>
        </location>
    </feature>
    <sequence length="149" mass="17269" checksum="21286C2F1E190CCD" modified="1996-11-01" version="1">MLNSIKLSAIVHATEDEDKVLEAIEFFIPENVDEEKIDLDVVETQGYFGNPIKIINVNVEGKEAKKIFKHIIDLIKSDDKNINKLKKDLHLRVEDNKFYVRFDKQKAYLGECRVVDGDDIIRAVFNFKIFTPKNKEEKVKEIVANELGF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q57714</accession>
    <name>PORB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Pyruvate synthase subunit PorB</fullName>
            <ecNumber>1.2.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Pyruvate oxidoreductase beta chain</fullName>
            <shortName>POR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Pyruvic-ferredoxin oxidoreductase subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">porB</name>
        <name type="ordered locus">MJ0266</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>CoA + 2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate = acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="2">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64333">
        <property type="entry name" value="C64333"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57714"/>
    <dbReference type="SMR" id="Q57714"/>
    <dbReference type="STRING" id="243232.MJ_0266"/>
    <dbReference type="EnsemblBacteria" id="AAB98253">
        <property type="protein sequence ID" value="AAB98253"/>
        <property type="gene ID" value="MJ_0266"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0266"/>
    <dbReference type="eggNOG" id="arCOG01601">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058423_0_0_2"/>
    <dbReference type="InParanoid" id="Q57714"/>
    <dbReference type="OMA" id="ATGCMEV"/>
    <dbReference type="PhylomeDB" id="Q57714"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000099903" description="Pyruvate synthase subunit PorB">
        <location>
            <begin position="1"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P94692"/>
        </source>
    </evidence>
    <sequence length="298" mass="33098" checksum="D772C50EE8611A49" modified="2000-05-30" version="3">MIVMQFPREEYFAPGHRGCAGCGAAIVARLLLKVAGKDTIITNATGCLEVMTTPYPETSWRVPWIHTAFENAAATASGIEAAVKALKRKRGKFADKKINVIAIGGDGGTADIGFQALSGAMERGHDILYIMYDNEAYMNTGIQRSSSTPFMAATTTSPAGSKIRGEDRPKKDMTMIMAAHGIPYVATACISYPEDFMRKVKKALSIEGPKFIQVLQPCTTGWGYPPEKTIEIGRLAVETGIFPLYEIENGEFRITYKPAKRKPVREYLKMQKRYRHLTDEDIERIQKYIDEKCKLLGL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57744</accession>
    <name>Y296_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable metallo-hydrolase MJ0296</fullName>
            <ecNumber>3.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0296</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-beta-lactamase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64337">
        <property type="entry name" value="A64337"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57744"/>
    <dbReference type="SMR" id="Q57744"/>
    <dbReference type="STRING" id="243232.MJ_0296"/>
    <dbReference type="EnsemblBacteria" id="AAB98284">
        <property type="protein sequence ID" value="AAB98284"/>
        <property type="gene ID" value="MJ_0296"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0296"/>
    <dbReference type="eggNOG" id="arCOG00504">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_2_6_2"/>
    <dbReference type="InParanoid" id="Q57744"/>
    <dbReference type="OMA" id="WTPGHTF"/>
    <dbReference type="PhylomeDB" id="Q57744"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000106779" description="Probable metallo-hydrolase MJ0296">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="203" mass="23236" checksum="9039A9CB6DF9E75D" modified="1996-11-01" version="1">MIYISMFKFLFKILIGEIMIKLLYEGILIRENGIIKKASSSSTLIITDNNNIIVDTSTKDMENIIIKGLSELNLSPNDIDVVINTHLHYDHIENNPIFKNATFYASPKEFGFNDNFEDFKKFKDKEIEIIETPGHTYGSISVIYKDYVVVGDASPLKNNILKMIPPKLNVDEKLALESLKKIRKLRKNVITGHEGIVYKEKLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q57747</accession>
    <name>FBPAP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="1">FBP A/P</shortName>
            <shortName evidence="1">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="1">3.1.3.11</ecNumber>
            <ecNumber evidence="1">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">fbp</name>
        <name type="ordered locus">MJ0299</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="1"/>
    <dbReference type="EC" id="4.1.2.13" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64337">
        <property type="entry name" value="D64337"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57747"/>
    <dbReference type="SMR" id="Q57747"/>
    <dbReference type="STRING" id="243232.MJ_0299"/>
    <dbReference type="EnsemblBacteria" id="AAB98286">
        <property type="protein sequence ID" value="AAB98286"/>
        <property type="gene ID" value="MJ_0299"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0299"/>
    <dbReference type="eggNOG" id="arCOG04180">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041630_0_0_2"/>
    <dbReference type="InParanoid" id="Q57747"/>
    <dbReference type="OMA" id="YMRRHGP"/>
    <dbReference type="PhylomeDB" id="Q57747"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="PTHR38341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="SSF111249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0704">Schiff base</keyword>
    <feature type="chain" id="PRO_0000106782" description="Fructose-1,6-bisphosphate aldolase/phosphatase">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="1">
        <location>
            <position position="17"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="1">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="1">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="237"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="246"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="1">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <sequence length="389" mass="43246" checksum="1BB8D890F931CE73" modified="1996-11-01" version="1">MSRMENNKVTISVIKADVGGLCGHTLAPDELLEACEAVLEEAVDEIILDYYVTRCGDDIDLIMSHKLGCDNEKVHGLAWRAFEEATKVAKELKLYGAGQDLLADSFSGNVRGMGPGCAEMEFVERKSEPIVVFCCDKTDPTAFNYPLFKMFADPFNTAGLVFDPSMISGFKFEVHDVVGHKKVFLDTPEEMYMLLALIGDYEKYAIKRVYRRRDNEIAAVVSTEKLNYIAGEYVGKDDPVAIVRAQSGFPAVGEVLEPFANPHFVPGWMRGSHWGPLMPVGEEDATPTRFDGPARIIALGFQVCDGMLIGPNDLFADKGFDKAREKALEMADIIRRMGPFQPHRLPATMMEYTTVPKVLEALEDRFIPLEGLELIEEGGITRKDRGDVE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q57751</accession>
    <name>RISB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName>DMRL synthase</shortName>
            <shortName>LS</shortName>
            <shortName>Lumazine synthase</shortName>
            <ecNumber>2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">ribH</name>
        <name type="ordered locus">MJ0303</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Eur. J. Biochem." volume="270" first="1025" last="1032">
            <title>Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii.</title>
            <authorList>
                <person name="Haase I."/>
                <person name="Mortl S."/>
                <person name="Kohler P."/>
                <person name="Bacher A."/>
                <person name="Fischer M."/>
            </authorList>
            <dbReference type="PubMed" id="12603336"/>
            <dbReference type="DOI" id="10.1046/j.1432-1033.2003.03478.x"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-10</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>SUBUNIT</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">12.5 uM for 5-amino-6-(D-ribitylamino)uracil (at 37 degrees Celsius and pH 7.0)</KM>
            <KM evidence="3">52 uM for 3,4-dihydroxy-2-butanone 4-phosphate (at 37 degrees Celsius and pH 7.0)</KM>
            <Vmax evidence="3">11.0 nmol/min/mg enzyme (at 37 degrees Celsius and pH 7.0)</Vmax>
            <Vmax evidence="3">90.0 nmol/min/mg enzyme (at 70 degrees Celsius and pH 7.0)</Vmax>
        </kinetics>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.</text>
    </comment>
    <comment type="mass spectrometry" mass="15645.0" method="Electrospray" evidence="3"/>
    <comment type="similarity">
        <text evidence="4">Belongs to the DMRL synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64337">
        <property type="entry name" value="H64337"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57751"/>
    <dbReference type="SMR" id="Q57751"/>
    <dbReference type="STRING" id="243232.MJ_0303"/>
    <dbReference type="EnsemblBacteria" id="AAB98290">
        <property type="protein sequence ID" value="AAB98290"/>
        <property type="gene ID" value="MJ_0303"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0303"/>
    <dbReference type="eggNOG" id="arCOG01323">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089358_3_1_2"/>
    <dbReference type="InParanoid" id="Q57751"/>
    <dbReference type="OMA" id="SGPGMTR"/>
    <dbReference type="PhylomeDB" id="Q57751"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14604"/>
    <dbReference type="BRENDA" id="2.5.1.78">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q57751"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="PTHR21058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="SSF52121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000134843" description="6,7-dimethyl-8-ribityllumazine synthase">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="70"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12603336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="12603336"/>
        </source>
    </evidence>
    <sequence length="141" mass="15645" checksum="646C2C494E27F371" modified="1996-11-01" version="1">MVLMVNLGFVIAEFNRDITYMMEKVAEEHAEFLGATVKYKIVVPGVFDMPLAVKKLLEKDDVDAVVTIGCVIEGETEHDEIVVHNAARKIADLALQYDKPVTLGISGPGMTRLQAQERVDYGKRAVEAAVKMVKRLKALEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q57783</accession>
    <name>Y337_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0337</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0337</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64342">
        <property type="entry name" value="A64342"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57783"/>
    <dbReference type="STRING" id="243232.MJ_0337"/>
    <dbReference type="PRIDE" id="Q57783"/>
    <dbReference type="EnsemblBacteria" id="AAB98325">
        <property type="protein sequence ID" value="AAB98325"/>
        <property type="gene ID" value="MJ_0337"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0337"/>
    <dbReference type="eggNOG" id="arCOG09642">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1060113_0_0_2"/>
    <dbReference type="OMA" id="TIANIYT"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106807" description="Uncharacterized protein MJ0337">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <sequence length="262" mass="29045" checksum="7D2C22E3EBE580E3" modified="1996-11-01" version="1">MMALNEYDAQIVEQAMKPILDSNLFSRKIFQKKKIPEDVEVYNLVQVVFDENAFKKGSMELTEVPIKTTTSPFTVFDINLKVTKARRFVEGPNADYNKAQIFEGLAKVVARAENQYSIDALSKNNTAVGASASWNGADTTPDKIANDIIDAKTKIEQYSNAKCALVAPVDAIACFRKIGTQGFSAYDETKDFIKEIIPTNLITDKSAYLVPIDIAILQMGVAVEADQFIEKKATQVEFIFTEAISPMVKEKNGIIKIQKVLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q57820</accession>
    <name>CAS6A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endoribonuclease Cas6 1</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas6a</name>
        <name type="ordered locus">MJ0375</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). This protein processes pre-crRNA into individual crRNA units.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64346">
        <property type="entry name" value="G64346"/>
    </dbReference>
    <dbReference type="PDB" id="5YI6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B/C/D=1-254"/>
    </dbReference>
    <dbReference type="PDBsum" id="5YI6"/>
    <dbReference type="AlphaFoldDB" id="Q57820"/>
    <dbReference type="SMR" id="Q57820"/>
    <dbReference type="STRING" id="243232.MJ_0375"/>
    <dbReference type="EnsemblBacteria" id="AAB98364">
        <property type="protein sequence ID" value="AAB98364"/>
        <property type="gene ID" value="MJ_0375"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0375"/>
    <dbReference type="eggNOG" id="arCOG04342">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089858_1_1_2"/>
    <dbReference type="InParanoid" id="Q57820"/>
    <dbReference type="OMA" id="FGMVKVD"/>
    <dbReference type="PhylomeDB" id="Q57820"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045747">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="PTHR36984"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005054">
        <property type="entry name" value="PF1131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000106838" description="CRISPR-associated endoribonuclease Cas6 1">
        <location>
            <begin position="1"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="5"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="17"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="21"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="40"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="turn" evidence="3">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="47"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="63"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="126"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="137"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="145"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="182"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="226"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="230"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="237"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8U1S4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="5YI6"/>
        </source>
    </evidence>
    <sequence length="254" mass="30184" checksum="D9BCD0EB681148E5" modified="1996-11-01" version="1">MRESMRIELELQTDNFTVIPYNHQYYLASAIYNKIHSANPAYAKRLHNYQKFKFFTFSLLQIRKRVIRKEGIETIDGKAYLYISSPNNEFIENFVAGLLEDGKLRVGNVEFFVRKAKILPIPKKFNILKTISPIYLKTMIETEDGLKTYDLLPNNSKFYENLKNNLKKKYEAFYNEKCDMNFEFEVLKFRPKRMRIKNDIYCRCSEMVFKVWGDYDLIKFGYECGFGEKNSMGFGMVVNVEDKNQKNKKLKTKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57828</accession>
    <name>CS3HE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated helicase Cas3</fullName>
            <ecNumber>3.6.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cas3</name>
        <name type="synonym">cas3'</name>
        <name type="ordered locus">MJ0383</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="EMBO J." volume="30" first="4616" last="4627">
            <title>Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference.</title>
            <authorList>
                <person name="Beloglazova N."/>
                <person name="Petit P."/>
                <person name="Flick R."/>
                <person name="Brown G."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
            <dbReference type="PubMed" id="22009198"/>
            <dbReference type="DOI" id="10.1038/emboj.2011.377"/>
        </citation>
        <scope>PROBABLE FUNCTION</scope>
        <scope>ATP-DEPENDENCE</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Probably unwinds dsDNA templates, thus providing substrates for CRISPR-associated endonuclease Cas3-HD. Unwinding is strongly increased in the presence of ATP, implying the helicase uses ATP to unwind substrate.</text>
    </comment>
    <comment type="domain">
        <text>Proteins of this family have an N-terminal nuclease domain and a C-terminal helicase/ATPase domain. In some CRISPR/Cas systems the domains are swapped, in others they are encoded separately.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated helicase Cas3 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64347">
        <property type="entry name" value="G64347"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869882.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57828"/>
    <dbReference type="SMR" id="Q57828"/>
    <dbReference type="STRING" id="243232.MJ_0383"/>
    <dbReference type="EnsemblBacteria" id="AAB98371">
        <property type="protein sequence ID" value="AAB98371"/>
        <property type="gene ID" value="MJ_0383"/>
    </dbReference>
    <dbReference type="GeneID" id="1451240"/>
    <dbReference type="KEGG" id="mja:MJ_0383"/>
    <dbReference type="eggNOG" id="arCOG01444">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_502152_0_0_2"/>
    <dbReference type="OMA" id="IQRIGRC"/>
    <dbReference type="OrthoDB" id="18962at2157"/>
    <dbReference type="PhylomeDB" id="Q57828"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006474">
        <property type="entry name" value="Helicase_Cas3_CRISPR-ass_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01587">
        <property type="entry name" value="cas3_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106846" description="CRISPR-associated helicase Cas3">
        <location>
            <begin position="1"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="27"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="244"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="40"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="614" mass="71568" checksum="016CF1A6F4EDEF1A" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q57914</accession>
    <name>Y491_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0491</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0491</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64361">
        <property type="entry name" value="C64361"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57914"/>
    <dbReference type="STRING" id="243232.MJ_0491"/>
    <dbReference type="PRIDE" id="Q57914"/>
    <dbReference type="EnsemblBacteria" id="AAB98482">
        <property type="protein sequence ID" value="AAB98482"/>
        <property type="gene ID" value="MJ_0491"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0491"/>
    <dbReference type="eggNOG" id="arCOG04873">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143947_0_0_2"/>
    <dbReference type="OMA" id="IGCDCDY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014515">
        <property type="entry name" value="UCP921964"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09893">
        <property type="entry name" value="DUF2120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021964">
        <property type="entry name" value="UCP921964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106896" description="Uncharacterized protein MJ0491">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <sequence length="167" mass="19481" checksum="D77A570BA3AB583C" modified="1996-11-01" version="1">MHLENLLKSLKNTVKKLKTYNFGVSMWKVNIGRLMHALNAFKGSKPLFETDEMLMVKGVCRDEEVEKYGSIKDYLVKKLEKEGFEIVEDIKEIDKFVSRINEILKENPLYPDTFGFERMKESFEMIGCECDYVIAKKRNIMVGVCMYFDKKMKNPKFVEVVGVLLPS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="70" xmlns="http://uniprot.org/uniprot">
    <accession>Q57980</accession>
    <name>Y560_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0560</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0560</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64369">
        <property type="entry name" value="H64369"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870064.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57980"/>
    <dbReference type="EnsemblBacteria" id="AAB98559">
        <property type="protein sequence ID" value="AAB98559"/>
        <property type="gene ID" value="MJ_0560"/>
    </dbReference>
    <dbReference type="GeneID" id="1451425"/>
    <dbReference type="KEGG" id="mja:MJ_0560"/>
    <dbReference type="eggNOG" id="arCOG12718">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1850669_0_0_2"/>
    <dbReference type="OMA" id="CIDFKNV"/>
    <dbReference type="OrthoDB" id="136170at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106931" description="Uncharacterized protein MJ0560">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <sequence length="138" mass="16470" checksum="752B2B7E63EA9721" modified="1996-11-01" version="1">MMIMKFVRLEFISYEESYDFEFMAPDDITEDKFIDDLSDAIVKSINWEYIKGYFQEEDELGMEILPNLIDCIDFKNVNVEMEKKGYKPIKYDIIVYAGAWSYFNPKKLSIIDFHETGELTKLEKAIQEKLKRLKTDIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q58001</accession>
    <name>THIRX_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thioredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0581</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Does not function as a glutathione-disulfide oxidoreductase in the presence of glutathione and glutathione reductase (By similarity). Has low thioredoxin activity in vitro.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glutaredoxin family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64372">
        <property type="entry name" value="E64372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58001"/>
    <dbReference type="SMR" id="Q58001"/>
    <dbReference type="STRING" id="243232.MJ_0581"/>
    <dbReference type="EnsemblBacteria" id="AAB98575">
        <property type="protein sequence ID" value="AAB98575"/>
        <property type="gene ID" value="MJ_0581"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0581"/>
    <dbReference type="eggNOG" id="arCOG02713">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_18_1_2"/>
    <dbReference type="InParanoid" id="Q58001"/>
    <dbReference type="OMA" id="KYGVMST"/>
    <dbReference type="PhylomeDB" id="Q58001"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005243">
        <property type="entry name" value="Redox_disulphide_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36450">
        <property type="entry name" value="PTHR36450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037031">
        <property type="entry name" value="Redox_disulphide_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="SSF52833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00412">
        <property type="entry name" value="redox_disulf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1015">Disulfide bond</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0676">Redox-active center</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000141653" description="Thioredoxin">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="2">
        <location>
            <position position="18"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Redox-active" evidence="1">
        <location>
            <begin position="15"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="O26981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P0AA25"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="86" mass="9634" checksum="AA89FE52EC6C0D67" modified="1996-11-01" version="1">MVRVMVVIRIFGTGCPKCNQTYENVKKAVEELGIDAEIVKVTDVNEIAEWVFVTPGVAFDDVIVFEGKIPSVEEIKEELKSYLEGK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58003</accession>
    <name>Y583_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0583</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0583</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64372">
        <property type="entry name" value="G64372"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58003"/>
    <dbReference type="SMR" id="Q58003"/>
    <dbReference type="STRING" id="243232.MJ_0583"/>
    <dbReference type="EnsemblBacteria" id="AAB98582">
        <property type="protein sequence ID" value="AAB98582"/>
        <property type="gene ID" value="MJ_0583"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0583"/>
    <dbReference type="eggNOG" id="arCOG05041">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_200897_0_0_2"/>
    <dbReference type="OMA" id="EVDVCPC"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106943" description="Uncharacterized protein MJ0583">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <sequence length="57" mass="6626" checksum="E643AD862336DDA8" modified="1996-11-01" version="1">MIVWNLICPKCGKRLRYKVDVCPCMASEVELPNCPDCGEKMVHDYTSLKGRRRIRRG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58019</accession>
    <name>Y602_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0602</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0602</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64375">
        <property type="entry name" value="B64375"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870106.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58019"/>
    <dbReference type="SMR" id="Q58019"/>
    <dbReference type="STRING" id="243232.MJ_0602"/>
    <dbReference type="PRIDE" id="Q58019"/>
    <dbReference type="EnsemblBacteria" id="AAB98598">
        <property type="protein sequence ID" value="AAB98598"/>
        <property type="gene ID" value="MJ_0602"/>
    </dbReference>
    <dbReference type="GeneID" id="1451467"/>
    <dbReference type="KEGG" id="mja:MJ_0602"/>
    <dbReference type="eggNOG" id="arCOG08295">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1064006_0_0_2"/>
    <dbReference type="OMA" id="KPYKSYE"/>
    <dbReference type="OrthoDB" id="61088at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106952" description="Uncharacterized protein MJ0602">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <sequence length="261" mass="30835" checksum="81E4A011C761E8F4" modified="1996-11-01" version="1">MLLEKSKIEIIEQFIHILEILEMYAKEGSDEKAIIRLMLDYLEKGYVLDDDILPIASKISEIAKKVGSFDMKREISLLLFGERKRLTKSQKNKIKKIIEILEYLKSYIEKKPYKSYEDKLILNLIGLKILRLDNGIVLDYNSEVRSLTNMALRVGSYELKNEIELLLTGRRRRKLTEEFIQKRLSIIKILEMVKDFIDKKEFKSSFDYEALFLINLKIYRIEEGILKNFDEEIESILNIARKVGNHKLREQIVLLKESIKN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="85" xmlns="http://uniprot.org/uniprot">
    <accession>Q58022</accession>
    <name>Y605_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative toxin MJ0605</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>UPF0332 protein MJ0605</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0605</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Putative toxin component of a putative type VII toxin-antitoxin (TA) system. Its cognate antitoxin might be MJ0604.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0332 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64375">
        <property type="entry name" value="E64375"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870109.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58022"/>
    <dbReference type="SMR" id="Q58022"/>
    <dbReference type="STRING" id="243232.MJ_0605"/>
    <dbReference type="EnsemblBacteria" id="AAB98599">
        <property type="protein sequence ID" value="AAB98599"/>
        <property type="gene ID" value="MJ_0605"/>
    </dbReference>
    <dbReference type="GeneID" id="1451470"/>
    <dbReference type="KEGG" id="mja:MJ_0605"/>
    <dbReference type="eggNOG" id="arCOG02123">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_151247_1_0_2"/>
    <dbReference type="InParanoid" id="Q58022"/>
    <dbReference type="OMA" id="YYAMFYA"/>
    <dbReference type="OrthoDB" id="118985at2157"/>
    <dbReference type="PhylomeDB" id="Q58022"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000159640" description="Putative toxin MJ0605">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="134" mass="15596" checksum="4C1D5D5288D3452C" modified="1997-11-01" version="1">MKALLNKDIREEIQALIEIAEENLSAAKILFENKLYRDAVARAYYAIFHSAKALLLTKNLNPKKHAGVIKMFGLYFVNEGYIEEIYGRIITKSYNLRWKADYTTDKPTEEEAESIIYEAEMFVDRIKKALKEIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58039</accession>
    <name>FTSZ2_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cell division protein FtsZ 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">ftsZ2</name>
        <name type="ordered locus">MJ0622</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="1">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the FtsZ family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64377">
        <property type="entry name" value="F64377"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58039"/>
    <dbReference type="SMR" id="Q58039"/>
    <dbReference type="STRING" id="243232.MJ_0622"/>
    <dbReference type="EnsemblBacteria" id="AAB98617">
        <property type="protein sequence ID" value="AAB98617"/>
        <property type="gene ID" value="MJ_0622"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0622"/>
    <dbReference type="eggNOG" id="arCOG02201">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024865_0_1_2"/>
    <dbReference type="InParanoid" id="Q58039"/>
    <dbReference type="OMA" id="IMNQGGV"/>
    <dbReference type="PhylomeDB" id="Q58039"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000917">
        <property type="term" value="P:division septum assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="PTHR30314"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="SSF52490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="SSF55307"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0132">Cell division</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0717">Septation</keyword>
    <feature type="chain" id="PRO_0000114402" description="Cell division protein FtsZ 2">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="47"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="134"/>
            <end position="136"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <sequence length="380" mass="40658" checksum="2A3AD01CCB54FB8C" modified="2000-12-01" version="2">MIYKFNIRNLRMIIMKLVKDALSRSDTTRYLKDEFGEARIVVVGCGGAGNNTINRLMEIGIQGAETIAINTDKQHLEVIQADKKILIGATLTRGLGAGGYPEIGRKAAEMAKNILEEQLKGADLVFVTAGMGGGTGTGSAPVVAEVAKENGAIVVGVVTYPFKIERARMKKADEGIARMSEVCDTVIIIDNNKLLDLVPNLPINDAFKVADEIIAQAVKGITETIAVPSLINIDFADVKAVMSGGGVAMIGVGEVDSSDRGDRVQNVVRETLSCPLLDVDYRGAKGALIHITGGPDLTLKEANDIGEGITKELDPEANVIWGARIDPEMEGCIRVMAIITGVKSPNIVGKDTKPKRIIPKVSKEQSQRKERKIGGIDFIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="102" xmlns="http://uniprot.org/uniprot">
    <accession>Q58049</accession>
    <name>Y632_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0632</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0632</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64378">
        <property type="entry name" value="H64378"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870137.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58049"/>
    <dbReference type="SMR" id="Q58049"/>
    <dbReference type="STRING" id="243232.MJ_0632"/>
    <dbReference type="DNASU" id="1451498"/>
    <dbReference type="EnsemblBacteria" id="AAB98624">
        <property type="protein sequence ID" value="AAB98624"/>
        <property type="gene ID" value="MJ_0632"/>
    </dbReference>
    <dbReference type="GeneID" id="1451498"/>
    <dbReference type="KEGG" id="mja:MJ_0632"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q58049"/>
    <dbReference type="OMA" id="VINEMKY"/>
    <dbReference type="OrthoDB" id="50712at2157"/>
    <dbReference type="PhylomeDB" id="Q58049"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184670" description="Uncharacterized ATP-binding protein MJ0632">
        <location>
            <begin position="1"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="363" mass="43204" checksum="FEB47501ABE63810" modified="1997-11-01" version="1">MKFFNREKEIEEILHIIESEPQRINFIFGSINSGKTALINEIINNRLNKDKYVVFYFDLREIFISKYDDFIEVLFEEYEEPFIDKVKRLFLSLIKDYPDVIKSYALLNITGVVDSIPIPKNTLNELLKKRNVKNVFRYITSVLIKIKREGKQPIIIIDELQKIGDLKLNGFLIYELFNYFVSLTKHKHLCHVFCLSSDSLFIERVYNEAMLEGRCKYILVDDFDRETALKFMDFLAKENNINLTNEDKELIYNYVGGKPIDIIYVINEMKYKKLEDILTSMLKEETQKLKYFLENVKEEDEELYKKVVDALKIFKDSYEIEDIKIPKKLREFLVKKNILFLNPIEGTLKPQSFLVWNAIKRIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58055</accession>
    <name>Y638_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0638</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0638</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64379">
        <property type="entry name" value="F64379"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870143.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58055"/>
    <dbReference type="STRING" id="243232.MJ_0638"/>
    <dbReference type="EnsemblBacteria" id="AAB98634">
        <property type="protein sequence ID" value="AAB98634"/>
        <property type="gene ID" value="MJ_0638"/>
    </dbReference>
    <dbReference type="GeneID" id="1451504"/>
    <dbReference type="KEGG" id="mja:MJ_0638"/>
    <dbReference type="eggNOG" id="arCOG01789">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_084119_0_0_2"/>
    <dbReference type="InParanoid" id="Q58055"/>
    <dbReference type="OMA" id="CINVLEH"/>
    <dbReference type="OrthoDB" id="45753at2157"/>
    <dbReference type="PhylomeDB" id="Q58055"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106965" description="Uncharacterized protein MJ0638">
        <location>
            <begin position="1"/>
            <end position="225"/>
        </location>
    </feature>
    <sequence length="225" mass="26695" checksum="672B812CA1E93E8A" modified="1996-11-01" version="1">MKEVKDFYDKWEPEDFPNYIKLLMNFADELIFEEISLLLKKFENKKDFLVLDCGCGFGAFYNLTKDFNTIYLDISLNLLKRFKLKERKICANILHLPFKDNTFDLVLCINVLEHVNYLKALNEIRRILKNKGKLIVVVVNKDSLIKEEIFNDFKIFHKPLSIKDFEIDGFKIVYSNSVYFLPSIFKISPPIILSKIIEYWKPVDKKLSKIFKNKGQFLIIEMVKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="78" xmlns="http://uniprot.org/uniprot">
    <accession>Q58063</accession>
    <name>Y647_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0647</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0647</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64380">
        <property type="entry name" value="G64380"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58063"/>
    <dbReference type="SMR" id="Q58063"/>
    <dbReference type="STRING" id="243232.MJ_0647"/>
    <dbReference type="EnsemblBacteria" id="AAB98645">
        <property type="protein sequence ID" value="AAB98645"/>
        <property type="gene ID" value="MJ_0647"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0647"/>
    <dbReference type="eggNOG" id="arCOG09669">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_202275_0_0_2"/>
    <dbReference type="OMA" id="IEMSGIW"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106971" description="Uncharacterized protein MJ0647">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="71" mass="8606" checksum="273B54B8197DC95D" modified="1998-12-15" version="3">MQKLNKHLKKKKQKRKKMKKKLNNEFQEVIDFLKSLPEGRRVYIEMSGIWIEVTKEEAINYLKSKINEKEA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58077</accession>
    <name>Y663_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0663</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0663</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TPP enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64382">
        <property type="entry name" value="G64382"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58077"/>
    <dbReference type="SMR" id="Q58077"/>
    <dbReference type="STRING" id="243232.MJ_0663"/>
    <dbReference type="EnsemblBacteria" id="AAB98655">
        <property type="protein sequence ID" value="AAB98655"/>
        <property type="gene ID" value="MJ_0663"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0663"/>
    <dbReference type="eggNOG" id="arCOG02000">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013748_3_1_2"/>
    <dbReference type="InParanoid" id="Q58077"/>
    <dbReference type="OMA" id="IGVKFGC"/>
    <dbReference type="PhylomeDB" id="Q58077"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14614"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:valine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="PTHR18968"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="chain" id="PRO_0000090834" description="Uncharacterized protein MJ0663">
        <location>
            <begin position="1"/>
            <end position="494"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="494" mass="55355" checksum="1C62956C33D09D82" modified="1996-11-01" version="1">MGGNIKFLEAMVDFLERNVKTIFSYPGEQILPLYNEIEGSSIKNIMVRDERGAGFMADGYARITNYIGVCLATAGPGATNLTTPIATAYKDNSSVLAITGRCQRKYIGKNYFQEVNMDFLNFYKGYFVDKAEVSYIAKAFADCLFNKKPVQLNIPVDLYKEEAKDINITTYTDIYKDDETPSNNIKEIDVKKPLFLIGQGIFGTLSYKEIVKISKILEKINCPIATTFPARGVINEKLENCIGLVGRRGDLKSLLEADKIINIGSSLSYNTYVESVREKLLSKTENIQLKPKSIKELKEFFENLDVKNSSWIYKNSNKFQPSGDYSNKIYEIIKNIPEDAIIVTDAGKHTVFTCLLKTCVIPRNIISSHSFGTMGFGLPASIGVKFGTIDFNIDREVVLISGDGGFLMNVEELQVVAENNLKILMVVMKNNSLAEFCKIKNPNFNKIADAFEIDNCYIENVDEIGSEIKGYLKKNKPLLVVVETENEPLPKPNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q58085</accession>
    <name>RIB7_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <shortName>DAROPP reductase</shortName>
            <shortName>DARP reductase</shortName>
            <ecNumber>1.1.1.302</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase</fullName>
            <shortName>DARIPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>MjaRED</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">arfC</name>
        <name type="ordered locus">MJ0671</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="J. Bacteriol." volume="184" first="1952" last="1957">
            <title>The pyrimidine nucleotide reductase step in riboflavin and F(420) biosynthesis in archaea proceeds by the eukaryotic route to riboflavin.</title>
            <authorList>
                <person name="Graupner M."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="11889103"/>
            <dbReference type="DOI" id="10.1128/JB.184.7.1952-1957.2002"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="FEBS J." volume="275" first="4403" last="4414">
            <title>2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthases of fungi and archaea.</title>
            <authorList>
                <person name="Romisch-Margl W."/>
                <person name="Eisenreich W."/>
                <person name="Haase I."/>
                <person name="Bacher A."/>
                <person name="Fischer M."/>
            </authorList>
            <dbReference type="PubMed" id="18671734"/>
            <dbReference type="DOI" id="10.1111/j.1742-4658.2008.06586.x"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>REACTION STEREOSPECIFICITY</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2006" name="J. Mol. Biol." volume="359" first="1334" last="1351">
            <title>Biosynthesis of riboflavin: structure and properties of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase of Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Chatwell L."/>
                <person name="Krojer T."/>
                <person name="Fidler A."/>
                <person name="Romisch W."/>
                <person name="Eisenreich W."/>
                <person name="Bacher A."/>
                <person name="Huber R."/>
                <person name="Fischer M."/>
            </authorList>
            <dbReference type="PubMed" id="16730025"/>
            <dbReference type="DOI" id="10.1016/j.jmb.2006.04.045"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 6-224 IN COMPLEX WITH NADP</scope>
        <scope>PROTEIN SEQUENCE OF 2-11</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>SUBUNIT</scope>
        <scope>REACTION MECHANISM</scope>
    </reference>
    <comment type="function">
        <text evidence="1 3">Catalyzes an early step in riboflavin biosynthesis, the NAD(P)H-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate. The beta anomer is the authentic substrate, and the alpha anomer can serve as substrate subsequent to spontaneous anomerization. NADPH and NADH function equally well as the reductants. Does not catalyze the reduction of 5-amino-6-(5-phospho-D-ribosylamino)uracil to 5-amino-6-(5-phospho-D-ribitylamino)uracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H(+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2 3">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + H(+) + NADH</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="mass spectrometry" mass="24906.0" method="Electrospray" evidence="2"/>
    <comment type="miscellaneous">
        <text>The protein sequence in PubMed:16730025 comes from protein expressed and processed in E.coli.</text>
    </comment>
    <comment type="miscellaneous">
        <text>Using chirally deuterated NADPH, the enzyme was shown to be A-type reductase catalyzing the transfer of deuterium from the 4(R) position of NADPH to the 1' (S) position of the substrate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HTP reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64383">
        <property type="entry name" value="G64383"/>
    </dbReference>
    <dbReference type="PDB" id="2AZN">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B/C/D/E/F=6-224"/>
    </dbReference>
    <dbReference type="PDBsum" id="2AZN"/>
    <dbReference type="AlphaFoldDB" id="Q58085"/>
    <dbReference type="SMR" id="Q58085"/>
    <dbReference type="STRING" id="243232.MJ_0671"/>
    <dbReference type="EnsemblBacteria" id="AAB98665">
        <property type="protein sequence ID" value="AAB98665"/>
        <property type="gene ID" value="MJ_0671"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0671"/>
    <dbReference type="eggNOG" id="arCOG01484">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036590_4_1_2"/>
    <dbReference type="InParanoid" id="Q58085"/>
    <dbReference type="OMA" id="CECGQEV"/>
    <dbReference type="PhylomeDB" id="Q58085"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14596"/>
    <dbReference type="BRENDA" id="1.1.1.302">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="EvolutionaryTrace" id="Q58085"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="SSF53597"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="initiator methionine" description="Removed" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000135944" description="2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase">
        <location>
            <begin position="2"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="83"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="156"/>
            <end position="159"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="10"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="22"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="48"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="55"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="77"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="100"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="125"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="131"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="150"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="157"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="171"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="188"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="198"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="204"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="215"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="11889103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="16730025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18671734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="16730025"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2AZN"/>
        </source>
    </evidence>
    <sequence length="224" mass="25037" checksum="4D8C15CE291E89D8" modified="1996-11-01" version="1">MVMVMEKKPYIISNVGMTLDGKLATINNDSRISCEEDLIRVHKIRANVDGIMVGIGTVLKDDPRLTVHKIKSDRNPVRIVVDSKLRVPLNARVLNKDAKTIIATTEDTNEEKEKKIKILEDMGVEVVKCGRGKVDLKKLMDILYDKGIKSILLEGGGTLNWGMFKEGLVDEVSVYIAPKIFGGKEAPTYVDGEGFKTVDECVKLELKNFYRLGEGIVLEFKVKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58092</accession>
    <name>TKTC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative transketolase C-terminal section</fullName>
            <shortName>TK</shortName>
            <ecNumber>2.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0679</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>D-glyceraldehyde 3-phosphate + D-sedoheptulose 7-phosphate = aldehydo-D-ribose 5-phosphate + D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:10508"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
        <text evidence="1">Binds 1 thiamine pyrophosphate per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transketolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Could be the product of a pseudogene. Corresponds to the C-terminal of members of this family.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64384">
        <property type="entry name" value="G64384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870184.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58092"/>
    <dbReference type="SMR" id="Q58092"/>
    <dbReference type="STRING" id="243232.MJ_0679"/>
    <dbReference type="EnsemblBacteria" id="AAB98674">
        <property type="protein sequence ID" value="AAB98674"/>
        <property type="gene ID" value="MJ_0679"/>
    </dbReference>
    <dbReference type="GeneID" id="1451545"/>
    <dbReference type="KEGG" id="mja:MJ_0679"/>
    <dbReference type="eggNOG" id="arCOG01051">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009227_1_1_2"/>
    <dbReference type="InParanoid" id="Q58092"/>
    <dbReference type="OMA" id="VINTCDY"/>
    <dbReference type="OrthoDB" id="56448at2157"/>
    <dbReference type="PhylomeDB" id="Q58092"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004802">
        <property type="term" value="F:transketolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="uncertain"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000191915" description="Putative transketolase C-terminal section">
        <location>
            <begin position="1"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="316" mass="34580" checksum="ABAB04A6DBB35199" modified="1996-11-01" version="1">MVKLSGVYKGMRKGYGETLIELGKKYENLVVLDADLSGSTQTAMFAKEFPERFFNAGVAEQNMIGMAAGLATTGKIVFASSFSMFASGRAWEIIRNLVAYPKLNVKIVATHAGITVGEDGASHQMCEDIAIMRAIPNMVVIAPTDYYHTKNVIRTIAEYKGPVYVRMPRRDTEIIYENEEEATFEIGKGKILVDGEDLTIIATGEEVPEALRAGEILKENGISAEIVEMATIKPIDEEIIKKSKDFVVTVEDHSIIGGLGGAVAEVIASNGLNKKLLRIGINDVFGRSGKADELLKYYGLDGESIAKRIMEEMKKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58110</accession>
    <name>Y699_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0699</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0699</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64387">
        <property type="entry name" value="C64387"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58110"/>
    <dbReference type="SMR" id="Q58110"/>
    <dbReference type="STRING" id="243232.MJ_0699"/>
    <dbReference type="EnsemblBacteria" id="AAB98692">
        <property type="protein sequence ID" value="AAB98692"/>
        <property type="gene ID" value="MJ_0699"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0699"/>
    <dbReference type="eggNOG" id="arCOG00692">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012358_1_0_2"/>
    <dbReference type="InParanoid" id="Q58110"/>
    <dbReference type="OMA" id="TDNFMAN"/>
    <dbReference type="PhylomeDB" id="Q58110"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106994" description="Uncharacterized protein MJ0699">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="380" mass="43053" checksum="6AC180A4A0EA7E99" modified="1996-11-01" version="1">MVVFLLLKSQFLYGEDFKLRKGTLIIEEGIIKGFTDEHNEREVIEFKGLVIPSLINAHTHIADNSIKDIGINKTLDELVKPPNGLKHRYLTECSDDLLAEGMKLGLGDMREHGIKYFCDFRENGVRGISLLNKALKCYDYPKAIILGRPIKVDKDEIEEVLKVSNGLGLSGANEFKDDELKLIFKIFKKFKEKDDKKLFAIHAAEHRGAVEYSLNKYGMTEVERLIDLKIKPDFIVHGTHLTDNDLELLKENNIPVVACVRANLSFNVGMPKLNELNDNLLVGIGTDNFMANSPSIFKEMDFIYKLYHIEPKDILRMATINNAKILKLENVGLVDEGFKAVFTFIKPTNAILFSKNIIASVVTRCEKGDVVDFSLMENEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58113</accession>
    <name>DP2S_METJA</name>
    <protein>
        <recommendedName>
            <fullName>DNA polymerase II small subunit</fullName>
            <shortName>Pol II</shortName>
            <ecNumber>2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Exodeoxyribonuclease small subunit</fullName>
            <ecNumber>3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">polB</name>
        <name type="ordered locus">MJ0702</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7"/>
    <dbReference type="EC" id="3.1.11.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64387">
        <property type="entry name" value="F64387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870207.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58113"/>
    <dbReference type="SMR" id="Q58113"/>
    <dbReference type="STRING" id="243232.MJ_0702"/>
    <dbReference type="DNASU" id="1451569"/>
    <dbReference type="EnsemblBacteria" id="AAB98694">
        <property type="protein sequence ID" value="AAB98694"/>
        <property type="gene ID" value="MJ_0702"/>
    </dbReference>
    <dbReference type="GeneID" id="1451569"/>
    <dbReference type="KEGG" id="mja:MJ_0702"/>
    <dbReference type="eggNOG" id="arCOG04455">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027850_1_0_2"/>
    <dbReference type="InParanoid" id="Q58113"/>
    <dbReference type="OMA" id="HVQAALI"/>
    <dbReference type="OrthoDB" id="24499at2157"/>
    <dbReference type="PhylomeDB" id="Q58113"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042575">
        <property type="term" value="C:DNA polymerase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008852">
        <property type="term" value="F:exodeoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000738">
        <property type="term" value="P:DNA catabolic process, exonucleolytic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="PTHR10416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000803">
        <property type="entry name" value="Arc_Pol2_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="SSF56300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000096177" description="DNA polymerase II small subunit">
        <location>
            <begin position="1"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="594" mass="69145" checksum="FA8F24702F2DE8FD" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58115</accession>
    <name>Y704_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0704</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0704</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64387">
        <property type="entry name" value="H64387"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870210.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58115"/>
    <dbReference type="SMR" id="Q58115"/>
    <dbReference type="STRING" id="243232.MJ_0704"/>
    <dbReference type="EnsemblBacteria" id="AAB98705">
        <property type="protein sequence ID" value="AAB98705"/>
        <property type="gene ID" value="MJ_0704"/>
    </dbReference>
    <dbReference type="GeneID" id="1451572"/>
    <dbReference type="KEGG" id="mja:MJ_0704"/>
    <dbReference type="eggNOG" id="arCOG05096">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043915_0_0_2"/>
    <dbReference type="OMA" id="YAVIKCL"/>
    <dbReference type="OrthoDB" id="133364at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106997" description="Uncharacterized protein MJ0704">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="377" mass="44630" checksum="2746A0A96425C285" modified="1996-11-01" version="1">MFAFEGFKWEIVEIFDWKGLKKLQTTFQNYLLILSMIVSIAVVVAIKNYDSLWSLITNSSDSSNFLTVGSILATISALLVSISWVIIQTSSDRLSNILMWIWVRDVRFLTFVVISFTTVFLFILISLTHVQLHVIDYGIIYYVIMLNFLMYALYIKAFANVINPKYAVDLILRDKDIGDKSGGYGDLTDAESRLFAVYEIIEKRIKVGDVYAVIKCLNMINKNFNKYWMFIKDEKREKYLRDFLRILSKLRVEYRKNCLNRKNKPYSKKGLEAFKKTEFIVSFYKTIEEKIKTRDINSINKFLSETYNNISNYEMFNDKTYLEIFVHHLKELLEKYEKEKNENKLDEEIFNELKDELEKLINKCNNKLRELESQNNN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58119</accession>
    <name>Y709_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transporter MJ0709</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0709</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64388">
        <property type="entry name" value="E64388"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58119"/>
    <dbReference type="SMR" id="Q58119"/>
    <dbReference type="STRING" id="243232.MJ_0709"/>
    <dbReference type="TCDB" id="2.A.66.1.26">
        <property type="family name" value="the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98703">
        <property type="protein sequence ID" value="AAB98703"/>
        <property type="gene ID" value="MJ_0709"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0709"/>
    <dbReference type="eggNOG" id="arCOG01731">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012893_0_1_2"/>
    <dbReference type="InParanoid" id="Q58119"/>
    <dbReference type="OMA" id="EFWILLK"/>
    <dbReference type="PhylomeDB" id="Q58119"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000164254" description="Uncharacterized transporter MJ0709">
        <location>
            <begin position="1"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="22"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="163"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="363"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="400"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="450" mass="48495" checksum="52EFE11132C4B79A" modified="1996-11-01" version="1">MKNVEILLDDPKKAVIEVSKPIIVATFIESIYSLVDSIWVSGLGADALAAVGASFPILISIYAVSWGLSIGISSGIARRVGAKNKEEADKVANHAIILALIAGILYIIAVYPNLDTLFSLMGTYGDCKSLAIKYSSILVLGTVIFTICDALYGIFRGEGNTKIVMIASVIGTLTNIILDPIFIYMLNLGISGASYATLIAIIISLLILAYELFIKKSCYVTVKLSKFKPDLKIIADLIRVGIPSALIEITVAVSFFIMTSIIMMVGDSRGLAVYTGALRITEFGFIPMLGLASGATSVIGATYGARSFEKLKTAYFYTIKIGVLMEIIIVALIMLLSPILAYLFTYTKTSMGIHEELVKALRIVPLYLLFTPFILTTSAMFQGIGKGEKSLIISIFRSLICHISYAYLFAVILGLGMFGIYSGLVVGEFTAGIFAFLFGVLAIKALIKSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>Q58164</accession>
    <name>Y754_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0754</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0754</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64394">
        <property type="entry name" value="B64394"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870259.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3Q4N">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.88 A"/>
        <property type="chains" value="A/B=1-185"/>
    </dbReference>
    <dbReference type="PDB" id="3Q4O">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.34 A"/>
        <property type="chains" value="A=11-185"/>
    </dbReference>
    <dbReference type="PDB" id="3Q4Q">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="A=11-185"/>
    </dbReference>
    <dbReference type="PDB" id="3Q4R">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.60 A"/>
        <property type="chains" value="A=11-185"/>
    </dbReference>
    <dbReference type="PDBsum" id="3Q4N"/>
    <dbReference type="PDBsum" id="3Q4O"/>
    <dbReference type="PDBsum" id="3Q4Q"/>
    <dbReference type="PDBsum" id="3Q4R"/>
    <dbReference type="AlphaFoldDB" id="Q58164"/>
    <dbReference type="SMR" id="Q58164"/>
    <dbReference type="STRING" id="243232.MJ_0754"/>
    <dbReference type="DNASU" id="1451631"/>
    <dbReference type="EnsemblBacteria" id="AAB98756">
        <property type="protein sequence ID" value="AAB98756"/>
        <property type="gene ID" value="MJ_0754"/>
    </dbReference>
    <dbReference type="GeneID" id="1451631"/>
    <dbReference type="KEGG" id="mja:MJ_0754"/>
    <dbReference type="eggNOG" id="arCOG03957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051317_1_2_2"/>
    <dbReference type="InParanoid" id="Q58164"/>
    <dbReference type="OMA" id="YITSAYF"/>
    <dbReference type="OrthoDB" id="80859at2157"/>
    <dbReference type="PhylomeDB" id="Q58164"/>
    <dbReference type="EvolutionaryTrace" id="Q58164"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd01048">
        <property type="entry name" value="Ferritin_like_AB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019243">
        <property type="entry name" value="DUF2202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09968">
        <property type="entry name" value="DUF2202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="SSF47240"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107015" description="Uncharacterized protein MJ0754">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="130"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="168"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="3Q4O"/>
        </source>
    </evidence>
    <sequence length="185" mass="21766" checksum="2CE5EC9D424895F6" modified="1996-11-01" version="1">MLEYISSLPKQPISEEEKEGLIEMREEEKLARDVYLTLYNKWKLQIFKNIAESEQTHMDAVKYLLEKYNIPDPVKNDSIGVFSNPKFEELYKKLVEKGDKSEVDALKVGATIEDLDIADLEKWINKTDNEDIKFVYENLMKGSRNHMRAFVRMLNNYGSNYTPQYISKEEYEEIISSSTERGMNR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58185</accession>
    <name>MPTA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>GTP cyclohydrolase MptA</fullName>
            <ecNumber>3.5.4.39</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>GTP cyclohydrolase IV</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">mptA</name>
        <name type="ordered locus">MJ0775</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="Biochemistry" volume="46" first="6658" last="6667">
            <title>Characterization of an Fe(2+)-dependent archaeal-specific GTP cyclohydrolase, MptA, from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Grochowski L.L."/>
                <person name="Xu H."/>
                <person name="Leung K."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="17497938"/>
            <dbReference type="DOI" id="10.1021/bi700052a"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>MUTAGENESIS OF GLU-47; HIS-98; HIS-197; HIS-290 AND HIS-292</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Converts GTP to 7,8-dihydro-D-neopterin 2',3'-cyclic phosphate, the first intermediate in the biosynthesis of coenzyme methanopterin. It is also able to utilize a variety of GTP analogs as substrates, including GDP, beta,gamma-methylene-GTP and GTP-[gamma-thio].</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>GTP + H2O = 7,8-dihydroneopterin 2',3'-cyclic phosphate + diphosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25860"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58854"/>
            <dbReference type="EC" id="3.5.4.39"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe(2+) ion per subunit. Mn(2+) and Zn(2+) can be used to a lesser extent, but not at a relevant physiological concentration.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">Inhibited by GTP concentrations greater than 0.3 mM and by 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone 5'-phosphate (fapyGMP). Partial inhibition is observed when 2 mM GMP, dGTP, or 7-methyl-GTP was included along with 2 mM GTP.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <Vmax evidence="1">13.0 nmol/min/mg enzyme with GDP as substrate (at pH 7.2)</Vmax>
            <Vmax evidence="1">30.0 nmol/min/mg enzyme with GTP as substrate (at pH 7.2)</Vmax>
            <Vmax evidence="1">39.0 nmol/min/mg enzyme with GTP-[gamma-thio] as substrate (at pH 7.2)</Vmax>
            <Vmax evidence="1">3.0 nmol/min/mg enzyme with beta,gamma-methylene-GTP as substrate (at pH 7.2)</Vmax>
            <text>Other purine nucleotides including ATP, ITP, dGTP, GMP, and 7-methyl-GTP do not serve as substrates.</text>
        </kinetics>
        <phDependence>
            <text evidence="1">Optimum pH is 6.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="1">MptA loses 27% activity when it incubates for 10 min at 80 degrees Celsius, 60% activity at 90 degrees Celsius, and 89% activity at 100 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text>MptA is the archetype of a new class of GTP cyclohydrolases that catalyzes a series of reactions most similar to that seen with GTPCHI but unique in that the cyclic phosphate is the product.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP cyclohydrolase IV family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98765" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.5.4.39"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98765.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64396">
        <property type="entry name" value="G64396"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496622.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58185"/>
    <dbReference type="SMR" id="Q58185"/>
    <dbReference type="STRING" id="243232.MJ_0775"/>
    <dbReference type="DNASU" id="1451652"/>
    <dbReference type="EnsemblBacteria" id="AAB98765">
        <property type="protein sequence ID" value="AAB98765"/>
        <property type="gene ID" value="MJ_0775"/>
    </dbReference>
    <dbReference type="GeneID" id="1451652"/>
    <dbReference type="KEGG" id="mja:MJ_0775"/>
    <dbReference type="eggNOG" id="arCOG04301">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062816_1_0_2"/>
    <dbReference type="InParanoid" id="Q58185"/>
    <dbReference type="OMA" id="PCSQGMS"/>
    <dbReference type="OrthoDB" id="109805at2157"/>
    <dbReference type="PhylomeDB" id="Q58185"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14599"/>
    <dbReference type="BRENDA" id="3.5.4.39">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044682">
        <property type="term" value="F:archaeal-specific GTP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003933">
        <property type="term" value="F:GTP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001116">
        <property type="term" value="P:methanopterin-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01527_A">
        <property type="entry name" value="GTP_cyclohydrol_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003801">
        <property type="entry name" value="GTP_cyclohydrolase_FolE2/MptA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022840">
        <property type="entry name" value="GTP_cyclohydrolase_MptA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36445">
        <property type="entry name" value="PTHR36445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02649">
        <property type="entry name" value="GCHY-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00294">
        <property type="entry name" value="TIGR00294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000147743" description="GTP cyclohydrolase MptA">
        <location>
            <begin position="1"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="site" description="May be catalytically important">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Unchanged." evidence="1">
        <original>E</original>
        <variation>D</variation>
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Unchanged." evidence="1">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="98"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in specific activity." evidence="1">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="197"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in specific activity." evidence="1">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="290"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Strong decrease in specific activity." evidence="1">
        <original>H</original>
        <variation>N</variation>
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="17497938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="313" mass="35873" checksum="4CCA67352C1773CF" modified="2009-05-05" version="2">MNWRCDVQNFEPDVKISLTRVGVTNLKKLVRLKRTNKRPIILLSTFEVFVNLPSSQKGIHMSRNPEVIEGIIDEALELESYEMETICEEIVKRLFEKHEYATEAEVFMVSDFMTKEKSPISGKYSQEIHKIMGGAKGIKKDDEIELTKIVGAEVVGITACPCAQNLIKEICIKNLKEKGFSDEDIDKILDSVIFATHNQRGIGRIILEVPTGYDIEIMDIIEIIKKSMSAEIHGILKRADEAYVVEQSHKNPKFVEDCVREMAKRVVEKFKHLPDETKVLIRQINMESIHRHDAFAEKVATLGELRRELLSYE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58203</accession>
    <name>Y793_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0793</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0793</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ0706 and Synechocystis PCC 6803 slr1478.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64399">
        <property type="entry name" value="A64399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870301.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58203"/>
    <dbReference type="STRING" id="243232.MJ_0793"/>
    <dbReference type="DNASU" id="1451673"/>
    <dbReference type="EnsemblBacteria" id="AAB98796">
        <property type="protein sequence ID" value="AAB98796"/>
        <property type="gene ID" value="MJ_0793"/>
    </dbReference>
    <dbReference type="GeneID" id="1451673"/>
    <dbReference type="KEGG" id="mja:MJ_0793"/>
    <dbReference type="eggNOG" id="arCOG01996">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099320_1_0_2"/>
    <dbReference type="InParanoid" id="Q58203"/>
    <dbReference type="OMA" id="KNNMKVY"/>
    <dbReference type="OrthoDB" id="120839at2157"/>
    <dbReference type="PhylomeDB" id="Q58203"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107044" description="Uncharacterized protein MJ0793">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="178" mass="19804" checksum="95B9049D585D12AA" modified="1996-11-01" version="1">MKIPIILTLMLFSLGFIFGFISINNLSKINDKDLSNYIPNIQFNFPSILTNNLKVIFLMLAGSITFGLSTFINLIFNGFNVGVLIGSISLTNEPLKLITALILPHGIFEISAMLISAVAGFKIPYKVTLYLLDKKEKPLTEEDIKDFLKLSLISIILIVIAAFIEVYITPKIATYLLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58226</accession>
    <name>Y816_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0816</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0816</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64401">
        <property type="entry name" value="H64401"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870327.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58226"/>
    <dbReference type="STRING" id="243232.MJ_0816"/>
    <dbReference type="EnsemblBacteria" id="AAB98818">
        <property type="protein sequence ID" value="AAB98818"/>
        <property type="gene ID" value="MJ_0816"/>
    </dbReference>
    <dbReference type="GeneID" id="1451699"/>
    <dbReference type="KEGG" id="mja:MJ_0816"/>
    <dbReference type="eggNOG" id="arCOG04823">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077587_0_0_2"/>
    <dbReference type="OMA" id="PGGWVIG"/>
    <dbReference type="OrthoDB" id="66950at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107061" description="Uncharacterized protein MJ0816">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <sequence length="297" mass="34913" checksum="724C69341CB90230" modified="1996-11-01" version="1">MQDKQLLQLKEQIINDIKKRVLKIREDLGLKKVEFDIVDIEIEGKVLIIYTKNRTDKSTIIGPGGWVVGKLREEMKNRFEIIRVEDYTDKVLFEEIVKAIKSLFDDEVIQDICNYFLYRKIPKEKKNIICLIHCQYDLYALDILSNIFNVKAITYDFPALIPNKTKKKIANFLNNKDIGHKFLKLDITKDKIKNLIDSFPYGFLKDKIIEDLEGYVFTSCLDTAVFKYNKGTIINFFELFPIKIKKDENYLNYCPLCIQSCKIDKNKEKFIKKVVKEVYKGFKEPTDASEEILSMIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="128" xmlns="http://uniprot.org/uniprot">
    <accession>Q58242</accession>
    <name>Y832_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0832</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Mja rnr-1 intein</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName>Mja rnr-2 intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ordered locus">MJ0832</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="4">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64403">
        <property type="entry name" value="H64403"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58242"/>
    <dbReference type="SMR" id="Q58242"/>
    <dbReference type="STRING" id="243232.MJ_0832"/>
    <dbReference type="EnsemblBacteria" id="AAB98834">
        <property type="protein sequence ID" value="AAB98834"/>
        <property type="gene ID" value="MJ_0832"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0832"/>
    <dbReference type="eggNOG" id="arCOG03145">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03154">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG03714">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG04889">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002707_0_0_2"/>
    <dbReference type="InParanoid" id="Q58242"/>
    <dbReference type="OMA" id="CQFLGLF"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031250">
        <property type="term" value="C:anaerobic ribonucleoside-triphosphate reductase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009265">
        <property type="term" value="P:2'-deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
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        <property type="match status" value="2"/>
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        <property type="entry name" value="LAGLIDADG_3"/>
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        <property type="match status" value="3"/>
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    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="intein_Cterm"/>
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    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000013999" description="Uncharacterized protein MJ0832, 1st part" evidence="1">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014000" description="Mja rnr-1 intein" evidence="1">
        <location>
            <begin position="338"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014001" description="Uncharacterized protein MJ0832, 2nd part" evidence="1">
        <location>
            <begin position="791"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014002" description="Mja rnr-2 intein" evidence="1">
        <location>
            <begin position="1059"/>
            <end position="1591"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014003" description="Uncharacterized protein MJ0832, 3rd part" evidence="1">
        <location>
            <begin position="1592"/>
            <end position="1750"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-cone" evidence="3">
        <location>
            <begin position="14"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease 1" evidence="2">
        <location>
            <begin position="507"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease 2" evidence="2">
        <location>
            <begin position="1286"/>
            <end position="1426"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="1750" mass="203281" checksum="6D6893B770A25359" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58311</accession>
    <name>FLAJ_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Flagella accessory protein J</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">flaJ</name>
        <name type="ordered locus">MJ0901</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.voltae FlaJ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">To M.jannaschii MJ1286.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="E64412"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58311"/>
    <dbReference type="STRING" id="243232.MJ_0901"/>
    <dbReference type="PRIDE" id="Q58311"/>
    <dbReference type="EnsemblBacteria" id="AAB98904">
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        <property type="gene ID" value="MJ_0901"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0901"/>
    <dbReference type="eggNOG" id="arCOG01809">
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    <dbReference type="HOGENOM" id="CLU_479528_0_0_2"/>
    <dbReference type="InParanoid" id="Q58311"/>
    <dbReference type="OMA" id="DRLWHTG"/>
    <dbReference type="PhylomeDB" id="Q58311"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005886">
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        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0974">Archaeal flagellum</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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    <keyword id="KW-1133">Transmembrane helix</keyword>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="73"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="225"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="302"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="446"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="562" mass="63601" checksum="F87DC2BDB763A351" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="82" xmlns="http://uniprot.org/uniprot">
    <accession>Q58313</accession>
    <name>Y903_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0903</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0903</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
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            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64412">
        <property type="entry name" value="G64412"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58313"/>
    <dbReference type="STRING" id="243232.MJ_0903"/>
    <dbReference type="EnsemblBacteria" id="AAB98908">
        <property type="protein sequence ID" value="AAB98908"/>
        <property type="gene ID" value="MJ_0903"/>
    </dbReference>
    <dbReference type="GeneID" id="1451792"/>
    <dbReference type="KEGG" id="mja:MJ_0903"/>
    <dbReference type="eggNOG" id="arCOG05057">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015662_0_0_2"/>
    <dbReference type="OMA" id="YEVKTWI"/>
    <dbReference type="OrthoDB" id="2406at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107095" description="Uncharacterized protein MJ0903">
        <location>
            <begin position="1"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="643" mass="72968" checksum="8FBD01772DBD88DD" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-05-04" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58327</accession>
    <name>NPPNK_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Bifunctional NADP phosphatase/NAD kinase</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="2">NAD kinase</fullName>
                <ecNumber evidence="2">2.7.1.23</ecNumber>
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            <alternativeName>
                <fullName evidence="2">ATP-dependent NAD kinase</fullName>
            </alternativeName>
            <alternativeName>
                <fullName>Poly(P)-dependent NAD kinase</fullName>
                <shortName>PPNK</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName>NADP phosphatase</fullName>
                <shortName>NADPase</shortName>
                <shortName>pNPPase</shortName>
                <ecNumber>3.1.3.-</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name type="ordered locus">MJ0917</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="J. Biol. Chem." volume="280" first="39200" last="39207">
            <title>MJ0917 in archaeon Methanococcus jannaschii is a novel NADP phosphatase/NAD kinase.</title>
            <authorList>
                <person name="Kawai S."/>
                <person name="Fukuda C."/>
                <person name="Mukai T."/>
                <person name="Murata K."/>
            </authorList>
            <dbReference type="PubMed" id="16192277"/>
            <dbReference type="DOI" id="10.1074/jbc.M506426200"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-8</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the regulation of the intracellular balance between NAD(H) and NADP(H), and is a key enzyme in the biosynthesis of NADP. Catalyzes the phosphorylation and dephosphorylation of NAD and NADP, respectively. Although it shows conflicting dual activities and is able to supply NADP, it seems that its physiological role is to prevent excess accumulation of NADP. Kinase can use ATP and other nucleoside triphosphates (UTP, TTP, CTP, GTP) as well as inorganic polyphosphate (poly(P)) as phosphoryl donors, however poly(P) is not considered to be the physiological phosphoryl donor. NAD is the preferred substrate for the kinase, but NADH can also be used as phosphoryl acceptor. Phosphatase can use NADP or NADPH as phosphoryl donor, but NADP is the preferred substrate. Phosphatase also has an activity toward the terminal phosphate group at C-2 of adenosine in 2'-AMP and toward the phosphate group at C-1 of fructose 1,6-bisphosphate, but not toward inositol 1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>ATP + NAD(+) = ADP + H(+) + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>H2O + NADP(+) = NAD(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:28050"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Mg(2+). NAD and pNPPase kinase show maximum activities at 50 and 20 mM magnesium, respectively.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Phosphatase activity is slightly inhibited by ADP, NADPH and ATP, and moderately inhibited by NAD and 5'-AMP. Kinase activity is slightly inhibited by ADP and NADP.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">0.35 mM for ATP (for kinase activity at pH 8.5 and at 85 degrees Celsius)</KM>
            <KM evidence="3">3 mM for NAD (for kinase activity at pH 8.5 and at 85 degrees Celsius)</KM>
            <Vmax evidence="3">93.4 umol/min/mg enzyme toward ATP (for kinase activity at pH 8.5 and at 85 degrees Celsius)</Vmax>
            <Vmax evidence="3">99.2 umol/min/mg enzyme toward NAD (for kinase activity at pH 8.5 and at 85 degrees Celsius)</Vmax>
            <Vmax evidence="3">212.0 umol/min/mg enzyme toward NADP (for phosphatase activity at pH 8.5 and at 85 degrees Celsius)</Vmax>
            <Vmax evidence="3">212.0 umol/min/mg enzyme toward fructose 1,6-bisphosphate (for phosphatase activity at pH 8.5 and at 85 degrees Celsius)</Vmax>
            <Vmax evidence="3">236.0 umol/min/mg enzyme toward NADPH (for phosphatase activity at pH 8.5 and at 85 degrees Celsius)</Vmax>
            <text>kcat is 399 sec(-1) for kinase activity with ATP as substrate. kcat is 424 sec(-1) for kinase activity with NAD as substrate. kcat is 906 sec(-1) for phosphatase activity with fructose 1,6-bisphosphate and NADP as substrates. kcat is 1007 sec(-1) for phosphatase activity with NADPH as substrate.</text>
        </kinetics>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 100 degrees Celsius for phosphatase and kinase activities. Both are inactive below 60 degrees Celsius.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">The phosphatase is inert toward the substrates of NAD kinase (NAD, NADH, ATP, and poly(P)). This demonstrates that the phosphatase activity never interferes with the NAD kinase activity by degrading its substrates (PubMed:16192277).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the NAD kinase family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB98922.1"/>
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        <property type="protein sequence ID" value="AAB98922"/>
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        <property type="entry name" value="ATP-NAD_kinase_N"/>
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        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021175">
        <property type="entry name" value="Bifunctional_PpnK_predicted"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
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    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036641">
        <property type="entry name" value="Bifunctional_PpnK_predicted"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="SSF111331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0511">Multifunctional enzyme</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000120700" description="Bifunctional NADP phosphatase/NAD kinase">
        <location>
            <begin position="1"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="NADP phosphatase">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="NAD kinase">
        <location>
            <begin position="302"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="362"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="362"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="367"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="436"/>
            <end position="437"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="447"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="464"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="474"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="477"/>
            <end position="482"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="16192277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="16192277"/>
        </source>
    </evidence>
    <sequence length="574" mass="64119" checksum="89A1AF944BD99DB1" modified="1998-06-01" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="77" xmlns="http://uniprot.org/uniprot">
    <accession>Q58399</accession>
    <name>Y992_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0992</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0992</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.jannaschii MJ0628.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="H64423"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="protein sequence ID" value="AAB98997"/>
        <property type="gene ID" value="MJ_0992"/>
    </dbReference>
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    <dbReference type="KEGG" id="mja:MJ_0992"/>
    <dbReference type="eggNOG" id="arCOG06576">
        <property type="taxonomic scope" value="Archaea"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107135" description="Uncharacterized protein MJ0992">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="177" mass="20903" checksum="E5FE519F740853C5" modified="1996-11-01" version="1">MFNIDEFKEIAEKLPTFKSLPNEGKYRTAIGRYYYCIFLKLREIVKEIEQDREDGIYELLNSGKAHKALPAYFRTLSDKIRVDNLKNDLITLAEALEDLRKLRNMCDYDVDVSISFTKVIEAEIDIEIIEQTITNLSYQKPKSKTKIVGLKNVLEYFKDKDNLPTYNEVINIMYRRR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58404</accession>
    <name>Y998_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0998</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0998</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64424">
        <property type="entry name" value="E64424"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58404"/>
    <dbReference type="SMR" id="Q58404"/>
    <dbReference type="EnsemblBacteria" id="AAB99006">
        <property type="protein sequence ID" value="AAB99006"/>
        <property type="gene ID" value="MJ_0998"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0998"/>
    <dbReference type="HOGENOM" id="CLU_1149815_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107137" description="Uncharacterized protein MJ0998">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="110"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="241" mass="27835" checksum="CC8495C38B098D02" modified="1996-11-01" version="1">MKSLYALIFLLFVIVVSYIFNGLWSVFNIKHVFWVNLFLLIAFHPQHLDGLIILLLIPLKIFSNFKLKLQCIIALVGILLTIIKGVIKSGFGWILRILFFFVRMMTVSFINLVVFSILLTSVYVLGYVAFLKPDDIQFGTLYTAFGGLALLGAGIKIIQHFIKQSEEIAQEEFKKWYETEVKNFMYSLFITAKNAFPKFLDDLLAKGVVSQEEYQNLIKLYSHILARILKNDEEKMKIPTF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58407</accession>
    <name>COFF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Coenzyme gamma-F420-2:alpha-L-glutamate ligase</fullName>
            <ecNumber>6.3.2.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">cofF</name>
        <name type="ordered locus">MJ1001</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Proc. Natl. Acad. Sci. U.S.A." volume="100" first="9785" last="9790">
            <title>Glutathione synthetase homologs encode alpha-L-glutamate ligases for methanogenic coenzyme F420 and tetrahydrosarcinapterin biosyntheses.</title>
            <authorList>
                <person name="Li H."/>
                <person name="Xu H."/>
                <person name="Graham D.E."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="12909715"/>
            <dbReference type="DOI" id="10.1073/pnas.1733391100"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the ATP-dependent addition of one alpha-linked L-glutamate molecule to coenzyme gamma-F420-2, producing alpha-F420-3, the major form of coenzyme F420 found in M.jannaschii. Thus, caps the gamma-polyglutamate tail of coenzyme F420 with a terminal alpha-linked glutamate. Prefers ATP to other purine nucleotide triphosphates; GTP gives about 25% of the activity observed with ATP. Cannot catalyze the addition of the following amino acids or analogs: D-glutamate, beta-glutamate, L-aspartate, L-glutamine, L-alpha-aminoadipate, or D,L-2-amino-4-phosphono-butyrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>ATP + L-glutamate + oxidized coenzyme F420-2 = ADP + H(+) + oxidized coenzyme alpha-F420-3 + phosphate</text>
            <dbReference type="Rhea" id="RHEA:42332"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57922"/>
            <dbReference type="ChEBI" id="CHEBI:59923"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="3">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Inhibited by KCl.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">1.2 uM for coenzyme F420-2</KM>
            <Vmax evidence="3">0.72 nmol/min/mg enzyme</Vmax>
        </kinetics>
        <temperatureDependence>
            <text evidence="3">Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RimK family. CofF subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="4">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99004" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.2.32"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99004.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64424">
        <property type="entry name" value="H64424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496695.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58407"/>
    <dbReference type="SMR" id="Q58407"/>
    <dbReference type="STRING" id="243232.MJ_1001"/>
    <dbReference type="EnsemblBacteria" id="AAB99004">
        <property type="protein sequence ID" value="AAB99004"/>
        <property type="gene ID" value="MJ_1001"/>
    </dbReference>
    <dbReference type="GeneID" id="1451898"/>
    <dbReference type="KEGG" id="mja:MJ_1001"/>
    <dbReference type="eggNOG" id="arCOG01589">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_975255_0_0_2"/>
    <dbReference type="InParanoid" id="Q58407"/>
    <dbReference type="OMA" id="YEHKVFY"/>
    <dbReference type="OrthoDB" id="42812at2157"/>
    <dbReference type="PhylomeDB" id="Q58407"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-12190"/>
    <dbReference type="BRENDA" id="6.3.2.32">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58407"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="MENGO"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031039">
        <property type="entry name" value="F420_CofF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="RpS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR04443">
        <property type="entry name" value="F420_CofF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000205498" description="Coenzyme gamma-F420-2:alpha-L-glutamate ligase">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="101"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12909715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="288" mass="33112" checksum="08443376FDC5E61D" modified="2005-04-26" version="2">MVKITILSPEGRSCSVWSLKNEIEKLGAKCDIFLLSSPENLMSHDFKLETDLIHSRCGIGDYFDRLTLYSWQFINALEVEGCRFINPIKTLYLTSDKFKCIKLLAKNKIKTPKTALIRDYEDAVKFIEKYNLRFPVVIKNSFSKCGLKVFMARNYDELKQLTKNAIWEGKLIQEFIDFKENDLYRDMRILVVDGEVVGGYRRVSRDFRTNLYLGNVVEKLNIDEELEELALKCADLSEAVILGVDILPTKDNYYVIELNSSPGTKGFRDIGINADKKIAEALVRYAKS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="107" xmlns="http://uniprot.org/uniprot">
    <accession>Q58415</accession>
    <name>PHOU_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphate-specific transport system accessory protein PhoU homolog</fullName>
            <shortName>Pst system accessory protein PhoU homolog</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1009</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PhoU family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64425">
        <property type="entry name" value="H64425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870522.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58415"/>
    <dbReference type="SMR" id="Q58415"/>
    <dbReference type="STRING" id="243232.MJ_1009"/>
    <dbReference type="EnsemblBacteria" id="AAB99011">
        <property type="protein sequence ID" value="AAB99011"/>
        <property type="gene ID" value="MJ_1009"/>
    </dbReference>
    <dbReference type="GeneID" id="1451906"/>
    <dbReference type="KEGG" id="mja:MJ_1009"/>
    <dbReference type="eggNOG" id="arCOG00232">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078518_3_0_2"/>
    <dbReference type="InParanoid" id="Q58415"/>
    <dbReference type="OMA" id="WKHGIET"/>
    <dbReference type="OrthoDB" id="76473at2157"/>
    <dbReference type="PhylomeDB" id="Q58415"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:cellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000186">
        <property type="term" value="P:negative regulation of phosphate transmembrane transport"/>
        <property type="evidence" value="ECO:0000250"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="P_transport_PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PTHR42930"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0592">Phosphate transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000155188" description="Phosphate-specific transport system accessory protein PhoU homolog">
        <location>
            <begin position="1"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="236" mass="27426" checksum="900834E6BBE649D6" modified="1996-11-01" version="1">MPKKFEEILKEVENDLIEMAELCAEQTENAVKAFIESDRELAKQVRKRDTTIDLMEMKIEEKCIKAIALYQPVSGDLRELMTAIKISSKLEKVGDNASKICKILLKSDVEGNRKNELLIVMKDYLINMLKNAMIAFKTRDESLARDVYNMDKRLDDLYEQLYRSMISKIIENPKNLTLYTEIIFAGKYLERSGNIVASIGDRIVYMITGERIKEEEIEEELKKEKDIEKNIDQIND</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q58425</accession>
    <name>CARA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Carbamoyl-phosphate synthase small chain</fullName>
            <ecNumber>6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Carbamoyl-phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">carA</name>
        <name type="ordered locus">MJ1019</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CarA family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870532.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58425"/>
    <dbReference type="SMR" id="Q58425"/>
    <dbReference type="STRING" id="243232.MJ_1019"/>
    <dbReference type="MEROPS" id="C26.A33"/>
    <dbReference type="EnsemblBacteria" id="AAB99021">
        <property type="protein sequence ID" value="AAB99021"/>
        <property type="gene ID" value="MJ_1019"/>
    </dbReference>
    <dbReference type="GeneID" id="1451916"/>
    <dbReference type="KEGG" id="mja:MJ_1019"/>
    <dbReference type="eggNOG" id="arCOG00064">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035901_2_1_2"/>
    <dbReference type="InParanoid" id="Q58425"/>
    <dbReference type="OMA" id="CFNTGMT"/>
    <dbReference type="OrthoDB" id="35557at2157"/>
    <dbReference type="PhylomeDB" id="Q58425"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005951">
        <property type="term" value="C:carbamoyl-phosphate synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="SSF52021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="SSF52317"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000112358" description="Carbamoyl-phosphate synthase small chain">
        <location>
            <begin position="1"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1">
        <location>
            <begin position="171"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="354" mass="39920" checksum="4D8F07776CA65F9F" modified="1998-12-15" version="2">MEAVLILEDGTILKGKGFGAEKEVFGELVFTTVMTGYVEVLTDPSYKGQIVMMTYPLEGNYGVKKDWFESDGIKAEGFVVREVTSKALDDFLKEYDIPGIQDIDTRFLTRKIRDKGVVKSCLKVAEEISDDEISELLERVKRYSDISDIDLVPLVSTKEPKIHKTANPKARCVLIDCGVKLNIIRSLVQRNCEVIQVPYNTKYDEILEYKPDFVLISNGPGDPARLKEVIKNIKNLIGVVPITGICLGNQLLSLAFGGETYKMKFGHRGGNQPVKDLKTQKVYITSQNHGFAVRKESLPDDVEVSFINLNDMTVEGIRHKDLPIFSVQFHPEARPGPHDTMFLFDEMIKLKDRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58434</accession>
    <name>TOP6B_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Type 2 DNA topoisomerase 6 subunit B</fullName>
            <ecNumber evidence="1">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Type II DNA topoisomerase VI subunit B</fullName>
            <shortName evidence="1">TopoVI-B</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">top6B</name>
        <name type="ordered locus">MJ1028</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TOP6B family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99032" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99032.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64428">
        <property type="entry name" value="C64428"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58434"/>
    <dbReference type="SMR" id="Q58434"/>
    <dbReference type="STRING" id="243232.MJ_1028"/>
    <dbReference type="EnsemblBacteria" id="AAB99032">
        <property type="protein sequence ID" value="AAB99032"/>
        <property type="gene ID" value="MJ_1028"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1028"/>
    <dbReference type="eggNOG" id="arCOG01165">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006403_0_0_2"/>
    <dbReference type="InParanoid" id="Q58434"/>
    <dbReference type="OMA" id="TRIELEM"/>
    <dbReference type="PhylomeDB" id="Q58434"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006268">
        <property type="term" value="P:DNA unwinding involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00823">
        <property type="entry name" value="TopoIIB_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00322">
        <property type="entry name" value="Top6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribosomal_S5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005734">
        <property type="entry name" value="TopoVI_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF4">
        <property type="entry name" value="PTHR10871:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006553">
        <property type="entry name" value="TopoVI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="SSF54211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="SSF55874"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01052">
        <property type="entry name" value="top6b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0799">Topoisomerase</keyword>
    <feature type="chain" id="PRO_0000145462" description="Type 2 DNA topoisomerase 6 subunit B">
        <location>
            <begin position="1"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="101"/>
            <end position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="111"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="660" mass="75674" checksum="8F6E4606EE8833C3" modified="2001-02-21" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="126" xmlns="http://uniprot.org/uniprot">
    <accession>Q58443</accession>
    <name>RPO5_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo5</name>
        <name evidence="1" type="synonym">rpoH</name>
        <name type="ordered locus">MJ1039</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Mol. Biol." volume="287" first="753" last="760">
            <title>RNA polymerase subunit H features a beta-ribbon motif within a novel fold that is present in archaea and eukaryotes.</title>
            <authorList>
                <person name="Thiru A."/>
                <person name="Hodach M."/>
                <person name="Eloranta J.J."/>
                <person name="Kostourou V."/>
                <person name="Weinzierl R.O."/>
                <person name="Matthews S."/>
            </authorList>
            <dbReference type="PubMed" id="10191143"/>
            <dbReference type="DOI" id="10.1006/jmbi.1999.2638"/>
        </citation>
        <scope>STRUCTURE BY NMR</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64429">
        <property type="entry name" value="F64429"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870552.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1HMJ">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-78"/>
    </dbReference>
    <dbReference type="PDBsum" id="1HMJ"/>
    <dbReference type="AlphaFoldDB" id="Q58443"/>
    <dbReference type="BMRB" id="Q58443"/>
    <dbReference type="SMR" id="Q58443"/>
    <dbReference type="STRING" id="243232.MJ_1039"/>
    <dbReference type="EnsemblBacteria" id="AAB99042">
        <property type="protein sequence ID" value="AAB99042"/>
        <property type="gene ID" value="MJ_1039"/>
    </dbReference>
    <dbReference type="GeneID" id="1451936"/>
    <dbReference type="KEGG" id="mja:MJ_1039"/>
    <dbReference type="eggNOG" id="arCOG04258">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058320_4_0_2"/>
    <dbReference type="InParanoid" id="Q58443"/>
    <dbReference type="OMA" id="PKIYHDD"/>
    <dbReference type="OrthoDB" id="123463at2157"/>
    <dbReference type="PhylomeDB" id="Q58443"/>
    <dbReference type="EvolutionaryTrace" id="Q58443"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed 5'-3' RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="PTHR10535"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="SSF55287"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000146092" description="DNA-directed RNA polymerase subunit Rpo5">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <sequence length="78" mass="9001" checksum="CDF2DAF342E6D41B" modified="1997-11-01" version="1">MKVTDHILVPKHEIVPKEEVEEILKRYNIKIQQLPKIYEDDPVIQEIGAKEGDVVRVIRKSPTAGVSIAYRLVIKRII</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>Q58454</accession>
    <name>Y1054_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1054</fullName>
            <ecNumber>1.1.1.-</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Mja UDPGD intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ordered locus">MJ1054</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="4">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64431">
        <property type="entry name" value="E64431"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870567.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58454"/>
    <dbReference type="SMR" id="Q58454"/>
    <dbReference type="STRING" id="243232.MJ_1054"/>
    <dbReference type="EnsemblBacteria" id="AAB99056">
        <property type="protein sequence ID" value="AAB99056"/>
        <property type="gene ID" value="MJ_1054"/>
    </dbReference>
    <dbReference type="GeneID" id="1451951"/>
    <dbReference type="KEGG" id="mja:MJ_1054"/>
    <dbReference type="eggNOG" id="arCOG00253">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_320207_0_0_2"/>
    <dbReference type="InParanoid" id="Q58454"/>
    <dbReference type="OMA" id="IKWEFLK"/>
    <dbReference type="OrthoDB" id="47911at2157"/>
    <dbReference type="PhylomeDB" id="Q58454"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="SSF48179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="SSF52413"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="SSF55608"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000035997" description="Uncharacterized protein MJ1054, 1st part" evidence="2">
        <location>
            <begin position="1"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000035998" description="Mja UDPGD intein" evidence="2">
        <location>
            <begin position="261"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000035999" description="Uncharacterized protein MJ1054, 2nd part" evidence="2">
        <location>
            <begin position="715"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="3">
        <location>
            <begin position="468"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="2"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="895" mass="102615" checksum="B511AA68CCA41445" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58476</accession>
    <name>Y1076_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ1076</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1076</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1997" name="Science" volume="275" first="1489" last="1490">
            <title>Evidence for a family of archaeal ATPases.</title>
            <authorList>
                <person name="Koonin E.V."/>
            </authorList>
            <dbReference type="PubMed" id="9045616"/>
            <dbReference type="DOI" id="10.1126/science.275.5305.1489"/>
        </citation>
        <scope>SIMILARITY</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64434">
        <property type="entry name" value="C64434"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870588.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58476"/>
    <dbReference type="STRING" id="243232.MJ_1076"/>
    <dbReference type="EnsemblBacteria" id="AAB99081">
        <property type="protein sequence ID" value="AAB99081"/>
        <property type="gene ID" value="MJ_1076"/>
    </dbReference>
    <dbReference type="GeneID" id="1451972"/>
    <dbReference type="KEGG" id="mja:MJ_1076"/>
    <dbReference type="eggNOG" id="arCOG03407">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068608_0_0_2"/>
    <dbReference type="InParanoid" id="Q58476"/>
    <dbReference type="OMA" id="IRRWINI"/>
    <dbReference type="OrthoDB" id="132662at2157"/>
    <dbReference type="PhylomeDB" id="Q58476"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000184674" description="Uncharacterized ATP-binding protein MJ1076">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="337" mass="40093" checksum="D83CAF90FA432B81" modified="1997-11-01" version="1">MRFYNREKELNYLKNYVQLEPNSILFVYGPKSSGKSTVMMRVIKELENSNIVFFYYNLRKYATPTKDEFLSIFFEKSDKKYLLNKLEINLKIFKFGIEENFDFNNIKLNDVFAKINESINTVIKDGKRPVLVIDELQKLKNIYFNSGKSLLNELFNLFVSLTKMEHLCHVICLTSDTLFIDNVYRNSSLSEASEYYLIDWLKKDDIKKILKEEGFNKKEIDYCLNYLSLPYEISQLINNKKLGLSVEETIKRWINIEADGIKYLIDTSDLNEEEIYKVLSKFKDKIKINYKKDVKKEEMKYIKFLIENEILFYDVINGIIKPTSVKKWYAIKEILDK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58494</accession>
    <name>Y1094_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1094</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1094</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64436">
        <property type="entry name" value="E64436"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870606.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6PE3">
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
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            <begin position="376"/>
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    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00999"/>
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    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="6PEU"/>
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    </evidence>
    <sequence length="385" mass="44159" checksum="22E404017BE43946" modified="2001-04-27" version="3">MDIKYKLASYRICSPEETFEKIQEALKKIETVEIKNIQHLDKVNIPVYYLKRRVVVDGKEGIAIHYGKGANDIQAKVSACMEAIERFSASYDKNKVKEKPDNPINVEDLILPQYADKNVKEWVEGIDIINNETIDVPADAVFYPTSGKLFRGNTNGLASGNNLDEAILHATLEIIERDAWSLADLARKIPTKINPEDAKNPLIHELIEKYEKAGVKIILKDLTSEFEIPVVAAISDDLSKNPLMLCVGVGCHLHPEIAILRALTEVAQSRASQLHGFRRDAKLREEFTSKIPYERLKRIHRKWFEFEGEINIADMPNNARYDLKKDLKFIKDKLSEFGFDKLIYVDLNKVGVDAVRVIIPKMEVYTIDRDRLSRRAFERVKKLYY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="141" xmlns="http://uniprot.org/uniprot">
    <accession>Q58504</accession>
    <name>KHSE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Homoserine kinase</fullName>
            <shortName>HK</shortName>
            <shortName>HSK</shortName>
            <ecNumber>2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">thrB</name>
        <name type="ordered locus">MJ1104</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Structure" volume="8" first="1247" last="1257">
            <title>Structure and mechanism of homoserine kinase: prototype for the GHMP kinase superfamily.</title>
            <authorList>
                <person name="Zhou T."/>
                <person name="Daugherty M."/>
                <person name="Grishin N.V."/>
                <person name="Osterman A.L."/>
                <person name="Zhang H."/>
            </authorList>
            <dbReference type="PubMed" id="11188689"/>
            <dbReference type="DOI" id="10.1016/S0969-2126(00)00533-5"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + L-homoserine = ADP + H(+) + O-phospho-L-homoserine</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99107" version="1"/>
        </conflict>
    </comment>
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        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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        <property type="resolution" value="2.10 A"/>
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        <property type="resolution" value="2.25 A"/>
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        <property type="resolution" value="1.80 A"/>
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        <property type="resolution" value="1.90 A"/>
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        <property type="reaction ID" value="UER00064"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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    </dbReference>
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        <property type="entry name" value="SSF55060"/>
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    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0791">Threonine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000156641" description="Homoserine kinase">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="86"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="2"/>
            <end position="12"/>
        </location>
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    <feature type="helix" evidence="4">
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            <begin position="15"/>
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            <begin position="18"/>
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        <location>
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        <location>
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    <feature type="strand" evidence="4">
        <location>
            <begin position="89"/>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="93"/>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="115"/>
            <end position="130"/>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="138"/>
            <end position="143"/>
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    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="145"/>
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    <feature type="turn" evidence="4">
        <location>
            <begin position="152"/>
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    <feature type="strand" evidence="4">
        <location>
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    <feature type="strand" evidence="4">
        <location>
            <begin position="168"/>
            <end position="171"/>
        </location>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="179"/>
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    <feature type="strand" evidence="5">
        <location>
            <begin position="188"/>
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            <begin position="214"/>
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    <feature type="helix" evidence="4">
        <location>
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        </location>
    </feature>
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        <location>
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        <location>
            <begin position="246"/>
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        <location>
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    <feature type="helix" evidence="4">
        <location>
            <begin position="270"/>
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    <feature type="strand" evidence="4">
        <location>
            <begin position="284"/>
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        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="293"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="1H72"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1H74"/>
        </source>
    </evidence>
    <sequence length="296" mass="32259" checksum="49919E68F3834C78" modified="2001-06-01" version="2">MKVRVKAPCTSANLGVGFDVFGLCLKEPYDVIEVEAIDDKEIIIEVDDKNIPTDPDKNVAGIVAKKMIDDFNIGKGVKITIKKGVKAGSGLGSSAASSAGTAYAINELFKLNLDKLKLVDYASYGELASSGAKHADNVAPAIFGGFTMVTNYEPLEVLHIPIDFKLDILIAIPNISINTKEAREILPKAVGLKDLVNNVGKACGMVYALYNKDKSLFGRYMMSDKVIEPVRGKLIPNYFKIKEEVKDKVYGITISGSGPSIIAFPKEEFIDEVENILRDYYENTIRTEVGKGVEVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-08" modified="2022-08-03" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q58508</accession>
    <name>SYFB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="1">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="1">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">pheT</name>
        <name type="ordered locus">MJ1108</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + H(+) + L-phenylalanyl-tRNA(Phe)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64438">
        <property type="entry name" value="C64438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870620.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58508"/>
    <dbReference type="SMR" id="Q58508"/>
    <dbReference type="STRING" id="243232.MJ_1108"/>
    <dbReference type="EnsemblBacteria" id="AAB99110">
        <property type="protein sequence ID" value="AAB99110"/>
        <property type="gene ID" value="MJ_1108"/>
    </dbReference>
    <dbReference type="GeneID" id="1452005"/>
    <dbReference type="KEGG" id="mja:MJ_1108"/>
    <dbReference type="eggNOG" id="arCOG00412">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020279_3_0_2"/>
    <dbReference type="InParanoid" id="Q58508"/>
    <dbReference type="OMA" id="FPGRCAN"/>
    <dbReference type="OrthoDB" id="55024at2157"/>
    <dbReference type="PhylomeDB" id="Q58508"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PTHR10947"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="SSF46955"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="SSF55681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000127002" description="Phenylalanine--tRNA ligase beta subunit">
        <location>
            <begin position="1"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="B5" evidence="1">
        <location>
            <begin position="275"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="328"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="548" mass="62697" checksum="4EE8DE2CD93BB0DA" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-28" modified="2022-05-25" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58513</accession>
    <name>Y1113_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative glycosyltransferase MJ1113</fullName>
            <ecNumber>2.-.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1113</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 4 family.</text>
    </comment>
    <dbReference type="EC" id="2.-.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64438">
        <property type="entry name" value="H64438"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870624.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58513"/>
    <dbReference type="SMR" id="Q58513"/>
    <dbReference type="STRING" id="243232.MJ_1113"/>
    <dbReference type="EnsemblBacteria" id="AAB99115">
        <property type="protein sequence ID" value="AAB99115"/>
        <property type="gene ID" value="MJ_1113"/>
    </dbReference>
    <dbReference type="GeneID" id="1452009"/>
    <dbReference type="KEGG" id="mja:MJ_1113"/>
    <dbReference type="eggNOG" id="arCOG03199">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023982_4_0_2"/>
    <dbReference type="InParanoid" id="Q58513"/>
    <dbReference type="OMA" id="DDILGWK"/>
    <dbReference type="OrthoDB" id="88899at2157"/>
    <dbReference type="PhylomeDB" id="Q58513"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008963">
        <property type="term" value="F:phospho-N-acetylmuramoyl-pentapeptide-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PTHR22926"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000108952" description="Putative glycosyltransferase MJ1113">
        <location>
            <begin position="1"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="2"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="280"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="301" mass="33363" checksum="B94D45ED1EED81A5" modified="1996-11-01" version="1">MGHYFINLFTYTIIAFIFSAVLCKFLMKKMINYKFGYDLHKKEKIKVPEMGGLAVLFSNALFIPFVNPIFVLPIITAGIIGIVDDIAKLSPKEKLILLFISGLIIGILFYNNSYVNLIEILIIALGIMISSNLTNMLAGFNGLEIGMGVIASISLALVLFLDNYTTGFLSALIFSASYLGLLIFNKYPAKVFPGDVGTLPIGAFLAVLAVVYKEYIPFLVIMMPYVIDASLKYLSAGVMSRDEHKPTTLKEDGKLYYIGGYLSLPRLILKYKPMREPHLVTVLWIIGIFFGIVGILISLIA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58519</accession>
    <name>DAPF_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Diaminopimelate epimerase</fullName>
            <shortName evidence="1">DAP epimerase</shortName>
            <ecNumber evidence="1">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">dapF</name>
        <name type="ordered locus">MJ1119</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,6S)-2,6-diaminoheptanedioate = meso-2,6-diaminoheptanedioate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64439">
        <property type="entry name" value="F64439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870630.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58519"/>
    <dbReference type="SMR" id="Q58519"/>
    <dbReference type="STRING" id="243232.MJ_1119"/>
    <dbReference type="EnsemblBacteria" id="AAB99120">
        <property type="protein sequence ID" value="AAB99120"/>
        <property type="gene ID" value="MJ_1119"/>
    </dbReference>
    <dbReference type="GeneID" id="1452015"/>
    <dbReference type="KEGG" id="mja:MJ_1119"/>
    <dbReference type="eggNOG" id="arCOG02255">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053306_3_0_2"/>
    <dbReference type="InParanoid" id="Q58519"/>
    <dbReference type="OMA" id="HVAMRVH"/>
    <dbReference type="OrthoDB" id="61864at2157"/>
    <dbReference type="PhylomeDB" id="Q58519"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="PTHR31689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0457">Lysine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149886" description="Diaminopimelate epimerase">
        <location>
            <begin position="1"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="237"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="77"/>
            <end position="78"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="228"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="1">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="1">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <sequence length="295" mass="33442" checksum="8CB87BF46806982A" modified="1996-11-01" version="1">MEFTKMHALGNDYIVINEFDGEKVKEEEKAEFSRKICRRGFSVGADGVIFIQKPTSDEYDVRFRIFNSDGSEAEMCGNGIRCFSKYVYERIMKKNPLKVETKGGLRVSEMEIEGDEVKKIKVYMGVPKFKLKDIPMVVDGYKEDDEFLNGELKLKNPYLPKVKLSVVNVGNPHAVIFVEDNNIDLDFVREHLDVIGKEIEHHEAFPERINVHFVKVLNPNEIRIVTWERGAGYTTACGTGTTASVIMAHKLGKTNNRVLAHLDGGDLEIEIKDDGVYMIGDAVMVYDAKLINIGW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58550</accession>
    <name>Y1150_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein MJ1150</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1150</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64443">
        <property type="entry name" value="E64443"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58550"/>
    <dbReference type="SMR" id="Q58550"/>
    <dbReference type="STRING" id="243232.MJ_1150"/>
    <dbReference type="EnsemblBacteria" id="AAB99150">
        <property type="protein sequence ID" value="AAB99150"/>
        <property type="gene ID" value="MJ_1150"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1150"/>
    <dbReference type="eggNOG" id="arCOG00928">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095956_1_0_2"/>
    <dbReference type="InParanoid" id="Q58550"/>
    <dbReference type="OMA" id="LRLAHMI"/>
    <dbReference type="PhylomeDB" id="Q58550"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="DUF99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="PTHR39518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="DUF99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000149233" description="UPF0215 protein MJ1150">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <sequence length="184" mass="21384" checksum="121B036339B7BE15" modified="1996-11-01" version="1">MVLMKDEVEVIGFDDAPFNKADKVCILIGTYMRGNRIIDGIYFRKFKKDGMDVTEKIIDIVKEKHYKKIKVIFLAGITFGGFNIADLWEINKETEKPVIVVIDKYPNKEKIFLALKKYFDDADERIKLINSFPEPEKMENIYVQYVGADKEFVKNVIKKTKLKSKIPECLRISHLIGRGFLGLR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q58572</accession>
    <name>RELB4_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative antitoxin RelB4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">relB4</name>
        <name type="ordered locus">MJ1172</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2005" name="Nucleic Acids Res." volume="33" first="966" last="976">
            <title>Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes.</title>
            <authorList>
                <person name="Pandey D.P."/>
                <person name="Gerdes K."/>
            </authorList>
            <dbReference type="PubMed" id="15718296"/>
            <dbReference type="DOI" id="10.1093/nar/gki201"/>
        </citation>
        <scope>POSSIBLE FUNCTION</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Antitoxin component of a type II toxin-antitoxin (TA) system. Its cognate toxin is RelE4.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64446">
        <property type="entry name" value="C64446"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58572"/>
    <dbReference type="SMR" id="Q58572"/>
    <dbReference type="STRING" id="243232.MJ_1172"/>
    <dbReference type="EnsemblBacteria" id="AAB99187">
        <property type="protein sequence ID" value="AAB99187"/>
        <property type="gene ID" value="MJ_1172"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1172"/>
    <dbReference type="eggNOG" id="arCOG05071">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_189630_0_0_2"/>
    <dbReference type="InParanoid" id="Q58572"/>
    <dbReference type="OMA" id="INVNERT"/>
    <dbReference type="PhylomeDB" id="Q58572"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020271">
        <property type="entry name" value="Uncharacterised_MJ1172"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10884">
        <property type="entry name" value="DUF2683"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107199" description="Putative antitoxin RelB4">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="84" mass="9986" checksum="4B7837421CBADAE4" modified="1996-11-01" version="1">MILMVKAIVDITDENNRIINIVKAKYNLRDKSQAINKIIEEYAEFLLEDELKPEYIEKIRNIMKNEKPIYIGSIENLKKRYLGE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58582</accession>
    <name>Y1182_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1182</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1182</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64447">
        <property type="entry name" value="E64447"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870695.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58582"/>
    <dbReference type="SMR" id="Q58582"/>
    <dbReference type="STRING" id="243232.MJ_1182"/>
    <dbReference type="PRIDE" id="Q58582"/>
    <dbReference type="EnsemblBacteria" id="AAB99183">
        <property type="protein sequence ID" value="AAB99183"/>
        <property type="gene ID" value="MJ_1182"/>
    </dbReference>
    <dbReference type="GeneID" id="1452080"/>
    <dbReference type="KEGG" id="mja:MJ_1182"/>
    <dbReference type="eggNOG" id="arCOG00570">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_777578_0_0_2"/>
    <dbReference type="InParanoid" id="Q58582"/>
    <dbReference type="OMA" id="CAYYKND"/>
    <dbReference type="OrthoDB" id="31233at2157"/>
    <dbReference type="PhylomeDB" id="Q58582"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="SSF51905"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107205" description="Uncharacterized protein MJ1182">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <sequence length="366" mass="42216" checksum="FECB31A9BE771A51" modified="1996-11-01" version="1">MQVCIIGAGLSGSILYRLLSEDGFYINIYDHVLVRGCKSMNFIFSNKNEILTVKKVLKTVNINIKDYIIREIKEVNIGGDNYYPNKKIYVINKSKLIEDLVPRTVVTNREFNPVIRRYTTKVIDTGVIVREFNTEAETKFYDLVVDASGCAKVLQLGNVYDKYKNDIKTCQFLIAYENEESPEKFDKFFIDEIKIHKGKPMIGYTWITPIDDGLYHVGCAYYKNDHELWTYLTKYTKKMFGSDYVRVCGCTSKINGNLISESFIGGIYEKRCVAGVGESIGLTTPLGHGNIYAIISAYILSRFIKKYDLNEAVLKYKDYIPKKFAELDKEKKAVRNFNVLRITKLLRDYYNIPAHESMKIILKSLY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-08-03" version="133" xmlns="http://uniprot.org/uniprot">
    <accession>Q58632</accession>
    <name>RBL_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribulose bisphosphate carboxylase</fullName>
            <shortName evidence="1">RuBisCO</shortName>
            <ecNumber evidence="1">4.1.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rbcL</name>
        <name type="ordered locus">MJ1235</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1999" name="J. Bacteriol." volume="181" first="1569" last="1575">
            <title>Unusual ribulose 1,5-bisphosphate carboxylase/oxygenase of anoxic Archaea.</title>
            <authorList>
                <person name="Watson G.M.F."/>
                <person name="Yu J.-P."/>
                <person name="Tabita F.R."/>
            </authorList>
            <dbReference type="PubMed" id="10049390"/>
        </citation>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Bacteriol." volume="185" first="3049" last="3059">
            <title>Synthesis of catalytically active form III ribulose 1,5-bisphosphate carboxylase/oxygenase in archaea.</title>
            <authorList>
                <person name="Finn M.W."/>
                <person name="Tabita F.R."/>
            </authorList>
            <dbReference type="PubMed" id="12730164"/>
            <dbReference type="DOI" id="10.1128/JB.185.10.3049-3059.2003"/>
        </citation>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the addition of molecular CO(2) and H(2)O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>2 (2R)-3-phosphoglycerate + 2 H(+) = CO2 + D-ribulose 1,5-bisphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:23124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="4.1.1.39"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate + 2-phosphoglycolate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36631"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57870"/>
            <dbReference type="ChEBI" id="CHEBI:58033"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2 3">Reversibly inhibited by O(2).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 65 degrees Celsius. Highly thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2 3">Homodimer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O(2) to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RuBisCO large chain family. Type III subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.39" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870747.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58632"/>
    <dbReference type="SMR" id="Q58632"/>
    <dbReference type="STRING" id="243232.MJ_1235"/>
    <dbReference type="DNASU" id="1452131"/>
    <dbReference type="EnsemblBacteria" id="AAB99239">
        <property type="protein sequence ID" value="AAB99239"/>
        <property type="gene ID" value="MJ_1235"/>
    </dbReference>
    <dbReference type="GeneID" id="1452131"/>
    <dbReference type="KEGG" id="mja:MJ_1235"/>
    <dbReference type="eggNOG" id="arCOG04443">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031450_3_1_2"/>
    <dbReference type="InParanoid" id="Q58632"/>
    <dbReference type="OMA" id="IHGHPDG"/>
    <dbReference type="OrthoDB" id="9533at2157"/>
    <dbReference type="PhylomeDB" id="Q58632"/>
    <dbReference type="BRENDA" id="4.1.1.39">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016984">
        <property type="term" value="F:ribulose-bisphosphate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015977">
        <property type="term" value="P:carbon fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08213">
        <property type="entry name" value="RuBisCO_large_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01133">
        <property type="entry name" value="RuBisCO_L_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033966">
        <property type="entry name" value="RuBisCO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017712">
        <property type="entry name" value="RuBisCO_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000685">
        <property type="entry name" value="RuBisCO_lsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036376">
        <property type="entry name" value="RuBisCO_lsu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017443">
        <property type="entry name" value="RuBisCO_lsu_fd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036422">
        <property type="entry name" value="RuBisCO_lsu_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42704">
        <property type="entry name" value="PTHR42704"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00016">
        <property type="entry name" value="RuBisCO_large"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02788">
        <property type="entry name" value="RuBisCO_large_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00014">
        <property type="entry name" value="RuBisCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51649">
        <property type="entry name" value="SSF51649"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54966">
        <property type="entry name" value="SSF54966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03326">
        <property type="entry name" value="rubisco_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0120">Carbon dioxide fixation</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000062673" description="Ribulose bisphosphate carboxylase">
        <location>
            <begin position="1"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="1">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="181"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="353"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="375"/>
            <end position="378"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="1">
        <location>
            <position position="309"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="1">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10049390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12730164"/>
        </source>
    </evidence>
    <sequence length="425" mass="47859" checksum="C6F9F1653BA76039" modified="1998-06-01" version="2">MDYINLNYRPNEGDLLSCMVIKGENLEKLANEIAGESSIGTWTKVQTMKSDIYEKLRPKVYEIKEIGEENGYKVGLIKIAYPLYDFEINNMPGVLAGIAGNIFGMKIAKGLRILDFRFPAEFVKAYKGPRFGIEGVRETLKIKERPLLGTIVKPKVGLKTEEHAKVAYEAWVGGVDLVKDDENLTSQEFNKFEDRIYKTLEMRDKAEEETGERKAYMPNITAPYREMIRRAEIAEDAGSEYVMIDVVVCGFSAVQSFREEDFKFIIHAHRAMHAAMTRSRDFGISMLALAKIYRLLGVDQLHIGTVVGKMEGGEKEVKAIRDEIVYDKVEADNENKFFNQDWFDIKPVFPVSSGGVHPRLVPKIVEILGRDLIIQAGGGVHGHPDGTRAGAKAMRAAIEAIIEGKSLEEKAEEVAELKKALEYWK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q58634</accession>
    <name>PSB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Proteasome subunit beta</fullName>
            <ecNumber evidence="1">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">psmB</name>
        <name type="ordered locus">MJ1237</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="J. Bacteriol." volume="182" first="1680" last="1692">
            <title>Biochemical and physical properties of the Methanococcus jannaschii 20S proteasome and PAN, a homolog of the ATPase (Rpt) subunits of the eucaryal 26S proteasome.</title>
            <authorList>
                <person name="Wilson H.L."/>
                <person name="Ou M.S."/>
                <person name="Aldrich H.C."/>
                <person name="Maupin-Furlow J."/>
            </authorList>
            <dbReference type="PubMed" id="10692374"/>
            <dbReference type="DOI" id="10.1128/JB.182.6.1680-1692.2000"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBUNIT</scope>
        <scope>INTERACTION WITH PAN</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2009" name="Mol. Cell" volume="34" first="473" last="484">
            <title>Structural insights into the regulatory particle of the proteasome from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Zhang F."/>
                <person name="Hu M."/>
                <person name="Tian G."/>
                <person name="Zhang P."/>
                <person name="Finley D."/>
                <person name="Jeffrey P.D."/>
                <person name="Shi Y."/>
            </authorList>
            <dbReference type="PubMed" id="19481527"/>
            <dbReference type="DOI" id="10.1016/j.molcel.2009.04.021"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS) IN COMPLEX WITH ALPHA SUBUNIT</scope>
        <scope>GATED STRUCTURE</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation. The M.jannaschii proteasome is able to cleave oligopeptides after Glu, Asp, Tyr, Phe, Trp, slightly after Arg, but not after Ala. Thus, displays caspase-like and chymotrypsin-like activities and low level of trypsin-like activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 3">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64454">
        <property type="entry name" value="D64454"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870749.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3H4P">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.10 A"/>
        <property type="chains" value="a/b/c/d/e/f/g/h/i/j/k/l/m/n=7-224"/>
    </dbReference>
    <dbReference type="PDBsum" id="3H4P"/>
    <dbReference type="AlphaFoldDB" id="Q58634"/>
    <dbReference type="SMR" id="Q58634"/>
    <dbReference type="STRING" id="243232.MJ_1237"/>
    <dbReference type="MEROPS" id="T01.002"/>
    <dbReference type="EnsemblBacteria" id="AAB99241">
        <property type="protein sequence ID" value="AAB99241"/>
        <property type="gene ID" value="MJ_1237"/>
    </dbReference>
    <dbReference type="GeneID" id="1452133"/>
    <dbReference type="KEGG" id="mja:MJ_1237"/>
    <dbReference type="eggNOG" id="arCOG00970">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_7_2_2"/>
    <dbReference type="InParanoid" id="Q58634"/>
    <dbReference type="OMA" id="VDKTGPH"/>
    <dbReference type="OrthoDB" id="89767at2157"/>
    <dbReference type="PhylomeDB" id="Q58634"/>
    <dbReference type="EvolutionaryTrace" id="Q58634"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005839">
        <property type="term" value="C:proteasome core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="CDD" id="cd03764">
        <property type="entry name" value="proteasome_beta_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-0647">Proteasome</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0888">Threonine protease</keyword>
    <keyword id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_0000026661" description="Removed in mature form; by autocatalysis" evidence="1">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000026662" description="Proteasome subunit beta">
        <location>
            <begin position="7"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="10692374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="19481527"/>
        </source>
    </evidence>
    <sequence length="224" mass="24285" checksum="45B2A0D1EAEF7A58" modified="1997-11-01" version="1" precursor="true">MDVMKGTTTVGLICDDAVILATDKRASLGNLVADKEAKKLYKIDDYIAMTIAGSVGDAQAIVRLLIAEAKLYKMRTGRNIPPLACATLLSNILHSSRMFPFLTQIIIGGYDLLEGAKLFSLDPLGGMNEEKTFTATGSGSPIAYGVLEAGYDRDMSVEEGIKLALNALKSAMERDTFSGNGISLAVITKDGVKIFEDEEIEKILDSMKAKPKKKTTKRSRRKSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-06-06" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58649</accession>
    <name>Y1251_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0254 protein MJ1251</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1251</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0254 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64456">
        <property type="entry name" value="C64456"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870765.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3WVA">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.40 A"/>
        <property type="chains" value="A/B=1-167"/>
    </dbReference>
    <dbReference type="PDB" id="3WVB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A/B=1-167"/>
    </dbReference>
    <dbReference type="PDB" id="3WVC">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.00 A"/>
        <property type="chains" value="A/B=1-167"/>
    </dbReference>
    <dbReference type="PDBsum" id="3WVA"/>
    <dbReference type="PDBsum" id="3WVB"/>
    <dbReference type="PDBsum" id="3WVC"/>
    <dbReference type="AlphaFoldDB" id="Q58649"/>
    <dbReference type="SMR" id="Q58649"/>
    <dbReference type="STRING" id="243232.MJ_1251"/>
    <dbReference type="EnsemblBacteria" id="AAB99254">
        <property type="protein sequence ID" value="AAB99254"/>
        <property type="gene ID" value="MJ_1251"/>
    </dbReference>
    <dbReference type="GeneID" id="1452150"/>
    <dbReference type="KEGG" id="mja:MJ_1251"/>
    <dbReference type="eggNOG" id="arCOG04865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1451416_0_0_2"/>
    <dbReference type="OMA" id="ECFTHGK"/>
    <dbReference type="OrthoDB" id="81238at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00673">
        <property type="entry name" value="UPF0254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009625">
        <property type="entry name" value="HcgF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06787">
        <property type="entry name" value="HcgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018786">
        <property type="entry name" value="UPF0254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000147367" description="UPF0254 protein MJ1251">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="2"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="10"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="32"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="37"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="46"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="57"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="72"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="76"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="104"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="112"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="117"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="126"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="137"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="143"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="3WVA"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="3WVC"/>
        </source>
    </evidence>
    <sequence length="167" mass="18946" checksum="B41FFDCFFEA89F86" modified="1996-11-01" version="1">MITVATAECFTHANIGLTIHKAAAGYEDFEFKYLFSEEDLKLMKNVRVISAMFVPSIIGVEKLLDIKLPEPDFNYKYAKAYSEEKDLEVAKLMAEGLKKKLNVNISIGSTAGVGRGAICILTDNNRYLFTSDVYANLITFENIKERQKNGIEKGIKRFLEILKKEYF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q58667</accession>
    <name>HACB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methanogen homoaconitase small subunit</fullName>
            <shortName>HACN</shortName>
            <ecNumber evidence="2">4.2.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hacB</name>
        <name type="ordered locus">MJ1271</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="J. Bacteriol." volume="189" first="4391" last="4400">
            <title>Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Drevland R.M."/>
                <person name="Waheed A."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="17449626"/>
            <dbReference type="DOI" id="10.1128/JB.00166-07"/>
        </citation>
        <scope>PUTATIVE FUNCTION AS A HOMOACONITASE</scope>
        <scope>LACK OF FUNCTION AS A ISOPROPYLMALATE ISOMERASE</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Biol. Chem." volume="283" first="28888" last="28896">
            <title>Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.</title>
            <authorList>
                <person name="Drevland R.M."/>
                <person name="Jia Y."/>
                <person name="Palmer D.R.J."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="18765671"/>
            <dbReference type="DOI" id="10.1074/jbc.M802159200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="Biochemistry" volume="49" first="2687" last="2696">
            <title>Substrate specificity determinants of the methanogen homoaconitase enzyme: structure and function of the small subunit.</title>
            <authorList>
                <person name="Jeyakanthan J."/>
                <person name="Drevland R.M."/>
                <person name="Gayathri D.R."/>
                <person name="Velmurugan D."/>
                <person name="Shinkai A."/>
                <person name="Kuramitsu S."/>
                <person name="Yokoyama S."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="20170198"/>
            <dbReference type="DOI" id="10.1021/bi901766z"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS)</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>MUTAGENESIS OF ARG-26 AND THR-27</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Component of a hydro-lyase with broad substrate specificity for cis-unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4-tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)-aconitate, and even the non-physiological cis-homo(4)-aconitate with similar efficiency. These reactions are part of the biosynthesis pathway of coenzyme B. Can also catalyze the hydration of maleate to (R)-malate, and that of cis-aconitate. Cannot catalyze the hydration of citraconate and the dehydration of (S)-homocitrate, citramalate, 2-isopropylmalate, 3-isopropylmalate, citrate or threo-DL-isocitrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R)-homocitrate = (2R,3S)-homoisocitrate</text>
            <dbReference type="Rhea" id="RHEA:32303"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:32304"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R)-homocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:26101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:26102"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(2R,3S)-homoisocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:15487"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>cis-(homo)2aconitate + H2O = (2R,3S)-iso(homo)2citrate</text>
            <dbReference type="Rhea" id="RHEA:68416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:72710"/>
            <dbReference type="ChEBI" id="CHEBI:72722"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:68417"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>cis-(homo)3aconitate + H2O = (2R,3S)-iso(homo)3citrate</text>
            <dbReference type="Rhea" id="RHEA:68420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:72712"/>
            <dbReference type="ChEBI" id="CHEBI:177881"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:68421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>(R)-malate = H2O + maleate</text>
            <dbReference type="Rhea" id="RHEA:23692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:30780"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cis-aconitate + H2O = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:22144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16383"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">22 uM for cis-homoaconitate (at 60 degrees Celsius)</KM>
            <KM evidence="2">30 uM for cis-homo(2)-aconitate (at 60 degrees Celsius)</KM>
            <KM evidence="2">36 uM for cis-homo(3)-aconitate (at 60 degrees Celsius)</KM>
            <KM evidence="2">175 uM for cis-homo(4)-aconitate (at 60 degrees Celsius)</KM>
            <KM evidence="2">330 uM for maleate (at 60 degrees Celsius)</KM>
            <KM>1500 uM for (R)-homocitrate (at 60 degrees Celsius)</KM>
            <KM evidence="3">300 uM for cis-aconitate</KM>
            <Vmax evidence="2">0.68 umol/min/mg enzyme for cis-homoaconitate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.6 umol/min/mg enzyme for cis-homo(2)aconitate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="2">2.2 umol/min/mg enzyme for cis-homo(3)aconitate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="2">5.1 umol/min/mg enzyme for cis-homo(4)aconitate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="2">5.5 umol/min/mg enzyme for maleate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.59 umol/min/mg enzyme for (R)-homocitrate dehydration reaction (at 60 degrees Celsius)</Vmax>
            <text evidence="3">kcat is 0.75 sec(-1) for cis-aconitate hydration reaction (PubMed:20170198). Kinetic parameters measured using the HacAB complex.</text>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 9. Active from pH 8 to 10.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text>Organic acid metabolism; 2-oxosuberate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of 2 HacA and 2 HacB proteins. Cannot form a complex with LeuC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.114" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64458">
        <property type="entry name" value="F64458"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870784.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PKP">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=1-170"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PKP"/>
    <dbReference type="AlphaFoldDB" id="Q58667"/>
    <dbReference type="SMR" id="Q58667"/>
    <dbReference type="STRING" id="243232.MJ_1271"/>
    <dbReference type="EnsemblBacteria" id="AAB99277">
        <property type="protein sequence ID" value="AAB99277"/>
        <property type="gene ID" value="MJ_1271"/>
    </dbReference>
    <dbReference type="GeneID" id="1452169"/>
    <dbReference type="KEGG" id="mja:MJ_1271"/>
    <dbReference type="eggNOG" id="arCOG02230">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081378_1_1_2"/>
    <dbReference type="InParanoid" id="Q58667"/>
    <dbReference type="OMA" id="APFMVGE"/>
    <dbReference type="OrthoDB" id="86621at2157"/>
    <dbReference type="PhylomeDB" id="Q58667"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-2005"/>
    <dbReference type="SABIO-RK" id="Q58667"/>
    <dbReference type="UniPathway" id="UPA00919"/>
    <dbReference type="EvolutionaryTrace" id="Q58667"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0000315"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052633">
        <property type="term" value="F:isocitrate hydro-lyase (cis-aconitate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050075">
        <property type="term" value="F:maleate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="CAFA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
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    <dbReference type="DisProt" id="DP00619"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
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    </dbReference>
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        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
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        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
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        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
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        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000141936" description="Methanogen homoaconitase small subunit">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="YLRT">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="site" description="Critical for substrate specificity">
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Creates a promiscuous enzyme with both HACN and IPMI activities.">
        <original>RT</original>
        <variation>VY</variation>
        <location>
            <begin position="26"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Allows the recognition of substrates of IPMI enzymes since it becomes able to efficiently catalyze citraconate hydration and 3-isopropylmalate dehydration. Largely decreases substrate affinity for cis-homo(1-3)-aconitate." evidence="3">
        <original>R</original>
        <variation>K</variation>
        <variation>V</variation>
        <location>
            <position position="26"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Largely decreases substrate affinity for cis-homo(1-3)-aconitate while slightly increases activity on these substrates, and also decreases substrate affinity for maleate. Gains the ability to hydrate citraconate, an IPMI substrate." evidence="3">
        <original>T</original>
        <variation>A</variation>
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="2"/>
            <end position="9"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="22"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="30"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="35"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="45"/>
            <end position="48"/>
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    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="54"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="59"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="63"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="68"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="76"/>
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        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="81"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="89"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="101"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="107"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="115"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="120"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="124"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="128"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="132"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="141"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="17449626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18765671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="20170198"/>
        </source>
    </evidence>
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    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="18765671"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="2PKP"/>
        </source>
    </evidence>
    <sequence length="170" mass="18665" checksum="58146C478777ECB7" modified="1996-11-01" version="1">MIIKGRAHKFGDDVDTDAIIPGPYLRTTDPYELASHCMAGIDENFPKKVKEGDVIVAGENFGCGSSREQAVIAIKYCGIKAVIAKSFARIFYRNAINVGLIPIIANTDEIKDGDIVEIDLDKEEIVITNKNKTIKCETPKGLEREILAAGGLVNYLKKRKLIQSKKGVKT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58676</accession>
    <name>Y1280_METJA</name>
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        <recommendedName>
            <fullName>Uncharacterized protein MJ1280</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1280</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="G64459"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870793.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58676"/>
    <dbReference type="SMR" id="Q58676"/>
    <dbReference type="STRING" id="243232.MJ_1280"/>
    <dbReference type="EnsemblBacteria" id="AAB99286">
        <property type="protein sequence ID" value="AAB99286"/>
        <property type="gene ID" value="MJ_1280"/>
    </dbReference>
    <dbReference type="GeneID" id="1452178"/>
    <dbReference type="KEGG" id="mja:MJ_1280"/>
    <dbReference type="HOGENOM" id="CLU_1674012_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107248" description="Uncharacterized protein MJ1280">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="3"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="157" mass="17732" checksum="1340F75DAEE5B530" modified="1996-11-01" version="1">MNLILTLLIIFAIIGLLILIFGIWLIKKLLFPKKKPKPYQVRHGSSSSKRGYGSHDVYHHHYVTKDVYVHDYGDDDIYDERGKKEGDKDDSLIDKAIAFGAGAVTGYGVAEYGEEIKEEVEDIVKEGEDIIDDIVEEAEDFVEDVVDDFDNGGDDDY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58681</accession>
    <name>Y1285_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1285</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1285</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
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            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58681"/>
    <dbReference type="STRING" id="243232.MJ_1285"/>
    <dbReference type="EnsemblBacteria" id="AAB99294">
        <property type="protein sequence ID" value="AAB99294"/>
        <property type="gene ID" value="MJ_1285"/>
    </dbReference>
    <dbReference type="GeneID" id="1452185"/>
    <dbReference type="KEGG" id="mja:MJ_1285"/>
    <dbReference type="eggNOG" id="arCOG08270">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1465125_0_0_2"/>
    <dbReference type="OMA" id="KEFLMYY"/>
    <dbReference type="OrthoDB" id="85295at2157"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
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        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107253" description="Uncharacterized protein MJ1285">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="184" mass="21715" checksum="1C1605916DC36DE4" modified="1996-11-01" version="1">MELDYLLATAMFLIVCVYVISETVNLHSVYDIEEAKKEFLMYYNDLKYNYSISKGDLIFNFKVNKIGYVIEGFVFKDTSESRELIKYLENLNGSYIIAYSPSKDEFIITKNHEFLRIIGHYNISAKYKKGEYGDIEIIYPKNYSINYREFQGISCNKLFEVPFYIVDKNENITLKYYGILEVGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="111" xmlns="http://uniprot.org/uniprot">
    <accession>Q58715</accession>
    <name>Y1319_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized sodium-dependent transporter MJ1319</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1319</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Putative sodium-dependent transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location>Cell membrane</location>
            <topology>Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64464">
        <property type="entry name" value="F64464"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58715"/>
    <dbReference type="SMR" id="Q58715"/>
    <dbReference type="STRING" id="243232.MJ_1319"/>
    <dbReference type="TCDB" id="2.A.22.5.1">
        <property type="family name" value="the neurotransmitter:sodium symporter (nss) family"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99329">
        <property type="protein sequence ID" value="AAB99329"/>
        <property type="gene ID" value="MJ_1319"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1319"/>
    <dbReference type="eggNOG" id="arCOG04466">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006855_3_3_2"/>
    <dbReference type="InParanoid" id="Q58715"/>
    <dbReference type="OMA" id="NLWGFAY"/>
    <dbReference type="PhylomeDB" id="Q58715"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000175">
        <property type="entry name" value="Na/ntran_symport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037272">
        <property type="entry name" value="SNS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11616">
        <property type="entry name" value="PTHR11616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SSF161070"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00610">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0769">Symport</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000214820" description="Uncharacterized sodium-dependent transporter MJ1319">
        <location>
            <begin position="1"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="13"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="359"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="428"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="492" mass="53468" checksum="A4CEF33AC40D4DB9" modified="1996-11-01" version="1">MSYMERESWSSNLGFILASVGSAIGLGNIWRFGYMVYTNGGGAFLIPYIVALLCVGIPLMILEFAIGHYTKKSAPLALEKLHKGSEWTGWFAVISGFIITSYYVVIIAWCLYYLIILVIYGYPSDPNAYFFHNILQISSGVEDIGGVSYGILVSTLAVWGIVALILSAGIKNGLEKANKIMIPFLLFLIILLVLNALTLPGALTGIEWYLTPDFSALFNYNVWLSAFSQIFFSLSLGFGILIAYASYLPKKSDLTINAVTVSLLNCGFSFLAGFAVFGTLGYMSYTSGIPLDKAVSEGIGLAFVTFPKALSLLPFASRLFGIVFFLALVFAGISSAVSIVEASVSAIIDKFSLSRKKALLAVLALFIIISPIFTTGAGLYYLDIIDHFASGYLLPIAAILEIIIAIWLFGGDKLREHVNKLSEIKLGVWWKYLAGVVSPIILTAVVFLDASNVLTSGYGGYKTTYVIFGALIIPLAFVVSVILQKMKTIKGW</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-09-26" modified="2022-08-03" version="140" xmlns="http://uniprot.org/uniprot">
    <accession>Q58718</accession>
    <name>RAD50_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA double-strand break repair Rad50 ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rad50</name>
        <name type="ordered locus">MJ1322</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="2011-02" db="PDB data bank">
            <title>Crystal structure of the Mre11-Rad50-ATP S complex: understanding the interplay between Mre11 and Rad50.</title>
            <authorList>
                <person name="Lim H.S."/>
                <person name="Kim J.S."/>
                <person name="Park Y.B."/>
                <person name="Gwon G.H."/>
                <person name="Cho Y."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) IN COMPLEX WITH ADP</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="domain">
        <text evidence="1">The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SMC family. RAD50 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64465">
        <property type="entry name" value="A64465"/>
    </dbReference>
    <dbReference type="PDB" id="3AUX">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="A=1-190, A=825-1005"/>
    </dbReference>
    <dbReference type="PDB" id="3AUY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=11-190, A/B=825-1005"/>
    </dbReference>
    <dbReference type="PDB" id="3AV0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.10 A"/>
        <property type="chains" value="B=1-190, B=825-1005"/>
    </dbReference>
    <dbReference type="PDB" id="5DNY">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.11 A"/>
        <property type="chains" value="B/D=1-190, B/D=825-1005"/>
    </dbReference>
    <dbReference type="PDB" id="5F3W">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.11 A"/>
        <property type="chains" value="B/D=1-190, B/D=825-1005"/>
    </dbReference>
    <dbReference type="PDBsum" id="3AUX"/>
    <dbReference type="PDBsum" id="3AUY"/>
    <dbReference type="PDBsum" id="3AV0"/>
    <dbReference type="PDBsum" id="5DNY"/>
    <dbReference type="PDBsum" id="5F3W"/>
    <dbReference type="AlphaFoldDB" id="Q58718"/>
    <dbReference type="SMR" id="Q58718"/>
    <dbReference type="STRING" id="243232.MJ_1322"/>
    <dbReference type="PRIDE" id="Q58718"/>
    <dbReference type="EnsemblBacteria" id="AAB99331">
        <property type="protein sequence ID" value="AAB99331"/>
        <property type="gene ID" value="MJ_1322"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1322"/>
    <dbReference type="eggNOG" id="arCOG00368">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004785_0_2_2"/>
    <dbReference type="InParanoid" id="Q58718"/>
    <dbReference type="OMA" id="FELPYSH"/>
    <dbReference type="PhylomeDB" id="Q58718"/>
    <dbReference type="EvolutionaryTrace" id="Q58718"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03240">
        <property type="entry name" value="ABC_Rad50"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00449">
        <property type="entry name" value="RAD50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045171">
        <property type="entry name" value="ABC_Rad50"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022982">
        <property type="entry name" value="Rad50_ATPase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0175">Coiled coil</keyword>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000138654" description="DNA double-strand break repair Rad50 ATPase">
        <location>
            <begin position="1"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-hook" evidence="1">
        <location>
            <begin position="457"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="292"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="346"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="404"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="523"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="656"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="800"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 3">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 3">
        <location>
            <begin position="35"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 3">
        <location>
            <begin position="62"/>
            <end position="64"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="502"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="3"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="16"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="25"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="54"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="58"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="67"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="92"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="102"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="108"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
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        <location>
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        </location>
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        <location>
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        </location>
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        <location>
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        </location>
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    <feature type="strand" evidence="4">
        <location>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="262"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="269"/>
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        </location>
    </feature>
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        <location>
            <begin position="273"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="278"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="300"/>
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        </location>
    </feature>
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        <location>
            <begin position="307"/>
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        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="313"/>
            <end position="324"/>
        </location>
    </feature>
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        <location>
            <begin position="331"/>
            <end position="338"/>
        </location>
    </feature>
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        <location>
            <begin position="340"/>
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        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="347"/>
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00449"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="2">
        <source>
            <dbReference type="PDB" id="3AUX"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="3">
        <source>
            <dbReference type="PDB" id="3AUY"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3AUY"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="3AV0"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="5DNY"/>
        </source>
    </evidence>
    <sequence length="1005" mass="119388" checksum="9BBBB48173E788F3" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58728</accession>
    <name>Y1332_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized GTP-binding protein MJ1332</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1332</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.</text>
    </comment>
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        <property type="protein sequence ID" value="AAB99340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="C64466"/>
    </dbReference>
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    <dbReference type="STRING" id="243232.MJ_1332"/>
    <dbReference type="EnsemblBacteria" id="AAB99340">
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        <property type="gene ID" value="MJ_1332"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1332"/>
    <dbReference type="eggNOG" id="arCOG00357">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037276_1_0_2"/>
    <dbReference type="InParanoid" id="Q58728"/>
    <dbReference type="OMA" id="QIDCACA"/>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="MMR_HSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="TGS"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0342">GTP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000205446" description="Uncharacterized GTP-binding protein MJ1332">
        <location>
            <begin position="1"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="1">
        <location>
            <begin position="2"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="8"/>
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        </location>
        <ligand>
            <name>GTP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
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        </location>
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        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01228"/>
        </source>
    </evidence>
    <sequence length="393" mass="44267" checksum="5213CD351907BEC5" modified="1996-11-01" version="1">MAMIGLVGKPNVGKSTMFNALTEKPAEIGNYPFTTIQPNKGIAYITSPCPCKELGVKCNPRNSKCIDGIRHIPVEVIDVAGLVPGAHEGRGMGNKFLDDLRQADAFILVVDASGKTDAEGNPTENYDPVEDVKFLLNEIDMWIYSILTKNWDKLARRAQQEKNIVKALKDQLSGLNIDEDDIKMAIRDMDESPIKWTEEDLLNLAKKLRKISKPMIIAANKADHPDAEKNIERLKKEFKDYIVIPTSAEIELALKRAEKAGIIKRKENDFEIIDESKVNEQMRRAFDYIKDFLKKYGGTGVQECINKAYFDLLDMIVVYPVEDENKFSDKQGNVLPDAFLVKKGSTARDLAYKVHTELGEKFIYAIDAKKKIRVGADYELKHNDIIKIVSAAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58769</accession>
    <name>Y1374_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1374</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1374</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="entry name" value="E64471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870891.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58769"/>
    <dbReference type="SMR" id="Q58769"/>
    <dbReference type="STRING" id="243232.MJ_1374"/>
    <dbReference type="EnsemblBacteria" id="AAB99381">
        <property type="protein sequence ID" value="AAB99381"/>
        <property type="gene ID" value="MJ_1374"/>
    </dbReference>
    <dbReference type="GeneID" id="1452277"/>
    <dbReference type="KEGG" id="mja:MJ_1374"/>
    <dbReference type="eggNOG" id="arCOG00499">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074581_0_0_2"/>
    <dbReference type="InParanoid" id="Q58769"/>
    <dbReference type="OMA" id="HCHPDHY"/>
    <dbReference type="OrthoDB" id="107971at2157"/>
    <dbReference type="PhylomeDB" id="Q58769"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107303" description="Uncharacterized protein MJ1374">
        <location>
            <begin position="1"/>
            <end position="266"/>
        </location>
    </feature>
    <sequence length="266" mass="29920" checksum="5B6831CA308F2426" modified="1996-11-01" version="1">MRVEIIFLGCGGGRWATITQKKATGGFRIHTNELRMHVDPGPGAIVRLNELKISPWRTNALFISHCHPDHYTDGEIIVEAITQGMTKKRGVFLGSLSVVEGFGEYEYVVSKYHQSKLEEVRVLYPGDSAELYDTTIKATHTKHGDPFGIGFRLSTIYGDIGYTSDTEFIPQLIEDFDGVRILIANIVRKKNERIKGHLCSNDAIDLINSMNKKPELLIMNHMGVKMTNPQIEAEYISQNTGIEVIPARLGLKVELLNGKYKYQLIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58775</accession>
    <name>Y1380_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative RNase MJ1380</fullName>
            <ecNumber evidence="2">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Putative toxin MJ1380</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1380</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probable toxic component of a putative type VII toxin-antitoxin (TA) system, probably an RNase. Probably neutralized by cognate antitoxin MJ1379. Neutralization may be due to AMPylation by antitoxin MJ1379.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer, probably forms a complex with cognate antitoxin MJ1379.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Modified by cognate antitoxin MJ1379; probably at least 2 successive AMPylation events occur on Tyr-83.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HepT RNase toxin family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="frameshift">
            <sequence resource="EMBL-CDS" id="AAB99390" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99390.1"/>
        <property type="status" value="ALT_FRAME"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64472">
        <property type="entry name" value="C64472"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58775"/>
    <dbReference type="SMR" id="Q58775"/>
    <dbReference type="STRING" id="243232.MJ_1380"/>
    <dbReference type="EnsemblBacteria" id="AAB99390">
        <property type="protein sequence ID" value="AAB99390"/>
        <property type="gene ID" value="MJ_1380"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1380"/>
    <dbReference type="eggNOG" id="arCOG05024">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_142825_4_0_2"/>
    <dbReference type="InParanoid" id="Q58775"/>
    <dbReference type="PhylomeDB" id="Q58775"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110001">
        <property type="term" value="C:toxin-antitoxin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008201">
        <property type="entry name" value="HepT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037038">
        <property type="entry name" value="HepT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01934">
        <property type="entry name" value="DUF86"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000158264" description="Putative RNase MJ1380">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="RX(4)HXY motif" evidence="1">
        <location>
            <begin position="76"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="81"/>
        </location>
    </feature>
    <feature type="modified residue" description="O-di-AMP-tyrosine" evidence="1">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="A0A0B0QJR1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8ECH6"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="109" mass="13058" checksum="CA967C92E5E41087" modified="2021-04-07" version="2">MSKRDVKAFLYDILESANDVIEFTKDIDYNEFINNKMIRYAVIRALEIIGEASRYINNDFREKFPNVPWKEMVGLRNILIHKYFGIDYILLWKIVKEDVPKIKKEVEIV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58780</accession>
    <name>TRM56_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1385</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aTrm56 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64472">
        <property type="entry name" value="H64472"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870902.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58780"/>
    <dbReference type="SMR" id="Q58780"/>
    <dbReference type="STRING" id="243232.MJ_1385"/>
    <dbReference type="EnsemblBacteria" id="AAB99395">
        <property type="protein sequence ID" value="AAB99395"/>
        <property type="gene ID" value="MJ_1385"/>
    </dbReference>
    <dbReference type="GeneID" id="1452288"/>
    <dbReference type="KEGG" id="mja:MJ_1385"/>
    <dbReference type="eggNOG" id="arCOG01857">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123709_0_0_2"/>
    <dbReference type="InParanoid" id="Q58780"/>
    <dbReference type="OMA" id="VVHLTMY"/>
    <dbReference type="OrthoDB" id="83050at2157"/>
    <dbReference type="PhylomeDB" id="Q58780"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00077">
        <property type="entry name" value="tRNA_methyltr_aTrm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="PTHR42197"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000146929" description="tRNA (cytidine(56)-2'-O)-methyltransferase">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="110"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="128"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00077"/>
        </source>
    </evidence>
    <sequence length="179" mass="20573" checksum="05A10B97B3303B31" modified="1996-11-01" version="1">MVVEVLRLGHRGDRDKRISTHVALTARALGADKIIFTTEDEHVENSVKKVVESWGGNFEFVVEKHWRKYIREFKKRGIVVHLTMYGANINEIMPEIREISRDKDILVIVGAEKVPKEVYELADYNVSVGNQPHSEVAALAIFLDRLFEGKTLYRDFEDAKIKIVPSKDGKVVIREKQNK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58805</accession>
    <name>Y1410_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0113 protein MJ1410</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1410</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0113 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64476">
        <property type="entry name" value="A64476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870927.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58805"/>
    <dbReference type="SMR" id="Q58805"/>
    <dbReference type="STRING" id="243232.MJ_1410"/>
    <dbReference type="EnsemblBacteria" id="AAB99417">
        <property type="protein sequence ID" value="AAB99417"/>
        <property type="gene ID" value="MJ_1410"/>
    </dbReference>
    <dbReference type="GeneID" id="1452313"/>
    <dbReference type="KEGG" id="mja:MJ_1410"/>
    <dbReference type="eggNOG" id="arCOG00993">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_114385_0_0_2"/>
    <dbReference type="InParanoid" id="Q58805"/>
    <dbReference type="OMA" id="AVFNKNR"/>
    <dbReference type="OrthoDB" id="127282at2157"/>
    <dbReference type="PhylomeDB" id="Q58805"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="UPF0113_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="UPF0113_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000159748" description="UPF0113 protein MJ1410">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="159" mass="18506" checksum="BFA9572469EFAAC1" modified="1996-11-01" version="1">MKVRKLKNAEINLIEEELSKYTDKDFVKSFKYENLIVLEGKWLTVCYTNIETIKKLNMFQDIFSVGNVFGEIKRKFRLSLEGFTLISPNIINNYAIVNEKAEALFLYGRDIFKESIIEVKGFGRIAVFNKNREFLGIGLFDGKIIKNIKDKGWYLREGG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58818</accession>
    <name>CFBD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ni-sirohydrochlorin a,c-diamide reductive cyclase complex, component CfbD</fullName>
            <ecNumber evidence="1">6.3.3.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">NifD homolog component CfbD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cfbD</name>
        <name type="ordered locus">MJ1423</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes both the six-electron reduction of the tetrahydroporphyrin ring system and the gamma-lactamization of the c-acetamide side chain of Ni-sirohydrochlorin a,c-diamide to yield 15,17(3)-seco-F430-17(3)-acid (seco-F430), the last intermediate in the biosynthesis of the coenzyme F430.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3 AH2 + ATP + H2O + Ni-sirohydrochlorin a,c-diamide = 15,17(3)-seco-F430-17(3)-acid + 3 A + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:52900"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:136887"/>
            <dbReference type="ChEBI" id="CHEBI:136888"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer or monomer. The Ni-sirohydrochlorin a,c-diamide reductive cyclase complex is composed of a NifH homolog component CfbC and a NifD homolog component CfbD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NifD/NifK/NifE/NifN family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.7" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64477">
        <property type="entry name" value="F64477"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870941.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58818"/>
    <dbReference type="SMR" id="Q58818"/>
    <dbReference type="STRING" id="243232.MJ_1423"/>
    <dbReference type="DNASU" id="1452327"/>
    <dbReference type="EnsemblBacteria" id="AAB99434">
        <property type="protein sequence ID" value="AAB99434"/>
        <property type="gene ID" value="MJ_1423"/>
    </dbReference>
    <dbReference type="GeneID" id="1452327"/>
    <dbReference type="KEGG" id="mja:MJ_1423"/>
    <dbReference type="eggNOG" id="arCOG04888">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_782144_0_0_2"/>
    <dbReference type="InParanoid" id="Q58818"/>
    <dbReference type="OMA" id="CASMIIG"/>
    <dbReference type="OrthoDB" id="56870at2157"/>
    <dbReference type="PhylomeDB" id="Q58818"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017675">
        <property type="entry name" value="CfbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03282">
        <property type="entry name" value="methan_mark_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0484">Methanogenesis</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107317" description="Ni-sirohydrochlorin a,c-diamide reductive cyclase complex, component CfbD">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q46FL2"/>
        </source>
    </evidence>
    <sequence length="352" mass="39470" checksum="F95B6F9C6095B0EB" modified="1996-11-01" version="1">MIFHPRPSPIAAAMYQLRDLGVDAIILHGPSGCCFRTARLLELDGVRVFTSNIDENAIVFGASENLKKALDYAIEYLKKELKKERPMIGIVGTCASMIIGEDLWEFVDDDRAIIIPVEVHSGSGDNTIGAIKAMESALKLGIIDEKEFERQKFLLKKATEVEKKRGMAKKEYIKPTYDDDLNEAIKVLKDLKEKDGKIACVLNAKKETAYLFAHPLIVLNKYFNCVNIANLDINKGLPKIRRDAQNILRRFKADYITGGLDEYPITGERAVEILKDLDVDAIVVSGVPHALPIEEIDKDIIKIGISDGPRTYHPIKEIYDYAIVELDAHAKVLGKRDIVKSRFGEILDYALE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58823</accession>
    <name>Y1428_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TPR repeat-containing protein MJ1428</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1428</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64478">
        <property type="entry name" value="C64478"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870946.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58823"/>
    <dbReference type="SMR" id="Q58823"/>
    <dbReference type="STRING" id="243232.MJ_1428"/>
    <dbReference type="EnsemblBacteria" id="AAB99438">
        <property type="protein sequence ID" value="AAB99438"/>
        <property type="gene ID" value="MJ_1428"/>
    </dbReference>
    <dbReference type="GeneID" id="1452332"/>
    <dbReference type="KEGG" id="mja:MJ_1428"/>
    <dbReference type="eggNOG" id="arCOG03038">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_481144_0_0_2"/>
    <dbReference type="InParanoid" id="Q58823"/>
    <dbReference type="OMA" id="MFNDEND"/>
    <dbReference type="OrthoDB" id="45608at2157"/>
    <dbReference type="PhylomeDB" id="Q58823"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="SSF48452"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0802">TPR repeat</keyword>
    <feature type="chain" id="PRO_0000106461" description="TPR repeat-containing protein MJ1428">
        <location>
            <begin position="1"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 1">
        <location>
            <begin position="14"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 2">
        <location>
            <begin position="48"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 3">
        <location>
            <begin position="83"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 4">
        <location>
            <begin position="116"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 5">
        <location>
            <begin position="150"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 6">
        <location>
            <begin position="199"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 7">
        <location>
            <begin position="236"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 8">
        <location>
            <begin position="269"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 9">
        <location>
            <begin position="303"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 10">
        <location>
            <begin position="344"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 11">
        <location>
            <begin position="380"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 12">
        <location>
            <begin position="414"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 13">
        <location>
            <begin position="505"/>
            <end position="538"/>
        </location>
    </feature>
    <sequence length="567" mass="66239" checksum="7CE4AC7210B7927E" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q58846</accession>
    <name>Y1451_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1451</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1451</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH1153.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64481">
        <property type="entry name" value="B64481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870971.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58846"/>
    <dbReference type="STRING" id="243232.MJ_1451"/>
    <dbReference type="EnsemblBacteria" id="AAB99462">
        <property type="protein sequence ID" value="AAB99462"/>
        <property type="gene ID" value="MJ_1451"/>
    </dbReference>
    <dbReference type="GeneID" id="1452355"/>
    <dbReference type="KEGG" id="mja:MJ_1451"/>
    <dbReference type="eggNOG" id="arCOG04866">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_541447_0_0_2"/>
    <dbReference type="InParanoid" id="Q58846"/>
    <dbReference type="OMA" id="VMARCMN"/>
    <dbReference type="OrthoDB" id="49937at2157"/>
    <dbReference type="PhylomeDB" id="Q58846"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008303">
        <property type="entry name" value="Methan_mark_14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09887">
        <property type="entry name" value="DUF2114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016937">
        <property type="entry name" value="UCP016937"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03285">
        <property type="entry name" value="methan_mark_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107338" description="Uncharacterized protein MJ1451">
        <location>
            <begin position="1"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="484" mass="52432" checksum="BA63804FEB0A0CB5" modified="1996-11-01" version="1">MGIFDVISGLFKKKPKIAYAKSQSVDLIELKRNPYYIVASVELGNTTTKSIITATNMDTGKTYIVSKHVKMTRDVRKPKKGEEVFGETLWGVELTREAVADMVKEVLLESLKKAGLTVDDLHFVVRSTGVTAGFASPEEVGEMIIALAQGCMKAGVPPAKMTPAMTKEQIPKPFDKYSFLDKIIFDGAVTGVLPPTGKEVVANEMEGELVTAGIKVGSKWTDVDFRNPCMSIDFGTTLAGRITNDTLPYAKVIGNLCGLAGAIADAIARGSGKIDEKTGAALDLANIKGKPNEELAKEYAEEMHKYIIIKEVPKDVDRFGTVPVDPKSAEKAGTTLIGCDVGKNGSDLIKLEELGRELVEKSDIPTLMCCLDYVMSEVVRRLVELAYKKGLISEKSAVGITGRAGITGRKPELIIEKLKTLEIWDKVEENVVFVEDGLALGASVMARCMNCLGTPQVPIGGVRGGGCILGLRRKWQKERGMIRD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58848</accession>
    <name>Y1453_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1453</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1453</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">To M.thermoautotrophicum MTH863.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64481">
        <property type="entry name" value="D64481"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870973.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58848"/>
    <dbReference type="SMR" id="Q58848"/>
    <dbReference type="STRING" id="243232.MJ_1453"/>
    <dbReference type="EnsemblBacteria" id="AAB99468">
        <property type="protein sequence ID" value="AAB99468"/>
        <property type="gene ID" value="MJ_1453"/>
    </dbReference>
    <dbReference type="GeneID" id="1452357"/>
    <dbReference type="KEGG" id="mja:MJ_1453"/>
    <dbReference type="eggNOG" id="arCOG04458">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_100102_0_0_2"/>
    <dbReference type="InParanoid" id="Q58848"/>
    <dbReference type="OMA" id="NTCSPYL"/>
    <dbReference type="OrthoDB" id="85931at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016733">
        <property type="entry name" value="UCP018747"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018747">
        <property type="entry name" value="UCP018747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107342" description="Uncharacterized protein MJ1453">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="192" mass="22651" checksum="7026CC8CE6A24643" modified="1996-11-01" version="1">MISEVVVTTRKNNRDNKAPIGVYFKDKKVIMHLFSGSHTYENLLTEDYFSVNVVPPIEIAKAVLDDEDDYLYYNDIPYLKSSYYAIFYKVIERKFVDREDKFGKNRLMILEGEEIKRIYLNNIPKPYNRADGLLVEIAVIYSRLANKNIKINEDDKKEMINDIRKYFSIIKKVGGREHKQLAEIMLRNLNLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q58876</accession>
    <name>Y1481_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1481</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1481</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64484">
        <property type="entry name" value="H64484"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871003.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3WKR">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.80 A"/>
        <property type="chains" value="C/D/G/H=1-213"/>
    </dbReference>
    <dbReference type="PDB" id="3WKS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.03 A"/>
        <property type="chains" value="C/D=1-103"/>
    </dbReference>
    <dbReference type="PDB" id="5X6B">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.60 A"/>
        <property type="chains" value="E/F=1-213"/>
    </dbReference>
    <dbReference type="PDB" id="5X6C">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="3.10 A"/>
        <property type="chains" value="E/F=1-213"/>
    </dbReference>
    <dbReference type="PDBsum" id="3WKR"/>
    <dbReference type="PDBsum" id="3WKS"/>
    <dbReference type="PDBsum" id="5X6B"/>
    <dbReference type="PDBsum" id="5X6C"/>
    <dbReference type="AlphaFoldDB" id="Q58876"/>
    <dbReference type="SMR" id="Q58876"/>
    <dbReference type="STRING" id="243232.MJ_1481"/>
    <dbReference type="EnsemblBacteria" id="AAB99493">
        <property type="protein sequence ID" value="AAB99493"/>
        <property type="gene ID" value="MJ_1481"/>
    </dbReference>
    <dbReference type="GeneID" id="1452387"/>
    <dbReference type="KEGG" id="mja:MJ_1481"/>
    <dbReference type="eggNOG" id="arCOG04872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108341_0_0_2"/>
    <dbReference type="OMA" id="CTEGMLD"/>
    <dbReference type="OrthoDB" id="68097at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="DisProt" id="DP00904"/>
    <dbReference type="InterPro" id="IPR016736">
        <property type="entry name" value="MJ1481_archaea"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09873">
        <property type="entry name" value="DUF2100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018814">
        <property type="entry name" value="UCP018814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107367" description="Uncharacterized protein MJ1481">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="36"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="41"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="66"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="109"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="129"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="140"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="turn" evidence="1">
        <location>
            <begin position="153"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="181"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="188"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="5X6B"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="5X6C"/>
        </source>
    </evidence>
    <sequence length="213" mass="24903" checksum="E51EDB3F72ECF733" modified="1996-11-01" version="1">MRVEYSKDLIRKGISTISQLKKAKIRVEKDDKKISYKDAKPGKIDVNEFKKAIYLLIEADDFLYKKAPKHELNEEEAKEFCKLIIKCQEHLNKILANFGFEFEEKEIDEGALYIVSNKKLFKKLKNKNPNLKVVCTEGMLDIEDMRAIGVPEKALEGLKKKVEIARKNVERFIEKYKPEKIFVVVEDDKDELLYLRAKNLYNAEKLDADEILD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q58884</accession>
    <name>Y1489_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized MCM-type protein MJ1489</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1489</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64485">
        <property type="entry name" value="H64485"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58884"/>
    <dbReference type="SMR" id="Q58884"/>
    <dbReference type="STRING" id="243232.MJ_1489"/>
    <dbReference type="EnsemblBacteria" id="AAB99502">
        <property type="protein sequence ID" value="AAB99502"/>
        <property type="gene ID" value="MJ_1489"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1489"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_2_2"/>
    <dbReference type="InParanoid" id="Q58884"/>
    <dbReference type="OMA" id="NAYTCDR"/>
    <dbReference type="PhylomeDB" id="Q58884"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007049">
        <property type="term" value="P:cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="PTHR11630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="SSF50249"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0131">Cell cycle</keyword>
    <keyword id="KW-0235">DNA replication</keyword>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000194131" description="Uncharacterized MCM-type protein MJ1489">
        <location>
            <begin position="1"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="MCM">
        <location>
            <begin position="284"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="329"/>
            <end position="336"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="682" mass="77706" checksum="9D332196B6C54049" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q58887</accession>
    <name>Y1492_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1492</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1492</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64486">
        <property type="entry name" value="C64486"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871015.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58887"/>
    <dbReference type="STRING" id="243232.MJ_1492"/>
    <dbReference type="EnsemblBacteria" id="AAB99504">
        <property type="protein sequence ID" value="AAB99504"/>
        <property type="gene ID" value="MJ_1492"/>
    </dbReference>
    <dbReference type="GeneID" id="1452399"/>
    <dbReference type="KEGG" id="mja:MJ_1492"/>
    <dbReference type="eggNOG" id="arCOG03949">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104519_0_0_2"/>
    <dbReference type="OMA" id="IFGHKMA"/>
    <dbReference type="OrthoDB" id="93562at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019206">
        <property type="entry name" value="DUF2085_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09858">
        <property type="entry name" value="DUF2085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107375" description="Uncharacterized protein MJ1492">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="156" mass="18363" checksum="CBDDBFC6F75A9CF2" modified="1996-11-01" version="1">MKKYYLIVLISFLIFYLSIFLAPYFAYLGETSNFWKFISICLYAVYSLICHQMPQRSFFIFGHKMAVCARCFGIYTGVLVGMIIYPFIKKLDDFKIPNKWYLIIALIPMAVDGTTQLIGLRESFNELRFITGFIAGFTVVFYILPIFFEMIYKKFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="122" xmlns="http://uniprot.org/uniprot">
    <accession>Q58896</accession>
    <name>FNO_METJA</name>
    <protein>
        <recommendedName>
            <fullName>F420-dependent NADP reductase</fullName>
            <ecNumber>1.5.1.40</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>F420H2:NADP oxidoreductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">fno</name>
        <name type="ordered locus">MJ1501</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reduction of NADP(+) with F420H(2) via hydride transfer, and the reverse reaction, i.e. the reduction of F420 with NADPH. Probably functions in the regeneration of NADPH required in biosynthetic reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NADP(+) + reduced coenzyme F420-(gamma-L-Glu)(n) = 2 H(+) + NADPH + oxidized coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:31363"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.1.40"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the F420-dependent NADP reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.40"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64487">
        <property type="entry name" value="D64487"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871024.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58896"/>
    <dbReference type="SMR" id="Q58896"/>
    <dbReference type="STRING" id="243232.MJ_1501"/>
    <dbReference type="EnsemblBacteria" id="AAB99514">
        <property type="protein sequence ID" value="AAB99514"/>
        <property type="gene ID" value="MJ_1501"/>
    </dbReference>
    <dbReference type="GeneID" id="1452408"/>
    <dbReference type="KEGG" id="mja:MJ_1501"/>
    <dbReference type="eggNOG" id="arCOG00457">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_076368_1_0_2"/>
    <dbReference type="InParanoid" id="Q58896"/>
    <dbReference type="OMA" id="IDTDVMV"/>
    <dbReference type="OrthoDB" id="83005at2157"/>
    <dbReference type="PhylomeDB" id="Q58896"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102261">
        <property type="term" value="F:8-hydroxy-5-deazaflavin:NADPH oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052808">
        <property type="term" value="F:reduced coenzyme F420:NADP+ oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010185">
        <property type="entry name" value="NpdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01915">
        <property type="entry name" value="npdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000087152" description="F420-dependent NADP reductase">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="9"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="30"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="D9PVP5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="O29370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="223" mass="24068" checksum="2370BBD6F5BBD3D9" modified="1996-11-01" version="1">MKIAILGGTGDQGFGLALRLAKNNKIIIGSRKKEKAEEAAKKAKEILKQRGIEADIIGLENKDAAKEGDVVILSLPYEYTLSTIKQLKEELKGKIVVSIGVPLATAIGDKPTRLLFPPDGSVAEMVQNVLKESKVVSAFQNVCHAVLEDLDNPVDCDILVCGNDEEAKKVVIDLANQIDGVRAIDCGNLEKSRIIEAITPLLIGLNIKYKSKGTGIRITNLEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q58900</accession>
    <name>Y1505_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ATP-dependent RNA helicase MJ1505</fullName>
            <ecNumber>3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1505</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O = ADP + H(+) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871028.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58900"/>
    <dbReference type="SMR" id="Q58900"/>
    <dbReference type="STRING" id="243232.MJ_1505"/>
    <dbReference type="EnsemblBacteria" id="AAB99518">
        <property type="protein sequence ID" value="AAB99518"/>
        <property type="gene ID" value="MJ_1505"/>
    </dbReference>
    <dbReference type="GeneID" id="1452412"/>
    <dbReference type="KEGG" id="mja:MJ_1505"/>
    <dbReference type="eggNOG" id="arCOG00872">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002513_3_1_2"/>
    <dbReference type="InParanoid" id="Q58900"/>
    <dbReference type="OMA" id="HRAVGNY"/>
    <dbReference type="OrthoDB" id="3323at2157"/>
    <dbReference type="PhylomeDB" id="Q58900"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12089">
        <property type="entry name" value="Hef_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041663">
        <property type="entry name" value="DisA/LigA_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039686">
        <property type="entry name" value="FANCM/Mph1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041755">
        <property type="entry name" value="Hef_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="PTHR14025:SF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12826">
        <property type="entry name" value="HHH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="SSF47781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="SSF52980"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0347">Helicase</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <feature type="chain" id="PRO_0000055117" description="Putative ATP-dependent RNA helicase MJ1505">
        <location>
            <begin position="1"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="338"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="DEAH box">
        <location>
            <begin position="137"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="778" mass="88883" checksum="DCB1F220801338D8" modified="1998-06-01" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="139" xmlns="http://uniprot.org/uniprot">
    <accession>Q58923</accession>
    <name>TPIS_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 3">Triosephosphate isomerase</fullName>
            <shortName evidence="1 3">TIM</shortName>
            <shortName evidence="1">TPI</shortName>
            <ecNumber evidence="1 2">5.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Triose-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">tpiA</name>
        <name type="ordered locus">MJ1528</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="Acta Crystallogr. D" volume="63" first="206" last="220">
            <title>Structure of triosephosphate isomerase (TIM) from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Gayathri P."/>
                <person name="Banerjee M."/>
                <person name="Vijayalakshmi A."/>
                <person name="Azeez S."/>
                <person name="Balaram H."/>
                <person name="Balaram P."/>
                <person name="Murthy M.R."/>
            </authorList>
            <dbReference type="PubMed" id="17242514"/>
            <dbReference type="DOI" id="10.1107/S0907444906046488"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 3-219</scope>
        <scope>FUNCTION AS A TRIOSEPHOSPHATE ISOMERASE</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1 2">Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="biophysicochemical properties">
        <temperatureDependence>
            <text evidence="2">The enzyme is active over the temperature range 20-60 degrees Celsius, showing an increase in specific activity with temperature.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="1 2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64490">
        <property type="entry name" value="G64490"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871052.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2H6R">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D/E/F/G/H=1-219"/>
    </dbReference>
    <dbReference type="PDBsum" id="2H6R"/>
    <dbReference type="AlphaFoldDB" id="Q58923"/>
    <dbReference type="SMR" id="Q58923"/>
    <dbReference type="STRING" id="243232.MJ_1528"/>
    <dbReference type="EnsemblBacteria" id="AAB99546">
        <property type="protein sequence ID" value="AAB99546"/>
        <property type="gene ID" value="MJ_1528"/>
    </dbReference>
    <dbReference type="GeneID" id="1452436"/>
    <dbReference type="KEGG" id="mja:MJ_1528"/>
    <dbReference type="eggNOG" id="arCOG01087">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_104921_0_0_2"/>
    <dbReference type="InParanoid" id="Q58923"/>
    <dbReference type="OMA" id="IPVYAQH"/>
    <dbReference type="OrthoDB" id="60670at2157"/>
    <dbReference type="PhylomeDB" id="Q58923"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-14589"/>
    <dbReference type="BRENDA" id="5.3.1.1">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="EvolutionaryTrace" id="Q58923"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0312">Gluconeogenesis</keyword>
    <keyword id="KW-0324">Glycolysis</keyword>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000090335" description="Triosephosphate isomerase">
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    <sequence length="219" mass="23319" checksum="C6BC49A2DEE8A026" modified="1997-11-01" version="1">MLIVINYKTYNESIGNRGLEIAKIAEKVSEESGITIGVAPQFVDLRMIVENVNIPVYAQHIDNINPGSHTGHILAEAIKDCGCKGTLINHSEKRMLLADIEAVINKCKNLGLETIVCTNNINTSKAVAALSPDYIAVEPPELIGTGIPVSKANPEVVEGTVRAVKEINKDVKVLCGAGISKGEDVKAALDLGAEGVLLASGVVKAKNVEEAIRELIKFI</sequence>
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<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q58936</accession>
    <name>DADD_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 4">5'-deoxyadenosine deaminase</fullName>
            <shortName evidence="1 4">5'-dA deaminase</shortName>
            <ecNumber evidence="1 2">3.5.4.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">5'-methylthioadenosine deaminase</fullName>
            <shortName evidence="5">MTA deaminase</shortName>
            <ecNumber evidence="2">3.5.4.31</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Adenosine deaminase</fullName>
            <ecNumber evidence="2">3.5.4.4</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5 6">S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="5 6">SAH deaminase</shortName>
            <ecNumber evidence="2 3">3.5.4.28</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1 4" type="primary">dadD</name>
        <name type="ordered locus">MJ1541</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2014" name="J. Bacteriol." volume="196" first="1064" last="1072">
            <title>Identification of a 5'-deoxyadenosine deaminase in Methanocaldococcus jannaschii and its possible role in recycling the radical S-adenosylmethionine enzyme reaction product 5'-deoxyadenosine.</title>
            <authorList>
                <person name="Miller D."/>
                <person name="O'Brien K."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="24375099"/>
            <dbReference type="DOI" id="10.1128/JB.01308-13"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF TYR-136; GLU-150 AND CYS-294</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2015" name="J. Bacteriol." volume="197" first="2284" last="2291">
            <title>S-Inosyl-L-Homocysteine Hydrolase, a Novel Enzyme Involved in S-Adenosyl-L-Methionine Recycling.</title>
            <authorList>
                <person name="Miller D."/>
                <person name="Xu H."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="25917907"/>
            <dbReference type="DOI" id="10.1128/JB.00080-15"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>PATHWAY</scope>
    </reference>
    <comment type="function">
        <text evidence="2 6">Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'-methylthioadenosine, to produce the inosine analogs. Can also deaminate adenosine. The preferred substrate for this enzyme is 5'-deoxyadenosine, but all these substrates are efficiently deaminated (PubMed:24375099). Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions (PubMed:25917907). May also be involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens (PubMed:24375099).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 2">
            <text>5'-deoxyadenosine + H(+) + H2O = 5'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:42892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:82775"/>
            <dbReference type="EC" id="3.5.4.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:42893"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H(+) + H2O + S-adenosyl-L-homocysteine = NH4(+) + S-inosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5 6">
            <dbReference type="Rhea" id="RHEA:20717"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>H(+) + H2O + S-methyl-5'-thioadenosine = NH4(+) + S-methyl-5'-thioinosine</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:25026"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>adenosine + H(+) + H2O = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:24409"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.014 mM for 5'-deoxyadenosine (at pH 9 and 60 degrees Celsius)</KM>
            <KM evidence="2">0.11 mM for S-methyl-5'-thioadenosine (at pH 9 and 60 degrees Celsius)</KM>
            <KM evidence="2">1.1 mM for S-adenosyl-L-homocysteine (at pH 9 and 60 degrees Celsius)</KM>
            <KM evidence="2">0.15 mM for adenosine (at pH 9 and 60 degrees Celsius)</KM>
            <text evidence="2">kcat is 120000 sec(-1) for 5'-deoxyadenosine deamination. kcat is 160 sec(-1) for S-methyl-5'-thioadenosine deamination. kcat is 1300 sec(-1) for S-adenosyl-L-homocysteine deamination. kcat is 110 sec(-1) for adenosine deamination (at pH 9 and 60 degrees Celsius).</text>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 9.0.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5 6">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine (PubMed:25917907). 5'-deoxyadenosine is a radical SAM enzyme reaction product which strongly inhibits radical SAM enzymes. A pathway for removing this product must be present in methanogens where the MTA/SAH nucleosidase which normally metabolizes this compound is absent (PubMed:24375099).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="NC_000909.1"/>
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    <dbReference type="HOGENOM" id="CLU_012358_2_1_2"/>
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        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01281">
        <property type="entry name" value="MTA_SAH_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023512">
        <property type="entry name" value="Deaminase_MtaD/DadD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="SSF51338"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="SSF51556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000122311" description="5'-deoxyadenosine deaminase">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Lowers temperature stability by 10 degrees Celsius and decreases activity by 364-fold with 5'-deoxyadenosine as substrate. Lowers temperature stability by 35 degrees Celsius and decreases activity by 100000-fold with 5'-deoxyadenosine as substrate; when associated with R-150." evidence="2">
        <original>Y</original>
        <variation>R</variation>
        <location>
            <position position="136"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Lowers temperature stability by 10 degrees Celsius and decreases activity by 571-fold with 5'-deoxyadenosine as substrate. Lowers temperature stability by 35 degrees Celsius and decreases activity by 100000-fold with 5'-deoxyadenosine as substrate; when associated with R-136." evidence="2">
        <original>E</original>
        <variation>R</variation>
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No effect on temperature stability." evidence="2">
        <original>C</original>
        <variation>S</variation>
        <location>
            <position position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="24375099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="25917907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="4">
        <source>
            <dbReference type="PubMed" id="24375099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="24375099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="6">
        <source>
            <dbReference type="PubMed" id="25917907"/>
        </source>
    </evidence>
    <sequence length="420" mass="47468" checksum="2458E0E14AF91731" modified="1996-11-01" version="1">MILIKNVFVNGKRQDILIEGNKIKKIGEVKKEEIENAEIIDGKNKIAIPGLINTHTHIPMTLFRGVADDLPLMEWLNNYIWPMEAKLNEEIVYWGTLLGCIEMIRSGTTTFNDMYFFLEGIAKAVDESGMRAVLAYGMIDLFDEERRERELKNAEKYINYINSLNNSRIMPALGPHAPYTCSKELLMEVNNLAKKYNVPIHIHLNETLDEIKMVKEKTGMEPFIYLNSFGFFDDVRAIAAHCVHLTDEEIKIMKQKNINVSHNPISNLKLASGVAPIPKLLAEGINVTLGTDGCGSNNNLNLFEEIKVSAILHKGVNLNPTVVKAEEAFNFATKNGAKALNIKAGEIREGYLADIVLINLDKPYLYPKENIMSHLVYAFNGFVDDVIIDGNIVMRDGEILTVDEEKVYEKAEEMYEILRS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-04-23" modified="2022-05-25" version="96" xmlns="http://uniprot.org/uniprot">
    <accession>Q58953</accession>
    <name>Y1558_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0237 protein MJ1558</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1558</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0237 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64494">
        <property type="entry name" value="E64494"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871082.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58953"/>
    <dbReference type="SMR" id="Q58953"/>
    <dbReference type="STRING" id="243232.MJ_1558"/>
    <dbReference type="EnsemblBacteria" id="AAB99581">
        <property type="protein sequence ID" value="AAB99581"/>
        <property type="gene ID" value="MJ_1558"/>
    </dbReference>
    <dbReference type="GeneID" id="1452466"/>
    <dbReference type="KEGG" id="mja:MJ_1558"/>
    <dbReference type="eggNOG" id="arCOG04941">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155669_0_1_2"/>
    <dbReference type="InParanoid" id="Q58953"/>
    <dbReference type="OMA" id="MIMLVDI"/>
    <dbReference type="OrthoDB" id="116913at2157"/>
    <dbReference type="PhylomeDB" id="Q58953"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04872">
        <property type="entry name" value="ACT_1ZPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01054">
        <property type="entry name" value="UPF0237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022986">
        <property type="entry name" value="UPF0237_ACT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000219910" description="UPF0237 protein MJ1558">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="5"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01054"/>
        </source>
    </evidence>
    <sequence length="90" mass="9988" checksum="A477B9511CBED489" modified="1998-06-01" version="2">MSRVVVSVIGQDRTGIVAGISKVLAENNANILDISQTIMDNLFAMIMLVDISNAKVDFATLKKELEKAGEELGVQVIVQHEDIFKYMHRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="66" xmlns="http://uniprot.org/uniprot">
    <accession>Q58968</accession>
    <name>Y1573_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1573</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1573</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64496">
        <property type="entry name" value="D64496"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58968"/>
    <dbReference type="STRING" id="243232.MJ_1573"/>
    <dbReference type="EnsemblBacteria" id="AAB99595">
        <property type="protein sequence ID" value="AAB99595"/>
        <property type="gene ID" value="MJ_1573"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1573"/>
    <dbReference type="HOGENOM" id="CLU_2911527_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107421" description="Uncharacterized protein MJ1573">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <sequence length="61" mass="7298" checksum="11F1BD4328B6BF5A" modified="1996-11-01" version="1">MVMLFPDYIRDVNQMKSSLSFLDKIEEDDLAISIYRDRKLSRKELFPIYLGATTHLHKECY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-03-01" modified="2022-05-25" version="125" xmlns="http://uniprot.org/uniprot">
    <accession>Q58970</accession>
    <name>GUAAA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="1">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">guaAA</name>
        <name type="ordered locus">MJ1575</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99597.1"/>
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        <property type="method" value="NMR"/>
        <property type="chains" value="A=1-188"/>
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        <property type="term" value="P:glutamine metabolic process"/>
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        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
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        <property type="entry name" value="GATASE"/>
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        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="guaA_Nterm"/>
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    </dbReference>
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    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0332">GMP biosynthesis</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0658">Purine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140221" description="GMP synthase [glutamine-hydrolyzing] subunit A">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-1" evidence="1">
        <location>
            <begin position="1"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="1">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="163"/>
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    </feature>
    <feature type="active site" evidence="1">
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    <feature type="strand" evidence="3">
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            <begin position="2"/>
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        </location>
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    <feature type="strand" evidence="3">
        <location>
            <begin position="26"/>
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            <begin position="31"/>
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    <feature type="strand" evidence="3">
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    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="60"/>
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        </location>
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    <feature type="strand" evidence="3">
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    </feature>
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        </location>
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    <feature type="strand" evidence="3">
        <location>
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    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="110"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="117"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="126"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="136"/>
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        </location>
    </feature>
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        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="strand" evidence="3">
        <location>
            <begin position="148"/>
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        </location>
    </feature>
    <feature type="strand" evidence="2">
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            <begin position="166"/>
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        </location>
    </feature>
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        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="2LXN"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="7D97"/>
        </source>
    </evidence>
    <sequence length="188" mass="21020" checksum="3F1DDB0FA1F2D850" modified="1996-11-01" version="1">MIVILDNGGQYVHRIHRSLKYIGVSSKIVPNTTPLEEIESNKEVKGIILSGGPDIEKAKNCIDIALNAKLPILGICLGHQLIALAYGGEVGRAEAEEYALTKVYVDKENDLFKNVPREFNAWASHKDEVKKVPEGFEILAHSDICQVEAMKHKTKPIYGVQFHPEVAHTEYGNEILKNFCKVCGYKFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-14" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q59002</accession>
    <name>Y1607_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized glycosyltransferase MJ1607</fullName>
            <ecNumber>2.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1607</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.-.-"/>
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    </dbReference>
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    <dbReference type="STRING" id="243232.MJ_1607"/>
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        <property type="family name" value="Glycosyltransferase Family 4"/>
    </dbReference>
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        <property type="protein sequence ID" value="AAB99629"/>
        <property type="gene ID" value="MJ_1607"/>
    </dbReference>
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        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_4-like_N"/>
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        <property type="entry name" value="Glyco_transf_4"/>
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    </dbReference>
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        <property type="entry name" value="Glycos_transf_1"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000080324" description="Uncharacterized glycosyltransferase MJ1607">
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            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="390" mass="44446" checksum="17C12B7BF7B06556" modified="1996-11-01" version="1">MKIAMVTWEYPPRIVGGLAIHCKGLAEGLVRNGHEVDVITVGYDLPEYENINGVNVYRVRPISHPHFLTWAMFMAEEMEKKLGILGVDKYDVIHCHDWMTHFVGANLKHICRMPYVQSIHSTEIGRCGGLYSDDSKAIHAMEYLSTYESCQVITVSKSLKEEVCSIFNTPEDKVKVIYNGINPWEFDINLSWEEKINFRRSIGVQDDEKMILFVGRLTYQKGIEYLIRAMPKILERHNAKLVIAGSGDMRDYLEDLCYQLGVRHKVVFLGFVNGDTLKKLYKSADVVVIPSVYEPFGIVALEAMAAGTPVVVSSVGGLMEIIKHEVNGIWVYPKNPDSIAWGVDRVLSDWGFREYIVNNAKKDVYEKYSWDNIAKETVNVYKIAMEMMGR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q59065</accession>
    <name>Y1671_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1671</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1671</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64508">
        <property type="entry name" value="E64508"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59065"/>
    <dbReference type="SMR" id="Q59065"/>
    <dbReference type="STRING" id="243232.MJ_1671"/>
    <dbReference type="EnsemblBacteria" id="AAB99694">
        <property type="protein sequence ID" value="AAB99694"/>
        <property type="gene ID" value="MJ_1671"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1671"/>
    <dbReference type="HOGENOM" id="CLU_3194507_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107469" description="Uncharacterized protein MJ1671">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <sequence length="45" mass="5353" checksum="0AFB6B337C51D337" modified="1996-11-01" version="1">MEYVKFLEDYVREKVKKMFSSNVKLSFNDLKVSAKIVELKKRGVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-05-25" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q59074</accession>
    <name>Y1680_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1680</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1680</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64509">
        <property type="entry name" value="F64509"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59074"/>
    <dbReference type="STRING" id="243232.MJ_1680"/>
    <dbReference type="EnsemblBacteria" id="AAB99701">
        <property type="protein sequence ID" value="AAB99701"/>
        <property type="gene ID" value="MJ_1680"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1680"/>
    <dbReference type="eggNOG" id="arCOG02120">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_116617_1_0_2"/>
    <dbReference type="InParanoid" id="Q59074"/>
    <dbReference type="OMA" id="DDNKWLE"/>
    <dbReference type="PhylomeDB" id="Q59074"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002850">
        <property type="entry name" value="PIN_toxin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34610">
        <property type="entry name" value="PTHR34610"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13470">
        <property type="entry name" value="PIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="SSF88723"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00305">
        <property type="entry name" value="TIGR00305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107480" description="Uncharacterized protein MJ1680">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <sequence length="155" mass="17778" checksum="57D1306D3F7948C7" modified="1996-11-01" version="1">MEKMEKIKIKVVIDTNVFISALINPNGIPGKILDLIFEEKIVNYTSPSILKEIGFKCLSPKLRKYLGNENRILKILTAFSSVSVIINPNTNFNVCRDEDDNKFINVAYESKAIIITGDKDLISLRDENKYLKINNIHLKVPTPKEFIEEFEKFIS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>Q59075</accession>
    <name>Y1681_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1681</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1681</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64509">
        <property type="entry name" value="G64509"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871205.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59075"/>
    <dbReference type="STRING" id="243232.MJ_1681"/>
    <dbReference type="EnsemblBacteria" id="AAB99702">
        <property type="protein sequence ID" value="AAB99702"/>
        <property type="gene ID" value="MJ_1681"/>
    </dbReference>
    <dbReference type="GeneID" id="1452590"/>
    <dbReference type="KEGG" id="mja:MJ_1681"/>
    <dbReference type="eggNOG" id="arCOG00621">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061500_0_0_2"/>
    <dbReference type="InParanoid" id="Q59075"/>
    <dbReference type="OMA" id="GCGEINP"/>
    <dbReference type="OrthoDB" id="22946at2157"/>
    <dbReference type="PhylomeDB" id="Q59075"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017677">
        <property type="entry name" value="Methan_mark_16"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03287">
        <property type="entry name" value="methan_mark_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107481" description="Uncharacterized protein MJ1681">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="287"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="380" mass="42581" checksum="D362219799F4796A" modified="1996-11-01" version="1">MEKELKVITIDELKKYIRNNEEDKIEEVDVVTSATCGIMSGTAGIFHIPFNEVFKRAEEIYLNDIKGVVGICPNEFLGKVDAIFYGEVGFLFKDLVKGKVVEAKAISEGKIYKNEITIDDLPTAKMIGTRMAFKNYTAITNLSDEEVNTIFHRLPLKKGEASFSGCGMLNPLENMVIKDEKDVVGKKALLNGAEAIILGFGTRASIEKPNLMMSADMKDMDAYYLGGFVTSNGIEIYNTIAVPIKVDEHKEALKKLDKDITLPLVNIFGREIIDIGSYAEVWENVDLRPKIYQDKCKNCRECLVEKYCPTFAIKRENGKIKITEDCFGCGVCNICPYGVFKTKLGSVCGIPITCRQSDRKRALKLAKELKKKIERGEFKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="138" xmlns="http://uniprot.org/uniprot">
    <accession>Q60173</accession>
    <name>ENO_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Enolase</fullName>
            <ecNumber evidence="1">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">eno</name>
        <name type="ordered locus">MJ0232</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = H2O + phosphoenolpyruvate</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="1">The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell surface</location>
        </subcellularLocation>
        <text evidence="1">Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the enolase family.</text>
    </comment>
    <comment type="sequence caution" evidence="2">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98220" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98220.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64329">
        <property type="entry name" value="A64329"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496902.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2PA6">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.85 A"/>
        <property type="chains" value="A/B=1-423"/>
    </dbReference>
    <dbReference type="PDBsum" id="2PA6"/>
    <dbReference type="AlphaFoldDB" id="Q60173"/>
    <dbReference type="SMR" id="Q60173"/>
    <dbReference type="STRING" id="243232.MJ_0232"/>
    <dbReference type="PRIDE" id="Q60173"/>
    <dbReference type="EnsemblBacteria" id="AAB98220">
        <property type="protein sequence ID" value="AAB98220"/>
        <property type="gene ID" value="MJ_0232"/>
    </dbReference>
    <dbReference type="GeneID" id="1451085"/>
    <dbReference type="KEGG" id="mja:MJ_0232"/>
    <dbReference type="eggNOG" id="arCOG01169">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031223_2_1_2"/>
    <dbReference type="InParanoid" id="Q60173"/>
    <dbReference type="OMA" id="EFMIIPV"/>
    <dbReference type="OrthoDB" id="23221at2157"/>
    <dbReference type="PhylomeDB" id="Q60173"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q60173"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
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    <sequence length="423" mass="46298" checksum="9C9BB163E64BF577" modified="2001-02-21" version="2">MDERFEIKDIVAREVIDSRGNPTVEVEVITKGNGYGSAIVPSGASTGTHEALELRDKEKRFGGKGVLMAVENVNSIIRPEILGYDARMQREIDTIMIELDGTPNKSRLGANAILAVSLAVAKAAAATAKIPLYKYLGGFNSYVMPVPMMNVINGGKHAGNDLDLQEFMIMPVGATSISEAVRMGSEVYHVLKNVILEKYGKNAVNVGDEGGFAPPLKTSREALDLLTESVKKAGYEDEVVFALDAAASEFYKDGYYYVEGKKLTREELLDYYKALVDEYPIVSIEDPFHEEDFEGFAMITKELDIQIVGDDLFVTNVERLRKGIEMKAANALLLKVNQIGTLSEAVDAAQLAFRNGYGVVVSHRSGETEDTTIADLSVALNSGQIKTGAPARGERTAKYNQLIRIEQELGLSKYAGRNFRCPF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q60269</accession>
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            <fullName>Uncharacterized protein MJECL07</fullName>
        </recommendedName>
    </protein>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="75" xmlns="http://uniprot.org/uniprot">
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        </lineage>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_1259098_0_0_2"/>
    <dbReference type="InParanoid" id="Q60272"/>
    <dbReference type="OMA" id="VYPYYLM"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009432">
        <property type="term" value="P:SOS response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004604">
        <property type="entry name" value="DNA_recomb/repair_RecN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11059">
        <property type="entry name" value="PTHR11059"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107502" description="Uncharacterized protein MJECL10">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <sequence length="219" mass="25362" checksum="1F147DF46DAC9017" modified="1997-11-01" version="1">MHIHLFRCQCMIETIHIKNFRGIRELKLENLGQINIIAGKNNASKSSILEALALFLSAKEGFSLFIKILREILLWRGWYGEKSIYDLFYKNSKELEVSVKFLNQDFANLTLKNSNQSFANKNIAVELKSDKNSWSGRFDSHLIHPDYISSILTSAEATQSNFEFITSLTLIKFGYIESIYSQAYETQVLQDAIRLLREAYPEVKSLSPLQKYNKWIIHV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q60307</accession>
    <name>Y3408_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECS08</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECS08</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>small ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77119">
        <property type="protein sequence ID" value="AAC37066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64516">
        <property type="entry name" value="H64516"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890101.1">
        <property type="nucleotide sequence ID" value="NC_001733.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60307"/>
    <dbReference type="STRING" id="243232.MJ_ECS08"/>
    <dbReference type="EnsemblBacteria" id="AAC37066">
        <property type="protein sequence ID" value="AAC37066"/>
        <property type="gene ID" value="MJ_ECS08"/>
    </dbReference>
    <dbReference type="GeneID" id="1450835"/>
    <dbReference type="KEGG" id="mja:MJ_ECS08"/>
    <dbReference type="eggNOG" id="arCOG00439">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_288651_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid small ECE"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="SSF46785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107489" description="Uncharacterized protein MJECS08">
        <location>
            <begin position="1"/>
            <end position="1064"/>
        </location>
    </feature>
    <sequence length="1064" mass="125624" checksum="3F57ECCF0ACEA482" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q60389</accession>
    <name>Y079_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0079</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0079</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64309">
        <property type="entry name" value="G64309"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60389"/>
    <dbReference type="SMR" id="Q60389"/>
    <dbReference type="STRING" id="243232.MJ_0079"/>
    <dbReference type="EnsemblBacteria" id="AAB98059">
        <property type="protein sequence ID" value="AAB98059"/>
        <property type="gene ID" value="MJ_0079"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0079"/>
    <dbReference type="eggNOG" id="arCOG00436">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018678_0_1_2"/>
    <dbReference type="InParanoid" id="Q60389"/>
    <dbReference type="OMA" id="HIYISDR"/>
    <dbReference type="PhylomeDB" id="Q60389"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045427">
        <property type="entry name" value="MoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041538">
        <property type="entry name" value="RavA-like_AAA_lid"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17868">
        <property type="entry name" value="AAA_lid_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20030">
        <property type="entry name" value="bpMoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106685" description="Uncharacterized protein MJ0079">
        <location>
            <begin position="1"/>
            <end position="380"/>
        </location>
    </feature>
    <sequence length="380" mass="44550" checksum="E81A7A8C5365AE59" modified="1997-11-01" version="1">MSEIMHKLEKIREELNSYFLERREEIDIALTSILANEHTVFLGNPGVAKSQLIRAIASHINANYFEKLITRFTTEDELFGPLSIKELKDNDRFVRKTSGYLPTAEIAFLDEVFKANSSILNALLSIINERIYHNGDKIEKVPLISLFGASNELPEENELLAFYDRFLFRKVVRGIRSCENLVKLIKLDEEYKPKTTISIKELRKMQEKANEVDIENIIGYLVDIKKKLSQNHIYISDRRFKKSVKAIKCFAYLNGRREAEIEDLEILRHIFWDDIDDILIVSKVIFDITNKYAEQVLDKAEIIKNLKNELKYIDIKKIGECKKDYNKLIEILCKMAYIRLELKKIRNEAIINKRKTDFIDEVIKETDEFNNYIEGILNEL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q60392</accession>
    <name>Y084_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized ATP-binding protein MJ0084</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0084</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64310">
        <property type="entry name" value="D64310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869576.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60392"/>
    <dbReference type="SMR" id="Q60392"/>
    <dbReference type="STRING" id="243232.MJ_0084"/>
    <dbReference type="EnsemblBacteria" id="AAB98064">
        <property type="protein sequence ID" value="AAB98064"/>
        <property type="gene ID" value="MJ_0084"/>
    </dbReference>
    <dbReference type="GeneID" id="1450923"/>
    <dbReference type="KEGG" id="mja:MJ_0084"/>
    <dbReference type="eggNOG" id="arCOG00588">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082962_1_0_2"/>
    <dbReference type="InParanoid" id="Q60392"/>
    <dbReference type="OMA" id="MKISICG"/>
    <dbReference type="OrthoDB" id="51644at2157"/>
    <dbReference type="PhylomeDB" id="Q60392"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106686" description="Uncharacterized ATP-binding protein MJ0084">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <sequence length="249" mass="27655" checksum="C84434BC1913D023" modified="1997-11-01" version="1">MKISICGKGGCGKSSITTLLAKEFAKKGHNVLVIDGDESNLSLHKLLGMDLPKDFIEYLGGRKEFMKKLREKMDGKEVELFEGEISIDSLPKEYLVEKDNIKLLAIGKIHDFGEGCACPMGALLREFLKSLKLKDKEVVIVDTEAGIEHFGRGVEGGCDVIIAIIDPTYESIRLSKKIEEIGEKLGKKVYFIVNKVDDETKDLILENVNKDKVIAVIPNNKEIMKCGLMGEELNAELSEIKDVVEILTK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>P54018</accession>
    <name>RS19_METJA</name>
    <protein>
        <recommendedName>
            <fullName>30S ribosomal protein S19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">rps19</name>
        <name type="ordered locus">MJ0180</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64322">
        <property type="entry name" value="E64322"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869675.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54018"/>
    <dbReference type="SMR" id="P54018"/>
    <dbReference type="STRING" id="243232.MJ_0180"/>
    <dbReference type="EnsemblBacteria" id="AAB98165">
        <property type="protein sequence ID" value="AAB98165"/>
        <property type="gene ID" value="MJ_0180"/>
    </dbReference>
    <dbReference type="GeneID" id="8803928"/>
    <dbReference type="KEGG" id="mja:MJ_0180"/>
    <dbReference type="eggNOG" id="arCOG04099">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_097347_1_1_2"/>
    <dbReference type="InParanoid" id="P54018"/>
    <dbReference type="OMA" id="VIRTHCR"/>
    <dbReference type="OrthoDB" id="99236at2157"/>
    <dbReference type="PhylomeDB" id="P54018"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_S15/S19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_S19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_S19_S15_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_S19A/S15e"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="PTHR11880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="SSF54570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000130002" description="30S ribosomal protein S19">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="152" mass="17680" checksum="F098F2AEA7DE6212" modified="1996-10-01" version="1">MASARRRRIKKKKQVISKKIEFRYRGYTLEELQQMPLREFAKLLPARQRRTLLRGLTPQQKKLAMKIKKARRLLNKGKEPRIIRTHCRDFVITPDMVGLTFGVYNGKEFVEVKVTPEMIGHYLGEFSLTRKPVQHGAPGMGATRSSMFVPIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>P54022</accession>
    <name>RL18E_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18e</name>
        <name type="ordered locus">MJ0193</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869688.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54022"/>
    <dbReference type="SMR" id="P54022"/>
    <dbReference type="STRING" id="243232.MJ_0193"/>
    <dbReference type="EnsemblBacteria" id="AAB98173">
        <property type="protein sequence ID" value="AAB98173"/>
        <property type="gene ID" value="MJ_0193"/>
    </dbReference>
    <dbReference type="GeneID" id="1451041"/>
    <dbReference type="KEGG" id="mja:MJ_0193"/>
    <dbReference type="eggNOG" id="arCOG00780">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_146465_0_0_2"/>
    <dbReference type="InParanoid" id="P54022"/>
    <dbReference type="OMA" id="WRLERPR"/>
    <dbReference type="OrthoDB" id="104254at2157"/>
    <dbReference type="PhylomeDB" id="P54022"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="L18e/L15P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_L15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021132">
        <property type="entry name" value="Ribosomal_L18/L18-A/B/e_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_L18e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_L18e/L15P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_L18e_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="PTHR10934"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="SSF52080"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01106">
        <property type="entry name" value="RIBOSOMAL_L18E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000132790" description="50S ribosomal protein L18e">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <sequence length="121" mass="13532" checksum="C61544AB196CAA17" modified="1998-07-15" version="2">MAKKITATNPRLVKLIEILKQESYKNQAKIWKDIARRLAKPRRRRAEVNLSKINRYTKEGDVVLVPGKVLGAGKLEHKVVVAAFAFSETAKKLIKEAGGEAITIEELIKRNPKGSNVKIMA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="113" xmlns="http://uniprot.org/uniprot">
    <accession>P54046</accession>
    <name>RL30_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl30</name>
        <name type="ordered locus">MJ0476</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64359">
        <property type="entry name" value="D64359"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869977.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54046"/>
    <dbReference type="SMR" id="P54046"/>
    <dbReference type="STRING" id="243232.MJ_0476"/>
    <dbReference type="EnsemblBacteria" id="AAB98465">
        <property type="protein sequence ID" value="AAB98465"/>
        <property type="gene ID" value="MJ_0476"/>
    </dbReference>
    <dbReference type="GeneID" id="1451338"/>
    <dbReference type="KEGG" id="mja:MJ_0476"/>
    <dbReference type="eggNOG" id="arCOG04086">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055156_6_0_2"/>
    <dbReference type="InParanoid" id="P54046"/>
    <dbReference type="OMA" id="KDYITWG"/>
    <dbReference type="OrthoDB" id="79065at2157"/>
    <dbReference type="PhylomeDB" id="P54046"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_L30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="L30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_L30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_L30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_L30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_L7/L30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_L7_euk_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="PTHR11524"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="SSF55129"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000104624" description="50S ribosomal protein L30">
        <location>
            <begin position="1"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <sequence length="154" mass="17586" checksum="66F69859AD56798B" modified="1996-10-01" version="1">MAYAVIRIRGRVGVRRDIADTLKMLRLHKVNHCVIIPETETFKGMLQKVKDYVTWGEIDKDTLVKLILKRGRLPGNKKVNPEIIKELTGMDVEELAEKIINGEIKLKETPLKPVFRLHPPRKGFERGGIKKPFSVGGALGYRGEKINELLEKMM</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>P54052</accession>
    <name>RL18A_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">50S ribosomal protein L18Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein L20e</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">50S ribosomal protein LX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpl18a</name>
        <name evidence="1" type="synonym">rpl20e</name>
        <name evidence="1" type="synonym">rplX</name>
        <name type="ordered locus">MJ0595</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64374">
        <property type="entry name" value="C64374"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54052"/>
    <dbReference type="SMR" id="P54052"/>
    <dbReference type="STRING" id="243232.MJ_0595"/>
    <dbReference type="EnsemblBacteria" id="AAB98588">
        <property type="protein sequence ID" value="AAB98588"/>
        <property type="gene ID" value="MJ_0595"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0595"/>
    <dbReference type="eggNOG" id="arCOG04175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_177460_0_1_2"/>
    <dbReference type="InParanoid" id="P54052"/>
    <dbReference type="OMA" id="MVFTKEY"/>
    <dbReference type="PhylomeDB" id="P54052"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_L18Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="50S_L18Ae/Ribosomal_L18a/L20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_L18a//L18Ae/LX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <feature type="chain" id="PRO_0000153698" description="50S ribosomal protein L18Ae">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <sequence length="76" mass="8860" checksum="7C14762642D01E80" modified="1996-10-01" version="1">MAKIFRITGIMSKKGKDPLYFRKEYKALKPEDALEILYSEFGGRYKVKRSRIKILNIEEIKPEDVTDPVLKKLVTA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-05-25" version="105" xmlns="http://uniprot.org/uniprot">
    <accession>P54059</accession>
    <name>RS3A_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S3Ae</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Ribosomal protein S1e</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps3ae</name>
        <name type="ordered locus">MJ0980</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64422">
        <property type="entry name" value="D64422"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870494.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54059"/>
    <dbReference type="SMR" id="P54059"/>
    <dbReference type="STRING" id="243232.MJ_0980"/>
    <dbReference type="PRIDE" id="P54059"/>
    <dbReference type="EnsemblBacteria" id="AAB98983">
        <property type="protein sequence ID" value="AAB98983"/>
        <property type="gene ID" value="MJ_0980"/>
    </dbReference>
    <dbReference type="GeneID" id="1451878"/>
    <dbReference type="KEGG" id="mja:MJ_0980"/>
    <dbReference type="eggNOG" id="arCOG04186">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062507_1_0_2"/>
    <dbReference type="InParanoid" id="P54059"/>
    <dbReference type="OMA" id="GQNAYTK"/>
    <dbReference type="OrthoDB" id="96138at2157"/>
    <dbReference type="PhylomeDB" id="P54059"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00359">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030838">
        <property type="entry name" value="30S_S3Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001593">
        <property type="entry name" value="Ribosomal_S3Ae"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018281">
        <property type="entry name" value="Ribosomal_S3Ae_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01015">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01397">
        <property type="entry name" value="Ribosomal_S3Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01191">
        <property type="entry name" value="RIBOSOMAL_S3AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <feature type="chain" id="PRO_0000153548" description="30S ribosomal protein S3Ae">
        <location>
            <begin position="1"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00359"/>
        </source>
    </evidence>
    <sequence length="222" mass="25777" checksum="D3B18E711B25A41F" modified="1998-12-15" version="2">MATARTARSRRKVRKVRDKWKEKVWYEIYATPEFGGVFIGYTPANDPSLVLGRVAETSLRDLTGDPTKHMHRVYFKIFGVTGNKAIAQYYGHDTTREFMKSQIRRRRSRIDAILDVKTQDGHKIRTKAMVLTAYRANTKQKSDIRKKMEEIIKAMAKEKTFPQYVQAMLFGEMAEKIKNECKKIFPIKNVIIYKSEVLSLAKKEENEGFVKEAEEETAEAQE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1996-10-01" modified="2022-08-03" version="99" xmlns="http://uniprot.org/uniprot">
    <accession>P54110</accession>
    <name>RS14Z_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">30S ribosomal protein S14 type Z</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rps14</name>
        <name type="ordered locus">MJ0469.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="unpublished observations" date="1996-08">
            <authorList>
                <person name="Veuthey A.-L."/>
                <person name="Bairoch A."/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="1">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="status" value="NOT_ANNOTATED_CDS"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869970.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P54110"/>
    <dbReference type="SMR" id="P54110"/>
    <dbReference type="GeneID" id="8805615"/>
    <dbReference type="InParanoid" id="P54110"/>
    <dbReference type="OMA" id="HCFREIA"/>
    <dbReference type="OrthoDB" id="122051at2157"/>
    <dbReference type="PhylomeDB" id="P54110"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_S14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="40S_S29/30S_S14z"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_S14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_S14/S29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_S14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_S14_type-Z_arc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="PTHR12010"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0687">Ribonucleoprotein</keyword>
    <keyword id="KW-0689">Ribosomal protein</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0699">rRNA-binding</keyword>
    <keyword id="KW-0862">Zinc</keyword>
    <feature type="chain" id="PRO_0000130992" description="30S ribosomal protein S14 type Z">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <sequence length="53" mass="6192" checksum="975C67F2285BEFEF" modified="1996-10-01" version="1">MAKKPWKKKYGYGIRPCQRCGHVGPGLIRKYGLNLCRQCFREIAHKLGFKKLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="92" xmlns="http://uniprot.org/uniprot">
    <accession>P81310</accession>
    <name>Y61A_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0611.1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0611.1</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870116.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81310"/>
    <dbReference type="SMR" id="P81310"/>
    <dbReference type="STRING" id="243232.MJ_0611.1"/>
    <dbReference type="EnsemblBacteria" id="AAB98609">
        <property type="protein sequence ID" value="AAB98609"/>
        <property type="gene ID" value="MJ_0611.1"/>
    </dbReference>
    <dbReference type="GeneID" id="1451477"/>
    <dbReference type="KEGG" id="mja:MJ_0611.1"/>
    <dbReference type="eggNOG" id="arCOG07501">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1521855_0_0_2"/>
    <dbReference type="InParanoid" id="P81310"/>
    <dbReference type="OMA" id="FWKISMH"/>
    <dbReference type="OrthoDB" id="96272at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106958" description="Uncharacterized protein MJ0611.1">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="4"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="177"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="197" mass="23180" checksum="73FCFB65007C0692" modified="1998-07-15" version="1">MESIYLGNCWTPFNWIYAIYDVLVKILSIREIFQILSLMYLLFLYIGFLLISKFDISLSLAFFFPCVFWICWAKIKNETWDIPNRKNRLVPLIFTLIYLSILAIFWKNIFIIIFLVNVLVILIITKFWKISMHNYGLSAMAYLIYAFTNSIWLSTIYLILVIITGYARIYLKKHTVSQVIAGTILGISVNYILLNLI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>P81317</accession>
    <name>YB5B_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1155.2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1155.2</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870668.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="P81317"/>
    <dbReference type="STRING" id="243232.MJ_1155.2"/>
    <dbReference type="EnsemblBacteria" id="AAB99160">
        <property type="protein sequence ID" value="AAB99160"/>
        <property type="gene ID" value="MJ_1155.2"/>
    </dbReference>
    <dbReference type="GeneID" id="1452053"/>
    <dbReference type="KEGG" id="mja:MJ_1155.2"/>
    <dbReference type="HOGENOM" id="CLU_2695791_0_0_2"/>
    <dbReference type="OrthoDB" id="72210at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107195" description="Uncharacterized protein MJ1155.2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="73" mass="7963" checksum="FB33C20926CA058A" modified="1998-07-15" version="1">MILDKTLFSSLTFSLTVLFLLLLIPNLKGFGKLSAIIGGFIALIFQYFGYPSLGILFAGILSPIIILKIKSVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q57552</accession>
    <name>Y087_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative ABC transporter permease protein MJ0087</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0087</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Probably part of a binding-protein-dependent transport system. Probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64310">
        <property type="entry name" value="G64310"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869579.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57552"/>
    <dbReference type="SMR" id="Q57552"/>
    <dbReference type="STRING" id="243232.MJ_0087"/>
    <dbReference type="EnsemblBacteria" id="AAB98069">
        <property type="protein sequence ID" value="AAB98069"/>
        <property type="gene ID" value="MJ_0087"/>
    </dbReference>
    <dbReference type="GeneID" id="1450926"/>
    <dbReference type="KEGG" id="mja:MJ_0087"/>
    <dbReference type="eggNOG" id="arCOG01007">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013016_0_0_2"/>
    <dbReference type="InParanoid" id="Q57552"/>
    <dbReference type="OMA" id="AMVYWSF"/>
    <dbReference type="OrthoDB" id="36067at2157"/>
    <dbReference type="PhylomeDB" id="Q57552"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005887">
        <property type="term" value="C:integral component of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="PTHR30472"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="SSF81345"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000060304" description="Putative ABC transporter permease protein MJ0087">
        <location>
            <begin position="1"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="295"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="318"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="349" mass="38201" checksum="6F43FC95F29FE870" modified="1997-11-01" version="1">MDIPQKYKLYTKKKIIFGIILLITLFLSSIYALCVGDYKLTVNQVVNALMGYGKDDINLVIWNIRLPRIFAAIISGMSLAVAGAVMQCILRNPLASPFTMGISHGAMFGACFAIIMFGFGGAESTGRIFINNPYMITIFAFLGALIGVVVILLLAKLRGLTPEAMILAGVAMSSLFTAGTMLIQYFADDLQLAAMVYWTFGDLGRAIWTEIYIMAAVMIPSLIYFMYKRWDYNALEAGEETAKSLGVNTERTRLIGMLVASLLTSVNVAFLGIIGFVGLICPHIVRICIGGDYRFLIPISALFGAVLLLIADTFARTIIAPIVLPVGILTSFLGAPMFLYLLLKMYKRV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q57641</accession>
    <name>Y182_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0182</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0182</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64322">
        <property type="entry name" value="G64322"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57641"/>
    <dbReference type="STRING" id="243232.MJ_0182"/>
    <dbReference type="EnsemblBacteria" id="AAB98179">
        <property type="protein sequence ID" value="AAB98179"/>
        <property type="gene ID" value="MJ_0182"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0182"/>
    <dbReference type="eggNOG" id="arCOG12715">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1399755_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011646">
        <property type="entry name" value="KAP_P-loop"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07693">
        <property type="entry name" value="KAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106731" description="Uncharacterized protein MJ0182">
        <location>
            <begin position="1"/>
            <end position="194"/>
        </location>
    </feature>
    <sequence length="194" mass="22208" checksum="846E28002862EAF9" modified="1996-11-01" version="1">MSKLDKNEFICLKFDAWLYEKDDNLPYSLLEFIWDELEAKLNKDETITKEIKDKIKKLGKKSVNLWKNMVLGAINATNIKAGTSPITELSGIKINASFDGSKFVGYVVNASKEDENEEESYHKKVKELQNCFKELSKTLADNGKKLIIFIDELDRCEAENILNLLASIKLFFSLGGEDEDENKNDDEIKILFIL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q57669</accession>
    <name>VATB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>V-type ATP synthase beta chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>V-ATPase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">atpB</name>
        <name type="ordered locus">MJ0216</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text>Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal beta chain is a regulatory subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64327">
        <property type="entry name" value="A64327"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869712.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57669"/>
    <dbReference type="SMR" id="Q57669"/>
    <dbReference type="STRING" id="243232.MJ_0216"/>
    <dbReference type="EnsemblBacteria" id="AAB98199">
        <property type="protein sequence ID" value="AAB98199"/>
        <property type="gene ID" value="MJ_0216"/>
    </dbReference>
    <dbReference type="GeneID" id="1451066"/>
    <dbReference type="KEGG" id="mja:MJ_0216"/>
    <dbReference type="eggNOG" id="arCOG00865">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022916_0_0_2"/>
    <dbReference type="InParanoid" id="Q57669"/>
    <dbReference type="OMA" id="MLMMDSV"/>
    <dbReference type="OrthoDB" id="8899at2157"/>
    <dbReference type="PhylomeDB" id="Q57669"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005724">
        <property type="entry name" value="ATPase_A1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="PTHR43389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01041">
        <property type="entry name" value="ATP_syn_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000144656" description="V-type ATP synthase beta chain">
        <location>
            <begin position="1"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="465" mass="51375" checksum="F18924CD83450AC7" modified="1997-11-01" version="1">MATAASAIEYSSVKSIAGPLLIVEGVEGAAYGEIVEVICPDGEKRMGQVLEAREGLAVVQVFEGTTGLSTKDTRVRFTGRTAKIGVSMEMLGRIFNGAGKPIDGGPEIVPEKELDINGYPLNPVSRKVPSDFIQTGISTIDGMNTLVRGQKLPIFSGSGLPHNQLAAQIARQAKVRGEGEKFAVVFAAMGITSEEANFFMEEFRKTGALERAVVFINLADDPAIERILTPRIALTVAEYLAYEKDMHVLVILTDMTNYCEALREISAARNEVPGRRGYPGYMYTDLATIYERAGRVKGRTGTITQIPILTMPDDDITHPIPDLTGYITEGQIVLSRELHRKGIYPPVDVLPSLSRLAGNGQGPGKTREDHKKVVNQAYAAYAEGRSLRDLVAVVGEEALTDRDRAYLKFADEFEDKFVRQGKDEDRSIEETLDLLWELLAILPEEELKRVDRELIEKYHPKYRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57673</accession>
    <name>VATE_METJA</name>
    <protein>
        <recommendedName>
            <fullName>V-type ATP synthase subunit E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName>V-ATPase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">atpE</name>
        <name type="ordered locus">MJ0220</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64327">
        <property type="entry name" value="E64327"/>
    </dbReference>
    <dbReference type="PDB" id="2KK7">
        <property type="method" value="NMR"/>
        <property type="chains" value="A=2-50"/>
    </dbReference>
    <dbReference type="PDB" id="3LG8">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="4.10 A"/>
        <property type="chains" value="A/B=101-206"/>
    </dbReference>
    <dbReference type="PDBsum" id="2KK7"/>
    <dbReference type="PDBsum" id="3LG8"/>
    <dbReference type="AlphaFoldDB" id="Q57673"/>
    <dbReference type="BMRB" id="Q57673"/>
    <dbReference type="SMR" id="Q57673"/>
    <dbReference type="STRING" id="243232.MJ_0220"/>
    <dbReference type="EnsemblBacteria" id="AAB98203">
        <property type="protein sequence ID" value="AAB98203"/>
        <property type="gene ID" value="MJ_0220"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0220"/>
    <dbReference type="eggNOG" id="arCOG00869">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105846_1_0_2"/>
    <dbReference type="InParanoid" id="Q57673"/>
    <dbReference type="OMA" id="YAGNIDC"/>
    <dbReference type="PhylomeDB" id="Q57673"/>
    <dbReference type="EvolutionaryTrace" id="Q57673"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="PTHR45715"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="SSF81573"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0066">ATP synthesis</keyword>
    <keyword id="KW-0375">Hydrogen ion transport</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000117316" description="V-type ATP synthase subunit E">
        <location>
            <begin position="1"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="helix" evidence="3">
        <location>
            <begin position="6"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0007829" key="3">
        <source>
            <dbReference type="PDB" id="2KK7"/>
        </source>
    </evidence>
    <sequence length="206" mass="23137" checksum="5787427812D715A6" modified="1996-11-01" version="1">MKLMGVDKIKSKILDDAKAEANKIISEAEAEKAKILEKAKEEAEKRKAEILKKGEKEAEMTKSRIISEAKLEAKKKLLEAKEEIIEMAINKLKEELVKLPEQPEYKDKLIKLIKDGAISLGGGELIVRLNKRDMELIDDSTLWNLEKEVENATKKVTVLKKGEPVDIAGGCIIETADGLKSLDNSLEAIFNRNLNVIRARITEKLF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="136" xmlns="http://uniprot.org/uniprot">
    <accession>Q57679</accession>
    <name>IXTPA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="3">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Hypoxanthine/xanthine dNTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="4">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Nucleotide triphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0226</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2001" name="Nucleic Acids Res." volume="29" first="3099" last="3107">
            <title>Biochemical characterization of a novel hypoxanthine/xanthine dNTP pyrophosphatase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Chung J.H."/>
                <person name="Back J.H."/>
                <person name="Park Y.I."/>
                <person name="Han Y.S."/>
            </authorList>
            <dbReference type="PubMed" id="11452035"/>
            <dbReference type="DOI" id="10.1093/nar/29.14.3099"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="1999" name="Nat. Struct. Biol." volume="6" first="691" last="696">
            <title>Structure-based identification of a novel NTPase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Hwang K.Y."/>
                <person name="Chung J.H."/>
                <person name="Kim S.-H."/>
                <person name="Han Y.S."/>
                <person name="Cho Y."/>
            </authorList>
            <dbReference type="PubMed" id="10404228"/>
            <dbReference type="DOI" id="10.1038/10745"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF APOENZYME AND IN COMPLEX WITH ATP ANALOG</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>COFACTOR</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides xanthosine triphosphate (XTP), deoxyinosine triphosphate (dITP) and ITP (PubMed:10404228, PubMed:11452035). Probably functions as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions (PubMed:11452035). Shows very low activity on GTP or dGTP, both of which are hydrolyzed more than 100-fold less efficiently than XTP, and has nearly no activity toward the canonical nucleotides ATP, CTP, and TTP, and toward 6-N-hydroxylaminopurine deoxynucleoside triphosphate (dHAPTP) (PubMed:10404228, PubMed:11452035). Displays neither endonuclease nor 3'-exonuclease activities (PubMed:11452035).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H2O + XTP = diphosphate + H(+) + XMP</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>H2O + ITP = diphosphate + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1 2 3">Binds 1 Mg(2+) ion per subunit (By similarity). Magnesium ions are required for optimal activity, with Mn(2+), Zn(2+) and Ni(2+) supporting &lt;50% of the maximum rate (PubMed:11452035).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.1 mM for XTP (at pH 7.6 and 80 degrees Celsius)</KM>
            <KM evidence="2">0.15 mM for ITP (at pH 7.6 and 80 degrees Celsius)</KM>
            <KM evidence="2">1.11 mM for GTP (at pH 7.6 and 80 degrees Celsius)</KM>
            <KM evidence="2">1.13 mM for dGTP (at pH 7.6 and 80 degrees Celsius)</KM>
            <KM evidence="3">0.22 mM for XTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">0.25 mM for dITP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">0.24 mM for ITP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">2.12 mM for dGTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">1.94 mM for GTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">2.87 mM for 3'dGTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">2.78 mM for ddGTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">7.3 mM for dATP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">5.47 mM for dCTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">4.4 mM for dTTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">4.22 mM for dUTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">5.3 mM for 8-oxo-dGTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <KM evidence="3">3.3 mM for dHAPTP (at pH 10.5 and 80 degrees Celsius)</KM>
            <text evidence="2 3">kcat is 1009 sec(-1) with XTP as substrate. kcat is 911.7 sec(-1) with ITP as substrate. kcat is 97.6 sec(-1) with GTP as substrate. kcat is 96.6 sec(-1) with dGTP as substrate (at pH 7.6 and 80 degrees Celsius) (PubMed:10404228). kcat is 176.4 sec(-1) with XTP as substrate. kcat is 152.4 sec(-1) with dITP as substrate. kcat is 155.8 sec(-1) with ITP as substrate. kcat is 9.3 sec(-1) with dGTP as substrate. kcat is 9.1 sec(-1) with GTP as substrate. kcat is 8.2 sec(-1) with 3'dGTP as substrate. kcat is 3.3 sec(-1) with ddGTP as substrate. kcat is 0.44 sec(-1) with dATP as substrate. kcat is 0.44 sec(-1) with dCTP as substrate. kcat is 1.8 sec(-1) with dTTP as substrate. kcat is 5.8 sec(-1) with dUTP as substrate. kcat is 2.4 sec(-1) with 8-oxo-dGTP as substrate. kcat is 1.8 sec(-1) with dHAPTP as substrate (at pH 10.5 and 80 degrees Celsius) (PubMed:11452035).</text>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 10.5 with dITP as substrate. The reaction rates under neutral conditions are &lt;30% of maximum.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Optimum temperature is 80 degrees Celsius. At low temperature (30 degrees Celsius), shows about 5-fold decrease in activity compared to the maximum activity at 80 degrees Celsius. Is highly thermostable. The half-life of the protein at 95 degrees Celsius is about 200 minutes.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="sequence caution" evidence="6">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB98211" version="1"/>
        </conflict>
        <text>Extended N-terminus.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98211.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64328">
        <property type="entry name" value="C64328"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_064496428.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1B78">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.20 A"/>
        <property type="chains" value="A/B=1-185"/>
    </dbReference>
    <dbReference type="PDB" id="2MJP">
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
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                <person name="Woese C.R."/>
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            </authorList>
            <dbReference type="PubMed" id="8688087"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
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                <person name="Gocayne J.D."/>
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                <person name="Dougherty B.A."/>
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                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
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                <person name="Merrick J.M."/>
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                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
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                <person name="Hurst M.A."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">Binds 4 [4Fe-4S] clusters.</text>
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        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0249">Electron transport</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000159309" description="Uncharacterized protein MJ0264">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 1" evidence="2">
        <location>
            <begin position="16"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 2" evidence="2">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 3" evidence="2">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type 4" evidence="2">
        <location>
            <begin position="95"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00711"/>
        </source>
    </evidence>
    <sequence length="153" mass="17013" checksum="8550F880E9FD487F" modified="1996-11-01" version="1">MHQVKSFLLLAIDGEKMVVVNVGSCIGCRRCERSCPINGITFNEFPIKCMHCDRNPCLYACPENAIERINNKVVVIKDKCVGCGLCALACPFGAIRIDGVAIKCNGCYKRDVEICKEVCPTGAINNLEEILNNKIQNTVNKFNKLYYLYANAK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q57717</accession>
    <name>PORC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Pyruvate synthase subunit PorC</fullName>
            <ecNumber>1.2.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Pyruvate oxidoreductase gamma chain</fullName>
            <shortName>POR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>Pyruvic-ferredoxin oxidoreductase subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">porC</name>
        <name type="ordered locus">MJ0269</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>CoA + 2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate = acetyl-CoA + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text>Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64333">
        <property type="entry name" value="F64333"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869766.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57717"/>
    <dbReference type="SMR" id="Q57717"/>
    <dbReference type="STRING" id="243232.MJ_0269"/>
    <dbReference type="EnsemblBacteria" id="AAB98256">
        <property type="protein sequence ID" value="AAB98256"/>
        <property type="gene ID" value="MJ_0269"/>
    </dbReference>
    <dbReference type="GeneID" id="1451123"/>
    <dbReference type="KEGG" id="mja:MJ_0269"/>
    <dbReference type="eggNOG" id="arCOG01603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087284_2_0_2"/>
    <dbReference type="InParanoid" id="Q57717"/>
    <dbReference type="OMA" id="PDYVIVQ"/>
    <dbReference type="OrthoDB" id="95610at2157"/>
    <dbReference type="PhylomeDB" id="Q57717"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="SSF53323"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000099910" description="Pyruvate synthase subunit PorC">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <sequence length="178" mass="19202" checksum="3758EE7EC70FAEE1" modified="1996-11-01" version="1">MIEIRFHGRGGQGAVTAAQILAKAAFYDGKFCQAFPFFGVERRGAPVMAFTRIDDKKITLRCQIYEPDYVIVQDATLLESVNVVEGLKKDGAVVINTVKDDLDLGYKTYTIDATGIALDVLGVPIVNTAMVGAFAGVTGIVSIESVKKAILDTFKGKLGEKNAKAAEVAYNEMLKKYG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q57724</accession>
    <name>KORA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>2-oxoglutarate synthase subunit KorA</fullName>
            <ecNumber>1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>2-ketoglutarate oxidoreductase alpha chain</fullName>
            <shortName>KOR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>2-oxoglutarate-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">korA</name>
        <name type="ordered locus">MJ0276</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2-oxoglutarate + CoA + 2 oxidized [2Fe-2S]-[ferredoxin] = CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin] + succinyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64334">
        <property type="entry name" value="E64334"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57724"/>
    <dbReference type="SMR" id="Q57724"/>
    <dbReference type="STRING" id="243232.MJ_0276"/>
    <dbReference type="EnsemblBacteria" id="AAB98264">
        <property type="protein sequence ID" value="AAB98264"/>
        <property type="gene ID" value="MJ_0276"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0276"/>
    <dbReference type="eggNOG" id="arCOG01607">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017038_0_1_2"/>
    <dbReference type="InParanoid" id="Q57724"/>
    <dbReference type="OMA" id="DFIQGDE"/>
    <dbReference type="PhylomeDB" id="Q57724"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901576">
        <property type="term" value="P:organic substance biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProt"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="SSF52518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="SSF52922"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000099938" description="2-oxoglutarate synthase subunit KorA">
        <location>
            <begin position="1"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <sequence length="366" mass="41060" checksum="7C5E41EAD9EB6E78" modified="1996-11-01" version="1">MKVEFMQGNQACAKGAIKAGCRFFAGYPITPSTEIAEAMARELPKVGGYYIQMEDEIGSIAAVIGASWGGLKAMTATSGPGFSLMQENIGFAYMTETPCVVVDIQRGGPSTGQPTMASQGDMMQCRWGSHGDYEVIALAPSSVQEMYDFTIMAFNYAEKYRIPVFVMADEIVGHMREKVILHDNIEIINRKKPEEKPCKKPYPFDKLIPEMPVFGEGYNVHITGLTHDERGYPDVSPETHDKLVRRIVNKIRKNKDEIIKWEGENLDAEIVFVCYGSPSRTVKHAVRNLREKGLDVGYIRLITVYPFPDDLLKKLKAKKVVVPEMNLGQIYYEVERVCKKAEEVILVDKIGGELHRPEELERAVLG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57732</accession>
    <name>Y284_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0284</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0284</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64335">
        <property type="entry name" value="E64335"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57732"/>
    <dbReference type="SMR" id="Q57732"/>
    <dbReference type="STRING" id="243232.MJ_0284"/>
    <dbReference type="EnsemblBacteria" id="AAB98272">
        <property type="protein sequence ID" value="AAB98272"/>
        <property type="gene ID" value="MJ_0284"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0284"/>
    <dbReference type="eggNOG" id="arCOG00910">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074702_1_0_2"/>
    <dbReference type="InParanoid" id="Q57732"/>
    <dbReference type="OMA" id="DVVYSIH"/>
    <dbReference type="PhylomeDB" id="Q57732"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106772" description="Uncharacterized protein MJ0284">
        <location>
            <begin position="1"/>
            <end position="219"/>
        </location>
    </feature>
    <sequence length="219" mass="25139" checksum="04DD0C9ACBF4E51B" modified="1996-11-01" version="1">MIKKKHLEMMLDSLKRHPNPKADLEQYTIDGKLAADILFFAVNDFYNNVVIDLGCGTGRLAIGSKILGAKRAIGIDIDRESIEAAKENAKKLNVDVDFYCMDIRDVDDEFLNNVLGEDRDLKRVVIQNPPFGAQKKHADRVFLDKALEIGDIIYTIHNYPTKDFVIKYVEDKGGKITHIYEAFFRIPAIYEFHKKKVVEIPVVIFRIEKLGFETVFNFL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="67" xmlns="http://uniprot.org/uniprot">
    <accession>Q57776</accession>
    <name>Y330_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0330</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0330</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64341">
        <property type="entry name" value="B64341"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57776"/>
    <dbReference type="STRING" id="243232.MJ_0330"/>
    <dbReference type="EnsemblBacteria" id="AAB98318">
        <property type="protein sequence ID" value="AAB98318"/>
        <property type="gene ID" value="MJ_0330"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0330"/>
    <dbReference type="eggNOG" id="arCOG09550">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_512539_0_0_2"/>
    <dbReference type="OMA" id="WTRFPTP"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106799" description="Uncharacterized protein MJ0330">
        <location>
            <begin position="1"/>
            <end position="549"/>
        </location>
    </feature>
    <sequence length="549" mass="64471" checksum="CB4A8859FFC6AF99" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q57790</accession>
    <name>Y344_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0344</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0344</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64342">
        <property type="entry name" value="H64342"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869842.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57790"/>
    <dbReference type="STRING" id="243232.MJ_0344"/>
    <dbReference type="EnsemblBacteria" id="AAB98334">
        <property type="protein sequence ID" value="AAB98334"/>
        <property type="gene ID" value="MJ_0344"/>
    </dbReference>
    <dbReference type="GeneID" id="1451200"/>
    <dbReference type="KEGG" id="mja:MJ_0344"/>
    <dbReference type="eggNOG" id="arCOG09648">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1492986_0_0_2"/>
    <dbReference type="OMA" id="DESKHWI"/>
    <dbReference type="OrthoDB" id="140144at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106814" description="Uncharacterized protein MJ0344">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <sequence length="180" mass="19402" checksum="9EF646A626CDF3E2" modified="1996-11-01" version="1">MAVLASSTEPEPTESLLQQTNELLIIGKGAIVYKEFTTGVDARIIDSQTIGIGIPFNYYPFMCMVLDDAGNGIPMQEFTSAPGSLTVKVAATLDSTKTYKIIAFLIGDSMKIITSIQSQSGTVTEIAPGFGIFKVIYNDTEGTDTLTINYDDGTSETITVDKSNLWTVLPMQLPDAERVA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q57829</accession>
    <name>CS3HD_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CRISPR-associated endonuclease Cas3-HD</fullName>
            <ecNumber>3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>CRISPR-associated ssDNA/ssRNA endonuclease Cas3-HD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cas3</name>
        <name type="synonym">cas3''</name>
        <name type="ordered locus">MJ0384</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2011" name="EMBO J." volume="30" first="4616" last="4627">
            <title>Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference.</title>
            <authorList>
                <person name="Beloglazova N."/>
                <person name="Petit P."/>
                <person name="Flick R."/>
                <person name="Brown G."/>
                <person name="Savchenko A."/>
                <person name="Yakunin A.F."/>
            </authorList>
            <dbReference type="PubMed" id="22009198"/>
            <dbReference type="DOI" id="10.1038/emboj.2011.377"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS)</scope>
        <scope>FUNCTION AS A NUCLEASE</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATES</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>SUBUNIT</scope>
        <scope>MUTAGENESIS OF HIS-20; HIS-66; ASP-67; HIS-91; HIS-123; HIS-124 AND ASP-219</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 plus Cascade participate in CRISPR interference, the third stage of CRISPR immunity. Acts as a ssDNA and ssRNA nuclease, with both endo- and 3' to 5' exonuclease activities, acting on substrates with free single-stranded 3' ends. Double-stranded nucleic acids are not substrates. Activity is higher for DNA than RNA. Templates include R-loops (a bubble-like structure formed when ssRNA replaces one strand in a dsDNA, such as crRNA is thought to form with CRISPR target DNA), circular ssDNA, 2',3'-cAMP and 2',3'-cGMP. Probably generates 3'-phosphate and 5'-hydroxyl ends. In the presence of the Cas3 helicase and ATP dsDNA templates are also degraded.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="2">ssDNase activity requires Mg(2+), ssRNase activity does not require cations. A second loosely associated metal ion is visible in the crystal structure.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="2">Both ssDNase and ssRNase are inhibited by EDTA.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <phDependence>
            <text evidence="2">Optimum pH is 7-9, varying somewhat depending on the substrate.</text>
        </phDependence>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="domain">
        <text>Proteins of this family have an N-terminal nuclease domain and a C-terminal helicase/ATPase domain. In some CRISPR/Cas systems the domains are swapped, in others they are encoded separately.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CRISPR-associated nuclease Cas3-HD family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64347">
        <property type="entry name" value="H64347"/>
    </dbReference>
    <dbReference type="PDB" id="3S4L">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-244"/>
    </dbReference>
    <dbReference type="PDBsum" id="3S4L"/>
    <dbReference type="AlphaFoldDB" id="Q57829"/>
    <dbReference type="SMR" id="Q57829"/>
    <dbReference type="STRING" id="243232.MJ_0384"/>
    <dbReference type="DNASU" id="1451241"/>
    <dbReference type="EnsemblBacteria" id="AAB98379">
        <property type="protein sequence ID" value="AAB98379"/>
        <property type="gene ID" value="MJ_0384"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0384"/>
    <dbReference type="eggNOG" id="arCOG01442">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1052127_0_0_2"/>
    <dbReference type="OMA" id="HEPILMG"/>
    <dbReference type="PhylomeDB" id="Q57829"/>
    <dbReference type="EvolutionaryTrace" id="Q57829"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006483">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038257">
        <property type="entry name" value="CRISPR-assoc_Cas3_HD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18019">
        <property type="entry name" value="HD_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01596">
        <property type="entry name" value="cas3_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51643">
        <property type="entry name" value="HD_CAS3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0051">Antiviral defense</keyword>
    <keyword id="KW-0255">Endonuclease</keyword>
    <keyword id="KW-0269">Exonuclease</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106847" description="CRISPR-associated endonuclease Cas3-HD">
        <location>
            <begin position="1"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="HD Cas3-type" evidence="1">
        <location>
            <begin position="10"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="binding site">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Loss of all nuclease activity." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of all nuclease activity." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="66"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of all nuclease activity." evidence="2">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Very little nuclease activity." evidence="2">
        <original>D</original>
        <variation>E</variation>
        <location>
            <position position="67"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Very little ssDNA exonuclease activity." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="91"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Very little nuclease activity." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Very little nuclease activity." evidence="2">
        <original>H</original>
        <variation>A</variation>
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of all nuclease activity." evidence="2">
        <original>D</original>
        <variation>A</variation>
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="10"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="17"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="32"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="36"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="44"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="52"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="68"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="strand" evidence="4">
        <location>
            <begin position="82"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="110"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="142"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="159"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="181"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="turn" evidence="4">
        <location>
            <begin position="201"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="helix" evidence="4">
        <location>
            <begin position="204"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="22009198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0007829" key="4">
        <source>
            <dbReference type="PDB" id="3S4L"/>
        </source>
    </evidence>
    <sequence length="244" mass="28406" checksum="09CE6E74BB8621C3" modified="1996-11-01" version="1">MILGEIMEVLAFKNQSLIDHVNDMVKYWERIKYRYLKTIKRALEALNIKLDIEKVDEFMKILIKLHDIGKASKIYQRAIINDQEKLMGFRHELVSAYYTYHILLKKFGDKNLAFIGALTVMLHHEPIIMGQIRNLKKKELTAEVVLDKLKKFDGMIEDFEDLIKKLIGYSIGDIIKNDSNKDDIIRFVIEMSVRARHTPNSEKLRFIVGTLLLPLVMCDYKGAESREGKAPKFAEVLEVESYVI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="81" xmlns="http://uniprot.org/uniprot">
    <accession>Q57844</accession>
    <name>Y401_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0401</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0401</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64350">
        <property type="entry name" value="A64350"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869900.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57844"/>
    <dbReference type="SMR" id="Q57844"/>
    <dbReference type="STRING" id="243232.MJ_0401"/>
    <dbReference type="EnsemblBacteria" id="AAB98394">
        <property type="protein sequence ID" value="AAB98394"/>
        <property type="gene ID" value="MJ_0401"/>
    </dbReference>
    <dbReference type="GeneID" id="1451261"/>
    <dbReference type="KEGG" id="mja:MJ_0401"/>
    <dbReference type="eggNOG" id="arCOG06573">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2820824_0_0_2"/>
    <dbReference type="OMA" id="VIEVPYG"/>
    <dbReference type="OrthoDB" id="128477at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106853" description="Uncharacterized protein MJ0401">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <sequence length="66" mass="7416" checksum="69E0B0E5B8CF5B94" modified="1996-11-01" version="1">MEMKRFLCAKCQKVIEVPYGVPKPDVCPYCGAPATFIHRIDAGGRGLGPGRGRRCGMRMMGRFRRE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q57849</accession>
    <name>NK_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nucleoside kinase</fullName>
            <shortName evidence="3">NK</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">ATP-dependent nucleoside monophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Cytidine kinase</fullName>
            <ecNumber evidence="2">2.7.1.213</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Guanosine-inosine kinase</fullName>
            <ecNumber evidence="2">2.7.1.73</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0406</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2006" name="Acta Crystallogr. D" volume="62" first="1085" last="1097">
            <title>Structure of Methanocaldococcus jannaschii nucleoside kinase: an archaeal member of the ribokinase family.</title>
            <authorList>
                <person name="Arnfors L."/>
                <person name="Hansen T."/>
                <person name="Schonheit P."/>
                <person name="Ladenstein R."/>
                <person name="Meining W."/>
            </authorList>
            <dbReference type="PubMed" id="16929110"/>
            <dbReference type="DOI" id="10.1107/S0907444906024826"/>
        </citation>
        <scope>PROTEIN SEQUENCE</scope>
        <scope>X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF APOENZYME AND IN COMPLEX WITH ATP ANALOG AND ADENOSINE</scope>
        <scope>COFACTOR</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>SUBUNIT</scope>
        <scope>REACTION MECHANISM</scope>
        <scope>ACTIVE SITE</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2007" name="Extremophiles" volume="11" first="105" last="114">
            <title>The phosphofructokinase-B (MJ0406) from Methanocaldococcus jannaschii represents a nucleoside kinase with a broad substrate specificity.</title>
            <authorList>
                <person name="Hansen T."/>
                <person name="Arnfors L."/>
                <person name="Ladenstein R."/>
                <person name="Schoenheit P."/>
            </authorList>
            <dbReference type="PubMed" id="17021658"/>
            <dbReference type="DOI" id="10.1007/s00792-006-0018-1"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>COFACTOR</scope>
        <scope>SUBUNIT</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of a wide range of nucleosides to yield nucleoside monophosphates. Shows the highest activity for inosine, guanosine and cytidine, but very poor kinase activity with adenosine, thymidine, uridine and xanthosine. ATP is the best phosphate donor, but can also use ITP and GTP. Shows extremely low activity with fructose-6-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + cytidine = ADP + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.213"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + guanosine = ADP + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27710"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16750"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + inosine = ADP + H(+) + IMP</text>
            <dbReference type="Rhea" id="RHEA:21140"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="2">Can use Mg(2+) and Mn(2+) with equal efficiency in vitro, and to a lesser extent, Ni(2+).</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="2">0.25 mM for ATP (at 70 degrees Celsius)</KM>
            <KM evidence="2">17 uM for cytidine (at 50 degrees Celsius)</KM>
            <KM evidence="2">20 uM for cytidine (at 70 degrees Celsius)</KM>
            <KM evidence="2">20 uM for inosine (at 70 degrees Celsius)</KM>
            <KM evidence="2">21 uM for inosine (at 50 degrees Celsius)</KM>
            <KM evidence="2">62 uM for guanosine (at 70 degrees Celsius)</KM>
            <KM evidence="2">78 uM for guanosine (at 50 degrees Celsius)</KM>
            <KM evidence="2">160 uM for adenosine (at 70 degrees Celsius)</KM>
            <KM evidence="2">180 uM for uridine (at 50 degrees Celsius)</KM>
            <KM evidence="2">200 uM for uridine (at 70 degrees Celsius)</KM>
            <KM evidence="2">230 uM for adenosine (at 50 degrees Celsius)</KM>
            <KM evidence="2">300 uM for ribose (at 70 degrees Celsius)</KM>
            <KM evidence="2">710 uM for xanthosine (at 50 degrees Celsius)</KM>
            <KM evidence="2">900 uM for thymidine (at 50 degrees Celsius)</KM>
            <KM evidence="2">1000 uM for thymidine (at 70 degrees Celsius)</KM>
            <KM evidence="2">1800 uM for fructose (at 70 degrees Celsius)</KM>
            <KM evidence="2">2200 uM for 2-deoxy-adenosine (at 70 degrees Celsius)</KM>
            <KM evidence="2">2500 uM for 2-deoxy-adenosine (at 50 degrees Celsius)</KM>
            <Vmax evidence="2">120.0 umol/min/mg enzyme with guanosine as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">75.0 umol/min/mg enzyme with inosine as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">70.0 umol/min/mg enzyme with cytidine as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">29.3 umol/min/mg enzyme with guanosine as substrate (at 50 degrees Celsius)</Vmax>
            <Vmax evidence="2">18.8 umol/min/mg enzyme with inosine as substrate (at 50 degrees Celsius)</Vmax>
            <Vmax evidence="2">9.0 umol/min/mg enzyme with cytidine as substrate (at 50 degrees Celsius)</Vmax>
            <Vmax evidence="2">4.0 umol/min/mg enzyme with adenosine as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">1.0 umol/min/mg enzyme with adenosine as substrate (at 50 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.25 umol/min/mg enzyme with uridine as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.6 umol/min/mg enzyme with xanthosine as substrate (at 50 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.6 umol/min/mg enzyme with ribose as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.2 umol/min/mg enzyme with 2-deoxy-adenosine as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.08 umol/min/mg enzyme with thymidine as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.05 umol/min/mg enzyme with 2-deoxy-adenosine as substrate (at 50 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.04 umol/min/mg enzyme with uridine as substrate (at 50 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.03 umol/min/mg enzyme with fructose as substrate (at 70 degrees Celsius)</Vmax>
            <Vmax evidence="2">0.01 umol/min/mg enzyme with thymidine as substrate (at 50 degrees Celsius)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="2">Optimum pH is 7.0. 50% of activity is found at pH 6.1 and 8.2.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="2">Optimum temperature is 85 degrees Celsius. Thermostable.</text>
        </temperatureDependence>
    </comment>
    <comment type="subunit">
        <text evidence="1 2">Homodimer.</text>
    </comment>
    <comment type="mass spectrometry" mass="33937.0" method="Electrospray" evidence="1"/>
    <comment type="similarity">
        <text evidence="4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.213" evidence="2"/>
    <dbReference type="EC" id="2.7.1.73" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64350">
        <property type="entry name" value="F64350"/>
    </dbReference>
    <dbReference type="PDB" id="2C49">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.92 A"/>
        <property type="chains" value="A/B=1-302"/>
    </dbReference>
    <dbReference type="PDB" id="2C4E">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=1-302"/>
    </dbReference>
    <dbReference type="PDBsum" id="2C49"/>
    <dbReference type="PDBsum" id="2C4E"/>
    <dbReference type="AlphaFoldDB" id="Q57849"/>
    <dbReference type="SMR" id="Q57849"/>
    <dbReference type="STRING" id="243232.MJ_0406"/>
    <dbReference type="EnsemblBacteria" id="AAB98396">
        <property type="protein sequence ID" value="AAB98396"/>
        <property type="gene ID" value="MJ_0406"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0406"/>
    <dbReference type="eggNOG" id="arCOG00014">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027634_5_2_2"/>
    <dbReference type="InParanoid" id="Q57849"/>
    <dbReference type="OMA" id="SQQIARM"/>
    <dbReference type="PhylomeDB" id="Q57849"/>
    <dbReference type="BRENDA" id="2.7.1.B20">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57849"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106366">
        <property type="term" value="F:guanosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008906">
        <property type="term" value="F:inosine kinase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019206">
        <property type="term" value="F:nucleoside kinase activity"/>
        <property type="evidence" value="ECO:0000314"/>
        <property type="project" value="UniProtKB"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016310">
        <property type="term" value="P:phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="SSF53613"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0418">Kinase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000080152" description="Nucleoside kinase">
        <location>
            <begin position="2"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <begin position="111"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <begin position="214"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1 6">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="5">
        <location>
            <position position="250"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="5"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="15"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="37"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="58"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="turn" evidence="8">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="73"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="98"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="109"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="116"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="131"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="141"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="155"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="162"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="170"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="181"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="201"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="209"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="216"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="220"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="228"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="strand" evidence="8">
        <location>
            <begin position="280"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="helix" evidence="8">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="16929110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17021658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="17021658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="16929110"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="2C49"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="2C49"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="8">
        <source>
            <dbReference type="PDB" id="2C4E"/>
        </source>
    </evidence>
    <sequence length="302" mass="33920" checksum="6A535606CC7D3260" modified="1996-11-01" version="1">MGGKMEKITCVGHTALDYIFNVEKFPEPNTSIQIPSARKYYGGAAANTAVGIKKLGVNSELLSCVGYDFKNSGYERYLKNLDINISKLYYSEEEETPKAWIFTDKDNNQITFFLWGAAKHYKELNPPNFNTEIVHIATGDPEFNLKCAKKAYGNNLVSFDPGQDLPQYSKEMLLEIIEHTNFLFMNKHEFERASNLLNFEIDDYLERVDALIVTKGSKGSVIYTKDKKIEIPCIKAGKVIDPTGAGDSYRAGFLSAYVKGYDLEKCGLIGAATASFVVEAKGCQTNLPTWDKVVERLEKHRI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="121" xmlns="http://uniprot.org/uniprot">
    <accession>Q57854</accession>
    <name>HIS6_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Imidazole glycerol phosphate synthase subunit HisF</fullName>
            <ecNumber>4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>IGP synthase cyclase subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>IGP synthase subunit HisF</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>ImGP synthase subunit HisF</fullName>
            <shortName>IGPS subunit HisF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hisF</name>
        <name type="ordered locus">MJ0411</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HisA/HisF family.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64351">
        <property type="entry name" value="C64351"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869910.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57854"/>
    <dbReference type="SMR" id="Q57854"/>
    <dbReference type="STRING" id="243232.MJ_0411"/>
    <dbReference type="EnsemblBacteria" id="AAB98400">
        <property type="protein sequence ID" value="AAB98400"/>
        <property type="gene ID" value="MJ_0411"/>
    </dbReference>
    <dbReference type="GeneID" id="1451271"/>
    <dbReference type="KEGG" id="mja:MJ_0411"/>
    <dbReference type="eggNOG" id="arCOG00617">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048577_4_0_2"/>
    <dbReference type="InParanoid" id="Q57854"/>
    <dbReference type="OMA" id="IFHYKET"/>
    <dbReference type="OrthoDB" id="57066at2157"/>
    <dbReference type="PhylomeDB" id="Q57854"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04731">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01013">
        <property type="entry name" value="HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004651">
        <property type="entry name" value="HisF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="SSF51366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00735">
        <property type="entry name" value="hisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0368">Histidine biosynthesis</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142279" description="Imidazole glycerol phosphate synthase subunit HisF">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="11"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="272" mass="30168" checksum="84943CA540A0FECB" modified="1996-11-01" version="1">MLTKRIIPCLDIKDGRVVKGTKFLNLRDAGDPVELAQYYDDEGADELVFLDITASAEKRDIIIDVVERTAEKVFIPLTVGGGIKSIEDFRRILRAGADKVSINTAAVKNPNLIKEASEIFGSQCVVVAIDAKRHYVNEDEIDKINKNVVKVEDGYCWFEVYIYGGRKETGIDAINWAKKVEELGAGEILLTSIDKDGTKSGYDLILTKEISKSVKLPVIASGGCGKPEHVYEAFVYGKADAALMAGILHYREYTIEEIKKYCADRGIPMRLL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="94" xmlns="http://uniprot.org/uniprot">
    <accession>Q57860</accession>
    <name>Y417_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0417</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0417</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64352">
        <property type="entry name" value="A64352"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869916.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57860"/>
    <dbReference type="SMR" id="Q57860"/>
    <dbReference type="STRING" id="243232.MJ_0417"/>
    <dbReference type="EnsemblBacteria" id="AAB98404">
        <property type="protein sequence ID" value="AAB98404"/>
        <property type="gene ID" value="MJ_0417"/>
    </dbReference>
    <dbReference type="GeneID" id="1451277"/>
    <dbReference type="KEGG" id="mja:MJ_0417"/>
    <dbReference type="eggNOG" id="arCOG00302">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072983_0_0_2"/>
    <dbReference type="InParanoid" id="Q57860"/>
    <dbReference type="OMA" id="WEVGNAY"/>
    <dbReference type="OrthoDB" id="85871at2157"/>
    <dbReference type="PhylomeDB" id="Q57860"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' exodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' exoribonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106863" description="Uncharacterized protein MJ0417">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <sequence length="237" mass="27454" checksum="7C97038BBA2E52AD" modified="1996-11-01" version="1">MKVDLHVHSIVSKCSLNPKGLLEKFCIKKNIVPAICDHNKLTKLNFAIPGEEIATNSGEFIGLFLTEEIPANLDLYEALDRVREQGALIYLPHPFDLNRRRSLAKFNVLEEREFLKYVHVVEVFNSRCRSIEPNLKALEYAEKYDFAMAFGSDAHFIWEVGNAYIKFSELNIEKPDDLSPKEFLNLLKIKTDELLKAKSNLLKNPWKTRWHYGKLGSKYNIALYSKVVKNVRRKLNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="153" xmlns="http://uniprot.org/uniprot">
    <accession>Q57872</accession>
    <name>DCDB_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">dCTP deaminase, dUMP-forming</fullName>
            <ecNumber evidence="2 3 4">3.5.4.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2 9">Bifunctional dCTP deaminase:dUTPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2 8">DCD-DUT</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">MjDCD-DUT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">dcd</name>
        <name type="ordered locus">MJ0430</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="20667" last="20672">
            <title>A bifunctional dCTP deaminase-dUTP nucleotidohydrolase from the hyperthermophilic archaeon Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Bjoernberg O."/>
                <person name="Neuhard J."/>
                <person name="Nyman P.O."/>
            </authorList>
            <dbReference type="PubMed" id="12670946"/>
            <dbReference type="DOI" id="10.1074/jbc.M213010200"/>
        </citation>
        <scope>PROTEIN SEQUENCE OF 1-10</scope>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="11100" last="11106">
            <title>The Methanococcus jannaschii dCTP deaminase is a bifunctional deaminase and diphosphatase.</title>
            <authorList>
                <person name="Li H."/>
                <person name="Xu H."/>
                <person name="Graham D.E."/>
                <person name="White R.H."/>
            </authorList>
            <dbReference type="PubMed" id="12538648"/>
            <dbReference type="DOI" id="10.1074/jbc.M212460200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>ACTIVITY REGULATION</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
        <scope>MASS SPECTROMETRY</scope>
        <scope>MUTAGENESIS OF ASP-135 AND GLU-145</scope>
    </reference>
    <reference evidence="12" key="4">
        <citation type="journal article" date="2003" name="J. Biol. Chem." volume="278" first="27916" last="27922">
            <title>Structure of the bifunctional dCTP deaminase-dUTPase from Methanocaldococcus jannaschii and its relation to other homotrimeric dUTPases.</title>
            <authorList>
                <person name="Johansson E."/>
                <person name="Bjoernberg O."/>
                <person name="Nyman P.O."/>
                <person name="Larsen S."/>
            </authorList>
            <dbReference type="PubMed" id="12756253"/>
            <dbReference type="DOI" id="10.1074/jbc.M304361200"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS)</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference evidence="13 14 15" key="5">
        <citation type="journal article" date="2003" name="J. Mol. Biol." volume="331" first="885" last="896">
            <title>Structural basis for recognition and catalysis by the bifunctional dCTP deaminase and dUTPase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Huffman J.L."/>
                <person name="Li H."/>
                <person name="White R.H."/>
                <person name="Tainer J.A."/>
            </authorList>
            <dbReference type="PubMed" id="12909016"/>
            <dbReference type="DOI" id="10.1016/S0022-2836(03)00789-7"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF APOENZYME AND IN COMPLEXES WITH DCTP AND DUTP</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference evidence="16 17" key="6">
        <citation type="submission" date="2006-08" db="PDB data bank">
            <title>Structural evidence for a concerted bifunctionality in dCTP deaminase-dUTPase from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Bynck J.H."/>
                <person name="Willemoes M."/>
                <person name="Larsen S."/>
                <person name="Johansson E."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF WILD-TYPE AND OF MUTANT ALA-145 IN COMPLEX WITH DUTP</scope>
    </reference>
    <reference evidence="18" key="7">
        <citation type="submission" date="2009-02" db="PDB data bank">
            <title>Pre-steady state kinetic and structural evidence for a concerted biofunctionality in dCTP deaminase-dUTPase from Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Siggaard J.H.B."/>
                <person name="Johansson E."/>
                <person name="Vognsen T."/>
                <person name="Helt S.S."/>
                <person name="Harris P."/>
                <person name="Larsen S."/>
                <person name="Willemoes M."/>
            </authorList>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.62 ANGSTROMS)</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate. It also acts as a dUTP diphosphatase with a lower affinity for dUTP than for dCTP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3 4">
            <text>dCTP + 2 H2O = diphosphate + dUMP + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:19205"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.5.4.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3 4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="3">Inhibited by dTTP.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">17.6 uM for dCTP (at 60 degrees Celsius)</KM>
            <KM evidence="3">263 uM for dUTP (at 60 degrees Celsius)</KM>
            <Vmax evidence="3">14.7 umol/min/mg enzyme with dCTP as substrate (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="3">23.9 umol/min/mg enzyme with dUTP as substrate (at 60 degrees Celsius)</Vmax>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 7.5.</text>
        </phDependence>
        <temperatureDependence>
            <text evidence="3">Retains over 70% of its activity after heating at 90 degrees Celsius for 10 min.</text>
        </temperatureDependence>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Homotrimer (PubMed:12756253, PubMed:12909016). Two trimers assemble into a hexamer by stacking on top of each other (PubMed:12909016).</text>
    </comment>
    <comment type="mass spectrometry" mass="23619.0" error="94.0" method="MALDI" evidence="3"/>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the dCTP deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.30" evidence="2 3 4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64353">
        <property type="entry name" value="F64353"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869929.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1OGH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.88 A"/>
        <property type="chains" value="A/B=1-204"/>
    </dbReference>
    <dbReference type="PDB" id="1PKH">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.42 A"/>
        <property type="chains" value="A/B=1-204"/>
    </dbReference>
    <dbReference type="PDB" id="1PKJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B=1-204"/>
    </dbReference>
    <dbReference type="PDB" id="1PKK">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.77 A"/>
        <property type="chains" value="A/B=1-204"/>
    </dbReference>
    <dbReference type="PDB" id="2HXB">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.55 A"/>
        <property type="chains" value="A=1-204"/>
    </dbReference>
    <dbReference type="PDB" id="2HXD">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A=1-204"/>
    </dbReference>
    <dbReference type="PDB" id="3GF0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.62 A"/>
        <property type="chains" value="A=1-204"/>
    </dbReference>
    <dbReference type="PDBsum" id="1OGH"/>
    <dbReference type="PDBsum" id="1PKH"/>
    <dbReference type="PDBsum" id="1PKJ"/>
    <dbReference type="PDBsum" id="1PKK"/>
    <dbReference type="PDBsum" id="2HXB"/>
    <dbReference type="PDBsum" id="2HXD"/>
    <dbReference type="PDBsum" id="3GF0"/>
    <dbReference type="AlphaFoldDB" id="Q57872"/>
    <dbReference type="SMR" id="Q57872"/>
    <dbReference type="STRING" id="243232.MJ_0430"/>
    <dbReference type="MoonProt" id="Q57872"/>
    <dbReference type="EnsemblBacteria" id="AAB98415">
        <property type="protein sequence ID" value="AAB98415"/>
        <property type="gene ID" value="MJ_0430"/>
    </dbReference>
    <dbReference type="GeneID" id="1451290"/>
    <dbReference type="KEGG" id="mja:MJ_0430"/>
    <dbReference type="eggNOG" id="arCOG04048">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087476_2_1_2"/>
    <dbReference type="InParanoid" id="Q57872"/>
    <dbReference type="OMA" id="PPIERIN"/>
    <dbReference type="OrthoDB" id="91282at2157"/>
    <dbReference type="PhylomeDB" id="Q57872"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-17894"/>
    <dbReference type="BRENDA" id="3.5.4.13">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.5.4.30">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="BRENDA" id="3.6.1.23">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q57872"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00667"/>
    </dbReference>
    <dbReference type="EvolutionaryTrace" id="Q57872"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033973">
        <property type="term" value="F:dCTP deaminase (dUMP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029054">
        <property type="entry name" value="dUTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00692">
        <property type="entry name" value="dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="SSF51283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0903">Direct protein sequencing</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000156029" description="dCTP deaminase, dUMP-forming">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="1 2">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="117"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 11">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 10 11">
        <location>
            <begin position="143"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 11">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 11">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2 11">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for bifunctional activity" evidence="2">
        <location>
            <begin position="132"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of activity." evidence="3">
        <original>D</original>
        <variation>N</variation>
        <location>
            <position position="135"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of dCTP deaminase activity, but retains 25% dUTP pyrophosphatase activity." evidence="3">
        <original>E</original>
        <variation>Q</variation>
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="helix" evidence="20">
        <location>
            <begin position="5"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="16"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="helix" evidence="20">
        <location>
            <begin position="24"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="32"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="38"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="strand" evidence="19">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="56"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="62"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="helix" evidence="20">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="89"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="110"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="helix" evidence="20">
        <location>
            <begin position="118"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="turn" evidence="20">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="125"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="153"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="160"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="strand" evidence="20">
        <location>
            <begin position="176"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="helix" evidence="21">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="P28248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12538648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="12670946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="5">
        <source>
            <dbReference type="PubMed" id="12756253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="6">
        <source>
            <dbReference type="PubMed" id="12909016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="7">
        <source>
            <dbReference type="PubMed" id="12538648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="8">
        <source>
            <dbReference type="PubMed" id="12909016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="9"/>
    <evidence type="ECO:0000305" key="10">
        <source>
            <dbReference type="PubMed" id="12909016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="11">
        <source ref="6"/>
    </evidence>
    <evidence type="ECO:0007744" key="12">
        <source>
            <dbReference type="PDB" id="1OGH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="13">
        <source>
            <dbReference type="PDB" id="1PKH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="14">
        <source>
            <dbReference type="PDB" id="1PKJ"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="15">
        <source>
            <dbReference type="PDB" id="1PKK"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="16">
        <source>
            <dbReference type="PDB" id="2HXB"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="17">
        <source>
            <dbReference type="PDB" id="2HXD"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="18">
        <source>
            <dbReference type="PDB" id="3GF0"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="19">
        <source>
            <dbReference type="PDB" id="1OGH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="20">
        <source>
            <dbReference type="PDB" id="1PKH"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="21">
        <source>
            <dbReference type="PDB" id="2HXD"/>
        </source>
    </evidence>
    <sequence length="204" mass="23432" checksum="1218368057723371" modified="1996-11-01" version="1">MILSDKDIIDYVTSKRIIIKPFNKDFVGPCSYDVTLGDEFIIYDDEVYDLSKELNYKRIKIKNSILVCPLNYNLTEEKINYFKEKYNVDYVVEGGVLGTTNEYIELPNDISAQYQGRSSLGRVFLTSHQTAGWIDAGFKGKITLEIVAFDKPVILYKNQRIGQLIFSKLLSPADVGYSERKTSKYAYQKSVMPSLIHLDNHKKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="95" xmlns="http://uniprot.org/uniprot">
    <accession>Q57879</accession>
    <name>Y437_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0437</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0437</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64354">
        <property type="entry name" value="E64354"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869936.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57879"/>
    <dbReference type="SMR" id="Q57879"/>
    <dbReference type="STRING" id="243232.MJ_0437"/>
    <dbReference type="EnsemblBacteria" id="AAB98425">
        <property type="protein sequence ID" value="AAB98425"/>
        <property type="gene ID" value="MJ_0437"/>
    </dbReference>
    <dbReference type="GeneID" id="1451297"/>
    <dbReference type="KEGG" id="mja:MJ_0437"/>
    <dbReference type="eggNOG" id="arCOG03076">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_173139_2_1_2"/>
    <dbReference type="InParanoid" id="Q57879"/>
    <dbReference type="OMA" id="MAYLYYA"/>
    <dbReference type="OrthoDB" id="125479at2157"/>
    <dbReference type="PhylomeDB" id="Q57879"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MbhD-like_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="DUF4040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106876" description="Uncharacterized protein MJ0437">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="79" mass="8717" checksum="5ECADEC8B82977C6" modified="1996-11-01" version="1">MEIIHYIVIIMTLLSSLASLLQRDLIKCIILSGFAGLCMAYLYYALLAPDVALTEAILGGAILPALFAFTVRRTQRIDE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="109" xmlns="http://uniprot.org/uniprot">
    <accession>Q57902</accession>
    <name>SUI1_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0463</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64357">
        <property type="entry name" value="G64357"/>
    </dbReference>
    <dbReference type="PDB" id="4MO0">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A=2-103"/>
    </dbReference>
    <dbReference type="PDB" id="5JB3">
        <property type="method" value="EM"/>
        <property type="resolution" value="5.34 A"/>
        <property type="chains" value="1=2-103"/>
    </dbReference>
    <dbReference type="PDB" id="5JBH">
        <property type="method" value="EM"/>
        <property type="resolution" value="5.34 A"/>
        <property type="chains" value="1=2-103"/>
    </dbReference>
    <dbReference type="PDBsum" id="4MO0"/>
    <dbReference type="PDBsum" id="5JB3"/>
    <dbReference type="PDBsum" id="5JBH"/>
    <dbReference type="AlphaFoldDB" id="Q57902"/>
    <dbReference type="SMR" id="Q57902"/>
    <dbReference type="STRING" id="243232.MJ_0463"/>
    <dbReference type="EnsemblBacteria" id="AAB98452">
        <property type="protein sequence ID" value="AAB98452"/>
        <property type="gene ID" value="MJ_0463"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0463"/>
    <dbReference type="eggNOG" id="arCOG04223">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_6_1_2"/>
    <dbReference type="InParanoid" id="Q57902"/>
    <dbReference type="OMA" id="TIRDSWI"/>
    <dbReference type="PhylomeDB" id="Q57902"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="SSF55159"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01158">
        <property type="entry name" value="SUI1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0810">Translation regulation</keyword>
    <feature type="chain" id="PRO_0000130580" description="Protein translation factor SUI1 homolog">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="28"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="40"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="turn" evidence="2">
        <location>
            <begin position="49"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="69"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="75"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="helix" evidence="2">
        <location>
            <begin position="97"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="strand" evidence="2">
        <location>
            <begin position="100"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="2">
        <source>
            <dbReference type="PDB" id="4MO0"/>
        </source>
    </evidence>
    <sequence length="103" mass="11732" checksum="766728FD824A809C" modified="1996-11-01" version="1">MMPEICPRCGLPKELCVCEEIAKEEQKIKIYVTKRRFGKLMTIIEGFDTSVIDLKELAKKLKDICACGGTVKDNTIELQGDHRKKVAEELVKMGFSRDSIEIR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q57925</accession>
    <name>AROA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="1">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="1">EPSP synthase</shortName>
            <shortName evidence="1">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">aroA</name>
        <name type="ordered locus">MJ0502</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the EPSP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57925"/>
    <dbReference type="SMR" id="Q57925"/>
    <dbReference type="STRING" id="243232.MJ_0502"/>
    <dbReference type="EnsemblBacteria" id="AAB98493">
        <property type="protein sequence ID" value="AAB98493"/>
        <property type="gene ID" value="MJ_0502"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0502"/>
    <dbReference type="eggNOG" id="arCOG04134">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024321_0_0_2"/>
    <dbReference type="InParanoid" id="Q57925"/>
    <dbReference type="OMA" id="YEDHRMA"/>
    <dbReference type="PhylomeDB" id="Q57925"/>
    <dbReference type="UniPathway" id="UPA00053"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:cellular amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="SSF55205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000088329" description="3-phosphoshikimate 1-carboxyvinyltransferase">
        <location>
            <begin position="1"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphoenolpyruvate" evidence="1">
        <location>
            <begin position="91"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="1">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="1">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="22"/>
            <end position="23"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="168"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="429" mass="47009" checksum="EE1D8C083B934D59" modified="1998-12-15" version="2">MYLLIVKKTDRLEGIVKAPPSKSYTHRAVIGASLADGVSRIINPLWGADCLSSVHGCRMLGANIELDKEKDEWIVKGGELKTPDNIIDIGNSGTTLRILTSIASQIPKGYAILTGDDSIRKRPMQPLLDALKQLNIEAFSSKLDGTAPIIVKSGKIYGNVVKIRGDISSQFITSLMMLLPFNKEDTEIILTSPLKSKPYIDITLDILNKFGIKIDKTDNGFLVYGNQKYKPIDYIVEGDYSSASYLIAAGVLINSNITIENLFANSKQGDKAIINIVKEMGADIKVKKDKVIIEGEYSLKGIDVDVKDIPDLVPTIAVLGCFAEGKTEIYNGEHVRLKECDRLRACAVELKKMGADIEEKPDGLIIRGVKKLKGAKLNTYHDHRLVMAFTIAGLKAEGETIIEGEEAVKISFPNFVDVMKSLGANIEVK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-06-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q57939</accession>
    <name>Y519_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0519</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0519</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64364">
        <property type="entry name" value="G64364"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57939"/>
    <dbReference type="SMR" id="Q57939"/>
    <dbReference type="STRING" id="243232.MJ_0519"/>
    <dbReference type="EnsemblBacteria" id="AAB98513">
        <property type="protein sequence ID" value="AAB98513"/>
        <property type="gene ID" value="MJ_0519"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0519"/>
    <dbReference type="eggNOG" id="arCOG08277">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_188101_0_0_2"/>
    <dbReference type="OMA" id="HKMEDIE"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011319">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaK"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036538">
        <property type="entry name" value="EhaK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106909" description="Uncharacterized protein MJ0519">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="84" mass="9680" checksum="51E95DA171C2F48A" modified="1996-11-01" version="1">MDKMKSYIYFFTIACIIAVIYCVLVNLLQINVIPVVLAFSLILILTISTINKKIAHKMEDIEVLFMLLVLAFFAYAIYKLYIPV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q57941</accession>
    <name>Y521_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0521</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0521</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64365">
        <property type="entry name" value="A64365"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57941"/>
    <dbReference type="STRING" id="243232.MJ_0521"/>
    <dbReference type="DNASU" id="1451385"/>
    <dbReference type="EnsemblBacteria" id="AAB98528">
        <property type="protein sequence ID" value="AAB98528"/>
        <property type="gene ID" value="MJ_0521"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0521"/>
    <dbReference type="eggNOG" id="arCOG05034">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2784017_0_0_2"/>
    <dbReference type="OMA" id="LICYILY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106910" description="Uncharacterized protein MJ0521">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="69" mass="7769" checksum="F9D0AAF9875D6451" modified="1996-11-01" version="1">MKNMDEERKYGLYSLIIGLLCVIGIVMLNGLICYVLYIIAVPSLLYGIGAFIIPKTRRKDAGKLPFRGY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="89" xmlns="http://uniprot.org/uniprot">
    <accession>Q57965</accession>
    <name>Y545_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0545</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0545</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64368">
        <property type="entry name" value="A64368"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57965"/>
    <dbReference type="STRING" id="243232.MJ_0545"/>
    <dbReference type="EnsemblBacteria" id="AAB98547">
        <property type="protein sequence ID" value="AAB98547"/>
        <property type="gene ID" value="MJ_0545"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0545"/>
    <dbReference type="eggNOG" id="arCOG05039">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1259103_0_0_2"/>
    <dbReference type="OMA" id="YVIFQWR"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106923" description="Uncharacterized protein MJ0545">
        <location>
            <begin position="1"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="251" mass="28349" checksum="ED36603E1C61DC67" modified="1996-11-01" version="1">MVGKMKKVIIPLLISLFIFLIPNYALNPEIIVTPEKCLVNNSVYVIFQWRAPYNVEDFNVTVLSDAVVFKNSTLYYAGVAEDAKVFHIFEGEAVTPGNHTINVQMSYIIDGTLIKKKFLLNISILTLPENIYVSYNNTYNRDEENTSLLENITKIFENTTNVTTPNSTNAIINETNITQNKTNISKNIDIGNITKANTTSQEKITQKFNNTSTQTIENVQKDKGNNWLMYGILGLIIGIVFGFVVMYIIKI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q57983</accession>
    <name>MT51_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative type II methyltransferase M.MJ0563P</fullName>
            <shortName evidence="2">M.MJ0563P</shortName>
            <ecNumber>2.1.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Cytosine-specific methyltransferase MJ0563</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0563</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2003" name="Nucleic Acids Res." volume="31" first="1805" last="1812">
            <title>A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.</title>
            <authorList>
                <person name="Roberts R.J."/>
                <person name="Belfort M."/>
                <person name="Bestor T."/>
                <person name="Bhagwat A.S."/>
                <person name="Bickle T.A."/>
                <person name="Bitinaite J."/>
                <person name="Blumenthal R.M."/>
                <person name="Degtyarev S.K."/>
                <person name="Dryden D.T."/>
                <person name="Dybvig K."/>
                <person name="Firman K."/>
                <person name="Gromova E.S."/>
                <person name="Gumport R.I."/>
                <person name="Halford S.E."/>
                <person name="Hattman S."/>
                <person name="Heitman J."/>
                <person name="Hornby D.P."/>
                <person name="Janulaitis A."/>
                <person name="Jeltsch A."/>
                <person name="Josephsen J."/>
                <person name="Kiss A."/>
                <person name="Klaenhammer T.R."/>
                <person name="Kobayashi I."/>
                <person name="Kong H."/>
                <person name="Krueger D.H."/>
                <person name="Lacks S."/>
                <person name="Marinus M.G."/>
                <person name="Miyahara M."/>
                <person name="Morgan R.D."/>
                <person name="Murray N.E."/>
                <person name="Nagaraja V."/>
                <person name="Piekarowicz A."/>
                <person name="Pingoud A."/>
                <person name="Raleigh E."/>
                <person name="Rao D.N."/>
                <person name="Reich N."/>
                <person name="Repin V.E."/>
                <person name="Selker E.U."/>
                <person name="Shaw P.C."/>
                <person name="Stein D.C."/>
                <person name="Stoddard B.L."/>
                <person name="Szybalski W."/>
                <person name="Trautner T.A."/>
                <person name="Van Etten J.L."/>
                <person name="Vitor J.M."/>
                <person name="Wilson G.G."/>
                <person name="Xu S.Y."/>
            </authorList>
            <dbReference type="PubMed" id="12654995"/>
            <dbReference type="DOI" id="10.1093/nar/gkg274"/>
        </citation>
        <scope>NOMENCLATURE</scope>
    </reference>
    <comment type="function">
        <text evidence="2">A putative methylase that may protect DNA from cleavage by an unknown endonuclease.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:13681"/>
            <dbReference type="Rhea" id="RHEA-COMP:11369"/>
            <dbReference type="Rhea" id="RHEA-COMP:11370"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:85452"/>
            <dbReference type="ChEBI" id="CHEBI:85454"/>
            <dbReference type="EC" id="2.1.1.37"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64370">
        <property type="entry name" value="C64370"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870067.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q57983"/>
    <dbReference type="SMR" id="Q57983"/>
    <dbReference type="STRING" id="243232.MJ_0563"/>
    <dbReference type="REBASE" id="3888">
        <property type="entry name" value="M.MjaORF563P"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB98555">
        <property type="protein sequence ID" value="AAB98555"/>
        <property type="gene ID" value="MJ_0563"/>
    </dbReference>
    <dbReference type="GeneID" id="1451428"/>
    <dbReference type="KEGG" id="mja:MJ_0563"/>
    <dbReference type="eggNOG" id="arCOG04157">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006958_2_2_2"/>
    <dbReference type="InParanoid" id="Q57983"/>
    <dbReference type="OMA" id="HPFTPRC"/>
    <dbReference type="OrthoDB" id="58937at2157"/>
    <dbReference type="PhylomeDB" id="Q57983"/>
    <dbReference type="PRO" id="PR:Q57983"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="SSF53335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0680">Restriction system</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000087916" description="Putative type II methyltransferase M.MJ0563P">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="SAM-dependent MTase C5-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="12654995"/>
        </source>
    </evidence>
    <sequence length="310" mass="36290" checksum="0F983B2193692D78" modified="1996-11-01" version="1">MNVIDLFSGCGGFSKGFLDENFRILGAIENFKPVVKTYLYNIKAPVWMDDIKRIPPKAFDEFIKNEKVDVIIGSPPCEPFTKANKLIKDNPLDRLYKDKVGRLVLYYIDYVNYFTQRNDDLIFVMENVPQIKEIKDELKKLFGDIGHKVYFNILRAEDYGNPSKRARMFISNIKLKPKKVDKLVVVEEALKDIPKDAKNHEIKKLSKEKVEMISKLKWGEALYRYRGKKKLMFNWYKLHPKKLAPTVKGRSRFIHPYEDRLLTVREQARLMSYPDDFVFFGGRDVQYNQIGESVPPILGRAIAKEIKKQL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="65" xmlns="http://uniprot.org/uniprot">
    <accession>Q58016</accession>
    <name>Y599_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0599</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0599</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64374">
        <property type="entry name" value="G64374"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870103.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58016"/>
    <dbReference type="STRING" id="243232.MJ_0599"/>
    <dbReference type="EnsemblBacteria" id="AAB98597">
        <property type="protein sequence ID" value="AAB98597"/>
        <property type="gene ID" value="MJ_0599"/>
    </dbReference>
    <dbReference type="GeneID" id="1451464"/>
    <dbReference type="KEGG" id="mja:MJ_0599"/>
    <dbReference type="HOGENOM" id="CLU_1369535_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000106951" description="Uncharacterized protein MJ0599">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <sequence length="199" mass="24196" checksum="52D6E8D92BF19E0A" modified="1996-11-01" version="1">MQIKPFEENKPYPEWDPDEPDKVPGIPNWNRNFGNIIIEFDKGTRTCYYHRKKKNKYDVECGDYRIKITYSQKNWNKKVVYDNDAWKWLVDCLDPTEINKFLCHLCKILDMNIDKDVYDYLINNVNFSVNNNDLRALLYYFWHRAIIEDRIYSQKKGYNGRKQVIGATYEVLKELRNNQNRVYLYEKCDEIYKMAKKKL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58091</accession>
    <name>Y678_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0678</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0678</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64384">
        <property type="entry name" value="F64384"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870183.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58091"/>
    <dbReference type="SMR" id="Q58091"/>
    <dbReference type="STRING" id="243232.MJ_0678"/>
    <dbReference type="EnsemblBacteria" id="AAB98673">
        <property type="protein sequence ID" value="AAB98673"/>
        <property type="gene ID" value="MJ_0678"/>
    </dbReference>
    <dbReference type="GeneID" id="1451544"/>
    <dbReference type="KEGG" id="mja:MJ_0678"/>
    <dbReference type="eggNOG" id="arCOG02264">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050976_0_0_2"/>
    <dbReference type="InParanoid" id="Q58091"/>
    <dbReference type="OMA" id="MAVGPEF"/>
    <dbReference type="OrthoDB" id="75041at2157"/>
    <dbReference type="PhylomeDB" id="Q58091"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="PTHR20992"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106986" description="Uncharacterized protein MJ0678">
        <location>
            <begin position="1"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="320" mass="35674" checksum="92BBB732DFBF7705" modified="1996-11-01" version="1">MKLRFIECHIPKHLFMGIDEIREWDGVIWANVKTNGTISTIQILTTLKDSEKIVDKLKEMYGGANYRVVVFEPTMTYPPIEEEEEKEEPERLIREELYNIASDIANLSKENMLMLILSTIVAIAGIYKDDVALLIASMIIAPLLGPNIALSLSITVADYKLALKSIKTLIAELIFVIILSMIAGHYLPISLDNPQIHSRITLDFWSIIIALSAGIAGSLSTVSNISSIAVGVMIAIALLPPLAVFGLLIGAGYVEQSFSALILFLINMIAINLSAIVIFSAYGISPYRWWKKEEARKYTLYAILLWVTLFIAIFVLIIYH</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q58103</accession>
    <name>Y691_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-binding protein MJ0691</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0691</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PDCD5 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64386">
        <property type="entry name" value="C64386"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870196.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58103"/>
    <dbReference type="SMR" id="Q58103"/>
    <dbReference type="STRING" id="243232.MJ_0691"/>
    <dbReference type="EnsemblBacteria" id="AAB98686">
        <property type="protein sequence ID" value="AAB98686"/>
        <property type="gene ID" value="MJ_0691"/>
    </dbReference>
    <dbReference type="GeneID" id="1451557"/>
    <dbReference type="KEGG" id="mja:MJ_0691"/>
    <dbReference type="eggNOG" id="arCOG04179">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_122978_3_0_2"/>
    <dbReference type="InParanoid" id="Q58103"/>
    <dbReference type="OMA" id="KPQFGEQ"/>
    <dbReference type="OrthoDB" id="130316at2157"/>
    <dbReference type="PhylomeDB" id="Q58103"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PTHR10840"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="SSF46950"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000121555" description="DNA-binding protein MJ0691">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <sequence length="109" mass="12892" checksum="DAB41A2366B8822C" modified="1996-11-01" version="1">MDVEEIKRKKLLELQKKLAEQQQQEEALLEAEMQKRALLRKILTPEARERLERIRLARPEFAEAVEVQLIQLAQLGRLPIPLSDEDFKALLERISALTKRKREIKIVRK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58117</accession>
    <name>Y706_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0706</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0706</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64388">
        <property type="entry name" value="B64388"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870212.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58117"/>
    <dbReference type="SMR" id="Q58117"/>
    <dbReference type="STRING" id="243232.MJ_0706"/>
    <dbReference type="TCDB" id="9.B.98.1.5">
        <property type="family name" value="the duf95 (duf95) family"/>
    </dbReference>
    <dbReference type="DNASU" id="1451574"/>
    <dbReference type="EnsemblBacteria" id="AAB98700">
        <property type="protein sequence ID" value="AAB98700"/>
        <property type="gene ID" value="MJ_0706"/>
    </dbReference>
    <dbReference type="GeneID" id="1451574"/>
    <dbReference type="KEGG" id="mja:MJ_0706"/>
    <dbReference type="eggNOG" id="arCOG01994">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099320_0_1_2"/>
    <dbReference type="OMA" id="IMFFALI"/>
    <dbReference type="OrthoDB" id="120839at2157"/>
    <dbReference type="PhylomeDB" id="Q58117"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002798">
        <property type="entry name" value="SpoIIM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01944">
        <property type="entry name" value="SpoIIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106998" description="Uncharacterized protein MJ0706">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="214" mass="24497" checksum="BA53797337D0304F" modified="1996-11-01" version="1">MRDAYLMMVLMDALKEIFDLKEILKSPIRNKKVILFVSLVFILSLVLLYILVVNIKYFSYLGDIIFQNFQKHVENLKITLNEDNLHIILAIWKNNLTVCILNYILGIFSLFVIAVNSYILSYVLYKFGAESFIYLVLPHGIIEIPALILSASGGVLFNMGLVNFLINIKFGTKREVLYYIKESLKLLILSIILFIVAGIVEGTITFKIAKIMFS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58174</accession>
    <name>Y764_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0764</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0764</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64395">
        <property type="entry name" value="D64395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870269.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="2B8M">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.70 A"/>
        <property type="chains" value="A=1-116"/>
    </dbReference>
    <dbReference type="PDBsum" id="2B8M"/>
    <dbReference type="AlphaFoldDB" id="Q58174"/>
    <dbReference type="SMR" id="Q58174"/>
    <dbReference type="STRING" id="243232.MJ_0764"/>
    <dbReference type="EnsemblBacteria" id="AAB98760">
        <property type="protein sequence ID" value="AAB98760"/>
        <property type="gene ID" value="MJ_0764"/>
    </dbReference>
    <dbReference type="GeneID" id="1451641"/>
    <dbReference type="KEGG" id="mja:MJ_0764"/>
    <dbReference type="eggNOG" id="arCOG02992">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2115513_0_0_2"/>
    <dbReference type="OMA" id="TEHVQIN"/>
    <dbReference type="OrthoDB" id="109106at2157"/>
    <dbReference type="EvolutionaryTrace" id="Q58174"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006045">
        <property type="entry name" value="Cupin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00190">
        <property type="entry name" value="Cupin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="SSF51182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107022" description="Uncharacterized protein MJ0764">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="16"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="48"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="67"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="74"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="82"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="103"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="2B8M"/>
        </source>
    </evidence>
    <sequence length="116" mass="13568" checksum="4DCED95DE14DFADD" modified="1996-11-01" version="1">MIEKVYEFKRDAKTKVVEKLVNTEHVQINHIVLPRGEQMPKHYSNSYVHLIIIKGEMTLTLEDQEPHNYKEGNIVYVPFNVKMLIQNINSDILEFFVVKAPHPKKLNAPEDPIKCE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="108" xmlns="http://uniprot.org/uniprot">
    <accession>Q58177</accession>
    <name>Y767_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized transcriptional regulator MJ0767</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0767</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64395">
        <property type="entry name" value="G64395"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870272.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58177"/>
    <dbReference type="SMR" id="Q58177"/>
    <dbReference type="STRING" id="243232.MJ_0767"/>
    <dbReference type="EnsemblBacteria" id="AAB98762">
        <property type="protein sequence ID" value="AAB98762"/>
        <property type="gene ID" value="MJ_0767"/>
    </dbReference>
    <dbReference type="GeneID" id="1451644"/>
    <dbReference type="KEGG" id="mja:MJ_0767"/>
    <dbReference type="eggNOG" id="arCOG01008">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_113319_1_2_2"/>
    <dbReference type="OMA" id="IVESKNY"/>
    <dbReference type="OrthoDB" id="90041at2157"/>
    <dbReference type="PhylomeDB" id="Q58177"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of transcription, DNA-templated"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="SSF47598"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="SSF55021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0238">DNA-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0805">Transcription regulation</keyword>
    <feature type="chain" id="PRO_0000139319" description="Uncharacterized transcriptional regulator MJ0767">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="135" mass="15570" checksum="4E921DFF975E031C" modified="1996-11-01" version="1">MVNVERISISFPKFLLKEIDEVVKKKGYSSRSELIRDAVRKYVLENNPLNKNETVSGIIIVVYNPTKEALEKMSKLYFEHNKVIKSLNQAYVTTSCGKNAKVEIFVVEGNSKDISKFYEEIEKINGKIYDKVIIF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58201</accession>
    <name>ARLY_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name type="ordered locus">MJ0791</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64398">
        <property type="entry name" value="G64398"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870298.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58201"/>
    <dbReference type="SMR" id="Q58201"/>
    <dbReference type="STRING" id="243232.MJ_0791"/>
    <dbReference type="EnsemblBacteria" id="AAB98785">
        <property type="protein sequence ID" value="AAB98785"/>
        <property type="gene ID" value="MJ_0791"/>
    </dbReference>
    <dbReference type="GeneID" id="1451670"/>
    <dbReference type="KEGG" id="mja:MJ_0791"/>
    <dbReference type="eggNOG" id="arCOG01748">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027272_2_3_2"/>
    <dbReference type="InParanoid" id="Q58201"/>
    <dbReference type="OMA" id="KKNPDVF"/>
    <dbReference type="OrthoDB" id="51806at2157"/>
    <dbReference type="PhylomeDB" id="Q58201"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="PTHR43814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="SSF48557"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000137862" description="Argininosuccinate lyase">
        <location>
            <begin position="1"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <sequence length="484" mass="55164" checksum="D99A3A370E981965" modified="1997-11-01" version="1">MNILRRGRLGNSIKEDVAKYTTSLSFDKEIFEADILCDIAHVIMLYEQGIIKKEDAKKIIEGLKEIYKKGMENLNLDPSLDDIHMVIESELIKKLGEDVAGRMHTGRSRNDEVATDLRIALREKVLIIAKSLIKMLKDILELAEKHKETLIVGYTHLQHAQPVTFAHHLLSYVSAIERDILRLLDAYKRINISPLGCGAMATTGFKINRERTKELLGFDALIENSMDGVSARDFILETMADLAILGTNLSKICEELILFSTYEFGTIEIANEFCSTSSIMPQKKNPDVAEIARAKLSKLNGNLVTALTILKALPNTYNRDLQEISPHLWDSVYTTIDTIKMVHGMLKTIKINKERMEELAKANYSTATELADTLVRETGIPFRTAHGIVGEVVRRSIEEKKDMIEVIYEVLEKYNLKVDEEKIKKALDPYENVKMRDVIGGPAPEEVEKRIKVFRERLDRYEKEVDEKLQKINKVKEILLSYEI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="103" xmlns="http://uniprot.org/uniprot">
    <accession>Q58208</accession>
    <name>Y798_METJA</name>
    <protein>
        <recommendedName>
            <fullName>TPR repeat-containing protein MJ0798</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0798</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64399">
        <property type="entry name" value="F64399"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870308.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58208"/>
    <dbReference type="SMR" id="Q58208"/>
    <dbReference type="STRING" id="243232.MJ_0798"/>
    <dbReference type="EnsemblBacteria" id="AAB98793">
        <property type="protein sequence ID" value="AAB98793"/>
        <property type="gene ID" value="MJ_0798"/>
    </dbReference>
    <dbReference type="GeneID" id="1451680"/>
    <dbReference type="KEGG" id="mja:MJ_0798"/>
    <dbReference type="eggNOG" id="arCOG03032">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_830580_0_0_2"/>
    <dbReference type="InParanoid" id="Q58208"/>
    <dbReference type="OMA" id="CYWNLVS"/>
    <dbReference type="OrthoDB" id="64283at2157"/>
    <dbReference type="PhylomeDB" id="Q58208"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001440">
        <property type="entry name" value="TPR_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_repeat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <keyword id="KW-0802">TPR repeat</keyword>
    <feature type="chain" id="PRO_0000106457" description="TPR repeat-containing protein MJ0798">
        <location>
            <begin position="1"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 1">
        <location>
            <begin position="102"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 2">
        <location>
            <begin position="137"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 3">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 4">
        <location>
            <begin position="204"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 5">
        <location>
            <begin position="236"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 6">
        <location>
            <begin position="273"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR 7">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <sequence length="334" mass="39695" checksum="1FC759BF15E64970" modified="1996-11-01" version="1">MAIKMDKNFTLEILNLLKNNVLKNRLKIGKELTEMLIDEIDKLIETANEISEEISKNSPNNSSLYDLRLIYNKLSTLYEDIDKLLGEIECILSLSNKDIKNWKLWKNLGDKAYLWKAYYEALFCYNKALELNQNTELLCKKGYALLKLYKRDLAIKYFEKASEKDRNNYKALFGLGKSYYLMSDNKNSIKYFEKVLELNPNDVEALEYLGELYYEEDCEKAINYFKKALELKPDDIDLILKVAFTYFKLKKYKHALKYFEKALKLNPNVFELEQIYESMGRIYIYLGEDEKAIECFEKLKEINLYHYEIYEIIALTYEEVGNIEKAKEFYKKLV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
    <accession>Q58245</accession>
    <name>Y835_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0835</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0835</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64404">
        <property type="entry name" value="C64404"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870347.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58245"/>
    <dbReference type="SMR" id="Q58245"/>
    <dbReference type="STRING" id="243232.MJ_0835"/>
    <dbReference type="EnsemblBacteria" id="AAB98838">
        <property type="protein sequence ID" value="AAB98838"/>
        <property type="gene ID" value="MJ_0835"/>
    </dbReference>
    <dbReference type="GeneID" id="1451719"/>
    <dbReference type="KEGG" id="mja:MJ_0835"/>
    <dbReference type="eggNOG" id="arCOG03421">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_732836_0_0_2"/>
    <dbReference type="OMA" id="FEVYNIY"/>
    <dbReference type="OrthoDB" id="34854at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007509">
        <property type="entry name" value="DUF515"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04415">
        <property type="entry name" value="DUF515"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107070" description="Uncharacterized protein MJ0835">
        <location>
            <begin position="1"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="377" mass="42951" checksum="FC2920714DE90FA9" modified="1996-11-01" version="1">MVDTSKIKALKEKSRRTVKSGSLKFILIILVVVIVGLLAFIAYNEISNLQFQEKITLENQKKAAIESINQMFAKYPNDPQKLIYINKIQMANNIEEINEVLEEAKKYISFKNYKIEAINQIKSMYGEYYSLSLSAQELVHKISLAQSTEEIENLLKSVDIEKDIRSIIEKQIDYVLASGDKYYYVEINGKSMFMTRDEILKYKKFWTLSELKSLKITPVSQLNKVAIEISAKQCGKLPHKGDIISIYSKDGSFITYGIIDSSYVILSSISYSESKSTSSNINELGESYSSSSSSSISYSLNNLPGILHATVIDRLDYDKIKKMFGEYGKKLNEIEDDTQIFDENVNYFLIISIPDDKIPDIIQIDPKDIVIVIKSKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="112" xmlns="http://uniprot.org/uniprot">
    <accession>Q58270</accession>
    <name>IDSA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Short chain isoprenyl diphosphate synthase</fullName>
            <ecNumber>2.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">idsA</name>
        <name type="ordered locus">MJ0860</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64407">
        <property type="entry name" value="D64407"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58270"/>
    <dbReference type="SMR" id="Q58270"/>
    <dbReference type="STRING" id="243232.MJ_0860"/>
    <dbReference type="EnsemblBacteria" id="AAB98865">
        <property type="protein sequence ID" value="AAB98865"/>
        <property type="gene ID" value="MJ_0860"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0860"/>
    <dbReference type="eggNOG" id="arCOG01726">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014015_2_1_2"/>
    <dbReference type="InParanoid" id="Q58270"/>
    <dbReference type="OMA" id="CEGQALD"/>
    <dbReference type="PhylomeDB" id="Q58270"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="SSF48576"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000123970" description="Short chain isoprenyl diphosphate synthase">
        <location>
            <begin position="1"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>isopentenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="241"/>
        </location>
        <ligand>
            <name>an all-trans-polyprenyl diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58914"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="P14324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000250" key="3">
        <source>
            <dbReference type="UniProtKB" id="Q12051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="327" mass="37569" checksum="07D68AC9BD657DAC" modified="1996-11-01" version="1">MKELFKRGGIMLFDKNILQKIDEELKTYVDKDDKLYNASKHLLFAGGKRIRPYLTVVTYMLKKDDIEEVLPAAAAVELIHNYTLIHDDIMDNDDERRGKPTVHVVYGEPMAILAGDLLYAKAFEAVSRIKDNKKAHEVLKILSKACVEVCEGQAMDMEFENYYPTMEEYLDMIRKKTGALLEASVGIGAVMADCNEEEREALKEYAKRIGLTFQIQDDVLDLIGDQKKLGKPVGSDIREGKKTIIVIHALKTLDEDKKKRLLEILGNKNVKDEEIKEAIEILKPSIEYAKELMKQKTEEAKEYLKIFNKDRRKVLEDLADFIMSRIY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="135" xmlns="http://uniprot.org/uniprot">
    <accession>Q58289</accession>
    <name>NIFH_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Nitrogenase iron protein</fullName>
            <ecNumber>1.18.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Nitrogenase Fe protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Nitrogenase component II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>Nitrogenase reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">nifH</name>
        <name type="ordered locus">MJ0879</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">The reversible ADP-ribosylation of Arg-97 inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64409">
        <property type="entry name" value="G64409"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870393.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58289"/>
    <dbReference type="SMR" id="Q58289"/>
    <dbReference type="STRING" id="243232.MJ_0879"/>
    <dbReference type="EnsemblBacteria" id="AAB98883">
        <property type="protein sequence ID" value="AAB98883"/>
        <property type="gene ID" value="MJ_0879"/>
    </dbReference>
    <dbReference type="GeneID" id="1451768"/>
    <dbReference type="KEGG" id="mja:MJ_0879"/>
    <dbReference type="eggNOG" id="arCOG00590">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059373_0_0_2"/>
    <dbReference type="InParanoid" id="Q58289"/>
    <dbReference type="OMA" id="EVDFHHE"/>
    <dbReference type="OrthoDB" id="66372at2157"/>
    <dbReference type="PhylomeDB" id="Q58289"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018697">
        <property type="term" value="F:carbonyl sulfide nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009399">
        <property type="term" value="P:nitrogen fixation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00533">
        <property type="entry name" value="NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005977">
        <property type="entry name" value="Nitrogenase_Fe_NifH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="PTHR42864"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01287">
        <property type="entry name" value="nifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0013">ADP-ribosylation</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0535">Nitrogen fixation</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0560">Oxidoreductase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000139539" description="Nitrogenase iron protein">
        <location>
            <begin position="1"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase" evidence="1">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="279" mass="30167" checksum="56DC15F981A20C54" modified="1996-11-01" version="1">MRKFCVYGKGGIGKSTTVSNIAAALAEDGKKVLVVGCDPKADTTRNLVGRKIPTVLDVFRKKGAENMKLEDIVFEGFGGVYCVESGGPEPGVGCAGRGVITAVDMLNRLGAFEELKPDVVIYDILGDVVCGGFAMPLQKHLADDVYIVTTCDPMAIYAANNICKGIKRYASRGKIALGGIIYNGRSVIDAPEIVKDFAKKIGTQVIGKIPMSNIITRAEIYKKTVIEYAPDSEIANTFREIAKAIYENENRVIPNPLSEEELDEITEKIDVLLKESVKG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="97" xmlns="http://uniprot.org/uniprot">
    <accession>Q58403</accession>
    <name>Y996_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Metalloprotease MJ0996</fullName>
            <ecNumber>3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0996</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probable metalloprotease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase U62 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64424">
        <property type="entry name" value="D64424"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870510.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58403"/>
    <dbReference type="SMR" id="Q58403"/>
    <dbReference type="STRING" id="243232.MJ_0996"/>
    <dbReference type="EnsemblBacteria" id="AAB99001">
        <property type="protein sequence ID" value="AAB99001"/>
        <property type="gene ID" value="MJ_0996"/>
    </dbReference>
    <dbReference type="GeneID" id="1451894"/>
    <dbReference type="KEGG" id="mja:MJ_0996"/>
    <dbReference type="eggNOG" id="arCOG00321">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026425_1_2_2"/>
    <dbReference type="InParanoid" id="Q58403"/>
    <dbReference type="OMA" id="TDFWGSM"/>
    <dbReference type="OrthoDB" id="22382at2157"/>
    <dbReference type="PhylomeDB" id="Q58403"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="SSF111283"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0482">Metalloprotease</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000142364" description="Metalloprotease MJ0996">
        <location>
            <begin position="1"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="451" mass="49814" checksum="D5245F2E9551FB79" modified="2000-05-30" version="2">MLNLEKIEKLLEVGDYADIRINFGESNTITLKDGKIEEISSGFGNGVAVRVLYKNGWGFVTSNIVSEEEIEKLINKAYKMAKISNEYSEKEIILKDYKAIIDNYKMIGKINPTDVDIEEKKEIIIDTYKNMTDEKIKSISVSYSDVFGKRIFMISEGSRIEGEITRCIMYMNCVAKENGNLQYGAERTGGFGFEKIKDNYLNLALEAKNRALRLLKAKPCPKGKFKVILDPELAGVFIHEAVGHASEADLVLQNDSVFKDKLGERVGSEYVTVIDDATIEGAFGSYKYDDEGVEGKKTVIIENGILKTYLHSRETAGRMDAELTGNGRAEGLNKPIVRMSNTFIKPGDWSFEELLEDTKEGIFLKGSRGGQVDTGKGLFQFSAVEAYLIENGELTQVLKDAGLSGEILDILFKVDAVTKDFELSVGYCGKDGQSVPVGDGGGCVRTIATVS</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="145" xmlns="http://uniprot.org/uniprot">
    <accession>Q58409</accession>
    <name>HACA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Methanogen homoaconitase large subunit</fullName>
            <shortName>HACN</shortName>
            <ecNumber evidence="3">4.2.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">hacA</name>
        <name type="ordered locus">MJ1003</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2007" name="J. Bacteriol." volume="189" first="4391" last="4400">
            <title>Enzymology and evolution of the pyruvate pathway to 2-oxobutyrate in Methanocaldococcus jannaschii.</title>
            <authorList>
                <person name="Drevland R.M."/>
                <person name="Waheed A."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="17449626"/>
            <dbReference type="DOI" id="10.1128/JB.00166-07"/>
        </citation>
        <scope>PUTATIVE FUNCTION AS A HOMOACONITASE</scope>
        <scope>LACK OF FUNCTION AS A ISOPROPYLMALATE ISOMERASE</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2008" name="J. Biol. Chem." volume="283" first="28888" last="28896">
            <title>Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.</title>
            <authorList>
                <person name="Drevland R.M."/>
                <person name="Jia Y."/>
                <person name="Palmer D.R.J."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="18765671"/>
            <dbReference type="DOI" id="10.1074/jbc.M802159200"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>COFACTOR</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>BIOPHYSICOCHEMICAL PROPERTIES</scope>
    </reference>
    <reference key="4">
        <citation type="journal article" date="2010" name="Biochemistry" volume="49" first="2687" last="2696">
            <title>Substrate specificity determinants of the methanogen homoaconitase enzyme: structure and function of the small subunit.</title>
            <authorList>
                <person name="Jeyakanthan J."/>
                <person name="Drevland R.M."/>
                <person name="Gayathri D.R."/>
                <person name="Velmurugan D."/>
                <person name="Shinkai A."/>
                <person name="Kuramitsu S."/>
                <person name="Yokoyama S."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="20170198"/>
            <dbReference type="DOI" id="10.1021/bi901766z"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>CATALYTIC ACTIVITY</scope>
        <scope>SUBSTRATE SPECIFICITY</scope>
        <scope>KINETIC PARAMETERS</scope>
    </reference>
    <comment type="function">
        <text evidence="3 4">Component of a hydro-lyase with broad substrate specificity for cis-unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4-tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)-aconitate, and even the non-physiological cis-homo(4)-aconitate with similar efficiency. These reactions are part of the biosynthesis pathway of coenzyme B. Can also catalyze the hydration of maleate to (R)-malate, and that of cis-aconitate. Cannot catalyze the hydration of citraconate and the dehydration of (S)-homocitrate, citramalate, 2-isopropylmalate, 3-isopropylmalate, citrate or threo-DL-isocitrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2R)-homocitrate = (2R,3S)-homoisocitrate</text>
            <dbReference type="Rhea" id="RHEA:32303"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:32304"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2R)-homocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:26101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:26102"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2R,3S)-homoisocitrate = cis-homoaconitate + H2O</text>
            <dbReference type="Rhea" id="RHEA:15485"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58174"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="5">
            <dbReference type="Rhea" id="RHEA:15487"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cis-(homo)2aconitate + H2O = (2R,3S)-iso(homo)2citrate</text>
            <dbReference type="Rhea" id="RHEA:68416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:72710"/>
            <dbReference type="ChEBI" id="CHEBI:72722"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:68417"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>cis-(homo)3aconitate + H2O = (2R,3S)-iso(homo)3citrate</text>
            <dbReference type="Rhea" id="RHEA:68420"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:72712"/>
            <dbReference type="ChEBI" id="CHEBI:177881"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:68421"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-malate = H2O + maleate</text>
            <dbReference type="Rhea" id="RHEA:23692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15588"/>
            <dbReference type="ChEBI" id="CHEBI:30780"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>cis-aconitate + H2O = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:22144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16383"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="1 3">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">22 uM for cis-homoaconitate (at 60 degrees Celsius)</KM>
            <KM evidence="3">30 uM for cis-homo(2)-aconitate (at 60 degrees Celsius)</KM>
            <KM evidence="3">36 uM for cis-homo(3)-aconitate (at 60 degrees Celsius)</KM>
            <KM evidence="3">175 uM for cis-homo(4)-aconitate (at 60 degrees Celsius)</KM>
            <KM evidence="3">330 uM for maleate (at 60 degrees Celsius)</KM>
            <KM evidence="3">1500 uM for (R)-homocitrate (at 60 degrees Celsius)</KM>
            <KM evidence="4">300 uM for cis-aconitate</KM>
            <Vmax evidence="3">0.68 umol/min/mg enzyme for cis-homoaconitate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="3">0.6 umol/min/mg enzyme for cis-homo(2)aconitate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="3">2.2 umol/min/mg enzyme for cis-homo(3)aconitate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="3">5.1 umol/min/mg enzyme for cis-homo(4)aconitate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="3">5.5 umol/min/mg enzyme for maleate hydration reaction (at 60 degrees Celsius)</Vmax>
            <Vmax evidence="3">0.59 umol/min/mg enzyme for (R)-homocitrate dehydration reaction (at 60 degrees Celsius)</Vmax>
            <text evidence="4">kcat is 0.75 sec(-1) for cis-aconitate hydration reaction (PubMed:20170198). Kinetic parameters measured using the HacAB complex.</text>
        </kinetics>
        <phDependence>
            <text evidence="3">Optimum pH is 9. Active from pH 8 to 10.</text>
        </phDependence>
    </comment>
    <comment type="pathway">
        <text evidence="5">Organic acid metabolism; 2-oxosuberate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of 2 HacA and 2 HacB proteins.</text>
    </comment>
    <comment type="miscellaneous">
        <text>The heterotetramer that can be formed in vitro between HacA and LeuD cannot catalyze citraconate hydration or the dehydration of 2-isopropylmalate or 3-isopropylmalate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.114" evidence="3"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64425">
        <property type="entry name" value="B64425"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870516.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="4KP2">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.50 A"/>
        <property type="chains" value="A/B=1-420"/>
    </dbReference>
    <dbReference type="PDBsum" id="4KP2"/>
    <dbReference type="AlphaFoldDB" id="Q58409"/>
    <dbReference type="SMR" id="Q58409"/>
    <dbReference type="STRING" id="243232.MJ_1003"/>
    <dbReference type="PRIDE" id="Q58409"/>
    <dbReference type="EnsemblBacteria" id="AAB99007">
        <property type="protein sequence ID" value="AAB99007"/>
        <property type="gene ID" value="MJ_1003"/>
    </dbReference>
    <dbReference type="GeneID" id="1451900"/>
    <dbReference type="KEGG" id="mja:MJ_1003"/>
    <dbReference type="eggNOG" id="arCOG01698">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_3_4_2"/>
    <dbReference type="InParanoid" id="Q58409"/>
    <dbReference type="OMA" id="GIEHCLL"/>
    <dbReference type="OrthoDB" id="15714at2157"/>
    <dbReference type="PhylomeDB" id="Q58409"/>
    <dbReference type="BioCyc" id="MetaCyc:MON-2003"/>
    <dbReference type="BRENDA" id="4.2.1.114">
        <property type="organism ID" value="3260"/>
    </dbReference>
    <dbReference type="SABIO-RK" id="Q58409"/>
    <dbReference type="UniPathway" id="UPA00919"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052633">
        <property type="term" value="F:isocitrate hydro-lyase (cis-aconitate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050075">
        <property type="term" value="F:maleate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="SSF53732"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000076868" description="Methanogen homoaconitase large subunit">
        <location>
            <begin position="1"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="3"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="21"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="27"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="turn" evidence="6">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="53"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="56"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="88"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="98"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="112"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="120"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="145"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="153"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="164"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="168"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="187"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="194"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="helix" evidence="6">
        <location>
            <begin position="201"/>
            <end position="209"/>
        </location>
    </feature>
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            <begin position="295"/>
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    <feature type="strand" evidence="6">
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    <feature type="strand" evidence="6">
        <location>
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        </location>
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    </feature>
    <feature type="strand" evidence="6">
        <location>
            <begin position="413"/>
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        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="17449626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="18765671"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="20170198"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PubMed" id="18765671"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="6">
        <source>
            <dbReference type="PDB" id="4KP2"/>
        </source>
    </evidence>
    <sequence length="420" mass="46063" checksum="ABDBAD2E101616B6" modified="1996-11-01" version="1">MTLVEKILSKKVGYEVCAGDSIEVEVDLAMTHDGTTPLAYKALKEMSDSVWNPDKIVVAFDHNVPPNTVKAAEMQKLALEFVKRFGIKNFHKGGEGICHQILAENYVLPNMFVAGGDSHTCTHGAFGAFATGFGATDMAYIYATGETWIKVPKTIRVDIVGKNENVSAKDIVLRVCKEIGRRGATYMAIEYGGEVVKNMDMDGRLTLCNMAIEMGGKTGVIEADEITYDYLKKERGLSDEDIAKLKKERITVNRDEANYYKEIEIDITDMEEQVAVPHHPDNVKPISDVEGTEINQVFIGSCTNGRLSDLREAAKYLKGREVHKDVKLIVIPASKKVFLQALKEGIIDIFVKAGAMICTPGCGPCLGAHQGVLAEGEICLSTTNRNFKGRMGHINSYIYLASPKIAAISAVKGYITNKLD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-08-03" version="123" xmlns="http://uniprot.org/uniprot">
    <accession>Q58456</accession>
    <name>ASNH2_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative asparagine synthetase [glutamine-hydrolyzing] 2</fullName>
            <ecNumber>6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1056</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the asparagine synthetase family.</text>
    </comment>
    <comment type="sequence caution" evidence="3">
        <conflict type="erroneous initiation">
            <sequence resource="EMBL-CDS" id="AAB99058" version="1"/>
        </conflict>
    </comment>
    <dbReference type="EC" id="6.3.5.4"/>
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        <property type="protein sequence ID" value="AAB99058.1"/>
        <property type="status" value="ALT_INIT"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="G64431"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58456"/>
    <dbReference type="SMR" id="Q58456"/>
    <dbReference type="STRING" id="243232.MJ_1056"/>
    <dbReference type="MEROPS" id="C44.001"/>
    <dbReference type="EnsemblBacteria" id="AAB99058">
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        <property type="gene ID" value="MJ_1056"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1056"/>
    <dbReference type="eggNOG" id="arCOG00071">
        <property type="taxonomic scope" value="Archaea"/>
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    <dbReference type="HOGENOM" id="CLU_014658_3_1_2"/>
    <dbReference type="InParanoid" id="Q58456"/>
    <dbReference type="OMA" id="IEHSHQP"/>
    <dbReference type="PhylomeDB" id="Q58456"/>
    <dbReference type="UniPathway" id="UPA00134">
        <property type="reaction ID" value="UER00195"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:L-asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_Synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SSF56235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000056938" description="Putative asparagine synthetase [glutamine-hydrolyzing] 2">
        <location>
            <begin position="2"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="52"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="378"/>
            <end position="379"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for beta-aspartyl-AMP intermediate formation" evidence="1">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="515" mass="60473" checksum="EE9EFBD4E643EDEB" modified="2003-09-26" version="3"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58480</accession>
    <name>Y1080_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1080</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1080</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64434">
        <property type="entry name" value="G64434"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58480"/>
    <dbReference type="STRING" id="243232.MJ_1080"/>
    <dbReference type="EnsemblBacteria" id="AAB99094">
        <property type="protein sequence ID" value="AAB99094"/>
        <property type="gene ID" value="MJ_1080"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1080"/>
    <dbReference type="eggNOG" id="arCOG00563">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1881087_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107161" description="Uncharacterized protein MJ1080">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="135" mass="15437" checksum="DDDFA77B995865FA" modified="1996-11-01" version="1">MEGKIMNIKHKIPILLLVLYIALGVFIQYNGISEFKSLPSPIYGGDYYYQMGVIWHIRDGGNPLESSSMIGGMPGYLPLYAYLCAKFCDLLNLDTMKGILYFSVVLFIMTSVIWFYLFRVLFKDDWVALIEVVLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-08-03" version="119" xmlns="http://uniprot.org/uniprot">
    <accession>Q58500</accession>
    <accession>B1A8K6</accession>
    <name>GLMM_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Phosphoglucosamine mutase</fullName>
            <ecNumber>5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">glmM</name>
        <name type="ordered locus">MJ1100</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2008" name="J. Bacteriol." volume="190" first="2987" last="2996">
            <title>Acetamido sugar biosynthesis in the Euryarchaea.</title>
            <authorList>
                <person name="Namboori S.C."/>
                <person name="Graham D.E."/>
            </authorList>
            <dbReference type="PubMed" id="18263721"/>
            <dbReference type="DOI" id="10.1128/JB.01970-07"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [GENOMIC DNA]</scope>
        <scope>SUBUNIT</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="1">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Forms large aggregates.</text>
    </comment>
    <comment type="PTM">
        <text evidence="1">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10"/>
    <dbReference type="EMBL" id="EU430076">
        <property type="protein sequence ID" value="ABZ91903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64437">
        <property type="entry name" value="C64437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870612.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58500"/>
    <dbReference type="SMR" id="Q58500"/>
    <dbReference type="STRING" id="243232.MJ_1100"/>
    <dbReference type="EnsemblBacteria" id="AAB99103">
        <property type="protein sequence ID" value="AAB99103"/>
        <property type="gene ID" value="MJ_1100"/>
    </dbReference>
    <dbReference type="GeneID" id="1451997"/>
    <dbReference type="KEGG" id="mja:MJ_1100"/>
    <dbReference type="eggNOG" id="arCOG00767">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_7_1_2"/>
    <dbReference type="InParanoid" id="Q58500"/>
    <dbReference type="OMA" id="PITCFKA"/>
    <dbReference type="OrthoDB" id="58941at2157"/>
    <dbReference type="PhylomeDB" id="Q58500"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_A">
        <property type="entry name" value="GlmM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023666">
        <property type="entry name" value="GlmM_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="SSF53738"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="SSF55957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0413">Isomerase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000148029" description="Phosphoglucosamine mutase">
        <location>
            <begin position="1"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="1">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="sequence conflict" description="In Ref. 1; ABZ91903." evidence="3" ref="1">
        <original>L</original>
        <variation>I</variation>
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="18263721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="448" mass="50099" checksum="FBC7EF17A73DF9B4" modified="1996-11-01" version="1">MGRLFGTSGIRMKNLSPKIAYKVGLAVAKKYKKVVVGRDTRTTGKLIETALTAGILNGGGEVTTINIVPTPVLGFNARNYDVGIMITASHNPPEYNGIKLFNKNGLAFNKKEEDEIEEIIFKEDFIEVEWHSVGEIWEDSRAIRNYMEHILKNVEINEKFNVVIDCANASACLVSPYLFTDLGCHVISVNSHMDGRFIGRLPEPDEKNLKKTMDMIKGLNMSGDNYIGIAHDGDADRMVAIDEKGRLADFDKLLAAFSRYMVEKTGNKKIVTTVDASMIIDEYLKDLDVEIIRTKVGDVAVAEEMIKNSAVFGGEPSGTWIHADIHLTPDGILSGLRVLEMLDFYNKKLYEILDEIPSYVNLREKIPCEDDKKEKVMSYVIENGESLFKTVPETVDGARFNLENGWVLIRPSGTEPYIRVRVEAKNNKDAKELLEKGIKLVKEALSAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-08-03" version="134" xmlns="http://uniprot.org/uniprot">
    <accession>Q58505</accession>
    <name>TRKA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Trk system potassium uptake protein TrkA homolog</fullName>
            <shortName>K(+)-uptake protein TrkA homolog</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">trkA</name>
        <name type="ordered locus">MJ1105</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2002" name="Cell" volume="109" first="781" last="791">
            <title>A mechanism of regulating transmembrane potassium flux through a ligand-mediated conformational switch.</title>
            <authorList>
                <person name="Roosild T.P."/>
                <person name="Miller S."/>
                <person name="Booth I.R."/>
                <person name="Choe S."/>
            </authorList>
            <dbReference type="PubMed" id="12086676"/>
            <dbReference type="DOI" id="10.1016/S0092-8674(02)00768-7"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1-136 IN COMPLEX WITH NAD</scope>
        <scope>DOMAIN</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer. Dimer of dimers.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The RCK N-terminal domain binds NAD and possibly other effectors. This is expected to cause a conformation change that regulates potassium transport.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64437">
        <property type="entry name" value="H64437"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870617.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="1LSS">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.30 A"/>
        <property type="chains" value="A/B/C/D=1-136"/>
    </dbReference>
    <dbReference type="PDBsum" id="1LSS"/>
    <dbReference type="AlphaFoldDB" id="Q58505"/>
    <dbReference type="SMR" id="Q58505"/>
    <dbReference type="STRING" id="243232.MJ_1105"/>
    <dbReference type="EnsemblBacteria" id="AAB99108">
        <property type="protein sequence ID" value="AAB99108"/>
        <property type="gene ID" value="MJ_1105"/>
    </dbReference>
    <dbReference type="GeneID" id="1452002"/>
    <dbReference type="KEGG" id="mja:MJ_1105"/>
    <dbReference type="eggNOG" id="arCOG01957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046525_2_3_2"/>
    <dbReference type="InParanoid" id="Q58505"/>
    <dbReference type="OMA" id="QRCNVVI"/>
    <dbReference type="OrthoDB" id="116057at2157"/>
    <dbReference type="PhylomeDB" id="Q58505"/>
    <dbReference type="EvolutionaryTrace" id="Q58505"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="SSF116726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="SSF51735"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0406">Ion transport</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0630">Potassium</keyword>
    <keyword id="KW-0633">Potassium transport</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000148725" description="Trk system potassium uptake protein TrkA homolog">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="3">
        <location>
            <begin position="136"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <begin position="7"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="4">
        <location>
            <begin position="73"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="2"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="25"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="44"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="55"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="turn" evidence="5">
        <location>
            <begin position="61"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="67"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="76"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="94"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="103"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="strand" evidence="5">
        <location>
            <begin position="113"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="helix" evidence="5">
        <location>
            <begin position="118"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="4">
        <source>
            <dbReference type="PubMed" id="12086676"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="5">
        <source>
            <dbReference type="PDB" id="1LSS"/>
        </source>
    </evidence>
    <sequence length="218" mass="23996" checksum="28CFD33E8791E0CC" modified="1996-11-01" version="1">MYIIIAGIGRVGYTLAKSLSEKGHDIVLIDIDKDICKKASAEIDALVINGDCTKIKTLEDAGIEDADMYIAVTGKEEVNLMSSLLAKSYGINKTIARISEIEYKDVFERLGVDVVVSPELIAANYIEKLIERPGILDLAIVGRGEAEILEFIIPEKAKVVNKKIKELGRPQDYLIIAIYDGDELKIPSGDTELKSGDRVLVLVKKDAADAIRKMFLEE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q58511</accession>
    <accession>Q58512</accession>
    <name>CCA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>CCA-adding enzyme</fullName>
            <ecNumber>2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName>tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">cca</name>
        <name type="ordered locus">MJ1111</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="submission" date="1998-02" db="EMBL/GenBank/DDBJ databases">
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Roberts K.M."/>
                <person name="Hurst M.A."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
        </citation>
        <scope>SEQUENCE REVISION</scope>
    </reference>
    <reference key="3">
        <citation type="journal article" date="2003" name="Proteins" volume="52" first="349" last="359">
            <title>Fold recognition, homology modeling, docking simulations, kinetics analysis and mutagenesis of ATP/CTP:tRNA nucleotidyltransferase from Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bujnicki J.M."/>
                <person name="Albert M.A."/>
                <person name="Nelson D.J."/>
                <person name="Thurlow D.L."/>
            </authorList>
            <dbReference type="PubMed" id="12866049"/>
            <dbReference type="DOI" id="10.1002/prot.10374"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>KINETIC PARAMETERS</scope>
        <scope>3D-STRUCTURE MODELING</scope>
        <scope>MUTAGENESIS OF ARG-164; LYS-167 AND LYS-171</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>a tRNA precursor + ATP + 2 CTP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="biophysicochemical properties">
        <kinetics>
            <KM evidence="3">21 uM for ATP</KM>
            <KM evidence="3">38 uM for CTP</KM>
        </kinetics>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58511"/>
    <dbReference type="SMR" id="Q58511"/>
    <dbReference type="STRING" id="243232.MJ_1111"/>
    <dbReference type="EnsemblBacteria" id="AAB99114">
        <property type="protein sequence ID" value="AAB99114"/>
        <property type="gene ID" value="MJ_1111"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1111"/>
    <dbReference type="eggNOG" id="arCOG04249">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044679_1_0_2"/>
    <dbReference type="InParanoid" id="Q58511"/>
    <dbReference type="OMA" id="DVDLVPC"/>
    <dbReference type="PhylomeDB" id="Q58511"/>
    <dbReference type="SABIO-RK" id="Q58511"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052929">
        <property type="term" value="F:ATP:3'-cytidine-cytidine-tRNA adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052928">
        <property type="term" value="F:CTP:3'-cytidine-tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052927">
        <property type="term" value="F:CTP:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016437">
        <property type="term" value="F:tRNA cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="PTHR39643"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="SSF55003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="SSF81301"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0692">RNA repair</keyword>
    <keyword id="KW-0694">RNA-binding</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000139069" description="CCA-adding enzyme">
        <location>
            <begin position="1"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="mutagenesis site" description="Loss of both AMP and CMP incorporation." evidence="3">
        <original>R</original>
        <variation>A</variation>
        <location>
            <position position="164"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="Loss of AMP incorporation and high decrease in CMP incorporation." evidence="3">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="mutagenesis site" description="No decrease in both AMP and CMP incorporation." evidence="3">
        <original>K</original>
        <variation>A</variation>
        <location>
            <position position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="3">
        <source>
            <dbReference type="PubMed" id="12866049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="449" mass="52784" checksum="F9BFACC8157AE994" modified="1998-06-01" version="2">MIVLTIEEILKEVLNEIKPSKEDMEKLQLKANEIIDKIWEIVRENSYPILEVLLVGSSARNTNLKDDYDIDIFVLFDKSVSEDELEEIGLKIGTEAIKRLNGSYNINYASHPYVNGEVDGYEVDIVPCYKIDFGEKIISAVDRTPLHHKFLISRLNERLCDEVRLLKAFLKSLGLYGSDVKTKGFSGYLCELLILHYGSFINLLKEAQNWRIGKKIILKDIFEIYKDVDINKLKKFDEPFIVYDPVDLNRNVASPLSKDNFCRFIFYSRQFLKNPSIEFFKDYAKKLEEILENREHGYRLILKIPRENVVDDIIYPQMEKLQKSINKVIVKNEFVILNSKCFADDNYCYLYWEFLVYELPKIALREGPPVFEKERAERFLKKYGKVFIRDCKLFAYTEREYSHIIDLFKDIVNGNLQNISIPKYVNPRNGKIIELNSHGEHKQFNKECQ</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-05-25" version="76" xmlns="http://uniprot.org/uniprot">
    <accession>Q58514</accession>
    <name>Y1114_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1114</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1114</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64439">
        <property type="entry name" value="A64439"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870625.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58514"/>
    <dbReference type="SMR" id="Q58514"/>
    <dbReference type="STRING" id="243232.MJ_1114"/>
    <dbReference type="EnsemblBacteria" id="AAB99122">
        <property type="protein sequence ID" value="AAB99122"/>
        <property type="gene ID" value="MJ_1114"/>
    </dbReference>
    <dbReference type="GeneID" id="1452010"/>
    <dbReference type="KEGG" id="mja:MJ_1114"/>
    <dbReference type="eggNOG" id="arCOG05068">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1431662_0_0_2"/>
    <dbReference type="OMA" id="HYRSGFR"/>
    <dbReference type="OrthoDB" id="103854at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107173" description="Uncharacterized protein MJ1114">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <sequence length="192" mass="21244" checksum="AA414E24D0042C2A" modified="1998-12-15" version="3">MDIVEKVYKEGILKLKENIPQIIINLVVAGLIWVFGILVFIPIADMLGNPYLFGLTALKPIISAIITIALIIVLLRVTKDFGELMDGIADIIAVKLAGSRVNEEKLKKYRRGLRGLAYLIVAIIAYLFFLPVISGITPVLAGIVLIILVLWAVTVLINIGHIFSEEIEEGIRIATEKLEKALEKSVKNEENE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-24" modified="2022-05-25" version="84" xmlns="http://uniprot.org/uniprot">
    <accession>Q58529</accession>
    <name>Y1129_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1129</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1129</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64440">
        <property type="entry name" value="H64440"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870640.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58529"/>
    <dbReference type="SMR" id="Q58529"/>
    <dbReference type="STRING" id="243232.MJ_1129"/>
    <dbReference type="EnsemblBacteria" id="AAB99131">
        <property type="protein sequence ID" value="AAB99131"/>
        <property type="gene ID" value="MJ_1129"/>
    </dbReference>
    <dbReference type="GeneID" id="1452025"/>
    <dbReference type="KEGG" id="mja:MJ_1129"/>
    <dbReference type="eggNOG" id="arCOG01845">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091588_3_0_2"/>
    <dbReference type="InParanoid" id="Q58529"/>
    <dbReference type="OMA" id="TVADPHM"/>
    <dbReference type="OrthoDB" id="113911at2157"/>
    <dbReference type="PhylomeDB" id="Q58529"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="SSF117916"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107181" description="Uncharacterized protein MJ1129">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <sequence length="95" mass="10580" checksum="99AAA7B868562AD0" modified="1996-11-01" version="1">MVTKEDVLNALKTVADPHMGISIVDMGLIRDVEVDDEGNVKFKLIPTNPYCMSVMAMAFQAKEAVKSLEGVKKVEVTVEGHVMEKDINEMLKEKE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2001-04-27" modified="2022-08-03" version="137" xmlns="http://uniprot.org/uniprot">
    <accession>Q58531</accession>
    <name>GUAAB_METJA</name>
    <protein>
        <recommendedName>
            <fullName>GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber>6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">guaAB</name>
        <name type="ordered locus">MJ1131</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>ATP + H2O + L-glutamine + XMP = AMP + diphosphate + GMP + 2 H(+) + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64441">
        <property type="entry name" value="B64441"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870642.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="6JP9">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.10 A"/>
        <property type="chains" value="A/B/C/D=1-310"/>
    </dbReference>
    <dbReference type="PDBsum" id="6JP9"/>
    <dbReference type="AlphaFoldDB" id="Q58531"/>
    <dbReference type="SMR" id="Q58531"/>
    <dbReference type="STRING" id="243232.MJ_1131"/>
    <dbReference type="EnsemblBacteria" id="AAB99133">
        <property type="protein sequence ID" value="AAB99133"/>
        <property type="gene ID" value="MJ_1131"/>
    </dbReference>
    <dbReference type="GeneID" id="1452027"/>
    <dbReference type="KEGG" id="mja:MJ_1131"/>
    <dbReference type="eggNOG" id="arCOG00085">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014340_0_0_2"/>
    <dbReference type="InParanoid" id="Q58531"/>
    <dbReference type="OMA" id="EGGIKSH"/>
    <dbReference type="OrthoDB" id="31932at2157"/>
    <dbReference type="PhylomeDB" id="Q58531"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016462">
        <property type="term" value="F:pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <ligand>
            <name>ATP</name>
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        <location>
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        </location>
    </feature>
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    </feature>
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            <dbReference type="PDB" id="6JP9"/>
        </source>
    </evidence>
    <sequence length="310" mass="34806" checksum="77F966F92FC62B12" modified="1996-11-01" version="1">MFDPKKFIDEAVEEIKQQISDRKAIIALSGGVDSSVAAVLTHKAIGDKLTAVFVDTGLMRKGEREEVEKTFRDKLGLNLIVVDAKDRFLNALKGVTDPEEKRKIIGKLFIDVFEEIAEDIKAEVLVQGTIAPDWIETQGKIKSHHNVALPHGMVLEVVEPLRELYKDEVRLLAKELGLPDSIVYRQPFPGPGLAVRVLGEVTEEKLNICREANAIVEEEVKKANLDKDLWQYFAVVLDCKATGVKGDEREYNWIVALRMVKSLDAMTAHVPEIPFDLLKRISKRITSEIPNVARVVFDITDKPPATIEFE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="114" xmlns="http://uniprot.org/uniprot">
    <accession>Q58564</accession>
    <name>Y1164_METJA</name>
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        <recommendedName>
            <fullName evidence="1">Putative HTH-type transcriptional regulatory protein MJ1164</fullName>
        </recommendedName>
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        <name type="ordered locus">MJ1164</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
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            <taxon>Methanocaldococcaceae</taxon>
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        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
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</entry>
<entry dataset="Swiss-Prot" created="2004-05-10" modified="2022-08-03" version="104" xmlns="http://uniprot.org/uniprot">
    <accession>Q58566</accession>
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    </protein>
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
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        <lineage>
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    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
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                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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        <text evidence="3">Binds 8 [4Fe-4S] clusters.</text>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0677">Repeat</keyword>
    <feature type="chain" id="PRO_0000159140" description="Polyferredoxin protein FwdF">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
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            <begin position="24"/>
            <end position="53"/>
        </location>
    </feature>
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        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
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        <location>
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            <end position="137"/>
        </location>
    </feature>
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        <location>
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            <end position="176"/>
        </location>
    </feature>
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        <location>
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            <end position="216"/>
        </location>
    </feature>
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        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
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        <location>
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            <end position="296"/>
        </location>
    </feature>
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        <location>
            <begin position="304"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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        <ligand>
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<entry dataset="Swiss-Prot" created="2000-05-30" modified="2022-05-25" version="91" xmlns="http://uniprot.org/uniprot">
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            <fullName>UPF0145 protein MJ1170</fullName>
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        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
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            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
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            <taxon>Methanocaldococcus</taxon>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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        <text evidence="1">Belongs to the UPF0145 family. Highly divergent.</text>
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</entry>
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        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
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                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
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                <person name="FitzGerald L.M."/>
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                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
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                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
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                <person name="Geoghagen N.S.M."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
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        </source>
    </evidence>
    <sequence length="296" mass="33205" checksum="7A4CDB4858A9719C" modified="1996-11-01" version="1">MELTVVQREILQELINLYREKNRPIKGTEIALRLNRNPGTIRNQMQALRALDLVDGVPGPKGGYVPTSKAYRALGLEDEGEIIVPIYKDGKKVEGVKVVKIEFDTVSHEKCCSSKIHIEGDTKHFNIGDIIRVGPTYHNKIIINGKIIGRDDIHRILLIDVLGVSSIPNIKVGDVGIKEVWTINPNCTLRETAKLFAEKYISGAPVVDNDKLVGVISLHDIAENIDNIDKKVKEVMRRDVITIHKDEKIYDALKIMNKNNVGRLVIVDDNNKIVGIITRTDILKIISGKFPENFHT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-07-11" modified="2022-08-03" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58658</accession>
    <name>NURA_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Probable DNA double-strand break repair nuclease NurA</fullName>
            <ecNumber evidence="1">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">nurA</name>
        <name evidence="3" type="ordered locus">MJ1262</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in DNA double-strand break (DSB) repair (By similarity). Acts probably with HerA to stimulate resection of the 5' strand and produce the long 3' single-strand that is required for RadA loading.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NurA family.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64457">
        <property type="entry name" value="E64457"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58658"/>
    <dbReference type="SMR" id="Q58658"/>
    <dbReference type="STRING" id="243232.MJ_1262"/>
    <dbReference type="EnsemblBacteria" id="AAB99265">
        <property type="protein sequence ID" value="AAB99265"/>
        <property type="gene ID" value="MJ_1262"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1262"/>
    <dbReference type="eggNOG" id="arCOG00367">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056881_0_0_2"/>
    <dbReference type="InParanoid" id="Q58658"/>
    <dbReference type="OMA" id="YILKKSH"/>
    <dbReference type="PhylomeDB" id="Q58658"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0227">DNA damage</keyword>
    <keyword id="KW-0234">DNA repair</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0540">Nuclease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107244" description="Probable DNA double-strand break repair nuclease NurA">
        <location>
            <begin position="1"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q8U1N8"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <evidence type="ECO:0000312" key="3">
        <source>
            <dbReference type="EMBL" id="AAB99265.1"/>
        </source>
    </evidence>
    <sequence length="389" mass="45314" checksum="28DBE8BEB9476404" modified="1996-11-01" version="1">MFFKIFVENTVILPNPLILTKIEGLLMIEYLLKNREQIIKKMEKINDINRKEVEEKWILDNFENPKDMGFAGGDGSCNKLDYISFSFYGVGAVSFIHGRGEKVKKAKEEYIFDITHPLDIEDRIRRYMLTLELKTALYVLKNYNIDYYIFDGSLFSLLIFTKKGIEMYERELEEIYNEYGKEFNKKIDEETKSGEIGIISKDLNLELNKKILVEHVEYILTLTKLINEFKDRIIGISKTSKINIYFDKNMPDIAIFTKYTDKSGYSEPIDFVNKLGDEKKEKHKQLSSVVKGINFIKNKPFYAKIDTAYIQFVRLEDNCGVVGLTSFNKIDKEVLSSLKEISINGYPYILKKSHETVEITTKKLEAIAKLLNIDDPIARHILGKKKKKF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58677</accession>
    <name>Y1281_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1281</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1281</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="H64459">
        <property type="entry name" value="H64459"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58677"/>
    <dbReference type="STRING" id="243232.MJ_1281"/>
    <dbReference type="EnsemblBacteria" id="AAB99287">
        <property type="protein sequence ID" value="AAB99287"/>
        <property type="gene ID" value="MJ_1281"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1281"/>
    <dbReference type="eggNOG" id="arCOG08205">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="eggNOG" id="arCOG09753">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_292822_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107249" description="Uncharacterized protein MJ1281">
        <location>
            <begin position="1"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="1048" mass="121015" checksum="14138CFDCE6A8A76" modified="1998-06-01" version="2"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58680</accession>
    <name>Y1284_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1284</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1284</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">To B.burgdorferi BB0465 N-terminal region.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64460">
        <property type="entry name" value="C64460"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870799.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58680"/>
    <dbReference type="STRING" id="243232.MJ_1284"/>
    <dbReference type="EnsemblBacteria" id="AAB99293">
        <property type="protein sequence ID" value="AAB99293"/>
        <property type="gene ID" value="MJ_1284"/>
    </dbReference>
    <dbReference type="GeneID" id="1452184"/>
    <dbReference type="KEGG" id="mja:MJ_1284"/>
    <dbReference type="eggNOG" id="arCOG08269">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2056048_0_0_2"/>
    <dbReference type="OMA" id="RLILWWR"/>
    <dbReference type="OrthoDB" id="105616at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000014011" description="Uncharacterized protein MJ1284">
        <location>
            <begin position="32"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="121" mass="14084" checksum="51E484F654BF6712" modified="1996-11-01" version="1" precursor="true">MILNNKGFIRILEATIAGIMVILVFSYLVMSQNFDYNLSLEFIGYNALYSAHIEEGDFENISSLYKKIELPSNVGYGFEIYKNGNLIYSDAKNGVVVERNFIFENNTSVNFYKLRLILWWR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-12-15" modified="2022-08-03" version="127" xmlns="http://uniprot.org/uniprot">
    <accession>Q58694</accession>
    <name>BIOF_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative 8-amino-7-oxononanoate synthase</fullName>
            <shortName>AONS</shortName>
            <ecNumber>2.3.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>7-keto-8-amino-pelargonic acid synthase</fullName>
            <shortName>7-KAP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName>8-amino-7-ketopelargonate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">bioF</name>
        <name type="ordered locus">MJ1298</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>6-carboxyhexanoyl-[ACP] + H(+) + L-alanine = (8S)-8-amino-7-oxononanoate + CO2 + holo-[ACP]</text>
            <dbReference type="Rhea" id="RHEA:42288"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78846"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="EC" id="2.3.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text>Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64462">
        <property type="entry name" value="A64462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870815.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58694"/>
    <dbReference type="SMR" id="Q58694"/>
    <dbReference type="STRING" id="243232.MJ_1298"/>
    <dbReference type="EnsemblBacteria" id="AAB99305">
        <property type="protein sequence ID" value="AAB99305"/>
        <property type="gene ID" value="MJ_1298"/>
    </dbReference>
    <dbReference type="GeneID" id="1452200"/>
    <dbReference type="KEGG" id="mja:MJ_1298"/>
    <dbReference type="eggNOG" id="arCOG00113">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_11_3_2"/>
    <dbReference type="InParanoid" id="Q58694"/>
    <dbReference type="OMA" id="MDTHGFG"/>
    <dbReference type="OrthoDB" id="39668at2157"/>
    <dbReference type="PhylomeDB" id="Q58694"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004723">
        <property type="entry name" value="AONS_Archaea/Proteobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="SSF53383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00858">
        <property type="entry name" value="bioF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0093">Biotin biosynthesis</keyword>
    <keyword id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000163820" description="Putative 8-amino-7-oxononanoate synthase">
        <location>
            <begin position="1"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="94"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="192"/>
            <end position="195"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="223"/>
            <end position="226"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="1">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="372" mass="41895" checksum="145B2FA8A5F8B9D5" modified="1996-11-01" version="1">MFREKLRREIEIIKNNGLYRFLRKKDDGVLDFSSNDYLCLSKHPEVIEAVKEGLKYGAGSTGSRLTSGNINHQRLEEKIAEFKETERTLVYSSGYATNVGVISALCKKGDLILSDKLNHASIIDGCKLSKADVLIYNHCDVEHLTNLIEENWGKYNNLFIVTDGVFSMDGDIAPLRDLKKIADEFNAILIIDDAHGTGVLGDGRGTLKHFNLKPSDNIVQIGTLSKAIGGLGGFVCGIEEVVEYLINTSRSFIFSTALPPHVVEGCIKAFEIIEKTDIVKKLQKNIKIANKVFKKYEFIKEDNLTPIYPFIFKEKTMEIAEHLIKNNIFCVGIRYPTVPKGLERIRVSINVGHEKEDFELLCERIKEVYSSD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-04" modified="2022-08-03" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58700</accession>
    <name>Y1304_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Putative toxin MJ1304</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1304</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Putative toxin component of a putative type VII toxin-antitoxin (TA) system. Its cognate antitoxin might be MJ1305.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64462">
        <property type="entry name" value="G64462"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870821.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58700"/>
    <dbReference type="SMR" id="Q58700"/>
    <dbReference type="STRING" id="243232.MJ_1304"/>
    <dbReference type="PRIDE" id="Q58700"/>
    <dbReference type="EnsemblBacteria" id="AAB99313">
        <property type="protein sequence ID" value="AAB99313"/>
        <property type="gene ID" value="MJ_1304"/>
    </dbReference>
    <dbReference type="GeneID" id="1452206"/>
    <dbReference type="KEGG" id="mja:MJ_1304"/>
    <dbReference type="eggNOG" id="arCOG01191">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_123170_2_2_2"/>
    <dbReference type="InParanoid" id="Q58700"/>
    <dbReference type="OMA" id="LWNPLEE"/>
    <dbReference type="OrthoDB" id="128863at2157"/>
    <dbReference type="PhylomeDB" id="Q58700"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007842">
        <property type="entry name" value="HEPN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05168">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00748">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50910">
        <property type="entry name" value="HEPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="chain" id="PRO_0000107262" description="Putative toxin MJ1304">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="HEPN" evidence="1">
        <location>
            <begin position="15"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="152" mass="18008" checksum="1B234DC43E5D3C84" modified="1996-11-01" version="1">MEDSGFNIKYAKLFIKRAEEDLEVAKVLLKTNHYPDSVYHSQQCVEKAVKAVLILNGIIFRRHVVSGVFRNVIYEMKIEDSWKEKLLNLIPKIESLEEHWVMPRYPEPYFGELWNPLEEYTKEDAEECLKDAENVLEVIKDFLKEKYGLKQI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58738</accession>
    <name>Y1342_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1342</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1342</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64467">
        <property type="entry name" value="E64467"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58738"/>
    <dbReference type="EnsemblBacteria" id="AAB99359">
        <property type="protein sequence ID" value="AAB99359"/>
        <property type="gene ID" value="MJ_1342"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1342"/>
    <dbReference type="eggNOG" id="arCOG07626">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_143938_0_0_2"/>
    <dbReference type="OMA" id="EIPGSAC"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107286" description="Uncharacterized protein MJ1342">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <sequence length="114" mass="13134" checksum="73DB2C0F38AA616A" modified="1996-11-01" version="1">MLWVLGGKKIAKCLKETVNPKEIEDKELIKFLINFCDMCNDFVIDDEKIGIKIDKCKYCPKQIGEAEIPGSACPIPSILASCMRELTKKDYKINLWDGNKVIIKENGYCWFRIK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1999-07-15" modified="2022-08-03" version="129" xmlns="http://uniprot.org/uniprot">
    <accession>Q58762</accession>
    <name>WTPC_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="2">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">wtpC</name>
        <name type="ordered locus">MJ1367</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>ATP + H2O + tungstate(in) = ADP + H(+) + phosphate + tungstate(out)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="2"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64470">
        <property type="entry name" value="F64470"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870884.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58762"/>
    <dbReference type="SMR" id="Q58762"/>
    <dbReference type="STRING" id="243232.MJ_1367"/>
    <dbReference type="TCDB" id="3.A.1.6.9">
        <property type="family name" value="the atp-binding cassette (abc) superfamily"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99375">
        <property type="protein sequence ID" value="AAB99375"/>
        <property type="gene ID" value="MJ_1367"/>
    </dbReference>
    <dbReference type="GeneID" id="1452270"/>
    <dbReference type="KEGG" id="mja:MJ_1367"/>
    <dbReference type="eggNOG" id="arCOG00175">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_1_1_2"/>
    <dbReference type="InParanoid" id="Q58762"/>
    <dbReference type="OMA" id="HFIILGP"/>
    <dbReference type="OrthoDB" id="88924at2157"/>
    <dbReference type="PhylomeDB" id="Q58762"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0500">Molybdenum</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-1278">Translocase</keyword>
    <keyword id="KW-0813">Transport</keyword>
    <feature type="chain" id="PRO_0000093225" description="Molybdate/tungstate import ATP-binding protein WtpC">
        <location>
            <begin position="1"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="2"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="32"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000250" key="2">
        <source>
            <dbReference type="UniProtKB" id="Q8U4K3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="297" mass="33266" checksum="710DB22733EEB122" modified="1996-11-01" version="1">MLKVNNLSKIWKDFKLKNVSFEIDREYCVILGPSGAGKSVLIKCIAGILKPDSGRIILNGEDITNLPPEKRNVGYVPQNYALFPNKNVYKNIAYGLIIKKVNKLEIDRKVKEIAEFLNISHLLNRDVKTLSGGEQQRVALARALILNPSILLLDEPTSAVDIKIKESIISELKKIKHIPVLHITHDLAEARTLGEKVGIFMNGELIAFGDKSILKKPKNKKVAEFLGFNIIDDKAIAPEDVIIKDGNGGEVVNIIDYGKYKKVFVKYNGYIIKAFTERDLNIGDNVGLEFREQTKLT</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-10" modified="2022-05-25" version="88" xmlns="http://uniprot.org/uniprot">
    <accession>Q58765</accession>
    <name>Y1370_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0285 protein MJ1370</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1370</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0285 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64471">
        <property type="entry name" value="A64471"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870887.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58765"/>
    <dbReference type="SMR" id="Q58765"/>
    <dbReference type="STRING" id="243232.MJ_1370"/>
    <dbReference type="EnsemblBacteria" id="AAB99387">
        <property type="protein sequence ID" value="AAB99387"/>
        <property type="gene ID" value="MJ_1370"/>
    </dbReference>
    <dbReference type="GeneID" id="1452273"/>
    <dbReference type="KEGG" id="mja:MJ_1370"/>
    <dbReference type="eggNOG" id="arCOG04885">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_846254_0_0_2"/>
    <dbReference type="InParanoid" id="Q58765"/>
    <dbReference type="OMA" id="CYSMGDG"/>
    <dbReference type="OrthoDB" id="33713at2157"/>
    <dbReference type="PhylomeDB" id="Q58765"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01087">
        <property type="entry name" value="UPF0285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016735">
        <property type="entry name" value="UPF0285_methan"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018783">
        <property type="entry name" value="UCP018783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="SSF53067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR03281">
        <property type="entry name" value="methan_mark_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000151262" description="UPF0285 protein MJ1370">
        <location>
            <begin position="1"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01087"/>
        </source>
    </evidence>
    <sequence length="328" mass="35980" checksum="AC96B0820123FACF" modified="1996-11-01" version="1">MITVGIDHGTSGITTCIKDNDKKIIFKLKRTELKEKSYLEELEKHISLEDIDLIALTYSMGDGINKILPIEKVKNRGVLSIEGAGEKVGGGTKVYDEIKESGLPAVVIPGLHRGIECLDERFRALYSHIASPEKVSIAYYAYKLFGFNDFVLSDISSNTVTLLIKDGKIFGGFDACIGAIGMLHGPIDLEMIRDIDAGKITANEAFSKAGAVKIAKLYKGVENTKEEIINNYFNDENCRLAVDSLILSVSMEINSLLPLLDKNKRRVVLAGSIGTLRNPIDIPKRIKEFVEAKIFVLYGESGAIGGALIAEDILKGKRDILGIEVEFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-08-02" modified="2022-08-03" version="143" xmlns="http://uniprot.org/uniprot">
    <accession>Q58773</accession>
    <name>CARB1_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Carbamoyl-phosphate synthase large chain, N-terminal section</fullName>
            <ecNumber>6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Carbamoyl-phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">carB1</name>
        <name type="ordered locus">MJ1378</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction>
            <text>2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H(+) + L-glutamate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 2 magnesium or manganese ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text>Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text>Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text>Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.</text>
    </comment>
    <comment type="domain">
        <text>Corresponds to the N-terminal section.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">CarB is split into two genes in M.jannaschii (MJ1378 and MJ1381).</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870895.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58773"/>
    <dbReference type="SMR" id="Q58773"/>
    <dbReference type="STRING" id="243232.MJ_1378"/>
    <dbReference type="EnsemblBacteria" id="AAB99385">
        <property type="protein sequence ID" value="AAB99385"/>
        <property type="gene ID" value="MJ_1378"/>
    </dbReference>
    <dbReference type="GeneID" id="1452281"/>
    <dbReference type="KEGG" id="mja:MJ_1378"/>
    <dbReference type="eggNOG" id="arCOG01594">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000513_1_1_2"/>
    <dbReference type="InParanoid" id="Q58773"/>
    <dbReference type="OMA" id="ADRMFYL"/>
    <dbReference type="OrthoDB" id="10818at2157"/>
    <dbReference type="PhylomeDB" id="Q58773"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0006807">
        <property type="term" value="P:nitrogen compound metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo"/>
    </dbReference>
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        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
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        <property type="entry name" value="CbamoylP_synth_lsu-like_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="CbamoylP_synth_lsu_CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="SSF48108"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="SSF52440"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword id="KW-0055">Arginine biosynthesis</keyword>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000145075" description="Carbamoyl-phosphate synthase large chain, N-terminal section">
        <location>
            <begin position="1"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="2">
        <location>
            <begin position="130"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Carboxyphosphate synthetic domain">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="156"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="297"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="482" mass="53609" checksum="A8ABE2570B2E3D96" modified="1998-06-01" version="2">MESIKKVMVFGSGPIVIGQAAEFDFSGSQACKALKEEGIYTILVNSNPATIQTDTDMADKVYLEPLHPTIVEKIIEKERPDAILPTMGGQTGLNLALELHRRGILDKYGIKLLGSNIRTIEIAEDRELFAEAMAEINEPVTKCKAVNSVDEAVEFAEEIGYPVIVRPAFTLGGTGGGIAHNKEELIDITSKGLKYSIINQVLIDESVLGWKEFELEVMRDRKDTCIIVCGMENIDPMGIHTGESIVVSPIQTLPDEFYQKLRNAAIKIIRHLGIEGGCNIQFAVNKEMTEYRVIEVNPRVSRSSALASKATGYPIARIAAKIAIGKTLDEILNDVTKETPASFEPTLDYVVVKIPRWPFDKFKTVDKKLGTSMKSTGEVMAIGRSFEEALQKAIRSLDIGRFGIIGDGKDKDYTDEEIEEILKNPTDERIFVIAKALEKGWSVEKIVELTDIDEFFIKKIKNIVDMKKELEKLKEEIKKLNA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="80" xmlns="http://uniprot.org/uniprot">
    <accession>Q58784</accession>
    <name>Y1389_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1389</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1389</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99400.1"/>
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    </dbReference>
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        <property type="entry name" value="D64473"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870906.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58784"/>
    <dbReference type="SMR" id="Q58784"/>
    <dbReference type="STRING" id="243232.MJ_1389"/>
    <dbReference type="EnsemblBacteria" id="AAB99400">
        <property type="protein sequence ID" value="AAB99400"/>
        <property type="gene ID" value="MJ_1389"/>
    </dbReference>
    <dbReference type="GeneID" id="1452292"/>
    <dbReference type="KEGG" id="mja:MJ_1389"/>
    <dbReference type="eggNOG" id="arCOG02603">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912376_0_0_2"/>
    <dbReference type="OMA" id="IISRHMA"/>
    <dbReference type="OrthoDB" id="135513at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107306" description="Uncharacterized protein MJ1389">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <sequence length="134" mass="15253" checksum="0620AE6F7AA49E35" modified="1996-11-01" version="1">MKATENRKVNEINEILLPLSKNLKNVEGFVIVSKDSLVKVGNIDGEDLEIISRHMAVVMGSSEMLYKRFNDEVEYIEIKGKKHKIILYNLDDFIFAVVGNIKADEIKDKVMELKFKVNNIDGLTAENIIEEIAL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-08-22" modified="2022-05-25" version="83" xmlns="http://uniprot.org/uniprot">
    <accession>Q58790</accession>
    <name>Y1395_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1395</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1395</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
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            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="AAB99405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64474">
        <property type="entry name" value="B64474"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58790"/>
    <dbReference type="STRING" id="243232.MJ_1395"/>
    <dbReference type="EnsemblBacteria" id="AAB99405">
        <property type="protein sequence ID" value="AAB99405"/>
        <property type="gene ID" value="MJ_1395"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1395"/>
    <dbReference type="eggNOG" id="arCOG07532">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1912353_0_0_2"/>
    <dbReference type="PhylomeDB" id="Q58790"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107310" description="Uncharacterized protein MJ1395">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="132" mass="15215" checksum="FB202241F54A7318" modified="1996-11-01" version="1">MDVNVSYKDTYSVITYQVWEPIDGGKNITLIIEYDADIVDNGILFKTVSIPIGGDLNIKNFHINFVSPYYLTYQEPDGNNFQIPKKTLLIINAEFSILPLPKLPVHGYVVFWLSILCILIIIFVYTELRRKK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-05-25" version="74" xmlns="http://uniprot.org/uniprot">
    <accession>Q58809</accession>
    <name>Y1414_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1414</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1414</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64476">
        <property type="entry name" value="E64476"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870931.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58809"/>
    <dbReference type="STRING" id="243232.MJ_1414"/>
    <dbReference type="EnsemblBacteria" id="AAB99424">
        <property type="protein sequence ID" value="AAB99424"/>
        <property type="gene ID" value="MJ_1414"/>
    </dbReference>
    <dbReference type="GeneID" id="1452317"/>
    <dbReference type="KEGG" id="mja:MJ_1414"/>
    <dbReference type="eggNOG" id="arCOG07627">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1145239_0_0_2"/>
    <dbReference type="InParanoid" id="Q58809"/>
    <dbReference type="OMA" id="INTFSYN"/>
    <dbReference type="OrthoDB" id="134050at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107315" description="Uncharacterized protein MJ1414">
        <location>
            <begin position="1"/>
            <end position="241"/>
        </location>
    </feature>
    <sequence length="241" mass="27861" checksum="27936638E39481E2" modified="1996-11-01" version="1">MSPMRWAIFLVLLTITFSGCLNKEISKEEIIKKIDEINTFSYNAKVFINLSVSNPAINKVNMKMDIDGYSDGKLSKGIIHVYYTVRYFNGRNETIPFYVNEEGTFIKLEGKWQKITNNDLSNHTWNILAYIKDLIEKNDIKIEEENNHYIIRLKDENAEKQLNPFFYRGIKIPGINLKISEEEVVIILDKYGTPIKVIKKGKLYGTSSKGNLDGVIVIETEIKDINKDFDFSIPEDLSIYN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-08-03" version="106" xmlns="http://uniprot.org/uniprot">
    <accession>Q58832</accession>
    <name>G3PP_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glyceraldehyde 3-phosphate phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1437</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2015" name="Proc. Natl. Acad. Sci. U.S.A." volume="112" first="E1974" last="E1983">
            <title>Panoramic view of a superfamily of phosphatases through substrate profiling.</title>
            <authorList>
                <person name="Huang H."/>
                <person name="Pandya C."/>
                <person name="Liu C."/>
                <person name="Al-Obaidi N.F."/>
                <person name="Wang M."/>
                <person name="Zheng L."/>
                <person name="Toews Keating S."/>
                <person name="Aono M."/>
                <person name="Love J.D."/>
                <person name="Evans B."/>
                <person name="Seidel R.D."/>
                <person name="Hillerich B.S."/>
                <person name="Garforth S.J."/>
                <person name="Almo S.C."/>
                <person name="Mariano P.S."/>
                <person name="Dunaway-Mariano D."/>
                <person name="Allen K.N."/>
                <person name="Farelli J.D."/>
            </authorList>
            <dbReference type="PubMed" id="25848029"/>
            <dbReference type="DOI" id="10.1073/pnas.1423570112"/>
        </citation>
        <scope>FUNCTION</scope>
        <scope>COFACTOR</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dephosphorylation of D,L-glyceraldehyde 3-phosphate in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="4"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64479">
        <property type="entry name" value="D64479"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870955.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58832"/>
    <dbReference type="SMR" id="Q58832"/>
    <dbReference type="STRING" id="243232.MJ_1437"/>
    <dbReference type="DNASU" id="1452341"/>
    <dbReference type="EnsemblBacteria" id="AAB99446">
        <property type="protein sequence ID" value="AAB99446"/>
        <property type="gene ID" value="MJ_1437"/>
    </dbReference>
    <dbReference type="GeneID" id="1452341"/>
    <dbReference type="KEGG" id="mja:MJ_1437"/>
    <dbReference type="eggNOG" id="arCOG02291">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_3_2"/>
    <dbReference type="InParanoid" id="Q58832"/>
    <dbReference type="OMA" id="TYHNVKF"/>
    <dbReference type="OrthoDB" id="93004at2157"/>
    <dbReference type="PhylomeDB" id="Q58832"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050124">
        <property type="term" value="F:N-acylneuraminate-9-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016311">
        <property type="term" value="P:dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046380">
        <property type="term" value="P:N-acetylneuraminate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="SSF56784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107329" description="Glyceraldehyde 3-phosphate phosphatase">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000269" key="1">
        <source>
            <dbReference type="PubMed" id="25848029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="2">
        <source>
            <dbReference type="PubMed" id="25848029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="25848029"/>
        </source>
    </evidence>
    <sequence length="228" mass="26064" checksum="C832F9F435ABC3FF" modified="1996-11-01" version="1">MIKGILFDLDDTLYNSSEFVEIARREAVKSMIDAGLNIDFEEAMNILNKIIKDKGSNYGKHFDDLVKAVLGKYDPKIITTGIITYHNVKVALLRPYPHTIKTLMELKAMGLKLGVITDGLTIKQWEKLIRLGIHPFFDDVITSEEFGLGKPHLEFFKYGLKRMGLKAEETVYVGDRVDKDIKPAKELGMITVRILKGKYKDMEDDEYSDYTINSLQELVDIVKNLKKD</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-01-16" modified="2022-08-03" version="93" xmlns="http://uniprot.org/uniprot">
    <accession>Q58871</accession>
    <name>TRMJ_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (cytidine-2'-O-)-methyltransferase TrmJ</fullName>
            <ecNumber evidence="1">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">tRNA (cytidine(32)-2'-O)-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">tRNA Cm32 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">trmJ</name>
        <name type="ordered locus">MJ1476</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the formation of 2'O-methylated cytidine (Cm32) at position 32 in tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>cytidine(32) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(32) in tRNA + H(+) + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:42932"/>
            <dbReference type="Rhea" id="RHEA-COMP:10288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10289"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64484">
        <property type="entry name" value="C64484"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58871"/>
    <dbReference type="SMR" id="Q58871"/>
    <dbReference type="STRING" id="243232.MJ_1476"/>
    <dbReference type="EnsemblBacteria" id="AAB99481">
        <property type="protein sequence ID" value="AAB99481"/>
        <property type="gene ID" value="MJ_1476"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1476"/>
    <dbReference type="eggNOG" id="arCOG01018">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056931_3_0_2"/>
    <dbReference type="InParanoid" id="Q58871"/>
    <dbReference type="OMA" id="PIMNLSH"/>
    <dbReference type="PhylomeDB" id="Q58871"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052666">
        <property type="term" value="F:tRNA (cytosine-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052665">
        <property type="term" value="F:tRNA (uracil-2'-O-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004384">
        <property type="entry name" value="RNA_MeTrfase_TrmJ/LasT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001537">
        <property type="entry name" value="SpoU_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42786">
        <property type="entry name" value="PTHR42786"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00588">
        <property type="entry name" value="SpoU_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004808">
        <property type="entry name" value="LasT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="SSF75217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00050">
        <property type="entry name" value="rRNA_methyl_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0489">Methyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <keyword id="KW-0819">tRNA processing</keyword>
    <feature type="chain" id="PRO_0000159843" description="tRNA (cytidine-2'-O-)-methyltransferase TrmJ">
        <location>
            <begin position="1"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="79"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000250" key="1">
        <source>
            <dbReference type="UniProtKB" id="Q4JB16"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="230" mass="26280" checksum="E9B6B9B31B3A146E" modified="1996-11-01" version="1">MIIMISVILVNPKYSGNVGSIARVMMNFGFEELRIVGDKSIINNEAYMMAVHAREILDNAKFYNTFDEAIGDLDFVIATSGARGGDRNLKRVPITPKELADKILEVKGNIGIVFGREDDGLRNEEIDKCDLLVSIPTSEKYPIMNLSHAVAVILYEIYTKKVRNKFLDINMREASKEDKELLIRKFNEFIDKNEKIPEHKKELCKIIFKRLVNRAFISGKEAWTLMSAFK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2000-12-01" modified="2022-08-03" version="117" xmlns="http://uniprot.org/uniprot">
    <accession>Q58872</accession>
    <name>Y1477_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1477</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1477</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="D64484">
        <property type="entry name" value="D64484"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010870998.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58872"/>
    <dbReference type="STRING" id="243232.MJ_1477"/>
    <dbReference type="EnsemblBacteria" id="AAB99489">
        <property type="protein sequence ID" value="AAB99489"/>
        <property type="gene ID" value="MJ_1477"/>
    </dbReference>
    <dbReference type="GeneID" id="1452382"/>
    <dbReference type="KEGG" id="mja:MJ_1477"/>
    <dbReference type="eggNOG" id="arCOG07957">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058176_0_0_2"/>
    <dbReference type="InParanoid" id="Q58872"/>
    <dbReference type="OMA" id="DYRFYWK"/>
    <dbReference type="OrthoDB" id="46164at2157"/>
    <dbReference type="PhylomeDB" id="Q58872"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016063">
        <property type="entry name" value="Extracellular_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004352">
        <property type="entry name" value="GH114_TIM-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="Glycoside_hydrolase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016062">
        <property type="entry name" value="TM1410-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35882:SF1">
        <property type="entry name" value="PTHR35882:SF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03537">
        <property type="entry name" value="Glyco_hydro_114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01545">
        <property type="entry name" value="THEMAYE10DUF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="SSF51445"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01370">
        <property type="entry name" value="TIGR01370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000107359" description="Uncharacterized protein MJ1477">
        <location>
            <begin position="1"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="346" mass="40620" checksum="A71B3D69A956BFCE" modified="1996-11-01" version="1">MHLNKIRGKNMKKSHILGIIICIILIVGFFISFDSTFLDNPKMMSKSKNNIRNAENLTNISKNSNNLKFLWAYQLQNADIDEIANSNFTLIVIDYSKDGTENGKYSEEEIEKLKKAGKIPIAYISIGEAEDYRFYWDNEWLKNPPKWLGDENPEWEGCYAVKYWHPEWKKIIFSYLDKIIQQGFCGVYLDKVDEFEYWAENGYDEDFTAKEMIKFIVEISNYCRNKTNNSFIIIPQNGERLLEYDKHGKLLNTVSGWAVEDLFYDGVEQKTEEEINERIKLLDKVKDSGKFVLVVDYVDDGTKTNENLKRVEDFINKSLDKGYVPYVAKSDRELDELNTWWLKLIN</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-11-07" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q58875</accession>
    <name>Y1480_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1480</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1480</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64484">
        <property type="entry name" value="G64484"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871002.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="PDB" id="3MGJ">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="2.70 A"/>
        <property type="chains" value="A/B=1-110"/>
    </dbReference>
    <dbReference type="PDBsum" id="3MGJ"/>
    <dbReference type="AlphaFoldDB" id="Q58875"/>
    <dbReference type="SMR" id="Q58875"/>
    <dbReference type="STRING" id="243232.MJ_1480"/>
    <dbReference type="DNASU" id="1452386"/>
    <dbReference type="EnsemblBacteria" id="AAB99492">
        <property type="protein sequence ID" value="AAB99492"/>
        <property type="gene ID" value="MJ_1480"/>
    </dbReference>
    <dbReference type="GeneID" id="1452386"/>
    <dbReference type="KEGG" id="mja:MJ_1480"/>
    <dbReference type="eggNOG" id="arCOG04422">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056125_0_0_2"/>
    <dbReference type="InParanoid" id="Q58875"/>
    <dbReference type="OMA" id="GHKHHIY"/>
    <dbReference type="OrthoDB" id="22696at2157"/>
    <dbReference type="PhylomeDB" id="Q58875"/>
    <dbReference type="EvolutionaryTrace" id="Q58875"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="CDD" id="cd12144">
        <property type="entry name" value="SDH_N_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005239">
        <property type="entry name" value="CHP00300"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007545">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04455">
        <property type="entry name" value="Saccharop_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="SSF52467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00300">
        <property type="entry name" value="TIGR00300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107363" description="Uncharacterized protein MJ1480">
        <location>
            <begin position="1"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="2"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="31"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="helix" evidence="1">
        <location>
            <begin position="58"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="78"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="strand" evidence="1">
        <location>
            <begin position="86"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0007829" key="1">
        <source>
            <dbReference type="PDB" id="3MGJ"/>
        </source>
    </evidence>
    <sequence length="423" mass="47181" checksum="E2EFD16142F2816E" modified="1996-11-01" version="1">MFMREIELRGHIIDSLILPKVFDKILDMGGDYKVLEFEIGKRKTDPSYAKILVIGRDERHVDEILNELRDLGAEIPEIEEVELQPAEKDMVLPEGFYSTTNHKTFIRFKGKWIEVENQKMDGAIVVYPDEMRAEVKTIRNIKKGDLVVVGHKGVRVIPPEKPREGGGLFEFMKSDASSEKPKETIIRRIAKEMYEIREKYRKTGKGGIVVVGGPAIIHTGAGWALAKLIRMGYVQALFAGNALATHDIESVLYGTSLGVDLKTGKSVPGGHSHHLRAINTIMRAGSIKDAVEQGILKEGVMYECIKNNIPYVLAGSIRDDGPLPDVITDVVKAQEKMRELLKGKDMVLMLSTMLHSIATGNLLPSWVKTICVDINPAVVTKLMDRGTSQALGIVTDVGVFLPMLVEELEKLEKEKEKSEKREE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1998-07-15" modified="2022-05-25" version="72" xmlns="http://uniprot.org/uniprot">
    <accession>Q58962</accession>
    <name>Y1567_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1567</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1567</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64495">
        <property type="entry name" value="F64495"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q58962"/>
    <dbReference type="STRING" id="243232.MJ_1567"/>
    <dbReference type="EnsemblBacteria" id="AAB99593">
        <property type="protein sequence ID" value="AAB99593"/>
        <property type="gene ID" value="MJ_1567"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1567"/>
    <dbReference type="eggNOG" id="arCOG08282">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1582910_0_0_2"/>
    <dbReference type="InParanoid" id="Q58962"/>
    <dbReference type="OMA" id="KFINISY"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107415" description="Uncharacterized protein MJ1567">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <sequence length="175" mass="20726" checksum="20524D646C38EFD6" modified="1996-11-01" version="1">MVAMNENQQKLHYIINLLTNGNKKRWVKQTVLFALIYYFIKLDVFKGYDYAPTPFMWEDEIKFINISYEAINDLNYLLDNNYLNEILLSVRGLNEFIVGYSIGKKIDYNFNQKDKETIDKTLLENGRLKEIQITKNGIIIKSKNEKLEIEITKIDKISYKSKSYIMKVSLWDSNI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q59001</accession>
    <name>GLGA_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Probable glycogen synthase</fullName>
            <ecNumber>2.4.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName>Starch [bacterial glycogen] synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="primary">glgA</name>
        <name type="ordered locus">MJ1606</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Synthesizes alpha-1,4-glucan chains using ADP-glucose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + ADP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18189"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:57498"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.4.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text>Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 1 family. Bacterial/plant glycogen synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">Could lack activity as the potential ADP-glucose binding site Arg/Lys residue in position 15 is replaced by an Ser.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.21"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="E64500">
        <property type="entry name" value="E64500"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871131.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59001"/>
    <dbReference type="SMR" id="Q59001"/>
    <dbReference type="STRING" id="243232.MJ_1606"/>
    <dbReference type="CAZy" id="GT5">
        <property type="family name" value="Glycosyltransferase Family 5"/>
    </dbReference>
    <dbReference type="EnsemblBacteria" id="AAB99625">
        <property type="protein sequence ID" value="AAB99625"/>
        <property type="gene ID" value="MJ_1606"/>
    </dbReference>
    <dbReference type="GeneID" id="1452515"/>
    <dbReference type="KEGG" id="mja:MJ_1606"/>
    <dbReference type="eggNOG" id="arCOG01420">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_545885_0_0_2"/>
    <dbReference type="InParanoid" id="Q59001"/>
    <dbReference type="OMA" id="HNEAFKG"/>
    <dbReference type="OrthoDB" id="83551at2157"/>
    <dbReference type="PhylomeDB" id="Q59001"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033201">
        <property type="term" value="F:alpha-1,4-glucan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:glycogen (starch) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009011">
        <property type="term" value="F:starch synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00484">
        <property type="entry name" value="Glycogen_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011835">
        <property type="entry name" value="GS/SS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword id="KW-0328">Glycosyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000188668" description="Probable glycogen synthase">
        <location>
            <begin position="1"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="521" mass="59736" checksum="68B1E0DE40715CC2" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-10-31" modified="2022-08-03" version="100" xmlns="http://uniprot.org/uniprot">
    <accession>Q59015</accession>
    <name>Y1620_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1620</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1620</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64502">
        <property type="entry name" value="C64502"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871145.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59015"/>
    <dbReference type="SMR" id="Q59015"/>
    <dbReference type="STRING" id="243232.MJ_1620"/>
    <dbReference type="EnsemblBacteria" id="AAB99641">
        <property type="protein sequence ID" value="AAB99641"/>
        <property type="gene ID" value="MJ_1620"/>
    </dbReference>
    <dbReference type="GeneID" id="1452529"/>
    <dbReference type="KEGG" id="mja:MJ_1620"/>
    <dbReference type="eggNOG" id="arCOG01356">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_508670_0_0_2"/>
    <dbReference type="InParanoid" id="Q59015"/>
    <dbReference type="OMA" id="GLRPYVY"/>
    <dbReference type="OrthoDB" id="9274at2157"/>
    <dbReference type="PhylomeDB" id="Q59015"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaB/NifB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0004">4Fe-4S</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0411">Iron-sulfur</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="chain" id="PRO_0000107441" description="Uncharacterized protein MJ1620">
        <location>
            <begin position="1"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="2">
        <location>
            <begin position="193"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="435"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01266"/>
        </source>
    </evidence>
    <sequence length="505" mass="57466" checksum="949F2318DF8D7404" modified="1996-11-01" version="1">MKALIIDCLASGDGKRILARDVIGAGPRTVKGILQSEGIEAKITPVEDLDIKDIKKYNLIFISAMTSDFKCVKKLVERIRLKTDSKIIVGGPIANDINILEKIEADISIVGEGEITIRELIKKDFDAEDVKGTTYWDYNEDELKINPLREILTDLKLITPSTEIKDYKNYFSARVYVEVVRGCSNFKRPLLLCTNKKCNLCENGTLKCPLNINPGCGFCSVPSVFGYARSRDEEDILKEVEALLKEGVNRIVLSAPDFLDYKRGEKLINPYFPEPNYEAIESLLSKCKDLADKYNANVLIENIKANLFNEKVAEIFSKYLKTPIYIGCESGDKNHCKLLGRPTTPDDVLKAVKIAKKYNLKAQVYFIYGLPGENEETAKNTVNFMHKIKNYIDKITVYKFRPLPMSAFQNFKPNITKYSLLIRETANKINLEIKKKYIGRVLEVIISERHFRNKRDAIGYLPDSGLMVVVKDGAKFIGKTKKVKIIKAYEKYLEGRILKTWLCKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2002-10-25" modified="2022-08-03" version="79" xmlns="http://uniprot.org/uniprot">
    <accession>Q59019</accession>
    <name>Y1626_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ1626</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1626</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64502">
        <property type="entry name" value="G64502"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59019"/>
    <dbReference type="SMR" id="Q59019"/>
    <dbReference type="STRING" id="243232.MJ_1626"/>
    <dbReference type="PRIDE" id="Q59019"/>
    <dbReference type="EnsemblBacteria" id="AAB99649">
        <property type="protein sequence ID" value="AAB99649"/>
        <property type="gene ID" value="MJ_1626"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_1626"/>
    <dbReference type="eggNOG" id="arCOG08290">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_400448_0_0_2"/>
    <dbReference type="OMA" id="YYSKLAF"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="SSF48371"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107446" description="Uncharacterized protein MJ1626">
        <location>
            <begin position="1"/>
            <end position="765"/>
        </location>
    </feature>
    <sequence length="765" mass="89607" checksum="056EEAA4FEF9B098" modified="1996-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="2003-09-26" modified="2022-05-25" version="98" xmlns="http://uniprot.org/uniprot">
    <accession>Q59023</accession>
    <name>Y1629_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0282 protein MJ1629</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1629</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0282 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64503">
        <property type="entry name" value="C64503"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871153.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59023"/>
    <dbReference type="STRING" id="243232.MJ_1629"/>
    <dbReference type="EnsemblBacteria" id="AAB99650">
        <property type="protein sequence ID" value="AAB99650"/>
        <property type="gene ID" value="MJ_1629"/>
    </dbReference>
    <dbReference type="GeneID" id="1452538"/>
    <dbReference type="KEGG" id="mja:MJ_1629"/>
    <dbReference type="eggNOG" id="arCOG00969">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079268_0_0_2"/>
    <dbReference type="InParanoid" id="Q59023"/>
    <dbReference type="OMA" id="YHYDHHT"/>
    <dbReference type="OrthoDB" id="36408at2157"/>
    <dbReference type="PhylomeDB" id="Q59023"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01406">
        <property type="entry name" value="UPF0282"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014426">
        <property type="entry name" value="UPF0282_hydrls"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF4">
        <property type="entry name" value="PTHR43546:SF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004944">
        <property type="entry name" value="UCP004944_hydrls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="SSF56281"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000057631" description="UPF0282 protein MJ1629">
        <location>
            <begin position="1"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01406"/>
        </source>
    </evidence>
    <sequence length="292" mass="34049" checksum="6F49BB5A3225E13F" modified="1996-11-01" version="1">MKVIPLASESLGVRSLATYVKTKDVGILIDPGVALAPDRYGLKPNDIEFEKLREMRNKINDYAKKSNVITISHYHYDHYTPFFDDIYLESKDYAKELYKDKILLIKHPTEFINKSQMNRAKKFLESVKDIAKKIEFADNKTFKFGKTEIKFSPPFPHGRDDKLGYVLITTVKEGKFKFMHTSDTQGIIFDDIRDYIIKEKPNLILMGGPPTYLMHRYGKKNLEKTNENLKYIVENTGAELIIDHHLLRDKKFREKINVDFKTVAEFLGEKNLLLEAYRKEIKQGKDINELFG</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2004-02-02" modified="2022-08-03" version="87" xmlns="http://uniprot.org/uniprot">
    <accession>Q59051</accession>
    <name>Y1657_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protease MJ1657</fullName>
            <ecNumber>3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ1657</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U32 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB99684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64506">
        <property type="entry name" value="G64506"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010871181.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q59051"/>
    <dbReference type="SMR" id="Q59051"/>
    <dbReference type="STRING" id="243232.MJ_1657"/>
    <dbReference type="PRIDE" id="Q59051"/>
    <dbReference type="DNASU" id="1452566"/>
    <dbReference type="EnsemblBacteria" id="AAB99684">
        <property type="protein sequence ID" value="AAB99684"/>
        <property type="gene ID" value="MJ_1657"/>
    </dbReference>
    <dbReference type="GeneID" id="1452566"/>
    <dbReference type="KEGG" id="mja:MJ_1657"/>
    <dbReference type="eggNOG" id="arCOG03202">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053295_0_0_2"/>
    <dbReference type="InParanoid" id="Q59051"/>
    <dbReference type="OMA" id="HFNFFSH"/>
    <dbReference type="OrthoDB" id="33085at2157"/>
    <dbReference type="PhylomeDB" id="Q59051"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000079187" description="Uncharacterized protease MJ1657">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000305" key="1"/>
    <sequence length="332" mass="38535" checksum="F1DC6C3FC0B62B5A" modified="1996-11-01" version="1">MFSISHPGDFESLKIIVNEINKLRKINKKLKKESFEVYVGFPEFVGTGRATLYKPNFEDLAKQVNYAKKHNVRFEVVINASCIGGMHLTPKGISYINWIFSQLKNIGVDSVALSDPYLVDLAKNNGLEVNVSCIALVDSLDKALFWDEKEVYAITLDSSINRHFDIIQEIRENVSCKLKILVNEACLYKCPMRIQHFNFFSHANAQNIPALDDYYYNKCINLRIKKKELIIKSPFIRPEDLKYYKGLVDIFKISGRSHPIGWIKRVINAYLNERWDGNLMELLDCPRELEHFYYLDNRALDGAIEYWKSCNKLCSKCNFCKELAEKALKVKY</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="59" xmlns="http://uniprot.org/uniprot">
    <accession>Q60271</accession>
    <name>Y3509_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL09</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL09</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="A64511">
        <property type="entry name" value="A64511"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890056.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60271"/>
    <dbReference type="STRING" id="243232.MJ_ECL09"/>
    <dbReference type="EnsemblBacteria" id="AAC37082">
        <property type="protein sequence ID" value="AAC37082"/>
        <property type="gene ID" value="MJ_ECL09"/>
    </dbReference>
    <dbReference type="GeneID" id="1450795"/>
    <dbReference type="KEGG" id="mja:MJ_ECL09"/>
    <dbReference type="HOGENOM" id="CLU_2191038_0_0_2"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107501" description="Uncharacterized protein MJECL09">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <sequence length="108" mass="13308" checksum="482EE7DFA65A277D" modified="1997-11-01" version="1">MNYMKFMWNSEDGFIVEEQHKEEIPFEDWIVNTVEQLKNLQYTRYTFEELTPKTKNLEAYNKLLEILKKHTNMDIENNVKRIYHCIAENNNYKYEIAFYIVISKNWNE</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="101" xmlns="http://uniprot.org/uniprot">
    <accession>Q60291</accession>
    <name>Y3535_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJECL35</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL35</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="B64514">
        <property type="entry name" value="B64514"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890082.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60291"/>
    <dbReference type="STRING" id="243232.MJ_ECL35"/>
    <dbReference type="EnsemblBacteria" id="AAC37104">
        <property type="protein sequence ID" value="AAC37104"/>
        <property type="gene ID" value="MJ_ECL35"/>
    </dbReference>
    <dbReference type="GeneID" id="1450817"/>
    <dbReference type="KEGG" id="mja:MJ_ECL35"/>
    <dbReference type="eggNOG" id="arCOG03235">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025425_0_0_2"/>
    <dbReference type="InParanoid" id="Q60291"/>
    <dbReference type="OMA" id="IRIIFEN"/>
    <dbReference type="OrthoDB" id="38516at2157"/>
    <dbReference type="PhylomeDB" id="Q60291"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="CDD" id="cd01026">
        <property type="entry name" value="TOPRIM_OLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041685">
        <property type="entry name" value="AAA_15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034139">
        <property type="entry name" value="TOPRIM_OLD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13175">
        <property type="entry name" value="AAA_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="SSF52540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000107519" description="Uncharacterized protein MJECL35">
        <location>
            <begin position="1"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="1">
        <location>
            <begin position="412"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00995"/>
        </source>
    </evidence>
    <sequence length="630" mass="72441" checksum="FD8EAE30D2F5B58E" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="86" xmlns="http://uniprot.org/uniprot">
    <accession>Q60294</accession>
    <name>Y3539_METJA</name>
    <protein>
        <recommendedName>
            <fullName>UPF0252 protein MJECL39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJECL39</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <geneLocation type="plasmid">
        <name>large ECE</name>
    </geneLocation>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0252 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77118">
        <property type="protein sequence ID" value="AAC37108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="F64514">
        <property type="entry name" value="F64514"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010890086.1">
        <property type="nucleotide sequence ID" value="NC_001732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60294"/>
    <dbReference type="STRING" id="243232.MJ_ECL39"/>
    <dbReference type="EnsemblBacteria" id="AAC37108">
        <property type="protein sequence ID" value="AAC37108"/>
        <property type="gene ID" value="MJ_ECL39"/>
    </dbReference>
    <dbReference type="GeneID" id="1450821"/>
    <dbReference type="KEGG" id="mja:MJ_ECL39"/>
    <dbReference type="eggNOG" id="arCOG02164">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_722835_0_0_2"/>
    <dbReference type="OMA" id="TNILEWQ"/>
    <dbReference type="OrthoDB" id="120263at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Plasmid large ECE"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007562">
        <property type="entry name" value="Transglutaminase-like_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04473">
        <property type="entry name" value="DUF553"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-0614">Plasmid</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000159556" description="UPF0252 protein MJECL39">
        <location>
            <begin position="1"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="351" mass="41438" checksum="AACBF7C32BC213B0" modified="1997-11-01" version="1">MPILLEHIQLNDEDLKDTKHLAEILKYKNNSIKTIINVLEWEDSNIRYCYEKSNVYYFITFFIIVGLVWAIFPEVWLWCEQVFCISPTIHIIICCLYFIITIILFLFLCGVVGTFLHLWATFFTLSKCDSKILKLKEGLFTTLSLIWISTSLKTILKTKYAICRDYAKLTSAILHNLNIKHYFLVYPTHVAVAVKIDDYYYVIDQKLPIYKIDVWLKKLGKEKVKIYTPVDIYNSKLKFVEKYYKNENNLKSEISDDILRKIEEDVKKELQIKNAEQYNKKVEPIPVKLSIPIENYDEITHYSIVRVISKEIYNKFLTNIKNVSNIEIKKDEGKFAVNVYYEIPNSIPNSK</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="120" xmlns="http://uniprot.org/uniprot">
    <accession>Q60325</accession>
    <name>GATE_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="1">Glu-ADT subunit E</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatE</name>
        <name type="ordered locus">MJ0019</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H(+) + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB97995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64302">
        <property type="entry name" value="C64302"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869511.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60325"/>
    <dbReference type="SMR" id="Q60325"/>
    <dbReference type="STRING" id="243232.MJ_0019"/>
    <dbReference type="PRIDE" id="Q60325"/>
    <dbReference type="EnsemblBacteria" id="AAB97995">
        <property type="protein sequence ID" value="AAB97995"/>
        <property type="gene ID" value="MJ_0019"/>
    </dbReference>
    <dbReference type="GeneID" id="1450857"/>
    <dbReference type="KEGG" id="mja:MJ_0019"/>
    <dbReference type="eggNOG" id="arCOG01719">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030702_0_0_2"/>
    <dbReference type="InParanoid" id="Q60325"/>
    <dbReference type="OMA" id="SGFQRTM"/>
    <dbReference type="OrthoDB" id="35861at2157"/>
    <dbReference type="PhylomeDB" id="Q60325"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="PTHR11659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="PTHR11659:SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="SSF55261"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="SSF55931"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="SSF89095"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0436">Ligase</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0648">Protein biosynthesis</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000140072" description="Glutamyl-tRNA(Gln) amidotransferase subunit E">
        <location>
            <begin position="1"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <sequence length="630" mass="71225" checksum="43D401B61B88F7AF" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="118" xmlns="http://uniprot.org/uniprot">
    <accession>Q60348</accession>
    <name>Y043_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0043</fullName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName>Mja hyp1 intein</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name type="ordered locus">MJ0043</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="PTM">
        <text evidence="3">This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64305">
        <property type="entry name" value="C64305"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869534.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60348"/>
    <dbReference type="SMR" id="Q60348"/>
    <dbReference type="STRING" id="243232.MJ_0043"/>
    <dbReference type="EnsemblBacteria" id="AAB98023">
        <property type="protein sequence ID" value="AAB98023"/>
        <property type="gene ID" value="MJ_0043"/>
    </dbReference>
    <dbReference type="GeneID" id="1450881"/>
    <dbReference type="KEGG" id="mja:MJ_0043"/>
    <dbReference type="eggNOG" id="arCOG04881">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_357040_0_0_2"/>
    <dbReference type="InParanoid" id="Q60348"/>
    <dbReference type="OMA" id="LIGPAHC"/>
    <dbReference type="OrthoDB" id="29545at2157"/>
    <dbReference type="PhylomeDB" id="Q60348"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005287">
        <property type="entry name" value="CHP00375"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="SSF51294"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="SSF89550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="TIGRFAMs" id="TIGR00375">
        <property type="entry name" value="TIGR00375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword id="KW-0651">Protein splicing</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000013988" description="Uncharacterized protein MJ0043, 1st part" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013989" description="Mja hyp1 intein" evidence="1">
        <location>
            <begin position="129"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000013990" description="Uncharacterized protein MJ0043, 2nd part" evidence="1">
        <location>
            <begin position="521"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="2">
        <location>
            <begin position="293"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000255" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="3"/>
    <sequence length="785" mass="91459" checksum="4167A59032511F17" modified="1997-11-01" version="1"> </sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-08-03" version="115" xmlns="http://uniprot.org/uniprot">
    <accession>Q60351</accession>
    <name>RPO4_METJA</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo4</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1 3">DNA-directed RNA polymerase subunit F</fullName>
            <shortName evidence="3">mjF</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo4</name>
        <name evidence="1" type="synonym">rpoF</name>
        <name type="ordered locus">MJ0039</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2000" name="Nucleic Acids Res." volume="28" first="4299" last="4305">
            <title>Archaeal RNA polymerase subunits F and P are bona fide homologs of eukaryotic RPB4 and RPB12.</title>
            <authorList>
                <person name="Werner F."/>
                <person name="Eloranta J.J."/>
                <person name="Weinzierl R.O."/>
            </authorList>
            <dbReference type="PubMed" id="11058130"/>
            <dbReference type="DOI" id="10.1093/nar/28.21.4299"/>
        </citation>
        <scope>INTERACTION WITH RPO7</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="journal article" date="2001" name="Mol. Cell" volume="8" first="1137" last="1143">
            <title>Structure of an archaeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex.</title>
            <authorList>
                <person name="Todone F."/>
                <person name="Brick P."/>
                <person name="Werner F."/>
                <person name="Weinzierl R.O."/>
                <person name="Onesti S."/>
            </authorList>
            <dbReference type="PubMed" id="11741548"/>
            <dbReference type="DOI" id="10.1016/s1097-2765(01)00379-3"/>
        </citation>
        <scope>X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 9-115</scope>
        <scope>SUBUNIT</scope>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit is less well bound than the others.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 2 4">Part of the RNA polymerase complex. Forms a stalk with Rpo7 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">It is uncertain whether Met-1 or Met-9 is the initiator.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="G64304">
        <property type="entry name" value="G64304"/>
    </dbReference>
    <dbReference type="PDB" id="1GO3">
        <property type="method" value="X-ray"/>
        <property type="resolution" value="1.75 A"/>
        <property type="chains" value="F/N=9-115"/>
    </dbReference>
    <dbReference type="PDBsum" id="1GO3"/>
    <dbReference type="AlphaFoldDB" id="Q60351"/>
    <dbReference type="SMR" id="Q60351"/>
    <dbReference type="IntAct" id="Q60351">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="243232.MJ_0039"/>
    <dbReference type="EnsemblBacteria" id="AAB98020">
        <property type="protein sequence ID" value="AAB98020"/>
        <property type="gene ID" value="MJ_0039"/>
    </dbReference>
    <dbReference type="KEGG" id="mja:MJ_0039"/>
    <dbReference type="eggNOG" id="arCOG01016">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_165892_1_0_2"/>
    <dbReference type="InParanoid" id="Q60351"/>
    <dbReference type="OMA" id="VDIMPED"/>
    <dbReference type="PhylomeDB" id="Q60351"/>
    <dbReference type="EvolutionaryTrace" id="Q60351"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription, initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00864">
        <property type="entry name" value="RNApol_arch_Rpo4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010924">
        <property type="entry name" value="Rpo4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646">
        <property type="entry name" value="PTHR39646"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005053">
        <property type="entry name" value="RNA_pol_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="SSF47819"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="evidence at protein level"/>
    <keyword id="KW-0002">3D-structure</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0804">Transcription</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000106663" description="DNA-directed RNA polymerase subunit Rpo4">
        <location>
            <begin position="1"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="strand" evidence="7">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="22"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="57"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="88"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="helix" evidence="7">
        <location>
            <begin position="104"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000269" key="2">
        <source>
            <dbReference type="PubMed" id="11741548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000303" key="3">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4">
        <source>
            <dbReference type="PubMed" id="11058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="5">
        <source>
            <dbReference type="PubMed" id="11741548"/>
        </source>
    </evidence>
    <evidence type="ECO:0007744" key="6">
        <source>
            <dbReference type="PDB" id="1GO3"/>
        </source>
    </evidence>
    <evidence type="ECO:0007829" key="7">
        <source>
            <dbReference type="PDB" id="1GO3"/>
        </source>
    </evidence>
    <sequence length="115" mass="13389" checksum="898CE40770B73730" modified="1997-11-01" version="1">MYQAKRERMIGKKILGERYVTVSEAAEIMYNRAQIGELSYEQGCALDYLQKFAKLDKEEAKKLVEELISLGIDEKTAVKIADILPEDLDDLRAIYYKRELPENAEEILEIVRKYI</sequence>
</entry>
<entry dataset="Swiss-Prot" created="1997-11-01" modified="2022-05-25" version="90" xmlns="http://uniprot.org/uniprot">
    <accession>Q60371</accession>
    <name>Y067_METJA</name>
    <protein>
        <recommendedName>
            <fullName>Uncharacterized protein MJ0067</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="ordered locus">MJ0067</name>
    </gene>
    <organism>
        <name type="scientific">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</name>
        <name type="synonym">Methanococcus jannaschii</name>
        <dbReference type="NCBI Taxonomy" id="243232"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Euryarchaeota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="1996" name="Science" volume="273" first="1058" last="1073">
            <title>Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii.</title>
            <authorList>
                <person name="Bult C.J."/>
                <person name="White O."/>
                <person name="Olsen G.J."/>
                <person name="Zhou L."/>
                <person name="Fleischmann R.D."/>
                <person name="Sutton G.G."/>
                <person name="Blake J.A."/>
                <person name="FitzGerald L.M."/>
                <person name="Clayton R.A."/>
                <person name="Gocayne J.D."/>
                <person name="Kerlavage A.R."/>
                <person name="Dougherty B.A."/>
                <person name="Tomb J.-F."/>
                <person name="Adams M.D."/>
                <person name="Reich C.I."/>
                <person name="Overbeek R."/>
                <person name="Kirkness E.F."/>
                <person name="Weinstock K.G."/>
                <person name="Merrick J.M."/>
                <person name="Glodek A."/>
                <person name="Scott J.L."/>
                <person name="Geoghagen N.S.M."/>
                <person name="Weidman J.F."/>
                <person name="Fuhrmann J.L."/>
                <person name="Presley E.A."/>
                <person name="Nguyen D."/>
                <person name="Utterback T.R."/>
                <person name="Kelley J.M."/>
                <person name="Peterson J.D."/>
                <person name="Sadow P.W."/>
                <person name="Hanna M.C."/>
                <person name="Cotton M.D."/>
                <person name="Hurst M.A."/>
                <person name="Roberts K.M."/>
                <person name="Kaine B.P."/>
                <person name="Borodovsky M."/>
                <person name="Klenk H.-P."/>
                <person name="Fraser C.M."/>
                <person name="Smith H.O."/>
                <person name="Woese C.R."/>
                <person name="Venter J.C."/>
            </authorList>
            <dbReference type="PubMed" id="8688087"/>
            <dbReference type="DOI" id="10.1126/science.273.5278.1058"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <dbReference type="EMBL" id="L77117">
        <property type="protein sequence ID" value="AAB98052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="PIR" id="C64308">
        <property type="entry name" value="C64308"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_010869559.1">
        <property type="nucleotide sequence ID" value="NC_000909.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="Q60371"/>
    <dbReference type="STRING" id="243232.MJ_0067"/>
    <dbReference type="DNASU" id="1450906"/>
    <dbReference type="EnsemblBacteria" id="AAB98052">
        <property type="protein sequence ID" value="AAB98052"/>
        <property type="gene ID" value="MJ_0067"/>
    </dbReference>
    <dbReference type="GeneID" id="1450906"/>
    <dbReference type="KEGG" id="mja:MJ_0067"/>
    <dbReference type="eggNOG" id="arCOG04811">
        <property type="taxonomic scope" value="Archaea"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095425_1_2_2"/>
    <dbReference type="InParanoid" id="Q60371"/>
    <dbReference type="OMA" id="FWLQQKG"/>
    <dbReference type="OrthoDB" id="124471at2157"/>
    <dbReference type="Proteomes" id="UP000000805">
        <property type="component" value="Chromosome"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016021">
        <property type="term" value="C:integral component of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-1003">Cell membrane</keyword>
    <keyword id="KW-0472">Membrane</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0812">Transmembrane</keyword>
    <keyword id="KW-1133">Transmembrane helix</keyword>
    <feature type="chain" id="PRO_0000106677" description="Uncharacterized protein MJ0067">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1"/>
    <evidence type="ECO:0000305" key="2"/>
    <sequence length="103" mass="11059" checksum="94DCD5C2F9394D53" modified="1997-11-01" version="1">MIILDFSQFLPDIGSGFIIGFVIGWAAKKAIKVVAFLIGIYILSLLYLAKIGVISINKEAFSALLGNLENSLLVFGDKIIGLIHSFSFGTSFLIGFGLGFKKG</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>